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Sulfonamide derivatives with benzothiazole scaffold: Synthesis and carbonic anhydrase I –II inhibition properties
AbstractThe secondary sulfonamide derivatives containing benzothiazole scaffold (1 –10) were synthesized to determine their inhibition properties on two physiologically essential human carbonic anhydrases isoforms (hCAs, EC, 4.2.1.1), hCA I, and hCA II. The inhibitory effects of the compounds on hCA I and hCA II isoenzymes were investigated by comparing their IC50 andKi values. TheKi values of compounds (1 –10) against hCA I and hCA II are in the range of 0.052  ± 0.022–0.971 ± 0.280 and 0.025 ± 0.010–0.682 ± 0.335, respectively. Some of these inhibited the enzyme more effectively than the standard drug...
Source: Biotechnology and Applied Biochemistry - November 15, 2023 Category: Biochemistry Authors: Cansu Öztürk, Erbay Kalay, Serpil Gerni, Neslihan Balci, Feyzi Sinan Tokali, Osman Nuri Aslan, Emrah Polat Tags: ORIGINAL ARTICLE Source Type: research

Inhibition of USP21 leads to ovarian carcinoma cell death by suppressing MAPK signaling
AbstractOvarian cancer is the most aggressive and lethal of all gynecologic malignancies. Although the overexpression (OE) of ubiquitin-specific peptidase 21 (USP21) has been observed in multiple cancers, its expression profile and biological function in ovarian cancer remain unknown. The expression levels of USP21 in ovarian cancer cells and tissues as well as adjacent normal tissues were assessed by qRT-PCR or Western blot assay. The biological function of USP21 in ovarian cancer cells was assessed by cell growth assay in vitro and a tumor growth model in vivo. Our study revealed that USP21 was markedly elevated in ovari...
Source: Biotechnology and Applied Biochemistry - November 15, 2023 Category: Biochemistry Authors: Xin Sun, Jia Yu, Xiaorong Cui, Yujie Tang, Yani Yu Tags: ORIGINAL ARTICLE Source Type: research

Sequence identification and in silico characterization of novel thermophilic lipases from Geobacillus species
In this study, we cloned and sequenced Lip21 and Lip33 genes fromGeobacillus sp. GS21 andGeobacillus sp. GS33, then we in silico and experimentally analyzed the encoded lipases. For this purpose, Lip21 and Lip33 were cloned, sequenced, and their amino acid sequences were investigated for determination of biophysicochemical characteristics, evolutionary relationships, and sequence similarities. 3D models were built and computationally affirmed by various bioinformatics tools, and enzyme-ligand interactions were investigated by docking analysis using six ligands. Biophysicochemical property of Lip21 and Lip33 was also determ...
Source: Biotechnology and Applied Biochemistry - November 13, 2023 Category: Biochemistry Authors: Yusuf S ürmeli, Hasan Cihad Tekedar, Gülşah Şanlı‐Mohamed Tags: ORIGINAL ARTICLE Source Type: research

A two ‐step electrochemical biosensor based on Tetrazyme for the detection of fibrin
In this study, an electrochemical biosensor was constructed for the detection of fibrin, specifically by a simple two-step approach, with a novel artificial enzyme (Tetrazyme) based on the DNA tetrahedral framework as signal probe. The multichannel screen-printed electrode with the activated surface cannot only remove some biological impurities, but also serve as a carrier to immobilize a large number of antigen proteins. The DNA tetrahedral nanostructure was employed to ensure the high sensitivity of the probe for biological analysis. The hemin was chimeric into the G-quadruplex to constitute the complex with peroxidase c...
Source: Biotechnology and Applied Biochemistry - October 31, 2023 Category: Biochemistry Authors: Jiayi Jiang, Bin Wang, Linghuan Luo, Na Ying, Gaofan Shi, Mengmeng Zhang, Haoyuan Su, Dongdong Zeng Tags: ORIGINAL ARTICLE Source Type: research

Development of a new affinity chromatography method for purification of horseradish peroxidase enzyme
In this study, benzohydroxamic acid molecules were synthesized from methyl 4-amino-2-methoxy, methyl 4-amino-3-nitro, methyl 4-amino-3-methyl, and methyl 4-amino-3-chloro benzoate molecules, and the horseradish peroxidase (HRP) enzyme was purified in one step using the affinity chromatography technique for the first time. The IC50 andKi values for the 4-amino 3-methyl benzohydroxamic acid molecule were 0.136 and 0.132 ± 0.054 μM, respectively, while the IC50 andKi values for the 4-amino-3-nitro benzohydroxamic acid molecule were 56.00 and 51.90 ± 9.90 μM, respectively. It was found that the IC50 andKi values for the ...
Source: Biotechnology and Applied Biochemistry - October 31, 2023 Category: Biochemistry Authors: Serpil Gerni, Hasan Özdemir Tags: ORIGINAL ARTICLE Source Type: research

Homology modeling, virtual screening, molecular docking, and ADME approaches to identify a potent agent targeting NK2R protein
AbstractNeurokinin/tachykinin receptors are classified as the G-protein coupled receptor superfamily. The neurokinin 2 receptor (NK2R) is widely expressed in different tissues. NK2R is associated with a range of biological events, such as inflammation, smooth muscle contraction, intestinal motor functions, and asthma. Despite these diverse activities, no approved drugs targeting NK2R have been developed yet. Our study focuses on finding potential inhibitors for NK2R using virtual screening, molecular docking, and ADME (absorption, distribution, metabolism, and excretion) approaches. We used a homology modeling approach and...
Source: Biotechnology and Applied Biochemistry - October 31, 2023 Category: Biochemistry Authors: Arash Soltani, Seyed Isaac Hashemy Tags: ORIGINAL ARTICLE Source Type: research

Protective effect of human epicardial adipose ‐derived stem cells on myocardial injury driven by poly‐lactic acid nanopillar array
AbstractWe investigated if poly-lactic acid (PLA) nanopillar array can trigger the differentiation of human epicardial (ADSCs) (heADSCs) into cardiomyocyte-like cells and explored the effects of these cardiomyocyte-like cells on myocardial infarction (MI) in vivo. PLA nanopillar array (200  nm diameter) and plain PLA film (PLA planar) induced heADSCs were marked with carboxyfluorescein. After 7 days, the expressions of myocardiocyte-specific genes were significantly enhanced in cells seeded on PLA nanopillar array compared with that on PLA planar, especiallyCACNA1C,KCNH2, andMYL2 genes (p <  0.05). However, the expre...
Source: Biotechnology and Applied Biochemistry - October 31, 2023 Category: Biochemistry Authors: Wenju Yan, Yan Li, Jie Yin, Qian Liu, Yugen Shi, Jiayu Tan, Yu Wang, Shan Zhang, Junyi Zhang, Jingxin Li, Suhua Yan Tags: ORIGINAL ARTICLE Source Type: research

Identification of angiogenesis ‐related subtypes, the development of a prognosis model, and features of tumor microenvironment in colon cancer
AbstractAngiogenesis is associated with tumor progression, prognosis, and treatment effect. However, the angiogenesis ’ underlying mechanisms in the tumor microenvironment (TME) still remain unclear. Understanding the dynamic interactions between angiogenesis and TME in colon adenocarcinoma (COAD) is necessary. We downloaded the transcriptome data and corresponding clinical data of colon cancer patients from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases, respectively. We identified two distinct angiogenesis-related molecular subtypes (subtype A and subtype B) and assessed the clinical fea...
Source: Biotechnology and Applied Biochemistry - October 26, 2023 Category: Biochemistry Authors: Feifei Wang, Changjing Wang, Baokun Li, Guanglin Wang, Zesong Meng, Jiachao Han, Ganlin Guo, Bin Yu, Guiying Wang Tags: ORIGINAL ARTICLE Source Type: research