Biotechnology and Applied Biochemistry 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Nanoliposome ‐loaded phenolics from Salvia leriifolia Benth and its anticancer effects against induced colorectal cancer in mice
The objective of this study is to assess the therapeutic potential of phenolic-rich fraction encapsulated nanoliposome (PRF-NLs) ofSalvia leriifolia Benth in the treatment of colon cancer in mice. Initially, the phenolic-rich fraction (PRF) was extracted and then encapsulated into nanoliposomes. The physicochemical properties of the nanoliposomes were evaluated using dynamic light scattering, zeta potential, and field emission scanning electron microscopy. Subsequently, 24 mice with HT-29 colon cancer cells were divided into three groups, and the anticancer effects of PRF-NLs were measured. The results showed that the ethy...
Source: Biotechnology and Applied Biochemistry - February 8, 2024 Category: Biochemistry Authors: Niusha Shojaee Nasrabadi,
Arezoo Vedad,
Kimia Asadi,
Mahsa Rastegar Moghaddam Poorbagher,
Nadia Abdollahian Tabrizi,
Kiyana Dorooki,
Roghayeh Sabouhi Sabouni,
Mahla barati moghadam,
Negin Shafaei,
Ehsan Karimi,
Ehsan Oskoueian Tags: ORIGINAL ARTICLE Source Type: research
HPLC purification of antioxidant and antibacterial peptides from a lichen “Parmotrema perlatum (Huds.) M. Choisy”: Identification by LC‐MS/MS peptide mass fingerprinting
AbstractParmotrema perlatum, a lichen belonging to the family Parmeliaceae, is well known for its culinary benefits and aroma used as a condiment in Indian homes is also known as the “black stone flower” or “kalpasi” in India. This research intends to analyze the antioxidant power of the crude extracts using four pH-based buffers solubilized proteins/peptides and RP-HPLC fractions ofP. perlatum obtained by purification. The proteins that were extracted from the four different buffers were examined using LC-MS/MS-based peptide mass fingerprinting. When compared to the other buffers, the 0.1 M of Tris-HCl buffer pH...
Source: Biotechnology and Applied Biochemistry - February 5, 2024 Category: Biochemistry Authors: Dwarakanath P. Radhakrishnan,
Abinaya Kanakaraja,
Nagasathiya Krishnan,
Meenakumari Sakthivelu,
Subash C. B. Gopinath,
Raman Pachaiappan Tags: ORIGINAL ARTICLE Source Type: research
Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis
In this study, the transcriptome analysis was practiced to identify potential genes of probioticBacillus subtilis BSN313 involved in selenium (Se) enrichment metabolism. The transcriptomic variation of the strain was deliberated in presence of three different sodium selenite concentrations (0, 3, and 20 μg/mL). The samples were taken at 1 and 13 h subsequent to inoculation of selenite and gene expression profiles in Se metabolism were analyzed through RNA sequencing. The gene expression levels of the pre log phase were lower than the stationary phase. It is because, the bacteria has maximum gro wn with high concentrati...
Source: Biotechnology and Applied Biochemistry - February 5, 2024 Category: Biochemistry Authors: Asad Ullah,
Xian Yin,
Muhammad Naveed,
Sadar Aslam,
Malik Wajid Hussain Chan,
Sun Bo,
Fenghuan Wang,
Bo Xu,
Baocai Xu,
Zhou Yu Tags: ORIGINAL ARTICLE Source Type: research
HPLC purification of antioxidant and antibacterial peptides from a lichen “Parmotrema perlatum (Huds.) M. Choisy”: Identification by LC‐MS/MS peptide mass fingerprinting
AbstractParmotrema perlatum, a lichen belonging to the family Parmeliaceae, is well known for its culinary benefits and aroma used as a condiment in Indian homes is also known as the “black stone flower” or “kalpasi” in India. This research intends to analyze the antioxidant power of the crude extracts using four pH-based buffers solubilized proteins/peptides and RP-HPLC fractions ofP. perlatum obtained by purification. The proteins that were extracted from the four different buffers were examined using LC-MS/MS-based peptide mass fingerprinting. When compared to the other buffers, the 0.1 M of Tris-HCl buffer pH...
Source: Biotechnology and Applied Biochemistry - February 5, 2024 Category: Biochemistry Authors: Dwarakanath P. Radhakrishnan,
Abinaya Kanakaraja,
Nagasathiya Krishnan,
Meenakumari Sakthivelu,
Subash C. B. Gopinath,
Raman Pachaiappan Tags: ORIGINAL ARTICLE Source Type: research
Study of selenium enrichment metabolomics in Bacillus subtilis BSN313 via transcriptome analysis
In this study, the transcriptome analysis was practiced to identify potential genes of probioticBacillus subtilis BSN313 involved in selenium (Se) enrichment metabolism. The transcriptomic variation of the strain was deliberated in presence of three different sodium selenite concentrations (0, 3, and 20 μg/mL). The samples were taken at 1 and 13 h subsequent to inoculation of selenite and gene expression profiles in Se metabolism were analyzed through RNA sequencing. The gene expression levels of the pre log phase were lower than the stationary phase. It is because, the bacteria has maximum gro wn with high concentrati...
Source: Biotechnology and Applied Biochemistry - February 5, 2024 Category: Biochemistry Authors: Asad Ullah,
Xian Yin,
Muhammad Naveed,
Sadar Aslam,
Malik Wajid Hussain Chan,
Sun Bo,
Fenghuan Wang,
Bo Xu,
Baocai Xu,
Zhou Yu Tags: ORIGINAL ARTICLE Source Type: research
Optimization and chemical free fabrication of green synthesized iron nanoparticles as potential MRI contrast agent
AbstractThe current research article has investigated the synthesis and characterization of novel iron nanoparticles (INPs) from neem and betel leaves extract combination using response surface methodology –central composite design and coated with chitosan–curcumin (CCINPs) as a biocompatible and contrast agent for magnetic resonance imaging (MRI). The coating of INPs with chitosan and curcumin (CCINPs) was carried out using a simple, easy, chemical-free ultrasonication method and characteristics were confirmed by UV–visible (Vis) spectrophotometer (UV–Vis), Fourier-transform infrared spectroscopy, X-ray diffractio...
Source: Biotechnology and Applied Biochemistry - February 2, 2024 Category: Biochemistry Authors: Yuwvaranni S,
Punitha N,
Chamundeeswari M Tags: ORIGINAL ARTICLE Source Type: research
Improving the substrate binding of acetyl ‐CoA carboxylase (AccB) from Streptomyces antibioticus through computational enzyme engineering
AbstractMalonyl-CoA serves as the main building block for the biosynthesis of many important polyketides, as well as fatty acid –derived compounds, such as biofuel.Escherichia coli,Corynebacterium gultamicum, andSaccharomyces cerevisiae have recently been engineered for the biosynthesis of such compounds. However, the developed processes and strains often have insufficient productivity. In the current study, we used enzyme-engineering approach to improve the binding of acetyl-CoA with ACC. We generated different mutations, and the impact was calculated, which reported that three mutations, that is, S343A, T347W, and S350...
Source: Biotechnology and Applied Biochemistry - January 30, 2024 Category: Biochemistry Authors: Imtiaz Ali,
Dong ‐Qing Wei,
Abbas Khan,
Yuanyuan Feng,
Muhammad Waseem,
Zahid Hussain,
Arshad Iqbal,
Syed Shujait Ali,
Anwar Mohammad,
Jianting Zheng Tags: ORIGINAL ARTICLE Source Type: research
Analysis of genes specific to the early maturation stage of Sesamum indicum seeds by subtraction method*,**
AbstractThe mechanisms regulating the content ratio of unsaturated fatty acid in sesame oil need to be clarified in order to breed novel varieties with high contents of unsaturated fatty acids. Full-length cDNA libraries prepared from sesame seeds 1 to 3 weeks after flowering were subtracted with cDNAs from plantlets of 4 weeks after germination. A total of 1545 cDNA clones was sequenced. The functions of novel genes expressed specifically during the early maturation of sesame seeds were investigated by the transformation ofArabidopsis thaliana. Thirteen genes for a transcription factor were identified, four of which were ...
Source: Biotechnology and Applied Biochemistry - January 29, 2024 Category: Biochemistry Authors: Yu Okuno,
Akihiro Kishikawa,
Hisashi Imakouji,
Motonobu Yoshida Tags: ORIGINAL ARTICLE Source Type: research
Spontaneous nanoemulsification of cinnamon essential oil: Formulation, characterization, and antibacterial and antibiofilm activity against fish spoilage caused by Serratia rubidaea BFMO8
This study describes the formulation of a CEO nanoemulsion (CEONE) by spontaneous emulsification and evaluates its antibacterial and antibiofilm properties against biofilm-formingSerratia rubidaea BFMO8 isolated from spoiled emperor fish (Lethrinus miniatus). Bacteria causing spoilage in emperor fish were isolated and identified asS. rubidaea using common morphological, cultural, and 16S RNA sequencing methods, and their ability to form biofilms and their susceptibility to CEONE were assessed using biofilm-specific methods. The spontaneous emulsification formulation of CEONE was accomplished using water and Tween 20 surfac...
Source: Biotechnology and Applied Biochemistry - January 23, 2024 Category: Biochemistry Authors: Jasmin Suriya Arul Raj,
Sheena Aliyas,
Yolin Angel Poomany Arul Soundara Rajan,
Kasi Murugan,
Ponmurugan Karuppiah,
Natarajan Arumugam,
Abdulrahman I. Almansour,
Perumal Karthikeyan Tags: ORIGINAL ARTICLE Source Type: research
Intramyocardial delivery of injectable hydrogel with arctigenin alleviated myocardial ischemia –reperfusion injury in rats
AbstractArctigenin belongs to a major bioactive component ofFructus arctii and has been found with cardioprotective effects on rats with ischemia ‒reperfusion (I/R) injury. The application of arctigenin is limited due to poor water solubility and low bioavailability. Hydrogel drug delivery systems can improve the efficacy and safety of drugs, increase drug utilization, and reduce side effects. We hypothesized that hydrogels containing arcti genin would facilitate the effect of arctigenin and alleviate I/R injury in the rat heart. Presently, adult Sprague–Dawley (SD) rats were subjected to 1 h of I/R injury, then hydro...
Source: Biotechnology and Applied Biochemistry - January 22, 2024 Category: Biochemistry Authors: Chengyin Liu,
Xuejun Jiang,
Lanyu Liang,
Han Liu,
Li Li,
Qing Shan Tags: ORIGINAL ARTICLE Source Type: research
