Innovations in dengue virus detection: An overview of conventional and electrochemical biosensor approaches
AbstractGlobally, people are in great threat due to the highly spreading of viral infectious diseases. Every year like 100 –300 million cases of infections are found, and among them, above 80% are not recognized and irrelevant. Dengue virus (DENV) is an arbovirus infection that currently infects people most frequently. DENV encompasses four viral serotypes, and they each express comparable sign. From a mild febrile si ckness to a potentially fatal dengue hemorrhagic fever, dengue can induce a variety of symptoms. Presently, the globe is being challenged by the untimely identification of dengue infection. Therefore, this ...
Source: Biotechnology and Applied Biochemistry - January 16, 2024 Category: Biochemistry Authors: Shadan Raza, Renu Poria, Deepak Kala, Nishant Sharma, Anil K. Sharma, Nkurunziza Florien, Hardeep S. Tuli, Ankur Kaushal, Shagun Gupta Tags: REVIEW ARTICLE Source Type: research

Enzymatic properties of ornithine decarboxylase from Clostridium aceticum DSM1496
In this study, we expressed ODC derived fromC. aceticum DSM1496 inEscherichia coli BL21 (DE3) and thoroughly examined its enzymatic properties. The enzyme has a molecular weight of 55.27  kDa and uses pyridoxal-5′-phosphate (PLP) as a coenzyme with aKm = 0.31 mM. ODC exhibits optimal activity at pH 7.5, and it maintains high stability even at pH 4.5. The peak reaction temperature for ODC is 30°C. Besides, it can be influenced by certain metal ions such as Mn2+. Althoughl-ornithine serves as the preferred substrate for ODC, the enzyme also decarboxylatesl-arginine andl-lysine simultaneously. The results indicate that...
Source: Biotechnology and Applied Biochemistry - January 16, 2024 Category: Biochemistry Authors: Qian Tan, Linbo Gou, Tai ‐Ping Fan, Yujie Cai Tags: ORIGINAL ARTICLE Source Type: research

Production of the prolyl endoprotease (PEP) from Aspergillus sp. FSDE 16 by solid ‐state fermentation (SSF) and use for producing a gluten‐free beer
AbstractBeer is a beverage that contains gluten and cannot be consumed by people with celiac disease. In this context, the enzyme prolyl endoprotease (PEP) can be used to reduce the gluten content in beer. The present study aimed to produce the PEP fromAspergillus sp. FSDE 16 using solid-state fermentation with 5 conditions and comparing with a similar commercial enzyme produced fromAspergillus niger in the production of a gluten-free beer. The results of the performed cultures showed that during the culture, the most increased protease activity (54.46  U/mL) occurred on the 4th day. In contrast, for PEP, the highest acti...
Source: Biotechnology and Applied Biochemistry - January 12, 2024 Category: Biochemistry Authors: Tha ís Cartaxo de Almeida, Sharline Florentino de Melo Santos, Everaldo Silvino dos Santos Tags: ORIGINAL ARTICLE Source Type: research

In vitro and in vivo assessment of antimicrobial, enzymatic, and antifouling properties of self ‐potent lichen symbiotic bacteria
In conclusion, the lichen bacterial symbiotic isolateB. proteolyticus exhibited significant in vitro and in vivo inhibition of foulants. This study highlights the potential of lichens as a valuable source of yet unexplored bacteria. The bacterial consortium associated withParmotrema sp. holds promise in combatting biofouling, which poses a substantial threat to the maritime industries and their economic stability. (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - January 8, 2024 Category: Biochemistry Authors: Rubavathi Subbaiyan, Ayyappadasan Ganesan Tags: ORIGINAL ARTICLE Source Type: research

Extraction process, physicochemical properties, and digestive performance of red yeast rice starch
AbstractIn the present study, taking red yeast rice (RYR) as the raw material, the optimum extraction process of RYR starch was investigated through a single-factor experiment and the Box –Behnken design: The liquid–to-solid ratio was 5 mL/g, the concentration of sodium hydroxide solution was 0.075 mol/L, and the extraction time was 3.1 h. Under these extraction conditions, the extraction rate of starch reached 90.077%. To explore the influence of solid-state fermentation on R YR starch, three different fermentation stages of RYR starch, raw rice starch, semi-gelatinized rice starch, and RYR starch were used as test...
Source: Biotechnology and Applied Biochemistry - December 22, 2023 Category: Biochemistry Authors: Mengting Bai, Kai Liu, Yulin Wang, Shuguang Hou, Xiaofang Li, Jia Luo Tags: ORIGINAL ARTICLE Source Type: research

Mucilage ‐assisted fabrication of molybdenum trioxide nanostructures for photothermal ablation of breast cancer cells
AbstractNanostructures have been used for various biomedical applications due to their optical, antibacterial, magnetic, antioxidant, and biocompatible properties. Cancer is a prevalent disease that severely threatens human life and health. Thus, innovative and effective therapeutic approaches are urgently required for cancer. Photothermal therapy (PTT) is a promising approach to killing cancer cells. In this investigation, we developed a low-cost, simple, green technique to fabricate molybdenum trioxide nanostructures (MNs) usingOpuntia ficus-indica mucilage as a template. Moreover, the MNs were functionalized with folic ...
Source: Biotechnology and Applied Biochemistry - December 20, 2023 Category: Biochemistry Authors: Reem A. Alomari, Jegan Athinarayanan, Vaiyapuri Subbarayan Periasamy, Ali A. Alshatwi Tags: ORIGINAL ARTICLE Source Type: research

Improving the capturing ability of swirl ‐based microfluidic chip by introducing baffle wall
AbstractMicrofluidics technology is promising in developing microparticle manipulation technology due to its nondestructive control and notable adaptability. The manipulation of microparticle based on swirling stagnation point is one of the feasible microfluidics biotechnologies. Aiming to improve the regulation and control of microparticle, baffle wall is introduced into the 2-microchannel flow field. The theory of wall attachment jet is employed to elucidate the effect of baffle wall. Subsequently, finite volume method simulation is conducted by modeling the swirling flow region (SFR), and the swirling strength is calcul...
Source: Biotechnology and Applied Biochemistry - December 13, 2023 Category: Biochemistry Authors: Yanping Dang, Qin Zhang, Zhiming Ou, Shuai Hu Tags: ORIGINAL ARTICLE Source Type: research

Resveratrol ‐loaded decellularized ovine pericardium: ECM introduced for tissue engineering
AbstractAn ideal scaffold for skin tissue engineering should have a suitable potential for antibacterial activity, no hemolysis, sufficient porosity for air exchange, water retention capacity, and a suitable swelling rate to maintain tissue moisture. Considering this issue, our study used decellularized ovine pericardial tissue's extracellular matrix (ECM). These scaffolds were decellularized with sodium dodecyl sulfate (SDS) and sodium deoxycholate (SD) detergents along with vacuum methods. Following imaging with scanning electron microscopy (SEM), analysis of the mechanical properties, and the measurement of the amount o...
Source: Biotechnology and Applied Biochemistry - December 12, 2023 Category: Biochemistry Authors: Mozafar Khazaei, Morteza Alizadeh, Leila Rezakhani Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
(Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - December 11, 2023 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research

Inhibition of biofilm, quorum ‐sensing, and swarming motility in pathogenic bacteria by magnetite, manganese ferrite, and nickel ferrite nanoparticles
AbstractResistance to antibiotics by pathogenic bacteria constitutes a health burden and nanoparticles (NPs) are being developed as alternative and multipurpose antimicrobial substances. Magnetite (Fe3O4 np), manganese ferrite (MnFe2O4 np) and nickel ferrite (NiFe3O4 np) NPs were synthesized and characterized using thermogravimetric analysis, transmission electron microscopy, Fourier transformed infra-red, and X-ray diffraction. The minimal inhibitory concentrations (MIC) ranged from 0.625 to 10  mg/mL against gram-positive (Staphylococcus aureus ATCC 25923 andEnterococcus faecalis ATCC 29212), gram-negative (Escherichia ...
Source: Biotechnology and Applied Biochemistry - December 8, 2023 Category: Biochemistry Authors: Yasemin İspirli Doğaç, Alfred Ngenge Tamfu, Selahattin Bozkurt, Mehmet Kayhan, Mustafa Teke, Ozgur Ceylan Tags: ORIGINAL ARTICLE Source Type: research

Analysis of highly frequent point mutations in glycoprotein C, glycoprotein N, and nucleoprotein of CCHFV
In conclusion, we observed that point mutations could impact structure, stability, and host–virus interaction of protein, and might lead to evolution of new strains for better survival and drug resistance. (Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - December 7, 2023 Category: Biochemistry Authors: Neha Kaushal, Manoj Baranwal Tags: ORIGINAL ARTICLE Source Type: research