Biotechnology and Applied Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Correlation between plasma PSGL ‐1 and FIGO stage, tumor metastasis, and survival in epithelial ovarian cancer
AbstractPlasma circulating P-selectin glycoprotein ligand-1 (PSGL-1) levels and its clinical correlation in patients with epithelial ovarian cancer (EOC) are unknown. The study determined plasma PSGL-1 levels in EOC patients and investigated its relationship with clinicopathological factors and prognosis. Plasma PSGL-1 levels were measured using ELISA in 69 patients with EOC, 34 patients with benign ovarian cystadenoma, and 36 healthy controls. Subsequently, the relationship between PSGL-1 levels and clinicopathological characteristics of patients, as well as the prognosis of EOC patients, was examined. Additionally, the s...
Source: Biotechnology and Applied Biochemistry - March 18, 2024 Category: Biochemistry Authors: WenHui Li, Cheng Fang, Ya Gao, Yan Gao, FengShang Yan, BiLiang Chen, MingJuan Xu Tags: ORIGINAL ARTICLE Source Type: research

Optimization of hematite nanoparticles from natural ore as novel imaging agents: A Green Chemistry approach
AbstractIn this research, we propose an environmentally friendly method for producing hematite nanoparticles (H-NPs) from natural hematite ore, focusing on their application as efficient contrast agents in x-ray and computed tomography (CT) imaging for medical purposes. The process involves the reduction of iron oxide within the ore to attain the desired hematite phase, crucial for synthesizing H-NPs. To ensure sustainability, we adopted a Green Chemistry approach, utilizing a combination of carbon soot and limestone for the purification process, thereby achieving eco-conscious production. The produced H-NPs were thoroughl...
Source: Biotechnology and Applied Biochemistry - March 15, 2024 Category: Biochemistry Authors: Albert Asha, Munusamy Chamundeeswari, Rayappan Mary Nancy Flora, Kuppusamy Ravichandran Preethy Tags: ORIGINAL ARTICLE Source Type: research

Accumulation and toxicity of biologically produced gold nanoparticles in different types of specialized mammalian cells
In this study, AuNPs were produced byFusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), energy dispersive x-ray spectroscopy (EDS), and Zetasizer. Obtained AuNPs were about 15  nm in size with a zeta potential of –35.8 mV. The AuNPs were added to cancer cells (4T1), noncancer cells (NIH/3T3), and macrophages (RAW264.7). The viability decreased in 4T1 (77 ± 3.74%) in contrast to NIH/3T3 and RAW264.7 cells (89 ± 4.9% and 90 ± 3.5%, respectively). The 4T1 cancer cells also showed the highest uptake and accumulation of Au (∼80% of AuNPs was internalized) as determined by grap...
Source: Biotechnology and Applied Biochemistry - March 14, 2024 Category: Biochemistry Authors: Parastoo Pourali, Milan Svoboda, Eva Neuh öferová, Volha Dzmitruk, Veronika Benson Tags: ORIGINAL ARTICLE Source Type: research

Pemetrexed loaded gold nanoparticles as cytotoxic and apoptosis inducers in lung cancer cells through ROS generation and mitochondrial dysfunction pathway
AbstractSupramolecular nanoparticles containing peptides and drugs have recently gained recognition as an effective tumor treatment drug delivery system. A multitarget drug termed pemetrexed is effective against various cancers, including nonsmall cell lung cancer. The work aims to establish the capability of pemetrexed gold nanoparticles (PEM-AuNPs) to induce apoptosis and explore molecular changes. X-ray diffraction, Fourier-transform infrared spectroscopy, ultraviolet –visible spectroscopy, scanning electron microscope, and transmission electron microscope were used to investigate the synthesized nanoparticles. The MT...
Source: Biotechnology and Applied Biochemistry - March 13, 2024 Category: Biochemistry Authors: Baby Shakila P, Tamilmani Periasamy, Abdullah A. Alarfaj, Palanisamy Arulselvan, Rajeswari Ravindran, Jagadeesh Suriyaprakash, Indumathi Thangavelu Tags: ORIGINAL ARTICLE Source Type: research

Development of a prognostic model for lung adenocarcinoma polarity ‐related genes and analysis of immune landscape
AbstractDespite the progress made in the management of lung adenocarcinoma (LUAD), the overall prognosis for LUAD individuals remains suboptimal. While the role of cell polarity in tumor invasion and metastasis is well established, its prognostic significance in LUAD is still unknown. Differential analysis was performed on the Cancer Genome Atlas (TCGA)-LUAD and normal lung tissue, and candidate genes were identified by intersecting differentially expressed genes with polarity-related genes (PRGs). A prognostic model was constructed using univariate and multivariate Cox regression and LASSO regression. To enhance the robus...
Source: Biotechnology and Applied Biochemistry - March 13, 2024 Category: Biochemistry Authors: Hongqiu Xu, Wenqiang Du, Xuelong Jing, Jingen Xie, Pengfei Li Tags: ORIGINAL ARTICLE Source Type: research

A novel graphene oxide ‐based fluorescence method for detection of urine glycosaminoglycans
In this study, a novel fluorometric method was developed to measure total GAGs in urine. Graphene oxide (GO) and rhodamine B (RhB), a cationic fluorescent dye, were employed in the development of the method. RhB attaches to the GO surface via electrostatic attraction, leading to the quenching of its fluorescence upon the establishment of the RhB –GO complex. However, the presence of GAGs prompts a resurgence of intense fluorescence. The linear range of the method is between 5.00 and 70.00 mg/L. The total GAG levels of urine samples analyzed using the method agree with the results of the biochemistry analysis laboratory ...
Source: Biotechnology and Applied Biochemistry - March 7, 2024 Category: Biochemistry Authors: Ceren Bak ır Kösoğlu, Süreyya Dede, Emine Karakuş, Ali Erdoğmuş, Bahadır Keskin, Hasan Önal Tags: ORIGINAL ARTICLE Source Type: research

Metabolite profiling of Piper longum L. fruit volatiles by two ‐dimensional gas chromatography and time‐of‐flight mass spectrometry: Insights into the chemical complexity
AbstractPiper longum L. (long pepper) is an economically and industrially important medicinal plant. However, the characterization of its volatiles has only been analyzed by gas chromatography –mass spectrometry (GC–MS). In the present study, precise characterization ofP. longum fruit volatiles has been performed for the first time through advanced two-dimensional gas chromatography –time-of-flight spectrometry (GC×GC–TOFMS). A total of 146 constituents accounting for 93.79% were identified, of which 30 were reported for the first time. All these constituents were classified into alcohols (4.5%), alkanes (8.9%), a...
Source: Biotechnology and Applied Biochemistry - March 6, 2024 Category: Biochemistry Authors: Manaswini Dash, Subhashree Singh, Suprava Sahoo, Malvika Dutt, Basudeba Kar Tags: ORIGINAL ARTICLE Source Type: research

Selenized lactic acid bacteria microencapsulated by spray drying: A promising strategy for beef cattle feed supplementation
This study aimed to assess the technical feasibility of incorporating selenizedLactobacillus spp. microencapsulated via spray drying into cattle feed. Gum Arabic and maltodextrin were used as encapsulating agents. The encapsulation process was carried out with a drying air flow rate of 1.75  m3/min, inlet air temperature of 90 °C, and outlet air temperature of 75°C. The viability of the encapsulated microorganisms and the technological characteristics of the obtained microparticles were evaluated. Microorganisms were incorporated into beef cattle feed to supplement their diet with up to 0.3 mg of Se per kilogram of fee...
Source: Biotechnology and Applied Biochemistry - March 5, 2024 Category: Biochemistry Authors: Guilherme Schwingel Henn, Larissa Zago Cima, Beatriz Fabris Bettanin, Cl áudia Schlabitz, Daniel Neutzling Lehn, Claucia Fernanda Volken de Souza, Lucélia Hoehne Tags: ORIGINAL ARTICLE Source Type: research