Mechanisms for the Enhancement of Caproic Acid and H < sub > 2 < /sub > Production in Ruminococcaceae Bacterium CPB6 by Fe(II) and Mg(II): Growth and Gene Transcription Analyses
Appl Biochem Biotechnol. 2024 May 2. doi: 10.1007/s12010-024-04920-8. Online ahead of print.ABSTRACTThe production of caproic acid (CA) and hydrogen gas (H2) from organic wastewater is economically attractive. The Ruminococcaceae bacterium CPB6 has demonstrated potential for CA production from lactate-containing wastewater. However, our understanding of the effects of Fe2+ and Mg2+ on the growth and metabolism of strain CPB6 remains limited. Therefore, this study aims to investigate the impact of Fe2+ and Mg2+ on CA and H2 production, as well as on the expression of key genes involved in CA and H2 biosynthesis pathway. The...
Source: Applied Biochemistry and Biotechnology - May 2, 2024 Category: Biochemistry Authors: Guihong Xie Duo Huang Xuemei Duan Jun Liu Siqi Yuan Yong Tao Source Type: research
Recent Progress in the Application of Tau Protein Biosensors for Diagnosis of Neurodegenerative Diseases
Appl Biochem Biotechnol. 2024 May 2. doi: 10.1007/s12010-024-04960-0. Online ahead of print.ABSTRACTThe microtubule-associated Tau protein is found in the central nervous system (CNS) in six major isoforms. Neurodegenerative diseases have been linked to post-translational changes of Tau, most notably phosphorylation. Tau protein's molecular diversity is highly helpful in the identification of neurodegenerative illnesses. Nonetheless, one major obstacle to the early detection of brain illness is the nanoscale identification of tau proteins. The standard methods for identifying tau protein include western blotting, polymeras...
Source: Applied Biochemistry and Biotechnology - May 2, 2024 Category: Biochemistry Authors: Sophia Nazir Rabail Azhar Iqbal Source Type: research
Optimization of Enzymatic Transesterification of Acid Oil for Biodiesel Production Using a Low-Cost Lipase: The Effect of Transesterification Conditions and the Synergy of Lipases with Different Regioselectivity
This study describes the enzymatic production of second-generation biodiesel using low-quality acid oil as a substrate. Biolipasa-R, a commercially available and low-cost lipase, was employed for enzymatic transesterification. Response surface methodology was applied to optimize the enzymatic transesterification process. The optimal conditions for biodiesel production, which comprised 42% lipase concentration (per weight of oil), 32% water content (per weight of oil), a methanol to oil molar ratio of 3:1, pH 7.0 and reaction temperature 30°C, resulted in the highest fatty acid methyl ester (FAME) content (71.3%). Subseque...
Source: Applied Biochemistry and Biotechnology - May 2, 2024 Category: Biochemistry Authors: Alexandra Moschona Androniki Spanou Ioannis V Pavlidis Anastasios J Karabelas Sotiris I Patsios Source Type: research
Green synthesized silver nanoparticles of < em > Terminalia bellirica < /em > leaves extract: synthesis, characterization, < em > in-silico < /em > studies, and antimalarial activity
In conclusion, this novel class of AgNPs may be used as a potential therapeutic replacement for the treatment of malaria.PMID:38696111 | DOI:10.1080/21691401.2024.2339429 (Source: Artificial Cells, Nanomedicine and Biotechnology)
Source: Artificial Cells, Nanomedicine and Biotechnology - May 2, 2024 Category: Biotechnology Authors: Sujeet Singh Hemant Arya Welka Sahu K Sony Reddy Surendra Nimesh Bader Saud Alotaibi Mohammed Ageeli Hakami Hassan H Almasoudi Khater Balatone Gezira Hessien Mohammad Raghibul Hasan Summya Rashid Tarun Kumar Bhatt Source Type: research
Targeted photodynamic therapy technique of Janus nanoparticles on breast cancer
Artif Cells Nanomed Biotechnol. 2024 Dec;52(1):270-277. doi: 10.1080/21691401.2024.2347369. Epub 2024 May 2.ABSTRACTSpherical gold/polyacrylic acid (Au/PAA) polymer-inorganic Janus nanoparticles (JNPs) with simultaneous therapeutic and targeting functions were fabricated. The obtained Au/PAA JNPs were further selectively functionalized with folic acid (FA) and thiol PEG amine (SH-PEG-NH2) on Au sides to provide superior biocompatibility and active targeting, while the other PAA sides were loaded with 5-aminolevulinic acid (5-ALA) to serve as a photosensitizer (PS) for photodynamic therapeutic (PDT) effects on MCF-7 cancer ...
Source: Artificial Cells, Nanomedicine and Biotechnology - May 2, 2024 Category: Biotechnology Authors: Hanieh Montaseri Heidi Abrahamse Source Type: research
Antibacterial potential of < em > Euphorbia canariensis < /em > against < em > Pseudomonas aeruginosa < /em > bacteria causing respiratory tract infections
Artif Cells Nanomed Biotechnol. 2024 Dec;52(1):261-269. doi: 10.1080/21691401.2024.2345891. Epub 2024 May 2.ABSTRACTThe widespread dissemination of bacterial resistance has led to great attention being paid to finding substitutes for traditionally used antibiotics. Plants are rich in various phytochemicals that could be used as antibacterial therapies. Here, we elucidate the phytochemical profile of Euphorbia canariensis ethanol extract (EMEE) and then elucidate the antibacterial potential of ECEE against Pseudomonas aeruginosa clinical isolates. ECEE showed minimum inhibitory concentrations ranging from 128 to 512 µg/mL....
Source: Artificial Cells, Nanomedicine and Biotechnology - May 2, 2024 Category: Biotechnology Authors: Badriyah Alotaibi Engy Elekhnawy Thanaa A El-Masry Asmaa Saleh Manal E Alosaimi Khalid Nijr Alotaibi Walaa A Negm Source Type: research
Sensing patches for biomarker identification in skin-derived biofluids
Biosens Bioelectron. 2024 Apr 22;258:116326. doi: 10.1016/j.bios.2024.116326. Online ahead of print.ABSTRACTIn conventional clinical disease diagnosis and screening based on biomarker detection, most analysis samples are collected from serum, blood. However, these invasive collection methods require specific instruments, professionals, and may lead to infection risks. Additionally, the diagnosis process suffers from untimely results. The identification of skin-related biomarkers plays an unprecedented role in early disease diagnosis. More importantly, these skin-mediated approaches for collecting biomarker-containing biofl...
Source: Biosensors and Bioelectronics - May 2, 2024 Category: Biotechnology Authors: Chen Wei Danni Fu Tianyue Ma Mo Chen Fangling Wang Guojun Chen Zejun Wang Source Type: research
Development of a thermotaxis and rheotaxis microfluidic device for motile spermatozoa sorting
This study introduces a groundbreaking development in the form of a thermotaxis and rheotaxis microfluidic (TRMC) device designed for efficient motile spermatozoa sorting within a short 15-min timeframe. The TRMC device mimics the natural sperm sorting mechanism of the oviduct, selecting spermatozoa with superior motility and DNA integrity. The experimental outcomes demonstrate a remarkable enhancement in the percentage of progressive spermatozoa following sorting, soaring from 3.90% to an impressive 96.11% when subjected to a temperature decrease from 38 °C to 35 °C. Notably, sperm motility exhibited a substantial 69% i...
Source: Biosensors and Bioelectronics - May 2, 2024 Category: Biotechnology Authors: Teng-Kuan Huang Chung-Hsien Huang Pei-An Chen Ching Hung Chen Farn Lu Wen-Ju Yang Jack Yu Jen Huang Bor-Ran Li Source Type: research
A "dual-key-and-lock" platform for distinguishing autophagy during neuroinflammation
Biosens Bioelectron. 2024 Apr 27;258:116344. doi: 10.1016/j.bios.2024.116344. Online ahead of print.ABSTRACTAutophagy is an essential degradative process that governs the renewal of organelle and maintains the homeostasis of cellular microenvironment. Its dysregulation has been demonstrated to be an indicator for neuroinflammation. To elucidate the interrelationship between neuroinflammation and autophagy, optical probes are ideal tools as they offer a number of advantages such as high spatiotemporal resolution and non-invasive sensing, which help to visualize the physiological and pathological functions of interested anal...
Source: Biosensors and Bioelectronics - May 2, 2024 Category: Biotechnology Authors: Shuyang Zhai Wei Hu Zhihong Liu Yingbin Liu Source Type: research
Analysis of the molecular basis for the non-amylolytic and non-proteolytic nature of Aspergillus vadensis CBS 113365
N Biotechnol. 2024 Apr 30:S1871-6784(24)00014-1. doi: 10.1016/j.nbt.2024.04.003. Online ahead of print.ABSTRACTAspergillus vadensis CBS 113365, a close relative of A. niger, has been suggested as a more favourable alternative for recombinant protein production as it does not acidify the culture medium and produces very low levels of extracellular proteases. The aim of this study was to investigate the underlying cause of the non-amylolytic and non-proteolytic phenotype of A. vadensis CBS 113365. Our results demonstrate that the non-functionality of the amylolytic transcription factor AmyR in A. vadensis CBS 113365 is prima...
Source: New Biotechnology - May 2, 2024 Category: Biotechnology Authors: Dujuan Liu Sandra Garrigues Helena Culleton Vincent A McKie Ronald P de Vries Source Type: research
Smoothed Nested Testing on Directed Acyclic Graphs
Biometrika. 2022 Jun;109(2):457-471. doi: 10.1093/biomet/asab041. Epub 2021 Jul 2.ABSTRACTWe consider the problem of multiple hypothesis testing when there is a logical nested structure to the hypotheses. When one hypothesis is nested inside another, the outer hypothesis must be false if the inner hypothesis is false. We model the nested structure as a directed acyclic graph, including chain and tree graphs as special cases. Each node in the graph is a hypothesis and rejecting a node requires also rejecting all of its ancestors. We propose a general framework for adjusting node-level test statistics using the known logical...
Source: Biometrika - May 2, 2024 Category: Biotechnology Authors: J H Loper L Lei W Fithian W Tansey Source Type: research
Advancing the construction and application of genome-scale metabolic models for oleaginous microorganisms
Bioresour Technol. 2024 Apr 30:130706. doi: 10.1016/j.biortech.2024.130706. Online ahead of print.NO ABSTRACTPMID:38697368 | DOI:10.1016/j.biortech.2024.130706 (Source: Bioresource Technology)
Source: Bioresource Technology - May 2, 2024 Category: Biotechnology Authors: Chao Ye Source Type: research
Integration of anaerobic digestion and electrodialysis for methane yield promotion and in-situ ammonium recovery
This study proposed a novel approach, the electrodialysis-integrated AD (ADED) system, for in-situ recovery of ammonium (NH4+) while simultaneously enhancing AD performance. The ADED reactor was operated at two different NH4+-N concentrations (5,000 mg/L and 10,000 mg/L) to evaluate its performance against a conventional AD reactor. The results indicate that the ADED technology effectively reduced the NH4+-N concentration to below 2,000 mg/L, achieving this with a competitive energy consumption. Moreover, the ADED reactor demonstrated a 1.43-fold improvement in methane production when the influent NH4+-N was 5,000 mg/L, an...
Source: Bioresource Technology - May 2, 2024 Category: Biotechnology Authors: Jizhong Meng Lin Shi Yuansheng Hu Zhongzhong Wang Zhenhu Hu Xinmin Zhan Source Type: research
Propitious catalytic response of immobilized α-amylase from G. thermoleovorans in modified APTES-Fe < sub > 3 < /sub > O < sub > 4 < /sub > NPs for industrial bio-processing
Int J Biol Macromol. 2024 Apr 30:132021. doi: 10.1016/j.ijbiomac.2024.132021. Online ahead of print.ABSTRACTChallenges in enzyme and product recovery are currently intriguing in modern biotechnology. Coping enzyme stability, shelf life and efficiency, nanomaterials-based immobilization were epitomized of industrial practice. Herein, a α-amylase from Geobacillus thermoleovorans was purified and bound effectively on to a modified 3-Aminopropyltriethoxysilane (APTES)-Fe3O4 nanoparticle. It was revealed that the carrier-bound enzyme catalysis (pH 8 and 60 °C) was significant in contrast to the free enzyme (pH 7.5 and 55 °C)...
Source: International Journal of Biological Macromolecules - May 2, 2024 Category: Biochemistry Authors: Kotresh Kyathanahalli Rajashekar Avinash Basavarajappa Shivayogeeswar Eshwrappa Neelagund Gurumurthy Dummi Mahadevan Rajeshwara Achur Prabhanshu Kumar Source Type: research
Establishment of genome-editing system and assembly of a near-complete genome in broomcorn millet
J Integr Plant Biol. 2024 May 2. doi: 10.1111/jipb.13664. Online ahead of print.ABSTRACTThe ancient crop broomcorn millet (Panicum miliaceum L.) is an indispensable orphan crop in semi-arid regions due to its short life cycle and excellent abiotic stress tolerance. These advantages make it an important alternative crop to increase food security and achieve the goal of zero hunger, particularly in light of the uncertainty of global climate change. However, functional genomic and biotechnological research in broomcorn millet has been hampered due to a lack of genetic tools such as transformation and genome-editing techniques...
Source: Journal of Integrative Plant Biology - May 2, 2024 Category: Biology Authors: Yang Liu Zixiang Cheng Weiyao Chen Chuanyin Wu Jinfeng Chen Yi Sui Source Type: research
