Identification and characterization of camptothecin tailoring enzymes in Nothapodytes tomentosa
J Integr Plant Biol. 2024 Mar 22. doi: 10.1111/jipb.13649. Online ahead of print.ABSTRACTCamptothecin is a complex monoterpenoid indole alkaloid with remarkable antitumor activity. Given that two C-10 modified camptothecin derivatives, topotecan and irinotecan, have been approved as potent anticancer agents, there is a critical need for methods to access other aromatic ring-functionalized congeners (e.g., C-9, C-10, etc.). However, contemporary methods for chemical oxidation are generally harsh and low-yielding when applied to the camptothecin scaffold, thereby limiting the development of modified derivatives. Reported her...
Source: Journal of Integrative Plant Biology - March 22, 2024 Category: Biology Authors: Yin Chen Jian-Ping Huang Yong-Jiang Wang Meng-Ling Tu Junheng Li Bingyan Xu Guoqing Peng Jing Yang Sheng-Xiong Huang Source Type: research

The transcriptional control of LcIDL1-LcHSL2 complex by LcARF5 integrates auxin and ethylene signaling for litchi fruitlet abscission
J Integr Plant Biol. 2024 Mar 22. doi: 10.1111/jipb.13646. Online ahead of print.ABSTRACTAt the physiological level, the interplay between auxin and ethylene has long been recognized as crucial for the regulation of organ abscission in plants. However, the underlying molecular mechanisms remain unknown. Here, we identified transcription factors involved in indoleacetic acid (IAA) and ethylene (ET) signaling that directly regulate the expression of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) and its receptor HAESA (HAE), which are key components initiating abscission. Specifically, litchi IDA-like 1 (LcIDL1) interacts with ...
Source: Journal of Integrative Plant Biology - March 22, 2024 Category: Biology Authors: Xingshuai Ma Zidi He Ye Yuan Zhijian Liang Hang Zhang Vilde Olsson Lalun Zhuoyi Liu Yanqing Zhang Zhiqiang Huang Yulian Huang Jianguo Li Minglei Zhao Source Type: research

Identification and characterization of camptothecin tailoring enzymes in Nothapodytes tomentosa
J Integr Plant Biol. 2024 Mar 22. doi: 10.1111/jipb.13649. Online ahead of print.ABSTRACTCamptothecin is a complex monoterpenoid indole alkaloid with remarkable antitumor activity. Given that two C-10 modified camptothecin derivatives, topotecan and irinotecan, have been approved as potent anticancer agents, there is a critical need for methods to access other aromatic ring-functionalized congeners (e.g., C-9, C-10, etc.). However, contemporary methods for chemical oxidation are generally harsh and low-yielding when applied to the camptothecin scaffold, thereby limiting the development of modified derivatives. Reported her...
Source: Journal of Integrative Plant Biology - March 22, 2024 Category: Biology Authors: Yin Chen Jian-Ping Huang Yong-Jiang Wang Meng-Ling Tu Junheng Li Bingyan Xu Guoqing Peng Jing Yang Sheng-Xiong Huang Source Type: research

The transcriptional control of LcIDL1-LcHSL2 complex by LcARF5 integrates auxin and ethylene signaling for litchi fruitlet abscission
J Integr Plant Biol. 2024 Mar 22. doi: 10.1111/jipb.13646. Online ahead of print.ABSTRACTAt the physiological level, the interplay between auxin and ethylene has long been recognized as crucial for the regulation of organ abscission in plants. However, the underlying molecular mechanisms remain unknown. Here, we identified transcription factors involved in indoleacetic acid (IAA) and ethylene (ET) signaling that directly regulate the expression of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) and its receptor HAESA (HAE), which are key components initiating abscission. Specifically, litchi IDA-like 1 (LcIDL1) interacts with ...
Source: Journal of Integrative Plant Biology - March 22, 2024 Category: Biology Authors: Xingshuai Ma Zidi He Ye Yuan Zhijian Liang Hang Zhang Vilde Olsson Lalun Zhuoyi Liu Yanqing Zhang Zhiqiang Huang Yulian Huang Jianguo Li Minglei Zhao Source Type: research

Identification and characterization of camptothecin tailoring enzymes in Nothapodytes tomentosa
J Integr Plant Biol. 2024 Mar 22. doi: 10.1111/jipb.13649. Online ahead of print.ABSTRACTCamptothecin is a complex monoterpenoid indole alkaloid with remarkable antitumor activity. Given that two C-10 modified camptothecin derivatives, topotecan and irinotecan, have been approved as potent anticancer agents, there is a critical need for methods to access other aromatic ring-functionalized congeners (e.g., C-9, C-10, etc.). However, contemporary methods for chemical oxidation are generally harsh and low-yielding when applied to the camptothecin scaffold, thereby limiting the development of modified derivatives. Reported her...
Source: Journal of Integrative Plant Biology - March 22, 2024 Category: Biology Authors: Yin Chen Jian-Ping Huang Yong-Jiang Wang Meng-Ling Tu Junheng Li Bingyan Xu Guoqing Peng Jing Yang Sheng-Xiong Huang Source Type: research

The transcriptional control of LcIDL1-LcHSL2 complex by LcARF5 integrates auxin and ethylene signaling for litchi fruitlet abscission
J Integr Plant Biol. 2024 Mar 22. doi: 10.1111/jipb.13646. Online ahead of print.ABSTRACTAt the physiological level, the interplay between auxin and ethylene has long been recognized as crucial for the regulation of organ abscission in plants. However, the underlying molecular mechanisms remain unknown. Here, we identified transcription factors involved in indoleacetic acid (IAA) and ethylene (ET) signaling that directly regulate the expression of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) and its receptor HAESA (HAE), which are key components initiating abscission. Specifically, litchi IDA-like 1 (LcIDL1) interacts with ...
Source: Journal of Integrative Plant Biology - March 22, 2024 Category: Biology Authors: Xingshuai Ma Zidi He Ye Yuan Zhijian Liang Hang Zhang Vilde Olsson Lalun Zhuoyi Liu Yanqing Zhang Zhiqiang Huang Yulian Huang Jianguo Li Minglei Zhao Source Type: research

Identification and characterization of camptothecin tailoring enzymes in Nothapodytes tomentosa
J Integr Plant Biol. 2024 Mar 22. doi: 10.1111/jipb.13649. Online ahead of print.ABSTRACTCamptothecin is a complex monoterpenoid indole alkaloid with remarkable antitumor activity. Given that two C-10 modified camptothecin derivatives, topotecan and irinotecan, have been approved as potent anticancer agents, there is a critical need for methods to access other aromatic ring-functionalized congeners (e.g., C-9, C-10, etc.). However, contemporary methods for chemical oxidation are generally harsh and low-yielding when applied to the camptothecin scaffold, thereby limiting the development of modified derivatives. Reported her...
Source: Journal of Integrative Plant Biology - March 22, 2024 Category: Biology Authors: Yin Chen Jian-Ping Huang Yong-Jiang Wang Meng-Ling Tu Junheng Li Bingyan Xu Guoqing Peng Jing Yang Sheng-Xiong Huang Source Type: research

The transcriptional control of LcIDL1-LcHSL2 complex by LcARF5 integrates auxin and ethylene signaling for litchi fruitlet abscission
J Integr Plant Biol. 2024 Mar 22. doi: 10.1111/jipb.13646. Online ahead of print.ABSTRACTAt the physiological level, the interplay between auxin and ethylene has long been recognized as crucial for the regulation of organ abscission in plants. However, the underlying molecular mechanisms remain unknown. Here, we identified transcription factors involved in indoleacetic acid (IAA) and ethylene (ET) signaling that directly regulate the expression of INFLORESCENCE DEFICIENT IN ABSCISSION (IDA) and its receptor HAESA (HAE), which are key components initiating abscission. Specifically, litchi IDA-like 1 (LcIDL1) interacts with ...
Source: Journal of Integrative Plant Biology - March 22, 2024 Category: Biology Authors: Xingshuai Ma Zidi He Ye Yuan Zhijian Liang Hang Zhang Vilde Olsson Lalun Zhuoyi Liu Yanqing Zhang Zhiqiang Huang Yulian Huang Jianguo Li Minglei Zhao Source Type: research

UVR8-TCP4-LOX2 module regulates UV-B tolerance in Arabidopsis
In this study, we demonstrate that the UV RESISTANCE LOCUS 8 - TEOSINTE BRANCHED1, Cycloidea and PCF 4 - LIPOXYGENASE2 (UVR8-TCP4-LOX2) module regulates UV-B tolerance dependent on JA signaling pathway in Arabidopsis thaliana. We show that the nucleus-localized UVR8 physically interacts with TCP4 to increase the DNA-binding activity of TCP4 and upregulate the JA biosynthesis gene LOX2. Furthermore, UVR8 activates the expression of LOX2 in a TCP4-dependent manner. Our genetic analysis also provides evidence that TCP4 acts downstream of UVR8 and upstream of LOX2 to mediate plant responses to UV-B stress. Our results illustra...
Source: Journal of Integrative Plant Biology - March 20, 2024 Category: Biology Authors: Cheng Li Jiancan Du Huini Xu Zhenhua Feng Caspar C C Chater Yuanwen Duan Yongping Yang Xudong Sun Source Type: research

UVR8-TCP4-LOX2 module regulates UV-B tolerance in Arabidopsis
In this study, we demonstrate that the UV RESISTANCE LOCUS 8 - TEOSINTE BRANCHED1, Cycloidea and PCF 4 - LIPOXYGENASE2 (UVR8-TCP4-LOX2) module regulates UV-B tolerance dependent on JA signaling pathway in Arabidopsis thaliana. We show that the nucleus-localized UVR8 physically interacts with TCP4 to increase the DNA-binding activity of TCP4 and upregulate the JA biosynthesis gene LOX2. Furthermore, UVR8 activates the expression of LOX2 in a TCP4-dependent manner. Our genetic analysis also provides evidence that TCP4 acts downstream of UVR8 and upstream of LOX2 to mediate plant responses to UV-B stress. Our results illustra...
Source: Journal of Integrative Plant Biology - March 20, 2024 Category: Biology Authors: Cheng Li Jiancan Du Huini Xu Zhenhua Feng Caspar C C Chater Yuanwen Duan Yongping Yang Xudong Sun Source Type: research

ACBP4-WRKY70-RAP2.12 module positively regulates submergence-induced hypoxia response in Arabidopsis thaliana
J Integr Plant Biol. 2024 Mar 19. doi: 10.1111/jipb.13647. Online ahead of print.ABSTRACTACYL-CoA-BINDING PROTEINs (ACBPs) play crucial regulatory roles during plant response to hypoxia, but their molecular mechanisms remain poorly understood. Our study reveals that ACBP4 serves as a positive regulator of the plant hypoxia response by interacting with WRKY70, influencing its nucleocytoplasmic shuttling in Arabidopsis thaliana. Furthermore, we demonstrate the direct binding of WRKY70 to the ACBP4 promoter, resulting in its upregulation and suggesting a positive feedback loop. Additionally, we pinpointed a phosphorylation si...
Source: Journal of Integrative Plant Biology - March 19, 2024 Category: Biology Authors: Mengyun Guo Yingjun Yao Kangqun Yin Luna Tan Meng Liu Jing Hou Han Zhang Ruyun Liang Xinran Zhang Heng Yang Xiaoxiao Chen Jinrui Tan Yan Song Shangling Lou Liyang Chen Xuejing Liu Si Tang Yongqi Hu Jin Yan Wensen Fu Kai Yang Ruijia Zhang Xuerui Li Yao Liu Source Type: research

FKF1b controls reproductive transition associated with adaptation to geographical distribution in maize
In this study, we identified two FKF1 homologs in the maize genome, named ZmFKF1a and ZmFKF1b, and indicated that ZmFKF1a and ZmFKF1b independently regulate reproductive transition through interacting with ZmCONZ1 and ZmGI1 to increase the transcription levels of ZmCONZ1 and ZCN8. We demonstrated that ZmFKF1b underwent artificial selection during modern breeding in China probably due to its role in geographical adaptation. Furthermore, our data suggested that ZmFKF1bHap_C7 may be an elite allele, which increases the abundance of ZmCONZ1 mRNA more efficiently and adapt to a wider range of temperature zone than that of ZmFKF...
Source: Journal of Integrative Plant Biology - March 19, 2024 Category: Biology Authors: Suhui Chen Shan Gao Dongyang Wang Jie Liu Yingying Ren Zhihan Wang Xin Wei Qin Wang Xuehui Huang Source Type: research

A converged ubiquitin-proteasome pathway for the degradation of TOC and TOM tail-anchored receptors
J Integr Plant Biol. 2024 Mar 19. doi: 10.1111/jipb.13645. Online ahead of print.ABSTRACTIn plants, thousands of nucleus-encoded proteins translated in the cytosol are sorted to chloroplasts and mitochondria by binding to specific receptors of the TOC (translocon on the outer chloroplast membrane) and the TOM (translocon on the outer mitochondrial membrane) complexes for import into those organelles. The degradation pathways for these receptors are unclear. Here, we discovered a converged ubiquitin-proteasome pathway for the degradation of Arabidopsis thaliana TOC and TOM tail-anchored receptors. The receptors are ubiquiti...
Source: Journal of Integrative Plant Biology - March 19, 2024 Category: Biology Authors: Meijing Yang Shuai Chen Shey-Li Lim Lang Yang Jia Yi Zhong Koon Chuen Chan Zhizhu Zhao Kam-Bo Wong Junqi Wang Boon Leong Lim Source Type: research

ACBP4-WRKY70-RAP2.12 module positively regulates submergence-induced hypoxia response in Arabidopsis thaliana
J Integr Plant Biol. 2024 Mar 19. doi: 10.1111/jipb.13647. Online ahead of print.ABSTRACTACYL-CoA-BINDING PROTEINs (ACBPs) play crucial regulatory roles during plant response to hypoxia, but their molecular mechanisms remain poorly understood. Our study reveals that ACBP4 serves as a positive regulator of the plant hypoxia response by interacting with WRKY70, influencing its nucleocytoplasmic shuttling in Arabidopsis thaliana. Furthermore, we demonstrate the direct binding of WRKY70 to the ACBP4 promoter, resulting in its upregulation and suggesting a positive feedback loop. Additionally, we pinpointed a phosphorylation si...
Source: Journal of Integrative Plant Biology - March 19, 2024 Category: Biology Authors: Mengyun Guo Yingjun Yao Kangqun Yin Luna Tan Meng Liu Jing Hou Han Zhang Ruyun Liang Xinran Zhang Heng Yang Xiaoxiao Chen Jinrui Tan Yan Song Shangling Lou Liyang Chen Xuejing Liu Si Tang Yongqi Hu Jin Yan Wensen Fu Kai Yang Ruijia Zhang Xuerui Li Yao Liu Source Type: research

FKF1b controls reproductive transition associated with adaptation to geographical distribution in maize
In this study, we identified two FKF1 homologs in the maize genome, named ZmFKF1a and ZmFKF1b, and indicated that ZmFKF1a and ZmFKF1b independently regulate reproductive transition through interacting with ZmCONZ1 and ZmGI1 to increase the transcription levels of ZmCONZ1 and ZCN8. We demonstrated that ZmFKF1b underwent artificial selection during modern breeding in China probably due to its role in geographical adaptation. Furthermore, our data suggested that ZmFKF1bHap_C7 may be an elite allele, which increases the abundance of ZmCONZ1 mRNA more efficiently and adapt to a wider range of temperature zone than that of ZmFKF...
Source: Journal of Integrative Plant Biology - March 19, 2024 Category: Biology Authors: Suhui Chen Shan Gao Dongyang Wang Jie Liu Yingying Ren Zhihan Wang Xin Wei Qin Wang Xuehui Huang Source Type: research