Aptamer-modified paper-based analytical devices for the detection of food hazards: Emerging applications and future perspective
Biotechnol Adv. 2024 Apr 29:108368. doi: 10.1016/j.biotechadv.2024.108368. Online ahead of print.ABSTRACTFood analysis plays a critical role in assessing human health risks and monitoring food quality and safety. Currently, there is a pressing need for a reliable, portable, and quick recognition element for point-of-care testing (POCT) to better serve the demands of on-site food analysis. Aptamer-modified paper-based analytical devices (Apt-PADs) have excellent characteristics of high portability, high sensitivity, high specificity, and on-site detection, which have been widely used and concerned in the field of food safet...
Source: Biotechnology Advances - May 1, 2024 Category: Biotechnology Authors: Mingwei Qin Imran Mahmood Khan Ning Ding Shuo Qi Xiaoze Dong Yin Zhang Zhouping Wang Source Type: research
Unravelling the essential elements for recombinant adeno-associated virus (rAAV) production in animal cell-based platforms
Biotechnol Adv. 2024 Apr 29;73:108370. doi: 10.1016/j.biotechadv.2024.108370. Online ahead of print.ABSTRACTRecombinant adeno-associated viruses (rAAVs) stand at the forefront of gene therapy applications, holding immense significance for their safe and efficient gene delivery capabilities. The constantly increasing and unmet demand for rAAVs underscores the need for a more comprehensive understanding of AAV biology and its impact on rAAV production. In this literature review, we delved into AAV biology and rAAV manufacturing bioprocesses, unravelling the functions and essentiality of proteins involved in rAAV production. ...
Source: Biotechnology Advances - May 1, 2024 Category: Biotechnology Authors: David Catal án-Tatjer Konstantina Tzimou Lars K Nielsen Jes ús Lavado-García Source Type: research
Reproductive Characteristics and Suitability of Sterile dead end Knockout Nibe Croaker as a Recipient for Intraperitoneal Germ Cell Transplantation
In this study, nibe croaker was utilized as a model for marine teleosts that produce small pelagic eggs, and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (CRISPR/Cas9) system was utilized to produce dnd KO fish. The F1 generation, which carried a nonsense mutation in the dnd gene, was produced by mating founder individuals with wild-type counterparts. Subsequently, the F2 generation was produced by mating the resulting males and females. Among the F2 generations, 24.0% consisted of homozygous KO individuals. Histological analysis revealed that primordial germ cells (PGCs...
Source: Marine Biotechnology - May 1, 2024 Category: Biotechnology Authors: Ryosuke Yazawa Kyoichiro Saitoh Akihiro Yamauchi Onur Ey üboğlu Kana Ozawa Wataru Kawamura Tetsuro Morita Yutaka Takeuchi Goro Yoshizaki Source Type: research
A Novel Thermo-Alkaline Stable GDSL/SGNH Esterase with Broad Substrate Specificity from a Deep-Sea Pseudomonas sp
In this study, we report a new esterase (EstGoM) from a marine Pseudomonas sp. isolated at a depth of 1000 m in the Gulf of Mexico. Bioinformatic analyses revealed that EstGoM is an autotransporter esterase (type Va) and belongs to the lipolytic family II, forming a new subgroup. The purified recombinant EstGoM, with a molecular mass of 67.4 kDa, showed the highest hydrolytic activity with p-nitrophenyl octanoate (p-NP C8), although it was also active against p-NP C4, C5, C10, and C12. The optimum pH and temperature for EstGoM were 9 and 60 °C, respectively, but it retained more than 50% of its activity over the pH range ...
Source: Marine Biotechnology - May 1, 2024 Category: Biotechnology Authors: Jos é Luis Rodríguez-Mejía Itzel Anah í Hidalgo-Manzano Luis Felipe Muriel-Mill án Nancy Rivera-Gomez Diana X Sahonero-Canavesi Edmundo Castillo Liliana Pardo-L ópez Source Type: research
Research Progress on Strategies for Improving the Enzyme Properties of Bacteriophage Endolysins
J Microbiol Biotechnol. 2024 Feb 28;34(6):1-8. doi: 10.4014/jmb.2312.12050. Online ahead of print.ABSTRACTBacterial resistance to commonly used antibiotics is one of the major challenges to be solved today. Bacteriophage endolysins (Lysins) have become a hot research topic as a new class of antibacterial agents. They have promising applications in bacterial infection prevention and control in multiple fields, such as livestock and poultry farming, food safety, clinical medicine and pathogen detection. However, many phage endolysins display low bactericidal activities, short half-life and narrow lytic spectrums. Therefore, ...
Source: Journal of Microbiology and Biotechnology - May 1, 2024 Category: Biotechnology Authors: Yulu Wang Xue Wang Xin Liu Bokun Lin Source Type: research
Novel Qnr Families as Conserved and Intrinsic Quinolone Resistance Determinants in < em > Aeromonas < /em > spp
In this study, five environmental strains of Aeromonas spp. exhibiting multidrug resistance (MDR) were selected for whole-genome sequencing to ascertain their taxonomic assignment at the species-level and to delineate their ARG repertoires. Analyses of their genomes revealed the presence of one protein almost identical to AhQnr (A. hydrophila Qnr protein) and four novel proteins similar to AhQnr. To scrutinize the classification and taxonomic distribution of these proteins, all Aeromonas genomes deposited in the NCBI RefSeq genome database (1,222 genomes) were investigated. This revealed that these Aeromonas Qnr (AQnr) pro...
Source: Journal of Microbiology and Biotechnology - May 1, 2024 Category: Biotechnology Authors: Sang-Gyu Kim Bo-Eun Kim Jung Hun Lee Dae-Wi Kim Source Type: research
Evaluating the Nutritional Composition of Unripe Citrus and Its Effect on Inhibiting Adipogenesis and Adipocyte Differentiation
In this study, we assessed the nutritional components of unripe citrus peel and pressed juices, as well as their anti-obesity potential through the modulation of adipocyte differentiation and the expression of adipogenesisrelated genes, specifically PPARγ and C/EBPα, in 3T3-L1 preadipocytes. Our analysis revealed that unripe citrus peel exhibited elevated levels of fiber and protein compared to pressed juice, with markedly low levels of free sugar, particularly sucrose. The content of hesperidin, a representative flavonoid in citrus fruits, was 3,157.6 mg/kg in unripe citrus peel and 455.5 mg/kg in pressed juice, indicat...
Source: Journal of Microbiology and Biotechnology - May 1, 2024 Category: Biotechnology Authors: Sunghee Kim Eunbi Lee Juhye Park Ju-Ock Nam Soo Rin Kim Source Type: research
Morphological Engineering of Filamentous Fungi: Research Progress and Perspectives
J Microbiol Biotechnol. 2024 Mar 26;34(6):1-9. doi: 10.4014/jmb.2402.02007. Online ahead of print.ABSTRACTFilamentous fungi are important cell factories for the production of high-value enzymes and chemicals for the food, chemical, and pharmaceutical industries. Under submerged fermentation, filamentous fungi exhibit diverse fungal morphologies that are influenced by environmental factors, which in turn affect the rheological properties and mass transfer of the fermentation system, and ultimately the synthesis of products. In this review, we first summarize the mechanisms of mycelial morphogenesis and then provide an overv...
Source: Journal of Microbiology and Biotechnology - May 1, 2024 Category: Biotechnology Authors: Zhengwu Lu Zhiqun Chen Yunguo Liu Xuexue Hua Cuijuan Gao Jingjing Liu Source Type: research
How successful are AI-discovered drugs in clinical trials? A first analysis and emerging lessons
Drug Discov Today. 2024 Apr 29:104009. doi: 10.1016/j.drudis.2024.104009. Online ahead of print.ABSTRACTAI techniques are making inroads into the field of drug discovery. As a result, a growing number of drugs and vaccines have been discovered using AI. However, questions remain about the success of these molecules in clinical trials. To address these questions, we conducted a first analysis of the clinical pipelines of AI-native Biotech companies. In Phase I we find AI-discovered molecules have an 80-90% success rate, substantially higher than historic industry averages. This suggests, we argue, that AI is highly capable ...
Source: Drug Discovery Today - May 1, 2024 Category: Drugs & Pharmacology Authors: Madura Kp Jayatunga Margaret Ayers Lotte Bruens Dhruv Jayanth Christoph Meier Source Type: research
Homochiral light-sensitive metal-organic framework photoelectrochemical gated transistor for enantioselective discrimination of monosaccharides
In this study, a light-sensitive organic photoelectrochemical transistor (OPECT) with homochiral character is developed for enantiomer discrimination. Demonstrated with the discrimination of glucose enantiomers, the photoelectrochemically active gate electrode is prepared by integrating Au nanoparticles (AuNPs) and a chiral Cu(II)-metal-organic framework (c-CuMOF) onto TiO2 nanotube arrays (TNT). The captured glucose enantiomers are oxidized to hydrogen peroxide (H2O2) by the oxidase-mimicking AuNPs-loaded c-CuMOF. Based on the confinement effect of the mesopocket structure of the c-CuMOF and the remarkable charge transfer...
Source: Biosensors and Bioelectronics - May 1, 2024 Category: Biotechnology Authors: Jian-Hong Zhu Haiquan Wang Junli Guo Junjian Zhao Zhida Gao Yan-Yan Song Chenxi Zhao Source Type: research
Softening implantable bioelectronics: Material designs, applications, and future directions
This article reviews recent research and development in softening materials and designs for implantable bioelectronics. Examples featuring tissue-penetrating and conformal softening devices highlight the promising potential of these approaches in biomedical applications. A concluding section delves into current challenges and outlines future directions for softening implantable device technologies, underscoring their pivotal role in propelling the evolution of next-generation bioelectronics.PMID:38692223 | DOI:10.1016/j.bios.2024.116328 (Source: Biosensors and Bioelectronics)
Source: Biosensors and Bioelectronics - May 1, 2024 Category: Biotechnology Authors: Subin Oh Simok Lee Sung Woo Kim Choong Yeon Kim Eun Young Jeong Juhyun Lee Do A Kwon Jae-Woong Jeong Source Type: research
Integrated molybdenum single atom array sensors with multichannels for nitrite detection in foods
In this study, an integrated array sensor for NO2- detection is developed based on molybdenum single atom material (IMSMo-SAC) using high-resolution electrohydrodynamic (EHD) printing technology. The sensor comprises three components: a printed electrode array, multichannels designed on polydimethylsiloxane (PDMS) and an electronic signal process device with bluetooth. By utilizing Mo-SAC to facilitate electron transfer during the redox reaction, rapid and efficient detection of NO2- can be achieved. The sensor has a wide linear range of 0.1 μM-107.8 mM, a low detection limit of 33 nM and a high sensitivity of 0.637 mA-1m...
Source: Biosensors and Bioelectronics - May 1, 2024 Category: Biotechnology Authors: Mei Ding Kangling Tian Jingwen Wang Yuhang Liu Guangxuan Hu Yan Zheng Shaohui Lei Jiayue Sun Hong Bin Yang Fang Xin Hu Source Type: research
Buckling forces and the wavy folds between pleural epithelial cells
Biosystems. 2024 Apr 29:105216. doi: 10.1016/j.biosystems.2024.105216. Online ahead of print.ABSTRACTCell shapes in tissues are affected by the biophysical interaction between cells. Tissue forces can influence specific cell features such as cell geometry and cell surface area. Here, we examined the 2-dimensional shape, size, and perimeter of pleural epithelial cells at various lung volumes. We demonstrated a 1.53-fold increase in 2-dimensional cell surface area and a 1.43-fold increase in cell perimeter at total lung capacity compared to residual lung volume. Consistent with previous results, close inspection of the pleur...
Source: Biosystems - May 1, 2024 Category: Biotechnology Authors: Joseph Sutlive Betty S Liu Stacey A Kwan Jennifer M Pan Kun Gou Rongguang Xu Ali B Ali Hassan A Khalil Maximilian Ackermann Zi Chen Steven J Mentzer Source Type: research
Sortase A-Based Post-translational Modifications on Encapsulin Nanocompartments
Biomacromolecules. 2024 Apr 30. doi: 10.1021/acs.biomac.3c01415. Online ahead of print.ABSTRACTProtein-based encapsulin nanocompartments, known for their well-defined structures and versatile functionalities, present promising opportunities in the fields of biotechnology and nanomedicine. In this investigation, we effectively developed a sortase A-mediated protein ligation system in Escherichia coli to site-specifically attach target proteins to encapsulin, both internally and on its surfaces without any further in vitro steps. We explored the potential applications of fusing sortase enzyme and a protease for post-translat...
Source: Biomacromolecules - May 1, 2024 Category: Biochemistry Authors: Seyed Hossein Helalat Rodrigo Coronel T éllez Ehsan Ansari Dezfouli Yi Sun Source Type: research
Synthetic reprogramming of plant developmental and biochemical pathways
Curr Opin Biotechnol. 2024 Apr 30;87:103139. doi: 10.1016/j.copbio.2024.103139. Online ahead of print.ABSTRACTPlant synthetic biology (Plant SynBio) is an emerging field with the potential to enhance agriculture, human health, and sustainability. Integrating genetic tools and engineering principles, Plant SynBio aims to manipulate cellular functions and construct novel biochemical pathways to develop plants with new phenotypic traits, enhanced yield, and be able to produce natural products and pharmaceuticals. This review compiles research efforts in reprogramming plant developmental and biochemical pathways. We highlight ...
Source: Current Opinion in Biotechnology - May 1, 2024 Category: Biotechnology Authors: Shammi Akter Oscar Castaneda-M éndez Jes ús Beltrán Source Type: research
