Cloning, expression analysis and localization of < em > DAZL < /em > gene implicated in germ cell development of male Hezuo pig
Anim Biotechnol. 2023 Sep 6:1-15. doi: 10.1080/10495398.2023.2249953. Online ahead of print.ABSTRACTDeleted in azoospermia-like (DAZL) is essential for mammalian testicular function and spermatogenesis. To explore the molecular characterization, expression patterns, and cellular localization of the DAZL in Hezuo pig testes, testicular tissue was isolated from Hezuo pig at five development stages including 30 days old (30 d), 90 days old (90 d), 120 days old (120 d), 180 days old (180 d), and 240 days old (240 d). DAZL cDNA was first cloned using the RT-PCR method, and its molecular characterization was analyzed using relev...
Source: Animal Biotechnology - September 6, 2023 Category: Biotechnology Authors: Yuran Tang Bo Zhang Haixia Shi Zunqiang Yan Pengfei Wang Qiaoli Yang Xiaoyu Huang Jiyou Li Zike Wang Shuangbao Gun Source Type: research
Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives
Mol Cell Biochem. 2023 Sep 2. doi: 10.1007/s11010-023-04840-x. Online ahead of print.ABSTRACTSpermatogenesis, a key part of the spermiation process, is regulated by a combination of key cells, such as primordial germ cells, spermatogonial stem cells, and somatic cells, such as Sertoli cells. Abnormal spermatogenesis can lead to azoospermia, testicular tumors, and other diseases related to male infertility. The application of single-cell RNA sequencing (scRNA-seq) technology in male reproduction is gradually increasing with its unique insight into deep mining and analysis. The data cover different periods of neonatal, prepu...
Source: Molecular and Cellular Biochemistry - September 2, 2023 Category: Biochemistry Authors: Hanbo Jia Wei Wang Zhaowen Zhou Zhiyi Chen Zijun Lan Hao Bo Liqing Fan Source Type: research
Evaluation of chemical castration using intra-testicular injection of zinc gluconate into the testis of the male donkey versus surgical castration: antimullerian hormone as an endpoint marker
CONCLUSION: Intra-testicular injection of zinc gluconate resulted in histopathological and ultrasonographic testicular changes in adult male donkeys, which may affect their reproductive potential. However, it did not significantly alter serum testosterone or AMH levels, indicating that it cannot be used as a substitute for surgical castration in male donkeys.PMID:37660034 | DOI:10.1186/s12917-023-03694-1 (Source: Cell Research)
Source: Cell Research - September 2, 2023 Category: Cytology Authors: Mohamed A Hamed Yahia A Amin Ragab Hassan Mohamed Mohamed El-Adl Shefaa M Bazeed Ahmed Abdou Elnegiry Hossam Hassan Shawki Al-Lethie A Al-Lethie Source Type: research
Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives
Mol Cell Biochem. 2023 Sep 2. doi: 10.1007/s11010-023-04840-x. Online ahead of print.ABSTRACTSpermatogenesis, a key part of the spermiation process, is regulated by a combination of key cells, such as primordial germ cells, spermatogonial stem cells, and somatic cells, such as Sertoli cells. Abnormal spermatogenesis can lead to azoospermia, testicular tumors, and other diseases related to male infertility. The application of single-cell RNA sequencing (scRNA-seq) technology in male reproduction is gradually increasing with its unique insight into deep mining and analysis. The data cover different periods of neonatal, prepu...
Source: Molecular and Cellular Biochemistry - September 2, 2023 Category: Biochemistry Authors: Hanbo Jia Wei Wang Zhaowen Zhou Zhiyi Chen Zijun Lan Hao Bo Liqing Fan Source Type: research
Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives
Mol Cell Biochem. 2023 Sep 2. doi: 10.1007/s11010-023-04840-x. Online ahead of print.ABSTRACTSpermatogenesis, a key part of the spermiation process, is regulated by a combination of key cells, such as primordial germ cells, spermatogonial stem cells, and somatic cells, such as Sertoli cells. Abnormal spermatogenesis can lead to azoospermia, testicular tumors, and other diseases related to male infertility. The application of single-cell RNA sequencing (scRNA-seq) technology in male reproduction is gradually increasing with its unique insight into deep mining and analysis. The data cover different periods of neonatal, prepu...
Source: Molecular and Cellular Biochemistry - September 2, 2023 Category: Biochemistry Authors: Hanbo Jia Wei Wang Zhaowen Zhou Zhiyi Chen Zijun Lan Hao Bo Liqing Fan Source Type: research
Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives
Mol Cell Biochem. 2023 Sep 2. doi: 10.1007/s11010-023-04840-x. Online ahead of print.ABSTRACTSpermatogenesis, a key part of the spermiation process, is regulated by a combination of key cells, such as primordial germ cells, spermatogonial stem cells, and somatic cells, such as Sertoli cells. Abnormal spermatogenesis can lead to azoospermia, testicular tumors, and other diseases related to male infertility. The application of single-cell RNA sequencing (scRNA-seq) technology in male reproduction is gradually increasing with its unique insight into deep mining and analysis. The data cover different periods of neonatal, prepu...
Source: Molecular and Cellular Biochemistry - September 2, 2023 Category: Biochemistry Authors: Hanbo Jia Wei Wang Zhaowen Zhou Zhiyi Chen Zijun Lan Hao Bo Liqing Fan Source Type: research
Single-cell RNA sequencing technology in human spermatogenesis: Progresses and perspectives
Mol Cell Biochem. 2023 Sep 2. doi: 10.1007/s11010-023-04840-x. Online ahead of print.ABSTRACTSpermatogenesis, a key part of the spermiation process, is regulated by a combination of key cells, such as primordial germ cells, spermatogonial stem cells, and somatic cells, such as Sertoli cells. Abnormal spermatogenesis can lead to azoospermia, testicular tumors, and other diseases related to male infertility. The application of single-cell RNA sequencing (scRNA-seq) technology in male reproduction is gradually increasing with its unique insight into deep mining and analysis. The data cover different periods of neonatal, prepu...
Source: Molecular and Cellular Biochemistry - September 2, 2023 Category: Biochemistry Authors: Hanbo Jia Wei Wang Zhaowen Zhou Zhiyi Chen Zijun Lan Hao Bo Liqing Fan Source Type: research
Evaluation of chemical castration using intra-testicular injection of zinc gluconate into the testis of the male donkey versus surgical castration: antimullerian hormone as an endpoint marker
Chemical castration of male animals is an alternative to surgical castration for inducing azoospermia, consequent sterility. Intra-testicular injection of zinc gluconate has been used for chemical castration i... (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - September 2, 2023 Category: Veterinary Research Authors: Mohamed A. Hamed, Yahia A. Amin, Ragab Hassan Mohamed, Mohamed El-Adl, Shefaa M. Bazeed, Ahmed Abdou Elnegiry, Hossam Hassan Shawki and Al-Lethie A. Al-lethie Tags: Research Source Type: research
Effects of insulin-like growth factor-1 on the proliferation and apoptosis of stallion testicular cells under normal and heat stress culture conditions
This study investigated the effect of heat stress on stallion testicular cells (TCs) and the effect of insulin-like growth factor (IGF)-1 on TC viability, proliferation, and apoptosis, including different stages of germ cells. TCs were divided into control or treatment groups with 0.01, 0.1, 1, 10, and 100 ng/mL of recombinant human IGF-1 (rhIGF-1) for 24 h at 34 °C and 37 °C. The population and viability were measured before and after treatment. The effects of rhIGF-1 on TC viability, proliferation, and apoptosis were determined using RT-qPCR. Proliferating cell nuclear antigen (PCNA) and marker of proliferation Ki-67 (...
Source: Animal Reproduction Science - August 26, 2023 Category: Zoology Authors: Muhammad Shakeel Minjung Yoon Source Type: research
Effects of insulin-like growth factor-1 on the proliferation and apoptosis of stallion testicular cells under normal and heat stress culture conditions
This study investigated the effect of heat stress on stallion testicular cells (TCs) and the effect of insulin-like growth factor (IGF)-1 on TC viability, proliferation, and apoptosis, including different stages of germ cells. TCs were divided into control or treatment groups with 0.01, 0.1, 1, 10, and 100 ng/mL of recombinant human IGF-1 (rhIGF-1) for 24 h at 34 °C and 37 °C. The population and viability were measured before and after treatment. The effects of rhIGF-1 on TC viability, proliferation, and apoptosis were determined using RT-qPCR. Proliferating cell nuclear antigen (PCNA) and marker of proliferation Ki-67 (...
Source: Animal Reproduction Science - August 26, 2023 Category: Zoology Authors: Muhammad Shakeel Minjung Yoon Source Type: research
Effects of insulin-like growth factor-1 on the proliferation and apoptosis of stallion testicular cells under normal and heat stress culture conditions
This study investigated the effect of heat stress on stallion testicular cells (TCs) and the effect of insulin-like growth factor (IGF)-1 on TC viability, proliferation, and apoptosis, including different stages of germ cells. TCs were divided into control or treatment groups with 0.01, 0.1, 1, 10, and 100 ng/mL of recombinant human IGF-1 (rhIGF-1) for 24 h at 34 °C and 37 °C. The population and viability were measured before and after treatment. The effects of rhIGF-1 on TC viability, proliferation, and apoptosis were determined using RT-qPCR. Proliferating cell nuclear antigen (PCNA) and marker of proliferation Ki-67 (...
Source: Animal Reproduction Science - August 26, 2023 Category: Zoology Authors: Muhammad Shakeel Minjung Yoon Source Type: research
Effects of insulin-like growth factor-1 on the proliferation and apoptosis of stallion testicular cells under normal and heat stress culture conditions
This study investigated the effect of heat stress on stallion testicular cells (TCs) and the effect of insulin-like growth factor (IGF)-1 on TC viability, proliferation, and apoptosis, including different stages of germ cells. TCs were divided into control or treatment groups with 0.01, 0.1, 1, 10, and 100 ng/mL of recombinant human IGF-1 (rhIGF-1) for 24 h at 34 °C and 37 °C. The population and viability were measured before and after treatment. The effects of rhIGF-1 on TC viability, proliferation, and apoptosis were determined using RT-qPCR. Proliferating cell nuclear antigen (PCNA) and marker of proliferation Ki-67 (...
Source: Animal Reproduction Science - August 26, 2023 Category: Zoology Authors: Muhammad Shakeel Minjung Yoon Source Type: research
Genes, Vol. 14, Pages 1685: Mammal Reproductive Homeobox (Rhox) Genes: An Update of Their Involvement in Reproduction and Development
Vialard
The reproductive homeobox on the X chromosome (RHOX) genes were first identified in the mouse during the 1990s and have a crucial role in reproduction. In various transcription factors with a key regulatory role, the homeobox sequence encodes a &ldquo;homeodomain&rdquo; DNA-binding motif. In the mouse, there are three clusters of Rhox genes (&alpha;, &beta;, and &gamma;) on the X chromosome. Each cluster shows temporal and/or quantitative collinearity, which regulates the progression of the embryonic development process. Although the RHOX family is conserved in mammals, t...
Source: Genes - August 25, 2023 Category: Genetics & Stem Cells Authors: Morgane Le Beulze C écile Daubech Aissatu Balde-Camara Farah Ghieh Fran çois Vialard Tags: Review Source Type: research
Altered G-Protein Transduction Protein Gene Expression in the Testis of Infertile Patients with Nonobstructive Azoospermia
DNA and Cell Biology, Ahead of Print. (Source: DNA and Cell Biology)
Source: DNA and Cell Biology - August 22, 2023 Category: Cytology Authors: Danial Hashemi Karoii Hossein Azizi Thomas Skutella Source Type: research
Scrutinizing the human TEX genes in the context of human male infertility
ConclusionOur findings concerning the X-linked genesTEX13B,TEX13C, andFAM9A (TEX39A) contradict previous reports and will decrease false-positive reports in genetic diagnostics of azoospermic men. (Source: Andrology)
Source: Andrology - August 19, 2023 Category: Urology & Nephrology Authors: Marie. H. Sieper,
Avinash S. Gaikwad,
Marion Fros,
Philipp Weber,
Sara Di Persio,
Manon S. Oud,
Sabine Kliesch,
Nina Neuhaus,
Birgit Stallmeyer,
Frank T üttelmann,
Margot J. Wyrwoll Tags: ORIGINAL ARTICLE Source Type: research
