Follicle-stimulating hormone and Inhibin B as predictors of successful sperm retrieval in men undergoing testicular sperm extraction: A review of 44 cases and meta-analysis
Afr J Reprod Health. 2023 Jun;27(6):51-59. doi: 10.29063/ajrh2023/v27i6.6.ABSTRACTThe aim of the study and meta-analysis was to evaluate the predictive value of follicle-stimulating hormone (FSH) and inhibin B in sperm retrieval in men with non-obstructive azoospermia NOA. A total of 44 patients with a mean age of 36.1 years (SD=+/- 6.17) was included. We had 19 patients with successful sperm retrieval and 25 with failure. All patients had a normal karyotype. There was no difference between groups regarding patients mean age, prolactin or FSH. Patients with successful sperm retrieval had a significantly higher inhibin B le...
Source: African Journal of Reproductive Health - September 16, 2023 Category: African Health Authors: Drago ş Puia Adelina Miron Daniel Radavoi Viorel Jinga C ătălin Pricop Source Type: research
Novel MEIOB pathogenic variants including a homozygous non-canonical splicing variant, cause meiotic arrest and human non-obstructive azoospermia
In this study, we identified novel compound heterozygous MEIOB variants (c.814C > T: p.R272X and c.976G > A: p.A326T) and a previously undescribed homozygous non-canonical splicing variant of MEIOB (c.528 + 3A > C) in two NOA-affected individuals from two irrelevant Chinese families. MEIOB missense variant (p.A326T) significantly reduced protein abundance and nonsense variant (p.R272X) produced a truncated protein. Both of two variants impaired the MEIOB-SPATA22 interaction. The MEIOB non-canonical splicing variant resulted in whole Exon 6 skipping by minigene assay, which was predicted to produce a frameshift tru...
Source: Clinical Genetics - September 16, 2023 Category: Genetics & Stem Cells Authors: Xiaoyu Zhu Kaiqin Hu Huiru Cheng Huan Wu Kuokuo Li Yang Gao Mingrong Lv Chuan Xu Hao Geng Qunshan Shen Yunxia Cao Xiaojin He Dongdong Tang Rui Guo Source Type: research
Follicle-stimulating hormone and Inhibin B as predictors of successful sperm retrieval in men undergoing testicular sperm extraction: A review of 44 cases and meta-analysis
Afr J Reprod Health. 2023 Jun;27(6):51-59. doi: 10.29063/ajrh2023/v27i6.6.ABSTRACTThe aim of the study and meta-analysis was to evaluate the predictive value of follicle-stimulating hormone (FSH) and inhibin B in sperm retrieval in men with non-obstructive azoospermia NOA. A total of 44 patients with a mean age of 36.1 years (SD=+/- 6.17) was included. We had 19 patients with successful sperm retrieval and 25 with failure. All patients had a normal karyotype. There was no difference between groups regarding patients mean age, prolactin or FSH. Patients with successful sperm retrieval had a significantly higher inhibin B le...
Source: African Journal of Reproductive Health - September 16, 2023 Category: African Health Authors: Drago ş Puia Adelina Miron Daniel Radavoi Viorel Jinga C ătălin Pricop Source Type: research
Sperm Production is Stable Over Time for Men with Azoospermia Factor C (AZFc) Y-Chromosome Microdeletions
(Source: Fertility and Sterility)
Source: Fertility and Sterility - September 15, 2023 Category: Reproduction Medicine Authors: Jessica A. Marinaro, Nahid Punjani, Jonathan Gal, Anna Mielnik, Peter N. Schlegel Tags: Research letter Source Type: research
Sperm production is stable over time for men with azoospermia factor c Y-chromosome microdeletions
It has been suggested that men with azoospermia factor c (AZFc) Y-chromosome microdeletions (YCMDs) experience a temporal decline in sperm production and should consider fertility preservation strategies in early adulthood (1). These claims were initially based on small, retrospective studies and case reports (2 –4), although a larger, more recent study similarly found that younger patients with deleted AZFc (age 20–30 years) had more ejaculated sperm than older patients (31–40 years); however, this study did not follow the same patients over time, making it difficult to assess temporal changes for in dividual men (5...
Source: Fertility and Sterility - September 15, 2023 Category: Reproduction Medicine Authors: Jessica A. Marinaro, Nahid Punjani, Jonathan Gal, Anna Mielnik, Peter N. Schlegel Tags: Research letter Source Type: research
Tip ‐microVapour Fast Freezing: A novel easy method for cryopreserving severe oligozoospermic samples
Discussion and conclusionTip-microVapour Fast Freezing appears to be a very promising method to cryopreserve semen samples from severe oligozoospermic patients. (Source: Andrology)
Source: Andrology - September 14, 2023 Category: Urology & Nephrology Authors: Sara Dabizzi,
Costanza Calamai,
Selene Degl'Innocenti,
Luca Boni,
Linda Vignozzi,
Mario Maggi,
Elisabetta Baldi,
Monica Muratori Tags: ORIGINAL ARTICLE Source Type: research
A rare case of mosaic Klinefelter syndrome in a 45-year-old man leading to successful live birth through ejaculated spermatozoa: a case report and literature review
Conclusion: Although this case is exceedingly rare, it underscores the critical importance of exhausting all possibilities to facilitate biological parenthood in men with KS before considering alternative options such as sperm donation or adoption. By recognizing the potential for successful conception using ejaculated sperm in this population, we can provide individuals with mosaic KS the opportunity to fulfill their desire for biological offspring. (Source: Frontiers in Genetics)
Source: Frontiers in Genetics - September 13, 2023 Category: Genetics & Stem Cells Source Type: research
Copy number variations (CNVs) and karyotyping analysis in males with azoospermia and oligospermia
Considering the essential roles that genetic factors play in azoospermia and oligospermia, this study aims to identify abnormal chromosomes using karyotyping and CNVs and elucidate the associated genes in pati... (Source: BMC Medical Genomics)
Source: BMC Medical Genomics - September 8, 2023 Category: Genetics & Stem Cells Authors: Xing Xin, Peng Xu, Nan Wang, Yi Jiang, Jiaqiao Zhang, Shufang Li, Ying Zhu, Cong Zhang, Long Zhang, Hailong Huang, Ling Feng and Shaoshuai Wang Tags: Research Source Type: research
EAA/EMQN best practice guidelines for molecular diagnosis of Y ‐chromosomal microdeletions: State of the art 2023
AbstractTesting for AZoospermia Factor (AZF) deletions of the Y chromosome is a key component of the diagnostic workup of azoospermic and severely oligozoospermic men. This revision of the 2013 European Academy of Andrology (EAA) and EMQN CIC (previously known as the European Molecular Genetics Quality Network) laboratory guidelines summarizes recent clinically relevant advances and provides an update on the results of the external quality assessment program jointly offered by both organizations. A basic multiplex PCR reaction followed by a deletion extension analysis remains the gold-standard methodology to detect and cor...
Source: Andrology - September 7, 2023 Category: Urology & Nephrology Authors: Csilla Krausz,
Paulo Navarro ‐Costa,
Martina Wilke,
Frank Tüttelmann Tags: REVIEW ARTICLE Source Type: research
Quality of testicular spermatozoa improves with changes in composition of culture medium
ConclusionThe results demonstrated that in vitro incubation of testicular spermatozoa improved their motility more effectively than Ham ’s F10 medium in the short term (2 h), but had no effect on epididymal spermatozoa. Since the physiology of testicular spermatozoa is different from that of ejaculated spermatozoa, it seems that a special environment should be designed and used for each of them. (Source: Basic and Clinical Andrology)
Source: Basic and Clinical Andrology - September 7, 2023 Category: Urology & Nephrology Source Type: research
Cloning, expression analysis and localization of < em > DAZL < /em > gene implicated in germ cell development of male Hezuo pig
Anim Biotechnol. 2023 Sep 6:1-15. doi: 10.1080/10495398.2023.2249953. Online ahead of print.ABSTRACTDeleted in azoospermia-like (DAZL) is essential for mammalian testicular function and spermatogenesis. To explore the molecular characterization, expression patterns, and cellular localization of the DAZL in Hezuo pig testes, testicular tissue was isolated from Hezuo pig at five development stages including 30 days old (30 d), 90 days old (90 d), 120 days old (120 d), 180 days old (180 d), and 240 days old (240 d). DAZL cDNA was first cloned using the RT-PCR method, and its molecular characterization was analyzed using relev...
Source: Animal Biotechnology - September 6, 2023 Category: Biotechnology Authors: Yuran Tang Bo Zhang Haixia Shi Zunqiang Yan Pengfei Wang Qiaoli Yang Xiaoyu Huang Jiyou Li Zike Wang Shuangbao Gun Source Type: research
Cloning, expression analysis and localization of < em > DAZL < /em > gene implicated in germ cell development of male Hezuo pig
Anim Biotechnol. 2023 Sep 6:1-15. doi: 10.1080/10495398.2023.2249953. Online ahead of print.ABSTRACTDeleted in azoospermia-like (DAZL) is essential for mammalian testicular function and spermatogenesis. To explore the molecular characterization, expression patterns, and cellular localization of the DAZL in Hezuo pig testes, testicular tissue was isolated from Hezuo pig at five development stages including 30 days old (30 d), 90 days old (90 d), 120 days old (120 d), 180 days old (180 d), and 240 days old (240 d). DAZL cDNA was first cloned using the RT-PCR method, and its molecular characterization was analyzed using relev...
Source: Animal Biotechnology - September 6, 2023 Category: Biotechnology Authors: Yuran Tang Bo Zhang Haixia Shi Zunqiang Yan Pengfei Wang Qiaoli Yang Xiaoyu Huang Jiyou Li Zike Wang Shuangbao Gun Source Type: research
Cloning, expression analysis and localization of < em > DAZL < /em > gene implicated in germ cell development of male Hezuo pig
Anim Biotechnol. 2023 Sep 6:1-15. doi: 10.1080/10495398.2023.2249953. Online ahead of print.ABSTRACTDeleted in azoospermia-like (DAZL) is essential for mammalian testicular function and spermatogenesis. To explore the molecular characterization, expression patterns, and cellular localization of the DAZL in Hezuo pig testes, testicular tissue was isolated from Hezuo pig at five development stages including 30 days old (30 d), 90 days old (90 d), 120 days old (120 d), 180 days old (180 d), and 240 days old (240 d). DAZL cDNA was first cloned using the RT-PCR method, and its molecular characterization was analyzed using relev...
Source: Animal Biotechnology - September 6, 2023 Category: Biotechnology Authors: Yuran Tang Bo Zhang Haixia Shi Zunqiang Yan Pengfei Wang Qiaoli Yang Xiaoyu Huang Jiyou Li Zike Wang Shuangbao Gun Source Type: research
