GSE237388 Single-cell RNA sequencing of CD11b+ Ly6C+ Ly6G- sorted bone marrow cells from radiation control and severe aplastic anemia mice
Contributors : Grazda Rachel ; MacNamara C KatherineSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDuring immune-mediated severe aplastic anemia (SAA) monocytes (CD11b+ Ly6C+ Ly6G-) signficantly increase by both frequency and number within the bone marrow. We isolated BM monocytes from F1 hybrid mice (C57BL/6;Balb/c) induced with SAA via the splenocyte-transfer model. We utilized single-cell RNA sequencing (scRNA-seq) to analyze the heterogenity of BM monocytes during SAA. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - July 19, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
A well curated cost-effective next generation sequencing panel identifies a diverse landscape of pathogenic and novel germline variants in bone marrow failure cohort in a resource constraint setting
Current study is a 4 year experience in diagnosis and screening of inherited and immune bone marrow failure cases using a targeted sequencing panel. A total of 171 cases underwent targeted NGS and were categorized as suspected inherited bone marrow failure syndrome (IBMFS) group (106; 62%) and immune/idiopathic aplastic anemia (IAA) group (65; 38%) based on clinical and laboratory criteria. 110 (64%) were pediatric (0-12 years) and 61 (36%) adolescent and adults (13-47 years). In suspected IBMFS group, 47 (44%) and in IAA group, 8 (12%) revealed a likely germline pathogenic variation. (Source: Journal of Molecular Diagnostics)
Source: Journal of Molecular Diagnostics - July 17, 2023 Category: Pathology Authors: Swetha Pallavelangini, Gnanamani Senguttuvan, Prateek Bhatia, Prashant Chhabra, Minu Singh, Alka Khadwal, Arihant Jain, Pankaj Sharma, Rozy Thakur, Sreejesh Sreedharanunni, Deepak Bansal, Richa Jain, Srinivasan Peyam, Sonali Mohapatra, Ankur Jindal, Deept Tags: Regular Article Source Type: research
A Well-Curated Cost-Effective Next-Generation Sequencing Panel Identifies a Diverse Landscape of Pathogenic and Novel Germline Variants in a Bone Marrow Failure Cohort in a Resource-Constraint Setting
The current study is a 4-year experience in diagnosis and screening of inherited and immune bone marrow failure cases using a targeted sequencing panel. A total of 171 cases underwent targeted next-generation sequencing and were categorized as suspected inherited bone marrow failure syndrome (IBMFS) group (106; 62%) and immune/idiopathic aplastic anemia (IAA) group (65; 38%) based on clinical and laboratory criteria. A total of 110 (64%) were pediatric (aged 0 to 12 years) patients and 61 (36%) were adolescent and adult (aged 13 to 47 years) patients. (Source: Journal of Molecular Diagnostics)
Source: Journal of Molecular Diagnostics - July 17, 2023 Category: Pathology Authors: Swetha Pallavelangini, Gnanamani Senguttuvan, Prateek Bhatia, Prashant Chhabra, Minu Singh, Alka Khadwal, Arihant Jain, Pankaj Sharma, Rozy Thakur, Sreejesh Sreedharanunni, Deepak Bansal, Richa Jain, Srinivasan Peyam, Sonali Mohapatra, Ankur Jindal, Deept Tags: Regular article Source Type: research
Effects of Ruxolitinib on Murine Regulatory T Cells Are Immune Context Dependent
Aplastic anemia (AA) is a bone marrow failure (BMF) disorder mediated by immune dysfunction resulting in pancytopenia and marrow hypoplasia. To study immune AA experimentally, BMF can be induced in mice with major histocompatibility complex-mismatched lymph node (LN) cell infusion after sublethal total body irradiation (TBI). Based on the suppressive effect of the JAK 1/2 inhibitor ruxolitinib (RUX) on T cell activation and inflammatory cytokine production, we previously studied RUX in our murine BMF model. (Source: Experimental Hematology)
Source: Experimental Hematology - July 16, 2023 Category: Hematology Authors: Nidhi Aggarwal, Ash Lee Manley, Jichun Chen, Emma M. Groarke, Xingmin Feng, Neal S. Young Tags: Brief Communication Source Type: research
Effects of ruxolitinib on murine regulatory T cells are immune-context dependent
Aplastic anemia (AA) is a bone marrow failure (BMF) disorder mediated by immune dysfunction resulting in pancytopenia and marrow hypoplasia. To study immune AA experimentally, BMF can be induced in mice with major histocompatibility complex-mismatched lymph node (LN) cell infusion after sublethal total body irradiation (TBI). Based on the suppressive effect of the JAK 1/2 inhibitor ruxolitinib (RUX) on T-cell activation and inflammatory cytokine production, we previously studied RUX in our murine BMF model. (Source: Experimental Hematology)
Source: Experimental Hematology - July 16, 2023 Category: Hematology Authors: Nidhi Aggarwal, Ash Lee Manley, Jichun Chen, Emma M. Groarke, Xingmin Feng, Neal S. Young Tags: Brief Communication Source Type: research
A clinical analysis of Candida tropicalis bloodstream infections associated with hematological diseases, and antifungal susceptibility: a retrospective survey
ConclusionHematological disease patients with C. tropicalis bloodstream infections had a high mortality rate, and early antifungal therapy significantly reduced mortality. Candida tropicalis was highly resistant to azole drugs and sensitive to flucytosine and amphotericin B. According to our study, the preferred agent is amphotericin B and drug combinations should be considered for severe infections. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - July 14, 2023 Category: Microbiology Source Type: research
Haploidentical haematopoietic stem cell transplant in paediatric patients with acquired hypocellular bone marrow failures
Acquired hypocellular bone marrow failures without underlying genetic cause (AHBMF) during childhood are usually diagnosed either as Severe Acquired Aplastic Anaemia (SAA) or Refractory Cytopenia of Childhood (RCC). SAA and RCC are considered separate entities, but it can be challenging to differentiate them. Although SAA and RCC can be treated with relative success with immunosuppressive therapy (IST) with ATG and cyclosporine, a Matched donor haematopoietic stem cell transplantation (HSCT) is the treatment of choice1,2,3. (Source: Biology of Blood and Marrow Transplantation)
Source: Biology of Blood and Marrow Transplantation - July 14, 2023 Category: Hematology Authors: Victor Quintero, David Bueno-S ánchez, Yasmina Mozo-Del Castillo, Andrea Urtasun-Erburu, Luisa Sisinni, Mónica López-Duarte, José María Pérez-Hurtado, José Luis Fuster, Marta González-Vicent, Antonio Pérez-Martínez, Cristina Diaz-de-Heredia Source Type: research
Haploidentical Hematopoietic Stem Cell Transplantation in Pediatric Patients with Acquired Hypocellular Bone Marrow Failure
Acquired hypocellular bone marrow failure without underlying genetic cause (AHBMF) during childhood is usually diagnosed as either severe acquired aplastic anemia (SAA) or refractory cytopenia of childhood (RCC). SAA and RCC are considered separate entities, but differentiating them can be challenging. Although SAA and RCC can be treated with relative success with immunosuppressive therapy (IST) with antithymocyte globulin and cyclosporine, matched donor hematopoietic stem cell transplantation (HSCT) is the treatment of choice [1 –3]. (Source: Biology of Blood and Marrow Transplantation)
Source: Biology of Blood and Marrow Transplantation - July 14, 2023 Category: Hematology Authors: Victor Quintero, David Bueno-S ánchez, Yasmina Mozo-Del Castillo, Andrea Urtasun-Erburu, Luisa Sisinni, Mónica López-Duarte, José María Pérez-Hurtado, José Luis Fuster, Marta González-Vicent, Antonio Pérez-Martínez, Cristina Diaz-de-Heredia, Gru Tags: Pediatric Source Type: research
Comparison of haploidentical –allogeneic hematopoietic stem cell transplantation and intensive immunosuppressive therapy for patients with severe aplastic anemia with an absolute neutrophil count of zero: a retrospective study
AbstractA retrospective analysis was conducted based on the clinical data from 60 patients older than 16 years from January 2016 to January 2021. All the patients were newly diagnosed with severe aplastic anemia (SAA) with an absolute neutrophil count (ANC) of zero. We compared the hematological response and survival of haploidentical –allogeneic hematopoietic stem cell transplantation (HID-HSCT) (n = 25) and intensive immunosuppressive therapy (IST) (n = 35) treatments. At six months, the overall response rate and complete response were significantly higher in the HID-HSCT group than those in the IST group (84.0% vs. 40...
Source: Annals of Hematology - July 13, 2023 Category: Hematology Source Type: research
Long term survivors in severe aplastic anemia: standard mortality rates now comparable to controls?
Haematologica. 2023 Jul 13. doi: 10.3324/haematol.2023.283523. Online ahead of print.ABSTRACTNot available.PMID:37439355 | DOI:10.3324/haematol.2023.283523 (Source: Haematologica)
Source: Haematologica - July 13, 2023 Category: Hematology Authors: Andrea Bacigalupo Source Type: research
Salidroside affects the Th17/Treg cell balance in aplastic anemia via the STAT3/HIF-1 α/RORγt pathway
CONCLUSION: Salidroside has potential as a novel AA treatment by correcting the Th17/Treg imbalance through the STAT3/HIF-1α/RORγt pathway.PMID:37439434 | DOI:10.1080/13510002.2023.2225868 (Source: Redox Report : communications in free radical research)
Source: Redox Report : communications in free radical research - July 13, 2023 Category: Biochemistry Authors: Zenghui Liu Lixuan Chen Defang Xiong Yu Zhan Jiduo Liu Ling Ouyang Wancheng Chen Ziyuan Lu Tianqi Gao Xiaozhen Li Yanqun Zhou Xuekui Gu Yang Xiao Source Type: research
Long term survivors in severe aplastic anemia: standard mortality rates now comparable to controls?
Haematologica. 2023 Jul 13. doi: 10.3324/haematol.2023.283523. Online ahead of print.ABSTRACTNot available.PMID:37439355 | DOI:10.3324/haematol.2023.283523 (Source: Haematologica)
Source: Haematologica - July 13, 2023 Category: Hematology Authors: Andrea Bacigalupo Source Type: research
Salidroside affects the Th17/Treg cell balance in aplastic anemia via the STAT3/HIF-1 α/RORγt pathway
CONCLUSION: Salidroside has potential as a novel AA treatment by correcting the Th17/Treg imbalance through the STAT3/HIF-1α/RORγt pathway.PMID:37439434 | PMC:PMC10348039 | DOI:10.1080/13510002.2023.2225868 (Source: Redox Report : communications in free radical research)
Source: Redox Report : communications in free radical research - July 13, 2023 Category: Biochemistry Authors: Zenghui Liu Lixuan Chen Defang Xiong Yu Zhan Jiduo Liu Ling Ouyang Wancheng Chen Ziyuan Lu Tianqi Gao Xiaozhen Li Yanqun Zhou Xuekui Gu Yang Xiao Source Type: research
Long term survivors in severe aplastic anemia: standard mortality rates now comparable to controls?
Haematologica. 2023 Jul 13. doi: 10.3324/haematol.2023.283523. Online ahead of print.ABSTRACTNot available.PMID:37439355 | DOI:10.3324/haematol.2023.283523 (Source: Haematologica)
Source: Haematologica - July 13, 2023 Category: Hematology Authors: Andrea Bacigalupo Source Type: research
Salidroside affects the Th17/Treg cell balance in aplastic anemia via the STAT3/HIF-1 α/RORγt pathway
CONCLUSION: Salidroside has potential as a novel AA treatment by correcting the Th17/Treg imbalance through the STAT3/HIF-1α/RORγt pathway.PMID:37439434 | PMC:PMC10348039 | DOI:10.1080/13510002.2023.2225868 (Source: Redox Report : communications in free radical research)
Source: Redox Report : communications in free radical research - July 13, 2023 Category: Biochemistry Authors: Zenghui Liu Lixuan Chen Defang Xiong Yu Zhan Jiduo Liu Ling Ouyang Wancheng Chen Ziyuan Lu Tianqi Gao Xiaozhen Li Yanqun Zhou Xuekui Gu Yang Xiao Source Type: research
