Successful Treatment of Therapy-refractory Acanthamoeba Keratitis with Systemic Miltefosine and Topical Voriconazole
Klin Monbl Augenheilkd. 2024 Apr;241(4):406-408. doi: 10.1055/a-2196-6011. Epub 2024 Apr 23.NO ABSTRACTPMID:38653269 | DOI:10.1055/a-2196-6011 (Source: Klinische Monatsblatter fur Augenheilkunde)
Source: Klinische Monatsblatter fur Augenheilkunde - April 23, 2024 Category: Opthalmology Authors: Petra Schwarzer Frank Blaser Miriam Sellner Stephan Christian Rauthe Tamer Tandogan Christoph Tappeiner David Goldblum Source Type: research
Anti-Acanthamoeba metallopharmaceuticals: Amoebicidal activity and synergistic effect of copper(II) Â coordination compound
This study evaluates the amoebicidal activity of copper(II) coordination compounds in combination with chlorhexidine and the cytotoxicity to topical ocular application. These copper(II) coordination compounds were screened against Acanthamoeba castellanii trophozoites (ATCC 50492). The cytotoxicity on rabbit corneal cell line (ATCC-CCL 60) was performed. The compounds showed high amoebicidal potential, with inhibition of trophozoite viability above 80%. The Cp12 and Cp13 compounds showed Minimal Inhibitory Amoebicidal Concentration (MIAC) at 200 µM and mean inhibitory concentration (IC50) values lower than 10 µM. Against...
Source: Biometals - April 22, 2024 Category: Biochemistry Authors: Jaqueline da Silveira Ana Paula Cardoso Christiane Fernandes Adolfo Horn Junior Gabriella da Rosa Monte Machado Karin Silva Caumo Source Type: research
Anti-Acanthamoeba metallopharmaceuticals: Amoebicidal activity and synergistic effect of copper(II) Â coordination compound
This study evaluates the amoebicidal activity of copper(II) coordination compounds in combination with chlorhexidine and the cytotoxicity to topical ocular application. These copper(II) coordination compounds were screened against Acanthamoeba castellanii trophozoites (ATCC 50492). The cytotoxicity on rabbit corneal cell line (ATCC-CCL 60) was performed. The compounds showed high amoebicidal potential, with inhibition of trophozoite viability above 80%. The Cp12 and Cp13 compounds showed Minimal Inhibitory Amoebicidal Concentration (MIAC) at 200 µM and mean inhibitory concentration (IC50) values lower than 10 µM. Against...
Source: Biometals - April 22, 2024 Category: Biochemistry Authors: Jaqueline da Silveira Ana Paula Cardoso Christiane Fernandes Adolfo Horn Junior Gabriella da Rosa Monte Machado Karin Silva Caumo Source Type: research
Differentiation of acanthamoeba keratitis from other non-acanthamoeba keratitis: Risk factors and clinical features
by Shaker Osaywid Alreshidi, Jos é Manuel Vargas, Khabir Ahmad, Ahmed Yousef Alothman, Eman D. Albalawi, Abdulmohsen Almulhim, Saad Hamdan Alenezi, Hani Basher ALBalawi, Naif Mamdouh Alali, Faris Hashem, Mohanna Aljindan
IntroductionInfectious Keratitis is one of the most common ocular emergencies seen by ophthalmologists. Our aim is to identify the risk factors and clinical features of Acanthamoeba Keratitis (AK). MethodsThis retrospective chart review study was conducted at King Khaled Eye Specialist Hospital, Riyadh, Saudi Arabia, and included all the microbial keratitis cases, male and female patients of all ages. The...
Source: PLoS One - March 12, 2024 Category: Biomedical Science Authors: Shaker Osaywid Alreshidi Source Type: research
Comparison of therapeutic effects between big-bubble deep anterior lamellar keratoplasty and penetrating keratoplasty for medically unresponsive Acanthamoeba keratitis
To compare the outcomes of big-bubble deep anterior lamellar keratoplasty (BB-DALK) and penetrating keratoplasty (PKP) in the management of medically unresponsive Acanthamoeba keratitis (AK). (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - March 4, 2024 Category: Infectious Diseases Authors: Xiaolin Qi, Huilin Mao, Jinhui Liu, Yanling Dong, Man Du, Ting Liu, Ting Zhang, Xiuhai Lu and Hua Gao Tags: Research Source Type: research
Self-assembled micelles loaded with itraconazole as anti-Acanthamoeba nano-formulation
Arch Microbiol. 2024 Mar 4;206(4):134. doi: 10.1007/s00203-024-03854-3.ABSTRACTAcanthamoeba castellanii are opportunistic pathogens known to cause infection of the central nervous system termed: granulomatous amoebic encephalitis, that mostly effects immunocompromised individuals, and a sight threatening keratitis, known as Acanthamoeba keratitis, which mostly affects contact lens wearers. The current treatment available is problematic, and is toxic. Herein, an amphiphilic star polymer with AB2 miktoarms [A = hydrophobic poly(ℇ-Caprolacton) and B = hydrophilic poly (ethylene glycol)] was synthesized by ring opening polym...
Source: Archives of Microbiology - March 3, 2024 Category: Microbiology Authors: Komal Rao Muhammad Abdullah Usman Ahmed Hashi Isse Wehelie Muhammad Raza Shah Ruqaiyyah Siddiqui Naveed A Khan Bader S Alawfi Ayaz Anwar Source Type: research
Self-assembled micelles loaded with itraconazole as anti-Acanthamoeba nano-formulation
Arch Microbiol. 2024 Mar 4;206(4):134. doi: 10.1007/s00203-024-03854-3.ABSTRACTAcanthamoeba castellanii are opportunistic pathogens known to cause infection of the central nervous system termed: granulomatous amoebic encephalitis, that mostly effects immunocompromised individuals, and a sight threatening keratitis, known as Acanthamoeba keratitis, which mostly affects contact lens wearers. The current treatment available is problematic, and is toxic. Herein, an amphiphilic star polymer with AB2 miktoarms [A = hydrophobic poly(ℇ-Caprolacton) and B = hydrophilic poly (ethylene glycol)] was synthesized by ring opening polym...
Source: Archives of Microbiology - March 3, 2024 Category: Microbiology Authors: Komal Rao Muhammad Abdullah Usman Ahmed Hashi Isse Wehelie Muhammad Raza Shah Ruqaiyyah Siddiqui Naveed A Khan Bader S Alawfi Ayaz Anwar Source Type: research
Clinical outcomes and prognostic factors in Acanthamoeba keratitis
To report clinical findings and prognostic factors for visual and morphological outcomes in patients with Acanthamoeba keratitis (AK). (Source: Contact Lens and Anterior Eye)
Source: Contact Lens and Anterior Eye - January 13, 2024 Category: Opthalmology Authors: Rodrigo Vilares-Morgado, Ana Margarida Ferreira, Pedro Marques-Couto, Carolina Madeira, Ra úl Moreira, LuÃs Torrão, Pedro Neves-Cardoso, Ana Maria Cunha, Sandra Rebelo, João Pinheiro-Costa Source Type: research
Corneal ring infiltrate- far more than Acanthamoeba keratitis: review of pathophysiology, morphology, differential diagnosis and management
ConclusionCorneal ring infiltrate might be triggered not only by ocular infectious and non-infectious factors, but also by systemic conditions. Clinical assessment is crucial for empirical diagnosis. Furthermore, treatment is targeted towards the underlying condition but should begin with anti-infectious regimen until not proven otherwise. (Source: Journal of Ophthalmic Inflammation and Infection)
Source: Journal of Ophthalmic Inflammation and Infection - December 19, 2023 Category: Opthalmology Source Type: research
Incidence of Acanthamoeba Keratitis in the United Kingdom in 2015: A Prospective National Survey
CONCLUSIONS: This study provides the first published data on the nationwide incidence of AK in the United Kingdom. The findings confirm an increasing incidence of AK, particularly among contact lens wearers since 1997/8 to 1998/9. PRSCLs were identified as a significant risk factor compared with DDSCLs. Misdiagnosis and treatment delays remain an ongoing problem for patients with AK.PMID:38097200 | DOI:10.1097/ICO.0000000000003445 (Source: Herpes)
Source: Herpes - December 14, 2023 Category: Infectious Diseases Authors: Haneen Jasim Mariusz Grzeda Barny Foot Derek Tole Jeremy J Hoffman Source Type: research
SA59 Clinical Evidence on Acanthamoeba Keratitis Treatments: Systematic Literature Review
Acanthamoeba keratitis (AK) is a rare, severe, progressive corneal infection with no approved medicinal treatments. To understand the existing evidence-base on AK treatments, we conducted a systematic literature review (CRD42022345288). (Source: Value in Health)
Source: Value in Health - December 1, 2023 Category: International Medicine & Public Health Authors: L. Tofani, D. Bodicoat, A. Alves, A. Arteaga, V. Papa Source Type: research
CO144 A Trial-Based Health Utility Analysis in Patients with Acanthamoeba Keratitis
To estimate the health-related quality of life (HRQoL) of patients with Acanthamoeba keratitis (AK) and to explore the impact of Acanthamoeba infection, treatment and visual acuity on HRQoL. (Source: Value in Health)
Source: Value in Health - December 1, 2023 Category: International Medicine & Public Health Authors: A. Alves, E. Spaepen, M. De Francesco, F. Gissara, L. Tofani Source Type: research
A simple method for culturing Acanthamoeba from soft contact lens at a clinical laboratory of a hospital: Case report of Acanthamoeba keratitis
Culturing method forAcanthamoeba. (A) Translucent plaques (arrows) at the edge of bacterial white plaques as well as the inside after incubation for 4  days at 30°C on Sabouraud dextrose agar plate painted with heat-treated (at 60°C for 15 min)Escherichia coli. Saline washed with the contact lens was inoculated at the center of the dish. Parallel incubation at 23 °C resulted in less growth while incubation at 37°C resulted in no growth. (B and C), cysts (B) and trophozoite (C) by Giemsa stain (×1000) in the background of dead bacilli (E. coli). (D) Cysts (arrowheads) and trophozoite (arrow) dispersed in saline ( Ã...
Source: Clinical Case Reports - November 21, 2023 Category: General Medicine Authors: Toshihiko Matsuo,
Motoko Nose Tags: CASE REPORT Source Type: research
Acanthamoeba castellanii trophozoites escape killing by neutrophil extracellular traps using their 3'-nucleotidase/nuclease activity
Eur J Protistol. 2023 Oct 31;91:126032. doi: 10.1016/j.ejop.2023.126032. Online ahead of print.ABSTRACTAcanthamoeba castellanii is a free-living amoeba that acts as an opportunistic pathogen for humans and is the pathogenic agent of Acanthamoeba keratitis (AK). A. castellanii may present as proliferative and infective trophozoites or as resistant cysts during their life cycle. The immune response against AK is still poorly explored; however, it is well established that macrophages and neutrophils play essential roles in controlling corneal infection during the disease outcome. The release of NETs is one of the innate immun...
Source: European Journal of Protistology - November 10, 2023 Category: Microbiology Authors: Luiz Fernando Carvalho-Kelly Anita Leocadio Freitas-Mesquita Michelle T C Nascimento Claudia F Dick Eduarda de Souza-Maciel Natalia C Rochael Elvira M Saraiva Jos é Roberto Meyer-Fernandes Source Type: research
Identification and characterization of an ectophosphatase activity involved in Acanthamoeba castellanii adhesion to host cells
Eur J Protistol. 2023 Oct 13;91:126026. doi: 10.1016/j.ejop.2023.126026. Online ahead of print.ABSTRACTAcanthamoeba castellanii is a free-living amoeba and an opportunistic pathogen for humans that can cause encephalitis and, more commonly, Acanthamoeba keratitis. During its life cycle, A. castellanii may present as proliferative and infective trophozoites or resistant cysts. The adhesion of trophozoites to host cells is a key first step in the pathogenesis of infection. A major virulence protein of Acanthamoeba is a mannose-binding protein (MBP) that mediates the adhesion of amoebae to cell surfaces. Ectophosphatases are ...
Source: European Journal of Protistology - October 23, 2023 Category: Microbiology Authors: Luiz Fernando Carvalho-Kelly Anita Leocadio Freitas-Mesquita Clara Ferreira Pralon Eduarda de Souza-Maciel Jos é Roberto Meyer-Fernandes Source Type: research
