A simple method for culturing Acanthamoeba from soft contact lens at a clinical laboratory of a hospital: Case report of Acanthamoeba keratitis

Culturing method forAcanthamoeba. (A) Translucent plaques (arrows) at the edge of bacterial white plaques as well as the inside after incubation for 4  days at 30°C on Sabouraud dextrose agar plate painted with heat-treated (at 60°C for 15 min)Escherichia coli. Saline washed with the contact lens was inoculated at the center of the dish. Parallel incubation at 23 °C resulted in less growth while incubation at 37°C resulted in no growth. (B and C), cysts (B) and trophozoite (C) by Giemsa stain (×1000) in the background of dead bacilli (E. coli). (D) Cysts (arrowheads) and trophozoite (arrow) dispersed in saline ( ×400). (E) Many plaques of cysts in second isolation culture after incubation for 3 days at 30°C on Sabouraud dextrose agar plate painted with heat-treatedE. coli. Arrows indicate sites of inoculation of the initial plaques (E). Bar  = 20 μm in B, C, and D. Key Clinical MessageA simple culturing method forAcanthamoeba available at a clinical laboratory is a key for making timely diagnosis and starting treatment with topical 0.02% chlorhexidine gluconate eye drops, together with 0.1% miconazole or fluconazole eye drops.AbstractA 19-year-old woman with pain and injection in the right eye showed spotty corneal infiltration and radiating linear opacity. Suspicious ofAcanthamoeba keratitis, corneal scraping, and the soft contact lens were sent to in-house clinical laboratory to culture successfullyAcanthamoeba on Sabouraud dextrose agar plate painted wi...
Source: Clinical Case Reports - Category: General Medicine Authors: Tags: CASE REPORT Source Type: research