Biophysical insight into the binding mechanism of epigallocatechin-3-gallate and cholecalciferol to albumin and its preventive effect against AGEs formation: An in vitro and in silico approach
This article focusses on the interaction and antiglycating potential of (-)-Epigallocatechin-3-gallate (EGCG) and vitamin D in combination using various techniques. The formation of the HSA-EGCG and HSA-vitamin D complex was confirmed by UV and fluorescence spectroscopy. Thermodynamic analysis verified the spontaneity of reaction, and presence of hydrogen bonds and van der Waals interactions. FRET confirms high possibility of energy transfer. Cumulative antiglycation resulted in almost 60 % prevention in AGEs formation, decreased alterations at lysine and arginine, and reduced protein carbonylation. Secondary and tertiary ...
Source: International Journal of Biological Macromolecules - April 10, 2024 Category: Biochemistry Authors: Sana Siddiqui Rizwan Ahmad Yusra Ahmad Abul Faiz Faizy Shagufta Moin Source Type: research
Biophysical insight into the binding mechanism of epigallocatechin-3-gallate and cholecalciferol to albumin and its preventive effect against AGEs formation: An in vitro and in silico approach
This article focusses on the interaction and antiglycating potential of (-)-Epigallocatechin-3-gallate (EGCG) and vitamin D in combination using various techniques. The formation of the HSA-EGCG and HSA-vitamin D complex was confirmed by UV and fluorescence spectroscopy. Thermodynamic analysis verified the spontaneity of reaction, and presence of hydrogen bonds and van der Waals interactions. FRET confirms high possibility of energy transfer. Cumulative antiglycation resulted in almost 60 % prevention in AGEs formation, decreased alterations at lysine and arginine, and reduced protein carbonylation. Secondary and tertiary ...
Source: International Journal of Biological Macromolecules - April 10, 2024 Category: Biochemistry Authors: Sana Siddiqui Rizwan Ahmad Yusra Ahmad Abul Faiz Faizy Shagufta Moin Source Type: research
Coadministration of doxorubicin with vitamin D3, Lactobacillus acidophilus, and Lactobacillus casei in the 4T1 mouse model of breast cancer: anticancer and enteroprotective effects
Med Oncol. 2024 Apr 9;41(5):111. doi: 10.1007/s12032-024-02346-0.ABSTRACTThe use of doxorubicin (Dox) in the treatment of breast cancer negatively affects the intestines and other tissues. Many studies have proven that probiotics and vitamin D3 have antitumor and intestinal tissue-protecting properties. To achieve effectiveness and minimize side effects, the current study aims to administer Dox together with probiotics (Lactobacillus acidophilus and Lactobacillus casei) and vitamin D3. Forty-two female BALB/c inbred mice were divided into six groups: Group 1 (Control), Group 2 (Dox), Group 3 (Dox and probiotics), Group 4 (...
Source: Cancer Control - April 9, 2024 Category: Cancer & Oncology Authors: Mohammad Ali Mirzadeh Majid Eslami Ali Ghanbari Sam Zarbakhsh Sedighe Yosefi Abbas Pakdel Source Type: research
Coadministration of doxorubicin with vitamin D3, Lactobacillus acidophilus, and Lactobacillus casei in the 4T1 mouse model of breast cancer: anticancer and enteroprotective effects
AbstractThe use of doxorubicin (Dox) in the treatment of breast cancer negatively affects the intestines and other tissues. Many studies have proven that probiotics and vitamin D3 have antitumor and intestinal tissue-protecting properties. To achieve effectiveness and minimize side effects, the current study aims to administer Dox together with probiotics (Lactobacillus acidophilus andLactobacillus casei) and vitamin D3. Forty-two female BALB/c inbred mice were divided into six groups: Group 1 (Control), Group 2 (Dox), Group 3 (Dox and probiotics), Group 4 (Dox and vitamin D3), Group 5 (Dox, probiotics, and vitamin D3), an...
Source: Medical Oncology - April 9, 2024 Category: Cancer & Oncology Source Type: research
Effect of Antenatal Oral Vitamin D Supplementation on Serum 25(OH)D Concentration in Exclusively Breastfed Infants at 6 Months of age - A Randomized Double-Blind Placebo-Controlled Trial
CONCLUSIONS: Oral supplementation of vitamin D3 to pregnant women in the third trimester prevents severe hypovitaminosis D in the EBF infants at 6-months of age.PMID:38584410 (Source: Indian Pediatrics)
Source: Indian Pediatrics - April 8, 2024 Category: Pediatrics Authors: Prashant Prabhakar M M A Faridi Anju Aggarwal Rachna Aggarwal S V Madhu Rajeev Malhotra Source Type: research
Accumulated LPS induced by colitis altered the activities of vitamin D-metabolizing hydroxylases and decreased the generation of 25-hydroxyvitamin D
Chem Biol Interact. 2024 Apr 6:110997. doi: 10.1016/j.cbi.2024.110997. Online ahead of print.ABSTRACTIt is generally accepted that low vitamin D (VD) levels are associated with a high prevalence factor for Inflammatory bowel disease(IBD). IBD patients have observed higher levels of lipopolysaccharide(LPS), ALT, and AST than healthy people. Gut-derived LPS causes inflammatory injury in the liver and kidney. The VD-metabolizing mechanism is involved in the liver and kidney, which means IBD might impact VD metabolism. However, whether IBD affects VD metabolism has not been studied. In vitro LPS resulted in decreased CYP2R1 in...
Source: Chemico-Biological Interactions - April 8, 2024 Category: Molecular Biology Authors: Yingyu Lu Hao Chen Yonger Chen Liang Zhao Shaozhen Hou Source Type: research
Effect of Antenatal Oral Vitamin D Supplementation on Serum 25(OH)D Concentration in Exclusively Breastfed Infants at 6 Months of age - A Randomized Double-Blind Placebo-Controlled Trial
CONCLUSIONS: Oral supplementation of vitamin D3 to pregnant women in the third trimester prevents severe hypovitaminosis D in the EBF infants at 6-months of age.PMID:38584410 (Source: Indian Pediatrics)
Source: Indian Pediatrics - April 8, 2024 Category: Pediatrics Authors: Prashant Prabhakar M M A Faridi Anju Aggarwal Rachna Aggarwal S V Madhu Rajeev Malhotra Source Type: research
Accumulated LPS induced by colitis altered the activities of vitamin D-metabolizing hydroxylases and decreased the generation of 25-hydroxyvitamin D
Chem Biol Interact. 2024 Apr 6:110997. doi: 10.1016/j.cbi.2024.110997. Online ahead of print.ABSTRACTIt is generally accepted that low vitamin D (VD) levels are associated with a high prevalence factor for Inflammatory bowel disease(IBD). IBD patients have observed higher levels of lipopolysaccharide(LPS), ALT, and AST than healthy people. Gut-derived LPS causes inflammatory injury in the liver and kidney. The VD-metabolizing mechanism is involved in the liver and kidney, which means IBD might impact VD metabolism. However, whether IBD affects VD metabolism has not been studied. In vitro LPS resulted in decreased CYP2R1 in...
Source: Chemico-Biological Interactions - April 8, 2024 Category: Molecular Biology Authors: Yingyu Lu Hao Chen Yonger Chen Liang Zhao Shaozhen Hou Source Type: research
Effect of Antenatal Oral Vitamin D Supplementation on Serum 25(OH)D Concentration in Exclusively Breastfed Infants at 6 Months of age - A Randomized Double-Blind Placebo-Controlled Trial
CONCLUSIONS: Oral supplementation of vitamin D3 to pregnant women in the third trimester prevents severe hypovitaminosis D in the EBF infants at 6-months of age.PMID:38584410 (Source: Indian Pediatrics)
Source: Indian Pediatrics - April 8, 2024 Category: Pediatrics Authors: Prashant Prabhakar M M A Faridi Anju Aggarwal Rachna Aggarwal S V Madhu Rajeev Malhotra Source Type: research
Supplementation with vitamin D improves the embryo quality in in vitro fertilization (IVF) programs, independently of the patients ’ basal vitamin D status
ConclusionSupplementation of VD in patients undergoing ICSI procedures significantly improved the number of top-quality embryos compared with the control group, either starting from VD normal baseline values or starting from low values.Trial registration number07/2018. (Source: Archives of Gynecology and Obstetrics)
Source: Archives of Gynecology and Obstetrics - April 5, 2024 Category: OBGYN Source Type: research
Nutraceutically-enhanced oral delivery of vitamin D3 via Bio-SNEDDS: Demonstrating in vivo superiority over pediatric formulations
CONCLUSIONS: The Bio-SNEDDS provides an effective VD3 delivery strategy with established in vivo superiority over marketed products, along with offering additional health benefits from the natural oils.PMID:38574607 | DOI:10.1016/j.bbrc.2024.149852 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - April 4, 2024 Category: Biochemistry Authors: Mohsin Kazi Fahad Almarri Ahmad Abdul-Wahhab Shahba Ajaz Ahmad Salem Albraiki Fars Kaed Alanazi Source Type: research
Oral lichen planus among patients from Lublin Region in relation to 25-hydroxy-vitamin D3 serum level
CONCLUSIONS: The study enhances relationship between reduced levels of vitamin D3 and lichen planus in patients with oral lesions. Thus, vitamin D3 control and supplementation may play an important role in the treatment of lichen planus.PMID:38549488 | DOI:10.26444/aaem/169166 (Source: Annals of Agricultural and Environmental Medicine : AAEM)
Source: Annals of Agricultural and Environmental Medicine : AAEM - March 29, 2024 Category: Environmental Health Authors: Karolina Joanna Thum-Tyzo Bart łomiej Jacek Tyzo Renata Cha łas Source Type: research
The antimicrobial effect of different vitamin D compounds on < em > Streptococcus mutans < /em > and their impact on glycosyltransferase expression
CONCLUSION: Vitamin D2 and D3 inhibited S. mutans mediated pH drop in sucrose supplemented cultures and altered glycosyltransferase expression, suggesting potential therapeutic roles in dental caries prevention. Further research is needed to assess their full impact on S. mutans survival under environmental stresses.PMID:38550660 | PMC:PMC10976999 | DOI:10.1080/20002297.2024.2327758 (Source: Journal of Oral Microbiology)
Source: Journal of Oral Microbiology - March 29, 2024 Category: Microbiology Authors: Marta Picolo Abish Stephen Aylin Baysan Source Type: research
Oral lichen planus among patients from Lublin Region in relation to 25-hydroxy-vitamin D3 serum level
CONCLUSIONS: The study enhances relationship between reduced levels of vitamin D3 and lichen planus in patients with oral lesions. Thus, vitamin D3 control and supplementation may play an important role in the treatment of lichen planus.PMID:38549488 | DOI:10.26444/aaem/169166 (Source: Annals of Agricultural and Environmental Medicine : AAEM)
Source: Annals of Agricultural and Environmental Medicine : AAEM - March 29, 2024 Category: Environmental Health Authors: Karolina Joanna Thum-Tyzo Bart łomiej Jacek Tyzo Renata Cha łas Source Type: research
