Application of an endo-xylanase from Aspergillus japonicus in the fruit juice clarification and fruit peel waste hydrolysis
Publication date: Available online 28 August 2019Source: Biocatalysis and Agricultural BiotechnologyAuthor(s): Patricia Oliveira da Silva, Nelciele Cavalieri de Alencar Guimarães, John Dayvan Maidana Serpa, Douglas Chodi Masui, Clarice Rossatto Marchetti, Newton Valério Verbisck, Fabiana Fonseca Zanoelo, Roberto Ruller, Giovana Cristina GiannesiAbstractThe endo-xylanase from Aspergillus japonicus (UFMS 48.136) was purified in a single step using carboximethyl-cellulose chromatographic column and applied in fruit juice clarification process and fruit peel waste hydrolysis. This purification procedure resulted in 38.9-fold...
Source: Biocatalysis and Agricultural Biotechnology - August 29, 2019 Category: Biotechnology Source Type: research
Determination of ascorbic acid in natural fruit juices using digital image colorimetry
Publication date: September 2019Source: Microchemical Journal, Volume 149Author(s): Icaro S.A. Porto, Joao H. Santos Neto, Liz O. dos Santos, Adriano A. Gomes, Sergio L.C. FerreiraAbstractThis work proposes a procedure using digital image colorimetry for the determination of ascorbic acid in natural fruit juices. The method was established by measuring the color of the iron(II)-1,2 ortho-phenanthroline complex. The digital images obtained during the experiments were stored in JPEG format, and the region of interest (ROI) was submitted to decomposition in color values according to the RGB additive color model. However, the ...
Source: Microchemical Journal - June 30, 2019 Category: Chemistry Source Type: research
Identification of Novel Endophytic Yeast Strains from Tangerine Peel.
Abstract
Seven endophytic yeast strains were isolated from tangerine peel (Citrus reticulata Blanco) and genotyped through clustering with D1/D2 and ITS1-5.8S-ITS2 sequences from GenBank. Phenotypic characteristics were obtained through commercial kits and through assisted species identification. Indole-3-acetic acid (IAA) production by the yeast strains was assessed using Salkowski reagent and High-Performance Liquid chromatography (HPLC). The growth-promoting effects of the yeast were evaluated using the 'ragdoll' method. CRYb1, CRYb2 and CRYb7 isolates were identified as the closest species Hanseniaspor...
Source: Current Microbiology - June 25, 2019 Category: Microbiology Authors: Ling L, Li Z, Jiao Z, Zhang X, Ma W, Feng J, Zhang J, Lu L Tags: Curr Microbiol Source Type: research
Cellulomonas aurantiaca sp. nov., isolated from a soil sample from a tangerine field.
Abstract
A Gram-stain positive, facultatively aerobic, motile and rod-shaped bacterial strain, designated THG-SMD2.3T, was isolated from a soil sample collected in a tangerine field, Republic of Korea. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Cellulomonas and to be closely related to Cellulomonas fimi ATCC 484T (98.5%), Cellulomonas biazotea DSM 20112T (98.3%), Cellulomonas chitinilytica X.bu-bT (98.0%), Cellulomonas xylanilytica XIL11T (97.2%), Cellulomonas humilata ATCC 25174T (97.1%) and Cellulomonas composti TR7-06T (97.0%). The 16S rRNA g...
Source: Antonie van Leeuwenhoek - June 18, 2019 Category: Microbiology Authors: Kim SK, Kook M, Yan ZF, Trinh H, Zheng SD, Yang JE, Park SY, Yi TH Tags: Antonie Van Leeuwenhoek Source Type: research
Modeling inactivation of Listeria monocytogenes, Shigella sonnei, Byssochlamys fulva and Saccharomyces cerevisiae and ascorbic acid and β-carotene degradation kinetics in tangerine juice by pulsed-thermosonication
Publication date: Available online 13 May 2019Source: LWTAuthor(s): Seyed Mohammad Bagher Hashemi, Reza Roohi, Mohammad Reza Mahmoudi, Daniel GranatoAbstractInactivation of Listeria monocytogenes (serotype 4 ab), Shigella sonnei, Byssochlamys fulva and Saccharomyces cerevisiae and the degradation of β-carotene and ascorbic acid in tangerine juice subjected to pulsed-thermosonication were modeled using polynomial, log-logistic, Weibull, biphasic linear and modified Gompertz models. The models were assessed using the adjusted determination coefficient (adj-R2) and the root mean square error (RMSE). Then, Ward's clustering...
Source: LWT Food Science and Technology - May 15, 2019 Category: Food Science Source Type: research
Shaddock peels-based activated carbon as cost-saving adsorbents for efficient removal of Cr (VI) and methyl orange.
Abstract
A simple and economical method was proposed to synthesize the shaddock peels-based activated carbon (SPAC) for their application as efficient sorbents to eliminate Cr (VI) and methyl orange (MO) from one-component and two-component systems. The synthesis was conducted via activation of phosphoric acid and high-temperature carbonization. The as-prepared SPAC was characterized by Brunauer-Emmett-Teller, scanning electron microscopy, energy dispersive X-ray spectroscopy, thermogravimetric analysis, X-ray diffraction, and Fourier transform infrared spectroscopy, among other techniques. The adsorption ...
Source: Environmental Science and Pollution Research International - May 14, 2019 Category: Environmental Health Authors: Tao X, Wu Y, Cha L Tags: Environ Sci Pollut Res Int Source Type: research
In vitro Susceptibility and Evaluation of Techniques for Understanding the Mode of Action of a Promising Non-antibiotic Citrus Fruit Extract Against Several Pathogens
Conclusion
This study confirms the in vitro antibacterial activity of BIOCITRO® against Gram-negative and Gram-positive bacteria. For most of the strains, the product reached the bactericidal effect at the same concentration of the bacteriostatic effect and maximum difference between MIC and MBC was two dilution steps. The less susceptible species of the study were S. enterica ssp. enterica and E. coli with MBC90 values of 256 and 128 μg/mL, respectively, while the most susceptible was C. perfringens with MBC90 of 16 μg/mL. After short exposition time to the product, the significant effect over the vi...
Source: Frontiers in Microbiology - April 23, 2019 Category: Microbiology Source Type: research
Chitinophaga aurantiaca sp. nov., isolated from a soil sample from a tangerine field.
Abstract
A Gram-stain-negative, facultatively anaerobic, non-motile and rod-shaped bacterial strain, designated THG-SD5.5T, was isolated from a soil sample collected in a tangerine field, Republic of Korea. According to the 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus Chitinophaga and to be closely related to Chitinophaga ginsengihumi KACC 17604T (97.9%) and Chitinophaga rupis KACC 14521T (97.5%). The 16S rRNA gene sequence similarities with other species of the genus Chitinophaga were in the range 92.8-95.5%. Catalase test was positive. Oxidase test was negative....
Source: Antonie van Leeuwenhoek - March 27, 2019 Category: Microbiology Authors: Kim SK, Kook M, Yan ZF, Park SY, Kim SS, Kim HB, Trinh H, Won KH, Yang JE, Yi TH Tags: Antonie Van Leeuwenhoek Source Type: research
Efficiency of four different dietary preparation methods in extracting functional compounds from dried tangerine peel
In this study, the efficiency of four different dietary preparation methods (i.e. soaking, boiling, steaming, and ethanol extraction) in extraction of functional compounds (i.e. flavonoids and essential oil constituents) from DTP was evaluated systematically for the first time. To conduct a comprehensive evaluation of the extraction of the functional compounds, a synthetic evaluation model based on a weighting method was established. The optimum conditions of each dietary preparation method (e.g., time, temperature, solid-liquid ratio, etc.) were determined by response surface methodology. Ethanol extraction showed the bes...
Source: Food Chemistry - March 14, 2019 Category: Food Science Source Type: research
1H NMR-based metabolomics profiling of citrus juices produced in Veracruz, M éxico.
This study describes the 1H NMR-based metabolomics profiling of juices from citrus fruits harvested in the state of Veracruz, México. The hydrophilic profile of commercial lemons (Agrio and Persian), tangerines (Fremont and Mónica), oranges (Valencia and Washington Navel), and grapefruits (Red Ruby and Rio Red) was determined. According to our results, 35 metabolites were identified in the 1H NMR profiling. The statistical differences obtained by PCA and OPLS-DA revealed that specific amino acids, sugars, and organic acids were differential metabolites in the species and cultivars studied. High endogenous levels of sucro...
Source: Chemistry and Biodiversity - February 26, 2019 Category: Biochemistry Authors: Villa-Ruano N, Pérez-Hernández N, Zepeda-Vallejo LG, Quiroz-Acosta T, Mendieta-Moctezuma A, Montoya-García CO, García-Nava ML, Becerra Martínez E Tags: Chem Biodivers Source Type: research
Seasonal variation and detection frequency of Candidatus Liberibacter asiaticus in Binchuan, Yunnan province China
In this study, we investigated the seasonal variations in the population dynamics of Clas and assessed the possible genetic variations among the identified isolates. We conducted a disease survey in tangerine citrus groves affected with HLB in Binchuan county, Yunnan province, China, on a monthly basis over a period of one year. The collected leaf samples were analyzed every month via nested PCR, targeting the 16S rRNA and the β-operon ribosomal protein-encoding genes of Clas. The abundance of the HLB-causing pathogen changes several times, with high temperatures in July 2017 yielding low pathogen abundance, followed by a...
Source: Physiological and Molecular Plant Pathology - February 1, 2019 Category: Molecular Biology Source Type: research
The methionine biosynthesis regulator AaMetR contributes to oxidative stress tolerance and virulence in Alternaria alternata
In conclusion, our results highlight the global regulatory role of AaMetR in cysteine and methionine metabolism and provide new insights into the crucial role of ROS detoxification, sporulation and pathogenicity in the tangerine pathotype of A. alternata. (Source: Microbiological Research)
Source: Microbiological Research - November 24, 2018 Category: Infectious Diseases Source Type: research
Urine can speed up the re-epithelialization process of prostatic urethra wounds by promoting the proliferation and migration of prostate epithelial cells
ConclusionUrine may speed up the re-epithelialization process for prostatic urethra wounds by promoting proliferation and migration of prostate epithelial cells. (Source: International Urology and Nephrology)
Source: International Urology and Nephrology - November 7, 2018 Category: Urology & Nephrology Source Type: research
Molecules, Vol. 23, Pages 2660: Simultaneous Separation and Purification of Five Polymethoxylated Flavones from “Dahongpao” Tangerine (Citrus tangerina Tanaka) Using Macroporous Adsorptive Resins Combined with Prep-HPLC
In this study, a preparative separation method was established to simultaneously isolate the polymethoxylated flavones (PMFs) from the peel of “Dahongpao” tangerine using macroporous adsorptive resins (MARs) combined with prep-HPLC. The total PMFs were enriched using MARs to remove most sugars, water-soluble pigments, and flavanones, and the eluents obtained were analyzed by ultra-performance liquid chromatography (UPLC) to determine the PMF composition. The separation and purification of PMFs were carried out by using a mass spectrometry-guided prep-HPLC with a gradient elution of acetonitrile-...
Source: Molecules - October 16, 2018 Category: Chemistry Authors: Zhenqing Li Ziyan Zhao Zhiqin Zhou Tags: Article Source Type: research
Bulk industrial fruit fibres. Characterization and prevalence of the original fruit metabolites
Publication date: September 2018 Source:Food Research International, Volume 111 Author(s): Antoni Delpino-Rius, Alexis Marsol-Vall, Jordi Eras, Montse Llovera, Miguel Ángel Cubero, Mercè Balcells, Ramon Canela-Garayoa Here we analysed the content of primary and secondary metabolites in nine types of industrially processed fibres derived from the juice industry. Specifically, we examined fibre from: apple, peach, and pear, as non-citrus fruits; the peel and flesh of orange and tangerine, and lemon flesh, as citrus fruits; and carrot, as vegetable. Regarding primary metabolites, the sugar content ranged from 21.6...
Source: Food Research International - May 17, 2018 Category: Food Science Source Type: research
