Comparative genomics of Lactobacillus sakei supports the development of starter strain combinations
Publication date: Available online 14 January 2019Source: Microbiological ResearchAuthor(s): Lara Eisenbach, Andreas J. Geissler, Matthias A. Ehrmann, Rudi F. VogelAbstractStrains of Lactobacillus sakei can be isolated from a variety of sources including meat, fermented sausages, sake, sourdough, sauerkraut or kimchi. Selected strains are widely used as starter cultures for sausage fermentation. Recently we have demonstrated that control about the lactic microbiota in fermenting sausages is achieved rather by pairs or strain sets than by single strains. In this work we characterized the pan genome of L. sakei to enable exp...
Source: Microbiological Research - January 15, 2019 Category: Infectious Diseases Source Type: research

Differential involvement of glycans in the binding of Staphylococcus epidermidis and Corynebacterium spp. to human sweat
Publication date: Available online 30 December 2018Source: Microbiological ResearchAuthor(s): Chi-Hung Lin, Robyn A Peterson, Audrey Gueniche, Ségolène Adam de Beaumais, Virginie Hourblin, Lionel Breton, Maria Dalko, Nicolle H. PackerAbstractSweat is a secretory fluid that can be a source of unpleasant body odour due to interaction of resident bacteria with sweat components. Identification of glycoproteins in sweat suggests that protein-conjugated glycans may act as binding epitopes for bacteria, as found in other secretory fluids such as human milk, tears and saliva which help to protect epithelial surfaces ...
Source: Microbiological Research - December 30, 2018 Category: Infectious Diseases Source Type: research

Differential gene expression in two grapevine cultivars recovered from “flavescence dorée”
Publication date: Available online 28 December 2018Source: Microbiological ResearchAuthor(s): Davide Pacifico, Paolo Margaria, Luciana Galetto, Mauro Legovich, Simona Abbà, Flavio Veratti, Cristina Marzachì, Sabrina PalmanoAbstractThe biological bases of recovery of two grapevine cultivars, Nebbiolo and Barbera, showing different susceptibility and recovery ability to “flavescence dorée” (FD) phytoplasma infection were investigated. The expression over one vegetative season, in FD-recovered and healthy grapevines, of 18 genes involved in defence, hydrogen peroxide and hormone production was...
Source: Microbiological Research - December 29, 2018 Category: Infectious Diseases Source Type: research

Sestrin-like protein from Dictyostelium discoideum is involved in autophagy under starvation stress
Publication date: Available online 28 December 2018Source: Microbiological ResearchAuthor(s): S Rafia, S SaranAbstractSestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukaryote where starvation stress initiates multicellular development. The single DdSesn-like gene was expressed and its endogenous functions were characterized. Both, the knockout and constitutively...
Source: Microbiological Research - December 29, 2018 Category: Infectious Diseases Source Type: research

Reactive green dye remediation by Alternanthera philoxeroides in association with plant growth promoting Klebsiella sp. VITAJ23; A pot culture study
Publication date: Available online 21 December 2018Source: Microbiological ResearchAuthor(s): Astha Sinha, Sajitha Lulu S, Vino S, W.Jabez OsborneAbstractContamination of soil by textile effluent is a major threat found worldwide. These pollutants have a diverse negative effects on the ecosystem, therefore restoration through a cost biological strategy is the need of the hour. The aim of the current study was to enhance the decolourization of reactive green dye (RGD) by phytoremediation coupled with augmentation of effective bacteria to the rhizosphere. The isolate Klebsiella sp. VITAJ23 was isolated from textile polluted ...
Source: Microbiological Research - December 22, 2018 Category: Infectious Diseases Source Type: research

Specific regions of the SulA protein recognized and degraded by the ATP-dependent ClpYQ (HslUV) protease in Escherichia coli
In this study, using yeast two-hybrid and in vivo degradation analyses, we first demonstrated that the C-terminal internal hydrophobic region (139th~ 149th aa) of SulA is necessary for binding and degradation by ClpYQ. A conserved region, GFIMRP, between 142th and 147th residues of SulA, were identified among various Gram-negative bacteria. By using MBP-SulA(F143Y) (phenylalanine substituted with tyrosine) as a substrate, our results showed that this conserved residue of SulA is necessary for recognition and degradation by ClpYQ. Supporting these data, MBP-SulA(F143Y), MBP-SulA(F143 N) (phenylalanine substituted wit...
Source: Microbiological Research - December 18, 2018 Category: Infectious Diseases Source Type: research

The stringent response factor, RelA, positively regulates T6SS4 expression through the RovM/RovA pathway in Yersinia pseudotuberculosis
Publication date: Available online 6 December 2018Source: Microbiological ResearchAuthor(s): Xiaobing Yang, Yunhong Song, Qingyun Dai, Hongyun Zhang, Li Song, Zhuo Wang, Junfeng Pan, Yao WangAbstractThe type VI secretion system (T6SS) is a versatile molecular machinery widely distributed in Gram-negative bacteria. The activity of the T6SS is tightly regulated by various mechanisms, including quorum sensing (QS), iron concentration, and transcriptional regulators. Here we demonstrated that the stringent response regulator, RelA, contributes to bacterial resistance to multiple environmental stresses in Yersinia pseudotubercu...
Source: Microbiological Research - December 7, 2018 Category: Infectious Diseases Source Type: research

Control of Fusarium wilt of lisianthus by reassembling the microbial community in infested soil through reductive soil disinfestation
In this study, a pre-cultivation soil management strategy, reductive soil disinfestation (RSD), involving amendment by the use of labile organic substrates, such as reed straw, bagasse, and rice straw, and creating anaerobic soil conditions, was used to regulate the microbial community in a soil infested by Fusarium wilt of lisianthus and make it suitable for plant cultivation. The results showed that RSD significantly decreased F. oxysporum population by 97.1%–99.1% and the incidence of lisianthus wilt disease to 3.0%–14.3% compared with that of the untreated soil. The lowest disease incidence was found in the...
Source: Microbiological Research - December 7, 2018 Category: Infectious Diseases Source Type: research

Avocado rhizobacteria emit volatile organic compounds with antifungal activity against Fusarium solani, Fusarium sp. associated with Kuroshio shot hole borer, and Colletotrichum gloeosporioides
Publication date: Available online 24 November 2018Source: Microbiological ResearchAuthor(s): Edgar Guevara-Avendaño, Alix Adriana Bejarano-Bolívar, Ana-Luisa Kiel-Martínez, Mónica Ramírez-Vázquez, Alfonso Méndez-Bravo, Eneas Aguirre von Wobeser, Diana Sánchez-Rangel, José A. Guerrero-Analco, Akif Eskalen, Frédérique ReverchonAbstractRecent studies showed that bacterial volatile organic compounds (VOCs) play an important role in the suppression of phytopathogens. The ability of VOCs produced by avocado (Persea americana Mill.) rhizobacteria to sup...
Source: Microbiological Research - November 25, 2018 Category: Infectious Diseases Source Type: research

MarTrack: A versatile toolbox of mariner transposon derivatives used for functional genetic analysis of bacterial genomes
In this study, we combined the merits of the random mutagenesis of mariner transposon and common efficient reporter marker genes or regulatory elements, mcherry, gfp, luxAB, lacZ, sacBR, and PBAD and antibiotic resistance cassettes to construct a series of derivative transposon vectors, pMmch, pMKGR, pMCGR, pMXKGR, pMLKGR, pMSGR, and pMPR, based on the initial transposon pMar2xT7. The function and effectiveness of the modified transposons were verified by introducing them into E. piscicida EIB202. Based on the toolbox, a transposon insertion mutant library containing approximately 3.0 × 105 separated mu...
Source: Microbiological Research - November 24, 2018 Category: Infectious Diseases Source Type: research

Pseudomonas protegens MP12: A plant growth-promoting endophytic bacterium with broad-spectrum antifungal activity against grapevine phytopathogens
Publication date: Available online 23 November 2018Source: Microbiological ResearchAuthor(s): Marco Andreolli, Giacomo Zapparoli, Elisa Angelini, Gianluca Lucchetta, Silvia Lampis, Giovanni ValliniAbstractPseudomonas sp. MP12 was isolated from a soil sample collected in a typical warm-temperate deciduous forest near Brescia, northern Italy. Phylogenetic analysis identified the species as Pseudomonas protegens. We detected the genes phlD, pltB and prnC, which are required to synthesize the antifungal compounds 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin and pyrrolnitrin, respectively. P. protegens MP12 was also shown...
Source: Microbiological Research - November 24, 2018 Category: Infectious Diseases Source Type: research

Interactions between Pseudomonas spp. and their role in improving the red pepper plant growth under salinity stress
Publication date: Available online 23 November 2018Source: Microbiological ResearchAuthor(s): Sandipan Samaddar, Poulami Chatterjee, Aritra Roy Choudhury, Shamim Ahmed, Tongmin SaAbstractSolitary inoculation of bacteria has been studied widely for plant growth development and amelioration of salinity stress but co-inoculation of bacteria for salt stress amelioration in red pepper plants has been less studied till date. Here, we investigated the co-inoculation effect of Pseudomonas frederiksbergensis OB139 and Pseudomonas vancouverensis OB155 in red pepper plant growth characteristics, plant photosynthesis pigments, ethylen...
Source: Microbiological Research - November 24, 2018 Category: Infectious Diseases Source Type: research

The methionine biosynthesis regulator AaMetR contributes to oxidative stress tolerance and virulence in Alternaria alternata
In conclusion, our results highlight the global regulatory role of AaMetR in cysteine and methionine metabolism and provide new insights into the crucial role of ROS detoxification, sporulation and pathogenicity in the tangerine pathotype of A. alternata. (Source: Microbiological Research)
Source: Microbiological Research - November 24, 2018 Category: Infectious Diseases Source Type: research

Application of rice (Oryza sativa L.) root endophytic diazotrophic Azotobacter sp. strain Avi2 (MCC 3432) can increase rice yield under green house and field condition
Publication date: Available online 23 November 2018Source: Microbiological ResearchAuthor(s): Avishek Banik, Gautam Kumar Dash, Padmini Swain, Upendra Kumar, Subhra Kanti Mukhopadhyay, Tushar Kanti DangarAbstractUse of plant-associated beneficial microbes, especially endophytes are getting popular day by day as they occupy a relatively privileged niche inside different plant tissues with lesser competition for food and shelter than rhizosphere. The effects of different physical factors like temperature, rainfall, and seasonal variation and UV radiation on plant growth promoting endophytic communities are less pronounced th...
Source: Microbiological Research - November 24, 2018 Category: Infectious Diseases Source Type: research

Screening, plant growth promotion and root colonization pattern of two rhizobacteria (Pseudomonas fluorescens Ps006 and Bacillus amyloliquefaciens Bs006) on banana c.v Williams (Musa acuminata)
Publication date: Available online 23 November 2018Source: Microbiological ResearchAuthor(s): R. Gamez, M. Cardinale, M. Montes, S. Ramirez, S. Schnell, F. RodriguezAbstractBanana is the second largest export crop in Colombia. To meet the demand of international markets, high amounts of chemical fertilizers are required, which represent high costs and can be hazardous to the environment. Plan growth promoting rhizobacteria (PGPR) can at least partially replace chemical fertilizers. In this paper, we evaluated the effect of nine PGPR of the genera Bacillus and Pseudomonas on Banana growth. Banana seedlings were produced thr...
Source: Microbiological Research - November 23, 2018 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: January 2019Source: Microbiological Research, Volume 218Author(s): (Source: Microbiological Research)
Source: Microbiological Research - November 17, 2018 Category: Infectious Diseases Source Type: research

Retraction notice to "The opportunistic human fungal pathogen Candida albicans promotes the growth and proliferation of commensal Escherichia coli through an iron-responsive pathway" [Microbiological Research 207 (2018) 232-239]
Publication date: January 2019Source: Microbiological Research, Volume 218Author(s): Shanshan Li, Xiaoyu Yu, Wenjuan Wu, Daniel Z. Chen, Ming Xiao, Xinhua Huang (Source: Microbiological Research)
Source: Microbiological Research - November 17, 2018 Category: Infectious Diseases Source Type: research

Bacterial chemotaxis coupling protein: Structure, function and diversity
Publication date: Available online 6 November 2018Source: Microbiological ResearchAuthor(s): Zhiwei Huang, Xiaoyue Pan, Nan Xu, Minliang GuoAbstractIn most signal transduction systems, coupling or scaffold proteins establish crucial connections between receptors and histidine kinases. These connections are important for signal transduction. The bacterial chemotaxis system is a canonical signal transduction system that relies on coupling proteins. The coupling proteins in the chemotaxis system have two architectures: CheW or CheV. In a typical chemotaxis signal transduction system, two CheW coupling protein molecules bridge...
Source: Microbiological Research - November 7, 2018 Category: Infectious Diseases Source Type: research

Physiological response of Lactuca sativa exposed to 2-nonanone emitted by Bacillus sp. BCT9
In this study, 2-day-old L. sativa were exposed to 2-nonanone for 10 days under two delivery systems: 1) 2-nonanone (abrupt delivery) and 2) 2-nonanone + lanolin (controlled delivery). The X-ray elemental microanalysis, scanning electron and confocal laser microscopies techniques were used to evaluate physiological changes “in vivo” conditions. The results indicated that 2-nonanone increased root and shoot length independently of 2-nonanone delivery system after 7 days of exposition. Additionally, 2-nonanone elicited the increase of anthocyanin and not affects chlorophyll content and electrolyte l...
Source: Microbiological Research - November 4, 2018 Category: Infectious Diseases Source Type: research

Development of low-cost formulations of plant growth-promoting bacteria to be used as inoculants in beneficial agricultural technologies
Publication date: Available online 2 November 2018Source: Microbiological ResearchAuthor(s): Constanza Belén Lobo, María Silvina Juárez Tomás, Emilce Viruel, Marcela Alejandra Ferrero, María Ester LuccaAbstractPhosphorus is one of the main macronutrients for plant development. Despite its large deposits in soils, it is scarcely available for plants. Phosphate-solubilizing bacteria, belonging to the group of plant growth-promoting rhizobacteria (PGPR), are capable of mobilizing deposits of insoluble phosphates in the soil. The use of PGPR as inoculants provides an environmentally sustainab...
Source: Microbiological Research - November 3, 2018 Category: Infectious Diseases Source Type: research

Current situation of biofuel production and its enhancement by CRISPR/Cas9-mediated genome engineering of microbial cells
Publication date: Available online 2 November 2018Source: Microbiological ResearchAuthor(s): Muhammad Rizwan Javed, Muhammad Noman, Muhammad Shahid, Temoor Ahmed, Mohsin Khurshid, Muhammad Hamid Rashid, Muhammad Ismail, Maria Sadaf, Fahad KhanAbstractGeopolitical and economic factors have motivated the scientific community to utilize renewable energy resources. In addition to the modifications in major steps and processes of biofuel production, manipulation of microbial genome engineering tools is essential in order to find sustainable solution of continuous depletion of fossil-fuels. Recently, the CRISPR (clustered regula...
Source: Microbiological Research - November 3, 2018 Category: Infectious Diseases Source Type: research

The Potential of Bacilli Rhizobacteria for Sustainable Crop Production and Environmental Sustainability
Publication date: Available online 2 November 2018Source: Microbiological ResearchAuthor(s): B.N. Aloo, B.A. Makumba, E.R. MbegaAbstractConventional agricultural practices often rely on synthetic fertilizers and pesticides which have immense and adverse effects on humans, animals and environments. To minimize these effects, scientists world over are now deeply engaged in finding alternative approached for crop production which are less dependent on chemical inputs. One such approach is the use of rhizospheric bacteria as vital components of soil fertility and plant growth promotion (PGP) through their direct and indirect p...
Source: Microbiological Research - November 3, 2018 Category: Infectious Diseases Source Type: research

Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses
In this study, we investigated the influence of the deletion of all active Tat pathway components on the E. coli cells. The comprehensive proteomic analysis revealed activation of several stress responses and experimentally confirmed the dependence of certain proteins on the Tat system for export. We observed that a tat deletion triggers protein aggregation, membrane vesiculation, synthesis of colanic acid and biofilm formation. Furthermore, the mislocalization of Tat-dependent proteins disturbs iron and molybdenum homeostasis and impairs the cell envelope integrity. The results show that the functional Tat pathway is impo...
Source: Microbiological Research - November 2, 2018 Category: Infectious Diseases Source Type: research

Transcriptome analysis of hopanoid deficient mutant of Rhodopseuodomonas palustris TIE-1
Publication date: Available online 29 October 2018Source: Microbiological ResearchAuthor(s): Tushar D. Lodha, Indu B., Sasikala Ch., Ramana Ch.V.AbstractAll three domains of life have an ordered plasma membrane which is pivotal in the selective fitness of primitive life. Like cholesterol in eukaryotes, hopanoids are important in bacteria to modulate membrane order. Hopanoids are pentacyclic triterpenoid lipids biosynthesised in many eubacteria, few ferns and lichens. Hopanoid modulates outer membrane order and hopanoid deficiency results in the weakened structural integrity of the membrane which may intern affect the other...
Source: Microbiological Research - October 30, 2018 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: December 2018Source: Microbiological Research, Volume 217Author(s): (Source: Microbiological Research)
Source: Microbiological Research - October 30, 2018 Category: Infectious Diseases Source Type: research

The histidine kinase slnCl1 of Colletotrichum lindemuthianum as a pathogenicity factor against Phaseolus vulgaris L
Publication date: Available online 28 October 2018Source: Microbiological ResearchAuthor(s): Guilherme Bicalho Nogueira, Leandro Vieira dos Santos, Casley Borges de Queiroz, Thamy Lívia Ribeiro Corrêa, Renato Pedrozo Menicucci, Denise Mara Soares Bazzolli, Elza Fernandes de Araújo, Marisa Vieira de QueirozAbstractColletotrichum lindemuthianum, the causal agent of anthracnose, is responsible for significant damage in the common bean (Phaseolus vulgaris L.). Unraveling the genetic mechanisms involved in the plant/pathogen interaction is a powerful approach for devising efficient methods to control this di...
Source: Microbiological Research - October 29, 2018 Category: Infectious Diseases Source Type: research

Plant-microbe interactions endorse growth by uplifting microbial community structure of Bacopa monnieri rhizosphere under nematode stress
Publication date: Available online 26 October 2018Source: Microbiological ResearchAuthor(s): Rupali Gupta, Akanksha Singh, Madhumita Srivastava, Karuna Shanker, Rakesh PandeyAbstractThe modification of rhizosphere microbial diversity and ecological processes are of rising interest as shifting in microbial community structure impacts the mutual role of host–microbe interactions. Nevertheless, the connection between host-microbial community diversity, their function under biotic stress in addition to their impact on plant performances is poorly understood. The study was designed with the aim to analyze the tripartite i...
Source: Microbiological Research - October 27, 2018 Category: Infectious Diseases Source Type: research

Deletion of the epigenetic regulator GcnE in Aspergillus niger FGSC A1279 activates the production of multiple polyketide metabolites
This study found that inactivation of GcnE activated the production of secondary metabolites in A. niger. The biosynthetic pathway for nigerpyrone and its derivatives was identified and characterized via gene knockout and complementation experiments. A biosynthetic model of this group of pyran-based fungal metabolites was proposed. (Source: Microbiological Research)
Source: Microbiological Research - October 16, 2018 Category: Infectious Diseases Source Type: research

Corrigendum to “Streptomyces puniceus strain AS13., Production, characterization and evaluation of bioactive metabolites: A new face of dinactin as an antitumor antibiotic” [Microbiological Research 207, (2018)196-202]
Publication date: Available online 9 October 2018Source: Microbiological ResearchAuthor(s): Aehtesham Hussain, Muzafar Ahmad Rather, Mohd Saleem Dar, N A Dangroo, Mushtaq A. Aga, Arem qayum, Aabid Manzoor Shah, Zahoor Ahmad, Mohd Jamal Dar, Qazi Parvaiz Hassan (Source: Microbiological Research)
Source: Microbiological Research - October 10, 2018 Category: Infectious Diseases Source Type: research

Partner-triggered proteome changes in the cell wall of Bacillus sonorensis and roots of groundnut benefit each other
Publication date: Available online 9 October 2018Source: Microbiological ResearchAuthor(s): Sravani Ankati, T. Swaroopa Rani, Appa Rao PodileAbstractPlant growth promoting rhizobacteria (PGPR) promote plant growth and activate defense response against phytopathogens. At the subcellular level plant-PGPR interaction is less understood, which would be essential for future improvement(s) of PGPR formulations. In a rigorous screening process, that also involved efficient PGPR strains, Bacillus sonorensis RS4 was selected to study partner-triggered interactions. The potential of B. sonorensis RS4 to improve growth of groundnut, ...
Source: Microbiological Research - October 10, 2018 Category: Infectious Diseases Source Type: research

Antimicrobial, plant growth-promoting and genomic properties of the peanut endophyte Bacillus velezensis LDO2
In this study, the peanut endophytic bacterium LDO2 was evaluated for the potential of peanut pathogens inhibition and growth-promotion, and the genetic mechanisms were explored by genome mining. Strain LDO2 significantly inhibited the growth of peanut pathogenic fungi and pathogenic bacteria, and specifically, it showed pronounced inhibition on mycelia growth of Aspergillus flavus mycelia and caused mycelial deformity. Gene clusters responsible for antifungal metabolites (fengycin, surfactin, bacilysin) and antibacterial metabolites (butirosin, bacillaene, difficidin, macrolactin, surfactin, bacilysin) were identified. St...
Source: Microbiological Research - October 10, 2018 Category: Infectious Diseases Source Type: research

Isolation and characterization of endophytes from nodules of Mimosa pudica with biotechnological potential
Publication date: Available online 6 October 2018Source: Microbiological ResearchAuthor(s): Ricardo Sánchez-Cruz, Irán Tapia Vázquez, Ramón Alberto Batista-García, Erick Williams Méndez Santiago, María del Rayo Sánchez Carbente, Alfonso Leija, Verónica Lira-Ruan, Gerogina Hernández, Arnoldo Wong-Villarreal, Jorge Luis Folch-MallolAbstractLegumes establish symbiotic relationships with different microorganisms, which could function as plant growth promotion microorganisms (PGPM). The finding of new PGPM strains is important to increase plant production avo...
Source: Microbiological Research - October 7, 2018 Category: Infectious Diseases Source Type: research

Environmental conditions shape the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125
Publication date: Available online 6 October 2018Source: Microbiological ResearchAuthor(s): Annarita Ricciardelli, Angela Casillo, Alessandro Vergara, Nicole Balasco, Maria Michela Corsaro, Maria Luisa Tutino, Ermenegilda ParrilliAbstractBiofilms are the most widely distributed and successful microbial modes of life. The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress. Bacteria living in cold environments, like Antarctica, can be found as biofilms, even though the mechanisms o...
Source: Microbiological Research - October 7, 2018 Category: Infectious Diseases Source Type: research

Recombineering Pseudomonas protegens CHA0: An innovative approach that improves nitrogen fixation with impressive bactericidal potency
Publication date: Available online 6 October 2018Source: Microbiological ResearchAuthor(s): Fangnan Yu, Xiaoshu Jing, Xiaochen Li, Hailong Wang, Hanna Chen, Lin Zhong, Jia Yin, Deng Pan, Yulong Yin, Jun Fu, Liqiu Xia, Xiaoying Bian, Qiang Tu, Youming ZhangAbstractPseudomonas protegens CHA0 is a well-characterized, root-colonizing bacterium with broad-spectrum biocontrol ability. Therefore, it has a great potential to curb plant diseases and to partly replace synthetic chemical pesticides that are harmful to humans. Here, we obtained the multifunctional mutant CHA0-ΔretS-Nif via Red/ET recombineering technology. After...
Source: Microbiological Research - October 7, 2018 Category: Infectious Diseases Source Type: research

Helicobacter pylori evasion strategies of the host innate and adaptive immune responses to survive and develop gastrointestinal disease
Publication date: Available online 6 October 2018Source: Microbiological ResearchAuthor(s): Ahmad Karkhah, Soheil Ebrahimpour, Maryam Rostamtabar, Veerendra Koppolu, Sorena Darvish, Veneela Krishna Rekha Vasigala, Majid Validi, Hamid Reza NouriAbstractHelicobacter pylori (H. pylori) is a bacterial pathogen that resides in more than half of the human population and has co-evolved with humans for more than 58000 years. This bacterium is orally transmitted during childhood and is a key cause of chronic gastritis, peptic ulcers and two malignant cancers including MALT (mucosa-associated lymphoid tissue) lymphoma and adenocarci...
Source: Microbiological Research - October 6, 2018 Category: Infectious Diseases Source Type: research

Organization of multi-binding to host proteins: The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Mycoplasma pneumoniae
Publication date: January 2019Source: Microbiological Research, Volume 218Author(s): Juliane Grimmer, Roger DumkeAbstractMycoplasma pneumoniae is a frequent cause of community-acquired infections of the human respiratory tract. During the evolutionary adaptation of the bacteria to the host, the genome of the pathogen is strongly reduced resulting in the loss of cell wall, limited metabolic pathways and a relatively small number of virulence factors. As interacting with host proteins, surface-exposed proteins with a primary function in cytosol-located processes of metabolism and regulation such as glycolytic enzymes, heat-s...
Source: Microbiological Research - October 5, 2018 Category: Infectious Diseases Source Type: research

Genome sequence and comparative genomics of Rhizobium sp. Td3, a novel plant growth promoting phosphate solubilizing Cajanus cajan symbiont
Publication date: January 2019Source: Microbiological Research, Volume 218Author(s): Bhagya Iyer, Shalini RajkumarAbstractRhizobium sp. Td3 is a Sesbania plant growth promoting, Cajanus cajan nodulating rhizobia. Studying its whole genome was important as it is a potent phosphate solubilizer with constitutive gluconic acid production ability through operation of the periplasmic glucose oxidation pathway even under conditions of catabolite repression. This is in contrast to the other explored phosphate solubilizers. Rhizobial isolates sequenced so far are known to lack components of the direct glucose oxidation pathway and ...
Source: Microbiological Research - October 5, 2018 Category: Infectious Diseases Source Type: research

Cordycepin and Pentostatin Biosynthesis Gene Identified through Transcriptome and Proteomics Analysis of Cordyceps kyushuensis Kob
This study first sequenced transcriptome and proteomics of C. kyushuensis, and demonstrated that there is a single gene cluster related to biosynthesis of cordycepin and pentostatin, which can be employed to improve the yield of cordycepin and find more functional proteins. (Source: Microbiological Research)
Source: Microbiological Research - September 19, 2018 Category: Infectious Diseases Source Type: research

Enhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systems
In this study, fluorescent in situ hybridization (FISH) and catalyzed reporter deposition–FISH (CARD–FISH) were evaluated to determine the colonization ability of B. subtilis EA-CB0575 on banana and tomato roots planted on solid and liquid Murashige and Skoog medium (MS(S) and MS(L), respectively) and in soil for tomato plants. Results showed B. subtilis colonization 0–30 days post inoculation for banana and tomato plants in different culture systems with differential distribution of bacterial cells along tomato and banana roots. FISH and CARD–FISH methodologies were both successful in detecting B. ...
Source: Microbiological Research - September 13, 2018 Category: Infectious Diseases Source Type: research

The exopolysaccharide gene cluster pea is transcriptionally controlled by RpoS and repressed by AmrZ in Pseudomonas putida KT2440
In this study, we identified that the gene cluster pea was transcribed from 25 bp upstream of the operon and the stationary phase alternative sigma factor RpoS regulated the transcription of pea. When RpoS was absent, another sigma factor, likely the housekeeping sigma factor RpoD, could also mediate pea transcription but at a low level. The function of Pea polysaccharide was further confirmed to be necessary for full production of biofilm, formation of pellicle and c-di-GMP-dependent wrinkly colony morphology. Additionally, evidences were provided to demonstrate that the transcriptional regulator AmrZ was a negative regul...
Source: Microbiological Research - September 11, 2018 Category: Infectious Diseases Source Type: research

Application of laser microdissection to study phytoplasma site-specific gene expression in the model plant Arabidopsis thaliana
In this study, a procedure was established to infect the model plant Arabidopsis thaliana with the phytoplasma Flavescence dorée, a serious threat to European viticulture. Rates of phytoplasma infective insects and transmission efficiency to A. thaliana as well as pathogen loads were measured in different tissues of infected A. thaliana plants, and modification of phloem cell ultrastructure was observed in infected plant tissues at microscopic level. Moreover, a protocol for the application of laser microdissection to analyze plant and phytoplasma gene expression profiles in the specific colonized tissue was designe...
Source: Microbiological Research - September 11, 2018 Category: Infectious Diseases Source Type: research

Blue light exposure and nutrient conditions influence the expression of genes involved in simultaneous hyphal knot formation in Coprinopsis cinerea
Publication date: Available online 8 September 2018Source: Microbiological ResearchAuthor(s): Yuichi Sakamoto, Shiho Sato, Miyuki Ito, Yuki Ando, Kiyoshi Nakahori, Hajime MuraguchiAbstractLight and nutrients are crucial environmental factors influencing fungal sexual reproduction. Blue light induces simultaneous hyphal knot formation in Coprinopsis cinerea mycelia grown on low-glucose media but not in mycelia grown on high-glucose media. Many hyphal knots are visible in the arc near the edge of the colony one day after 15 min of blue light stimulation. These findings collectively suggest that blue light accelerates hypha...
Source: Microbiological Research - September 9, 2018 Category: Infectious Diseases Source Type: research

Isolation, characterization, and selection of heavy metal-resistant and plant growth-promoting endophytic bacteria from root nodules of Robinia pseudoacacia in a Pb/Zn mining area
In this study, we isolated and characterized 82 endophytic bacteria from the root nodules of black locust (Robinia pseudoacacia) grown in a Pb-Zn mining area. There were 80 isolates showing resistance to four HMs, 0.01–18.0 mM/L for Cd, 0.2–40.0 mM/L for Zn, 0.3–2.2 mM/L for Pb, and 0.2–1.4 mM/L for Cu. Indole-3-acetic acid production, siderophore production, and 1-aminocyclopropane-1-carboxylate deaminase activity were detected in 43, 50, and 17 isolates, respectively. Two symbiotic isolates selected with the highest potential for HM resistance and PGP traits, designated...
Source: Microbiological Research - September 7, 2018 Category: Infectious Diseases Source Type: research

Prospects of endophytic fungal entomopathogens as biocontrol and plant growth promoting agents: an insight on how artificial inoculation methods affect endophytic colonization of host plants
Publication date: Available online 31 August 2018Source: Microbiological ResearchAuthor(s): Bamisope Steve Bamisile, Chandra Kanta Dash, Komivi Senyo Akutse, Ravindran Keppanan, Oluwatoyin Grace Afolabi, Mubasher Hussain, Muhammad Qasim, Liande WangAbstractEntomopathogenic fungi (EPF) can be established as endophytes in the host plants to offer a long-term preventive measure for pests and diseases. This practice serves as a better alternative to the common practice of periodic direct application of EPF on plants or the target pests as a short-term defense strategy against pests and diseases. These fungal endophytes, aside ...
Source: Microbiological Research - September 1, 2018 Category: Infectious Diseases Source Type: research

Genome-wide transcriptome response of Streptomyces tsukubaensis to N-acetylglucosamine: effect on tacrolimus biosynthesis
We report here the negative effect of GlcNAc on differentiation and tacrolimus (FK506) production by Streptomyces tsukubaensis NRRL 18488. Using microarrays technology, we found that GlcNAc represses the transcription of fkbN, encoding the main transcriptional activator of the tacrolimus biosynthetic cluster, and of ppt1, encoding a phosphopantheteinyltransferase involved in tacrolimus biosynthesis. On the contrary, GlcNAc stimulated transcription of genes related to amino acid and nucleotide biosynthesis, DNA replication, RNA translation, glycolysis and pyruvate metabolism. The results obtained support those previously re...
Source: Microbiological Research - September 1, 2018 Category: Infectious Diseases Source Type: research

Biological control of the soft rot bacterium Pectobacterium carotovorum by Bacillus amyloliquefaciens strain Ar10 producing glycolipid-like compounds
Publication date: Available online 31 August 2018Source: Microbiological ResearchAuthor(s): Sana Azaiez, Imen Ben Slimene, Ines Karkouch, Rym Essid, Selim Jallouli, Naceur Djebali, Salem Elkahoui, Ferid Limam, Olfa TabbeneAbstractFour hundred and fifty bacteria were evaluated for antagonistic activity against bacterial soft rot of potato caused by Pectobacterium carotovorum sp strain II16. A strain Ar10 exhibiting potent antagonist activity has been identified as Bacillus amyloliquefaciens on the basis of biochemical and molecular characterization. Cell free supernatant showed a broad spectrum of antibacterial activity aga...
Source: Microbiological Research - September 1, 2018 Category: Infectious Diseases Source Type: research

Profiles of quorum sensing (QS)-related sequences in phycospheric microorganisms during a marine dinoflagellate bloom, as determined by a metagenomic approach
Publication date: Available online 30 August 2018Source: Microbiological ResearchAuthor(s): Xinqing Huang, Jianming Zhu, Zhonghua Cai, Yongmin Lao, Hui Jin, Ke Yu, Boya Zhang, Jin ZhouAbstractThe complicated relationships among environmental microorganisms are regulated by quorum sensing (QS). Understanding QS-based signals could shed light on the interactions between microbial communities in certain environments. Although QS characteristics have been widely discussed, few studies have been conducted on the role of QS in phycospheric microorganisms. Here, we used metagenomics to examine the profile of AI-1 (AinS, HdtS, Lux...
Source: Microbiological Research - August 31, 2018 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): (Source: Microbiological Research)
Source: Microbiological Research - August 30, 2018 Category: Infectious Diseases Source Type: research

Antifungal activity of spider venom-derived peptide lycosin-I against Candida tropicalis
Publication date: Available online 27 August 2018Source: Microbiological ResearchAuthor(s): Li Tan, Le Bai, Ling Wang, Lagu He, Guangdi Li, Wenhan Du, Ting Shen, Zheyi Xiang, Jiali Wu, Zhonghua Liu, Min HuAbstractCandida species are a major cause of human mucosal and deep tissue fungal infections, but few antifungal treatments are available. Here, we showed that lycosin-I, a peptide isolated from venom of the spider Lycosa singoriensis, acted as a potent antifungal inhibitor against Candida species. The MIC50 values of lycosin-I reached 8 µg/mL to treat fluconazole-susceptible and fluconazole-resistant C. tropicali...
Source: Microbiological Research - August 28, 2018 Category: Infectious Diseases Source Type: research

Transcriptional regulation of galF by RcsAB affects capsular polysaccharide formation in Klebsiella pneumoniae NTUH-K2044
In conclusion, RcsAB could regulate the transcription of the galF gene positively by binding to the galF promoter DNA directly, and then affects the CPS formation of K. pneumonia. (Source: Microbiological Research)
Source: Microbiological Research - August 26, 2018 Category: Infectious Diseases Source Type: research