Codon-usage frequency mediated SNPs selection in lasR gene of cystic fibrosis Pseudomonas aeruginosa isolates
Publication date: Available online 9 May 2019Source: Microbiological ResearchAuthor(s): Huifang Qiu, Yuanhao Li, Weijun DaiAbstractPseudomonas aeruginosa is an opportunistic pathogen with high clinical relevance for hospital infections of patients. Accumulating DNA sequencing results of clinical P. aeruginosa isolates have revealed frequent mutations in lasR gene, which encodes the highest arches component of quorum-sensing system (QS). We analyzed the sequencing data of lasR gene from a large collection of cystic fibrosis (CF) P. aeruginosa isolates. Our systematical analyses revealed that single nucleotide polymorphisms ...
Source: Microbiological Research - May 11, 2019 Category: Infectious Diseases Source Type: research

Anti-quorum sensing and anti-biofilm activity of 5-Hydroxymethylfurfural against Pseudomonas aeruginosa PAO1: insights from in vitro, in vivo and in silico studies
Publication date: Available online 7 May 2019Source: Microbiological ResearchAuthor(s): Jobina Rajkumari, Subhomoi Borkotoky, Dhanasekhar Reddy, Saswat Kumar Mohanty, Ranjith Kumavath, Ayaluru Murali, Kitlangki Suchiang, Siddhardha BusiAbstractPseudomonas aeruginosa is one of the most common pathogens associated with nosocomial infections and a great concern to immunocompromised individuals especially in the cases of cystic fibrosis, AIDS and burn wounds. The pathogenicity of P. aeruginosa is largely directed by the quorum sensing (QS) system. Hence, QS may be considered an important therapeutic target to combat P. aerugin...
Source: Microbiological Research - May 9, 2019 Category: Infectious Diseases Source Type: research

RNA interference and CRISPR: Promising approaches to better understand and control citrus pathogens
Publication date: Available online 27 April 2019Source: Microbiological ResearchAuthor(s): Eduardo Henrique Goulin, Diogo Manzano Galdeano, Laís Moreira Granato, Emilyn Emy Matsumura, Ronaldo José Durigan Dalio, Marcos Antonio MachadoAbstractCitrus crops have great economic importance worldwide. However, citrus production faces many diseases caused by different pathogens, such as bacteria, oomycetes, fungi and viruses. To overcome important plant diseases in general, new technologies have been developed and applied to crop protection, including RNA interference (RNAi) and clustered regularly interspaced short...
Source: Microbiological Research - April 29, 2019 Category: Infectious Diseases Source Type: research

A multifaceted rhizobacterium Paenibacillus lentimorbus alleviates nutrient deficiency-induced stress in Cicer arietinum L
Publication date: Available online 25 April 2019Source: Microbiological ResearchAuthor(s): Nikita Bisht, Shalini Tiwari, Poonam C. Singh, Abhishek Niranjan, Puneet Singh ChauhanABSTRACTNutrient deficiency in soil is one of the limiting factors responsible for stunted growth and poor flowering/fruiting of crops which result in decline in overall agricultural productivity. However, one important strategy to overcome the problem of nutrient deficiency and to avoid use of chemical fertilizers is the use of plant growth promoting rhizobacteria (PGPR). Paenibacillus lentimorbus NRRL B-30488 (hereafter B-30488), an efficient PGPR...
Source: Microbiological Research - April 26, 2019 Category: Infectious Diseases Source Type: research

Regulation of antimonite oxidation and resistance by the phosphate regulator PhoB in Agrobacterium tumefaciens GW4
In this study, comprehensive analyses were performed and the results showed that (1) Genomic analysis revealed two phoB (named as phoB1 and phoB2) and one phoR gene in strain GW4; (2) Reporter gene assay showed that both phoB1 and phoB2 were induced in low phosphate condition (50 μM), but only phoB2 was induced by Sb(III); (3) Genes knock-out/complementation, Sb(III) oxidation and Sb(III) resistance tests showed that deletion of phoB2 significantly inhibited the expression of anoA and decreased bacterial Sb(III) oxidation efficiency and Sb(III) resistant. In contrast, deletion of phoB1 did not obviously affect an...
Source: Microbiological Research - April 26, 2019 Category: Infectious Diseases Source Type: research

Heterobasidion-growth inhibiting Bacillus subtilis A18 exhibits medium- and age-dependent production of lipopeptides
Publication date: Available online 22 April 2019Source: Microbiological ResearchAuthor(s): Muhammad Azeem, Marina Barba-Aliaga, Anna Karin Borg-Karlson, Olle Terenius, Anders Broberg, Gunaratna Kuttuva RajaraoAbstractHeterobasidion annosum s.s. and H. parviporum are severe pathogens of conifers causing butt rot and root rot thus reducing the economic value of timber. Here, the antifungal activity of Bacillus subtilis isolate A18 against these two Heterobasidion species was investigated. Five different culture media with different culture age were investigated to study the effect of substrate composition and culture age for...
Source: Microbiological Research - April 23, 2019 Category: Infectious Diseases Source Type: research

Beneficial effects of inoculation of growth-promoting bacteria in strawberry
The objective of this study was to verify the potential of plant growth promotion of 25 wild isolates from the Agricultural Microbiology Culture Collection of the Federal University of Lavras (CCMA-UFLA) through the evaluation of the biological nitrogen-fixation capacity and the production of IAA. In addition, the growth of three selected strains inoculated on roots of strawberry seedlings in greenhouse conditions was evaluated. The experiment was conducted in a completely randomized design (CRD), with an 8 × 2 factorial schemes involving eight combinations of bacteria: alone, in pairs and threes, plus ...
Source: Microbiological Research - April 21, 2019 Category: Infectious Diseases Source Type: research

Quorum-dependent expression of rsmX and rsmY, small non-coding RNAs, in Pseudomonas syringae
Publication date: Available online 12 April 2019Source: Microbiological ResearchAuthor(s): Yukiko Nakatsu, Hidenori Matsui, Mikihiro Yamamoto, Yoshiteru Noutoshi, Kazuhiro Toyoda, Yuki IchinoseAbstractPseudomonas syringae pathovars are known to produce N-acyl-homoserine lactones (AHL) as quorum-sensing molecules. However, many isolates, including P. syringae pv. tomato DC3000 (PtoDC3000), do not produce them. In P. syringae, psyI, which encodes an AHL synthase, and psyR, which encodes the transcription factor PsyR required for activation of psyI, are convergently transcribed. In P. amygdali pv. tabaci 6605 (Pta6605), there...
Source: Microbiological Research - April 14, 2019 Category: Infectious Diseases Source Type: research

Role of ClpX and ClpP in Streptococcus suis serotype 2 stress tolerance and virulence
In this study, a virulent S. suis serotype 2 strain, ZY05719, was employed to construct clpX and clpP deletion mutants (ΔclpX and ΔclpP, respectively) and their complementation strains. Both ΔclpX and ΔclpP displayed significantly reduced adaptability compared with the wild-type strain, evident through several altered phenotypes: formation of long cell chains, tendency to aggregate in culture, and reduced growth under acidic pH and H2O2-induced oxidative stress. ClpP and ClpX were required for the optimal growth during heat and cold stress, respectively. An in vitro experiment on RAW264.7 macrophage...
Source: Microbiological Research - April 14, 2019 Category: Infectious Diseases Source Type: research

Understanding the multifaceted roles of the phosphoenolpyruvate: phosphotransferase system in regulation of Salmonella virulence using a mutant defective in ptsI and crr expression
In this study, the role of PTS in Salmonella virulence regulation was explored. To decipher the regulatory network coordinated by the PTS during Salmonella infection, a transcriptomic approach was applied to a transposon insertion mutant with defective expression of ptsI and crr, which encode enzyme I and enzyme IIAGlc of the PTS, respectively. There were 114 differentially expressed genes (DEGs) exhibiting two-fold or higher expression changes in the transposon mutant strain, with 13 up-regulated genes versus 101 down-regulated genes. One-third of the DEGs were associated with energy production and carbohydrate/amino acid...
Source: Microbiological Research - April 14, 2019 Category: Infectious Diseases Source Type: research

Co-regulation of CodY and (p)ppGpp synthetases on morphology and pathogenesis of Streptococcus suis
This study extends our understanding of the relationship between the (p)ppGpp-mediated stringent response and the regulation of CodY in S. suis. (Source: Microbiological Research)
Source: Microbiological Research - April 9, 2019 Category: Infectious Diseases Source Type: research

Dissection of ToxR-dependent and ToxR-independent stress-regulated pathways in Vibrio parahaemolyticus
In this study, we demonstrated that ToxR is essential for V. parahaemolyticus to tolerate acid stress by constructing a ToxR deletion mutant (ΔtoxR) and its complemented strain (toxR+). Quantitative PCR showed that the expression of toxR was up regulated under acid stress condition. RNA-seq analysis showed that ompU encoding one of outer membrane proteins was dramatically down regulated in ΔtoxR. Furthermore, the mutation of ompU also led to a significant reduction in tolerating acid stress indicating that ToxR mediated acid stress through regulating ompU expression. RNA-seq results further confirmed that acid ...
Source: Microbiological Research - April 7, 2019 Category: Infectious Diseases Source Type: research

Molecular and biological characterization of phytoplasmas from coconut palms affected by the lethal yellowing disease in Africa
This study reports the first biochemical characterization of two of these phytoplasma isolates. Moreover, a disc-diffusion antibiotic susceptibility assay revealed that these bacteria exhibit tobramycin susceptibility and cephalexin hydrate and rifampicin resistance. Urea and arginine hydrolysis, and glucose fermentation tests that were performed on colonies of phytoplasmas and Acholeplasma laidlawii indicated that both phytoplasmas tested were negative for urea and positive for glucose and arginine, whereas A. laidlawii was positive for glucose and negative for urea and arginine. The growth of coconut phytoplasma in both ...
Source: Microbiological Research - April 3, 2019 Category: Infectious Diseases Source Type: research

Characterisation of ParB encoded on multipartite genome in Deinococcus radiodurans and their roles in radioresistance
Publication date: Available online 1 April 2019Source: Microbiological ResearchAuthor(s): Ganesh K Maurya, Swathi Kota, Hari S. MisraAbstractThe Deinococcus radiodurans multipartite genome consists of 2 chromosomes and 2 plasmids Its genome encodes 4 ParA and 4 ParB proteins on different replicons. Multiple sequence alignments of ParBs encoded on these genome elements showed that ParB of primary chromosome (ParB1) is close to chromosomal type ParB and is found to be different from ParBs encoded on chromosome II (ParB2) and megaplasmid (ParB3) elements. We observed that ParB1, ParB2 and ParB3 exist as dimer in solution and ...
Source: Microbiological Research - April 3, 2019 Category: Infectious Diseases Source Type: research

Bacterial seed endophyte community of annual plants modulated by plant photosynthetic pathways
Publication date: Available online 29 March 2019Source: Microbiological ResearchAuthor(s): Ruben Girsowicz, Itumeleng Moroenyane, Yosef SteinbergerAbstractClimate change is predicted to have adverse impacts on terrestrial ecosystems and uncertainties exist on how these systems will respond. Terrestrial plant ecosystems can be divided by how they fix atmospheric carbon- C3, C4 and CAM photosynthesis pathways. However, as for now, no clear answers could be given regarding the future global repartition of the C3, C4 and CAM plants. As seeds are the reproductive and dispersal unit of the plants and endophytes play a central ro...
Source: Microbiological Research - March 31, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: May 2019Source: Microbiological Research, Volume 222Author(s): (Source: Microbiological Research)
Source: Microbiological Research - March 28, 2019 Category: Infectious Diseases Source Type: research

Bacterial endophytes of mangrove propagules elicit early establishment of the natural host and promote growth of cereal crops under salt stress
Publication date: Available online 26 March 2019Source: Microbiological ResearchAuthor(s): Riccardo Soldan, Francesca Mapelli, Elena Crotti, Sylvia Schnell, Daniele Daffonchio, Ramona Marasco, Marco Fusi, Sara Borin, Massimiliano CardinaleAbstractMangroves, dominating tropical intertidal zones and estuaries, are among the most salt tolerant plants, and propagate through reproductive units called propagules. Similarly to other plants’ seeds, propagules may harbor beneficial bacteria. Our hypothesis was that mangroves, being able to grow into seawater, should harbor bacteria able to interact with the host and to exert ...
Source: Microbiological Research - March 27, 2019 Category: Infectious Diseases Source Type: research

Highly efficient single base editing in Aspergillus niger with CRISPR/Cas9 cytidine deaminase fusion
Publication date: Available online 23 March 2019Source: Microbiological ResearchAuthor(s): Lianggang Huang, Hongzhi Dong, Junwei Zheng, Bin Wang, Li PanAbstractClassic genome editing tools including ZFN, TALEN, and CRISPR/Cas9 rely on DNA double-strand breaks for genome editing. To prevent the potential hazard caused by double-strand breaks (DSBs), a series of single base editing tools that convert cytidine (C) to thymine (T) without DSBs have been developed extensively in multiple species. Herein, we report for the first time that C was converted to T with a high frequency in the filamentous fungi Aspergillus niger by fus...
Source: Microbiological Research - March 25, 2019 Category: Infectious Diseases Source Type: research

Characterization of the cellulase-secretome produced by the Antarctic bacterium Flavobacterium sp. AUG42
Publication date: Available online 23 March 2019Source: Microbiological ResearchAuthor(s): Lorena M. Herrera, Victoria Braña, Laura Franco Fraguas, Susana Castro-SowinskiAbstractFlavobacterium sp. AUG42 is a cellulase-producing bacterium isolated from the Antarctic oligochaete Grania sp. (Annelida). In this work, we report that AUG42 produces a glycoside hydrolase cocktail with CMCase, PASCase and cellobiase activities (optimum pHs and temperatures ranging from 5.5 to 6.5 and 40 to 50 °C, respectively). The time-course analyses of the bacterial growth and cellulase production showed that the cocktail has ...
Source: Microbiological Research - March 25, 2019 Category: Infectious Diseases Source Type: research

Glucose dehydrogenase gene containing phosphobacteria for biofortification of Phosphorus with growth promotion of rice
Publication date: Available online 22 March 2019Source: Microbiological ResearchAuthor(s): Maria Rasul, Sumera Yasmin, M. Suleman, Ahmad Zaheer, Thomas Reitz, Mika T. Tarkka, Ejazul Islam, M. Sajjad MirzaAbstractPhosphorus (P) is an essential plant nutrient, but often limited in soils for plant uptake. A major economic constraint in the rice production is excessive use of chemical fertilizers to meet the P requirement. Bioaugmentation of phosphate solubilizing rhizobacteria (PSB) can be used as promising alternative. In the present study 11 mineral PSB were isolated from Basmati rice growing areas of Pakistan. In broth med...
Source: Microbiological Research - March 23, 2019 Category: Infectious Diseases Source Type: research

Prediction of in situ metabolism of photobacteria in modified atmosphere packaged poultry meat using metatranscriptomic data
Publication date: Available online 8 March 2019Source: Microbiological ResearchAuthor(s): Linda Höll, Maik Hilgarth, Andreas J. Geissler, Jürgen Behr, Rudi F. VogelAbstractModified atmosphere packaging (MAP) is widely used in food industry to extend the microbiological shelf life of meat. Common CO2-containing gas atmospheres for poultry meat packaging are either nearly O2-free or high O2 MAPs. In this work, we compared spoilage microbiota of skinless chicken breast in CO2/O2 (30/70%) and CO2/N2 (30/70%) MAP, which are culturable with conventional methods and identified isolates by MALDI-TOF MS. These data were c...
Source: Microbiological Research - March 9, 2019 Category: Infectious Diseases Source Type: research

Molecular mechanisms of Vibrio parahaemolyticus pathogenesis
This article comprehensively reviewed the molecular mechanisms of eight types of virulence factors: hemolysin, type III secretion system, type VI secretion system, adhesion factor, iron uptake system, lipopolysaccharide, protease and outer membrane proteins. This review comprehensively summarized our current understanding of the virulence factors in V. parahaemolyticus, which are potentially new targets for the development of therapeutic and preventive strategies. (Source: Microbiological Research)
Source: Microbiological Research - March 9, 2019 Category: Infectious Diseases Source Type: research

Shotgun proteomics provides an insight into pathogenesis-related proteins using anamorphic stage of the biotroph, Erysiphe pisi pathogen of garden pea
In this study, the proteome of the anamorphic stage of E. pisi pathogen has been elucidated through the nano LC–MS/MS approach. A total of 328 distinct proteins were detected from Erysiphe isolates infecting the susceptible pea cultivar, Arkel. The proteome is available via ProteomeXchange with identifier PXD010238. The functional classification of protein accessions based on Gene Ontology revealed proteins related to signal transduction, secondary metabolite formation and stress which might be involved in virulence and pathogenesis. The functional validation carried through differential expression of genes encoding ...
Source: Microbiological Research - March 6, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: April 2019Source: Microbiological Research, Volume 221Author(s): (Source: Microbiological Research)
Source: Microbiological Research - February 28, 2019 Category: Infectious Diseases Source Type: research

Retraction notice to "MarTrack: A versatile toolbox of mariner transposon derivatives used for functional genetic analysis of bacterial genomes" [Microbiological Research 219 (2019) 84–93]
Publication date: April 2019Source: Microbiological Research, Volume 221Author(s): Lifan Wei, Haoxian Qiao, Bing Liu, Kaiyu Yina, Qin Liua, Yuanxing Zhang, Yue Ma, Qiyao Wang (Source: Microbiological Research)
Source: Microbiological Research - February 28, 2019 Category: Infectious Diseases Source Type: research

Retraction notice to "Genome sequence and comparative genomics of Rhizobium sp. Td3, a novel plant growth promoting phosphate solubilizing Cajanus cajan symbiont" [Microbiological Research 218 (2019) 32–40]
Publication date: April 2019Source: Microbiological Research, Volume 221Author(s): Bhagya Iyer, Shalini Rajkumar (Source: Microbiological Research)
Source: Microbiological Research - February 28, 2019 Category: Infectious Diseases Source Type: research

Search for bacterial α1,2-fucosyltransferases for whole-cell biosynthesis of 2′-fucosyllactose in recombinant Escherichia coli
In this study, 10 α1,2-FT genes from bacteria of biosafety level one were identified, and the main features of the deduced amino acid sequences were characterized. Four codon-optimized α1,2-FT genes were synthesized and introduced into Escherichia coli ΔL M15 strain containing the plasmid pBCGW encoding guanosine 5′-diphosphate-L-fucose biosynthetic enzymes. Among the four genes, 2′-FL was produced only by the α1,2-FT from Thermosynechococcus elongatus (Te2FT). Bifidobacterium thermacidophilum α1,2-FT (Bt2FT) showed high expression but was not active in E. coli ΔL M15. The ot...
Source: Microbiological Research - February 26, 2019 Category: Infectious Diseases Source Type: research

The Agrobacterium tumefaciens atu3184 gene, a member of the COG0523 family of GTPases, is regulated by the transcriptional repressor Zur
Publication date: Available online 23 February 2019Source: Microbiological ResearchAuthor(s): Sasimaporn Khemthong, Puttamas Nuonming, Sumontha Nookabkaewb, Rojana Sukchawalit, Skorn MongkolsukAbstractAnalysis of the Agrobacterium tumefaciens C58 genome revealed a potential Zur (zinc uptake regulator) binding site (5′-GATATGTTATTACATTAC-3′, the underlined letters are the center of symmetry of the inverted palindrome) located in the upstream region of atu3184, whose gene product is a member of the COG0523 subfamily of G3E GTPases. The specific interaction of the Zur protein with the 18-bp inverted repeat operato...
Source: Microbiological Research - February 25, 2019 Category: Infectious Diseases Source Type: research

Multicopper oxidases: biocatalysts in microbial pathogenesis and stress management
Publication date: Available online 16 February 2019Source: Microbiological ResearchAuthor(s): Kavleen Kaur, Aarjoo Sharma, Neena Capalash, Prince SharmaAbstractThe acquisition of metal ions such as iron, copper and manganese is essential for the survival of microorganisms as these are constituents of metalloproteins including enzymes, storage proteins, structural elements, transcription factors and antimicrobial factors in various biological processes. However, excess of these metal ions is associated with significant toxicity due to spontaneous redox cycling of ions and obstruction of normal metabolic pathways. To overcom...
Source: Microbiological Research - February 17, 2019 Category: Infectious Diseases Source Type: research

Shotgun proteomics provides an insight into pathogenesis related proteins using anamorphic stage of the biotroph, Erysiphe pisi pathogen of garden pea
In this study, the proteome of the anamorphic stage of E. pisi pathogen has been elucidated through the nano LC-MS/MS approach. A total of 328 distinct proteins were detected from Erysiphe isolates infecting the susceptible pea cultivar, Arkel. The proteome is available via ProteomeXchange with identifier PXD010238. The functional classification of protein accessions based on Gene Ontology revealed proteins related to signal transduction, secondary metabolite formation and stress which might be involved in virulence and pathogenesis. The functional validation carried through differential expression of genes encoding G-prot...
Source: Microbiological Research - February 13, 2019 Category: Infectious Diseases Source Type: research

Enhanced Nitrogen and Phosphorus Activation with an Optimized Bacterial Community by Endophytic Fungus Phomopsis liquidambari in Paddy Soil
Publication date: Available online 11 February 2019Source: Microbiological ResearchAuthor(s): Meng-Jun Tang, Qiang Zhu, Feng-Min Zhang, Wei Zhang, Jie Yuan, Kai Sun, Fang-Ji Xu, Chuan-Chao DaiAbstractThe endophytic fungus Phomopsis liquidambari play a key role in habitat adaptation of rice (Oryza sativa L.) with potential multiple beneficial. However, our previous published work on this subject remains incomplete. Here, we performed a soil nutrient (nitrogen and phosphorus) transformation with related functional genes and elucidated how rhizosphere microbiota vary their response to P. liquidambari interaction throughout th...
Source: Microbiological Research - February 12, 2019 Category: Infectious Diseases Source Type: research

An alternative biocontrol agent of soil-borne phytopathogens: a new antifungal compound produced by a plant growth promoting bacterium isolated from North Algeria
Publication date: Available online 10 February 2019Source: Microbiological ResearchAuthor(s): Bruna Agrillo, Sara Mirino, Rosarita Tatè, Lorena Gratino, Marta Gogliettino, Ennio Cocca, Nassira Tabli, Elhafid Nabti, Gianna PalmieriAbstractBacteria isolated from different environments can be exploited for biocontrol purposes by the identification of the molecules involved in the antifungal activity. The present study was aimed at investigating antifungal protein compounds purified from a previously identified plant growth promoting bacterium, Pseudomonas protegens N isolated from agricultural land in northern Algeria....
Source: Microbiological Research - February 10, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: March 2019Source: Microbiological Research, Volume 220Author(s): (Source: Microbiological Research)
Source: Microbiological Research - February 9, 2019 Category: Infectious Diseases Source Type: research

Plant Beneficial Endophytic Bacteria: Mechanisms, Diversity, Host Range and Genetic Determinants
Publication date: Available online 4 February 2019Source: Microbiological ResearchAuthor(s): Imran Afzal, Zabta Khan Shinwari, Shomaila Sikandar, Shaheen ShahzadAbstractEndophytic bacteria are the plant beneficial bacteria that thrive inside plants and can improve plant growth under normal and challenging conditions. They can benefit host plants directly by improving plant nutrient uptake and by modulating growth and stress related phytohormones. Indirectly, endophytic bacteria can improve plant health by targeting pests and pathogens with antibiotics, hydrolytic enzymes, nutrient limitation, and by priming plant defenses....
Source: Microbiological Research - February 5, 2019 Category: Infectious Diseases Source Type: research

Communication mechanisms in extremophiles: Exploring their existence and industrial applications
Publication date: Available online 18 January 2019Source: Microbiological ResearchAuthor(s): Amandeep Kaur, Neena Capalash, Prince SharmaAbstractQuorum sensing plays important roles in the regulation of physiological and virulence processes in most bacteria, but its role in extremophiles is largely unknown. Comparative genomic, phylogenic, structural and signaling pathways analyses and deletion mutant studies have suggested the presence of three major quorum sensing systems (AI-1, Peptide based and AI-2) in extremophiles. Autoinducer-1(AI-1) system was found to be most prevalent (except in thermophiles where it is autoindu...
Source: Microbiological Research - January 20, 2019 Category: Infectious Diseases Source Type: research

Nodule-associated microbiome diversity in wild populations of Sulla coronaria reveals clues on the relative importance of culturable rhizobial symbionts and co-infecting endophytes
Publication date: Available online 18 January 2019Source: Microbiological ResearchAuthor(s): Rosella Muresu, Andrea Porceddu, Leonardo Sulas, Andrea SquartiniAbstractThe culturable bacteria from root nodules of Sulla coronaria growing in spontaneous conditions in Sardinia were characterized. This plant’s peculiarity is to represent a legume still found in both wild and cropped statuses. We tested whether culturable bacteria would differ from those commonly isolated from its field-cropped varieties, to date exclusively represented by Rhizobium sullae. 63 isolates from 60 surface-sterilized nodules were analyzed by ARD...
Source: Microbiological Research - January 20, 2019 Category: Infectious Diseases Source Type: research

Muscodor brasiliensis sp. nov. produces volatile organic compounds with activity against Penicillium digitatum
Publication date: Available online 18 January 2019Source: Microbiological ResearchAuthor(s): Lorena C. Pena, Gustavo H. Jungklaus, Daiani C. Savi, Lisandra Ferreira-Maba, André Servienski, Beatriz H.L.N.S. Maia, Vinicius Annies, Lygia V. Galli-Terasawa, Chirlei Glienke, Vanessa KavaAbstractEndophytic fungi belonging to Muscodor genus are considered as promising alternatives to be used in biological control due to the production of volatile organic compounds (VOCs). The strains LGMF1255 and LGMF1256 were isolated from the medicinal plant Schinus terebinthifolius and, by morphological data and phylogenetic analysis, i...
Source: Microbiological Research - January 20, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: February 2019Source: Microbiological Research, Volume 219Author(s): (Source: Microbiological Research)
Source: Microbiological Research - January 17, 2019 Category: Infectious Diseases Source Type: research

Comparative genomics of Lactobacillus sakei supports the development of starter strain combinations
Publication date: Available online 14 January 2019Source: Microbiological ResearchAuthor(s): Lara Eisenbach, Andreas J. Geissler, Matthias A. Ehrmann, Rudi F. VogelAbstractStrains of Lactobacillus sakei can be isolated from a variety of sources including meat, fermented sausages, sake, sourdough, sauerkraut or kimchi. Selected strains are widely used as starter cultures for sausage fermentation. Recently we have demonstrated that control about the lactic microbiota in fermenting sausages is achieved rather by pairs or strain sets than by single strains. In this work we characterized the pan genome of L. sakei to enable exp...
Source: Microbiological Research - January 15, 2019 Category: Infectious Diseases Source Type: research

Differential involvement of glycans in the binding of Staphylococcus epidermidis and Corynebacterium spp. to human sweat
Publication date: Available online 30 December 2018Source: Microbiological ResearchAuthor(s): Chi-Hung Lin, Robyn A Peterson, Audrey Gueniche, Ségolène Adam de Beaumais, Virginie Hourblin, Lionel Breton, Maria Dalko, Nicolle H. PackerAbstractSweat is a secretory fluid that can be a source of unpleasant body odour due to interaction of resident bacteria with sweat components. Identification of glycoproteins in sweat suggests that protein-conjugated glycans may act as binding epitopes for bacteria, as found in other secretory fluids such as human milk, tears and saliva which help to protect epithelial surfaces ...
Source: Microbiological Research - December 30, 2018 Category: Infectious Diseases Source Type: research

Differential gene expression in two grapevine cultivars recovered from “flavescence dorée”
Publication date: Available online 28 December 2018Source: Microbiological ResearchAuthor(s): Davide Pacifico, Paolo Margaria, Luciana Galetto, Mauro Legovich, Simona Abbà, Flavio Veratti, Cristina Marzachì, Sabrina PalmanoAbstractThe biological bases of recovery of two grapevine cultivars, Nebbiolo and Barbera, showing different susceptibility and recovery ability to “flavescence dorée” (FD) phytoplasma infection were investigated. The expression over one vegetative season, in FD-recovered and healthy grapevines, of 18 genes involved in defence, hydrogen peroxide and hormone production was...
Source: Microbiological Research - December 29, 2018 Category: Infectious Diseases Source Type: research

Sestrin-like protein from Dictyostelium discoideum is involved in autophagy under starvation stress
Publication date: Available online 28 December 2018Source: Microbiological ResearchAuthor(s): S Rafia, S SaranAbstractSestrins are highly conserved; stress inducible proteins that help maintain metabolic homeostasis and protect cells under stress conditions. They are up-regulated during stress and influence AMPK and mTOR pathways. Our objective was to find the role of Sestrin protein from Dictyostelium discoideum (Dd), a lower eukaryote where starvation stress initiates multicellular development. The single DdSesn-like gene was expressed and its endogenous functions were characterized. Both, the knockout and constitutively...
Source: Microbiological Research - December 29, 2018 Category: Infectious Diseases Source Type: research

Reactive green dye remediation by Alternanthera philoxeroides in association with plant growth promoting Klebsiella sp. VITAJ23; A pot culture study
Publication date: Available online 21 December 2018Source: Microbiological ResearchAuthor(s): Astha Sinha, Sajitha Lulu S, Vino S, W.Jabez OsborneAbstractContamination of soil by textile effluent is a major threat found worldwide. These pollutants have a diverse negative effects on the ecosystem, therefore restoration through a cost biological strategy is the need of the hour. The aim of the current study was to enhance the decolourization of reactive green dye (RGD) by phytoremediation coupled with augmentation of effective bacteria to the rhizosphere. The isolate Klebsiella sp. VITAJ23 was isolated from textile polluted ...
Source: Microbiological Research - December 22, 2018 Category: Infectious Diseases Source Type: research

Specific regions of the SulA protein recognized and degraded by the ATP-dependent ClpYQ (HslUV) protease in Escherichia coli
In this study, using yeast two-hybrid and in vivo degradation analyses, we first demonstrated that the C-terminal internal hydrophobic region (139th~ 149th aa) of SulA is necessary for binding and degradation by ClpYQ. A conserved region, GFIMRP, between 142th and 147th residues of SulA, were identified among various Gram-negative bacteria. By using MBP-SulA(F143Y) (phenylalanine substituted with tyrosine) as a substrate, our results showed that this conserved residue of SulA is necessary for recognition and degradation by ClpYQ. Supporting these data, MBP-SulA(F143Y), MBP-SulA(F143 N) (phenylalanine substituted wit...
Source: Microbiological Research - December 18, 2018 Category: Infectious Diseases Source Type: research

The stringent response factor, RelA, positively regulates T6SS4 expression through the RovM/RovA pathway in Yersinia pseudotuberculosis
Publication date: Available online 6 December 2018Source: Microbiological ResearchAuthor(s): Xiaobing Yang, Yunhong Song, Qingyun Dai, Hongyun Zhang, Li Song, Zhuo Wang, Junfeng Pan, Yao WangAbstractThe type VI secretion system (T6SS) is a versatile molecular machinery widely distributed in Gram-negative bacteria. The activity of the T6SS is tightly regulated by various mechanisms, including quorum sensing (QS), iron concentration, and transcriptional regulators. Here we demonstrated that the stringent response regulator, RelA, contributes to bacterial resistance to multiple environmental stresses in Yersinia pseudotubercu...
Source: Microbiological Research - December 7, 2018 Category: Infectious Diseases Source Type: research

Control of Fusarium wilt of lisianthus by reassembling the microbial community in infested soil through reductive soil disinfestation
In this study, a pre-cultivation soil management strategy, reductive soil disinfestation (RSD), involving amendment by the use of labile organic substrates, such as reed straw, bagasse, and rice straw, and creating anaerobic soil conditions, was used to regulate the microbial community in a soil infested by Fusarium wilt of lisianthus and make it suitable for plant cultivation. The results showed that RSD significantly decreased F. oxysporum population by 97.1%–99.1% and the incidence of lisianthus wilt disease to 3.0%–14.3% compared with that of the untreated soil. The lowest disease incidence was found in the...
Source: Microbiological Research - December 7, 2018 Category: Infectious Diseases Source Type: research

Avocado rhizobacteria emit volatile organic compounds with antifungal activity against Fusarium solani, Fusarium sp. associated with Kuroshio shot hole borer, and Colletotrichum gloeosporioides
Publication date: Available online 24 November 2018Source: Microbiological ResearchAuthor(s): Edgar Guevara-Avendaño, Alix Adriana Bejarano-Bolívar, Ana-Luisa Kiel-Martínez, Mónica Ramírez-Vázquez, Alfonso Méndez-Bravo, Eneas Aguirre von Wobeser, Diana Sánchez-Rangel, José A. Guerrero-Analco, Akif Eskalen, Frédérique ReverchonAbstractRecent studies showed that bacterial volatile organic compounds (VOCs) play an important role in the suppression of phytopathogens. The ability of VOCs produced by avocado (Persea americana Mill.) rhizobacteria to sup...
Source: Microbiological Research - November 25, 2018 Category: Infectious Diseases Source Type: research

MarTrack: A versatile toolbox of mariner transposon derivatives used for functional genetic analysis of bacterial genomes
In this study, we combined the merits of the random mutagenesis of mariner transposon and common efficient reporter marker genes or regulatory elements, mcherry, gfp, luxAB, lacZ, sacBR, and PBAD and antibiotic resistance cassettes to construct a series of derivative transposon vectors, pMmch, pMKGR, pMCGR, pMXKGR, pMLKGR, pMSGR, and pMPR, based on the initial transposon pMar2xT7. The function and effectiveness of the modified transposons were verified by introducing them into E. piscicida EIB202. Based on the toolbox, a transposon insertion mutant library containing approximately 3.0 × 105 separated mu...
Source: Microbiological Research - November 24, 2018 Category: Infectious Diseases Source Type: research

Pseudomonas protegens MP12: A plant growth-promoting endophytic bacterium with broad-spectrum antifungal activity against grapevine phytopathogens
Publication date: Available online 23 November 2018Source: Microbiological ResearchAuthor(s): Marco Andreolli, Giacomo Zapparoli, Elisa Angelini, Gianluca Lucchetta, Silvia Lampis, Giovanni ValliniAbstractPseudomonas sp. MP12 was isolated from a soil sample collected in a typical warm-temperate deciduous forest near Brescia, northern Italy. Phylogenetic analysis identified the species as Pseudomonas protegens. We detected the genes phlD, pltB and prnC, which are required to synthesize the antifungal compounds 2,4-diacetylphloroglucinol (2,4-DAPG), pyoluteorin and pyrrolnitrin, respectively. P. protegens MP12 was also shown...
Source: Microbiological Research - November 24, 2018 Category: Infectious Diseases Source Type: research

Interactions between Pseudomonas spp. and their role in improving the red pepper plant growth under salinity stress
Publication date: Available online 23 November 2018Source: Microbiological ResearchAuthor(s): Sandipan Samaddar, Poulami Chatterjee, Aritra Roy Choudhury, Shamim Ahmed, Tongmin SaAbstractSolitary inoculation of bacteria has been studied widely for plant growth development and amelioration of salinity stress but co-inoculation of bacteria for salt stress amelioration in red pepper plants has been less studied till date. Here, we investigated the co-inoculation effect of Pseudomonas frederiksbergensis OB139 and Pseudomonas vancouverensis OB155 in red pepper plant growth characteristics, plant photosynthesis pigments, ethylen...
Source: Microbiological Research - November 24, 2018 Category: Infectious Diseases Source Type: research