Analysis of two Mexican Pectobacterium brasiliense strains reveals an inverted relationship between c-di-GMP levels with exopolysaccharide production and swarming motility
Publication date: Available online 19 February 2020Source: Microbiological ResearchAuthor(s): Delia A. Narváez-Barragán, Andrés de Sandozequi, Mabel Rodríguez, Karel Estrada, Omar E. Tovar-Herrera, Claudia Martínez-AnayaAbstractPectobacterium is a diverse genus of phytopathogenic species from soil and water that cause infection either to restricted or multiple plant hosts. Phylogenetic analysis and metabolic fingerprinting of large numbers of genomes have expanded classification of Pectobacterium members. Pectobacterium brasiliense sp. nov has been elevated to the species level having det...
Source: Microbiological Research - February 20, 2020 Category: Infectious Diseases Source Type: research

Bacteroidetes can be a rich source of novel lanthipeptides: the case study of Pedobacter lusitanus
Publication date: Available online 19 February 2020Source: Microbiological ResearchAuthor(s): Tânia Caetano, Wilfred van der Donk, Sónia MendoABSTRACTLanthipeptides are intriguing peptides known since 1928, the year of penicillin’s discovery. At that time, they were known as lantibiotics due to their (methyl)lanthionine amino acids and antibacterial activity. Their body of knowledge expanded tremendously over the last few years. Our analysis reveals that Bacteroidetes has a high state of clusters encoding the biosynthesis of class I lanthipeptides. We show that some strains of Pedobacter have a number of...
Source: Microbiological Research - February 19, 2020 Category: Infectious Diseases Source Type: research

Forest tree associated bacteria for potential biological control of Fusarium solani and of Fusarium kuroshium, causal agent of Fusarium dieback
The objective of this study was to identify bacteria or bacterial products with antagonistic activity against Fusarium solani and Fusarium kuroshium, causal agent of Fusarium dieback, by screening the rhizosphere and phyllosphere of three Lauraceae species. From 195 bacterial isolates, we identified 32 isolates that significantly reduced the growth of F. solani in vitro, which mostly belonged to bacterial taxa Bacillus, Pseudomonas and Actinobacteria. The antifungal activity of their volatile organic compounds (VOCs) was also evaluated. Bacterial strain Bacillus sp. CCeRi1-002, recovered from the rhizosphere of Aiouea effu...
Source: Microbiological Research - February 18, 2020 Category: Infectious Diseases Source Type: research

ACC deaminase in plant growth-promoting bacteria (PGPB): an efficient mechanism to counter salt stress in crops
Publication date: Available online 15 February 2020Source: Microbiological ResearchAuthor(s): Ma. del Carmen Orozco-Mosqueda, Bernard R. Glick, Gustavo SantoyoAbstractSalinity in agricultural soil is a major problem around the world, with negative consequences for the growth and production of a wide range of crops. To counteract these harmful effects, plants sometimes have bacterial partners that contain the enzyme 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, which acts by degrading ACC (the precursor of ethylene in all higher plants). The enzymatic activity of ACC deaminase results in the production of α-k...
Source: Microbiological Research - February 16, 2020 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: March 2020Source: Microbiological Research, Volume 233Author(s): (Source: Microbiological Research)
Source: Microbiological Research - February 14, 2020 Category: Infectious Diseases Source Type: research

Transcription in the acetoin catabolic pathway is regulated by AcoR and CcpA in Bacillus thuringiensis
In this study, we analyzed transcription and regulation of the aco operon in Bacillus thuringiensis (Bt). RT-PCR analysis revealed that acoABCL forms one transcriptional unit. The Sigma 54 controlled consensus sequence was located 12 bp from the acoA transcriptional start site (TSS). β-galactosidase assay revealed that aco operon transcription is induced by acetoin, controlled by sigma 54, and positively regulated by AcoR. The HTH domain of AcoR recognized and specifically bound to a 13-bp inverted repeat region that participates in 30-bp fragment mapping 81 bp upstream of the acoA TSS. The GAF domain in AcoR represse...
Source: Microbiological Research - February 14, 2020 Category: Infectious Diseases Source Type: research

Expression of attack and growth phase genes of Bdellovibrio bacteriovorus in the presence of Gram-negative and Gram-positive prey
Publication date: Available online 13 February 2020Source: Microbiological ResearchAuthor(s): M. Waso, S. Khan, W. Ahmed, W. KhanAbstractThe expression of attack phase (AP) and growth phase (GP) genes of Bdellovibrio bacteriovorus (B. bacteriovorus) was compared in the presence of Gram-negative [Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae)] and Gram-positive [Enterococcus faecium (E. faecium)] prey, using relative quantitative polymerase chain reaction (relative qPCR) assays. The genes bd0108 (pili retraction/extrusion) and merRNA (massively expressed riboswitch RNA) were highly expressed in the AP ...
Source: Microbiological Research - February 14, 2020 Category: Infectious Diseases Source Type: research

Modification of the host ubiquitome by bacterial enzymes
Publication date: Available online 11 February 2020Source: Microbiological ResearchAuthor(s): Jennifer Berglund, Rafaela Gjondrekaj, Ellen Verney, Julie Maupin-Furlow, Mariola J EdelmannAbstractAttachment of ubiquitin molecules to protein substrates is a reversible post-translational modification (PTM), which occurs ubiquitously in eukaryotic cells and controls most cellular processes. As a consequence, ubiquitination is an attractive target of pathogen-encoded virulence factors. Pathogenic bacteria have evolved multiple mechanisms to hijack the host’s ubiquitin system to their advantage. In this review, we discuss t...
Source: Microbiological Research - February 12, 2020 Category: Infectious Diseases Source Type: research

Identification of a gene encoding the last step of the L-rhamnose catabolic pathway in Aspergillus niger revealed the inducer of the pathway regulator
In this study, we identified the gene (lkaA) involved in the conversion of L-2-keto-3-deoxyrhamnonate (L-KDR) into pyruvate and L-lactaldehyde, which is the last step of the Rha pathway. Deletion of lkaA resulted in impaired growth on L-rhamnose, and potentially in accumulation of L-KDR. Contrary to ΔlraA, ΔlrlA and ΔlrdA, the expression of the Rha-responsive genes that are under control of RhaR, were at the same levels in ΔlkaA and the reference strain, indicating the role of L-KDR as the inducer of the Rha pathway regulator. (Source: Microbiological Research)
Source: Microbiological Research - February 9, 2020 Category: Infectious Diseases Source Type: research

A novel strain of endophytic Streptomyces for the biocontrol of strawberry anthracnose caused by Glomerella cingulate
In this study, we aimed to isolate and screen endophytic actinobacteria, to identify potential biocontrol agents capable of suppressing strawberry anthracnose. A total of 226 actinobacteria were successfully isolated from surface-sterilized strawberry tissues. In the first screening, 217 out of 226 actinobacteria isolates were studied for their suppression effect on strawberry anthracnose using a detached leaflet assay. It was discovered that isolates MBFA-172 and MBFA-227 markedly suppressed the development of anthracnose lesions. The efficacy of both isolates was then tested on two-month-old strawberry plug seedlings in ...
Source: Microbiological Research - February 9, 2020 Category: Infectious Diseases Source Type: research

Unraveling the metabolite signature of citrus showing defense response towards Candidatus Liberibacter asiaticus after application of endophyte Bacillus subtilis L1-21
Publication date: Available online 31 January 2020Source: Microbiological ResearchAuthor(s): Shahzad Munir, Yongmei Li, Pengfei He, Pengbo He, Ayesha Ahmed, Yixin Wu, Yueqiu HeAbstractHuanglongbing (HLB) is one of the most serious citrus diseases, caused by phloem limited endophytic bacteria Candidatus Liberibacter asiaticus (Clas), affecting worldwide citrus production. Metabolomics approaches were employed to gain insight into mechanisms involved in defense against Clas in endophyte Bacillus subtilis L1-21 treated diseased and healthy citrus plants. Using LC-ESI-MS/MS, we compared the metabolic profile of citrus plants b...
Source: Microbiological Research - February 1, 2020 Category: Infectious Diseases Source Type: research

The rhizosphere signature on the cell motility, biofilm formation and secondary metabolite production of a plant-associated Lysobacter strain
Publication date: Available online 31 January 2020Source: Microbiological ResearchAuthor(s): Francesca Brescia, Martina Marchetti-Deschmann, Rita Musetti, Michele Perazzolli, Ilaria Pertot, Gerardo PuopoloAbstractLysobacter spp. are common bacterial inhabitants of the rhizosphere of diverse plant species. However, the impact of the rhizosphere conditions on their physiology is still relatively understudied. To provide clues on the behaviour of Lysobacter spp. in this ecological niche, we investigated the physiology of L. capsici AZ78 (AZ78), a biocontrol strain isolated from tobacco rhizosphere, on a common synthetic growt...
Source: Microbiological Research - January 31, 2020 Category: Infectious Diseases Source Type: research

Shotgun Proteomic Analysis of ESBL-Producing and Non-ESBL-Producing Klebsiella Pneumoniae Clinical Isolates
Publication date: Available online 25 January 2020Source: Microbiological ResearchAuthor(s): Shymaa Enany, Samira Zakeer, Ahmed A. SayedAbstractKlebsiella pneumoniae is a pathogenic bacterium that is responsible for a wide range of infections in humans. An increased rate of infections caused by multi-drug-resistant K. pneumoniae has been noted in the last two decades. The association between antimicrobial resistance and virulence is an important topic of study. Genomic tools have been used widely for the detection of virulence. In our study, we used proteomic analysis with mass spectrometry and bioinformatics tools to expl...
Source: Microbiological Research - January 27, 2020 Category: Infectious Diseases Source Type: research

Induction of drought tolerance in tomato upon the application of ACC deaminase producing plant growth promoting rhizobacterium Bacillus subtilis Rhizo SF 48
Publication date: Available online 25 January 2020Source: Microbiological ResearchAuthor(s): H.G. Gowtham, S. Brijesh Singh, M. Murali, N. Shilpa, Melvin Prasad, Mohammed Aiyaz, K.N. Amruthesh, S.R. NiranjanaAbstractA total of ten 1-aminocyclopropane-1-carboxylate (ACC) deaminase producing PGPR isolates were selected and evaluated for the induction of drought stress tolerance in tomato. Among the selected PGPR, maximum seed (laboratory) and plant growth promotion (greenhouse) was observed in tomato seeds bacterized with Bacillus subtilis Rhizo SF 48. The genomic study confirmed the presence of ACC deaminase gene in Rhizo S...
Source: Microbiological Research - January 27, 2020 Category: Infectious Diseases Source Type: research

Metabolite profiling reveals a complex response of plants to application of plant growth-promoting endophytic bacteria
Publication date: Available online 25 January 2020Source: Microbiological ResearchAuthor(s): Ahmad Mahmood, Ryota KataokaAbstractEndophytic bacteria have been explored for their role in plant growth promotion, however, not much has been explored in cucumber. The metabolomic response of plants to application of such microbes also remains largely unknown. Thus, we investigated the application of endophytic bacteria to cucumber to infer their role in plant growth promotion and document metabolome response. The lowest healthy leaf-stalks were sampled from four differently sourced cucumber plants, and endophytic bacteria were i...
Source: Microbiological Research - January 27, 2020 Category: Infectious Diseases Source Type: research

The orphan histidine kinase PdtaS-p regulates both morphological differentiation and antibiotic biosynthesis together with the orphan response regulator PdtaR-p in Streptomyces
In this study, based on bioinformatics analysis, we identify the cognate RR PdtaR-p of PdtaS-p by phenotype similarity of gene deletion mutants as well as in vitro phosphor-transfer assay. We show that the mutants (ΔpdtaR-p and ΔpdtaS-p) exhibit almost the same phenotypical changes, showing a bald phenotype on MS agar and reduced pristinamycin biosynthesis. Further phosphor-transfer assay indicates that the phosphoryl group of HK PdtaS-p can be specifically transferred to RR PdtaR-p. Compared with the majority of RRs that harbor DNA-binding domains, PdtaR-p contains a putative ANTAR RNA-binding domain involved ...
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research

Genotyping and population diversity of Bacillus anthracis in China based on MLVA and canSNP analysis
In this study, 191 isolates were selected to assess the diversity of B. anthracis in China. Five isolates were confirmed not to be B. anthracis by clustered regularly interspaced short palindromic repeat analysis, while the remaining 186 isolates were typed using canonical SNP (canSNP) and VNTR analyses. Five sublineages/subgroups, A.Br.001/002, A.Br.Vollum, A.Br.Aust.94, A.Br.Ames, and A.Br.008/009, were detected based on 13 canSNP sites. The 186 isolates were further assigned 114 sequence types based on 27 VNTR loci, with major branches correlating with the canSNP analysis. We then used a simplified multiple-locus variab...
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research

Pantoea Natural Product 3 is encoded by an eight-gene biosynthetic gene cluster and exhibits antimicrobial activity against multi-drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa
Publication date: Available online 15 January 2020Source: Microbiological ResearchAuthor(s): Ashley N. Williams, John StavrinidesAbstractMulti-drug resistant Acinetobacter baumannii and Pseudomonas aeruginosa continue to pose a serious health threat worldwide. Two Pantoea agglomerans strains, 3581 and SN01080, produce an antibiotic effective against these pathogens. To identify the antibiotic biosynthetic gene clusters, independent genetic screens were conducted for each strain using a mini-Tn5 transposon, which resulted in the identification of the same conserved eight-gene cluster. We have named this antibiotic Pantoea N...
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research

Diversity of endophytic plant-growth microorganisms from Gentianella weberbaueri and Valeriana pycnantha, highland Peruvian medicinal plants
Publication date: Available online 15 January 2020Source: Microbiological ResearchAuthor(s): Rocío Ulloa-Muñoz, Percy Olivera-Gonzales, Alberto Castañeda-Barreto, Gretty K. Villena, Carmen Tamariz-AngelesAbstractMicrobial diversity in Peruvian mountain areas is poorly know, specially endophytic microorganisms of medicinal native plants from the Cordillera Blanca. So, nine bacterial and six fungal species were isolated from Gentianella weberbaueri and Valeriana pycnantha. According to 16S rDNA analysis, bacterial strains belong to genera Rahnella, Pseudomonas, Serratia, Rouxiella, and Bacillus; while IT...
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: February 2020Source: Microbiological Research, Volume 232Author(s): (Source: Microbiological Research)
Source: Microbiological Research - January 16, 2020 Category: Infectious Diseases Source Type: research

Genomic and Phenomic Analysis of a Marine Bacterium, Photobacterium marinum J15
Publication date: Available online 9 January 2020Source: Microbiological ResearchAuthor(s): Noordiyanah Nadhirah Roslan, Mohamad Syazwan Ngalimat, Adam Thean Chor Leow, Siti Nurbaya Oslan, Syarul Nataqain Baharum, Suriana SabriAbstractPhotobacterium species are widely distributed in the marine environment. The overall metabolism of this genus remains largely unknown. In order to improve our knowledge on this bacterium, the relationship between the genome and phenome of the Photobacterium isolate was analyzed. The cream colored, Gram-negative, rod-shaped and motile bacterial strain, J15, was isolated from marine water of Ta...
Source: Microbiological Research - January 10, 2020 Category: Infectious Diseases Source Type: research

Beneficial effect of GABA-rich fermented milk on insomnia involving regulation of gut microbiota
In this study, milk was fermented with Lactobacillus brevis DL1-11, a strain with high GABA-producing capacity. The potential beneficial effects of this fermented milk on anxiety and sleep quality were evaluated in animal experiments. Sixty mice were divided into control, non-GABA fermented milk (NGFM), low-dose GABA fermented milk (LGFM, 8.83 mg/kg.bw), medium-dose GABA fermented milk (MGFM, 16.67 mg/kg.bw), high-dose GABA fermented milk (HGFM, 33.33 mg/kg.bw) and diazepam groups. The results of open field test and elevated plus-maze test indicated decreases in anxiety behavior after oral HGFM adminis...
Source: Microbiological Research - January 7, 2020 Category: Infectious Diseases Source Type: research

Revealing the impact of global mass bleaching on coral microbiome through 16S rRNA gene-based metagenomic analysis
Publication date: Available online 31 December 2019Source: Microbiological ResearchAuthor(s): Ramu Meenatchi, Thangadurai Thinesh, Pownraj Brindangnanam, Saqib Hassan, George Seghal Kiran, Joseph SelvinAbstractCoral bleaching, a phenomenon by which the expulsion of corals’ alveolate endosymbiont (zooxanthellae) occurs when experiencing thermal stress is the major cause for devastation of corals. However, apart from this obligate symbiont of Scleractinian corals, there are different kinds of microbes that exist as stable, transient or sporadic members of the holobiont which resides within various microhabitats in the ...
Source: Microbiological Research - January 1, 2020 Category: Infectious Diseases Source Type: research

Bacterial line of defense in Dirinaria lichen from two different ecosystems: First genomic insights of its mycobiont Diriniria sp. GBRC AP01
Publication date: Available online 28 December 2019Source: Microbiological ResearchAuthor(s): Apurvasinh C. Puvar, Neelam M. Nathani, Inayatullah Shaikh, Arpan D. Bhatt, Poonam Bhargava, Chaitanya G. Joshi, Madhvi N. JoshiAbstractLichens have been widely studied for their symbiotic properties and for the secondary metabolites production by its fungal symbiont. Recent molecular studies have confirmed coexistence of bacteria along with the fungal and algal symbionts. Direct nucleic acid study by -omics approaches is providing better insights into their structural and functional dynamics. However, genomic analysis of individu...
Source: Microbiological Research - December 29, 2019 Category: Infectious Diseases Source Type: research

Selected isolates of Trichoderma gamsii induce different pathways of systemic resistance in maize upon Fusarium verticillioides challenge
Publication date: Available online 19 December 2019Source: Microbiological ResearchAuthor(s): Stefania Galletti, Roberta Paris, Stefano CianchettaAbstractThe pink ear rot is one of the most damaging maize diseases, caused by the mycotoxigenic fungal pathogen, Fusarium verticillioides. The application of biological control agents, like antagonistic and/or resistance inducer microorganisms, is an option to reduce fungal infection and kernel contamination in a sustainable and environmentally friendly way. It is well known that Trichoderma species are non-pathogenic fungi able to antagonize plant pathogens and to induce system...
Source: Microbiological Research - December 21, 2019 Category: Infectious Diseases Source Type: research

Retraction notice to “Dissection of ToxR-dependent and ToxR-independent stress-regulated pathways in Vibrio parahaemolyticus” Microbiol. Res. 223–225 (2019) 79–87
Publication date: Available online 19 December 2019Source: Microbiological ResearchAuthor(s): Lingzhi Li, Miaomiao Gao, Tianyu Lu, Dan Gu (Source: Microbiological Research)
Source: Microbiological Research - December 19, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: January 2020Source: Microbiological Research, Volume 231Author(s): (Source: Microbiological Research)
Source: Microbiological Research - December 18, 2019 Category: Infectious Diseases Source Type: research

Isolation and characterization of Burkholderia cenocepacia CR318, a phosphate solubilizing bacterium promoting corn growth
Publication date: Available online 14 December 2019Source: Microbiological ResearchAuthor(s): Man You, Shumei Fang, Jacqueline MacDonald, Jianping Xu, Ze-Chun YuanAbstractPlant-growth promoting rhizobacteria benefit crop health and growth through various mechanisms including phosphate and potassium solubilisation, and antimicrobial activity. Previously, we sequenced the genome of bacterial strain Burkholderia cenocepacia CR318, which was isolated from the roots of the starch corn (Zea mays L.) in London, Ontario, Canada. In this work, the species identity of this isolate is confirmed by recA phylogeny and in silico DNA-DNA...
Source: Microbiological Research - December 15, 2019 Category: Infectious Diseases Source Type: research

Isolation and characterization of psychrophilic and psychrotolerant plant-growth promoting microorganisms from a high-altitude volcano crater in Mexico
This study offers the possibility of using these strains as an additive to improve culture conditions of S. lycopersicum in a more environmentally compatible way. This is the first study to propose psychrophilic/psychrotolerant yeasts, as plant growth promoting microbes. (Source: Microbiological Research)
Source: Microbiological Research - December 15, 2019 Category: Infectious Diseases Source Type: research

Isolation of bacteria at different points of Pleurotus ostreatus cultivation and their influence in mycelial growth
Publication date: Available online 13 December 2019Source: Microbiological ResearchAuthor(s): Christian Suarez, Stefan Ratering, Victoria Weigel, Julia Sacharow, Jackeline Bienhaus, Janine Ebert, Anika Hirz, Martin Rühl, Sylvia SchnellABSTRACTPleurotus ostreatus is one of the most cultivated edible mushrooms worldwide and few approaches have been done to analyze bacterial influence during its cultivation. Therefore, bacteria from commercial spawn, mycelial-colonized straw and fruiting bodies from healthy productive samples were counted, isolated and tested for their mycelial growth promoting ability. Bacterial cell nu...
Source: Microbiological Research - December 14, 2019 Category: Infectious Diseases Source Type: research

Characterization the role of GacA-dependent small RNAs and RsmA family proteins on 2,4-diacetylphloroglucinol production in Pseudomonas fluorescens 2P24
Publication date: Available online 9 December 2019Source: Microbiological ResearchAuthor(s): Yang Zhang, Bo Zhang, Xiaogang Wu, Li-Qun ZhangAbstractPseudomonas fluorescens 2P24 is a plant-beneficial rhizobacteria that controls many root diseases caused by soil-borne pathogens, and the production of the antibiotic compound 2,4-diacetylphloroglucinol (2,4-DAPG) is essential for its biocontrol ability. In the present study, we investigated the regulatory mechanism acting on the production of 2,4-DAPG by the GacA-dependent small non-coding RNAs (sRNAs) and RsmA/E proteins in strain 2P24. Our results showed that the GacS-GacA s...
Source: Microbiological Research - December 9, 2019 Category: Infectious Diseases Source Type: research

The cell surface adhesins of Mycobacterium tuberculosis
Publication date: Available online 9 December 2019Source: Microbiological ResearchAuthor(s): Vivek Vinod, Sukhithasri Vijayrajratnam, Anil Kumar Vasudevan, Raja BiswasAbstractBacterial cell surface adhesins play a major role in facilitating host colonization and subsequent establishment of infection. The surface of Mycobacterium tuberculosis, owing to the complex architecture of its cell envelope, expresses numerous adhesins with varied chemical nature, including proteins, lipids, lipoproteins, glycoproteins and glycopolymers. Studies on mycobacterial adhesins show that they bind with multifarious host receptors and extrac...
Source: Microbiological Research - December 9, 2019 Category: Infectious Diseases Source Type: research

Community structures of the rhizomicrobiomes of cultivated and wild soybeans in their continuous cropping
In this study, we simulated monocropping of cultivated and wild soybeans under greenhouse conditions to investigate the rhizomicrobiomes of both soybeans. Results indicated that the bacterial community structure still maintained a changing trend after four continuous planting seasons, while fungal community structure showed a stable trend as indicated by the high similarity in the fungal community structure between the third and fourth planting rotations in both soybeans. In addition, by comparing the continuous cropping of the two soybeans, we found different fungal groups in their rhizospheres between the wild and cultiv...
Source: Microbiological Research - December 8, 2019 Category: Infectious Diseases Source Type: research

Functional Characterization of Potential PGPR Exhibiting Broad-spectrum Antifungal activity
This study describes the biocontrol potential ofrhizobacteria against a range of fungal phytopathogens. Out of 227 bacteria isolated from the rhizosphere of maize, rice, wheat, potato, sunflower and soybean crops cultivated in different agro-ecological regions of Pakistan, 48 exhibited>60% antifungal activity againstFusarium oxysporum, Fusarium moniliforme, Rhizoctonia solani, Colletotrichum gloeosporioides, Colletotrichum falcatum, Aspergillus niger, and Aspergillus flavus. The rhizobacteria inhibiting> 65% pathogen growth were selected for detailed molecular and in planta studies most of which were identified as Ps...
Source: Microbiological Research - December 2, 2019 Category: Infectious Diseases Source Type: research

Rhizobacteria-induced systemic tolerance against drought stress in Sorghum bicolor (L.) Moench
Publication date: Available online 30 November 2019Source: Microbiological ResearchAuthor(s): René Carlson, Fidele Tugizimana, Paul A. Steenkamp, Ian A. Dubery, Ahmed Idris Hassen, Nico LabuschagneAbstractInduction of systemic tolerance in sorghum [Sorghum bicolor (L.) Moench] against drought stress was studied by screening a large collection of rhizobacterial isolates for their potential to exhibit this essential plant growth-promoting trait. This was done by means of a greenhouse assay that measured the relative change in both plant height and -biomass (roots and shoots) between rhizobacteria-primed versus non-pri...
Source: Microbiological Research - December 2, 2019 Category: Infectious Diseases Source Type: research

Inhibition of biofilm- and hyphal- development, two virulent features of Candida albicans by secondary metabolites of an endophytic fungus Alternaria tenuissima having broad spectrum antifungal potential
Publication date: Available online 28 November 2019Source: Microbiological ResearchAuthor(s): Sohini Chatterjee, Ranjan Ghosh, Narayan Chandra MandalAbstractFungal resistance against frequently used antifungal medicines used for invasive candidiasis and other fungal infections is directing scientist for searching and developing novel antifungal drugs. An endophytic fungal strain Alternaria tenuissima OE7 has been isolated from leaves of Ocimum tenuiflorum L. which showed antifungal activity against numbers of human pathogenic fungi including Trichophyton rubrum, Microsporum gypseum, Aspergillus parasiticus, A. flavus, A. f...
Source: Microbiological Research - November 30, 2019 Category: Infectious Diseases Source Type: research

Ecological impact of a rare sugar on grapevine phyllosphere microbial communities
Publication date: Available online 26 November 2019Source: Microbiological ResearchAuthor(s): Michele Perazzolli, Andrea Nesler, Oscar Giovannini, Livio Antonielli, Gerardo Puopolo, Ilaria PertotAbstractPlants host a complex microbiota inside or outside their tissues, and phyllosphere microorganisms can be influenced by environmental, nutritional and agronomic factors. Rare sugars are defined as monosaccharides with limited availability in nature and they are metabolised by only few certain microbial taxa. Among rare sugars, tagatose (TAG) is a low-calories sweetener that stimulates and inhibits beneficial and pathogenic b...
Source: Microbiological Research - November 26, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: January 2020Source: Microbiological Research, Volume 230Author(s): (Source: Microbiological Research)
Source: Microbiological Research - November 20, 2019 Category: Infectious Diseases Source Type: research

Identification of mimp-associated effector genes in Fusarium oxysporum f. sp. cubense race 1 and race 4 and virulence confirmation of a candidate effector gene
In this study, genome-wide effector gene identification was performed in Foc race 1 (Foc 1) and Foc race 4 (Foc 4) based on the context association between the effector genes and the transposable element mimp. A total of 20 candidate effector genes were identified, of which 3 were Foc 1-specific, 6 were Foc 4-specific, and 11 were present in both Foc 1 and Foc 4. Most genes (14 out of 20) showed a significant transcriptional burst in planta compared with in-culture conditions, from more than 10-fold to 1,617-fold, and at the highest 32,725-fold. In addition to Foc 1- and Foc 4-specific genes, the genes Foc 283, Foc 495, an...
Source: Microbiological Research - November 19, 2019 Category: Infectious Diseases Source Type: research

Molecular and Biotechnological Aspects of Secondary Metabolites in Actinobacteria
Publication date: Available online 12 November 2019Source: Microbiological ResearchAuthor(s): Richa Salwan, Vivek SharmaAbstractThe ability to produce plethora of secondary metabolites and enzymes for pharmaceutical, agricultural and biotechnological applications make actinobacteria one of the most explored microbes among prokaryotes. The secondary metabolites and lytic enzymes of actinobacteria are known for their role in various physiological, cellular and biological processes including environmental sensing, mineral acquisition and recycling, and establishing social communication. In addition, the basic scaffold of seco...
Source: Microbiological Research - November 14, 2019 Category: Infectious Diseases Source Type: research

Biochar amendment controlled bacterial wilt through changing soil chemical properties and microbial community
This study revealed the potential of biochar in control of bacterial wilt. (Source: Microbiological Research)
Source: Microbiological Research - November 13, 2019 Category: Infectious Diseases Source Type: research

Mating-type switching and mating-type gene array expression in the methylotrophic yeast Ogataea thermomethanolica TBRC656
In this study, we focused on mating-type switching and gene expression in order to elucidate its sexual life cycle and establish genetic approaches applicable for the strain. A putative mating-type gene cluster was identified in TBRC656 that is syntenic to the cluster in Ogataea parapolymorpha DL-1 (previously named Hansenula polymorpha). Like DL-1, TBRC656 possesses two mating loci, namely MATa and MATα, and also shows flip-flop mating-type switching. Interestingly, unlike any other methylotrophic yeast, TBRC656 robustly switched mating type during late growth in rich medium (YPD). Under nutrient depletion, mating-t...
Source: Microbiological Research - November 11, 2019 Category: Infectious Diseases Source Type: research

Colonization of the Gut Microbiota of Honey Bee (Apis mellifera) Workers at Different Developmental Stages
In this study, we explored the dynamic changes of the gut microbiota of A. mellifera at different developmental stages covering the whole life cycle using high-throughput 16S rRNA gene sequencing. The results indicated that the core (shared) gut microbiota changes significantly among different developmental stages. The diversity of the bacterial community in workers among different ages was significantly different. In addition, by comparing the core gut microbiota among different-aged workers, we found that newly emerged workers had fewer core microbiota. Three genera, Gilliamella, Frischella, and Snodgrassella, were signi...
Source: Microbiological Research - November 7, 2019 Category: Infectious Diseases Source Type: research

Isolation of Trichoderma from forestry model base and the antifungal properties of isolate TpsT17 toward Fusarium oxysporum
Publication date: Available online 5 November 2019Source: Microbiological ResearchAuthor(s): Chang Zhou, Ruiting Guo, Shida Ji, Haijuan Fan, Jinjie Wang, Yucheng Wang, Zhihua LiuAbstractEleven soil samples were collected from different plantations at the Forestry Model Base, Northeast Forestry University, China (45°43ʹ10″ N, 126°37ʹ15″ E), and 122 Trichoderma strains (T1–T122) were isolated. Nine Trichoderma species were identified based on morphological and molecular classification methods. The diversity of woody fungi was analyzed based on the type and quantity of Trichoderma spp. in the soil ...
Source: Microbiological Research - November 7, 2019 Category: Infectious Diseases Source Type: research

The potential of endophytic fungi isolated from cucurbit plants for biocontrol of soilborne fungal diseases of cucumber
In this study, 82 endophytic fungal strains had good disease suppressive effects and no obvious adverse effects on cucumber growth, and 35 of them showed obvious growth-promoting effects, which suggested that endophytic fungi from cucurbit plants have excellent potential for plant disease control. (Source: Microbiological Research)
Source: Microbiological Research - November 5, 2019 Category: Infectious Diseases Source Type: research

Illumina sequencing of 16S rRNA tag shows disparity in rhizobial and non-rhizobial diversity associated with root nodules of mung bean (Vigna radiata L.) growing in different habitats in Pakistan
Publication date: Available online 23 October 2019Source: Microbiological ResearchAuthor(s): Sughra Hakim, Babur S. Mirza, Asma Imran, Ahmad Zaheer, Sumera Yasmin, Fathia Mubeen, Joan E. Mclean, M. Sajjad MirzaAbstractIn Rhizobium-legume symbiosis, the nodule is the most frequently studied compartment, where the endophytic/symbiotic microbiota demands critical investigation for development of specific inocula. We identified the bacterial diversity within root nodules of mung bean from different growing areas of Pakistan using Illumina sequencing of 16S rRNA gene. We observed specific OTUs related to specific site where Bra...
Source: Microbiological Research - October 24, 2019 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: December 2019Source: Microbiological Research, Volume 229Author(s): (Source: Microbiological Research)
Source: Microbiological Research - October 23, 2019 Category: Infectious Diseases Source Type: research

CpALS4770 and CpALS4780 contribution to the virulence of Candida parapsilosis
Publication date: Available online 18 October 2019Source: Microbiological ResearchAuthor(s): Marina Zoppo, Mariagrazia Di Luca, Mauro Franco, Cosmeri Rizzato, Antonella Lupetti, Annarita Stringaro, Flavia De Bernardis, Christoph Schaudinn, M. Inmaculada Barrasa, Daria Bottai, Valmik K. Vyas, Arianna TavantiAbstractThe ability of yeast to adhere to biotic and abiotic surfaces represents an essential trait during the early stages of infection. Agglutinin-like sequence (Als) cell-wall proteins play a key role in adhesion of Candida species. Candida parapsilosis genome encompasses 5 ALS members, of which only the role of CPAR2...
Source: Microbiological Research - October 20, 2019 Category: Infectious Diseases Source Type: research

Arbuscular mycorrhizal fungi (Glomus intraradices) and diazotrophic bacterium (Rhizobium BMBS) primed defense in blackgram against herbivorous insect (Spodoptera litura) infestation
Publication date: Available online 17 October 2019Source: Microbiological ResearchAuthor(s): Selvaraj Anandakumar, Thangavel Kalaiselvi, Uthandi SivakumarAbstractIn the changing scenario of agriculture, plants are exposed to various biotic and abiotic stresses. Induction of both constitutive and inducible defense systems was noticed in plants exposed to stress. As a major defense response, production of phenolics and superoxide radicals quenching enzymes is accelerated in plants under stress. These metabolites production intensified further when arbuscular mycorrhizal fungi (AMF) infected plants are subjected to stress. Wi...
Source: Microbiological Research - October 18, 2019 Category: Infectious Diseases Source Type: research

Molecular and morphological characterization of rice phylloplane fungi and determination of the antagonistic activity against rice pathogens
Publication date: Available online 15 October 2019Source: Microbiological ResearchAuthor(s): Amanda A. Chaibub, Thatyane P. de Sousa, Leila G. de Araújo, Marta Cristina C. de FilippiAbstractCladosporium spp. is a cosmopolitan fungal genus. In the literature, it has been reported as a biological agent for controlling several plant diseases, but its mechanism of action has never been clarified. The present study aims to identify Cladosporium spp. based on the DNA phylogeny of nine isolates obtained from the phylloplane of rice and their potential antagonistic activity against the main fungal pathogens that affect rice...
Source: Microbiological Research - October 17, 2019 Category: Infectious Diseases Source Type: research