Editorial Board
Publication date: January 2019Source: Microbiological Research, Volume 218Author(s): (Source: Microbiological Research)
Source: Microbiological Research - November 17, 2018 Category: Infectious Diseases Source Type: research

Retraction notice to "The opportunistic human fungal pathogen Candida albicans promotes the growth and proliferation of commensal Escherichia coli through an iron-responsive pathway" [Microbiological Research 207 (2018) 232-239]
Publication date: January 2019Source: Microbiological Research, Volume 218Author(s): Shanshan Li, Xiaoyu Yu, Wenjuan Wu, Daniel Z. Chen, Ming Xiao, Xinhua Huang (Source: Microbiological Research)
Source: Microbiological Research - November 17, 2018 Category: Infectious Diseases Source Type: research

Bacterial chemotaxis coupling protein: Structure, function and diversity
Publication date: Available online 6 November 2018Source: Microbiological ResearchAuthor(s): Zhiwei Huang, Xiaoyue Pan, Nan Xu, Minliang GuoAbstractIn most signal transduction systems, coupling or scaffold proteins establish crucial connections between receptors and histidine kinases. These connections are important for signal transduction. The bacterial chemotaxis system is a canonical signal transduction system that relies on coupling proteins. The coupling proteins in the chemotaxis system have two architectures: CheW or CheV. In a typical chemotaxis signal transduction system, two CheW coupling protein molecules bridge...
Source: Microbiological Research - November 7, 2018 Category: Infectious Diseases Source Type: research

Physiological response of Lactuca sativa exposed to 2-nonanone emitted by Bacillus sp. BCT9
In this study, 2-day-old L. sativa were exposed to 2-nonanone for 10 days under two delivery systems: 1) 2-nonanone (abrupt delivery) and 2) 2-nonanone + lanolin (controlled delivery). The X-ray elemental microanalysis, scanning electron and confocal laser microscopies techniques were used to evaluate physiological changes “in vivo” conditions. The results indicated that 2-nonanone increased root and shoot length independently of 2-nonanone delivery system after 7 days of exposition. Additionally, 2-nonanone elicited the increase of anthocyanin and not affects chlorophyll content and electrolyte l...
Source: Microbiological Research - November 4, 2018 Category: Infectious Diseases Source Type: research

Development of low-cost formulations of plant growth-promoting bacteria to be used as inoculants in beneficial agricultural technologies
Publication date: Available online 2 November 2018Source: Microbiological ResearchAuthor(s): Constanza Belén Lobo, María Silvina Juárez Tomás, Emilce Viruel, Marcela Alejandra Ferrero, María Ester LuccaAbstractPhosphorus is one of the main macronutrients for plant development. Despite its large deposits in soils, it is scarcely available for plants. Phosphate-solubilizing bacteria, belonging to the group of plant growth-promoting rhizobacteria (PGPR), are capable of mobilizing deposits of insoluble phosphates in the soil. The use of PGPR as inoculants provides an environmentally sustainab...
Source: Microbiological Research - November 3, 2018 Category: Infectious Diseases Source Type: research

Current situation of biofuel production and its enhancement by CRISPR/Cas9-mediated genome engineering of microbial cells
Publication date: Available online 2 November 2018Source: Microbiological ResearchAuthor(s): Muhammad Rizwan Javed, Muhammad Noman, Muhammad Shahid, Temoor Ahmed, Mohsin Khurshid, Muhammad Hamid Rashid, Muhammad Ismail, Maria Sadaf, Fahad KhanAbstractGeopolitical and economic factors have motivated the scientific community to utilize renewable energy resources. In addition to the modifications in major steps and processes of biofuel production, manipulation of microbial genome engineering tools is essential in order to find sustainable solution of continuous depletion of fossil-fuels. Recently, the CRISPR (clustered regula...
Source: Microbiological Research - November 3, 2018 Category: Infectious Diseases Source Type: research

The Potential of Bacilli Rhizobacteria for Sustainable Crop Production and Environmental Sustainability
Publication date: Available online 2 November 2018Source: Microbiological ResearchAuthor(s): B.N. Aloo, B.A. Makumba, E.R. MbegaAbstractConventional agricultural practices often rely on synthetic fertilizers and pesticides which have immense and adverse effects on humans, animals and environments. To minimize these effects, scientists world over are now deeply engaged in finding alternative approached for crop production which are less dependent on chemical inputs. One such approach is the use of rhizospheric bacteria as vital components of soil fertility and plant growth promotion (PGP) through their direct and indirect p...
Source: Microbiological Research - November 3, 2018 Category: Infectious Diseases Source Type: research

Far-reaching cellular consequences of tat deletion in Escherichia coli revealed by comprehensive proteome analyses
In this study, we investigated the influence of the deletion of all active Tat pathway components on the E. coli cells. The comprehensive proteomic analysis revealed activation of several stress responses and experimentally confirmed the dependence of certain proteins on the Tat system for export. We observed that a tat deletion triggers protein aggregation, membrane vesiculation, synthesis of colanic acid and biofilm formation. Furthermore, the mislocalization of Tat-dependent proteins disturbs iron and molybdenum homeostasis and impairs the cell envelope integrity. The results show that the functional Tat pathway is impo...
Source: Microbiological Research - November 2, 2018 Category: Infectious Diseases Source Type: research

Transcriptome analysis of hopanoid deficient mutant of Rhodopseuodomonas palustris TIE-1
Publication date: Available online 29 October 2018Source: Microbiological ResearchAuthor(s): Tushar D. Lodha, Indu B., Sasikala Ch., Ramana Ch.V.AbstractAll three domains of life have an ordered plasma membrane which is pivotal in the selective fitness of primitive life. Like cholesterol in eukaryotes, hopanoids are important in bacteria to modulate membrane order. Hopanoids are pentacyclic triterpenoid lipids biosynthesised in many eubacteria, few ferns and lichens. Hopanoid modulates outer membrane order and hopanoid deficiency results in the weakened structural integrity of the membrane which may intern affect the other...
Source: Microbiological Research - October 30, 2018 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: December 2018Source: Microbiological Research, Volume 217Author(s): (Source: Microbiological Research)
Source: Microbiological Research - October 30, 2018 Category: Infectious Diseases Source Type: research

The histidine kinase slnCl1 of Colletotrichum lindemuthianum as a pathogenicity factor against Phaseolus vulgaris L
Publication date: Available online 28 October 2018Source: Microbiological ResearchAuthor(s): Guilherme Bicalho Nogueira, Leandro Vieira dos Santos, Casley Borges de Queiroz, Thamy Lívia Ribeiro Corrêa, Renato Pedrozo Menicucci, Denise Mara Soares Bazzolli, Elza Fernandes de Araújo, Marisa Vieira de QueirozAbstractColletotrichum lindemuthianum, the causal agent of anthracnose, is responsible for significant damage in the common bean (Phaseolus vulgaris L.). Unraveling the genetic mechanisms involved in the plant/pathogen interaction is a powerful approach for devising efficient methods to control this di...
Source: Microbiological Research - October 29, 2018 Category: Infectious Diseases Source Type: research

Plant-microbe interactions endorse growth by uplifting microbial community structure of Bacopa monnieri rhizosphere under nematode stress
Publication date: Available online 26 October 2018Source: Microbiological ResearchAuthor(s): Rupali Gupta, Akanksha Singh, Madhumita Srivastava, Karuna Shanker, Rakesh PandeyAbstractThe modification of rhizosphere microbial diversity and ecological processes are of rising interest as shifting in microbial community structure impacts the mutual role of host–microbe interactions. Nevertheless, the connection between host-microbial community diversity, their function under biotic stress in addition to their impact on plant performances is poorly understood. The study was designed with the aim to analyze the tripartite i...
Source: Microbiological Research - October 27, 2018 Category: Infectious Diseases Source Type: research

Deletion of the epigenetic regulator GcnE in Aspergillus niger FGSC A1279 activates the production of multiple polyketide metabolites
This study found that inactivation of GcnE activated the production of secondary metabolites in A. niger. The biosynthetic pathway for nigerpyrone and its derivatives was identified and characterized via gene knockout and complementation experiments. A biosynthetic model of this group of pyran-based fungal metabolites was proposed. (Source: Microbiological Research)
Source: Microbiological Research - October 16, 2018 Category: Infectious Diseases Source Type: research

Corrigendum to “Streptomyces puniceus strain AS13., Production, characterization and evaluation of bioactive metabolites: A new face of dinactin as an antitumor antibiotic” [Microbiological Research 207, (2018)196-202]
Publication date: Available online 9 October 2018Source: Microbiological ResearchAuthor(s): Aehtesham Hussain, Muzafar Ahmad Rather, Mohd Saleem Dar, N A Dangroo, Mushtaq A. Aga, Arem qayum, Aabid Manzoor Shah, Zahoor Ahmad, Mohd Jamal Dar, Qazi Parvaiz Hassan (Source: Microbiological Research)
Source: Microbiological Research - October 10, 2018 Category: Infectious Diseases Source Type: research

Partner-triggered proteome changes in the cell wall of Bacillus sonorensis and roots of groundnut benefit each other
Publication date: Available online 9 October 2018Source: Microbiological ResearchAuthor(s): Sravani Ankati, T. Swaroopa Rani, Appa Rao PodileAbstractPlant growth promoting rhizobacteria (PGPR) promote plant growth and activate defense response against phytopathogens. At the subcellular level plant-PGPR interaction is less understood, which would be essential for future improvement(s) of PGPR formulations. In a rigorous screening process, that also involved efficient PGPR strains, Bacillus sonorensis RS4 was selected to study partner-triggered interactions. The potential of B. sonorensis RS4 to improve growth of groundnut, ...
Source: Microbiological Research - October 10, 2018 Category: Infectious Diseases Source Type: research

Antimicrobial, plant growth-promoting and genomic properties of the peanut endophyte Bacillus velezensis LDO2
In this study, the peanut endophytic bacterium LDO2 was evaluated for the potential of peanut pathogens inhibition and growth-promotion, and the genetic mechanisms were explored by genome mining. Strain LDO2 significantly inhibited the growth of peanut pathogenic fungi and pathogenic bacteria, and specifically, it showed pronounced inhibition on mycelia growth of Aspergillus flavus mycelia and caused mycelial deformity. Gene clusters responsible for antifungal metabolites (fengycin, surfactin, bacilysin) and antibacterial metabolites (butirosin, bacillaene, difficidin, macrolactin, surfactin, bacilysin) were identified. St...
Source: Microbiological Research - October 10, 2018 Category: Infectious Diseases Source Type: research

Isolation and characterization of endophytes from nodules of Mimosa pudica with biotechnological potential
Publication date: Available online 6 October 2018Source: Microbiological ResearchAuthor(s): Ricardo Sánchez-Cruz, Irán Tapia Vázquez, Ramón Alberto Batista-García, Erick Williams Méndez Santiago, María del Rayo Sánchez Carbente, Alfonso Leija, Verónica Lira-Ruan, Gerogina Hernández, Arnoldo Wong-Villarreal, Jorge Luis Folch-MallolAbstractLegumes establish symbiotic relationships with different microorganisms, which could function as plant growth promotion microorganisms (PGPM). The finding of new PGPM strains is important to increase plant production avo...
Source: Microbiological Research - October 7, 2018 Category: Infectious Diseases Source Type: research

Environmental conditions shape the biofilm of the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125
Publication date: Available online 6 October 2018Source: Microbiological ResearchAuthor(s): Annarita Ricciardelli, Angela Casillo, Alessandro Vergara, Nicole Balasco, Maria Michela Corsaro, Maria Luisa Tutino, Ermenegilda ParrilliAbstractBiofilms are the most widely distributed and successful microbial modes of life. The capacity of bacteria to colonize surfaces provides stability in the growth environment, allows the capturing of nutrients and affords protection from a range of environmental challenges and stress. Bacteria living in cold environments, like Antarctica, can be found as biofilms, even though the mechanisms o...
Source: Microbiological Research - October 7, 2018 Category: Infectious Diseases Source Type: research

Recombineering Pseudomonas protegens CHA0: An innovative approach that improves nitrogen fixation with impressive bactericidal potency
Publication date: Available online 6 October 2018Source: Microbiological ResearchAuthor(s): Fangnan Yu, Xiaoshu Jing, Xiaochen Li, Hailong Wang, Hanna Chen, Lin Zhong, Jia Yin, Deng Pan, Yulong Yin, Jun Fu, Liqiu Xia, Xiaoying Bian, Qiang Tu, Youming ZhangAbstractPseudomonas protegens CHA0 is a well-characterized, root-colonizing bacterium with broad-spectrum biocontrol ability. Therefore, it has a great potential to curb plant diseases and to partly replace synthetic chemical pesticides that are harmful to humans. Here, we obtained the multifunctional mutant CHA0-ΔretS-Nif via Red/ET recombineering technology. After...
Source: Microbiological Research - October 7, 2018 Category: Infectious Diseases Source Type: research

Helicobacter pylori evasion strategies of the host innate and adaptive immune responses to survive and develop gastrointestinal disease
Publication date: Available online 6 October 2018Source: Microbiological ResearchAuthor(s): Ahmad Karkhah, Soheil Ebrahimpour, Maryam Rostamtabar, Veerendra Koppolu, Sorena Darvish, Veneela Krishna Rekha Vasigala, Majid Validi, Hamid Reza NouriAbstractHelicobacter pylori (H. pylori) is a bacterial pathogen that resides in more than half of the human population and has co-evolved with humans for more than 58000 years. This bacterium is orally transmitted during childhood and is a key cause of chronic gastritis, peptic ulcers and two malignant cancers including MALT (mucosa-associated lymphoid tissue) lymphoma and adenocarci...
Source: Microbiological Research - October 6, 2018 Category: Infectious Diseases Source Type: research

Organization of multi-binding to host proteins: The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of Mycoplasma pneumoniae
Publication date: January 2019Source: Microbiological Research, Volume 218Author(s): Juliane Grimmer, Roger DumkeAbstractMycoplasma pneumoniae is a frequent cause of community-acquired infections of the human respiratory tract. During the evolutionary adaptation of the bacteria to the host, the genome of the pathogen is strongly reduced resulting in the loss of cell wall, limited metabolic pathways and a relatively small number of virulence factors. As interacting with host proteins, surface-exposed proteins with a primary function in cytosol-located processes of metabolism and regulation such as glycolytic enzymes, heat-s...
Source: Microbiological Research - October 5, 2018 Category: Infectious Diseases Source Type: research

Genome sequence and comparative genomics of Rhizobium sp. Td3, a novel plant growth promoting phosphate solubilizing Cajanus cajan symbiont
Publication date: January 2019Source: Microbiological Research, Volume 218Author(s): Bhagya Iyer, Shalini RajkumarAbstractRhizobium sp. Td3 is a Sesbania plant growth promoting, Cajanus cajan nodulating rhizobia. Studying its whole genome was important as it is a potent phosphate solubilizer with constitutive gluconic acid production ability through operation of the periplasmic glucose oxidation pathway even under conditions of catabolite repression. This is in contrast to the other explored phosphate solubilizers. Rhizobial isolates sequenced so far are known to lack components of the direct glucose oxidation pathway and ...
Source: Microbiological Research - October 5, 2018 Category: Infectious Diseases Source Type: research

Cordycepin and Pentostatin Biosynthesis Gene Identified through Transcriptome and Proteomics Analysis of Cordyceps kyushuensis Kob
This study first sequenced transcriptome and proteomics of C. kyushuensis, and demonstrated that there is a single gene cluster related to biosynthesis of cordycepin and pentostatin, which can be employed to improve the yield of cordycepin and find more functional proteins. (Source: Microbiological Research)
Source: Microbiological Research - September 19, 2018 Category: Infectious Diseases Source Type: research

Enhanced molecular visualization of root colonization and growth promotion by Bacillus subtilis EA-CB0575 in different growth systems
In this study, fluorescent in situ hybridization (FISH) and catalyzed reporter deposition–FISH (CARD–FISH) were evaluated to determine the colonization ability of B. subtilis EA-CB0575 on banana and tomato roots planted on solid and liquid Murashige and Skoog medium (MS(S) and MS(L), respectively) and in soil for tomato plants. Results showed B. subtilis colonization 0–30 days post inoculation for banana and tomato plants in different culture systems with differential distribution of bacterial cells along tomato and banana roots. FISH and CARD–FISH methodologies were both successful in detecting B. ...
Source: Microbiological Research - September 13, 2018 Category: Infectious Diseases Source Type: research

The exopolysaccharide gene cluster pea is transcriptionally controlled by RpoS and repressed by AmrZ in Pseudomonas putida KT2440
In this study, we identified that the gene cluster pea was transcribed from 25 bp upstream of the operon and the stationary phase alternative sigma factor RpoS regulated the transcription of pea. When RpoS was absent, another sigma factor, likely the housekeeping sigma factor RpoD, could also mediate pea transcription but at a low level. The function of Pea polysaccharide was further confirmed to be necessary for full production of biofilm, formation of pellicle and c-di-GMP-dependent wrinkly colony morphology. Additionally, evidences were provided to demonstrate that the transcriptional regulator AmrZ was a negative regul...
Source: Microbiological Research - September 11, 2018 Category: Infectious Diseases Source Type: research

Application of laser microdissection to study phytoplasma site-specific gene expression in the model plant Arabidopsis thaliana
In this study, a procedure was established to infect the model plant Arabidopsis thaliana with the phytoplasma Flavescence dorée, a serious threat to European viticulture. Rates of phytoplasma infective insects and transmission efficiency to A. thaliana as well as pathogen loads were measured in different tissues of infected A. thaliana plants, and modification of phloem cell ultrastructure was observed in infected plant tissues at microscopic level. Moreover, a protocol for the application of laser microdissection to analyze plant and phytoplasma gene expression profiles in the specific colonized tissue was designe...
Source: Microbiological Research - September 11, 2018 Category: Infectious Diseases Source Type: research

Blue light exposure and nutrient conditions influence the expression of genes involved in simultaneous hyphal knot formation in Coprinopsis cinerea
Publication date: Available online 8 September 2018Source: Microbiological ResearchAuthor(s): Yuichi Sakamoto, Shiho Sato, Miyuki Ito, Yuki Ando, Kiyoshi Nakahori, Hajime MuraguchiAbstractLight and nutrients are crucial environmental factors influencing fungal sexual reproduction. Blue light induces simultaneous hyphal knot formation in Coprinopsis cinerea mycelia grown on low-glucose media but not in mycelia grown on high-glucose media. Many hyphal knots are visible in the arc near the edge of the colony one day after 15 min of blue light stimulation. These findings collectively suggest that blue light accelerates hypha...
Source: Microbiological Research - September 9, 2018 Category: Infectious Diseases Source Type: research

Isolation, characterization, and selection of heavy metal-resistant and plant growth-promoting endophytic bacteria from root nodules of Robinia pseudoacacia in a Pb/Zn mining area
In this study, we isolated and characterized 82 endophytic bacteria from the root nodules of black locust (Robinia pseudoacacia) grown in a Pb-Zn mining area. There were 80 isolates showing resistance to four HMs, 0.01–18.0 mM/L for Cd, 0.2–40.0 mM/L for Zn, 0.3–2.2 mM/L for Pb, and 0.2–1.4 mM/L for Cu. Indole-3-acetic acid production, siderophore production, and 1-aminocyclopropane-1-carboxylate deaminase activity were detected in 43, 50, and 17 isolates, respectively. Two symbiotic isolates selected with the highest potential for HM resistance and PGP traits, designated...
Source: Microbiological Research - September 7, 2018 Category: Infectious Diseases Source Type: research

Prospects of endophytic fungal entomopathogens as biocontrol and plant growth promoting agents: an insight on how artificial inoculation methods affect endophytic colonization of host plants
Publication date: Available online 31 August 2018Source: Microbiological ResearchAuthor(s): Bamisope Steve Bamisile, Chandra Kanta Dash, Komivi Senyo Akutse, Ravindran Keppanan, Oluwatoyin Grace Afolabi, Mubasher Hussain, Muhammad Qasim, Liande WangAbstractEntomopathogenic fungi (EPF) can be established as endophytes in the host plants to offer a long-term preventive measure for pests and diseases. This practice serves as a better alternative to the common practice of periodic direct application of EPF on plants or the target pests as a short-term defense strategy against pests and diseases. These fungal endophytes, aside ...
Source: Microbiological Research - September 1, 2018 Category: Infectious Diseases Source Type: research

Genome-wide transcriptome response of Streptomyces tsukubaensis to N-acetylglucosamine: effect on tacrolimus biosynthesis
We report here the negative effect of GlcNAc on differentiation and tacrolimus (FK506) production by Streptomyces tsukubaensis NRRL 18488. Using microarrays technology, we found that GlcNAc represses the transcription of fkbN, encoding the main transcriptional activator of the tacrolimus biosynthetic cluster, and of ppt1, encoding a phosphopantheteinyltransferase involved in tacrolimus biosynthesis. On the contrary, GlcNAc stimulated transcription of genes related to amino acid and nucleotide biosynthesis, DNA replication, RNA translation, glycolysis and pyruvate metabolism. The results obtained support those previously re...
Source: Microbiological Research - September 1, 2018 Category: Infectious Diseases Source Type: research

Biological control of the soft rot bacterium Pectobacterium carotovorum by Bacillus amyloliquefaciens strain Ar10 producing glycolipid-like compounds
Publication date: Available online 31 August 2018Source: Microbiological ResearchAuthor(s): Sana Azaiez, Imen Ben Slimene, Ines Karkouch, Rym Essid, Selim Jallouli, Naceur Djebali, Salem Elkahoui, Ferid Limam, Olfa TabbeneAbstractFour hundred and fifty bacteria were evaluated for antagonistic activity against bacterial soft rot of potato caused by Pectobacterium carotovorum sp strain II16. A strain Ar10 exhibiting potent antagonist activity has been identified as Bacillus amyloliquefaciens on the basis of biochemical and molecular characterization. Cell free supernatant showed a broad spectrum of antibacterial activity aga...
Source: Microbiological Research - September 1, 2018 Category: Infectious Diseases Source Type: research

Profiles of quorum sensing (QS)-related sequences in phycospheric microorganisms during a marine dinoflagellate bloom, as determined by a metagenomic approach
Publication date: Available online 30 August 2018Source: Microbiological ResearchAuthor(s): Xinqing Huang, Jianming Zhu, Zhonghua Cai, Yongmin Lao, Hui Jin, Ke Yu, Boya Zhang, Jin ZhouAbstractThe complicated relationships among environmental microorganisms are regulated by quorum sensing (QS). Understanding QS-based signals could shed light on the interactions between microbial communities in certain environments. Although QS characteristics have been widely discussed, few studies have been conducted on the role of QS in phycospheric microorganisms. Here, we used metagenomics to examine the profile of AI-1 (AinS, HdtS, Lux...
Source: Microbiological Research - August 31, 2018 Category: Infectious Diseases Source Type: research

Editorial Board
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): (Source: Microbiological Research)
Source: Microbiological Research - August 30, 2018 Category: Infectious Diseases Source Type: research

Antifungal activity of spider venom-derived peptide lycosin-I against Candida tropicalis
Publication date: Available online 27 August 2018Source: Microbiological ResearchAuthor(s): Li Tan, Le Bai, Ling Wang, Lagu He, Guangdi Li, Wenhan Du, Ting Shen, Zheyi Xiang, Jiali Wu, Zhonghua Liu, Min HuAbstractCandida species are a major cause of human mucosal and deep tissue fungal infections, but few antifungal treatments are available. Here, we showed that lycosin-I, a peptide isolated from venom of the spider Lycosa singoriensis, acted as a potent antifungal inhibitor against Candida species. The MIC50 values of lycosin-I reached 8 µg/mL to treat fluconazole-susceptible and fluconazole-resistant C. tropicali...
Source: Microbiological Research - August 28, 2018 Category: Infectious Diseases Source Type: research

Transcriptional regulation of galF by RcsAB affects capsular polysaccharide formation in Klebsiella pneumoniae NTUH-K2044
In conclusion, RcsAB could regulate the transcription of the galF gene positively by binding to the galF promoter DNA directly, and then affects the CPS formation of K. pneumonia. (Source: Microbiological Research)
Source: Microbiological Research - August 26, 2018 Category: Infectious Diseases Source Type: research

Complete genome sequence of the marine fish pathogen Vibrio anguillarum and genome-wide transposon mutagenesis analysis of genes essential for in vivo infection
This study provides a wealth of information about V. anguillarum genes related to infection in fish and will facilitate the understanding of its pathogenesis as well as the development of diagnostics and vaccines against the pathogen. (Source: Microbiological Research)
Source: Microbiological Research - August 25, 2018 Category: Infectious Diseases Source Type: research

The exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents
Publication date: Available online 23 August 2018Source: Microbiological ResearchAuthor(s): Andrea Santana de Oliveira, Isabella Inês Rodrigues Rosa, Evandro Novaes, Lucas Silva de Oliveira, Lilian Cristiane Baeza, Clayton Luiz Borges, Lennart Marlinghaus, Célia Maria de Almeida Soares, Marcia Giambiagi-deMarval, Juliana Alves Parente-RochaAbstractStaphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins, large-scale proteomic studies have not been perf...
Source: Microbiological Research - August 23, 2018 Category: Infectious Diseases Source Type: research

Dimethyl adenosine transferase (KsgA) contributes to cell-envelope fitness in Salmonella Enteritidis
This study shows that KsgA contributes to cell-envelope fitness and opens new avenues to modulate cell-envelopes via use of KsgA-antagonists. (Source: Microbiological Research)
Source: Microbiological Research - August 23, 2018 Category: Infectious Diseases Source Type: research

Isolation and characterization of actinomycetes from Mural paintings of Snu- Sert-Ankh tomb, their antimicrobial activity, and their biodeterioration
Publication date: Available online 15 August 2018Source: Microbiological ResearchAuthor(s): Abeer F. ElhagrassyAbstractA total of 35 actinomycetes were isolated from the surface of mural paintings of snu- sort- ankh in El Leasht, Egypt during four seasons all over 2016-2017. In 2009 this tomb was deteriorated by "Aspergillus niger, A. flavus, Fusarium moniliforme, Alternaria alternate, Rhizopus stolonifera, Bacillus subtilis, Bacillus cereus, and micrococcus iuteus." In 2017 the isolation of swabs presents only Aspergillus niger and about 35 actinomycetes classified to three different groups "Streptomyces, N...
Source: Microbiological Research - August 15, 2018 Category: Infectious Diseases Source Type: research

Pseudomonas sp. AF-54 containing multiple plant beneficial traits acts as growth enhancer of Helianthus annuus L.under reduced fertilizer input
This study concludes that the Pseudomonas sp. strain AF-54 containing multiple plant growth promoting traits can be a potential candidate for biofertilizer production to enhance sunflower crop yield with reduced application of chemical (NP) fertilizers. (Source: Microbiological Research)
Source: Microbiological Research - August 14, 2018 Category: Infectious Diseases Source Type: research

Emergence of boscalid-resistant strains of Erysiphe necator in French vineyards
In this study, a large-scale survey of French grapevine field populations of E. necator revealed many field populations with low sensitivity to boscalid. Single spore strains originating from collected resistant populations showed Half maximal effective concentration (EC50) values greater than 100 mg L-1, and strains originating from boscalid sensitive populations showed EC50 values lower than 1 mg L-1. The complete nucleotide sequences of the EnSdhB succinate dehydrogenase of sensitive and resistant single spore strains revealed that H242R and H242Y substitutions in the EnSdhB succinate dehydro...
Source: Microbiological Research - August 11, 2018 Category: Infectious Diseases Source Type: research

Biological roles of indole-3-acetic acid in Acinetobacter baumannii
This study further found that A. baumannii also has IAA productive capability, primarily involving the ipdC gene, and transcriptome and spent media analysis of wild-type and mutant cultures grown in minimal media revealed that A. baumannii likely produces IAA through the indole-3-pyruvic acid (IPyA) pathway. Exogenously applied IAA improved tolerance against oxidative stress in wild-type A. baumannii and iacA mutants unable to degrade IAA, but not in ipdC mutants incapable of producing IAA, suggesting that endogenous IAA is important for stress tolerance. Meanwhile, ipdC mutants also had reduced virulence against human A54...
Source: Microbiological Research - August 8, 2018 Category: Infectious Diseases Source Type: research

Growth and protease secretion of Scedosporium aurantiacum under conditions of hypoxia
Publication date: Available online 4 August 2018Source: Microbiological ResearchAuthor(s): Zhiping Han, Liisa Kautto, Wieland Meyer, Sharon C.-A. Chen, Helena NevalainenAbstractOne of the micro-environmental stresses that fungal pathogens, such as Scedosporium aurantiacum, colonising human lungs encounter in vivo is hypoxia, or deficiency of oxygen. In this work, we studied the impacts of a hypoxic micro-environment (oxygen levels ≤1%) on the growth of a clinical S. aurantiacum isolate (WM 06.482; CBS 136046) and an environmental strain (S. aurantiacum WM 10.136; CBS 136049) on mucin-containing synthetic cystic fibrosis...
Source: Microbiological Research - August 5, 2018 Category: Infectious Diseases Source Type: research

Phenotype responses to abiotic stresses, asexual reproduction and virulence among isolates of the entomopathogenic fungus Cordyceps javanica (Hypocreales: Cordycipitaceae)
This study highlights the importance of exploring natural variation in entomopathogenic fungi for selection of appropriate isolates for effective biocontrol of insect pests coupled with mass production characteristics.Graphical abstract (Source: Microbiological Research)
Source: Microbiological Research - August 5, 2018 Category: Infectious Diseases Source Type: research

The first report of antifungal lipopeptide production by a Bacillus subtilis subsp. inaquosorum strain
This study is the first to report the production of two antifungal cyclic lipopeptides (bacillomycin F and fengycin) from a member of B. subtilis subsp. inaquosorum. The strain also produced the antibacterial aurantinin B, which confirms the biosynthetic cluster responsible for its production. Comparative genomics and metabolomics demonstrated the commercial probiotic strain DE111 produced the same metabolites, with the exception of aurantinin B. These findings are the first description of the secondary metabolites produced by a strain of B. subtilis subsp. inaquosorum. (Source: Microbiological Research)
Source: Microbiological Research - August 3, 2018 Category: Infectious Diseases Source Type: research

Soil amendment alters soil physicochemical properties and bacterial community structure of a replanted apple orchard
This study investigated the impact of soil improvement with compost on soil physicochemical properties and bacterial community structure of a replanted apple orchard in comparison with the original orchard without compost improvement. The V1–V3 region of the bacterial 16S rRNA gene was subjected to high-throughput 454 pyrosequencing, and data were analyzed using the Mothur pipeline. The results showed that the soil improvement benefited tree growth and fruit quality during the study period. The compost amendment markedly increased tree height and stem diameter by a range of 6.1% to 21.0% and 4.0% to 14.0%, respective...
Source: Microbiological Research - July 21, 2018 Category: Infectious Diseases Source Type: research

Insights into the carbonic anhydrases and autotrophic carbon dioxide fixation pathways of high CO2 tolerant Rhodovulum viride JA756
Publication date: October 2018Source: Microbiological Research, Volume 215Author(s): Lakshmi Venkata Naga Satya Khandavalli, Tushar Lodha, Maaged Abdullah, Lalitha Guruprasad, Sasikala Chintalapati, Venkata Ramana ChintalapatiAbstractBiofixation of CO2 is being extensively investigated to solve the global warming problem. Purple non-sulfur bacteria are fast growers that consume CO2 and produce beneficial biomass. Better the growth at higher CO2 levels, more efficient are the strains for biofixation. Nine among fifty strains that were analyzed at elevated CO2 levels responded with better growth. Considering its enhanced gro...
Source: Microbiological Research - July 20, 2018 Category: Infectious Diseases Source Type: research

A novel flow-system to establish experimental biofilms for modelling chronic wound infection and testing the efficacy of wound dressings
Publication date: Available online 19 July 2018Source: Microbiological ResearchAuthor(s): Peter F. Duckworth, Richard S. Rowlands, Michele E. Barbour, Sarah E. MaddocksAbstractSeveral models exist for the study of chronic wound infection, but few combine all of the necessary elements to allow high throughput, reproducible biofilm culture with the possibility of applying topical antimicrobial treatments. Furthermore, few take into account the appropriate means of providing nutrients combined with biofilm growth at the air-liquid interface. In this manuscript, a new biofilm flow device for study of wound biofilms is reported...
Source: Microbiological Research - July 20, 2018 Category: Infectious Diseases Source Type: research

Versatile use of Azospirillum brasilense strains tagged with egfp and mCherry genes for the visualization of biofilms associated with wheat roots
This study reports the introduction of egfp or mCherry markers to the Sp245, Sp7, and M40 wild-type strains of Azospirillum brasilense and the hhkB (encoding for a putative hybrid histidine kinase) minus mutant an isogenic strain of A. brasilense Sp245 to monitor colonization of wheat (Triticum aestivum). Two plasmids were constructed: (1) the pJMS-2 suicide plasmid derived from pSUP202 and harboring the mCherry gene expressed under the constitutive kanamycin resistance promoter to create a cis tag and (2) the broad-range plasmid pMP2449-5 that carries the mCherry gene under the lac promoter, which is derived from the plas...
Source: Microbiological Research - July 20, 2018 Category: Infectious Diseases Source Type: research

Paenibacillus lentimorbus induces autophagy for protecting tomato from Sclerotium rolfsii infection
Publication date: Available online 19 July 2018Source: Microbiological ResearchAuthor(s): Ritu Dixit, Lalit Agrawal, Surendra Pratap Singh, Prateeksha, Poonam C. Singh, Vivek Prasad, Puneet Singh ChauhanAbstractDuring biotic stress, plants use several mechanisms to protect themselves that include the production of reactive oxygen species (ROS), induction of pathogenesis-related proteins and cell death. Some plant growth promoting rhizobacteria (PGPR) are known to act as bio-control agents that protect crops against pathogens. The biocontrol activity of PGPR Paenibacillus lentimorbus (B-30488) against Sclerotium rolfsii sho...
Source: Microbiological Research - July 20, 2018 Category: Infectious Diseases Source Type: research