Epigenetics in obesity: Mechanisms and advances in therapies based on natural products
XXXX AbstractObesity is a major risk factor for morbidity and mortality because it has a close relationship to metabolic illnesses, such as diabetes, cardiovascular diseases, and some types of cancer. With no drugs available, the mainstay of obesity management remains lifestyle changes with exercise and dietary modifications. In light of the tremendous disease burden and unmet therapeutics, fresh perspectives on pathophysiology and drug discovery are needed. The development of epigenetics provides a compelling justification for how environmental, lifestyle, and other risk factors contribute to the pathogenesis of obesity. ...
Source: Pharmacology Research and Perspectives - January 31, 2024 Category: Drugs & Pharmacology Authors: Peng Chen,
Yulai Wang,
Fuchao Chen,
Benhong Zhou Tags: REVIEW Source Type: research
Molecules, Vol. 29, Pages 604: Highly Promiscuous Flavonoid Di-O-glycosyltransferases from Carthamus tinctorius L.
This study provides valuable insights into the biosynthetic pathways of flavonoid glycosides in safflower. The discovery of CtOGT1 and CtOGT2 enhances our understanding of the enzymatic processes involved in synthesizing flavonoid glycosides in safflower, contributing to the overall comprehension of secondary metabolite biosynthesis in this plant species. (Source: Molecules)
Source: Molecules - January 26, 2024 Category: Chemistry Authors: Xiaoyu Xu Meng Xia Yang Han Honghu Tan Yanying Chen Xinqi Song Shijun Yuan Yifeng Zhang Ping Su Luqi Huang Tags: Article Source Type: research
Genistein stimulates the viability and prevents myofibroblastic transformation in human trabecular meshwork cells stimulated by TGF- β
Exp Eye Res. 2024 Jan 23;240:109806. doi: 10.1016/j.exer.2024.109806. Online ahead of print.ABSTRACTPrimary open-angle glaucoma (POAG) is the most common type of glaucoma leading to blindness. The search for ways to prevent/treat this entity is one of the main challenges of today's ophthalmology. One of such solution seems to be biologically active substances of natural origin, such as genistein (GEN), which can affect the function of isolated trabecular meshwork by the inhibition of protein tyrosine kinase. However, the role of GEN in viability as well as myofibroblastic transformation in human trabecular meshwork cells s...
Source: Experimental Eye Research - January 25, 2024 Category: Opthalmology Authors: Robert Wasilewicz Julia Wasilewicz Ewa Pruszy ńska-Oszmałek Kinga Stuper-Szablewska Natalia Leciejewska Pawe ł Antoni Kołodziejski Source Type: research
Effect of exogenous genistein on osteogenic differentiation of adipose-derived mesenchymal stem cells in laying hens
Tissue Cell. 2024 Jan 4;87:102299. doi: 10.1016/j.tice.2023.102299. Online ahead of print.ABSTRACTPrevious literature revealed that genistein might play a preventive role in osteoporosis. Therefore, we aimed to evaluate the effect of genistein on the osteogenic potency of laying hens' adipose-derived stem cells (LHASCs). The viability of LHASCs after isolation was investigated on tissue culture plastic (TCP) under exposure to genistein up to 50 μg/mL by MTT assay. Our preliminary result revealed that LHASCs cultured under genistein exposure up to 20 μg/mL are feasible. Then, we evaluated the osteogenic induction of LHASC...
Source: Tissue and Cell - January 16, 2024 Category: Cytology Authors: Farhang Ahmadian Mehrdad Irani Abdollah Mohammadi-Sangcheshmeh Source Type: research
Identification of potential crucial genes and therapeutic targets for epilepsy
ConclusionsThe results of this study highlightPTPRO andGADD45A as potential genes for the diagnosis and treatment of epilepsy. (Source: European Journal of Medical Research)
Source: European Journal of Medical Research - January 11, 2024 Category: Research Source Type: research
Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
This study describes the characterization of the recombinant GH3 aryl-β-glucosidase "GluLm" from Limosilactobacillus mucosae INIA P508, followed by its immobilization on an agarose support with the aim of developing an efficient application to increase the availability and concentration of flavonoid and lignan aglycones in a vegetal beverage. In previous studies, heterologous GluLm-producing strains demonstrated a great capacity to deglycosylate flavonoids. Nevertheless, the physicochemical properties and substrate spectrum of the enzyme remained unknown up to now. A high production of purified GluLm was achieved (14 mg L...
Source: Applied Microbiology and Biotechnology - January 8, 2024 Category: Microbiology Authors: Jos é Antonio Curiel Ana Ruiz de la Bastida Susana Langa Ángela Peirotén Jos é María Landete Source Type: research
Characterization and stabilization of GluLm and its application to deglycosylate dietary flavonoids and lignans
This study describes the characterization of the recombinant GH3 aryl-β-glucosidase "GluLm" from Limosilactobacillus mucosae INIA P508, followed by its immobilization on an agarose support with the aim of developing an efficient application to increase the availability and concentration of flavonoid and lignan aglycones in a vegetal beverage. In previous studies, heterologous GluLm-producing strains demonstrated a great capacity to deglycosylate flavonoids. Nevertheless, the physicochemical properties and substrate spectrum of the enzyme remained unknown up to now. A high production of purified GluLm was achieved (14 mg L...
Source: Applied Microbiology and Biotechnology - January 8, 2024 Category: Microbiology Authors: Jos é Antonio Curiel Ana Ruiz de la Bastida Susana Langa Ángela Peirotén Jos é María Landete Source Type: research
Soy isoflavones induces mitophagy to inhibit the progression of osteosarcoma by blocking the AKT/mTOR signaling pathway
CONCLUSIONS: SI induces mitophagy in OS cells by blocking the AKT/mTOR pathway, contributing to the inhibition of OS.PMID:38191316 | PMC:PMC10775635 | DOI:10.1186/s10020-024-00778-y (Source: Molecular Medicine)
Source: Molecular Medicine - January 8, 2024 Category: Molecular Biology Authors: Ziang Zheng Xinghan Zhao Bo Yuan Shan Jiang Rushan Yan Xiaowei Dong Qijun Yao Haidong Liang Source Type: research
Oxidative stress in Alzheimer's disease: current knowledge of signaling pathways and therapeutics
This study describes in detail the role of oxidative stress in AD, major signaling pathways involving oxidative stress induced AD and drugs under development targeting these pathways which may aid in therapeutic advances for AD.PMID:38165499 | DOI:10.1007/s11033-023-09021-z (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - January 2, 2024 Category: Molecular Biology Authors: Rishika Dhapola Samir K Beura Prajjwal Sharma Sunil K Singh Dibbanti HariKrishnaReddy Source Type: research
Oxidative stress in Alzheimer's disease: current knowledge of signaling pathways and therapeutics
This study describes in detail the role of oxidative stress in AD, major signaling pathways involving oxidative stress induced AD and drugs under development targeting these pathways which may aid in therapeutic advances for AD.PMID:38165499 | DOI:10.1007/s11033-023-09021-z (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - January 2, 2024 Category: Molecular Biology Authors: Rishika Dhapola Samir K Beura Prajjwal Sharma Sunil K Singh Dibbanti HariKrishnaReddy Source Type: research
Methionine gamma lyase: Structure-activity relationships and therapeutic applications
Biochim Biophys Acta Proteins Proteom. 2023 Dec 24:140991. doi: 10.1016/j.bbapap.2023.140991. Online ahead of print.ABSTRACTMethionine gamma lyase (MGL) is a bacterial and plant enzyme that catalyzes the conversion of methionine in methanthiol, 2-oxobutanoate and ammonia. The enzyme belongs to fold type I of the pyridoxal 5'-dependent family. The catalytic mechanism and the structure of wild type MGL and variants were determined in the presence of the natural substrate as well as of many sulfur-containing derivatives. Structure-function relationship studies were pivotal for MGL exploitation in the treatment of cancer, bact...
Source: Biochimica et Biophysica Acta - December 26, 2023 Category: Biochemistry Authors: Samanta Raboni Serena Faggiano Stefano Bettati Andrea Mozzarelli Source Type: research
Enhancement of the organic acid content and antioxidant capacity of yellow whey through fermentation with Lacticaseibacillus casei YQ336
In this study,Lacticaseibacillus casei YQ336 was used to ferment yellow whey, and physical and chemical analysis was performed to identify the changes in the nutritional components and antioxidant activity of the fermented yellow whey. Non-targeted metabolomics was used to study the transformation of small molecular substances in the fermented yellow whey. After 48 h of pure culture fermentation withL. casei YQ336, the pH of yellow whey decreased significantly (p < 0.05). Meanwhile, the content of total acids, organic acids, sugars, total phenols, and total flavonoids and the antioxidant activity showed a signifi...
Source: World Journal of Microbiology and Biotechnology - December 26, 2023 Category: Microbiology Source Type: research
Solid-phase fluorescence: Reproducibility and comparison with the solution states
Talanta. 2023 Dec 18;270:125566. doi: 10.1016/j.talanta.2023.125566. Online ahead of print.ABSTRACTSolid-phase fluorescence excitation-emission matrix (SPF-EEM) spectroscopy has potential for non-extractive, non-destructive, and non-contact analytical measurements of powder and solid-state samples, as well as front-face EEM spectroscopy for suspensions of high optical density. However, as there is no unified measurement method for SPF spectroscopy, comparing samples measured in different research fields is difficult. Therefore, this study designs a cell that can be created by a 3D printer and examines reproducibility on me...
Source: Talanta - December 23, 2023 Category: Chemistry Authors: Yuki Nakaya Ayaka Tomita Hiroshi Yamamura Source Type: research
