Filtered By:
Infectious Disease: Gastroenteritis

This page shows you your search results in order of date. This is page number 6.

Order by Relevance | Date

Total 121 results found since Jan 2013.

Influence of various stressors on the expression of core genes of the small interfering RNA pathway in the oriental fruit fly, Bactrocera dorsalis
Abstract RNA interference (RNAi)‐based technology has emerged as a potential tool for controlling insect pests, however, previous studies found that the efficiency of RNAi in Bactrocera dorsalis was variable. In nature, insects often meet various challenges, such as pathogen infections, extreme temperatures, lack of nutrition and heavy metals. To better understand the association of the stressors with efficiency of RNAi, in the current study we tested the expression of three core genes, dicer2 (Bddcr2), r2d2 (Bdr2d2) and argonaute2 (Bdago2), of the small interfering RNA (siRNA) pathway of B. dorsalis upon various stresso...
Source: Insect Science - May 24, 2016 Category: Biology Authors: Yi‐Fei Xie, Jin‐Zhi Niu, Xuan‐Zhao Jiang, Wen‐Jia Yang, Guang‐Mao Shen, Dong Wei, Guy Smagghe, Jin‐Jun Wang Tags: ORIGINAL ARTICLE Source Type: research

Kaempferol targets Krt-14 and induces cytoskeletal mineralization in osteoblasts: A mechanistic approach
Publication date: Available online 5 March 2016 Source:Life Sciences Author(s): Vikram Khedgikar, Priyanka Kushwaha, Jyoti Gautam, Shewta Sharma, Ashwni Verma, Dharmendra Choudhary, Prabhat R. Mishra, Ritu Trivedi Kaempferol (KEM) has been observed to stimulate Krt-14 protein which subsequently contributes to matrix maturation and mineralization in rat primary osteoblast cells. Incorporation of Krt-14 siRNA results in reduced mRNA and protein expression after 48h post transfection and remained low for 9days. At day 9 Krt-14 siRNA significantly reduced mineralization without concomitant change in the cell number. ...
Source: Life Sciences - March 6, 2016 Category: Biology Source Type: research

Subtilase cytotoxin produced by locus of enterocyte effacement ‐negative Shiga‐toxigenic Escherichia coli induces stress granule formation
Summary Subtilase cytotoxin (SubAB) is mainly produced by locus of enterocyte effacement (LEE)‐negative strains of Shiga‐toxigenic Escherichia coli (STEC). SubAB cleaves an endoplasmic reticulum (ER) chaperone, BiP/Grp78, leading to induction of ER stress. This stress causes activation of ER stress sensor proteins and induction of caspase‐dependent apoptosis. We found that SubAB induces stress granules (SG) in various cells. Aim of this study was to explore the mechanism by which SubAB induced SG formation. Here, we show that SubAB‐induced SG formation is regulated by activation of double‐stranded RNA‐activated...
Source: Cellular Microbiology - February 20, 2016 Category: Microbiology Authors: Hiroyasu Tsutsuki, Kinnosuke Yahiro, Kohei Ogura, Kimitoshi Ichimura, Sunao Iyoda, Makoto Ohnishi, Sayaka Nagasawa, Kazuko Seto, Joel Moss, Masatoshi Noda Tags: Original article Source Type: research

Repression of a chromatin modifier aggravates lipopolysaccharide-induced acute lung injury in mouse.
Abstract Local inflammatory responses and alveolar epithelial cells (AECs) apoptosis are both important for the development of the acute lung injury (ALI), a clinically important complication causing high morbidity and mortality, but little is known about the molecular mechanisms underlying the pathogenesis. Herein, we showed for the first time that expression of Metastasis-associated protein 1 (MTA1), a master transcriptional regulator with the ability to regulate divergent cellular pathways by modifying the acetylation status of crucial target genes, was up-regulated in the alveolar cells of the Escherichia coli...
Source: Biochemical and Biophysical Research communications - February 15, 2016 Category: Biochemistry Authors: Chen JK, Wang WC, Zang L, Zhao J, Li W, Jiang T Tags: Biochem Biophys Res Commun Source Type: research

The Tyrosine Kinase Pyk2 Contributes to Complement-Mediated Phagocytosis in Murine Macrophages
Proline-rich tyrosine kinase 2 (Pyk2) is a member of the focal adhesion kinase (FAK) family and is mainly expressed in neuronal and hematopoietic cells. As FAK family members are involved in signaling connections downstream of integrins, we studied the role of Pyk2 in complement-receptor 3 (CR3, also known as Mac-1, integrin αMβ2, CD11b/CD18)-mediated phagocytosis, a key process in innate immunity. Using 3 independent approaches, we observed that Pyk2 contributes to CR3-dependent phagocytosis by RAW 264.7 macrophages, but is dispensable for Fc#x03B3; receptor (Fc#x03B3;R)-mediated uptake. Reduction of Pyk2 expression lev...
Source: Journal of Innate Immunity - February 6, 2016 Category: Allergy & Immunology Source Type: research

Overexpression of Transmembrane Protein BST2 is Associated with Poor Survival of Patients with Esophageal, Gastric, or Colorectal Cancer.
CONCLUSIONS: The results suggest that BST-2 is involved in tumor progression and serves as an independent prognostic classifier for patients with GC. Because BST-2 is expressed on the cell membrane, BST-2 could be a therapeutic target for GC, CRC, and ESCC. PMID: 26832883 [PubMed - as supplied by publisher]
Source: Ann Oncol - February 1, 2016 Category: Cancer & Oncology Authors: Mukai S, Oue N, Oshima T, Mukai R, Tatsumoto Y, Sakamoto N, Sentani K, Tanabe K, Egi H, Hinoi T, Ohdan H, Yasui W Tags: Ann Surg Oncol Source Type: research

Overexpression of Transmembrane Protein BST2 is Associated with Poor Survival of Patients with Esophageal, Gastric, or Colorectal Cancer
Conclusions The results suggest that BST-2 is involved in tumor progression and serves as an independent prognostic classifier for patients with GC. Because BST-2 is expressed on the cell membrane, BST-2 could be a therapeutic target for GC, CRC, and ESCC.
Source: Annals of Surgical Oncology - February 1, 2016 Category: Cancer & Oncology Source Type: research

Nod-like receptor protein 3 inflammasome activation by Escherichia coli RNA induces transforming growth factor beta 1 secretion in hepatic stellate cells.
Authors: Wang H, Liu S, Wang Y, Chang B, Wang B Abstract Nod-like receptor protein 3 (NLRP3) inflammasome has been implicated in alcoholic liver disease. Chronic alcohol consumption enhances gut permeability and causes microbial translocation. The present study explored the activation of the NLRP3 inflammasome by Escherichia coli RNA in hepatic stellate cells (HSCs), and the potential role of NLRP3 inflammasome in hepatic fibrosis. E. coli RNA transfection induced HSC-T6 cells to secrete and express mature interleukin-1 beta (IL-1β), which was abolished by NLRP3 siRNA pretreatment. In addition, E. coli RNA transfe...
Source: Bosnian Journal of Basic Medical Sciences - January 20, 2016 Category: Journals (General) Tags: Bosn J Basic Med Sci Source Type: research

Subtilase cytotoxin produced by locus of enterocyte effacement‐negative shiga‐toxigenic Escherichia coli induces stress granule formation
This article is protected by copyright. All rights reserved.
Source: Cellular Microbiology - January 9, 2016 Category: Microbiology Authors: Hiroyasu Tsutsuki, Kinnosuke Yahiro, Kohei Ogura, Kimitoshi Ichimura, Sunao Iyoda, Makoto Ohnishi, Sayaka Nagasawa, Kazuko Seto, Joel Moss, Masatoshi Noda Tags: Research article Source Type: research

Abstract C171: Human anti-Nucleolin recombinant immunoagents as new potential tools for melanoma treatment
Immunotherapy and immune-based anti-cancer molecules represent a valid strategy to fight cancer. However, the choice of tumor-specific surface molecules for the selective targeting of cancer cells still represents a critical step in the study design for the development of new therapeutic approaches. Notably, the development of phage-display technology for the selection of fully human single chain antibody fragments (scFvs) and complete antibodies directed toward tumor-associated antigens has represented a significant advancement for immunotherapy.Nucleolin (NCL) is one of the most abundant non-ribosomal proteins in the nuc...
Source: Molecular Cancer Therapeutics - January 7, 2016 Category: Cancer & Oncology Authors: Braddom, A., Richmond, T., Sheetz, T., Reese, E., Tessari, A., Tober, K., Burd, C. E., De Lorenzo, C., Martin, E. W., Coppola, V., Tweedle, M. F., Oberyszyn, T., Croce, C. M., Palmieri, D. Tags: Therapeutic Agents: Biological: Poster Presentations - Proffered Abstracts Source Type: research

Influence of various stressors to the expression of core genes of the small interfering RNA pathway in the oriental fruit fly, Bactrocera dorsalis (Hendel)
This article is protected by copyright. All rights reserved
Source: Insect Science - January 6, 2016 Category: Biology Authors: Yi‐Fei Xie, Jin‐Zhi Niu, Xuan‐Zhao Jiang, Wen‐Jia Yang, Guang‐Mao Shen, Dong Wei, Guy Smagghe, Jin‐Jun Wang Tags: ORIGINAL ARTICLE Source Type: research

SIGIRR participates in negative regulation of LPS response and tolerance in human bladder epithelial cells
Conclusions: In conclusion, our results suggest that SIGIRR plays an important role in the negative regulation of LPS response and tolerance in human bladder epithelial cells, possibly through its impact on TLR-mediated signaling.
Source: BMC Immunology - December 3, 2015 Category: Allergy & Immunology Authors: Dan LiXin ZhangBaiyi Chen Source Type: research

Mycoplasma gallisepticum (HS strain) surface lipoprotein pMGA interacts with host apolipoprotein A-I during infection in chicken.
In this study, we prepared the membrane fraction of trachea epithelial cells from chicken embryos. Using an improved virus overlay protein blot assay (VOPBA) and glutathione S-transferase (GST) pull-down assay, we found that pMGA1.2 specifically bound to a ∼30 kDa host protein. This host protein was further identified by mass spectrometry as chicken apolipoprotein A-I (ApoA-I). We expressed and purified the recombinant ApoA-I protein in Escherichia coli and confirmed that it bound to the purified pMGA1.2 protein in vitro. Transiently expressed pMGA1.2 and ApoA-I were colocalized in HeLa cells. Finally, we designed small...
Source: Applied Microbiology and Biotechnology - November 9, 2015 Category: Microbiology Authors: Hu F, Zhao C, Bi D, Tian W, Chen J, Sun J, Peng X Tags: Appl Microbiol Biotechnol Source Type: research

APC functions at the centrosome to stimulate microtubule growth.
Abstract The adenomatous polyposis coli (APC) tumor suppressor is multi-functional. APC is known to localize at the centrosome, and in mitotic cells contributes to formation of the mitotic spindle. To test whether APC contributes to nascent microtubule (MT) growth at interphase centrosomes, we employed MT regrowth assays in U2OS cells to measure MT assembly before and after nocodazole treatment and release. We showed that siRNA knockdown of full-length APC delayed both initial MT aster formation and MT elongation/regrowth. In contrast, APC-mutant SW480 cancer cells displayed a defect in MT regrowth that was unaffe...
Source: The International Journal of Biochemistry and Cell Biology - November 7, 2015 Category: Biochemistry Authors: Lui C, Ashton C, Sharma M, Brocardo MG, Henderson BR Tags: Int J Biochem Cell Biol Source Type: research

LSC Abstract - Toll-like receptor 10: Expression and functional role in LPS mediated inflammation
We examined TLR10 expression in normal and inflamed lungs from chickens and humans. Immunohistochemistry showed TLR10 in vascular endothelium in human and chicken lungs. Immunohistochemistry and Western blots showed an increase in TLR10 protein in lungs of chicken infected with E. coli or Fowl Adenovirus. Human neutrophils challenged with E. coli lipopolysaccharide (LPS) showed decreased total TLR10 protein and surface expression in 90 minutes. Confocal microscopy showed cytosolic and nuclear distribution of TLR10 in normal neutrophils. In the LPS-activated neutrophils, TLR10 colocalized with flotallin-1, a lipid raft mark...
Source: European Respiratory Journal - October 30, 2015 Category: Respiratory Medicine Authors: Balachandran, Y., Singh, B. Tags: 3.1 Molecular Pathology and Functional Genomics Source Type: research