Inflammatory cells in perivascular adipose tissue and the integrity of the tunica media in atherosclerotic coronary arteries.
We examined the relationship between inflammatory infiltration in perivascular adipose tissue (PVAT), state of the external elastic membrane, and the intensity of inflammatory infiltration in the tunica media of coronary arteries obtained by endarterectomy from symptomatic patients with diffuse CAD. We analyzed endarterectomy sequesters from 22 coronary arteries that contained the intima, media, a part of the adventitia, and PVAT in at least one part of the sequester. The coronary arteries were divided into two groups according to the presence or absence of inflammatory infiltration in PVAT. Staining with hematoxylin-eosin...
Source: Bosnian Journal of Basic Medical Sciences - September 30, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Mesenchymal stem cells from bone marrow regulate invasion and drug resistance of multiple myeloma cells by secreting chemokine CXCL13.
This study provides valuable experimental evidence for clinical MM therapy. PMID: 31538911 [PubMed - as supplied by publisher] (Source: Bosnian Journal of Basic Medical Sciences)
Source: Bosnian Journal of Basic Medical Sciences - September 22, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

A male-specific association between AGTR1 hypermethylation and coronary heart disease.
Authors: Li X, Wu N, Ji H, Huang Y, Hu H, Li J, Mi S, Duan S, Chen X Abstract The AGTR1 gene encodes angiotensin II receptor type 1, which is involved in cardiovascular diseases such as coronary heart disease (CHD). In the current study, we analyzed AGTR1 methylation level in a Han Chinese population by SYBR green-based quantitative methylation-specific PCR (qMSP). We collected blood samples from 761 CHD patients and 398 non-CHD controls at the Ningbo First Hospital. A data mining analysis was also performed to explore the association between AGTR1 methylation and AGTR1 gene expression, using datasets from the cBio...
Source: Bosnian Journal of Basic Medical Sciences - September 22, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

The use of cellular matrix in symptomatic knee osteoarthritis.
Authors: Abbassy AA, Trebinjac S, Kotb N Abstract Knee osteoarthritis is a degenerative "wear and tear" disorder affecting mainly population over 50 years old. It can also present in younger people, especially after an injury or as a part of other diseases. While many therapeutic options exist for knee osteoarthritis, none of them has the potential to cure this condition. Cellular Matrix represents a combination of natural non-crosslinked hyaluronic acid (HA), thixotropic cell separation gel, and sodium citrate anticoagulant solution. A combination of Cellular Matrix with autologous platelet-rich plasma (...
Source: Bosnian Journal of Basic Medical Sciences - September 22, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

HPV infection screening using surface plasmon resonance in women from Kunming (southwest China).
Authors: Liu Y, Quan Y, Xu C, Huang Y, Li D, Qing Q, Sun C, Zhou H Abstract No study examined the frequency of human papillomavirus (HPV) genotypes by surface plasmon resonance (SPR) in southwest China. This was a cross-sectional survey (The Second Affiliated Hospital of Kunming Medical University, 10/2010 to 12/2011) in 150 patients who were hospitalized or volunteered for cervical cancer (CC) screening. A HPV typing kit was used to detect 24 types of HPV by the SPR technique. The HPV-positive rate was 34.8% in women with normal cytology and 92.9% in women with CC. The frequency of HPV16 increased from 9.4% for wo...
Source: Bosnian Journal of Basic Medical Sciences - September 13, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

The effects of exercise on vascular markers and C-reactive protein among obese children and adolescents: An evidence-based review.
Authors: Salamt N, Muhajir M, Aminuddin A, Ugusman A Abstract Numerous studies have evaluated the effects of exercise training on obese children and adolescents. However, the impact of aerobic and/or resistance exercise alone, without any other interventions, on vascular markers and C-reactive protein (CRP) in obese children and adolescents is still not clear. We performed a literature search in Ovid Medline, PubMed, and SCOPUS databases to identify articles on the effects of exercise on vascular markers and CRP among obese children and adolescents, published between January 2009 and May 2019. Only full-text articl...
Source: Bosnian Journal of Basic Medical Sciences - September 13, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

A preliminary study of microRNA expression in different types of primary melanoma.
Authors: Gencia I, Baderca F, Avram S, Gogulescu A, Marcu A, Seclaman E, Marian C, Solovan C Abstract MicroRNAs (miRNAs) have been proven to regulate the development and progression of cancer through various mechanisms. The aim of the present study was to compare miRNA expression between primary melanomas from different sites. We analyzed the expression of 84 miRNAs in 27 primary melanoma and 5 nevus formalin-fixed paraffin-embedded (FFPE) samples using the Human Cancer PathwayFinder miScript miRNA PCR Array. The FFPE samples were obtained from the archives of the Municipal Clinical Emergency Hospital of Timisoara ...
Source: Bosnian Journal of Basic Medical Sciences - September 5, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Long noncoding RNA MALAT1 may be a prognostic biomarker in IDH1/2 wild-type primary glioblastomas.
Authors: Argadal OG, Mutlu M, Ak Aksoy S, Kocaeli H, Tunca B, Civan MN, Egeli U, Cecener G, Bekar A, Taskapilioglu MO, Tekin C, Tezcan G, Tolunay S Abstract Primary glioblastoma (GB) is the most aggressive type of brain tumors. While mutations in isocitrate dehydrogenase (IDH) genes are frequent in secondary GBs and correlate with a better prognosis, most primary GBs are IDH wild-type. Recent studies have shown that the long noncoding RNA metastasis associated lung adenocarcinoma transcript-1 (MALAT1) is associated with aggressive tumor phenotypes in different cancers. Our aim was to clarify the prognostic signific...
Source: Bosnian Journal of Basic Medical Sciences - September 5, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Tissue-based metabolomics reveals potential biomarkers for cervical carcinoma and HPV infection.
Authors: Abudula A, Rouzi N, Xu L, Yang Y, Hasimu A Abstract Aberrant metabolic regulation has been observed in human cancers, but the corresponding regulation in human papillomavirus (HPV) infection-associated cervical cancer is not well understood. Here, we explored potential biomarkers for the early prediction of cervical carcinoma based on the metabolic profile of uterine cervical tissue specimens that were positive for HPV16 infection. Fifty-two fresh cervical tissues were collected from women confirmed to have cervical squamous cell carcinoma (SCC; n = 21) or cervical intraepithelial neoplasia (CIN) stages II...
Source: Bosnian Journal of Basic Medical Sciences - August 31, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Effect of SOD2 methylation on mitochondrial DNA4834-bp deletion mutation in marginal cells under oxidative stress.
Authors: Li J, Dai X, He X, Yang R, Xia Z, Xiao H Abstract Presbycusis, or age-related hearing loss, is a prevalent disease that severely affects the physical and mental health of the elderly. Oxidative stress and mitochondrial (mt)DNA deletion mutation are considered as major factors in the pathophysiology of age-related hearing loss. The 4977-bp deletion in human mtDNA (common deletion, corresponding to the 4834-bp mtDNA deletion in rats) is suggested to be closely associated with the pathogenesis of age-related hearing loss. Superoxide dismutase 2 (SOD2), an isoform of SOD that is exclusively expressed in the in...
Source: Bosnian Journal of Basic Medical Sciences - August 31, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Pathogenesis of atherosclerosis in the tunica intima, media, and adventitia of coronary arteries: An updated review.
Authors: Milutinović A, Šuput D, Zorc-Pleskovič R Abstract Atherosclerosis is a chronic inflammatory disease of arteries and it affects the structure and function of all three layers of the coronary artery wall. Current theories suggest that the dysfunction of endothelial cells is one of the initial steps in the development of atherosclerosis. The view that the tunica intima normally consists of a single layer of endothelial cells attached to the subendothelial layer and internal elastic membrane has been questioned in recent years. The structure of intima changes with age and it becomes multilayered due t...
Source: Bosnian Journal of Basic Medical Sciences - August 31, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Comparison of the effects of preoperative melatonin or vitamin C administration on postoperative analgesia.
Authors: Laflı Tunay D, Türkeün Ilgınel M, Ünlügenç H, Tunay M, Karacaer F, Biricik E Abstract The analgesic benefit of melatonin and vitamin C as primary or adjuvant agents has been reported in various studies; however, their analgesiceffects in the treatment of postoperative pain remain unclear. Thus, we aimed to evaluate the effect of preoperative single dose of oral melatonin and vitamin C administration on postoperative analgesia. One hundred sixty-five adult patients undergoing elective major abdominal surgery with general anesthesia were recruited in this study. Patients were ran...
Source: Bosnian Journal of Basic Medical Sciences - August 31, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Making sense of subclinical cardiac alterations in patients with diabetes.
Authors: Pareek M, Olsen MH Abstract PMID: 31394053 [PubMed - as supplied by publisher] (Source: Bosnian Journal of Basic Medical Sciences)
Source: Bosnian Journal of Basic Medical Sciences - August 10, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

The potential of metabolic and lipid profiling in inflammatory bowel diseases: a pilot study.
Authors: Tefas C, Ciobanu L, Tanțău M, Moraru C, Socaciu C Abstract Inflammatory bowel diseases (IBDs) are conditions that still pose significant problems. A third of the patients are either misdiagnosed or a proper diagnosis of Crohn's disease (CD) or ulcerative colitis (UC) cannot be made. We need new biomarkers, so that we can offer patients the best treatment and keep the disease in an inactive state for as long as possible. Alterations in metabolic profiles have been incriminated in the pathophysiology of IBD. The aim of the present study was to identify molecules that could serve as biomarkers for a positiv...
Source: Bosnian Journal of Basic Medical Sciences - August 3, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

A novel in ovo model to study cancer metastasis using chicken embryos and GFP expressing cancer cells.
In conclusion, our investigation provides a new cancer metastasis model that can be further improved to include more complex aspects, such as the use of multiple cell lines and anti-metastatic agents, thus opening new horizons in cancer biology and pharmaceutical research. PMID: 31336058 [PubMed - as supplied by publisher] (Source: Bosnian Journal of Basic Medical Sciences)
Source: Bosnian Journal of Basic Medical Sciences - July 25, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Effect of CCT137690 on long non-coding RNA expression profiles in MCF-7 and MDA-MB-231 cell lines.
Authors: Balcı Okcanoğlu T, Kayabaşı Ç, Gündüz C Abstract Long non-coding RNAs (lncRNAs) are involved in a range of biological processes, such as cellular differentiation, migration, apoptosis, invasion, proliferation, and transcriptional regulation. The aberrant expression of lncRNAs plays a significant role in several cancer types. Aurora kinases are increasingly expressed in various malignancies; accordingly, the inhibition of these enzymes may represent a novel approach for the treatment of various cancers. CCT137690, an Aurora kinase inhibitor, displays an anti-proliferative activity in hu...
Source: Bosnian Journal of Basic Medical Sciences - July 20, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Postoperative pulmonary complications in contemporary cohort of patients with pulmonary hypertension.
Authors: Kruthiventi SC, Kane GC, Sprung J, Weingarten TN, Warner ME Abstract Patients with pulmonary hypertension are at increased risk for postoperative pulmonary complications (PPCs). Herein, we review PPCs in pulmonary hypertension patients undergoing non-cardiac procedures under general anesthesia. The medical records of pulmonary hypertension patients who underwent surgery with general anesthesia between 2010 and 2017 were reviewed for PPCs. In addition we reviewed nursing-documented respiratory depressive episodes in the post-anesthesia care unit to assess the associations between these episodes and later PP...
Source: Bosnian Journal of Basic Medical Sciences - July 15, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Integrin beta 4 (ITGB4) and its tyrosine-1510 phosphorylation promote pancreatic tumorigenesis and regulate the MEK1-ERK1/2 signaling pathway.
In this study we investigated the role of integrin b4 (ITGB4) and its phosphorylation at tyrosine Y1510 (p-ITGB4-Y1510) in the tumorigenesis of pancreatic cancer. We analyzed the expression of ITGB4 and p-ITGB4-Y1510 in pancreatic cancer tissue and cell lines using immunohistochemistry, Western blot, or semi-quantitative reverse transcription PCR. ITGB4 and p-ITGB4-Y1510 were highly expressed in pancreatic cancer (n = 176) compared with normal pancreatic tissue (n = 171). High p-ITGB4-Y1510 expression correlated with local invasion and distant metastasis of pancreatic cancer, and high ITGB4 was significantly associated wit...
Source: Bosnian Journal of Basic Medical Sciences - June 28, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Interleukins and inflammatory markers are useful in predicting the severity of acute pancreatitis.
Authors: Ćeranić DB, Zorman M, Skok P Abstract Acute pancreatitis (AP) is a disease with significant morbidity and mortality. The aim of this study was to evaluate the prognostic role of inflammatory markers, particularly interleukins (ILs), in the course of AP and to determine the frequency of etiologic factors of AP. We included patients with AP who were treated at our institution from May 1, 2012 to January 31, 2015. Different laboratory parameters, including ILs, and the severity scoring systems Ranson's criteria and Bedside Index of Severity in Acute Pancreatitis (BISAP) were analyzed. AP was classified into...
Source: Bosnian Journal of Basic Medical Sciences - June 28, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Predictors of early cardiac changes in patients with type 1 diabetes mellitus: an echocardiography-based study.
Authors: Šuran D, Kanič V, Naji F, Krajnc I, Čokolič M, Zemljič E, Sinkovič A Abstract In patients with type 1 diabetes mellitus (T1DM) imaging studies have demonstrated an increased prevalence of left ventricular diastolic dysfunction and increased left ventricular mass (LVM) unrelated to arterial hypertension and ischemic heart disease. The aim of our study was to identify potential predictors of early subclinical changes in cardiac chamber size and function in such patients. Sixty-one middle-aged asymptomatic normotensive patients with T1DM were included in the study. Conventional and tissue Doppler ...
Source: Bosnian Journal of Basic Medical Sciences - June 21, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Ketamine exerts a protective role in a cell-based model of major depressive disorder via the inhibition of apoptosis and inflammation and activation of the Krebs cycle.
In this study, we established a model of depression in PC12 cells using corticosterone to investigate the effect of ketamine and nuclear factor-κB (NF-κB) on the cell viability, apoptosis, levels of pro-inflammatory cytokines, apoptosis-related molecules, and enzymes of the Krebs cycle. PC12 cells were divided into control (no treatment, NC), ketamine treatment (KT), ketamine treatment with the inhibition of NF-κB (KI), and ketamine treatment with the overexpression of NF-κB (KO) group. Blood serum samples were collected from patients with MDD (n = 10) and healthy controls (n = 10) between 2015 and ...
Source: Bosnian Journal of Basic Medical Sciences - June 21, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Role of FBXW7 in the quiescence of gefitinib-resistant lung cancer stem cells in EGFR-mutant non-small cell lung cancer.
In this study, we developed GRPs from the two NSCLC cell lines PC9 and HCC827, which express an EGFR exon 19 deletion mutation, by treatment with a high concentration of gefitinib. The GRPs from both PC9 and HCC827 cells expressed high levels of CD133 and FBXW7, but low levels of c-MYC. Cell cycle analysis demonstrated that the majority of GRPs existed in the G0/G1 phase. Knockdown of the FBXW7 gene significantly reduced the cell number of CD133-positive GRPs and reversed the cell population in the G0/G1-phase. We also found that FBXW7 expression in CD133-positive cells was increased and c-MYC expression was decreased in g...
Source: Bosnian Journal of Basic Medical Sciences - June 18, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Characteristics and prognostic factors of age-stratified high-grade intracranial glioma patients: A population-based analysis.
Authors: Sun Y, Xiong ZY, Yan PF, Jiang LL, Nie CS, Wang X Abstract We evaluated characteristics and different prognostic factors for survival in age-stratified high-grade glioma in a US cohort. Eligible patients were identified in the Surveillance, Epidemiology, and End Results (SEER) registries and stratified into 3 age groups: 20-39 years old (1,043 patients), 40-59 years old (4,503 patients), and>60 years old (5,045 patients). Overall and cancer-related survival data were obtained. Cox models were built to analyze the outcomes and risk factors. It showed that race was a prognostic factor for survival in pati...
Source: Bosnian Journal of Basic Medical Sciences - June 18, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

PCAT1 is a poor prognostic factor in endometrial carcinoma and associated with cancer cell proliferation, migration and invasion.
In this study, we used 89 EC tissues and HEC-1B, Ishikawa, RL95-2 and AN3CA EC cell lines. We found elevated expression levels of PCAT1 in EC tissues and cell lines using reverse transcription qPCR (RT-qPCR). The prognostic value of PCAT1 was determined using Kaplan-Meier survival and Cox regression analysis. The results showed that higher PCAT1 expression was positively correlated with FIGO stage, myometrial invasion, lymph node metastasis, and a shorter overall survival. A series of functional assays showed that the knockdown of PCAT1 by small interfering RNA (siRNA) targeting PCAT1 (siPCAT1) suppressed cell proliferatio...
Source: Bosnian Journal of Basic Medical Sciences - May 29, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Association of serum chemerin and inflammatory factors with type 2 diabetes macroangiopathy and waist-to-stature ratio.
This study explored the association of chemerin and inflammatory factors with body fat parameters, glucolipid metabolism, and insulin resistance (IR) in T2DM and T2DM-V. Patients were classified into normal glucose regulation (NGR), T2DM, and T2DM-V groups. Serum chemerin, glucolipid metabolic parameters, transforming growth factor (TGF)-β, interleukin (IL)-6, monocyte chemoattractant protein (MCP)-1 and fasting insulin levels were measured along with HOMA-IR, body mass index (BMI), and waist-to-stature ratio (WSR). Serum chemerin, TGF-β, IL-6 and MCP-1 levels were significantly higher in T2DM groups than in NGR ...
Source: Bosnian Journal of Basic Medical Sciences - May 14, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Effects of TCF7L2 rs7903146 variant on metformin response in patients with type 2 diabetes.
In this study we explored the effects of TCF7L2 rs7903146 genotype on metformin response in T2D. The study included 86 newly diagnosed patients with T2D, incident users of metformin. Levels of fasting glucose, insulin, HbA1c, total cholesterol, HDL-cholesterol, LDL-cholesterol, triglycerides, and anthropometric parameters were measured prior to metformin therapy, and 6 and 12 months after the treatment. Genotyping of TCF7L2 rs7903146 was performed by the Sequenom MassARRAY® iPLEX® platform. At baseline, the diabetes risk allele (T) showed an association with lower triglyceride levels (p = 0.037). After 12 months of...
Source: Bosnian Journal of Basic Medical Sciences - May 11, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

The 2019 measles epidemic in Bosnia and Herzegovina: What is wrong with the mandatory vaccination program?
Authors: Arapović J, Sulaver Ž, Rajič B, Pilav A Abstract Measles are a highly contagious and communicable viral disease which may be prevented by a sustained vaccination program. Due to missed vaccination, two major epidemics of measles (1997–1999 and 2014–2015) have been recorded after the war in Bosnia and Herzegovina (BH) with over 10,000 patients registered. According to the World Health Organization, BH is categorized as a country with endemic transmission of measles. The last measles epidemic was between 2014 and 2015, with 5,083 documented patients in the Federation of BH. In the first four m...
Source: Bosnian Journal of Basic Medical Sciences - May 9, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

EP1 receptor is involved in prostaglandin E2-induced osteosarcoma growth.
Authors: Niu JC, Ma N, Liu W, Wang PJ Abstract Recent studies showed that the activation of prostaglandin (PG) receptor EP1 promotes cell migration and invasion in different cancers. The aim of this study was to investigate the role of EP1 in the proliferation of osteosarcoma (OS) cells in vitro and in vivo. EP1 mRNA and protein levels were analyzed by real-time RT-PCR and Western blot, respectively in human OS cell lines MG63, OS732, U-2OS, HOS and SAOS-2 compared to human fetal osteoblastic hFOB 1.19 cells. MG63 cells were treated with PGE2, EP1 specific agonist 17-PT-PGE2, 17-PT-PGE2 + EP1 specific antagonist SC...
Source: Bosnian Journal of Basic Medical Sciences - April 19, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Age- and gender-independent association of glutathione S-transferase null polymorphisms with chronic myeloid leukemia.
Authors: Muddathir ARM, Abdallah EI, Khabour OF, Abdelgader RE, Elgari MM Abstract The glutathione S-transferase (GST) genes encode enzymes that mediate the detoxification of xenobiotics by catalyzing the conjugation of glutathione (GSH) to xenobiotic substrates. The aim of the current study is to investigate the association between GSTT1 and GSTM1 polymorphisms and chronic myeloid leukemia (CML) among Sudanese patients. Patients with CML (n = 115) were recruited to the study from the Radiation and Isotope Centre Khartoum (RICK)-Sudan. Healthy individuals (n = 104) were included as controls. Genotyping of GSTT1 and...
Source: Bosnian Journal of Basic Medical Sciences - April 19, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Optogenetics: Therapeutic spark in neuropathic pain.
Authors: Wang L, Liu K Abstract Optogenetics is an emerging field, which uses light and molecular genetics to manipulate the activity of live cells by expressing light-sensitive proteins. With the discovery of bacteriorhodopsin, a light-sensitive bacterial protein, in 1971 Oesterhelt and Stoeckenius laid the pavement of optogenetics. However, the cross-integration of different disciplines is a little more than a decade old. The toolbox contains fluorescent sensors and optogenetic actuators which enable visualization of signaling events and manipulation of cellular activities, respectively. Neuropathic pain is a pai...
Source: Bosnian Journal of Basic Medical Sciences - April 19, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Expression patterns and prognostic value of miR-210, miR-494, and miR-205 in middle-aged and old patients with sepsis-induced acute kidney injury.
Authors: Lin Y, Ding Y, Song S, Li M, Wang T, Guo F Abstract Septic patients suffer a 'cytokine storm' from proinflammatory cytokines, chemokines and other inflammatory mediators, resulting in acute kidney injury (AKI) and death. The purpose of the present study was to determine the expression patterns of microRNA-210 (miR-210), miR-494, and miR-205 in middle-aged and old patients with sepsis-induced AKI and to evaluate their association with patient prognosis. Serum blood urea nitrogen (BUN), creatinine (Cr) and cystatin C levels were determined in peripheral venous blood collected from 110 patients with sepsis-in...
Source: Bosnian Journal of Basic Medical Sciences - April 19, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

An exceptional group of non-small cell lung cancer difficult to diagnose: Evaluation of lipid-poor adrenal lesions.
Authors: Simsek FS, Arslan M, Dag Y Abstract In some non-small cell lung cancer (NSCLC) patients, lipid-poor adrenal adenomas cannot be adequately differentiated from metastases using imaging methods. Invasive diagnostic procedures also have a low negative predictive value (NPV) in such cases. The current study aims to establish a specific and clinically practical metabolic parameter for lipid-poor adrenal lesions (ALs) in NSCLC patients. This diagnostic approach may prevent unnecessary abdominal enhanced computed tomography (CT), magnetic resonance imaging, or invasive diagnostic procedures. Sixty-four NSCLC patie...
Source: Bosnian Journal of Basic Medical Sciences - April 19, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Clear cell urothelial carcinoma of the urinary bladder - a rare pathological entity. A case report and a systematic review of the literature.
In this study, we report a case of an 81-year-old male, presenting with gross hematuria and acute urinary retention, which was subsequently diagnosed with CCUC at our pathology department. Furthermore, we provide a short systematic review of the literature (PubMed, Scopus, Science Citation Index) for this rare histopathological entity and a brief discussion about its morphological and immunohistochemical (IHC) characteristics. PMID: 30957722 [PubMed - as supplied by publisher] (Source: Bosnian Journal of Basic Medical Sciences)
Source: Bosnian Journal of Basic Medical Sciences - April 9, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Internodal HER2 heterogeneity of axillary lymph node metastases in breast cancer patients.
Authors: Baroš IV, Tanasković N, Pellas U, Eri Ž, Tadić Latinović L, Tot T Abstract Determination of human epidermal growth factor receptor 2 (HER2) status is important for adequate treatment of breast cancer (BC) patients. The novel HER2 gene protein assay (GPA) is particularly convenient, as it allows the simultaneous assessment of HER2 protein expression and gene amplification at individual cell level. Here we investigated the frequency of internodal HER2 heterogeneity in axillary lymph node macrometastases of BC patients and compared HER2 status between primary breast tumor and its metastases. We inc...
Source: Bosnian Journal of Basic Medical Sciences - April 9, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Valspodar-modulated chemotherapy in human ovarian cancer cells SK-OV-3 and MDAH-2774.
In this study, we evaluated the effect of valspodar and cisplatin co-treatment on cell viability, cell death and oxidative status in ovarian cancer cells. The two cisplatin-resistant human ovarian cancer cell lines SK-OV-3 and MDAH-2774 were treated with cisplatin, valspodar, or cisplatin+valspodar for 24 and 48 hours. Untreated cells were used as control group. Cell viability was evaluated by MTT assay. Cell death was assessed by TUNEL and comet assay. Lipid peroxidation (malondialdehyde) and protein thiol groups were analyzed as oxidative stress markers. The expression of mitochondrial superoxide dismutase (MnSOD) was as...
Source: Bosnian Journal of Basic Medical Sciences - April 9, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Targeted immunotherapy with a checkpoint inhibitor in combination with chemotherapy: A new clinical paradigm in the treatment of triple-negative breast cancer.
Authors: Cyprian FS, Akhtar S, Gatalica Z, Vranic S Abstract The treatment of several solid and hematologic malignancies with immune checkpoint inhibitors (against PD-1/PD-L1) has dramatically changed the cancer treatment paradigm. However, no checkpoint inhibitors were previously approved for the treatment of triple-negative breast cancer (TNBC), a difficult-to-treat disease with a high unmet therapeutic need. Based on IMpassion130 clinical trial (NCT02425891), FDA has recently granted an accelerated approval for atezolizumab (TECENTRIQ®), a monoclonal antibody drug targeting PD-L1, plus chemotherapy (Abraxane...
Source: Bosnian Journal of Basic Medical Sciences - March 29, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Recombinant deoxyribonucleoside kinase from Drosophila melanogaster can improve gemcitabine based combined gene/chemotherapy for targeting cancer cells.
This study was designed to evaluate the effect of DmdNKΔC20 for sensitizing human cancer cell lines towards gemcitabine and to further investigate its role in reversal of acquired drug resistance in gemcitabine-resistant cancer cell line. The DmdNKΔC20 gene was delivered to three different cancer cell lines, including breast, colon and liver cancer cells, using lipid-mediated transfection reagent. After transfection, gene expression of DmdNKΔC20 was confirmed by reverse transcription quantitative PCR (qRT-PCR) and the combined effect of DmdNKΔC20 and gemcitabine based cytotoxicity was observed by ce...
Source: Bosnian Journal of Basic Medical Sciences - March 26, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Histological and biochemical effects of Cinnamomum cassia nanoparticles in kidneys of diabetic Sprague-Dawley rats.
This study investigated the antidiabetic activity of Cinnamomum cassia (C. cassia, Cc) silver nanoparticles (CcAgNPS) and effects of C. cassia on the kidneys of rats with induced type 2 diabetes. Twenty-four Sprague-Dawley rats weighing 250 ± 20 g were induced with diabetes by intraperitoneal injection of streptozotocin (STZ, 60 mg/kg). Animals were randomly assigned to one of four groups (n = 6) and treated for eight weeks with normal saline (control, group A), 5 mg/kg of CcAgNPs (group B), 10 mg/kg of CcAgNPs (group C), or 200 mg/kg of Cc (group D). Body weight and fasting blood glucose (FBG) was measured weekly a...
Source: Bosnian Journal of Basic Medical Sciences - March 26, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Epidemiology and mechanism of drug resistance of Mycoplasma pneumoniae in Beijing, China: A multi-center study.
Authors: Guo DX, Hu WJ, Wei R, Wang H, Xu BP, Zhou W, Ma SJ, Huang H, Qin XG, Jiang Y, Dong XP, Fu XY, Shi DW, Wang LY, Shen AD, Xin DL Abstract Mycoplasma pneumoniae (M. pneumoniae) is one of the most common causes of community-acquired respiratory tract infections (RTIs). We aimed to investigate the prevalence of M. pneumoniae infection, antibiotic resistance and genetic diversity of M. pneumoniae isolates across multiple centers in Beijing, China. P1 protein was detected by Nested PCR to analyze the occurrence of M. pneumoniae in pediatric patients with RTI. M. pneumoniae isolates were cultured and analyzed by N...
Source: Bosnian Journal of Basic Medical Sciences - March 19, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Soft tissue grafts for dural reconstruction after meningioma surgery.
Authors: Velnar T, Gradisnik L Abstract The meninges are involved in various pathologies and are often directly or indirectly severed during surgical procedures, especially the dura mater. This can pose a real challenge for the surgeon, as a proper reconstruction of the meninges is important to prevent complications such as cerebrospinal fluid leak (CSF). A variety of techniques for dural reconstruction have been described, employing natural and artificial materials. A novel technique for dural reconstruction involves soft tissue grafts in the form of fibrous or fibromuscular flaps, which are placed on the dural de...
Source: Bosnian Journal of Basic Medical Sciences - March 18, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Outcomes and prognostic factors for patients with cervical esophageal cancer undergoing definitive radiotherapy or chemoradiotherapy.
This study investigated treatment outcomes and prognostic factors of survival in CEC patients undergoing definitive radiotherapy or concurrent chemoradiotherapy (CCRT). Ninety-one CEC patients were treated by intensity-modulated radiation therapy (IMRT) and three-dimensional conformal radiation therapy (3DCRT) between July 2007 and September 2017. The mean prescription dose was 64 Gy (range 54-70 Gy) delivered as 1.8-2.2 Gy per fraction per day, 5 days a week. Out of 91 patients, 34 received concurrent cisplatin-based chemotherapy (CT) including 18 patients who also received neoadjuvant CT. Overall survival (OS), locoregio...
Source: Bosnian Journal of Basic Medical Sciences - March 18, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Isoflurane exposure in infant rats acutely increases aquaporin 4 and does not cause neurocognitive impairment.
In this study, we aimed to determine whether a prolonged exposure to isoflurane in infant rats is associated with cognition and what effect this exposure has on AQP4 expression. Ten-day-old [postnatal day (P) 10] Wistar albino rats were randomly allocated to isoflurane group (n = 32; 1.5% isoflurane in 50% oxygen for 6 hours) or control group (n = 32; only 50% oxygen for 6 hours). Acute (P11) and long-term (P33) effects of 6-hour anesthetic isoflurane exposure on AQP4 expression were analyzed in whole brains of P11 and P33 rats by RT-qPCR and Western blot. Spatial learning and memory were assessed on P28 to P33 days by Mor...
Source: Bosnian Journal of Basic Medical Sciences - March 5, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Thymic stromal lymphopoietin levels are increased in patients with celiac disease.
In this study, we evaluated serum TSLP levels in patients with celiac disease (CD). The prospective study was conducted at a gastroenterology outpatient clinic between March 2018 and August 2018. Eighty-nine participants aged between 18 and 75 years were classified into following groups: 22 patients with newly diagnosed CD; 20 patients with CD who were compliant with a gluten-free diet (GFD); 32 patients with CD who were not compliant with a GFD; and 15 healthy controls. Demographic characteristics, disease duration, and selected biochemical and hematologic parameters were recorded and compared between groups. Median serum...
Source: Bosnian Journal of Basic Medical Sciences - March 5, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Upregulated expression of MNX1-AS1 long noncoding RNA predicts poor prognosis in gastric cancer.
In this study, we investigated the clinicopathological significance and biological function of MNX1-AS1 in gastric cancer (GC). The expression of MNX1-AS1 was analyzed by qRT-PCR in 96 GC and adjacent non-tumor tissues in relation to clinicopathological features and overall survival (OS) of patients, and in five human GC cell lines compared to a normal gastric epithelial cell line. Loss-of-function experiments using small interfering RNA (siRNA) targeting MNX1-AS1 (si-MNX1-AS1) were carried out in AGS and MGC-803 GC cell lines. Cell proliferation (CCK-8 assay), migration (Transwell) and invasion (Transwell Matrigel), and p...
Source: Bosnian Journal of Basic Medical Sciences - March 5, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Enhancement of bone consolidation using high-frequency pulsed electromagnetic fields (HF-PEMFs): An experimental study on rats.
Authors: Oltean-Dan D, Dogaru GB, Apostu D, Mester A, Benea HRC, Paiusan MG, Popa CO, Jianu EM, Bodizs GI, Berce C, Toader AM, Tomoaia G Abstract In vitro studies showed that high-frequency pulsed electromagnetic fields (HF-PEMFs) increase the activity/expression of early and late osteogenic markers and enhance bone mineralization. The main aim of this study was to investigate the in vivo effects of HF-PEMFs on fracture healing using a rat model. A femur fracture was established by surgery in 20 male Wistar rats. Titanium nails were implanted to reduce and stabilize the fracture. After surgery, 20 rats were equally...
Source: Bosnian Journal of Basic Medical Sciences - February 23, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Intervertebral disc tissue engineering: A brief review.
Authors: Stergar J, Gradisnik L, Velnar T, Maver U Abstract Intervertebral disc (IVD) degeneration (IDD) is associated with low back pain and significantly affects the patient's quality of life. Degeneration of the IVD alters disk height and the mechanics of the spine, leading to chronic segmental spinal instability. The pathophysiology of IVD disease is still not well understood. Current therapies for IDD include conservative and invasive approaches, but none of those treatments are able to restore the disc structure and function. Recently, tissue engineering techniques emerged as a possible approach to treat IDD,...
Source: Bosnian Journal of Basic Medical Sciences - February 7, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Feasibility and accuracy of a voxel-based neuronavigation system with 3D image rendering in preoperative planning and as a learning tool for young neurosurgeons, exemplified by the anatomical localization of the superior sagittal sinus.
In this study, we used a neuronavigation system with 3D volumetric image rendering to determine the anatomical relationship between the sagittal suture and the superior sagittal sinus (SSS) in patients with intracranial lesions. Furthermore, we discussed the applicability of such system in preoperative planning, residency training, and research. The study included 30 adult patients (18 female/12 male) who underwent a cranial computed tomography (CT) scan combined with venous angiography, for preoperative planning. The position of the sagittal suture in relation to the SSS was assessed in 3D CT images using an image guidanc...
Source: Bosnian Journal of Basic Medical Sciences - January 28, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Clinical outcomes after intentional replantation of permanent teeth: A systematic review.
In conclusion, the long-term success and survival rate of IR are likely dependent upon short extraoral time, reduced pocket depth, type of tooth, type of root-end filling material, and the prevention of atraumatic tooth root damage. PMID: 30684952 [PubMed - as supplied by publisher] (Source: Bosnian Journal of Basic Medical Sciences)
Source: Bosnian Journal of Basic Medical Sciences - January 28, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Limb-girdle muscular dystrophy due to GMPPB mutations: A case report and comprehensive literature review.
Authors: Sun L, Shen D, Xiong T, Zhou Z, Lu X, Cui F Abstract Mutations in the guanosine diphosphate mannose (GDP-mannose) pyrophosphorylase B (GMPPB) gene are rare. To date, 72 cases with GMPPB gene mutations have been reported. Herein, we reported a case of a 29-year-old Chinese male presenting with limb-girdle muscular dystrophy (LGMD) who was found to have two heterozygous GMPPB mutations. The patient had a progressive limb weakness for 19 years. His parents and elder brother were normal. On examination he had a waddling gait, and absent tendon reflexes in all four limbs. Electromyography showed myogenic damage...
Source: Bosnian Journal of Basic Medical Sciences - January 28, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Neurostructural correlates of strength decrease following total knee arthroplasty: A systematic review of the literature with meta-analysis.
Authors: Paravlic AH, Kovač S, Pisot R, Marusic U Abstract Recent literature suggests that alterations in both neural and structural components of the neuromuscular system are major determinants of knee extensor muscle weakness after total knee arthroplasty (TKA). Therefore, the goal of this study was to investigate the maximal voluntary strength (MVS), voluntary muscle activation (VMA), and the cross-sectional area (CSA) of the muscle, up to 33 months after the TKA.  We searched relevant scientific databases and literature for outcomes of interest, including quadriceps MVS, VMA, and CSA. Ten studies met the ...
Source: Bosnian Journal of Basic Medical Sciences - January 16, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research