Surgical anatomy of microsurgical 3-level anterior cervical discectomy and fusion C4-C7.
Authors: Gajski D, Dennis AR, Arnautovic KI Abstract Anterior cervical discectomy and fusion (ACDF) is one of the most common spinal procedures, frequently used for the treatment of cervical spine degenerative diseases. It was first described in 1958. Interestingly, to our knowledge, 3-level ACDF has not been previously published as a peer-reviewed video case with a detailed description of intraoperative microsurgical anatomy. In this video, we present the case of a 33-year-old male who presented with a combination of myelopathy (hyperreflexia and long tract signs in the upper and lower extremities) and bilateral r...
Source: Bosnian Journal of Basic Medical Sciences - June 23, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Targeting HER2 expression in cancer: New drugs and new indications.
Authors: Vranic S, Gatalica Z Abstract Functional activation of human epidermal growth factor receptor 2 (HER2) has been shown to strongly promote carcinogenesis, leading to the investigation of HER2-directed agents in cancers with HER2 genomic alterations. This has been best documented in the context of HER2 gene amplification in breast and gastric/gastroesophageal junction carcinomas for which several HER2-directed agents are available and have become a part of standard treatment regimens. Somatic HER2 gene mutations have been recently described at low frequency in a variety of human cancers and have emerged as a...
Source: Bosnian Journal of Basic Medical Sciences - June 13, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

SARS-CoV-2 infection of the nervous system: A review of the literature on neurological involvement in novel coronavirus disease (COVID-19).
In conclusion, SARS-CoV-2 infection can cause multiple neurological syndromes in a more complex presentation. Therefore, this review elucidated the involvement of the nervous system in SARS-CoV-2 infection and will hopefully help improve the management of COVID-19. PMID: 32530389 [PubMed - as supplied by publisher] (Source: Bosnian Journal of Basic Medical Sciences)
Source: Bosnian Journal of Basic Medical Sciences - June 13, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Combined inhibition of ACK1 and AKT shows potential toward targeted therapy against KRAS-mutant non-small-cell lung cancer.
Authors: Yu X, Liu J, Qiu H, Hao H, Zhu J, Peng S Abstract Non-small-cell lung cancer (NSCLC) with KRAS (Kirsten RAt Sarcoma 2 viral oncogene homolog) mutation has become a clinical challenge in cancer treatment as KRAS-mutant tumors are often resistant to conventional anti-tumor therapies. Activated CDC42-associated kinase 1 (ACK1), an activator of protein kinase B (AKT), is a promising target for KRAS-mutant tumor therapy, but the downstream ACK1 signaling remains poorly understood. The aim of this study was to evaluate the effectiveness of combined ACK1/AKT inhibition on the proliferation, migration, invasion, a...
Source: Bosnian Journal of Basic Medical Sciences - June 13, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

The first two months of COVID-19 pandemic in Bosnia and Herzegovina: Single-center experience.
Authors: Arapović J, Skočibušić S Abstract The novel coronavirus disease 2019 (COVID-19) pandemic caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is still progressing and has been recorded in more than 210 countries and territories worldwide. In Bosnia and Herzegovina, the first cases of COVID-19 were detected on 5th of March 2020 in the entity of Republic of Srpska and 9th of March 2020 in the entity of Federation of Bosnia and Herzegovina. Up until the 16th of May 2020, more than 2,200 COVID-19 cases were recorded in both entities, with a mortality rate of 5.8% (131 out of 2231 ca...
Source: Bosnian Journal of Basic Medical Sciences - May 30, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Lingual cyst with respiratory epithelium: The importance of differential diagnosis.
Authors: Cialente F, De Soccio G, Savastano V, Grasso M, Dello Spedale Venti M, Ralli M, Riminucci M, De Vincentiis M, Corsi A, Minni A PMID: 32464085 [PubMed - as supplied by publisher] (Source: Bosnian Journal of Basic Medical Sciences)
Source: Bosnian Journal of Basic Medical Sciences - May 30, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Lessons Learned from COVID-19 Pandemic in Italy.
Authors: Minni A, Ralli M, Candelori F, Cialente F, Ercoli L, Parlapiano C, Greco A, De Vincentiis M Abstract Since the COVID-19 outbreak, Italy has been one of the most affected countries in Europe and the second for number of deaths. In this commentary, we discuss some lessons that we learned as healthcare providers working in a large public hospital during the pandemic, with a special focus on the importance of infection containment and early diagnosis, the role of swab, serological tests, home isolation and individual protection devices,  and the available therapies and management indications to better fac...
Source: Bosnian Journal of Basic Medical Sciences - May 18, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

2020 consensus guideline for optimal approach to the diagnosis and treatment of HER2-positive breast cancer in Bosnia and Herzegovina.
The objectives of the guidelines include the standardization, harmonization and optimization of the procedures for the diagnosis, treatment and monitoring of patients with HER2-positive breast cancer, all of which should lead to an improvement in the quality of health care of mentioned patients. The initial treatment plan for patients with HER2-positive breast cancer must be made by a multidisciplinary tumor board comprised of at least: a medical oncologist, a pathologist, a radiologist, a surgeon, and a radiation oncologist/radiotherapist. PMID: 32415816 [PubMed - as supplied by publisher] (Source: Bosnian Journal of ...
Source: Bosnian Journal of Basic Medical Sciences - May 18, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Respiratory depression in the post-anesthesia care unit: Mayo Clinic experience.
This article briefly reviews the Mayo Clinic research experience and advances in clinical practice. From the implementation of a step-down model of discharge criteria in the post-anesthesia care unit (PACU), consisting of PACU nurses monitoring patients in predetermined periods for signs of respiratory depression, and delaying PACU discharge for patients who exhibit signs of respiratory depression, and early intervention in high-risk patients. Subsequent studies found that even a single episode of respiratory depression in the PACU was strongly associated with subsequent respiratory complications. Further, patient baseline...
Source: Bosnian Journal of Basic Medical Sciences - May 18, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Clinical Nocardia species: Identification, clinical characteristics, and antimicrobial susceptibility in Shandong, China.
Authors: Lu SH, Qian ZW, Mou PP, Xie L Abstract Nocardia is a pathogen responsible for a variety of clinical infections. Here, we aimed to investigate the species distribution, clinical manifestations, and antimicrobial susceptibility of Nocardia species in two tertiary general hospitals over 3 years in China. In this retrospective study, a total of 27 Nocardia species were isolated from 27 individuals between January 2017 and December 2019. Nocardia isolates were identified to species level by mass spectrometry and 16S rRNA PCR sequencing. Clinical data were collected from medical records. Antimicrobial susceptibi...
Source: Bosnian Journal of Basic Medical Sciences - May 18, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Clinical management of chronic mercury intoxication secondary to skin lightening products: A proposed algorithm.
Authors: Ramli FF Abstract Mercury is a toxic substance that is commonly used in skin lightening products. Various effects on humans have been observed, which affect both users and non-users. Many cases reported delayed diagnosis and treatment, even after weeks of hospitalization. The possible reasons are non-specific clinical manifestation and lack of awareness and knowledge regarding chronic mercury intoxication secondary to skin lightening products. A thorough history of mercury exposure is crucial. Physical assessment and relevant supporting tests are indicated to establish a diagnosis. Blood and urine mercury ...
Source: Bosnian Journal of Basic Medical Sciences - May 18, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

A three-dimensional computed tomography study to determine the ideal method for fluoroscopically-guided bone marrow aspiration from the iliac crest.
Authors: D'Souza RS, Li L, Leng S, Hunt C, Law L, Muir C, Eldrige J, Bydon M, Chi M, Shapiro S, Mauck WD, Qu W Abstract Bone marrow aspiration (BMA) through the iliac crest is potentially unsafe due to the vicinity of neurovascular structures in the greater sciatic notch. Our objective was to investigate the safety of a recently described BMA technique, specifically a trajectory from the posterior superior iliac spine (PSIS) to the anterior inferior iliac spine (AIIS). We conducted a chart review of 260 patients, analyzing three-dimensional reconstructed computed tomography images of the pelvis and sacrum to valida...
Source: Bosnian Journal of Basic Medical Sciences - May 18, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Progress in research on childhood T-cell acute lymphocytic leukemia, Notch1 signaling pathway, and its inhibitors: A review.
Authors: Fang-Fang Z, You Y, Wen-Jun L Abstract Childhood leukemia is a cancer seriously threatening children's life in China. The treatment of T-cell acute lymphocytic leukemia (T-ALL) is extremely difficult due to its early infiltration, poor sensitivity to chemotherapy, and susceptibility to drug resistance. At the same time, the traditional intensive chemotherapy regimens cause great damage to children, so it is highly important to search for the targeted drugs and develop precise individualized treatment of child patients. The Notch signaling pathway is involved the pathogenesis of T-ALL, and there are activat...
Source: Bosnian Journal of Basic Medical Sciences - May 18, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Chronic maxillary sinusitis of dental origin and oroantral fistula: The results of combined surgical approach in an Italian university hospital.
Authors: Galli M, De Soccio G, Cialente F, Candelori F, Federici FR, Ralli M, De Vincentiis M, Minni A Abstract Unilateral chronic maxillary sinusitis is a possible complication of odontogenic disease or dental treatment and is mainly due to the development of an oroantral fistula (OAF). The management of chronic maxillary sinusitis of dental origin requires a combined treatment via endoscopic sinus surgery (ESS) and intraoral surgical treatment of the odontogenic source. The aim of this study is to present the results of our university hospital unit in the treatment and follow-up of a case series of 34 patients tr...
Source: Bosnian Journal of Basic Medical Sciences - May 4, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Monomeric C-reactive protein affects cell injury and apoptosis through activation of p38 MAPK in human coronary artery endothelial cells.
Authors: Zhang Y, Cao H Abstract C-reactive protein (CRP) is an important predictor of cardiovascular events and plays a role in vascular inflammation and vessel damage. The aim of this study was to investigate the effect of pentameric CRP (pCRP) and monomeric CRP (mCRP) on the production of atherosclerosis-related factors in cultured human coronary artery endothelial cells (HCAECs). HCAECs were treated with pCRP, mCRP, p38 mitogen-activated protein kinase (MAPK) inhibitor SB203580, or transfected with p38 MAPK siRNA. Western blotting was performed to detect the expression of vascular endothelial growth factor (VEG...
Source: Bosnian Journal of Basic Medical Sciences - May 4, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

High MTHFR promoter methylation levels in men confer protection against ischemic stroke.
In this study, we first aimed to clarify the association between DNA methylation levels in the MTHFR promoter and the risk of IS, followed by the analysis of potential interactions between environmental factors and DNA methylation levels that affect IS risk. We recruited 164 patients with hypertension and IS (case group) and 345 age- and sex-matched patients with hypertension only (control group). Demographic and clinical information was obtained using questionnaires, and blood samples were collected for biochemical analyses. Fluorescence quantitative methylation-specific PCR (qMSP) was used to detect MTHFR promoter methyl...
Source: Bosnian Journal of Basic Medical Sciences - May 4, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Uvulopalatopharyngoplasty and barbed reposition pharyngoplasty with and without hyoid suspension for obstructive sleep apnea hypopnea syndrome: A comparison of long-term functional results.
Authors: Antonio Minni, Cialente F, Ralli M, Colizza A, Lai Q, Placentino A, Franco M, Rossetti V, De Vincentiis M Abstract Obstructive sleep apnea hypopnea syndrome (OSAHS) is a common condition; when conservative approaches are not effective, surgical techniques aimed at reducing the airway obstruction effect are used. This retrospective study aimed at comparing the functional outcomes in patients with OSAHS undergoing uvulopalatopharyngoplasty (UPPP) according to Fairbanks and barbed reposition pharyngoplasty (BRP) according to Mantovani, with or without hyoid suspension (HS). One-hundred twenty-two consecutive ...
Source: Bosnian Journal of Basic Medical Sciences - April 30, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Upper limb principal arteries variations: A cadaveric study with terminological implication.
Authors: Konarik M, Musil V, Baca V, Kachlik D Abstract Although the variability of the upper limb arteries is a clinically important problem, the prevalence varies across the existing studies and the classification is rather complicated, uncoined, and sometimes even unclear for its simple and direct understanding and usage. Multiple case reports appearing in the last years apply incorrect, inappropriate, and sometimes misleading terminology. Here we performed an anatomical cadaveric study of the variability of the arteries of the upper limb, namely the axilla, arm and forearm, in 423 upper limbs (Central European ...
Source: Bosnian Journal of Basic Medical Sciences - April 30, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity.
In conclusion, abnormally expressed miR-145 may be involved in hydrosalpinx-induced ER defects by regulating inflammatory response. PMID: 32343942 [PubMed - as supplied by publisher] (Source: Bosnian Journal of Basic Medical Sciences)
Source: Bosnian Journal of Basic Medical Sciences - April 30, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery.
Authors: Milutinović A, Zorc-Pleskovič R Abstract The patency of the vein graft in coronary artery bypass grafting could be dependent on conventional (vsO) or endoscopic (vsE) harvesting and on the hypoxic damage of endothelial cells. We aimed to evaluate both surgical techniques according to endothelial loss that occurs in the time between harvesting and implantation. Twenty-six saphenous veins were divided into vsO (n = 16) and vsE (n = 10) group. Three samples were taken from each vein. The first sample was taken after removal, the second before implantation of the distal part, and the third before the implant...
Source: Bosnian Journal of Basic Medical Sciences - March 29, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Immunohistochemical study of dental pulp cells with 3D collagen type I gel in demineralized dentin tubules in vivo.
This study aimed to evaluate the growth and differentiation potential of DPCs cultured inside demineralized dentin tubules in vivo. Six green fluorescent protein (GFP)-transgenic rats (body weight 100 g each) and thirty-two Sprague-Dawley (SD) male rats (body weight 250 g each) were used for DPC collection and dentin tubules preparation and transplantation, respectively. Third-passage DPCs with or without collagen gels were loaded into demineralized dentin tubules. Both types of grafts were transplanted into the rectus abdominis muscles of SD rats and were harvested after 21 days. The expression of alkaline phosphatase (AL...
Source: Bosnian Journal of Basic Medical Sciences - March 29, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Can telomere length predict bone health? A review of current evidence.
Authors: Wong SK, Ima-Nirwana S, Chin KY Abstract Telomeres are repetitive DNA sequences located at the end of chromosomes, which serve as a protective barrier against chromosomal deterioration during cell division. Approximately 50-200 base pairs of nucleotides are lost per cell division and new repetitive nucleotides are added by the enzyme telomerase allowing telomere maintenance. Telomere shortening has been proposed as an indicator for biological ageing, but its relationship with age-related osteoporosis is ambiguous. We summarize the current evidence on the relationship between telomere length and bone health...
Source: Bosnian Journal of Basic Medical Sciences - March 12, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Long non-coding RNA PVT1 regulates the migration of hepatocellular carcinoma HepG2 cells via miR-3619-5p/MKL1 axis.
In this study, we investigated the relationship between PVT1 and MKL1 as a novel regulatory mechanism underlying HCC progression. We used HepG2 and Cos‑7 cell lines. Transfection experiments with miR-3619-5p mimics/inhibitor, PVT1, siRNA-PVT1, MKL1, or siRNA-MKL1 were performed. RNA and protein levels were analyzed by quantitative reverse transcription PCR and Western blot, respectively. Cell migration was assessed by Transwell assay. Luciferase assays, RNA-FISH, RNA immunoprecipitation, and chromatin immunoprecipitation assays were performed to confirm the interaction between PVT1, miR-3619-5p, and MKL1 in HCC cells. Ov...
Source: Bosnian Journal of Basic Medical Sciences - March 12, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Asymptomatic neurotoxicity of amyloid β-peptides (Aβ1-42 and Aβ25-35) on mouse embryonic stem cell-derived neural cells.
In this study, we attempted to induce oxidative stress in transgenic 46C mouse embryonic stem cell-derived neurons via treatment with Ab peptides (Aβ1-42 and Aβ25-35). 46C neural cells were generated by promoting the formation of multicellular aggregates, embryoid bodies (EBs) in the absence of leukemia inhibitory factor (LIF), followed by the addition of all-trans retinoic acid (ATRA) as the neural inducer. Mature neuronal cells were exposed to different concentrations of Aβ1-42 and Aβ25-35 for 24 h. Morphological changes, cell viability, and intracellular ROS production were assessed. We fou...
Source: Bosnian Journal of Basic Medical Sciences - March 12, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Neurological outcome in patients after successful resuscitation in out-of-hospital settings.
Authors: Marinšek M, Sinkovič A, Šuran D Abstract Neurological outcome is an important determinant of death in admitted survivors after out-of-hospital cardiac arrest (OHCA). Studies demonstrated several significant pre-hospital predictors of ischemic brain injury (time to resuscitation, time of resuscitation, and cause of OHCA). Our aim was to evaluate the relationship between post-resuscitation clinical parameters and neurological outcome in OHCA patients, when all recommended therapeutic strategies, including hypothermia, were on board. We retrospectively included consecutive 110 patients, admitte...
Source: Bosnian Journal of Basic Medical Sciences - March 12, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Neural stem cell-conditioned medium ameliorates A β25-35-induced damage in SH-SY5Y cells by protecting mitochondrial function.
Neural stem cell-conditioned medium ameliorates Aβ25-35-induced damage in SH-SY5Y cells by protecting mitochondrial function. Bosn J Basic Med Sci. 2020 Feb 24;: Authors: Jia G, Diao Z, Liu Y, Sun C, Wang C Abstract Inhibition of amyloid β (Aβ)-induced mitochondrial damage is considered crucial for reducing the pathological damage in Alzheimer's disease (AD). We evaluated the effect of neural stem cell-conditioned medium (NSC-CDM) on Aβ25-35-induced damage in SH-SY5Y cells. An in vitro model of AD was established by treating SH-SY5Y cells with 40 μM Aβ25-35 for 24 h. SH-SY5Y...
Source: Bosnian Journal of Basic Medical Sciences - March 12, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Inflammation-related cytokines and their roles in gastroenteropancreatic neuroendocrine neoplasms.
In this study, we explored the immunohistochemical expression of tumor necrosis factor alpha (TNF-α), interleukin 1 beta (IL-1β), IL-2, and IL-6 in tissues from 43 GEP-NEN patients with tumors of gastric, duodenal, ileal, appendical, and colonic origin. The immunohistochemical expression of TNF-α was increased in tumor groups with high proliferation rates (Ki67; p = 0.034), as well as in those with higher tumor grades (p = 0.05). Moreover, the immunohistochemical expression of TNF-α positively correlated with death outcomes (p = 0.016). Expression of IL-6, IL-1β, and IL-2 displayed similar immun...
Source: Bosnian Journal of Basic Medical Sciences - March 12, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Knockdown of TPT1-AS1 inhibits cell proliferation, cell cycle G1/S transition, and epithelial-mesenchymal transition in gastric cancer.
In this study, we analyzed the expression of TPT1-AS1 in GC tissues and cell lines and performed functional and mechanistic analysis of TPT1-AS1 effects on GC cell proliferation, migration, and invasion. TPT1-AS1 expression was determined in 76 pairs of GC tissues vs. matched adjacent normal tissues and in four GC cell lines (SGC-7901, AGS, BGC-823, and MGC-803) vs. GES-1 cell line by quantitative reverse transcription PCR. SGC-7901 and MGC-803 cells were transfected with small interfering RNA or scrambled negative control, and cell proliferation, colony formation, migration, invasion and cell cycle assays were performed. ...
Source: Bosnian Journal of Basic Medical Sciences - March 12, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Laryngeal tuberculosis in renal transplant recipients: A case report and review of the literature.
Authors: Cialente F, Grasso M, Ralli M, De Vincentiis M, Minni A, Agolli G, Dello Spedale Venti M, Riminucci M, Corsi A, Greco A Abstract Renal allograft recipients are at greater risk of developing tuberculosis than the general population. A woman with a kidney transplant was admitted to the emergency department with high temperature, dysphonia, odynophagia, and asthenia. The final diagnosis was laryngeal tuberculosis. Multidisciplinary collaboration enabled accurate diagnosis and successful treatment. Laryngeal tuberculosis should be considered in renal allograft recipients with hoarseness. A more rapid diagnosis...
Source: Bosnian Journal of Basic Medical Sciences - March 12, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Post-operative functional neurological symptom disorder after anesthesia.
We report a case series consisting of six patients who underwent a general anesthetic at a tertiary referral hospital and experienced FNSD in the immediate post-anesthetic period. Life-threatening causes were excluded based on benign physical exam findings and knowledge of past history. Five of six cases manifested with FNSD only in the immediate post-operative setting after exposure to anesthesia, and never otherwise experienced these symptoms during their normal daily lives. MEDLINE and Google Scholar were searched through October 2019 using a highly-sensitive search strategy and identified 38 published cases of post-ane...
Source: Bosnian Journal of Basic Medical Sciences - February 21, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

P2X7R as an independent prognostic indicator in gastric cancer.
Authors: Calik I, Calik M, Sarikaya B, Ozercan IH, Arslan R, Artas G, Dagli AF Abstract Gastric cancer is one of the foremost causes of cancer related death around the world. The P2X7 receptor (P2X7R), a member of the P2X receptor subfamily of P2 receptors, is a unique molecule that has been shown to affect tumor growth and progression as well as various inflammatory processes, including proliferation of T lymphocytes, release of cytokines, and production of free oxygen radicals. P2X7R has been established as a prognostic parameter in some cancers and, recently, it has been investigated in the development of new ta...
Source: Bosnian Journal of Basic Medical Sciences - February 21, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Interventions of a clinical pharmacist in a medical intensive care unit - a retrospective analysis.
Authors: Cvikl M, Sinkovič A Abstract Several studies demonstrated a significant decrease in prescription errors, adverse drug events, treatment costs and improved patient outcomes, when a clinical pharmacist (CP) was a full member of a multidisciplinary team in the intensive care unit (ICU). Our aim was to evaluate the activities of a CP, included in a 12-bed medical ICU team of a university hospital in the course of several months. We conducted a retrospective analysis of all the CP's interventions from March to November 2017, carried out and documented after reviewing and discussing patients' medical data with ...
Source: Bosnian Journal of Basic Medical Sciences - February 21, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Significance of chromogranin A and synaptophysin in pancreatic neuroendocrine tumors.
Authors: Tomita T Abstract The two most commonly used immunohistochemical markers for neuroendocrine cells and their tumors are chromogranin A (CgA) and synaptophysin (SPY). CgA is a marker for neuroendocrine secretory granules of four pancreatic hormones and gastrin while SPY is a marker for synaptic vesicles in neuroendocrine cells, which release classic neurotransmitters such as acetylcholine and others. CgA is involved in synthesis and secretion of peptide hormones through exocytosis while function of SPY is elusive. Thirty-five pancreatic neuroendocrine tumors (Pan-NETs) were studied, consisting of 14 insulino...
Source: Bosnian Journal of Basic Medical Sciences - February 7, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

The key bacterial cell division protein FtsZ as a novel antibacterial drug target.
Authors: Rahman M, Wang P, Wang N, Chen Y Abstract The number of multidrug-resistant bacterial strains is currently increasing; thus, the determination of drug targets for the development of novel antimicrobial drugs is urgently needed. FtsZ, the prokaryotic homolog of the eukaryotic tubulin, is a GTP-dependent prokaryotic cytoskeletal protein that is conserved among most bacterial strains. In vitro studies revealed that FtsZ self-assembles into dynamic protofilaments or bundles, and it forms a dynamic Z-ring at the center of the cell, leading to septation and consequent cell division. The potential role of FtsZ in...
Source: Bosnian Journal of Basic Medical Sciences - February 7, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

The natural adaptive evolution of cancer: The metastatic ability of cancer cells.
Authors: Olteanu GE, Mihai IM, Bojin F, Gavriliuc O, Paunescu V Abstract The ability of cancer to adapt renders it one of the most challenging pathologies of all time. It is the most dreaded pathological entity because of its capacity to metastasize to distant sites in the body, and 90% of all cancer-related deaths recorded to date are attributed to metastasis. Currently, three main theories have been proposed to explain the metastatic pathway of cancer: the epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) hypothesis (1), the cancer stem cell hypothesis (2), and the macrophage-can...
Source: Bosnian Journal of Basic Medical Sciences - February 7, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

DNA methylation of AHCY may increase the risk of ischemic stroke.
This study aimed to determine the correlation between DNA methylation of the gene encoding S-adenosylhomocysteine hydrolase (AHCY) and the risk of ischemic stroke in 64 ischemic stroke patients and 138 patients with traumatic brain injury (control group). The methylation level of AHCY was analyzed using quantitative methylation-specific polymerase chain reaction. Statistically significant differences in AHCY methylation levels were observed between the case group [medians (interquartile range): 0.13% (0.09%, 0.27%)] and the control group [0.06% (0.00%, 0.17%), p
Source: Bosnian Journal of Basic Medical Sciences - February 7, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

WD-3 inhibits the proliferation of breast cancer cells by regulating the glycolytic pathway.
This study aimed to investigate the effect of WD-3 on proliferation, glycolysis, and hexokinase 2 expression in breast cancer cells. Four breast cancer cell lines (MDA-MB-231, BT-549, MCF-7, and MCF-7/ADR-RES) were treated with different concentrations of WD-3 compared with blank control (phosphate-buffered saline). Each of the breast cancer cell lines was also divided into WD-3, paclitaxel, and blank control group. Cell proliferation and morphology were assessed by MTT assay, nuclear Hoechst 33258 staining, or immunofluorescence. Apoptosis was analyzed by flow cytometry. High performance liquid chromatography was used for...
Source: Bosnian Journal of Basic Medical Sciences - February 7, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Cisplatin inhibits the proliferation of Saos-2 osteosarcoma cells via the miR-376c/TGFA pathway.
In this study, we investigated the mechanism underlying inhibitory effects of cisplatin on growth and proliferation of osteosarcoma cells. Osteosarcoma and normal skeletal muscle tissues were collected from 26 patients by biopsy. TGFA was silenced or overexpressed in Saos-2 osteosarcoma cells by transfection with TGFA-shRNA or TGFA ORF clone, respectively. MiR-376c was inhibited or overexpressed by transfection of Saos-2 cells with miR-376c sponge or miR-376c mimics, respectively. Cell growth was analyzed by MTT assay and cell proliferation by BrdU assay. MiR-376c and TGFA mRNA expression was detected by quantitative rever...
Source: Bosnian Journal of Basic Medical Sciences - February 7, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Concordance of PD-L1 expression and CD8+ TIL intensity between NSCLC and synchronous brain metastases.
This study assessed the concordance of PD-L1 expression on tumor cells and tumor infiltrating lymphocytes (TILs) and CD8+ TIL intensity between primary lung tumors and synchronous BMs from 24 NSCLC patients. PD-L1, CD3, and CD8 positivity was determined by immunohistochemistry (IHC). PD-L1 scoring was based on the proportion of tumor cells with membranous expression of PD-L1 and the cutoff values
Source: Bosnian Journal of Basic Medical Sciences - February 1, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Overexpression of microRNA-129-5p in glioblastoma inhibits cell proliferation, migration, and colony-forming ability by targeting ZFP36L1.
In this study, we investigated the role of miR-129-5p and miR-129-5p/ZFP36L1 axis in GBM tumorigenesis. Analysis of GSE103228 microarray data from the GEO database showed that miR-129-5p was significantly downregulated in GBM vs. normal brain tissues. Quantitative reverse transcription PCR analysis of miR-129-5p expression in seven GBM cell lines (LN229, A172, U87, T98G, U251, H4, and LN118) vs. normal human astrocytes (NHA) showed miR-129-5p was significantly downregulated in GBM cells. Overexpression of miR-129-5p in LN229 and A172 cells significantly suppressed cell proliferation, migration, invasion, and colony-forming...
Source: Bosnian Journal of Basic Medical Sciences - February 1, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Circulating long noncoding RNAs H19 and GAS5 are associated with type 2 diabetes but not with diabetic retinopathy: A preliminary study.
Authors: Fawzy MS, Abdelghany AA, Toraih EA, Mohamed AM Abstract Recently, a wide range of biological and pathological roles of long noncoding RNAs (lncRNAs) have been discovered. However, the potential role of circulating lncRNAs H19 and GAS5 in type 2 diabetes mellitus (T2DM) and diabetic retinopathy (DR) is not clear. Here, we assessed the plasma levels of H19 and GAS5 lncRNAs in T2DM patients with/without DR and evaluated if H19 and GAS5 pre-treatment plasma levels are a predictor of early response to a single aflibercept dose in DR subgroup. Plasma lncRNA expression profiles of 119 T2DM patients (66 with DR an...
Source: Bosnian Journal of Basic Medical Sciences - February 1, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

MicroRNA-126 enhances the biological function of endothelial progenitor cells under oxidative stress via PI3K/Akt/GSK-3 β and ERK1/2 signaling pathways.
MicroRNA-126 enhances the biological function of endothelial progenitor cells under oxidative stress via PI3K/Akt/GSK-3β and ERK1/2 signaling pathways. Bosn J Basic Med Sci. 2020 Jan 30;: Authors: Wu Q, Qi B, Duan X, Ming X, Yan F, He Y, Bu X, Sun S, Zhu H Abstract Endothelial progenitor cell (EPC) transplantation is a safe and effective method to treat acute myocardial infarction (AMI). However, oxidative stress leads to the death of a large number of EPCs in the early stage of transplantation, severely weakening the therapeutic effect. Previous studies demonstrated that microRNAs (miRNAs) regula...
Source: Bosnian Journal of Basic Medical Sciences - February 1, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Galectin-1 reduces the severity of dextran sulfate sodium (DSS)-induced ulcerative colitis by suppressing inflammatory and oxidative stress response.
Authors: Arda-Pirincci P, Aykol-Celik G Abstract Ulcerative colitis is an inflammatory bowel disease that affects a large number of people around the world. Galectin-1 is a β-galactoside-binding lectin with a broad range of biological activities. The effects of galectin-1 on dextran sulfate sodium (DSS)-induced ulcerative colitis in vivo is not clear. We investigated the effect of galectin-1 on colon morphology, cell proliferation, oxidative stress, antioxidant system, and proinflammatory/antiinflammatory cytokines in a DSS-induced mouse model of ulcerative colitis. Thirty-two C57BL/6 mice were randomly assign...
Source: Bosnian Journal of Basic Medical Sciences - February 1, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Diagnostic performance of LI-RADS version 2018 in differentiating hepatocellular carcinoma from other hepatic malignancies in patients with hepatitis B virus infection.
Authors: Shao S, Liang Y, Kuang S, Chen J, Shan Q, Yang H, Zhang Y, Wang B, J Fowler K, Wang J, B Sirlin C Abstract The diagnostic performance of the Liver Imaging Reporting and Data System (LI-RADS) in differentiating hepatocellular carcinoma (HCC) from other hepatic malignancies has not been investigated in Chinese patients with chronic liver disease from hepatitis B virus (HBV) infection. The aim of this study was to evaluate the accuracy of the LI-RADS version 2018 in differentiating HCC, intrahepatic cholangiocarcinoma (ICCA), and combined HCC-cholangiocarcinoma (cHCC-CCA) in Chinese patients with HBV infectio...
Source: Bosnian Journal of Basic Medical Sciences - February 1, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Lysozyme in the treatment of non-infectious sore throat.
Authors: Glamočlija U, Mehić M, Šukalo A, Tanović Avdić A, Džananović Jaganjac J PMID: 31999941 [PubMed - as supplied by publisher] (Source: Bosnian Journal of Basic Medical Sciences)
Source: Bosnian Journal of Basic Medical Sciences - February 1, 2020 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Androgen effect on connexin expression in the mammalian female reproductive system: a systematic review.
Authors: Mohd Kamal DA, Ibrahim SF, Mokhtar MH Abstract The functions of androgen and connexin in the mammalian female reproductive system are suggested to be related. Previous research has shown that androgen affects connexin expression and localization in the female reproductive system, altering its function. However, no definitive conclusion on their cause-effect relationship has been drawn yet. In addition, a high prevalence of women with polycystic ovary syndrome (PCOS), who are characterized by elevated androgen levels and failure of ovulation, has prompted the studies on the relationship between androgen and...
Source: Bosnian Journal of Basic Medical Sciences - December 29, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Serum patterns of mir-23a and mir-181b in irritable bowel syndrome and colorectal cancer - A pilot study.
Authors: Chira A, Muresan MS, Braicu C, Budisan L, Raduly L, Chira RI, Dumitrascu DL, Berindan-Neagoe I Abstract Emerging evidence demonstrates that microRNAs (miRNAs) could serve as reliable biomarkers of inflammation and oncogenesis. The aim of this study was to determine whether miR-23a and miR-181b were suitable as biomarkers of irritable bowel syndrome (IBS) and colorectal cancer (CRC). Forty patients with IBS (29 females, 11 males), 33 with CRC (14 females, 19 males), and 33 healthy controls (17 females, 16 males) were prospectively included. Serum levels of miRNAs were evaluated by quantitative real-time PCR...
Source: Bosnian Journal of Basic Medical Sciences - December 29, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Vancomycin-releasing cross-linked collagen sponges as wound dressings.
Authors: Hartinger JM, Lukáč P, Mitáš P, Mlček M, Popková M, Suchý T, Šupová M, Závora J, Adámková V, Benáková H, Slanař O, Šíma M, Bartoš M, Chlup H, Grus T Abstract The study presents a novel vancomycin-releasing collagen wound dressing derived from Cyprinus carpio collagen type I cross-linked with carbodiimide which retarded the degradation rate and increased the stability of the sponge. Following lyophilization, the dressings were subjected to gamma sterilization. The structure was evaluated via scanning ...
Source: Bosnian Journal of Basic Medical Sciences - December 1, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Percutaneous coronary intervention assisted by invasive mechanical ventilation and intra-aortic balloon pump for acute myocardial infarction with cardiogenic shock: Retrospective cohort study and meta-analysis.
Authors: Liu Y, Li CP, Lu PJ, Wang XY, Xiao JY, Gao MD, Wang JX, Li XW, Zhang N, Li CJ, Ma J, Gao J Abstract The aim of this study was to compare the mortality outcome in patients with acute myocardial infarction and cardiogenic shock who were treated with percutaneous coronary intervention (PCI) assisted by intra-aortic balloon pump (IABP) + invasive mechanical ventilation (IMV) with historical controls. From January 1, 2016 to June 1, 2017, 60 patients were retrospectively enrolled at Tianjin Chest Hospital. Out of these, 88.3% of patients achieved thrombolysis in myocardial infarction flow 3 after PCI. The all-c...
Source: Bosnian Journal of Basic Medical Sciences - December 1, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research

Human brucellosis in pregnancy - an overview.
Authors: Bosilkovski M, Arapović J, Keramat F Abstract Human brucellosis during pregnancy is characterized by significantly less pronounced adverse obstetric outcomes than in animals, but with remarkably more adverse obstetric outcomes when compared to healthy pregnant women. Seroprevalence of brucellosis in pregnancy and cumulative incidence of brucellosis cases per 1000 delivered obstetrical discharges in endemic regions were reported to be 1.5-12.2% and 0.42-3.3, respectively. Depending on the region, frequency of pregnant women in the cohorts of patients with brucellosis was from 1.5% to 16.9%. The most common...
Source: Bosnian Journal of Basic Medical Sciences - December 1, 2019 Category: General Medicine Tags: Bosn J Basic Med Sci Source Type: research