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Efficient and specific gene knockdown by small interfering RNAs produced in bacteria
Nature Biotechnology 31, 350 (2013).
doi:10.1038/nbt.2537
Authors: Linfeng Huang, Jingmin Jin, Padraig Deighan, Evgeny Kiner, Larry McReynolds & Judy Lieberman
Synthetic small interfering RNAs (siRNAs) are an indispensable tool to investigate gene function in eukaryotic cells and may be used for therapeutic purposes to knock down genes implicated in disease. Thus far, most synthetic siRNAs have been produced by chemical synthesis. Here we present a method to produce highly potent siRNAs in Escherichia coli. This method relies on ectopic expression of p19, an siRNA-binding protein found in a plant RNA virus. Whe...
Source: Nature Biotechnology - March 10, 2013 Category: Biotechnology Authors: Linfeng HuangJingmin JinPadraig DeighanEvgeny KinerLarry McReynoldsJudy Lieberman Tags: Research Source Type: research
