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Infectious Disease: Gastroenteritis

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Total 121 results found since Jan 2013.

Viruses, Vol. 14, Pages 359: Encephalomyocarditis Virus 2A Protein Inhibited Apoptosis by Interaction with Annexin A2 through JNK/c-Jun Pathway
In this study, the 2A protein was expressed in Escherichia coli in order to find interacting cell proteins. A pull down assay, coupled with mass spectrometry, revealed that the 2A protein possibly interacted with annexin A2. Co-immunoprecipitation assays and confocal imaging analysis further demonstrated that the 2A protein interacted with annexin A2 in cells. In reducing the expression of annexin A2 by siRNA, the ability of the 2A protein to inhibit apoptosis was weakened and the proliferation of EMCV was slowed down. These results suggest that annexin A2 is closely related to the inhibition of apoptosis by 2A. Furthermor...
Source: Viruses - February 9, 2022 Category: Virology Authors: Ruochan Han Lin Liang Tong Qin Sa Xiao Ruiying Liang Tags: Article Source Type: research

Development of transgenic okra (Abelmoschus esculentus L. Moench) lines having RNA mediated resistance to Yellow vein mosaic virus (Geminiviridae)
J Virol Methods. 2022 Jan 6;301:114457. doi: 10.1016/j.jviromet.2022.114457. Online ahead of print.ABSTRACTBegomovirus Yellow vein mosaic virus causes severe yield losses in okra and even the resistant lines developed through conventional breeding show susceptibility at various levels. This paper describes the development of YVMV resistant lines through RNAi strategy. A universal ihpRNA construct harbouring βC1 ORF from the β-satellite of the begomovirus was designed using pRNAi-LIC plasmid. Complementarity checks in sequence databases had shown no off-target effects by the target region and the success of siRNA in inter...
Source: Journal of Virological Methods - January 9, 2022 Category: Virology Authors: Kelkar Vipul Ganesh Deepu Mathew Rehna Augustine Soni K B Swapna Alex Shylaja M R Anita Cherian K Source Type: research

< i > Trichinella spiralis < /i > : Knockdown of gamma interferon inducible lysosomal thiol reductase (GILT) results in the reduction of worm burden
by Hong Fei, Muhammad Ali-ul-Husnain Naqvi, Sana Zahra Naqvi, Lixin Xu, Xiaokai Song, Xiangrui Li, Ruofeng YanTrichinella spiralis is mammalian skeletal muscles parasite which may cause trichinellosis in animals and humans. Gamma interferon inducible lysosomal thiol reductase (GILT) is a widespread superfamily which plays key role in processing and presentation of MHC class II restricted antigen by catalyzing disulfide bond reduction. There are no reports about GILT inT.spiralis. In present study, GILT fromT.spiralis (Tsp-GILT) was cloned, analyzed by multiple-sequence alignment, and predicted by 3D structure model. Recomb...
Source: PLoS Neglected Tropical Diseases - November 30, 2021 Category: Tropical Medicine Authors: Hong Fei Source Type: research

Possibility for double optimization of siRNA intracellular delivery efficiency and antibacterial activity: Structure screening of pH-sensitive triblock amphiphilic polycation micelles
Colloids Surf B Biointerfaces. 2021 Oct 26;209(Pt 2):112178. doi: 10.1016/j.colsurfb.2021.112178. Online ahead of print.ABSTRACTOptimal combination of hydrophobic-hydrophilic balance, proton buffering and electrostatic interaction is the key issue for designing polycations as efficient gene vectors and antibacterial agents. Herein, we screened a series of pH-sensitive quaternary ammonium-based amphiphilic triblock copolymers, mPEG2k-P(DPAa/DMAb)-PQAc (TDDE-x), which had different pKa values and proton buffering capacities. Significantly, we found that both the highest siRNA intracellular delivery efficiency and the stronge...
Source: Colloids and Surfaces - November 6, 2021 Category: Biotechnology Authors: Yufeng Zhang Yanliang Dong Junhui Zhou Wenjun Huang Yidi Wu Shuyue Zhao Yongli Shi Suping Bai Chunhui Li Yuanyu Huang Anjie Dong Source Type: research

Escherichia coli infection activates the production of IFN- α and IFN-β via the JAK1/STAT1/2 signaling pathway in lung cells
Amino Acids. 2021 Sep 15:1-14. doi: 10.1007/s00726-021-03077-6. Online ahead of print.ABSTRACTEscherichia coli infections can result in lung injury, which may be closely linked to the induction of interferon secretion. The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway is one of most important pathways that regulate interferon production. Thus, the present study aimed to dissect whether E. coli infections can regulate interferon production and the underlying mechanisms. For this aim, two lung cell lines, a human bronchial epithelial cell line transformed with Ad12-SV40 2B (BEAS-2b) and a...
Source: Amino Acids - September 15, 2021 Category: Biochemistry Authors: Yan Jin Ziyi Jia Quan Cai Yanfei Sun Zhi Liu Source Type: research

Properdin Deficiency Impairs Phagocytosis and Enhances Injury at Kidney Repair Phase Post Ischemia –Reperfusion
This study revealed that properdin knockout (PKO) mice exhibited greater injury in renal function and histology than wild-type (WT) mice post 72-h IR, with more apoptotic cells and macrophages in tubular lumina, increased active caspase-3 and HMGB1, but better histological structure at 24 h. Raised erythropoietin receptor by IR was furthered by PKO and positively correlated with injury and repair markers. Properdin in WT kidneys was also upregulated by IR, while H2O2-increased properdin in TECs was reduced by its small-interfering RNA (siRNA), with raised HMGB1 and apoptosis. Moreover, the phagocytic ability of WT TECs, an...
Source: Frontiers in Immunology - September 6, 2021 Category: Allergy & Immunology Source Type: research

siRNA-based identification of IBD-related targets in human monocyte-derived dendritic cells
J Immunol Methods. 2021 Apr 20:113058. doi: 10.1016/j.jim.2021.113058. Online ahead of print.ABSTRACTInflammatory bowel disease (IBD) is thought to be caused by an aberrant host response to the commensal enteric flora in genetically susceptible individuals. Dendritic cells (DCs) play a key role in the regulation of this response as they sample gut commensals. In healthy individuals DCs actively contribute to tolerance upon recognition of these resident bacteria, whereas in individuals with IBD, DCs will initiate an inflammatory response. To mimic the disease response in vitro, human monocyte-derived DCs were matured with E...
Source: Journal of Immunological Methods - April 23, 2021 Category: Allergy & Immunology Authors: Diane Bosman-Schluep Remko de Pril Banu Verbaken Ana ïs Legent Jan Stallen Esther C de Jong Richard A J Janssen Source Type: research

Ac-HSP20 Is Associated With the Infectivity and Encystation of Acanthamoeba castellanii
This study aimed to determine the biological functions of heat shock protein (HSP)-20 of A. castellanii (Ac-HSP20) involved in the maintenance of life cycle and the infectivity of A. castellanii. Immunoscreening A. castellanii cDNA library with A. castellanii infected rabbit sera identified three positive clones, one of them was a putative heat shock protein (Ac-HSP20). The recombinant 23 kDa Ac-HSP20 protein (rAc-HSP20) was successfully expressed in Escherichia coli BL21 (DE3) and purified using metal affinity chromatography. The rabbits immunized with rAc-HSP20 produced high titer antibody (1:25,600). Immunolocalization ...
Source: Frontiers in Microbiology - January 12, 2021 Category: Microbiology Source Type: research

Long Non-coding RNAs RN7SK and GAS5 Regulate Macrophage Polarization and Innate Immune Responses
This study elucidates the regulatory role of lncRNAs in monocyte to Mφ differentiation, M1/M2 dichotomy and innate immune responses. Expression profiling of eighty-eight lncRNAs in monocytes and in vitro differentiated M2 Mφ identified seventeen differentially expressed lncRNAs. Based on fold-change and significance, we selected four differentially expressed lncRNAs viz., RN7SK, GAS5, IPW, and ZFAS1 to evaluate their functional impact. LncRNA knockdown was performed on day 3 M2 Mφ and the impact on polarization was assessed on day 7 by surface marker analysis. Knockdown of RN7SK and GAS5 showed downregulation of M2 surf...
Source: Frontiers in Immunology - December 9, 2020 Category: Allergy & Immunology Source Type: research

Cloning short DNA into plasmids by one ‐step PCR
ConclusionsFree from the limitations of restriction enzyme sites and omitting the ligation process, our method offers a flexible and economical option of plasmid construction.Key pointsSignificant findings of the studyA method to clone short DNA into plasmids was found.What this study addsOur study provides a flexible and economical option to clone short DNA into plasmids.
Source: Thoracic Cancer - October 4, 2020 Category: Cancer & Oncology Authors: Cheng ‐Cheng Tao, Ying Yang, Fang Li, Ling Qiao, Yue Wu, Xiao‐Dong Sun, Yuan‐Yuan Zhang, Chang‐Long Li Tags: TECHNOLOGICAL NOTE Source Type: research