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Total 216 results found since Jan 2013.

Silencing Estrogen Receptor-β with siRNA in Cultured Cells
Estrogen receptors α and β (ERα and ERβ) are the two genomic estrogen receptors. ERβ was the second of these receptors to be discovered; its structure is similar to that of ERα but they are different in histological distribution. However, the functions of ERα versus ERβ are still unclear. The ability of small interfering RNAs (siRNAs) to silence gene expression has proven to be invaluable for studying gene function in cultured mammalian cells. This chapter describes the use of siRNA to inhibit the expression of ERβ in renal cell carcinoma (RCC) and to further the understandi...
Source: Springer protocols feed by Biochemistry - October 12, 2015 Category: Biochemistry Source Type: news

Silencing Estrogen Receptor- & beta; with siRNA in Cultured Cells
Estrogen receptors & alpha; and & beta; (ER & alpha; and ER & beta;) are the two genomic estrogen receptors. ER & beta; was the second of these receptors to be discovered; its structure is similar to that of ER & alpha; but they are different in histological distribution. However, the functions of ER & alpha; versus ER & beta; are still unclear. The ability of small interfering RNAs (siRNAs) to silence gene expression has proven to be invaluable for studying gene function in cultured mammalian cells. This chapter describes the use of siRNA to inhibit the expression of ER & beta; in renal cell carcinoma (RCC) and to further...
Source: Springer protocols feed by Biochemistry - October 11, 2015 Category: Biochemistry Source Type: news

The effects of RNA interference mediated VEGF gene silencing on biological behavior of renal cell carcinoma and transplanted renal tumor in nude mice.
CONCLUSION: The VEGF gene silencing could inhibit the cell proliferation, migration and invasion of RCC 786-O cells; inhibition of VEGF protein expression could prevent transplanted RCC growth and tumor angiogenesis. PMID: 26484606 [PubMed - as supplied by publisher]
Source: Cancer Biomarkers : Section A of Disease Markers - September 30, 2015 Category: Cancer & Oncology Authors: Sun SQ, Cheng ZH, Wang S, Sun XW, Chen G, Zhang Y, Gu M, Yao HJ, Wang Z, Zhou J, Peng YB, Xu MX, Zhang K, Wang Q Tags: Cancer Biomark Source Type: research

MYSM-1 suppresses migration and invasion in renal carcinoma through inhibiting epithelial-mesenchymal transition
Abstract Renal cell carcinoma (RCC) is the most common malignant renal tumor and is prone to metastasis. However, the molecular variation and mechanism underlying renal cell carcinoma metastasis remains largely unknown. In our previous study, it was found that MYSM-1 was significantly downregulated in renal cell carcinoma tissues as compared with normal renal tissues without metastasis, using proteomics approach. Therefore, we hypothesized that MYSM-1 may suppress the metastasis of renal cell carcinoma in light of paucity of data regarding MYSM-1 in the cancers. In the present study, to confirm the expression stat...
Source: Tumor Biology - September 27, 2015 Category: Cancer & Oncology Source Type: research

siRNA Suppression of NEDD9 Inhibits Proliferation and Enhances Apoptosis in Renal Cell Carcinoma.
In this study, we demonstrate for the first time that NEDD9 was upregulated in RCC tissue and cell lines. Immunohistochemical analysis and quantitative RT-PCR analysis showed low expression of NEDD9 in normal renal tissues and high expression in RCC tissues. In addition, in vitro experiments show that expression of NEDD9 was upregulated in RCC cell lines. Through MTT assay, we observed that NEDD9 knockdown inhibited cell proliferation. Furthermore, flow cytometry analysis showed that NEDD9 downregulation induced apoptosis. Together, our data suggest that abnormal NEDD9 protein expression may be a marker for RCC, and NEDD9 ...
Source: Oncology Research - September 11, 2015 Category: Cancer & Oncology Tags: Oncol Res Source Type: research

C-Raf Colocalizes with DAPK in the Mitochondria
This study identified the interaction of C-Raf with S308 phosphorylated DAPK (pDAPKS308), which together became colocalized in the mitochondria to facilitate mitochondrial remodeling. Combined use of the Raf inhibitors sorafenib and GW5074 had synergistic anticancer effects in vitro and in vivo, but targeted mitochondrial function, rather than the canonical Raf signaling pathway. C-Raf depletion in knockout MEFC-Raf−/− or siRNA knockdown ACHN renal cancer cells abrogated the cytotoxicity of combination therapy. Crystal structure simulation showed that GW5074 bound to C-Raf and induced a C-Raf conformational change that...
Source: Cancer Research - August 31, 2015 Category: Cancer & Oncology Authors: Tsai, Y.-T., Chuang, M.-J., Tang, S.-H., Wu, S.-T., Chen, Y.-C., Sun, G.-H., Hsiao, P.-W., Huang, S.-M., Lee, H.-J., Yu, C.-P., Ho, J.-Y., Lin, H.-K., Chen, M.-R., Lin, C.-C., Chang, S.-Y., Lin, V. C., Yu, D.-S., Cha, T.-L. Tags: Therapeutics, Targets, and Chemical Biology Source Type: research

Impact of O6-methylguanine-DNA methyltransferase expression on the drug resistance of clear cell renal cell carcinoma
Conclusions The overexpression of O6-methylguanine-deoxyribonucleic acid methyltransferase contributes to resistance of clear cell renal cell carcinoma to standard chemotherapy. Our results have significance for understanding a new pathway of the development of drug resistance of clear cell renal cell carcinoma.
Source: Japanese Journal of Clinical Oncology - August 26, 2015 Category: Cancer & Oncology Authors: Ge, N., Guo, L., Zhang, J., Lin, Z., Li, Y., Liu, Y., Kong, F., Fang, X., Zhao, S. Tags: Urology, Original Articles Source Type: research

Abstract 4374: DCLK1 is a broadly dysregulated target against epithelial-mesenchymal transition, focal adhesion, and stemness in clear cell renal carcinoma
Renal clear cell carcinoma (RCC) is the most common type of kidney cancer and the 8th most common cancer overall in the US. RCC survival rates drop precipitously with regional and distant spread and recent studies have demonstrated that RCC presents an epithelial-mesenchymal transition (EMT) phenotype linked to increased recurrence and decreased survival. EMT is a key characteristic of tumor stem cells (TSCs) along with chemo-resistance and radio-resistance, which are also phenotypic of RCC.Targeting these factors is key to increasing the survival of RCC patients.Doublecortin-like kinase 1 (DCLK1) marks TSCs in pancreatic ...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Weygant, N., Qu, D., May, R., Tierney, R. M., Berry, W. L., Zhao, L., Agarwal, S., Chandrakesan, P., Sureban, S. M., Schlosser, M. J., Tomasek, J. J., Houchen, C. W. Tags: Experimental and Molecular Therapeutics Source Type: research

Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27
Conclusions: The data demonstrates that PHD3 can drive cell cycle entry at the G1/S transition through decreasing the half-life of p27 that occurs by attenuating p27S10 phosphorylation.
Source: Molecular Cancer - July 30, 2015 Category: Cancer & Oncology Authors: Heidi HögelPetra MiikkulainenLucia BinoPanu Jaakkola Source Type: research

Decreased expression of long non-coding RNA NBAT-1 is associated with poor prognosis in patients with clear cell renal cell carcinoma.
CONCLUSIONS: NBAT-1 is a novel molecular correlated with ccRCC progression; and it may represent a prognostic biomarker and therapeutic target in renal cancer diagnosis and treatment. PMID: 26097558 [PubMed - in process]
Source: International Journal of Clinical and Experimental Pathology - June 26, 2015 Category: Pathology Authors: Xue S, Li QW, Che JP, Guo Y, Yang FQ, Zheng JH Tags: Int J Clin Exp Pathol Source Type: research

PPAR{alpha} inhibition modulates multiple reprogrammed metabolic pathways in kidney cancer and attenuates tumor growth
Kidney cancer [renal cell carcinoma (RCC)] is the sixth-most-common cancer in the United States, and its incidence is increasing. The current progression-free survival for patients with advanced RCC rarely extends beyond 1–2 yr due to the development of therapeutic resistance. We previously identified peroxisome proliferator-activating receptor-α (PPARα) as a potential therapeutic target for this disease and showed that a specific PPARα antagonist, GW6471, induced apoptosis and cell cycle arrest at G0/G1 in RCC cell lines associated with attenuation of cell cycle regulatory proteins. We now extend t...
Source: AJP: Cell Physiology - June 1, 2015 Category: Cytology Authors: Abu Aboud, O., Donohoe, D., Bultman, S., Fitch, M., Riiff, T., Hellerstein, M., Weiss, R. H. Tags: ARTICLES Source Type: research

Ghrelin Promotes Renal Cell Carcinoma Metastasis via Snail Activation and Is Associated with Poor Prognosis
In this study, we observed that ghrelin was highly expressed in renal tumours, especially in metastatic RCC. In addition, high ghrelin levels correlated with poor outcome, lymph node and distant metastasis. The addition of ghrelin promoted the migration ability of RCC cell lines 786–0, ACHN and A‐498. Furthermore, knockdown of ghrelin expression reduced in vitro migration and in vivo metastasis, suggesting a requirement for ghrelin accumulation in the microenvironment for RCC metastasis. Analysis of microarray signatures using Ingenuity Pathway Analysis (IPA) and MetaCore pointed to the potential regulation by ghrelin ...
Source: The Journal of Pathology - April 29, 2015 Category: Pathology Authors: Tsung‐Chieh Lin, Yu‐Peng Liu, Yung‐Chieh Chan, Chia‐Yi Su, Yuan‐Feng Lin, Shih‐Lan Hsu, Chung‐Shi Yang, Michael Hsiao Tags: Original Paper Source Type: research

Ganglioside, Disialosyl Globopentaosylceramide (DSGb5), Enhances the Migration of Renal Cell Carcinoma Cells.
Abstract About one third of renal cell carcinoma (RCC) patients exhibit metastasis upon initial presentation. However, the molecular basis for RCC metastasis is not fully understood. A ganglioside, disialosyl globopentaosylceramide (DSGb5), was originally isolated from RCC tissue extracts, and its expression is correlated with RCC metastatic potential. DSGb5 is synthesized by GalNAc α2,6-sialyltransferase VI (ST6GalNAcVI) and is expressed on the surface of RCC cells. Importantly, DSGb5 binds to sialic acid-binding Ig-like lectin-7 (Siglec-7) expressed on natural killer (NK) cells, thereby inhibiting NK-cell cytot...
Source: The Tohoku Journal of Experimental Medicine - April 15, 2015 Category: Research Authors: Kawasaki Y, Ito A, Kakoi N, Shimada S, Itoh J, Mitsuzuka K, Arai Y Tags: Tohoku J Exp Med Source Type: research

Down-regulated long non-coding RNA H19 inhibits carcinogenesis of renal cell carcinoma.
Abstract Long non-coding RNAs (lncRNAs) have been shown to have important regulatory roles in cancer biology. LncRNA H19 has been recently shown to be upregulated and play important roles in several cancers such as breast cancer, bladder cancer, and gastric cancer. However, the role of H19 in clear cell renal carcinoma (ccRCC) remains largely unknown.The expression levels of lncRNA H19 in ccRCC tissues and renal cancer cell lines were evaluated by quantitative Real-time PCR (qRT-PCR). And its association with overall survival of patients was analyzed by statistical analysis. Small interfering RNA (siRNA) was used ...
Source: Neoplasma - April 13, 2015 Category: Cancer & Oncology Authors: Wang L, Jia X, Zhao X, Cai Y, Zhang J, Liu J, Zhen H, Wang T, Tang X, Liu Y, Wang J Tags: Neoplasma Source Type: research

miR-124 represses FZD5 to attenuate P-glycoprotein-mediated chemo-resistance in renal cell carcinoma
In conclusion, restoring miR-124 may function as a promising strategy to overcome P-gp-mediated MDR by inhibiting FZD5/PKC signaling.
Source: Tumor Biology - April 11, 2015 Category: Cancer & Oncology Source Type: research