Abnormal expression of lncRNA UCA1 disturbed cell apoptosis through mediating mitochondrial dynamics in PDAC.
Abstract A great number of studies have shown the pivotal role of mitochondria in cancer progression and numerous studies indicated that lncRNAs are involved in tumor metabolism. However, the relationship between UCA1 and mitochondria in PDAC remains unclear. Here, we reported for the first time that UCA1-driven change in mitochondrial dynamics induced mitochondrial apoptotic pathway in PDAC. In this research, data mining revealed that upregulated UCA1 occurred in PDAC patients, which meant a high likelihood of a poor prognosis. Following, UCA1 silencing could notably decrease cell viability and induce cell apopto...
Source: Neoplasma - December 23, 2020 Category: Cancer & Oncology Authors: Teng B, Feng T, Li W, Wang Z Tags: Neoplasma Source Type: research

Risk factors of pancreatic cancer and their possible uses in diagnostics.
rna J Abstract Pancreatic cancer (PC) is a form of malignancy of increasing incidence and poor prognosis, with an average of less than 10% of patients surviving 5 years after being diagnosed. The main reason for this unfavorable situation is the long asymptomatic course of the disease, and the absence of a simple screening method, typically leading to the late discovery of the disease. The development of the malignancy from the initial carcinogenesis into invasive pancreatic carcinoma takes approximately 10 years. However, the progression of pancreatic cancer from early into advanced stages can be, according to th...
Source: Neoplasma - December 23, 2020 Category: Cancer & Oncology Authors: Lang J, Kunovský L, Kala Z, Trna J Tags: Neoplasma Source Type: research

Trim47 overexpression correlates with poor prognosis in gastric cancer.
Abstract Trim47 is a member of the tripartite motif (TRIM) family that participates in many pathophysiological processes. However, the expression pattern and biological functions of Trim47 in gastric cancer (GC) remain unclear. The present study aimed to further explore the clinicopathological significance and potential prognostic role of Trim47 expression in GC. Therefore, in this study, Trim47 mRNA level was investigated in the Cancer Genome Atlas (TCGA) and Oncomine database in GC. We detected Trim47 mRNA and protein expression levels in GC and paired adjacent normal tissues. Kaplan-Meier method and Cox proport...
Source: Neoplasma - December 23, 2020 Category: Cancer & Oncology Authors: Xia Y, Wei Z, Huang W, Wei X, He Y Tags: Neoplasma Source Type: research

Loss of neuropilin1 inhibits liver cancer stem cells population and blocks metastasis in hepatocellular carcinoma via epithelial-mesenchymal transition.
Abstract It is generally believed that the existence of cancer stem cells (CSCs) is related to tumor recurrence and metastasis of hepatocellular carcinoma (HCC). Neuropilin1 (NRP1) is involved in numerous pathophysiological processes of tumor progression, however, whether NRP1 is involved in the regulation of liver CSCs and metastasis of HCC is still unknown. In the present study, we examined the effect of NRP1 on the population of liver CSCs and the metastasis mechanism of HCC. In NRP1 small hairpin RNA (shRNA)-transduced HCC cells, liver CSCs surface markers (CD133+/ EpCAM+/CD13+/CD44+) expressing cells, which i...
Source: Neoplasma - December 23, 2020 Category: Cancer & Oncology Authors: Li X, Zhou Y, Hu J, Bai Z, Meng W, Zhang L, Song X, Wei Y, Yan J, Zhou Y Tags: Neoplasma Source Type: research

PGC-1 α activates SIRT3 to modulate cell proliferation and glycolytic metabolism in breast cancer.
In this study, we explored the roles of PGC-1α and SIRT3 in cell proliferation and mitochondrial energy metabolism alterations in breast cancer cells. The expression patterns of PGC-1α and SIRT3 were examined using qRT-PCR and western blotting analysis. MCF-7 and MDA-MB-231 cells were infected by adenovirus to overexpress or knock down the expression of PGC-1α and SIRT3. Cell viability and apoptosis were analyzed by CCK-8 and flow cytometry, respectively. Hexokinase2, pyruvate kinase activities, as well as NAD+/NADH ratio and ATP concentration, were assessed by commercial kits. Glucose consumption was mea...
Source: Neoplasma - November 25, 2020 Category: Cancer & Oncology Authors: Zu Y, Chen XF, Li Q, Zhang ST, Si LN Tags: Neoplasma Source Type: research

Curcumin combined with low-intensity ultrasound suppresses the growth of glioma cells via inhibition of the AKT pathway.
Abstract Malignant glioma is the most lethal form of brain cancer, and effective therapeutic modalities remain unavailable to date. We aim to investigate whether low-dose curcumin combined with low-intensity ultrasound (LIUS) effectively suppresses the growth of glioma cells and elucidate the underlying mechanisms. Glioma cells were treated with LIUS and curcumin. Subsequently, the effects of LIUS and curcumin on glioma cells were determined by CCK-8 assay, EdU assay, and flow cytometry analysis, respectively. Western blot analysis was performed to examine the levels of apoptosis-associated proteins and the protei...
Source: Neoplasma - November 25, 2020 Category: Cancer & Oncology Authors: Shi G, Zhang Z, Fang Y, Bian D, Bai Z Tags: Neoplasma Source Type: research

Kinesin family member 23 (KIF23) contributes to the progression of bladder cancer cells in vitro and in vivo.
Abstract The aim of our study was to detect the expression of KIF23 in human bladder cancer tissues and to assess the potential role of KIF23 in bladder cancer progression. The expression of KIF23 and the correlation with bladder cancer patients were explored using the TCGA database. Additionally, IHC assays were also performed to detect KIF23 expression in 95 bladder cancer tissues and corresponding non-tumor tissues collected in our hospital. Colony formation, MTT, and flow cytometry (FCM) assays were performed to detect its effects on bladder cancer cell proliferation and apoptosis, respectively. An animal mode...
Source: Neoplasma - November 25, 2020 Category: Cancer & Oncology Authors: Yao DW, Song Q, He XZ Tags: Neoplasma Source Type: research

Diagnostic ipsilateral central neck dissection may reduce undertreatment of initially low-risk papillary thyroid cancer.
The objective of this single-center retrospective study was to identify risk factors, which could help to distinguish patients who would need additional treatment after surgery. A total of 268 patients (39 men, 229 women, median age 49 years) underwent surgery between 2007-2015, and fulfilled inclusion criteria: tumor size ≤ 20 mm, absence of metastatic lymph nodes (LN) in the lateral neck compartment (LC), and absence of local invasion. Total thyroidectomy was performed in 252 cases, in 221 cases with central neck compartment (CC) dissection. The outcome - a more aggressive disease - was defined as the presence of meta...
Source: Neoplasma - November 25, 2020 Category: Cancer & Oncology Authors: Králik R, Grigerová M, Takácsová E, Waczulíková I, Durdík Š Tags: Neoplasma Source Type: research

Cancer-associated fibroblasts support bone tropic metastasis by acting as coordinators between the tumor microenvironment and bone matrix in breast cancer.
Abstract Bone is a common site of metastasis for various types of cancer cells, including breast cancer, and the consequent skeleton-related events observed in patients are severe and often fatal. Currently, it is widely accepted that cancer-associated fibroblasts (CAFs) confer a metastasis-promoting property to breast cancer cells. Furthermore, clinical observations suggest that CAFs mediate the bone tropism of metastatic breast cancer cells. Therefore, a deeper understanding of the mechanism by which CAFs are involved in the bone-tropic metastasis of breast cancer can facilitate the study of the novel and effect...
Source: Neoplasma - November 25, 2020 Category: Cancer & Oncology Authors: Li H, Lin X, Yang D, Chen Z, Wang X, Re F, Wei J, Chen J Tags: Neoplasma Source Type: research

miR-1246 shuttling from fibroblasts promotes colorectal cancer cell migration.
This study also indicates that targeting miR-1246 or blocking its transport from CAFs to CRC cells might represent a novel therapeutic approach in CRC treatment. PMID: 33231089 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - November 25, 2020 Category: Cancer & Oncology Authors: Si G, Li S, Zheng Q, Zhu S, Zhou C Tags: Neoplasma Source Type: research

Exosomal transfer of circular RNA FBXW7 ameliorates the chemoresistance to oxaliplatin in colorectal cancer by sponging miR-18b-5p.
Abstract Circular RNA F-box and WD repeat domain containing 7 (Circ-FBXW7) has been revealed to involve in the tumorigenesis of colorectal cancer (CRC). Exosomes are critical mediators of intercellular communication. However, the role of exosomal circ-FBXW7 in the CRC oxaliplatin resistance remains unknown. Cell viability, apoptosis, motility, and drug efflux were measured by the cell counting kit-8 assay, flow cytometry, transwell assay, and atomic absorption spectrophotometry, respectively. The expression of circ-FBXW7 and microRNA (miR)-18b-5p was detected using the quantitative real-time polymerase chain react...
Source: Neoplasma - November 5, 2020 Category: Cancer & Oncology Authors: Xu Y, Qiu A, Peng F, Tan X, Wang J, Gong X Tags: Neoplasma Source Type: research

The mechanism of ABL1 upregulating the expression of PD-L1 and the therapeutic effect of PD-L1 and STAT3 inhibitors in lung adenocarcinoma.
Abstract The upregulation of programmed cell death-ligand 1 (PD-L1) and continuous mutation of EGFR could induce chemoresistance in somatic cancers, however, the molecular mechanism of oncogene ABL1 in regulating the expression of PD-L1 in lung adenocarcinoma (LAD) remains unclear. In addition, the therapeutic effect of STAT3 and PD-L1 inhibitors in LAD is not fully understood. The ABL1 lentiviruses were used to transfect LAD cell lines (H1975, PC-9) with different EGFR mutation subtypes. Next, the expression of the JAK/STAT3 and PD-L1 pathway was detected followed by the treatment with STAT3 and PD-L1 inhibitors....
Source: Neoplasma - November 5, 2020 Category: Cancer & Oncology Authors: Tang D, Jiang H, Li Z, Gao W, Sun Y Tags: Neoplasma Source Type: research

LncRNA MEG8 plays an oncogenic role in hepatocellular carcinoma progression through miR-367-3p/14-3-3 ζ/TGFβR1 axis.
In conclusion, this work exposed a novel role and regulatory mechanism of MEG8 in HCC and provided new insight into the treatment of HCC. PMID: 33147050 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - November 5, 2020 Category: Cancer & Oncology Authors: Lou J, Yan W, Li QY, Zhu AK, Tan BQ, Dong R, Zou XZ, Liu T Tags: Neoplasma Source Type: research

Expression of COX-2, p16, and Ki67 in the range from normal breast tissue to breast cancer.
The objective of the study was to examine COX-2, p16, and Ki67 expression in a broad spectrum of breast lesions in order to define the proteins' phenotype throughout the tumorigenesis. Expression was studied by immunohistochemistry in 308 human breast samples divided into 7 subgroups - flat epithelial atypia (FEA), atypical hyperplasia (ADH), intraductal carcinoma (DCIS), invasive cancer (IC), benign lesions (BLs), normal tissue adjacent to breast cancer (CANT), and fatty tissue (FT). Analysis among 4 subgroups - premalignant lesions (DIN), IC, BLs, and normal tissue was also performed. High prevalence of COX-2 overexpress...
Source: Neoplasma - November 5, 2020 Category: Cancer & Oncology Authors: Feriancová M, Walter I, Singer CF, Gazdarica J, Pohlodek K Tags: Neoplasma Source Type: research

Upregulation of microRNA-650 by PBX1 is correlated with the development of Helicobacter pylori-associated gastric carcinoma.
This study was designed to investigate the expression of miR-650, a well-recognized oncogenic miRNA in GC samples and to analyze the associations between this miRNA and HP infection and the molecular mechanism. Following miRNA- and mRNA-based microarray analyses, miR-650, pre-B-cell leukemia transcription factor 1 (PBX1), and LATS2 were filtered as targets. After that, function assays were implemented to assess their function in GC cells. miR-650 was upregulated in HP+ tissues and cells, and inhibition of miR-650 attenuated cell proliferation, invasion, migration, yet enhanced apoptosis. PBX1 was overexpressed in HP+ tissu...
Source: Neoplasma - November 5, 2020 Category: Cancer & Oncology Authors: Liu J, Wang L, Li J, Xu Y Tags: Neoplasma Source Type: research

Comparison of claudin-3 and claudin-4 expression in bilateral and unilateral breast cancer.
Abstract Claudins are essential components of tight junctions, which are frequently deregulated in breast cancer. The aim of the current study was to assess claudin-3 and -4 expression in bilateral breast cancer (BBC) and unilateral breast cancer (UBC). Immunohistochemical expression of claudin-3, claudin-4 was evaluated in tissue microarrays containing 174 cases of BBCs paired with 174 cases of solitary tumors. Each case was classified as claudin-high or claudin-low depending on the H-score value. The results were correlated with histopathological features and the expression of basic breast cancer biomarkers. Med...
Source: Neoplasma - November 5, 2020 Category: Cancer & Oncology Authors: Szade J, Kunc M, Pieczyńska-Uziębło B, Świerblewski M, Biernat W, Jassem J, Senkus E Tags: Neoplasma Source Type: research

Downregulation of CDC20 suppressed cell proliferation, induced apoptosis, triggered cell cycle arrest in osteosarcoma cells, and enhanced chemosensitivity to cisplatin.
In this study, we aim to explore the role of CDC20 in two independent human OS cell lines' biological phenotype and chemotherapy sensitivity. We applied multiple approaches to measure cell growth, cell cycle, and apoptosis with or without deregulation or overexpression of CDC20. We found that the downregulation of CDC20 by siRNA or apcin suppressed cell proliferation, induced apoptosis, and triggered cell cycle arrest. Consistently, overexpression of CDC20 in normal cells promoted cell growth, inhibited apoptosis. What's more, the additional treatment of siCDC20 or apcin achieved better anticancer effects than that of cisp...
Source: Neoplasma - October 30, 2020 Category: Cancer & Oncology Authors: Gao Y, Guo C, Fu S, Cheng Y, Song C Tags: Neoplasma Source Type: research

Establishment and validation of prognostic nomograms to predict overall survival and cancer-specific survival for patients with osteosarcoma.
This study aimed to develop and validate nomograms predicting the survival of osteosarcoma patients from the SEER database and our hospital. Data of 1066 osteosarcoma patients from the SEER database were randomly divided into a development cohort (n = 800) and validation cohort one (n = 266). Another cohort of 126 patients from our hospital was utilized as validation cohort two. Univariate and multivariate Cox analyses were performed to identify the independent prognostic factors for overall survival (OS) and cancer-specific survival (CSS). Nomograms predicting the 3- and 5-year OS and CSS probability were constructed and ...
Source: Neoplasma - October 30, 2020 Category: Cancer & Oncology Authors: Yang QK, Lai QY, Wang Y, Wang Y, Yao ZX, Zhang XJ Tags: Neoplasma Source Type: research

Plasma SGIP1 methylation in diagnosis and prognosis prediction in hepatocellular carcinoma.
This study aimed to investigate the diagnostic and prognostic value of plasma SGIP1 methylation in HCC. The study included a total of 269 subjects, in which 129 were with HCC, 45 with liver cirrhosis (LC), 45 with chronic hepatitis B (CHB), and 50 were healthy controls (HCs). The aberrant methylation was detected by quantitative methylation-specific polymerase chain reaction (qMSP). The area under the curve (AUC) was 0.872 in distinguishing HCC from HCs, with a sensitivity of 85.3% and a specificity of 88%. The AUC was 0.728, when it distinguished HCC from CHB, with a sensitivity of 43.4% and a specificity of 97.8%. The AU...
Source: Neoplasma - October 30, 2020 Category: Cancer & Oncology Authors: Xie GF, Xu YX, Xu F, Sun LY, Ye ZL, Ma JJ, Wang HY, Shao JY Tags: Neoplasma Source Type: research

LncRNA MEG3 promotes endoplasmic reticulum stress and suppresses proliferation and invasion of colorectal carcinoma cells through the MEG3/miR-103a-3p/PDHB ceRNA pathway.
Abstract LncRNA maternally expressed gene 3 (MEG3) is a potential prognostic and diagnostic biomarker in colorectal carcinoma (CC). However, its cellular functions and mechanism remain not fully uncovered. Relative expression of MEG3, miRNA (miR)-103a-3p, and pyruvate dehydrogenase E1 subunit beta (PDHB) was detected by RT-qPCR and western blotting. Cell proliferation was measured by CCK-8 assay, colony formation assay, and flow cytometry, as well as xenograft tumor assay. Transwell assay examined cell invasion. Endoplasmic reticulum (ER) stress was evaluated by western blotting. Dual-luciferase reporter assay and...
Source: Neoplasma - October 30, 2020 Category: Cancer & Oncology Authors: Wang G, Ye Q, Ning S, Yang Z, Chen Y, Zhang L, Huang Y, Xie F, Cheng X, Chi J, Lei Y, Guo R, Han J Tags: Neoplasma Source Type: research

Tumor suppressor miR-424-5p abrogates ferroptosis in ovarian cancer through targeting ACSL4.
Abstract Ovarian cancer is the most lethal gynecological cancer. In spite of recent advances, clinical outcomes remain poor, urgently needing novel therapeutic approaches. Growing evidence indicates that microRNAs play crucial roles in ovarian carcinogenesis and progression and that ferroptosis serves as a novel tumor suppressor. However, the molecular mechanisms of miRNA-mediated ferroptosis regulation in ovarian cancer are still largely unknown. In the present study, we show that miR-424-5p negatively regulates ferroptosis by directly targeting ACSL4 in ovarian cancer cells. Upregulation of miR-424-5p suppressed...
Source: Neoplasma - October 8, 2020 Category: Cancer & Oncology Authors: Ma LL, Liang L, Zhou D, Wang SW Tags: Neoplasma Source Type: research

Loss of tumor intrinsic PD-L1 confers resistance to drug-induced apoptosis in human colon cancer.
Abstract Colorectal cancer (CRC) with BRAF (V600E) is associated with microsatellite instability (MSI) that predicts response to immune checkpoint inhibitors. We demonstrated the interrogation of TCGA RNA-seq human datasets revealed that BRAFV600E tumors had significantly higher Programmed Death Ligand 1 (PD-L1) mRNA compared to non-mutated BRAF CRCs. Also, MSI-H tumors were evaluated as higher PD-L1 than MSS CRCs. Inhibition of MEK/ERK by cobimetinib or CDK inhibitor dinaciclib was shown to attenuate mutant BRAF-induced PD-L1 coincident with reduced c-JUN and YAP expression whose combined knockdown reduced PD-L1....
Source: Neoplasma - October 8, 2020 Category: Cancer & Oncology Authors: Feng D, Chen Z, He X, Huang S, Zhang Z Tags: Neoplasma Source Type: research

HMGB1-activated fibroblasts promote breast cancer cells metastasis via RAGE/aerobic glycolysis.
Abstract Highly expressed high mobility group box-1 protein (HMGB1) promotes tumor metastasis. Whether HMGB1 participates in breast cancer cell activation of fibroblasts is unknown. The culture medium of 6 breast cancer cell lines with different migration potential, and with HMGB1 overexpression or knockdown was used to induce fibroblast activation, and collagen and α-SMA expression were measured. We evaluated the migration potential of MDA-MB-231 cells with fibroblasts treated with 3-PO (3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) inhibitor, anti-HMGB1 treatment, or RAGE (receptor for advanced glycation...
Source: Neoplasma - October 8, 2020 Category: Cancer & Oncology Authors: Chen Y, Cai L, Guo X, Li Z, Liao X, Zhang X, Huang L, He J Tags: Neoplasma Source Type: research

Partial prostate re-irradiation for the treatment of isolated local recurrence of prostate cancer in patients previously treated with primary external beam radiotherapy: short-term results of a monocentric study.
Abstract Many different therapeutic options are available for locally recurrent prostate cancer (PCa). However, standard treatment has not yet been established. We conducted a partial prostate re-irradiation (PPR) program for the treatment of isolated and limited-size intraprostatic recurrences, in patients who previously underwent external beam radiation therapy (EBRT) as primary treatment for prostatic cancer (PCa). The analysis of this experience in terms of feasibility, toxicity, and efficacy is reported. The inclusion criteria of this retrospective analysis were: previous definitive EBRT, evidence of biochemi...
Source: Neoplasma - October 8, 2020 Category: Cancer & Oncology Authors: Matrone F, Revelant A, Fanetti G, Polesel J, Chiovati P, Avanzo M, De Renzi F, Colombo CB, Arcicasa M, De Paoli A, Franchin G, Bortolus R Tags: Neoplasma Source Type: research

Tumor suppressor miR-424-5p abrogates ferroptosis in ovarian cancer through targeting ACSL4.
Abstract Ovarian cancer is the most lethal gynecological cancer. In spite of recent advances, clinical outcomes remain poor, urgently needing novel therapeutic approaches. Growing evidence indicates that microRNAs play crucial roles in ovarian carcinogenesis and progression and that ferroptosis serves as a novel tumor suppressor. However, the molecular mechanisms of miRNA-mediated ferroptosis regulation in ovarian cancer are still largely unknown. In the present study, we show that miR-424-5p negatively regulates ferroptosis by directly targeting ACSL4 in ovarian cancer cells. Upregulation of miR-424-5p suppressed...
Source: Neoplasma - October 7, 2020 Category: Cancer & Oncology Authors: Ma LL, Liang L, Zhou D, Wang SW Tags: Neoplasma Source Type: research

Homeobox C4 promotes hepatocellular carcinoma progression by the transactivation of Snail.
Abstract Homeobox C4 (HOXC4) belongs to the homeoprotein family of transcription factors, which play a critical role in morphogenesis and differentiation during embryonic development. Aberrant expression of HOXC4 has been reported in several types of cancers. However, the role of HOXC4 in hepatocellular carcinoma (HCC) remains unknown. Here, we reported that HOXC4 is upregulated in HCC tissues and predicts a poor outcome in patients with HCC. HOXC4 promotes HCC progression and induces an EMT-like phenotype both in vitro and in vivo. Furthermore, we demonstrated that the EMT-related transcription factor Snail is a ...
Source: Neoplasma - September 24, 2020 Category: Cancer & Oncology Authors: Yang T, Zhang XB, Li XN, Sun MZ, Gao PZ Tags: Neoplasma Source Type: research

Differential profiling of prostate tumors versus benign prostatic tissues by using a 2DE-MALDI-TOF-based proteomic approach.
aron; M, Lichardusová L, Kaplán P Abstract Oncoproteomic technologies offer a complementary approach to the understanding of cancer proteins' function and the translation of molecular knowledge into clinical practice. Our aim was to compare the proteomic profiles of prostate tumors versus benign prostatic hyperplasia (BPH) tissues in order to identify modulated proteins as the potential biomarkers for prostate cancer. Proteins extracted from twenty prostate cancer tissue specimens and ten BPH tissues were analyzed by two-dimensional electrophoresis (2-DE) coupled with MALDI-TOF mass spectrometry. Wes...
Source: Neoplasma - September 24, 2020 Category: Cancer & Oncology Authors: Kmeťová Sivoňová M, Tatarková Z, Jurečeková J, Kliment J, Híveš M, Lichardusová L, Kaplán P Tags: Neoplasma Source Type: research

Potassium voltage-gated channel subfamily D member 2 induces an aggressive phenotype in lung adenocarcinoma.
Abstract The study aimed to investigate the roles of potassium voltage-gated channel subfamily D member 2 (KCND2) in lung adenocarcinoma (AD). RNA sequencing data from The Cancer Genome Atlas (TCGA) database showed that the expression of KCND2 was elevated in lung AD samples compared to the normal samples, and its upregulation was significantly associated with the unfavorable clinic outcome of lung AD patients. Cell proliferation and transwell assays revealed that the growth, migration, and invasion of lung AD cells, which was crucial to cancer aggressiveness, were markedly inhibited after the depletion of KCND2. ...
Source: Neoplasma - September 24, 2020 Category: Cancer & Oncology Authors: Lu X, Li K, Yang J Tags: Neoplasma Source Type: research

Comparative molecular profiling of distant metastatic and non-distant metastatic lung adenocarcinoma.
Abstract Most lung cancer deaths are caused by a distant disseminated disease rather than primary tumors. Understanding the biology behind distant metastasis (DM) is crucial for the effective prediction and reduction of recurrence rates. Genome-wide analysis of the tumor provides a new way to explore the pathogenesis and molecular diagnosis of metastasis in lung adenocarcinoma. In our study, a total of 215 eligible lung adenocarcinoma patients were enrolled. The DNA was extracted from Formalin-fixed paraffin-embedded (FFPE) samples from the primary tumors of these patients. Comprehensive molecular profiling was pe...
Source: Neoplasma - September 16, 2020 Category: Cancer & Oncology Authors: Chen Z, Chen G, Wang Z, Yu J, Zhang H, Mao B, Ma H Tags: Neoplasma Source Type: research

Recognition of the organ-specific mutations in metastatic breast cancer by circulating tumor cells isolated in vivo.
Abstract The failure to treat and control the growth of metastases is the main cause of death in breast cancer (BC) patients. Compared to the traditional method of analyzing circulating tumor DNA (ctDNA), capturing intact circulating tumor cells (CTCs) allows us to more accurately characterize mutations and identify suitable targeted therapies. We used CellCollector to collect peripheral CTCs. Thirty metastatic breast cancer (MBC) patients were enrolled, and 17 were analyzed with next-generation sequencing (NGS) methods. Clinical characteristics were analyzed along with the CTCs enumeration and detection rates. Wh...
Source: Neoplasma - September 16, 2020 Category: Cancer & Oncology Authors: Li S, Yang S, Shi J, Ding Y, Gao W, Cheng M, Sun Y, Xie Y, Sang M, Yang H, Geng C Tags: Neoplasma Source Type: research

Circ_0086720 knockdown strengthens the radiosensitivity of non-small cell lung cancer via mediating the miR-375/SPIN1 axis.
Abstract Radioresistance is an important cause of cancer treatment failure. Circular RNAs (circRNAs) play crucial roles in cancer development, including the radioresistance. This research aimed to determine the function and related mechanism of circ_0086720 in the radioresistance of non-small cell lung cancer (NSCLC). The expression of circ_0086720, miR-375, and Spindlin 1 (SPIN1) was measured using a quantitative real-time polymerase chain reaction (qRT-PCR). Cell survival fraction was analyzed using colony formation assay, and cell apoptosis was monitored by flow cytometry assay. The activities of caspase3 and c...
Source: Neoplasma - September 16, 2020 Category: Cancer & Oncology Authors: Jin Y, Su Z, Sheng H, Li K, Yang B, Li S Tags: Neoplasma Source Type: research

Long-term clinical benefit of Peg-IFN α and NAs sequential anti-viral therapy on HBV related HCC.
Long-term clinical benefit of Peg-IFNα and NAs sequential anti-viral therapy on HBV related HCC. Neoplasma. 2020 Sep 17;: Authors: Qi WQ, Zhang Q, Wang X, Xu Y, Zhao P, Guo HH, Zhou CY, Sun Y, Liu L, Wang JB Abstract Analysis of the value of long-term anti-viral therapy using sequential Peg-IFN therapy and nucleos(t)ide analogues (NAs) improves the prognosis of HBV-related HCC. HBV-related HCC patients were classified into sequential therapy with Peg-IFNα-2a and NAs, and NAs therapy alone. All patients were followed up for 5 years. The survival rate, HCC recurrence rate, Child Pugh score, ...
Source: Neoplasma - September 16, 2020 Category: Cancer & Oncology Authors: Qi WQ, Zhang Q, Wang X, Xu Y, Zhao P, Guo HH, Zhou CY, Sun Y, Liu L, Wang JB Tags: Neoplasma Source Type: research

FANCD2 knockdown with shRNA interference enhances the ionizing radiation sensitivity of nasopharyngeal carcinoma CNE-2 cells.
This study provides a promising target for NPC radiotherapy. PMID: 32940045 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - September 16, 2020 Category: Cancer & Oncology Authors: Bao Y, Feng H, Zhao F, Zhang L, Xu S, Zhang C, Zhao C, Qin G Tags: Neoplasma Source Type: research

Real-world safety and efficacy of nivolumab for ≥ 2nd line treatment of metastatic renal cell carcinoma: A retrospective cohort study in Croatia, Hungary, and Malta.
The objective of our study was to assess the real-world safety and efficacy of nivolumab in the second- or later-line treatment of metastatic renal cell carcinoma (mRCC). We conducted a multicenter, retrospective, observational study of real-world data from patients who were treated with nivolumab under a patient expanded access program from 2015 to 2017 in Croatia, Hungary, and Malta. The primary safety endpoint was the discontinuation of therapy because of adverse events. The primary efficacy endpoint was overall survival (OS). We collected data from 87 patients with a median (interquartile range (IQR)) age of 63 (57-68)...
Source: Neoplasma - September 16, 2020 Category: Cancer & Oncology Authors: Vrdoljak E, Magri C, Gamulin M, Bošković L, Omrčen T, Bajić Ž, Dienes T, Geczi L Tags: Neoplasma Source Type: research

The potentially therapeutic targets of pediatric anaplastic ependymoma by transcriptome profiling.
This study provides global gene information and some new potential biomarkers for the diagnosis and therapeutic targets of ependymoma. PMID: 32940047 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - September 16, 2020 Category: Cancer & Oncology Authors: Wang J, Sun C, Liu M, Zang D, Wang C, Liu Q, Liu Y, Chen Q Tags: Neoplasma Source Type: research

The impact of 18F-FDG PET/CT in the clinical management of patients with lymph node metastasis of unknown primary origin.
Abstract The aim of this study was to evaluate the diagnostic performance and the utility of F-18-fluorodeoxyglucose (FDG) positron emission tomography-computed tomography (PET/CT) in the clinical management of patients presenting with lymph node metastasis of undefined primary origin (CUP). A total of 53 patients (34 males, 19 females) with a diagnosis of lymph node metastasis according to the histopathology and/or conventional imaging were enrolled in this retrospective study. Patients were divided into four groups according to the initial location of their metastasis - group 1, cervical lymph nodes (n = 39), gr...
Source: Neoplasma - September 3, 2020 Category: Cancer & Oncology Authors: Nikolova PN, Hadzhiyska VH, Mladenov KB, Ilcheva MG, Veneva S, Grudeva VV, Dineva SE, Asenov YN Tags: Neoplasma Source Type: research

Combination of metformin and cold atmospheric plasma induces glioma cell death to associate with c-Fos.
Abstract Glioma is the most common type of brain cancer. Chemotherapy combination with surgery and radiotherapy is a standard treatment for patients. Although there are many advances in glioma therapy, the prognosis of glioma patients has not significantly been improved over the past decades. Hence, there is still an urgent need to develop a new therapy to treat glioma. Cell viability was assessed by CellTiter Blue assay; flow cytometry (FCM) was used for detecting cell apoptosis; ROS detection was detected by ROS Assay; H2O2 detection was performed by hydrogen peroxide detection kits; real-time PCR and WB were us...
Source: Neoplasma - September 3, 2020 Category: Cancer & Oncology Authors: Yang F, Zhou Y, Yu H, Yang J, Zhu C, Ahmad N, Meng X, Zhao R, Zhuang J, Sun M Tags: Neoplasma Source Type: research

Current view of neoadjuvant chemotherapy in primarily resectable pancreatic adenocarcinoma.
acute; I, Blažková M, Slabý O, Mayer J Abstract Pancreatic ductal adenocarcinoma (PDAC) is now the 11th most common cancer and in 2018 there were 458,918 new cases worldwide. In the Czech Republic, a total of 2,173 patients were diagnosed in 2015, ranking the second in incidence worldwide. In contrast to other malignancies, recent research has not brought any major breakthrough in the treatment of PDAC and hence the prognosis remains very serious. Radical resection is the only curative approach, but after the initiation of the standard pathological evaluation of the resected tissue, according to the...
Source: Neoplasma - September 3, 2020 Category: Cancer & Oncology Authors: Eid M, Ostřížková L, Kunovský L, Brančíková D, Kala Z, Hlavsa J, Janeček P, Kosíková I, Blažková M, Slabý O, Mayer J Tags: Neoplasma Source Type: research

CacyBP/SIP protein reduces p53 stability by enhancing Mdm2 activity in p53 mutant glioma cells.
Abstract Our previous studies have illustrated that CacyBP/SIP (Calcyclin-binding protein or Siah-1-interacting protein) promoted the proliferation of glioma cells. However, the possible mechanism still needs to be clarified. In the current study, we aimed to uncover the potential mechanism of CacyBP/SIP in regulating glioma cell proliferation. We found that CacyBP/SIP decreased the protein level of p53, but not the mRNA level of p53 in p53 mutant U251 cell line, whereas, in p53 wild-type U87 cell line, CacyBP/SIP neither promoted its proliferation nor regulated the changes of p53 protein. Further investigation in...
Source: Neoplasma - September 3, 2020 Category: Cancer & Oncology Authors: Qian F, Tang T, Wang S, Wang B, Wang L, Shi H Tags: Neoplasma Source Type: research

Construction of CTC-ALK gene fusion detection system based on the multi-site magnetic separation in lung cancer and its clinical verification.
Abstract Detected in a variety of solid tumors, including lung cancer, the EML4-ALK fusion gene plays an important role in promoting the occurrence and development of cancer. The existing detection methods for EML4-ALK fusion gene are all targeted at surgical or post-sampling tumor tissues, which cannot achieve early detection and real-time monitoring; therefore, a minimally invasive ALK gene fusion detection system is explored and constructed. Vimentin, EpCAM, and EGFR antibodies were grafted, respectively, to prepare multi-site immunoliposome magnetic beads, so as to capture CTC in blood for RT-PCR detection, an...
Source: Neoplasma - August 27, 2020 Category: Cancer & Oncology Authors: Ma AP, Guo WX, Gao DH, Ke MY, Luo Q, Liu Q Tags: Neoplasma Source Type: research

Quantitative analysis of 2-hydroxyglutarate as a controversial oncometabolite in malignant gliomas.
The objective of this study was to confirm or to reject the hypothesis of the direct correlation of 2-HG concentration in tumor tissue and the results from IDH 1/IDH 2 point mutation analyses. The concentrations of 2-HG were quantified using high sensitive HPLC and Q-TOF HRMS spectrometer setup. Concurrently, the genetic mutation analyses of both IDH 1 (cytosolic) and IDH 2 (mitochondrial) were performed by the isolation of tumor tissue DNA, PCR amplification, and subsequent Sanger forward sequencing. Our results indicate that there is no definite correlation between the two as we identified cases of glioma tumors with sig...
Source: Neoplasma - August 27, 2020 Category: Cancer & Oncology Authors: Bystrický P, Kašubová I, Richterová R, Lasabová Z, Kolarovszki B Tags: Neoplasma Source Type: research

Significance of transcriptionally-active high-risk human papillomavirus in sinonasal squamous cell carcinoma: Case series and a meta-analysis.
al M, Skálová A, Mezencev R Abstract Sinonasal cancers represent a highly heterogeneous group of head and neck cancers, for which etiological and prognostic significance of high-risk human papillomavirus (HPV) infections has not yet been conclusively established. We investigated the presence of transcriptionally-active high-risk HPV in a series of 34 sinonasal squamous cell cancer (SNSCC) cases and evaluated the effect of transcriptionally-active HPV on the overall survival. In addition, we performed a meta-analysis of previously published studies, including this study, to summarize the prevalence of...
Source: Neoplasma - August 27, 2020 Category: Cancer & Oncology Authors: Švajdler M, Němcová J, Dubinský P, Metelková A, Švajdler P, Straka Ľ, Sakař R, Daum O, Michal M, Skálová A, Mezencev R Tags: Neoplasma Source Type: research

Cancer-associated fibroblasts promote cell proliferation and invasion via paracrine Wnt/IL1 β signaling pathway in human bladder cancer.
Cancer-associated fibroblasts promote cell proliferation and invasion via paracrine Wnt/IL1β signaling pathway in human bladder cancer. Neoplasma. 2020 Aug 28;: Authors: Yang F, Guo Z, He C, Qing L, Wang H, Wu J, Lu X Abstract Bladder cancer (BC) is the most common urinary system malignancy worldwide. However, the molecular mechanisms underlying its progression remain largely unexplored. Accumulating evidence indicates that the cancer-associated fibroblasts (CAFs), major constituents of tumor stroma, play a key role in tumor development. Herein, we have successfully isolated CAFs and paired norma...
Source: Neoplasma - August 27, 2020 Category: Cancer & Oncology Authors: Yang F, Guo Z, He C, Qing L, Wang H, Wu J, Lu X Tags: Neoplasma Source Type: research

miR-155-5p increases the sensitivity of liver cancer cells to adriamycin by regulating ATG5-mediated autophagy.
In conclusion, our findings showed that miR-155-5p could reverse ADR resistance in liver cancer by targeting ATG5, which may function as a potential target for liver cancer treatment. PMID: 32853020 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - August 27, 2020 Category: Cancer & Oncology Authors: Yu Q, Xu XP, Yin XM, Peng XQ Tags: Neoplasma Source Type: research

Association study of miR-22 and miR-335 expression levels and G2 assay related inherent radiosensitivity in peripheral blood of ductal carcinoma breast cancer patients.
This study was conducted on blood samples taken from 60 women diagnosed with Invasive Ductal Carcinoma (IDC) BC (mean age: 47 ± 9.93) and 30 healthy women (mean age: 44.43 ± 6.7). The standard G2 assay was performed to predict cellular radiosensitivity. To investigate miR-22 and miR-335 expression levels in peripheral blood mononuclear cells (PBMCs), qPCR was performed. The sensitivity and specificity of the mentioned miRNAs were assessed by plotting the Receiver Operating Characteristic (ROC) curve. Binary logistic regression was performed to identify the miRNA involvement in BC and cellular radiosensitivity...
Source: Neoplasma - August 27, 2020 Category: Cancer & Oncology Authors: Bakhtari N, Mozdarani H, Salimi M, Omranipour R Tags: Neoplasma Source Type: research

miR-877-5p antagonizes the promoting effect of SP on the gastric cancer progression.
Abstract Gastric cancer is one of the four major tumors in the world and the second leading cause of cancer-related death. It was reported that Substance P (SP), as an oncogenic factor, could regulate the expression of miRNAs in gastric cancer progression. Here, we focused on the role of miR-877-5p in gastric cancer development and the miR-877-5p involvement in the SP-mediated gastric cancer development. The mRNA expression level and cell proliferation were assessed by quantitative real-time PCR and cell counting kit-8 assay, respectively. Flow cytometry was conducted to detect apoptosis, followed by assessing the...
Source: Neoplasma - August 27, 2020 Category: Cancer & Oncology Authors: Guo XD, Zhang N, Sha L Tags: Neoplasma Source Type: research

Long non-coding RNA CASC2 enhances cisplatin sensitivity in oral squamous cell cancer cells by the miR-31-5p/KANK1 axis.
Abstract Oral squamous cell cancer (OSCC) is a primary malignant tumor of the head and neck. Long non-coding RNA cancer susceptibility candidate 2 (CASC2) is related to the chemoresistance of diverse tumors. At present, the resistance of OSCC to first-line chemotherapy drug cisplatin (DDP) is still a giant problem. Herein, we investigated the role and mechanism of CASC2 in OSCC resistance to DDP. Expression levels of CASC2, miR-31-5p, and KANK1 in OSCC tissues and cells were determined by qRT-PCR. The half-maximal inhibitory concentration (IC50) value of DDP-resistant OSCC cells and apoptosis of DDP-resistant OSCC...
Source: Neoplasma - August 13, 2020 Category: Cancer & Oncology Authors: Wang J, Jia J, Zhou L Tags: Neoplasma Source Type: research

Lamin B1 deficiency promotes malignancy and predicts poor prognosis in gastric cancer.
In conclusion, our studies indicate that lamin B1 deficiency is crucial in GC progression. Furthermore, the results elucidating the biological mechanisms of lamin B1 may potentially contribute to current GC treatment modalities. PMID: 32787434 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - August 13, 2020 Category: Cancer & Oncology Authors: Yu ZY, Jiang XY, Zhao RR, Luo CJ, Ren YX, Ma ZJ, Ye HL, Shi WG, Wang C, Jiao ZY Tags: Neoplasma Source Type: research

Association of quantitative MRI-based radiomic features with prognostic factors and recurrence rate in oropharyngeal squamous cell carcinoma.
ereczek-Fossa BA Abstract Radiomics focuses on extracting a large number of quantitative imaging features and testing both their correlation with clinical characteristics and their prognostic and predictive values. We propose a radiomic approach using magnetic resonance imaging (MRI) to decode tumor phenotype and local recurrence in oropharyngeal squamous cell carcinoma (OPSCC). The contrast-enhanced T1-weighted sequences from baseline MRI examinations of OPSCC patients treated between 2008 and 2016 were retrospectively selected. Radiomic features were extracted using the IBEX software, and hierarchical clustering...
Source: Neoplasma - August 13, 2020 Category: Cancer & Oncology Authors: Giannitto C, Marvaso G, Botta F, Raimondi S, Alterio D, Ciardo D, Volpe S, De Piano F, Ancona E, Tagliabue M, Origgi D, Chiocca S, Maffini FA, Ansarin M, Bagnardi V, Cattani F, Nole F, Preda L, Orecchia R, Cassano E, Cremonesi M, Starzynska A, Bellomi M, Tags: Neoplasma Source Type: research

Adjuvant radiotherapy treatment for soft tissue sarcoma of extremities and trunk. A retrospective mono-institutional analysis.
In conclusion, good local control and toxicity profile were observed, despite negative patients' selection at baseline. Further investigation on wider series is warranted in order to define the optimal combination with systemic therapy. PMID: 32787436 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - August 13, 2020 Category: Cancer & Oncology Authors: Spoto R, Vavassori A, Dicuonzo S, Pepa M, Volpe S, Alessandro O, Gandini S, Di Venosa B, Miglietta E, Fodor C, Orsolini GM, Prestianni P, Cattani F, Comi S, Lazzari R, Renne G, De Pas T, Orecchia R, Pennacchioli E, Jereczek-Fossa BA Tags: Neoplasma Source Type: research