Gastrointestinal stromal tumors and second primary malignancies: a retrospective monocentric analysis.
In conclusion, our study did not find any significant correlation between clinicopathological characteristics and the development of a second tumor in GIST patients. Further analyses and strict follow up protocols are needed in order to early diagnose and promptly treat a second primary tumor in the GIST population. PMID: 32657607 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - July 14, 2020 Category: Cancer & Oncology Authors: Comandini D, Grassi M, Prelaj A, Martelli V, Damiani A, Damiano G, Rebuzzi SE Tags: Neoplasma Source Type: research

Objective response of first-line chemotherapy of triple-negative breast cancer translates into survival benefit: an analysis in an independent, prospective clinical trial and a real-world setting.
This study sought to assess whether the objective response (OR, including complete response and partial response) of first-line chemotherapy can predict overall survival (OS) for patients with metastatic triple-negative breast cancer (mTNBC) in both clinical trial and a real-world setting. The survival predictable parameters were assessed in two independent cohorts, the training cohort of 236 patients as part of a phase 3 trial (CBCSG006, Trial registration number NCT0128762) and the validation cohort of 360 patients from the real-world setting. Univariable and multivariable Cox proportional hazard models were applied to e...
Source: Neoplasma - July 14, 2020 Category: Cancer & Oncology Authors: Li T, Zhu YH, Zhang J, Wang BY, Zhang S, Wang LP, Cao J, Tao ZH, Xie J, Hu XC Tags: Neoplasma Source Type: research

Total body irradiation is a crucial risk factor for developing secondary carcinomas after allogeneic hematopoietic stem cell transplantation in childhood.
Abstract Allogeneic hematopoietic stem cell transplantation (HSCT) has become a standard part of therapy for a variety of malignant and non-malignant disorders. With improved outcomes after HSCT, increasing attention has been drawn to late complications in long-term survivors. The development of secondary malignancies is recognized as one of the most serious complications. We have evaluated data in 426 patients (272 males, 154 females) who underwent allogeneic transplantation at median age of 7.9 years from 1989 till 2017 and were alive more than one year after transplantation for the occurrence of secondary solid...
Source: Neoplasma - July 14, 2020 Category: Cancer & Oncology Authors: Keslova P, Formankova R, Riha P, Sramkova L, Snajderova M, Malinova B, Luks A, Sterba J, Stary J, Sedlacek P Tags: Neoplasma Source Type: research

Etiology of candidemia in patients with solid tumors - 7 years of experience of one oncology center.
Abstract Although the candidemia still remains a serious health problem, the study of candidemia in cancer patients is limited. We conducted a retrospective analysis of candidemia among 31 adult patients hospitalized in one oncological center. The mean annual incidence of candidemia was 0.14 ± 0.05 / 1000 patient-days (range 0.04-0.91). In 10 patients (32.3%), the catheter-related infection was diagnosed; in the remaining 21 patients (67.7%), it was a secondary infection. From 31 patients of candidemia, 15 died (48.4% 30-day mortality), but an overall mortality rate during hospital stay was 61.3% (19 cases)...
Source: Neoplasma - July 14, 2020 Category: Cancer & Oncology Authors: Szymankiewicz M, Nowikiewicz T Tags: Neoplasma Source Type: research

Comparison of the efficacy and safety of the EC-T (epirubicin/cyclophosphamide followed by docetaxel) and TCb (docetaxel/carboplatin) neoadjuvant regimens in early TOP2A-normal stage II-III breast cancer.
This study aimed to compare the efficacy and safety of the EC-T (4 cycles of epirubicin 90 mg/m2 + cyclophosphamide 600 mg/m2, followed by 4 cycles of docetaxel 75 mg/m2) and TCb (6 cycles of docetaxel 75 mg/m2, intravenous drip (ID), day 1+carboplatin AUC 6, ID, day 1) neoadjuvant regimens in patients with TOP2A-normal stage II-III breast cancer. This study analyzed 280 patients enrolled from three studies registered with ClinicalTrials.gov (NCT03140553, NCT03154749, NCT03507465) with early TOP2A-normal stage II-III breast cancer who received neoadjuvant chemotherapy, including 100 patients who received the EC-T regimen a...
Source: Neoplasma - July 14, 2020 Category: Cancer & Oncology Authors: Li WP, Zhu T, Hu MX, Yang M, Ji F, Gao HF, Yang CQ, Zhang LL, Cheng MY, Xu FP, Wang K Tags: Neoplasma Source Type: research

Neuropilin1, a novel independent prognostic factor and therapeutic target in triple-negative breast cancer.
Abstract Triple-negative breast cancer (TNBC) is the most aggressive breast cancer, and thus, has limited treatment options. Neuropilin1 (NRP1) is a multi-functional transmembrane protein that interacts with a number of signaling receptors and plays an important role in cancer progression. Previous studies demonstrated that the expression of NRP1 is activated and promotes the progression of breast cancer particularly in TNBC compared to other molecular subtypes; however, whether or not the level of NRP1 expression is related to the progression of TNBC warrants further study. In the current study, we determined the...
Source: Neoplasma - July 14, 2020 Category: Cancer & Oncology Authors: Wang H, Huang J, Lv D, Zhang Y, Xu D, Shi X, Liu J, Ren H, Han Z Tags: Neoplasma Source Type: research

Third-line treatment for metastatic colorectal cancer: anlotinib is superior to chemotherapy and similar to fruquintinib or regorafenib.
Abstract The clinical efficiency and adverse reactions of anlotinib in metastatic colorectal cancer (mCRC) as a third-line treatment compared with chemotherapy and regorafenib or fruquintinib was explored in this study. Clinical data from 105 mCRC patients who failed at least two lines of chemotherapy were collected. The patients were divided into three groups based on their third-line therapeutic regimen: third-line chemotherapy only (group A); anlotinib (group B); and fruquintinib or regorafenib (group C). The result showed that the ORR and DCR of group B (14.29%, 85.71%) were higher than those of group A (0%, 4...
Source: Neoplasma - July 14, 2020 Category: Cancer & Oncology Authors: Cheng Y, Du FC, Fang FQ, Duan ZJ, Wei L, Shi KG Tags: Neoplasma Source Type: research

Correlation of survival length after pancreaticoduodenectomy for pancreatic head adenocarcinoma depending on tumor characteristics detected by means of computed tomography and resection margins status.
Abstract Pancreatic carcinoma is an aggressive tumor with a grim prognosis. Accurate staging is essential for indicating surgery in patients with borderline resectable tumors. This paper examines the correlation between pre-operation characteristics of tumors found on CT, infiltration of individual resection margins as confirmed by a pathologist, and the survival of patients with resectable pancreatic head ductal adenocarcinoma. This prospective cohort study involved patients operated on for pancreatic head adenocarcinoma, which was clearly resectable based on the staging CT and intraoperative observation between ...
Source: Neoplasma - July 2, 2020 Category: Cancer & Oncology Authors: Prochazka V, Hlavsa J, Kunovsky L, Farkasova M, Potrusil M, Andrasina T, Litavcova A, Mazanec J, Dolina J, Ostrizkova L, Eid M, Kovalcikova P, Pavlik T, Kala Z Tags: Neoplasma Source Type: research

Association of triple positivity with prognostic parameters and overall survival in a population-based study of 6,122 HER2-positive breast cancer patients: analysis of real-world clinical practice based on a research database.
Abstract Triple-positive breast cancer (TPBC), i.e. HER2-positive (HER2+) and hormone receptors-positive breast cancer, is a specific subgroup of breast cancers. TPBC biology is characterized by strong mutual interactions between signaling pathways stimulated by estrogens and HER2 amplification. The present study aims to carry out a population-based analysis of treatment outcomes in a cohort of hormone receptor (HR) positive and negative breast cancer patients who were treated with anti-HER2 therapy in the Czech Republic. The BREAST research database was used as the data source for this retrospective analysis. The...
Source: Neoplasma - July 2, 2020 Category: Cancer & Oncology Authors: Kolarova I, Vanasek J, Dolezel M, Stuk J, Hlavka A, Dusek L, Melichar B, Buchler T, Ryska A, Prausova J, Petrakova K, Tesarova P, Petera J, Vosmik M, Horackova K, Jarkovsky J Tags: Neoplasma Source Type: research

LncRNA SNHG4 promotes neuroblastoma proliferation, migration, and invasion by sponging miR-377-3p.
Abstract Long non-coding RNAs (lncRNAs) have been demonstrated to act as essential regulators in the growth and progression of neuroblastoma. In the present research, the high expression of lncRNA small nucleolar RNA host gene 4 (SNHG4) in neuroblastoma was tested via quantitative reverse transcription-polymerase chain reaction (qRT-PCR), and then the function of SNHG4 was explored and verified by CCK-8 assay, EdU assay, cell cycle assay, cell apoptosis test, wound healing test and invasion test in neuroblastoma cell lines. It was discovered that lncRNA SNHG4 exhibited high expression in neuroblastoma tissues and ...
Source: Neoplasma - July 2, 2020 Category: Cancer & Oncology Authors: Yang H, Guo JF, Zhang ML, Li AM Tags: Neoplasma Source Type: research

Plasma exosomal miR-125a-5p and miR-141-5p as non-invasive biomarkers for prostate cancer.
Abstract Predictive biomarkers for early diagnosis of prostate cancer are important for its treatment. The functional microRNAs in the exosomes of plasma and serum samples are of interest as stable and non-invasive biomarkers for recurrence in cancer patients. The present study aimed to clarify the value of plasma exosomal mRNA-125a-5p and miR-141-5p miRNAs as biomarkers for the diagnosis of prostate cancer. The study included 19 healthy individuals and 31 prostate cancer patients. In comparison to the levels in healthy controls, exosomal miR-141-5p levels showed a slight increase in prostate cancer patients (P = ...
Source: Neoplasma - July 2, 2020 Category: Cancer & Oncology Authors: Li W, Dong Y, Wang KJ, Deng Z, Zhang W, Shen HF Tags: Neoplasma Source Type: research

Improved survival in patients with FDG-PET/CT-based radiotherapy treatment planning for squamous cell anal cancer.
Abstract The aim of this retrospective analysis was to evaluate the impact of FDG-PET/CT-based target volume definition on locoregional control and survival, compared to conventional CT-based target volume definition and dose prescription. One hundred and twenty-two patients with squamous cell anal cancer were treated with curative radiotherapy (RT) alone (27%) or with RT with concurrent chemotherapy (73%) and analyzed. Forty-six percent had the early disease (stage I+II) and 54% were stage III. FDG-PET/CT-based staging was performed in 21% of the patients. The mean follow-up time was 60 months. Other risk factors...
Source: Neoplasma - July 2, 2020 Category: Cancer & Oncology Authors: Lohynska R, Mazana E, Novakova-Jiresova A, Jirkovska M, Nydlova A, Veselsky T, Malinova B, Buchler T, Stankusova H Tags: Neoplasma Source Type: research

miR-497 inhibits tumor growth and migration of osteosarcoma by targeting plexinA4 and CDK6.
Abstract MicroRNAs are small non-coding RNAs that regulate gene expression at the post-transcriptional level which have been reported to be involved in the pathogenesis of various cancers. In the present study, we found that miR-497 was downregulated in osteosarcoma tissues. Gain and loss of function studies were carried out to investigate the effect of miR-497 on the growth of osteosarcoma cells. The results indicated that miR-497 inhibited the growth of osteosarcoma cells. Furthermore, bioinformatics analysis predicted plexinA4 and CDK6 as targets of miR-497, which was afterward confirmed by luciferase activity ...
Source: Neoplasma - July 2, 2020 Category: Cancer & Oncology Authors: Tian ZS, Yan MJ, Li S, Cong D, Wang YY, Zhu QS Tags: Neoplasma Source Type: research

miR-15a-5p targets PHLPP2 in gastric cancer cells to modulate platinum resistance and is a suitable serum biomarker for oxaliplatin resistance.
In conclusion, miR-15a-5p was recognized as a biomarker for platinum resistance and prognosis in gastric cancer, interference of which may be a promising strategy for sensitizing gastric cancer to platinum drugs. PMID: 32567934 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - June 23, 2020 Category: Cancer & Oncology Authors: Pang K, Song J, Bai Z, Zhang Z Tags: Neoplasma Source Type: research

CircABCB10 silencing inhibits the cell ferroptosis and apoptosis by regulating the miR-326/CCL5 axis in rectal cancer.
Abstract Circular ATP binding cassette subfamily B member 10 (circABCB10) has been identified to have oncological functions in several tumors. However, the roles of circABCB10 in rectal cancer remain unknown. The expression of circABCB10, microRNA (miR)-326 and C-C motif chemokine ligand 5 (CCL5), and apoptosis related-protein was detected using quantitative real-time polymerase chain reaction or western blot, respectively. Cell survival or apoptosis was measured using cell counting kit-8 assay or flow cytometry. The accumulations of intracellular lipid reactive oxygen species (ROS) and Fe2+ were analyzed using C1...
Source: Neoplasma - June 23, 2020 Category: Cancer & Oncology Authors: Xian ZY, Hu B, Wang T, Cai JL, Zeng JY, Zou Q, Zhu PX Tags: Neoplasma Source Type: research

Diagnostic relevance of 18F-FDG PET/CT in newly diagnosed patients with monoclonal gammopathy of undetermined significance (MGUS): Single-center experience.
Abstract Monoclonal gammopathy of undetermined significance (MGUS) is a known precursor of more serious cancers, such as multiple myeloma (MM), Waldenström macroglobulinemia (MW) and other lymphoproliferative disorders. Using 18F-FDG PET/CT, we aimed to evaluate its benefit in early detection of various accompanying disorders and illnesses in MGUS patients. We prospectively analyzed the diagnostic relevance of 18F-FDG PET/CT in 390 newly diagnosed MGUS patients. On 18F-FDG PET/CT scans, the presence of focal or diffuse areas of detectable increased tracer uptake was recorded in 37 (9.5%) MGUS patients. The mo...
Source: Neoplasma - June 23, 2020 Category: Cancer & Oncology Authors: Sandecka V, Adam Z, Krejci M, Stork M, Rehak Z, Koukalova R, Sevcikova S, Brozova L, Kral Z, Mayer J, Pour L Tags: Neoplasma Source Type: research

Expression of selected microRNAs in pancreatic ductal adenocarcinoma: is there a relation to tumor morphology, progression and patient's outcome?
Abstract Pancreatic ductal adenocarcinoma (PDAC) remains a disease with extremely poor prognosis and limited effective available treatment. Differential expression of miRNAs isolated from tumor tissue has been proposed as a marker for tumor diagnosis, progression and prognosis. Nevertheless, the prognostic value of miRNAs expression in PDACs for patient outcome still remains unclear. Expression of 7 selected miRNAs, isolated from FFPE samples of 54 PDAC patients, was quantified using RT-qPCR. The relationship of miRNA expression levels with tumor histology, clinico-pathological characteristics, patient overall sur...
Source: Neoplasma - June 23, 2020 Category: Cancer & Oncology Authors: Szabo A, Gurlich R, Liberko M, Soumarova R, Vernerova Z, Mandys V, Popov A Tags: Neoplasma Source Type: research

miR-485-5p improves the progression of ovarian cancer by targeting SRC in vitro and in vivo.
Abstract miR-485-5p is involved in many tumors, but its role in ovarian cancer has been rarely reported. This paper mainly studied the expression and mechanism of miR-485-5p in ovarian cancer. The expression of miR-485-5p in ovarian cancer was compared with that in adjacent tissues, and the expression of miR-485-5p in various ovarian cancer cell lines was detected by RT-PCR. miR-485-5p mimics and pcDNA plasmid or pcDNA-SRC were transfected into SKOV3 cells. Cell proliferation was detected by CCK-8, cell cycle and apoptosis were detected by flow cytometry, and cell migration and invasion were detected by wound assa...
Source: Neoplasma - June 16, 2020 Category: Cancer & Oncology Authors: Yang Y, Liu J, Qian X, Li Y, Wang Y, Xu X Tags: Neoplasma Source Type: research

Long non-coding RNA GAS5 promotes natural killer cell cytotoxicity against gastric cancer by regulating miR-18a.
In conclusion, GAS5 promotes the killing effect of the natural killer cells against GC by regulating miR-18a, providing promising strategies for NK cells based antitumor therapies. PMID: 32538667 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - June 16, 2020 Category: Cancer & Oncology Authors: Wei MF, Gu ZS, Zheng LL, Zhao MX, Wang XJ Tags: Neoplasma Source Type: research

The knockdown of SNHG3 inhibits the progression of laryngeal squamous cell carcinoma by miR-340-5p/YAP1 axis and Wnt/ β-catenin pathway.
In conclusion, knockdown of SNHG3 inhibited LSCC progression via inactivating Wnt/β-catenin pathway by regulating the miR-340-5p/YAP1 axis. PMID: 32538668 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - June 16, 2020 Category: Cancer & Oncology Authors: Kang R, Yao DF, Xu GZ, Zhou YH Tags: Neoplasma Source Type: research

Evaluation of the prognostic value of fibrinolytic elements in invasive breast carcinoma patients.
Abstract Breast cancer (BrC) is one of the most serious oncological problems in the world. The aim of the study was to evaluate concentrations of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor type 1 (PAI-1) and their ratios: t-PA/PAI-1 and PAI-1/t-PA in breast cancer patients and in healthy individuals and to estimate the ability of fibrinolytic parameters in predicting neoplasm disease and disease relapse. One hundred and five women were enrolled in the study, including 60 cases with primary BrC, (M0) and 45 healthy females. Follow-up was completed in all BrC patients with a 16.7% recurr...
Source: Neoplasma - June 16, 2020 Category: Cancer & Oncology Authors: Ruszkowska-Ciastek B, Kwiatkowska K, Bielawska S, Robakowska M, Bielawski K, Rhone P Tags: Neoplasma Source Type: research

TSN inhibits cell proliferation, migration, invasion and EMT through regulating miR-874/HMGB2/ β-catenin pathway in gastric cancer.
In this study, we investigated the microRNA (miRNA) mediated gene repression mechanism in TSN-administrated GC condition. The cell viability of GC was determined by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Cell migration and invasion were detected by transwell assays. The expression levels of epithelial-mesenchymal transition (EMT)-associated proteins (N-cadherin, vimentin, E-cadherin), High-mobility group box proteins 2 (HMGB2), β-catenin pathway-related proteins (β-catenin, c-myc, cyclin D1) were detected by western blot analysis in TSN/GC. The expression patterns of miR-87...
Source: Neoplasma - June 2, 2020 Category: Cancer & Oncology Authors: Yuan F, Zhao ZT, Jia B, Wang YP, Lei W Tags: Neoplasma Source Type: research

KLHL22 promotes malignant melanoma growth in vitro and in vivo by activating the PI3K/Akt/mTOR signaling pathway.
Abstract The kelch like family member 22 (KLHL22) is a member of the KLHL (Kelch-like) gene family, which was involved in the progression of breast cancer. However, its role remains unclear in malignant melanoma (MM). Our study found that KLHL22 expression was upregulated in human MM tissues. Regarding the functional analysis for KLHL22 in the progression of MM cells, we demonstrated that overexpression of KLHL22 could promote MM cell growth in vitro. Vice versa, knockdown of KLHL22 could suppress the proliferation of MM cells. Furthermore, KLHL22 also promoted tumorigenesis of MM cells in vivo. In experiments inv...
Source: Neoplasma - June 2, 2020 Category: Cancer & Oncology Authors: Liu XR, Wang W, Li HM Tags: Neoplasma Source Type: research

miR-34a and miR-125a-5p inhibit proliferation and metastasis but induce apoptosis in hepatocellular carcinoma cells via repressing the MACC1-mediated PI3K/AKT/mTOR pathway.
Abstract microRNA-34a (miR-34a) and microRNA-1251-5p (miR-125a-5p) were considered as tumor suppressors in hepatocellular carcinoma (HCC). Nevertheless, the modulatory mechanisms of miR-34a and miR-125a-5p in HCC haven't been completely understood. The levels of metastasis-associated with colon cancer 1 (MACC1) and miRNAs (miR-34a and miR-125a-5p) were determined by quantitative real-time polymerase chain reaction (qRT-PCR), and the levels of associated proteins were detected by western blot assay. Cell proliferation and metastasis were examined via Cell Counting Kit-8 (CCK-8) and transwell assays, respectively. C...
Source: Neoplasma - June 2, 2020 Category: Cancer & Oncology Authors: Zhang YM, Wu QM, Chang LY, Liu JC Tags: Neoplasma Source Type: research

Death-inducer obliterator 1 (DIDO1) silencing suppresses growth of bladder cancer cells through decreasing SAPK/JNK signaling cascades.
Abstract Death inducer obliterator (DIDO) is involved in apoptosis and embryonic stem cell self-renewal. Here, we investigate the effect of DIDO1 on bladder cancer cells and clarify the underlying molecular mechanism. Bladder cancer tissues and cell lines (T24, ScaBER, 5637), as well as normal bladder epithelial cells (SV-HUC-1), were used to measure the levels of DIDO1 mRNA and protein by qRT-PCR and western blot, respectively. The results indicated that DIDO1 was highly expressed in bladder cancer tissues and cell lines. And the expression of DIDO1 in T24 and 5637 cells was higher than that in ScaBER and SV-HUC-...
Source: Neoplasma - June 2, 2020 Category: Cancer & Oncology Authors: Li J, Wang AS, Wang S, Wang CY, Xue S, Li WY, Ma TT, Shan YX Tags: Neoplasma Source Type: research

Knockdown of FBP1 enhances radiosensitivity in prostate cancer cells by activating autophagy.
In conclusion, this study clarified that FBP1 is an oncogene in prostate cancer, and the main mechanism for knockdown of FBP1 to increase radiosensitivity is to enhance autophagy mediated by the AMPK-mTOR signaling pathway. Therefore, FBP1 may be a potential target for enhancing prostate cancer radiotherapy. PMID: 32453596 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - May 26, 2020 Category: Cancer & Oncology Authors: Li XR, Zhou KQ, Yin Z, Gao YL, Yang X Tags: Neoplasma Source Type: research

Hypoxia-responsive miR-346 promotes proliferation, migration, and invasion of renal cell carcinoma cells via targeting NDRG2.
In this study, we investigated the role of miR-346 in RCC cells under hypoxia. OS-RC-2 and 786-O cells were cultured in 1% O2 or normal oxygen. Cell proliferation, migration, and invasion abilities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, transwell migration, and invasion assays, respectively. Quantitative real-time PCR (qRT-PCR) was performed to detect the expression of miR-346 and N-myc downstream-regulated gene 2 (NDRG2). Then bioinformatics analysis, dual-luciferase reporter assay, and RNA immunoprecipitation were carried out to determine the relationship between miR-3...
Source: Neoplasma - May 26, 2020 Category: Cancer & Oncology Authors: Su ZH, Liao HH, Lu KE, Chi Z, Qiu ZQ, Jiang JM, Wu Z Tags: Neoplasma Source Type: research

Circular RNA circ_0000615 knockdown suppresses the development of nasopharyngeal cancer through regulating the miR-338-3p/FGF2 axis.
In conclusion, our findings demonstrated that circ_0000615 knockdown suppressed the growth of NPC cells via modulating miR-338-3p/FGF2 axis, providing a theoretical basis for the treatment of NPC. PMID: 32453598 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - May 26, 2020 Category: Cancer & Oncology Authors: Liu HS, Zheng RN, Guo LB, Fu XJ Tags: Neoplasma Source Type: research

Norepinephrine release may play a critical role in the Warburg effect: an integrative model of tumorigenesis.
Abstract Many cancer cells share the property of carrying out markedly elevated rates of glycolysis to generate energy even in the presence of sufficient oxygen, and this is known as the Warburg effect. In recent years, there has been a resurgence of interest in the Warburg effect, as the field of oncology has amassed evidence that cellular metabolism may play a prominent role in many neoplasms. Largely in the past decade, another prominent and perhaps surprising factor has emerged in the cancer literature: the catecholamine molecules, epinephrine (adrenaline) and norepinephrine (noradrenaline), appear to play a r...
Source: Neoplasma - May 26, 2020 Category: Cancer & Oncology Authors: Fitzgerald PJ Tags: Neoplasma Source Type: research

miR-145-5p attenuates paclitaxel resistance and suppresses the progression in drug-resistant breast cancer cell lines.
In conclusion, our study suggested that miR-145-5p reduced PTX resistance and repressed the progression at least partly by targeting SOX2 in PTX-resistant BC cells, highlighting miR-145-5p as a promising biomarker for BC treatment. PMID: 32412771 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - May 15, 2020 Category: Cancer & Oncology Authors: Guan X, Guan Y Tags: Neoplasma Source Type: research

Clinical significance of perioperative EMT-CTC in rectal cancer patients receiving open/laparoscopic surgery.
The objective of this study was to explore the clinical significance of perioperative CTCs (circulating tumor cells) counts and EMT-CTCs (epithelial-mesenchymal transition-CTCs) in rectal cancer patients. A total of 30 patients with rectal cancer who underwent radical resection of rectal cancer at the Guangxi Zhuang Autonomous Region People's hospital were enrolled. Five ml peripheral blood was withdrawn from 30 patients with rectal cancer before the operation and seven days after the operation and at the corresponding time also from 20 healthy volunteers. CanPatrol™ CTC detection technique was used to enrich and ide...
Source: Neoplasma - May 15, 2020 Category: Cancer & Oncology Authors: Yin W, Han Y, Li ZL, Huang ZX, Huang L, Zhong XG Tags: Neoplasma Source Type: research

Decreased expression of GBA3 correlates with a poor prognosis in hepatocellular carcinoma patients.
Abstract Beta-glucosidase (GBA), also known as acid β-glucosidase, exhibits an activity of glucosylceramidase (EC 3.2.1.45). Three main isoforms of β-glucosidases have been identified in mammals: GBA1, GBA2, and GBA3. The deficiency of these enzymes leads to glucosylceramide accumulation, resulting in Gaucher's disease. The present study is focused on the cytosolic β-glucosidase, GBA3, and its relationship with hepatocellular carcinoma (HCC). The expression of GBA3 mRNA in HCC was evaluated first using the TCGA database, and then by immunohistochemistry using tissue microarrays of 328 clinically cha...
Source: Neoplasma - May 15, 2020 Category: Cancer & Oncology Authors: Ying JF, Zhang YN, Song SS, Hu ZM, He XL, Pan HY, Zhang CW, Wang HJ, Li WF, Mou XZ Tags: Neoplasma Source Type: research

Energy transfer from adipocytes to cancer cells in breast cancer.
In this study, we investigated the metabolic interaction between adipocytes and breast cancer cells and its potential as a new therapeutic target in breast cancer. Breast cancer cell lines and human breast cancer tissue samples were evaluated. Compared to cancer cells cultured alone, or the control group, those co-cultured with adipocytes showed lipid transfer from adipocytes to cancer cells and it was different according to the molecular subtype of breast cancer. Breast cancer cells affected the lipolysis of adipocytes and adipocytes affected the β-oxidation of breast cancer cells. The key molecule of the process was...
Source: Neoplasma - May 15, 2020 Category: Cancer & Oncology Authors: Kim HM, Lee YK, Kim ES, Koo JS Tags: Neoplasma Source Type: research

Regulation of HBV replication and gene expression by miR-501-3p via targeting ZEB2 in hepatocellular carcinoma.
Abstract Hepatitis B virus (HBV) infection is a major public health issue with serious medical consequences. The roles of microRNAs (miRNAs) in HBV replication and expression have been generally recognized, and the abnormal expression of miR-501 has been reported in patients with HBV infection. However, the function and mechanism in HBV replication remain elusive. The expression patterns of miR-501-3p and ZEB2 in HBV-related hepatocellular carcinoma (HCC) tissues and HBV-expressing HCC cells were determined by qRT-PCR and western blot assays. HBV replication and expression were evaluated through detecting the copi...
Source: Neoplasma - May 6, 2020 Category: Cancer & Oncology Authors: Zhong ZJ, Xu JF, Li ZZ, Zhou WY, Chen XX, Zhou JH, Li ZY Tags: Neoplasma Source Type: research

The inhibition of the breast cancer by PPAR γ agonist pioglitazone through JAK2/STAT3 pathway.
The inhibition of the breast cancer by PPARγ agonist pioglitazone through JAK2/STAT3 pathway. Neoplasma. 2020 May 06;: Authors: Jiao XX, Lin SY, Lian SX, Qiu YR, Li ZH, Chen ZH, Lu WQ, Zhang Y, Deng L, Jiang Y, Hu GH Abstract Breast cancer, especially triple negative breast cancer, is one of the deadliest cancers in women. To date, there is a lack of good therapeutics regime for it. PPARγ has been reported to be a tumor suppressor and could be activated by many agonists involved in cancer inhibition. Therefore, the expression of PPARγ in breast cancer was analyzed by online software ...
Source: Neoplasma - May 6, 2020 Category: Cancer & Oncology Authors: Jiao XX, Lin SY, Lian SX, Qiu YR, Li ZH, Chen ZH, Lu WQ, Zhang Y, Deng L, Jiang Y, Hu GH Tags: Neoplasma Source Type: research

Upregulation of SNHG12 accelerates cell proliferation, migration, invasion and restrain cell apoptosis in breast cancer by enhancing regulating SALL4 expression via sponging miR-15a-5p.
Abstract Breast cancer (BC) is malignant cancer that threatens the health of millions of females worldwide. Long non-coding RNA (lncRNA) small nucleolar RNA host gene 12 (SNHG12) has been identified as an oncogene in multiple cancers. However, the regulatory role of SNHG12 in BC cell progression is still obscured. The levels of SNHG12, miR-15a-5p, and Sal-like 4 (SALL4) in BC tumor tissues and cells were measured by qRT-PCR. Cell viability, apoptosis, migration and invasion were examined by CCK8, flow cytometry, and transwell assay, respectively. The interaction between miR-15a-5p and SNHG12 or SALL4 was evaluated...
Source: Neoplasma - May 6, 2020 Category: Cancer & Oncology Authors: Yuan JH, Li WX, Hu C, Zhang B Tags: Neoplasma Source Type: research

Study of DOK4 gene expression and promoter methylation in sporadic breast cancer.
Abstract The analysis of DNA methylation patterns in circulating cell-free DNA in body fluids is an analyte of great interest in clinical research. Downstream of Kinase (DOK) proteins represent a multigenic family of adaptors that includes negative regulators of immune cell signaling and plays important roles in signaling, cellular growth, and survival. The aim of the present study was to investigate the pattern of promoter methylation and expression of the DOK4 gene in breast cancer, and its possible association with histoclinical characteristics. The mRNA expression of the DOK4 gene was evaluated in 164 breast t...
Source: Neoplasma - May 6, 2020 Category: Cancer & Oncology Authors: Heidarizadi A, Salimi M, Mozdarani H Tags: Neoplasma Source Type: research

Kallikrein-related peptidase (KLK10) cessation blunts colorectal cancer cell growth and glucose metabolism by regulating the PI3K/Akt/mTOR pathway.
Abstract Colorectal cancer (CRC) is a common aggressive carcinoma with a proverbial feature of metabolic reprogramming that is essential for cancer cell growth. Recent research corroborates the controversial function of kallikrein-related peptidase 10 (KLK10) in cancer. However, its role and underlying mechanism in CRC remains elusive. In the present study, high expression of KLK10 was detected in CRC cell lines. Knockdown of KLK10 expression by a specific siRNA inhibited cell proliferation, evoked cell apoptosis, and increased caspase-3 activity in HT29 CRC cells. Furthermore, KLK10 suppression also afforded the ...
Source: Neoplasma - May 6, 2020 Category: Cancer & Oncology Authors: Wei H, Dong C, Shen Z Tags: Neoplasma Source Type: research

Hypomethylation of CTCFL promoters as a noninvasive biomarker in plasma from patients with hepatocellular carcinoma.
In conclusion, we provide evidence that the hypomethylation of CTCFL promoters in cell-free DNA is a biomarker for monitoring HCC patients, which can be used as a noninvasive prediction index for tumor recurrence and provide the individualized decision-making for clinicians. PMID: 32386482 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - May 6, 2020 Category: Cancer & Oncology Authors: Chen MM, Zhao RC, Chen KF, Huang Y, Liu ZJ, Wei YG, Jian Y, Sun AM, Qin L, Li B, Qin Y Tags: Neoplasma Source Type: research

LncRNA DSCAM-AS1 promotes non-small cell lung cancer progression via regulating miR-577/HMGB1 axis.
In conclusion, DSCAM-AS1 facilitated NSCLC progression by regulating the HMGB1-mediated Wnt/β-catenin pathway, providing a promising therapeutic target for NSCLC. PMID: 32386483 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - May 6, 2020 Category: Cancer & Oncology Authors: Qiu Z, Pan XX, You DY Tags: Neoplasma Source Type: research

Effects of sarcopenia, hypoalbuminemia, and laparoscopic surgery on postoperative complications in elderly patients with colorectal cancer: A prospective study.
The objective of this prospective study was to explore the effects of sarcopenia, hypoalbuminemia and laparoscopic surgery on postoperative complications among elderly patients who recently underwent colorectal surgery. Patients aged over 65 years who underwent surgery for CRC at the First Affiliated Hospital of Wenzhou Medical University were considered for this study. The demographical and clinical characteristics of these patients, as well as postoperative complications, were prospectively analyzed. The patients were divided into two groups depending on the diagnosis of sarcopenia and the clinical variables correspondin...
Source: Neoplasma - May 6, 2020 Category: Cancer & Oncology Authors: Chen WS, Huang YS, Xu LB, Shi MM, Chen XD, Ye GQ, Wu TT, Zhu GB Tags: Neoplasma Source Type: research

Effect of GATA4 gene methylation on proliferation and apoptosis of SGC-7901 gastric cancer cells.
In conclusion, restoration of GATA4 caused by 5-Aza treatment can inhibit the proliferation and promote apoptosis of GC cells possibly via Wnt/β-catenin signaling pathway. This study may offer new sight for GC treatment. PMID: 32305051 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - April 16, 2020 Category: Cancer & Oncology Authors: Ma X, Ji L Tags: Neoplasma Source Type: research

LncRNA CDKN2B-AS1 promotes the progression of ovarian cancer by miR-143-3p/SMAD3 axis and predicts a poor prognosis.
In conclusion, CDKN2B-AS1 promoted tumor growth, invasion, and migration of OC by regulation of miR-143-3p/SMAD3 axis, hinting that CDKN2B-AS1 might be a potential biomarker for OC diagnosis and treatment. PMID: 32305052 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - April 16, 2020 Category: Cancer & Oncology Authors: Xu C, Zhai J, Fu Y Tags: Neoplasma Source Type: research

LncRNA-LINC00261 suppresses the progression of NSCLC cells through upregulating miR-19a-mediated Kruppel-like factor 2 (KLF2).
Abstract Long non-coding RNA LINC00261 (LINC00261) has been reported to be implicated in tumorigenesis, treatment, and prognosis in different cancers including non-small cell lung cancer cells (NSCLCs). However, its mechanisms have been poorly investigated in NSCLC. Expressions of LINC00261, miR-19a and Kruppel-like factor 2 (KLF2) were detected using RT-qPCR and western blotting. Cell viability, migration and invasion and apoptosis were detected by MTT assay, transwell assay, flow cytometry and the expressions of Bcl-2, Bax, and cleaved caspase 3 were analyzed by western blotting. Tumor growth in vivo was measure...
Source: Neoplasma - April 16, 2020 Category: Cancer & Oncology Authors: Li ZY, Li ZZ, Zhou JH, Zhong ZJ, Wang XJ, Zhong L, Zhou WY Tags: Neoplasma Source Type: research

MiR-193a-5p suppresses cell proliferation and induces cell apoptosis by regulating HOXA7 in human ovarian cancer.
Abstract Ovarian cancer is one of the most common malignancies in women in the world. MicroRNAs (miRNAs) were identified as a group of regulators that played important roles in the progression of cancer development. The main purpose of this study was to investigate the functional mechanism of microRNA-193a-5p (miR-193a-5p) in human ovarian cancer. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the RNA levels of miR-193a-5p and homeobox genes A7 (HOXA7). Western blot assay was performed to determine the protein level of HOXA7. The interaction between miR-193a-5p and HOXA7 was predicte...
Source: Neoplasma - April 16, 2020 Category: Cancer & Oncology Authors: Wang S, Diao YJ, Zhu BB Tags: Neoplasma Source Type: research

LncRNA TUG1 promotes tumor growth and metastasis of esophageal squamous cell carcinoma by regulating XBP1 via competitively binding to miR-498.
Abstract Esophageal squamous cell carcinoma (ESCC) is a major subtype of esophageal cancer with high mortality. Previous reports suggested that lncRNA taurine up-regulated gene 1 (TUG1) functioned as an oncogene in numerous cancers. The purpose of this study was to explore the potential mechanism of TUG1 carcinogenesis in ESCC. The expression of TUG1 and miR-498 was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation and apoptosis were assessed by 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assay and flow cytometry. Cell migration and invasion we...
Source: Neoplasma - April 16, 2020 Category: Cancer & Oncology Authors: Jin G, Yang Y, Tuo G, Wang W, Zhu Z Tags: Neoplasma Source Type: research

Presence and prevalence of UV related genetic mutations in uveal melanoma: similarities with cutaneous melanoma.
This study hypothesized that a subset of UM tumors display evidence of genetic changes consistent with UV-related damage similar to that shown in CM. Analysis of the Broad Institute's Firebrowse depository of 80 UM samples and 343 CM samples, together with the Sanger Institute's Catalogue of Somatic Mutations in Cancer depository of 995 UM and 12,447 CM samples was undertaken to identify the most frequently mutated genes, mutation types and specific nucleotide variants (SNVs) in each condition. Somatic mutation data was cross-correlated and shared mutations assessed against known effects of UV radiation. The proportion of ...
Source: Neoplasma - April 16, 2020 Category: Cancer & Oncology Authors: Goh AY, Ramlogan-Steel CA, Jenkins KS, Steel JC, Layton CJ Tags: Neoplasma Source Type: research

MiR-33a-5p inhibits the growth and metastasis of melanoma cells by targeting SNAI2.
In conclusion, miR-33a-5p repressed the progression of melanoma by targeting SNAI2 via inactivation of the PI3K/AKT/mTOR signaling pathway, providing a potential molecular mechanism for the treatment of melanoma. PMID: 32305057 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - April 16, 2020 Category: Cancer & Oncology Authors: Zhang ZR, Yang N Tags: Neoplasma Source Type: research

O-GlcNAcylation promotes malignant phenotypes of bladder cancer cells.
Abstract O-GlcNAcylation (O-GlcNAc) is a posttranslational modification that is mediated by O-GlcNAc-transferase (OGT) and reversed by O-GlcNAcase (OGA). Increasing evidence indicates that protein O-GlcNAcylation is increased in various types of cancer. In the present study, we aimed to evaluate the expression and function of both OGT and OGA in bladder cancer cells in vitro and in vivo. Expression data of OGT and OGA at the mRNA level was obtained from the Oncomine database. Effects of OGT and OGA on cell proliferate, invasive and migratory abilities were assessed using MTT, wound healing, cell invasive assay, an...
Source: Neoplasma - April 16, 2020 Category: Cancer & Oncology Authors: Jin L, Lu MH, Dai GC, Yao Q, Xiang H, Wang LX, Xue BX, Liu X Tags: Neoplasma Source Type: research

miR-326 inhibits the progression of papillary thyroid carcinoma by targeting MAPK1 and ERBB4.
Abstract Papillary thyroid carcinoma (PTC) is the prevalent histotype of thyroid cancer, with increasing incidence worldwide. MicroRNAs (miRNAs) could play an important role in the development and progression of human cancers. Interestingly, miR-326 was validated as one of the downregulated miRNAs in PTC. Therefore, it is necessary to research the function of miR-326 involved in the progression of PTC. In the current study, we detected the downregulation of miR-326 in PTC tissues and cell lines. The miR-326 overexpression or knockdown was conducted in TPC-1 or HTh83 PTC cells. miR-326 mimics decreased the prolifer...
Source: Neoplasma - April 7, 2020 Category: Cancer & Oncology Authors: Nie FR, Li QX, Wei HF, Ma Y Tags: Neoplasma Source Type: research