Identification of plasma SAA2 as a candidate biomarker for the detection and surveillance of non-small cell lung cancer
This study aimed to measure the expression of SAA2 in plasma and to assess its diagnostic efficacy as a biomarker for non-small cell lung cancer (NSCLC). The gene expression of SAA2 in NSCLC was analyzed based on a database. Then, SAA2 expression was detected by immunohistochemistry in lung tissue and by enzyme-linked immunosorbent assay in 90 patients with NSCLC and 61 normal controls. Finally, the diagnostic performance was assessed in terms of accuracy, sensitivity, and specificity. At the gene and protein levels, the SAA2 expression was significantly higher in the NSCLC group than in the control group (p
Source: Neoplasma - October 14, 2021 Category: Cancer & Oncology Authors: Fa-Fu Zhang Bin Han Run-Hao Xu Qing-Qing Zhu Qi-Qi Wu Hui-Min Wei Zhi-Lei Cui Shu-Lin Zhang Min-Jie Meng Source Type: research

Inhibiting endoplasmic reticulum stress mediated-autophagy enhances the pro-apoptotic effects of resveratrol derivative in colon cancer cells
Neoplasma. 2021 Oct 14:210422N552. doi: 10.4149/neo_2021_210422N552. Online ahead of print.ABSTRACTPrevious studies have demonstrated that endoplasmic reticulum stress (ERS) might play a major role in inducing cellular autophagy and apoptosis in multiple types of cancer. Herein, we observed that trans-3, 5, 4'-trimethoxystilbene (TMS) exposure facilitated apoptotic cell death and ERS-mediated autophagy in colon cancer SW480 and HCT116 cells. Interestingly, our data demonstrated that ERS was not involved in TMS-induced apoptosis. However, ERS notably induced protective autophagy in SW480 and HCT116 cells. In addition, inhib...
Source: Neoplasma - October 14, 2021 Category: Cancer & Oncology Authors: Lei Yu Ji-Guang Hou Yun-Feng Li Yin Wang Ming Hong Yan-Ming Yang Source Type: research

Phycocyanin inhibits pancreatic cancer metastasis via suppressing epithelial-mesenchymal transition and targeting Akt/ β-catenin pathway
This study was aimed to investigate whether phycocyanin inhibits pancreatic cancer cells' metastasis and the potential underlying mechanisms. Human pancreatic carcinoma cell lines PANC-1 and BxPC3 were treated with phycocyanin (5, 10, 20, and 40 μM) for 48 h or 72 h. BALB/c nude mice were inoculated intravenously with luciferase-PANC-1 cells to establish a tumor metastasis model. BALB/c nude mice were also inoculated subcutaneously with PANC-1 cells to establish a xenograft tumor model. Our results indicated that (1) phycocyanin inhibited migration, invasion, and movement of pancreatic cancer cells in vitro, and tumor m...
Source: Neoplasma - October 14, 2021 Category: Cancer & Oncology Authors: Rongrong Huang Gaoyong Liao Meifeng Gao Yu Ou Source Type: research

circRNA_0006470 promotes the proliferation and migration of gastric cancer cells by functioning as a sponge of miR-27b-3p
In conclusion, hsa_circ_0006470 promoted GC cell proliferation and migration through targeting miR-27b-3p and suppressing autophagy machinery.PMID:34641696 | DOI:10.4149/neo_2021_210222N235 (Source: Neoplasma)
Source: Neoplasma - October 13, 2021 Category: Cancer & Oncology Authors: Yejia Cui Jin Cao Shaolong Huang Jinjun Ye Haohai Huang Dan Liao Yufeng Yang Aiping Yin Wanchan Chen Yelin Yao Yingai He Rong Pu Source Type: research

A novel intensive conditioning regimen for allogeneic hematopoietic stem cell transplantation in the treatment of relapsed/refractory acute myeloid leukemia
Neoplasma. 2021 Oct 11:210426N573. doi: 10.4149/neo_2021_210426N573. Online ahead of print.ABSTRACTWe conducted a prospective study to evaluate the efficacy and safety of cladribine, cytarabine, mitoxantrone, and granulocyte colony-stimulating factor (CLAG-M) regimen combined with busulfan and cyclophosphamide (Bucy) as new intensive conditioning before allogeneic hematopoietic stem cell transplantation (allo-HSCT) in the treatment of relapsed/refractory acute myeloid leukemia (AML). 24 patients were enrolled. The median follow-up was 15.2 months (range 1.9-67.0 months). Except for one patient who died before graft infusio...
Source: Neoplasma - October 13, 2021 Category: Cancer & Oncology Authors: Yanling Sun Jingwen Zhang Bing Long Xiangzhong Zhang Ruijuan Wen Yi He Xudong Li Source Type: research

LncRNA LINC00355 promotes EMT and metastasis of bladder cancer cells through the miR-424-5p/HMGA2 axis
In this study, we aim to investigate the role of lncRNA LINC00355 in bladder cancer progression and metastasis. The association between LINC00355 and the prognosis of bladder cancer patients was determined by Kaplan-Meier survival analysis. Cell migration and invasion ability were detected using the Transwell migration and invasion assay. The relationships of LINC00355, miR-424-5p, and High Mobility Group AT-Hook 2 (HMGA2) were verified through the luciferase assay and RNA pull-down assay. Xenograft tumor was established to evaluated tumor lung metastasis in vivo. qRT-PCR and western blot were used to detect gene expressio...
Source: Neoplasma - October 13, 2021 Category: Cancer & Oncology Authors: Wen-Jie Li Gang Li Zhong-Wen Liu Zhi-Yong Chen Rong Pu Source Type: research

Detection of therapeutically relevant and concomitant rare somatic variants in colorectal cancer
Neoplasma. 2021 Oct 11:210616N804. doi: 10.4149/neo_2021_210616N804. Online ahead of print.ABSTRACTIn colorectal cancer, clinically relevant biomarkers are known for genome-guided therapy that can be detected by both first and next generation methods. The aim of our work was to introduce a robust NGS assay that will be able to detect, in addition to standard predictive single nucleotide-based biomarkers, even rare and concomitant clinically relevant variants. Another aim was to identify truncating mutations in APC and pathogenic variants in TP53 to divide patients into potentially prognostic groups. A multigene panel with ...
Source: Neoplasma - October 13, 2021 Category: Cancer & Oncology Authors: Peter Mikolajcik Zora Lasabova Dusan Loderer Marian Grendar Michal Kalman Ivana Kasubova Vincent Lucansky Alexander Johannes Wiederhold Juraj Marcinek Tatiana Burjanivova Eva Kudelova Martin Vojtko Adam Svec Lukas Plank Jan Janik Ludovit Laca Source Type: research

circRNA_0006470 promotes the proliferation and migration of gastric cancer cells by functioning as a sponge of miR-27b-3p
In conclusion, hsa_circ_0006470 promoted GC cell proliferation and migration through targeting miR-27b-3p and suppressing autophagy machinery.PMID:34641696 | DOI:10.4149/neo_2021_210222N235 (Source: Neoplasma)
Source: Neoplasma - October 13, 2021 Category: Cancer & Oncology Authors: Yejia Cui Jin Cao Shaolong Huang Jinjun Ye Haohai Huang Dan Liao Yufeng Yang Aiping Yin Wanchan Chen Yelin Yao Yingai He Rong Pu Source Type: research

A novel intensive conditioning regimen for allogeneic hematopoietic stem cell transplantation in the treatment of relapsed/refractory acute myeloid leukemia
Neoplasma. 2021 Oct 11:210426N573. doi: 10.4149/neo_2021_210426N573. Online ahead of print.ABSTRACTWe conducted a prospective study to evaluate the efficacy and safety of cladribine, cytarabine, mitoxantrone, and granulocyte colony-stimulating factor (CLAG-M) regimen combined with busulfan and cyclophosphamide (Bucy) as new intensive conditioning before allogeneic hematopoietic stem cell transplantation (allo-HSCT) in the treatment of relapsed/refractory acute myeloid leukemia (AML). 24 patients were enrolled. The median follow-up was 15.2 months (range 1.9-67.0 months). Except for one patient who died before graft infusio...
Source: Neoplasma - October 13, 2021 Category: Cancer & Oncology Authors: Yanling Sun Jingwen Zhang Bing Long Xiangzhong Zhang Ruijuan Wen Yi He Xudong Li Source Type: research

LncRNA LINC00355 promotes EMT and metastasis of bladder cancer cells through the miR-424-5p/HMGA2 axis
In this study, we aim to investigate the role of lncRNA LINC00355 in bladder cancer progression and metastasis. The association between LINC00355 and the prognosis of bladder cancer patients was determined by Kaplan-Meier survival analysis. Cell migration and invasion ability were detected using the Transwell migration and invasion assay. The relationships of LINC00355, miR-424-5p, and High Mobility Group AT-Hook 2 (HMGA2) were verified through the luciferase assay and RNA pull-down assay. Xenograft tumor was established to evaluated tumor lung metastasis in vivo. qRT-PCR and western blot were used to detect gene expressio...
Source: Neoplasma - October 13, 2021 Category: Cancer & Oncology Authors: Wen-Jie Li Gang Li Zhong-Wen Liu Zhi-Yong Chen Rong Pu Source Type: research

Detection of therapeutically relevant and concomitant rare somatic variants in colorectal cancer
Neoplasma. 2021 Oct 11:210616N804. doi: 10.4149/neo_2021_210616N804. Online ahead of print.ABSTRACTIn colorectal cancer, clinically relevant biomarkers are known for genome-guided therapy that can be detected by both first and next generation methods. The aim of our work was to introduce a robust NGS assay that will be able to detect, in addition to standard predictive single nucleotide-based biomarkers, even rare and concomitant clinically relevant variants. Another aim was to identify truncating mutations in APC and pathogenic variants in TP53 to divide patients into potentially prognostic groups. A multigene panel with ...
Source: Neoplasma - October 13, 2021 Category: Cancer & Oncology Authors: Peter Mikolajcik Zora Lasabova Dusan Loderer Marian Grendar Michal Kalman Ivana Kasubova Vincent Lucansky Alexander Johannes Wiederhold Juraj Marcinek Tatiana Burjanivova Eva Kudelova Martin Vojtko Adam Svec Lukas Plank Jan Janik Ludovit Laca Source Type: research

Tumor-associated macrophages increase COX-2 expression promoting endocrine resistance in breast cancer via the PI3K/Akt/mTOR pathway
This article is dedicated to discuss the role of TAMs in the endocrine resistance of breast cancer. It was found that tamoxifen-resistant MCF-7 cells induced more macrophages polarized into TAMs. Conversely, TAMs increased the expression of cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2), which promoted tamoxifen resistance through the activation of the PI3K/Akt/mTOR signaling pathway in MCF-7 cells. Furthermore, clinical analysis supported that five-year progression-free survival (PFS) of breast cancer patients with abundant COX-2 expression in TAMs was shorter (p
Source: Neoplasma - October 8, 2021 Category: Cancer & Oncology Authors: Qi Qin Hongfei Ji Dongbo Li Han Zhang Ziwen Zhang Qingyuan Zhang Source Type: research

Tumor-associated macrophages increase COX-2 expression promoting endocrine resistance in breast cancer via the PI3K/Akt/mTOR pathway
This article is dedicated to discuss the role of TAMs in the endocrine resistance of breast cancer. It was found that tamoxifen-resistant MCF-7 cells induced more macrophages polarized into TAMs. Conversely, TAMs increased the expression of cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2), which promoted tamoxifen resistance through the activation of the PI3K/Akt/mTOR signaling pathway in MCF-7 cells. Furthermore, clinical analysis supported that five-year progression-free survival (PFS) of breast cancer patients with abundant COX-2 expression in TAMs was shorter (p
Source: Neoplasma - October 8, 2021 Category: Cancer & Oncology Authors: Qi Qin Hongfei Ji Dongbo Li Han Zhang Ziwen Zhang Qingyuan Zhang Source Type: research

Tumor-associated macrophages increase COX-2 expression promoting endocrine resistance in breast cancer via the PI3K/Akt/mTOR pathway
This article is dedicated to discuss the role of TAMs in the endocrine resistance of breast cancer. It was found that tamoxifen-resistant MCF-7 cells induced more macrophages polarized into TAMs. Conversely, TAMs increased the expression of cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2), which promoted tamoxifen resistance through the activation of the PI3K/Akt/mTOR signaling pathway in MCF-7 cells. Furthermore, clinical analysis supported that five-year progression-free survival (PFS) of breast cancer patients with abundant COX-2 expression in TAMs was shorter (p
Source: Neoplasma - October 8, 2021 Category: Cancer & Oncology Authors: Qi Qin Hongfei Ji Dongbo Li Han Zhang Ziwen Zhang Qingyuan Zhang Source Type: research

Tumor-associated macrophages increase COX-2 expression promoting endocrine resistance in breast cancer via the PI3K/Akt/mTOR pathway
This article is dedicated to discuss the role of TAMs in the endocrine resistance of breast cancer. It was found that tamoxifen-resistant MCF-7 cells induced more macrophages polarized into TAMs. Conversely, TAMs increased the expression of cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2), which promoted tamoxifen resistance through the activation of the PI3K/Akt/mTOR signaling pathway in MCF-7 cells. Furthermore, clinical analysis supported that five-year progression-free survival (PFS) of breast cancer patients with abundant COX-2 expression in TAMs was shorter (p
Source: Neoplasma - October 8, 2021 Category: Cancer & Oncology Authors: Qi Qin Hongfei Ji Dongbo Li Han Zhang Ziwen Zhang Qingyuan Zhang Source Type: research

Tumor-associated macrophages increase COX-2 expression promoting endocrine resistance in breast cancer via the PI3K/Akt/mTOR pathway
This article is dedicated to discuss the role of TAMs in the endocrine resistance of breast cancer. It was found that tamoxifen-resistant MCF-7 cells induced more macrophages polarized into TAMs. Conversely, TAMs increased the expression of cyclooxygenase-2 (COX-2)/prostaglandin E2 (PGE2), which promoted tamoxifen resistance through the activation of the PI3K/Akt/mTOR signaling pathway in MCF-7 cells. Furthermore, clinical analysis supported that five-year progression-free survival (PFS) of breast cancer patients with abundant COX-2 expression in TAMs was shorter (p
Source: Neoplasma - October 8, 2021 Category: Cancer & Oncology Authors: Qi Qin Hongfei Ji Dongbo Li Han Zhang Ziwen Zhang Qingyuan Zhang Source Type: research

Photodynamic therapy of multidrug resistant leukemic murine cells by 3,6-bis(alkylthiourea)acridine hydrochlorides
In this study, we investigated the efficacy of PDT/AcrDTUs for the treatment of L1210/VCR cells as a model of chemo-resistant cells (overexpressing P-glycoprotein, P-gp). The photoactivation (365 nm, 1.05 J/cm2) increased the cytotoxicity of AcrDTUs 10 - 15 times. Inhibition of P-gp (verapamil) has been shown to have no significant effect on the accumulation of propyl-AcrDTU (the most potent derivative) in L1210/VCR cells. The intracellular distribution of this acridine derivative has been studied. Prior to irradiation of the resistant cells, propyl-AcrDTU was sequestered mainly in the cytosol, partly in the mitochondria, ...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Helena Paul íková Al žbeta Cisáriková Zuzana Ba čová Ladislav Janovec J án Imrich M ário Šereš Ľuba Hunáková Source Type: research

Predictive model using hemoglobin, albumin, fibrinogen, and neutrophil-to-lymphocyte ratio to distinguish patients with colorectal cancer from those with benign adenoma
Neoplasma. 2021 Sep 29:210331N435. doi: 10.4149/neo_2021_210331N435. Online ahead of print.ABSTRACTColorectal cancer (CRC) is associated with inflammation, activation of coagulation, and mild anemia. Hematological parameters reflecting ongoing cancer may have the potency to be effective for early diagnostics of CRC. The aim of this study was to examine the validity and relationship between some biochemical and hematological parameters for the early diagnosis of CRC. We designed a prospective observational cohort study to analyze whether these laboratory markers have the potency to distinguish benign tumors from malignant b...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Roman Z áhorec V ítězslav Marek Iveta Waczulikova Tom áš Veselovský Julius Palaj Štefan Kečkéš Štefan Durdík Source Type: research

NCAPG, mediated by miR-378a-3p, regulates cell proliferation, cell cycle progression, and apoptosis of oral squamous cell carcinoma through the GSK-3 β/β-catenin signaling
This study was aimed to investigate the role of NCAPG on OSCC pathogenesis. NCAPG mRNA expression data in OSCC tissues were obtained from the Gene Expression Omnibus (GEO) database and NCAPG protein expression in OSCC cell lines was determined by western blotting analysis. The results demonstrated that NCAPG expression in OSCC tissues and cells was higher than that of normal control. Following the short interfering RNA (siRNA) knockdown of NCAPG in two OSCC cell lines, we observed that NCAPG depletion notably inhibited OSCC proliferation and cell cycle progression, as well as promoted apoptosis in vitro. Besides, silencing...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Jianing Li Shiqun Sun Jia Li Xuetao Zhao Zehua Li Tong Sha Zhi Cui Source Type: research

Mere15, a novel polypeptide from Meretrix meretrix, inhibits proliferation and metastasis of human non-small cell lung cancer cells through regulating the PI3K/Akt/mTOR signaling pathway
Neoplasma. 2021 Sep 29:210509N628. doi: 10.4149/neo_2021_210509N628. Online ahead of print.ABSTRACTMere15, an anticancer polypeptide with a molecular weight of 15 kDa, is extracted from the marine species Meretrix meretrix. A previous study in our laboratory has confirmed that Mere15 displays a potent antitumor activity. However, the underlying mechanism of Mere15 still remains unclear. The effect of Mere15 on the growth of a variety of tumor cells was measured by the CCK-8 assay. Hoechst33342/PI double staining and flow cytometry assays were used to detect the apoptosis status of cancer cells. Western blotting was used to...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Biantiao Xu Mingyue Guo Li Ma Ammad Ahmad Farooqi Linlin Wang Gan Qiao Minghua Liu Ling Zuo Hanlin Ye Xiukun Lin Shousong Cao Source Type: research

The matrix metalloproteinase gene family: a significant prognostic gene lineage correlated with immune infiltrates in laryngeal squamous cell carcinoma
This study aims to elucidate the potential genes of the matrix metalloproteinase (MMP) family, responsible for the progression of laryngeal squamous cell carcinoma (LSCC). Besides, we ascertained the changes in common malignant behaviors in vitro by knocking down MMP1. TCGA, GEO, Oncomine, and Microarray data were conducted to analyze the expression levels of MMPs and to find tissue-specific genes in LSCC. Univariate and multivariate Cox regression analyses were established in the construction of a prognostic model based on expression profiles and clinical information of LSCC in TCGA. We then comprehensively analyzed survi...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Chuan Yang Huan Cao Jian-Wang Yang Tao Liu Jian-Tao Wang Bao-Shan Wang Source Type: research

Photodynamic therapy of multidrug resistant leukemic murine cells by 3,6-bis(alkylthiourea)acridine hydrochlorides
In this study, we investigated the efficacy of PDT/AcrDTUs for the treatment of L1210/VCR cells as a model of chemo-resistant cells (overexpressing P-glycoprotein, P-gp). The photoactivation (365 nm, 1.05 J/cm2) increased the cytotoxicity of AcrDTUs 10 - 15 times. Inhibition of P-gp (verapamil) has been shown to have no significant effect on the accumulation of propyl-AcrDTU (the most potent derivative) in L1210/VCR cells. The intracellular distribution of this acridine derivative has been studied. Prior to irradiation of the resistant cells, propyl-AcrDTU was sequestered mainly in the cytosol, partly in the mitochondria, ...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Helena Paul íková Al žbeta Cisáriková Zuzana Ba čová Ladislav Janovec J án Imrich M ário Šereš Ľuba Hunáková Source Type: research

Predictive model using hemoglobin, albumin, fibrinogen, and neutrophil-to-lymphocyte ratio to distinguish patients with colorectal cancer from those with benign adenoma
Neoplasma. 2021 Sep 29:210331N435. doi: 10.4149/neo_2021_210331N435. Online ahead of print.ABSTRACTColorectal cancer (CRC) is associated with inflammation, activation of coagulation, and mild anemia. Hematological parameters reflecting ongoing cancer may have the potency to be effective for early diagnostics of CRC. The aim of this study was to examine the validity and relationship between some biochemical and hematological parameters for the early diagnosis of CRC. We designed a prospective observational cohort study to analyze whether these laboratory markers have the potency to distinguish benign tumors from malignant b...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Roman Z áhorec V ítězslav Marek Iveta Waczulikova Tom áš Veselovský Julius Palaj Štefan Kečkéš Štefan Durdík Source Type: research

NCAPG, mediated by miR-378a-3p, regulates cell proliferation, cell cycle progression, and apoptosis of oral squamous cell carcinoma through the GSK-3 β/β-catenin signaling
This study was aimed to investigate the role of NCAPG on OSCC pathogenesis. NCAPG mRNA expression data in OSCC tissues were obtained from the Gene Expression Omnibus (GEO) database and NCAPG protein expression in OSCC cell lines was determined by western blotting analysis. The results demonstrated that NCAPG expression in OSCC tissues and cells was higher than that of normal control. Following the short interfering RNA (siRNA) knockdown of NCAPG in two OSCC cell lines, we observed that NCAPG depletion notably inhibited OSCC proliferation and cell cycle progression, as well as promoted apoptosis in vitro. Besides, silencing...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Jianing Li Shiqun Sun Jia Li Xuetao Zhao Zehua Li Tong Sha Zhi Cui Source Type: research

Mere15, a novel polypeptide from Meretrix meretrix, inhibits proliferation and metastasis of human non-small cell lung cancer cells through regulating the PI3K/Akt/mTOR signaling pathway
Neoplasma. 2021 Sep 29:210509N628. doi: 10.4149/neo_2021_210509N628. Online ahead of print.ABSTRACTMere15, an anticancer polypeptide with a molecular weight of 15 kDa, is extracted from the marine species Meretrix meretrix. A previous study in our laboratory has confirmed that Mere15 displays a potent antitumor activity. However, the underlying mechanism of Mere15 still remains unclear. The effect of Mere15 on the growth of a variety of tumor cells was measured by the CCK-8 assay. Hoechst33342/PI double staining and flow cytometry assays were used to detect the apoptosis status of cancer cells. Western blotting was used to...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Biantiao Xu Mingyue Guo Li Ma Ammad Ahmad Farooqi Linlin Wang Gan Qiao Minghua Liu Ling Zuo Hanlin Ye Xiukun Lin Shousong Cao Source Type: research

The matrix metalloproteinase gene family: a significant prognostic gene lineage correlated with immune infiltrates in laryngeal squamous cell carcinoma
This study aims to elucidate the potential genes of the matrix metalloproteinase (MMP) family, responsible for the progression of laryngeal squamous cell carcinoma (LSCC). Besides, we ascertained the changes in common malignant behaviors in vitro by knocking down MMP1. TCGA, GEO, Oncomine, and Microarray data were conducted to analyze the expression levels of MMPs and to find tissue-specific genes in LSCC. Univariate and multivariate Cox regression analyses were established in the construction of a prognostic model based on expression profiles and clinical information of LSCC in TCGA. We then comprehensively analyzed survi...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Chuan Yang Huan Cao Jian-Wang Yang Tao Liu Jian-Tao Wang Bao-Shan Wang Source Type: research

Photodynamic therapy of multidrug resistant leukemic murine cells by 3,6-bis(alkylthiourea)acridine hydrochlorides
In this study, we investigated the efficacy of PDT/AcrDTUs for the treatment of L1210/VCR cells as a model of chemo-resistant cells (overexpressing P-glycoprotein, P-gp). The photoactivation (365 nm, 1.05 J/cm2) increased the cytotoxicity of AcrDTUs 10 - 15 times. Inhibition of P-gp (verapamil) has been shown to have no significant effect on the accumulation of propyl-AcrDTU (the most potent derivative) in L1210/VCR cells. The intracellular distribution of this acridine derivative has been studied. Prior to irradiation of the resistant cells, propyl-AcrDTU was sequestered mainly in the cytosol, partly in the mitochondria, ...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Helena Paul íková Al žbeta Cisáriková Zuzana Ba čová Ladislav Janovec J án Imrich M ário Šereš Ľuba Hunáková Source Type: research

Predictive model using hemoglobin, albumin, fibrinogen, and neutrophil-to-lymphocyte ratio to distinguish patients with colorectal cancer from those with benign adenoma
Neoplasma. 2021 Sep 29:210331N435. doi: 10.4149/neo_2021_210331N435. Online ahead of print.ABSTRACTColorectal cancer (CRC) is associated with inflammation, activation of coagulation, and mild anemia. Hematological parameters reflecting ongoing cancer may have the potency to be effective for early diagnostics of CRC. The aim of this study was to examine the validity and relationship between some biochemical and hematological parameters for the early diagnosis of CRC. We designed a prospective observational cohort study to analyze whether these laboratory markers have the potency to distinguish benign tumors from malignant b...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Roman Z áhorec V ítězslav Marek Iveta Waczulikova Tom áš Veselovský Julius Palaj Štefan Kečkéš Štefan Durdík Source Type: research

NCAPG, mediated by miR-378a-3p, regulates cell proliferation, cell cycle progression, and apoptosis of oral squamous cell carcinoma through the GSK-3 β/β-catenin signaling
This study was aimed to investigate the role of NCAPG on OSCC pathogenesis. NCAPG mRNA expression data in OSCC tissues were obtained from the Gene Expression Omnibus (GEO) database and NCAPG protein expression in OSCC cell lines was determined by western blotting analysis. The results demonstrated that NCAPG expression in OSCC tissues and cells was higher than that of normal control. Following the short interfering RNA (siRNA) knockdown of NCAPG in two OSCC cell lines, we observed that NCAPG depletion notably inhibited OSCC proliferation and cell cycle progression, as well as promoted apoptosis in vitro. Besides, silencing...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Jianing Li Shiqun Sun Jia Li Xuetao Zhao Zehua Li Tong Sha Zhi Cui Source Type: research

Mere15, a novel polypeptide from Meretrix meretrix, inhibits proliferation and metastasis of human non-small cell lung cancer cells through regulating the PI3K/Akt/mTOR signaling pathway
Neoplasma. 2021 Sep 29:210509N628. doi: 10.4149/neo_2021_210509N628. Online ahead of print.ABSTRACTMere15, an anticancer polypeptide with a molecular weight of 15 kDa, is extracted from the marine species Meretrix meretrix. A previous study in our laboratory has confirmed that Mere15 displays a potent antitumor activity. However, the underlying mechanism of Mere15 still remains unclear. The effect of Mere15 on the growth of a variety of tumor cells was measured by the CCK-8 assay. Hoechst33342/PI double staining and flow cytometry assays were used to detect the apoptosis status of cancer cells. Western blotting was used to...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Biantiao Xu Mingyue Guo Li Ma Ammad Ahmad Farooqi Linlin Wang Gan Qiao Minghua Liu Ling Zuo Hanlin Ye Xiukun Lin Shousong Cao Source Type: research

The matrix metalloproteinase gene family: a significant prognostic gene lineage correlated with immune infiltrates in laryngeal squamous cell carcinoma
This study aims to elucidate the potential genes of the matrix metalloproteinase (MMP) family, responsible for the progression of laryngeal squamous cell carcinoma (LSCC). Besides, we ascertained the changes in common malignant behaviors in vitro by knocking down MMP1. TCGA, GEO, Oncomine, and Microarray data were conducted to analyze the expression levels of MMPs and to find tissue-specific genes in LSCC. Univariate and multivariate Cox regression analyses were established in the construction of a prognostic model based on expression profiles and clinical information of LSCC in TCGA. We then comprehensively analyzed survi...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Chuan Yang Huan Cao Jian-Wang Yang Tao Liu Jian-Tao Wang Bao-Shan Wang Source Type: research

Photodynamic therapy of multidrug resistant leukemic murine cells by 3,6-bis(alkylthiourea)acridine hydrochlorides
In this study, we investigated the efficacy of PDT/AcrDTUs for the treatment of L1210/VCR cells as a model of chemo-resistant cells (overexpressing P-glycoprotein, P-gp). The photoactivation (365 nm, 1.05 J/cm2) increased the cytotoxicity of AcrDTUs 10 - 15 times. Inhibition of P-gp (verapamil) has been shown to have no significant effect on the accumulation of propyl-AcrDTU (the most potent derivative) in L1210/VCR cells. The intracellular distribution of this acridine derivative has been studied. Prior to irradiation of the resistant cells, propyl-AcrDTU was sequestered mainly in the cytosol, partly in the mitochondria, ...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Helena Paul íková Al žbeta Cisáriková Zuzana Ba čová Ladislav Janovec J án Imrich M ário Šereš Ľuba Hunáková Source Type: research

Predictive model using hemoglobin, albumin, fibrinogen, and neutrophil-to-lymphocyte ratio to distinguish patients with colorectal cancer from those with benign adenoma
Neoplasma. 2021 Sep 29:210331N435. doi: 10.4149/neo_2021_210331N435. Online ahead of print.ABSTRACTColorectal cancer (CRC) is associated with inflammation, activation of coagulation, and mild anemia. Hematological parameters reflecting ongoing cancer may have the potency to be effective for early diagnostics of CRC. The aim of this study was to examine the validity and relationship between some biochemical and hematological parameters for the early diagnosis of CRC. We designed a prospective observational cohort study to analyze whether these laboratory markers have the potency to distinguish benign tumors from malignant b...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Roman Z áhorec V ítězslav Marek Iveta Waczulikova Tom áš Veselovský Julius Palaj Štefan Kečkéš Štefan Durdík Source Type: research

NCAPG, mediated by miR-378a-3p, regulates cell proliferation, cell cycle progression, and apoptosis of oral squamous cell carcinoma through the GSK-3 β/β-catenin signaling
This study was aimed to investigate the role of NCAPG on OSCC pathogenesis. NCAPG mRNA expression data in OSCC tissues were obtained from the Gene Expression Omnibus (GEO) database and NCAPG protein expression in OSCC cell lines was determined by western blotting analysis. The results demonstrated that NCAPG expression in OSCC tissues and cells was higher than that of normal control. Following the short interfering RNA (siRNA) knockdown of NCAPG in two OSCC cell lines, we observed that NCAPG depletion notably inhibited OSCC proliferation and cell cycle progression, as well as promoted apoptosis in vitro. Besides, silencing...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Jianing Li Shiqun Sun Jia Li Xuetao Zhao Zehua Li Tong Sha Zhi Cui Source Type: research

Mere15, a novel polypeptide from Meretrix meretrix, inhibits proliferation and metastasis of human non-small cell lung cancer cells through regulating the PI3K/Akt/mTOR signaling pathway
Neoplasma. 2021 Sep 29:210509N628. doi: 10.4149/neo_2021_210509N628. Online ahead of print.ABSTRACTMere15, an anticancer polypeptide with a molecular weight of 15 kDa, is extracted from the marine species Meretrix meretrix. A previous study in our laboratory has confirmed that Mere15 displays a potent antitumor activity. However, the underlying mechanism of Mere15 still remains unclear. The effect of Mere15 on the growth of a variety of tumor cells was measured by the CCK-8 assay. Hoechst33342/PI double staining and flow cytometry assays were used to detect the apoptosis status of cancer cells. Western blotting was used to...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Biantiao Xu Mingyue Guo Li Ma Ammad Ahmad Farooqi Linlin Wang Gan Qiao Minghua Liu Ling Zuo Hanlin Ye Xiukun Lin Shousong Cao Source Type: research

The matrix metalloproteinase gene family: a significant prognostic gene lineage correlated with immune infiltrates in laryngeal squamous cell carcinoma
This study aims to elucidate the potential genes of the matrix metalloproteinase (MMP) family, responsible for the progression of laryngeal squamous cell carcinoma (LSCC). Besides, we ascertained the changes in common malignant behaviors in vitro by knocking down MMP1. TCGA, GEO, Oncomine, and Microarray data were conducted to analyze the expression levels of MMPs and to find tissue-specific genes in LSCC. Univariate and multivariate Cox regression analyses were established in the construction of a prognostic model based on expression profiles and clinical information of LSCC in TCGA. We then comprehensively analyzed survi...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Chuan Yang Huan Cao Jian-Wang Yang Tao Liu Jian-Tao Wang Bao-Shan Wang Source Type: research

Photodynamic therapy of multidrug resistant leukemic murine cells by 3,6-bis(alkylthiourea)acridine hydrochlorides
In this study, we investigated the efficacy of PDT/AcrDTUs for the treatment of L1210/VCR cells as a model of chemo-resistant cells (overexpressing P-glycoprotein, P-gp). The photoactivation (365 nm, 1.05 J/cm2) increased the cytotoxicity of AcrDTUs 10 - 15 times. Inhibition of P-gp (verapamil) has been shown to have no significant effect on the accumulation of propyl-AcrDTU (the most potent derivative) in L1210/VCR cells. The intracellular distribution of this acridine derivative has been studied. Prior to irradiation of the resistant cells, propyl-AcrDTU was sequestered mainly in the cytosol, partly in the mitochondria, ...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Helena Paul íková Al žbeta Cisáriková Zuzana Ba čová Ladislav Janovec J án Imrich M ário Šereš Ľuba Hunáková Source Type: research

Predictive model using hemoglobin, albumin, fibrinogen, and neutrophil-to-lymphocyte ratio to distinguish patients with colorectal cancer from those with benign adenoma
Neoplasma. 2021 Sep 29:210331N435. doi: 10.4149/neo_2021_210331N435. Online ahead of print.ABSTRACTColorectal cancer (CRC) is associated with inflammation, activation of coagulation, and mild anemia. Hematological parameters reflecting ongoing cancer may have the potency to be effective for early diagnostics of CRC. The aim of this study was to examine the validity and relationship between some biochemical and hematological parameters for the early diagnosis of CRC. We designed a prospective observational cohort study to analyze whether these laboratory markers have the potency to distinguish benign tumors from malignant b...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Roman Z áhorec V ítězslav Marek Iveta Waczulikova Tom áš Veselovský Julius Palaj Štefan Kečkéš Štefan Durdík Source Type: research

NCAPG, mediated by miR-378a-3p, regulates cell proliferation, cell cycle progression, and apoptosis of oral squamous cell carcinoma through the GSK-3 β/β-catenin signaling
This study was aimed to investigate the role of NCAPG on OSCC pathogenesis. NCAPG mRNA expression data in OSCC tissues were obtained from the Gene Expression Omnibus (GEO) database and NCAPG protein expression in OSCC cell lines was determined by western blotting analysis. The results demonstrated that NCAPG expression in OSCC tissues and cells was higher than that of normal control. Following the short interfering RNA (siRNA) knockdown of NCAPG in two OSCC cell lines, we observed that NCAPG depletion notably inhibited OSCC proliferation and cell cycle progression, as well as promoted apoptosis in vitro. Besides, silencing...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Jianing Li Shiqun Sun Jia Li Xuetao Zhao Zehua Li Tong Sha Zhi Cui Source Type: research

Mere15, a novel polypeptide from Meretrix meretrix, inhibits proliferation and metastasis of human non-small cell lung cancer cells through regulating the PI3K/Akt/mTOR signaling pathway
Neoplasma. 2021 Sep 29:210509N628. doi: 10.4149/neo_2021_210509N628. Online ahead of print.ABSTRACTMere15, an anticancer polypeptide with a molecular weight of 15 kDa, is extracted from the marine species Meretrix meretrix. A previous study in our laboratory has confirmed that Mere15 displays a potent antitumor activity. However, the underlying mechanism of Mere15 still remains unclear. The effect of Mere15 on the growth of a variety of tumor cells was measured by the CCK-8 assay. Hoechst33342/PI double staining and flow cytometry assays were used to detect the apoptosis status of cancer cells. Western blotting was used to...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Biantiao Xu Mingyue Guo Li Ma Ammad Ahmad Farooqi Linlin Wang Gan Qiao Minghua Liu Ling Zuo Hanlin Ye Xiukun Lin Shousong Cao Source Type: research

The matrix metalloproteinase gene family: a significant prognostic gene lineage correlated with immune infiltrates in laryngeal squamous cell carcinoma
This study aims to elucidate the potential genes of the matrix metalloproteinase (MMP) family, responsible for the progression of laryngeal squamous cell carcinoma (LSCC). Besides, we ascertained the changes in common malignant behaviors in vitro by knocking down MMP1. TCGA, GEO, Oncomine, and Microarray data were conducted to analyze the expression levels of MMPs and to find tissue-specific genes in LSCC. Univariate and multivariate Cox regression analyses were established in the construction of a prognostic model based on expression profiles and clinical information of LSCC in TCGA. We then comprehensively analyzed survi...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Chuan Yang Huan Cao Jian-Wang Yang Tao Liu Jian-Tao Wang Bao-Shan Wang Source Type: research

Photodynamic therapy of multidrug resistant leukemic murine cells by 3,6-bis(alkylthiourea)acridine hydrochlorides
In this study, we investigated the efficacy of PDT/AcrDTUs for the treatment of L1210/VCR cells as a model of chemo-resistant cells (overexpressing P-glycoprotein, P-gp). The photoactivation (365 nm, 1.05 J/cm2) increased the cytotoxicity of AcrDTUs 10 - 15 times. Inhibition of P-gp (verapamil) has been shown to have no significant effect on the accumulation of propyl-AcrDTU (the most potent derivative) in L1210/VCR cells. The intracellular distribution of this acridine derivative has been studied. Prior to irradiation of the resistant cells, propyl-AcrDTU was sequestered mainly in the cytosol, partly in the mitochondria, ...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Helena Paul íková Al žbeta Cisáriková Zuzana Ba čová Ladislav Janovec J án Imrich M ário Šereš Ľuba Hunáková Source Type: research

Predictive model using hemoglobin, albumin, fibrinogen, and neutrophil-to-lymphocyte ratio to distinguish patients with colorectal cancer from those with benign adenoma
Neoplasma. 2021 Sep 29:210331N435. doi: 10.4149/neo_2021_210331N435. Online ahead of print.ABSTRACTColorectal cancer (CRC) is associated with inflammation, activation of coagulation, and mild anemia. Hematological parameters reflecting ongoing cancer may have the potency to be effective for early diagnostics of CRC. The aim of this study was to examine the validity and relationship between some biochemical and hematological parameters for the early diagnosis of CRC. We designed a prospective observational cohort study to analyze whether these laboratory markers have the potency to distinguish benign tumors from malignant b...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Roman Z áhorec V ítězslav Marek Iveta Waczulikova Tom áš Veselovský Julius Palaj Štefan Kečkéš Štefan Durdík Source Type: research

NCAPG, mediated by miR-378a-3p, regulates cell proliferation, cell cycle progression, and apoptosis of oral squamous cell carcinoma through the GSK-3 β/β-catenin signaling
This study was aimed to investigate the role of NCAPG on OSCC pathogenesis. NCAPG mRNA expression data in OSCC tissues were obtained from the Gene Expression Omnibus (GEO) database and NCAPG protein expression in OSCC cell lines was determined by western blotting analysis. The results demonstrated that NCAPG expression in OSCC tissues and cells was higher than that of normal control. Following the short interfering RNA (siRNA) knockdown of NCAPG in two OSCC cell lines, we observed that NCAPG depletion notably inhibited OSCC proliferation and cell cycle progression, as well as promoted apoptosis in vitro. Besides, silencing...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Jianing Li Shiqun Sun Jia Li Xuetao Zhao Zehua Li Tong Sha Zhi Cui Source Type: research

Mere15, a novel polypeptide from Meretrix meretrix, inhibits proliferation and metastasis of human non-small cell lung cancer cells through regulating the PI3K/Akt/mTOR signaling pathway
Neoplasma. 2021 Sep 29:210509N628. doi: 10.4149/neo_2021_210509N628. Online ahead of print.ABSTRACTMere15, an anticancer polypeptide with a molecular weight of 15 kDa, is extracted from the marine species Meretrix meretrix. A previous study in our laboratory has confirmed that Mere15 displays a potent antitumor activity. However, the underlying mechanism of Mere15 still remains unclear. The effect of Mere15 on the growth of a variety of tumor cells was measured by the CCK-8 assay. Hoechst33342/PI double staining and flow cytometry assays were used to detect the apoptosis status of cancer cells. Western blotting was used to...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Biantiao Xu Mingyue Guo Li Ma Ammad Ahmad Farooqi Linlin Wang Gan Qiao Minghua Liu Ling Zuo Hanlin Ye Xiukun Lin Shousong Cao Source Type: research

The matrix metalloproteinase gene family: a significant prognostic gene lineage correlated with immune infiltrates in laryngeal squamous cell carcinoma
This study aims to elucidate the potential genes of the matrix metalloproteinase (MMP) family, responsible for the progression of laryngeal squamous cell carcinoma (LSCC). Besides, we ascertained the changes in common malignant behaviors in vitro by knocking down MMP1. TCGA, GEO, Oncomine, and Microarray data were conducted to analyze the expression levels of MMPs and to find tissue-specific genes in LSCC. Univariate and multivariate Cox regression analyses were established in the construction of a prognostic model based on expression profiles and clinical information of LSCC in TCGA. We then comprehensively analyzed survi...
Source: Neoplasma - September 29, 2021 Category: Cancer & Oncology Authors: Chuan Yang Huan Cao Jian-Wang Yang Tao Liu Jian-Tao Wang Bao-Shan Wang Source Type: research

The role of corticotropin-releasing hormone (CRH) family in tumors
Neoplasma. 2021 Sep 17:210219N226. doi: 10.4149/neo_2021_210219N226. Online ahead of print.ABSTRACTThe corticotropin-releasing hormone (CRH) family is widely distributed among the central nervous system and peripheral tissues, such as the digestive, cardiovascular, immune, reproductive, endocrine systems. The CRH family members are widely involved in the regulation of human cell biological processes, immune response, and regulation of inflammatory processes that can affect the occurrence and development of tumors. At present, CRH family members and their receptors can be detected in many tumor tissues, and some people thin...
Source: Neoplasma - September 17, 2021 Category: Cancer & Oncology Authors: Su-Qin Yi Jia-Xing An Cheng-Cheng Liao Sha Lei Hai Jin Bi-Guang Tuo Source Type: research

microRNA-10a-5p overexpression suppresses malignancy of colon cancer by regulating human liver cancer fibroblasts
Neoplasma. 2021 Sep 17:210226N250. doi: 10.4149/neo_2021_210226N250. Online ahead of print.ABSTRACTThe crosstalk between tumor and stroma plays a critical role in cancer metastasis. However, the function of miR-10a-5p on liver fibroblasts in the metastatic microenvironment of colon cancer (CC) and the effect of activated fibroblasts on CC cells are still unclear. In our study, miR-10a-5p overexpression inhibited the proliferation, migration, and IL-6/IL-8 level of LX-2 and human liver cancer fibroblasts (HLCFs) cells. Moreover, miR-10a-5p had lower expression in HLCFs than in human liver normal fibroblasts (HLNFs). The con...
Source: Neoplasma - September 17, 2021 Category: Cancer & Oncology Authors: Xuan Zheng Jing-Wu Li Yan-Kun Liu Yi-Fu Ma Jian-Hui Gan Su-Gui Han Jian Wang Zhao-Yuan Wan Jun Zhang Yan Liu Yu-Feng Li Guang-Ling Zhang Source Type: research

Overexpression of BMP9 promotes ovarian cancer progression via Notch1 signaling
Neoplasma. 2021 Sep 17:210326N404. doi: 10.4149/neo_2021_210326N404. Online ahead of print.ABSTRACTCell proliferation and migration play important parts in ovarian cancer progression. BMP9, as one of the members of the TGF-β superfamily and BMP family, plays a diverse and significant array of biological roles, including cell differentiation, proliferation, apoptosis, tumorigenesis, and metabolism. However, the role and mechanism of BMP9 in ovarian cancer progression remains uncertain. We found that the expression of BMP9 was increased in human ovarian cancer cell lines, which induced Notch1 intracellular domain (NICD1...
Source: Neoplasma - September 17, 2021 Category: Cancer & Oncology Authors: Lijuan Yang Yingying Bai Chaihong Zhang Junhong Du Yuemei Cheng Qinganzi Wang Bo Zhang Yongxiu Yang Source Type: research

LAMC2 acts as a novel therapeutic target of cetuximab in laryngeal cancer
In conclusion, LAMC2 may act as a novel anti-cancer target in LC.PMID:34533031 | DOI:10.4149/neo_2021_210421N549 (Source: Neoplasma)
Source: Neoplasma - September 17, 2021 Category: Cancer & Oncology Authors: Qin-Ying Wang Yong-Cai Liu Shui-Hong Zhou Hai-Hong Chen Source Type: research