Upregulated circZMIZ1 promotes the proliferation of prostate cancer cells and is a valuable marker in plasma.
In conclusion, these results revealed that circZMIZ1 might serve as a novel biomarker and a treatment target for prostate cancer treatment. PMID: 31686520 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - November 4, 2019 Category: Cancer & Oncology Authors: Jiang H, Lv DJ, Song XL, Wang C, Yu YZ, Zhao SC Tags: Neoplasma Source Type: research

HMGA1-mediated miR-671-5p targets APC to promote metastasis of clear cell renal cell carcinoma through Wnt signaling.
This study aimed to investigate the effect of miR-671-5p on metastasis of clear cell renal cell carcinoma (ccRCC) and underlying mechanism involved. The migration and invasion of ccRCC cells were determined by transwell and boyden assays in vitro and in vivo. Genes mRNA and protein expression were detected by quantitative polymerase chain reaction (qPCR) and western blot analysis, respectively. The target gene of miRNA was confirmed by luciferase reporter assays. Transcriptional regulation of miRNA by transcription factor was detected by chromatin immunoprecipitation assay (ChIP). The expression of miRNA in clinical specim...
Source: Neoplasma - November 4, 2019 Category: Cancer & Oncology Authors: Chi XG, Meng XX, Ding DL, Xuan XH, Chen YZ, Cai Q, Wang A Tags: Neoplasma Source Type: research

Preliminary evaluation of GLP-1R PET in the diagnosis and risk stratification of pheochromocytomas.
In this study, we firstly synthesized a novel radiotracer 68Ga-NOTA-MAL-Cys39-exendin-4 that specifically targets GLP-1R and evaluated the performance of GLP-1R PET for the diagnosis and risk stratification of PHEOs. Cys39-exendin-4 was conjugated to NOTA-MAL and then radiolabeled with 68Ga. The reaction was completed within 20 min with a yield of 91.6 ± 2.8%. In vitro cell uptake studies validated its high specificity. PET images showed promising tumor visualization with high uptake (1.88 ± 0.10 %ID/g for PC-12 poorly differentiated tumors and 1.09 ± 0.003 %ID/g for PC-12 highly differentiated tumors ...
Source: Neoplasma - November 4, 2019 Category: Cancer & Oncology Authors: Li M, Liu Y, Xu Y, Li Y, Pan D, Wang L, Yan J, Wang X, Yang R, Yang M Tags: Neoplasma Source Type: research

miR-186-5p targeting SIX1 inhibits cisplatin resistance in non-small-cell lung cancer cells (NSCLCs).
In conclusion, upregulation of miR-186-5p suppresses cisplatin resistance in DDP resistant NSCLC cells both in vitro and in vivo presumably by targeting SIX1. PMID: 31686523 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - November 4, 2019 Category: Cancer & Oncology Authors: Liu X, Zhou X, Chen Y, Huang Y, He J, Luo H Tags: Neoplasma Source Type: research

Evidence of β-blockers drug repurposing for the treatment of triple negative breast cancer: A systematic review.
Evidence of β-blockers drug repurposing for the treatment of triple negative breast cancer: A systematic review. Neoplasma. 2019 Oct 09;: Authors: Spini A, Roberto G, Gini R, Bartolini C, Bazzani L, Donnini S, Crispino S, Ziche M Abstract Triple negative breast cancer (TNBC) is a particularly aggressive subtype of breast cancer (BC) for which limited therapeutic options are available. Recently, β-blockers (BBs) have been suggested to have favorable effects on the treatment of BC. The aim of this systematic review was to collect evidence from preclinical and clinical studies concerning the sc...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Spini A, Roberto G, Gini R, Bartolini C, Bazzani L, Donnini S, Crispino S, Ziche M Tags: Neoplasma Source Type: research

Effects of ME3 on the proliferation, invasion and metastasis of pancreatic cancer cells through epithelial-mesenchymal transition.
In conclusion, ME3 is extensively involved in carcinogenesis of pancreatic cancer and may become a new candidate target for diagnosis, treatment and prognosis of pancreatic cancer. PMID: 31607129 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Zhang Q, Li J, Tan XP, Zhao Q Tags: Neoplasma Source Type: research

Survival rate of colon and rectum cancer in Iran: A systematic review and meta-analysis.
Abstract Colorectal cancer is one of the deadliest cancers worldwide. Effective screening, surveillance, and prevention can decrease its incidence, mortality and burden. This meta-analysis aims to provide a pooled estimation of 5-year survival rate for colorectal cancer based on topography codes and treatment in Iranian population. A systematic search for literature was done in international and national databases up to July 2018. Twenty-seven studies from 4929 articles met the eligible criteria. The overall pooled 5-year survival rate of colorectal cancer, colon, rectal and sigmoid were estimated 56% (95% CI: 49,...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Panahi MH, Panahi H, Mahdavi Hezaveh A, Mansournia MA, Bidhendi Yarandi R Tags: Neoplasma Source Type: research

TTK contributes to tumor growth and metastasis of clear cell renal cell carcinoma by inducing cell proliferation and invasion.
Abstract The aim of study was to determine the expression levels of TTK in clear cell renal cell carcinoma (ccRCC) tissues and its possible link with the clinical pathologic characteristics and the prognosis of patients suffering this disease, and to further explore the potential role of TTK in the progression of ccRCC. Immunohistochemical (IHC) assays were performed to detect the expression levels of TTK in 112 samples of ccRCC tissues and corresponding non-tumor tissues. According to the results of IHC assays, patients were divided into TTK high expression and low expression group. Clinical analysis related to t...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Liu XD, Yao DW, Xin F Tags: Neoplasma Source Type: research

A MYC target long non-coding RNA GATA2-AS1 regulates non-small cell lung cancer growth.
Abstract Non-small cell lung cancer (NSCLC) is the most prevalent subtype of lung cancer histologically, and an increasing number of evidences have shown that long non-coding RNAs (lncRNAs) are involved in tumorigenesis during the past years. Here we found a long non-coding RNA, GATA2-AS1, repressed NSCLC cells proliferation via regulating GATA2. GATA2-AS1 gene is located at antisense strand of GATA2 on chromosome while GATA2-AS1 RNA interacts with GATA1 protein at promoter region of GATA2 and then inhibits its transcription. Moreover, GATA2-AS1 is transcriptionally repressed by MYC in NSCLC cells. To conclude, ou...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Zhang L, Gao L, Shao M, Sun GY Tags: Neoplasma Source Type: research

Chromosome 17 centromere amplification and chromosomal instability (CIN) in breast cancer: Pathogenic and therapeutic implications.
Abstract Chromosomal instability (CIN) is present in variable degrees in a significant percentage (up to 90%) of cancers and often portends adverse outcomes. However, it has not been incorporated in clinical practice as a prognostic marker due to lack of standardization and proof of clinical utility of assays to measure it, as well as uncertainties regarding optimal cut-offs. Amplification of the centromeric region of chromosome 17 as measured by In Situ Hybridization (ISH) of the CEP17 probe is used clinically as part of the ISH assay for HER2 status determination in breast cancer in cases with intermediate (2+) ...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Voutsadakis IA Tags: Neoplasma Source Type: research

Protein arginine methyltransferase 1 promotes epithelial-mesenchymal transition via TGF- β1/Smad pathway in hepatic carcinoma cells.
Protein arginine methyltransferase 1 promotes epithelial-mesenchymal transition via TGF-β1/Smad pathway in hepatic carcinoma cells. Neoplasma. 2019 Oct 09;: Authors: Wei H, Liu Y, Min J, Zhang Y, Wang J, Zhou M, Xiong E, Yu G, Zhou H, He J, Zeng J, Gong A, Xu M Abstract Protein arginine methyltransferase 1 (PRMT1) is dysregulated in a number of human cancers. Herein, we report that PRMT1 expression is directly associated with epithelial-mesenchymal transition (EMT) in hepatic carcinoma cells. Firstly, we find that PRMT1 expression is higher in hepatic carcinoma tissues than that in normal liver t...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Wei H, Liu Y, Min J, Zhang Y, Wang J, Zhou M, Xiong E, Yu G, Zhou H, He J, Zeng J, Gong A, Xu M Tags: Neoplasma Source Type: research

PCNA and GSK3 β interact with each other to regulate H1299 lung adenocarcinoma cells apoptosis.
PCNA and GSK3β interact with each other to regulate H1299 lung adenocarcinoma cells apoptosis. Neoplasma. 2019 Oct 09;: Authors: Liu XH, Tang DE, Dai Y, Gao XJ, Liu LX Abstract Glycogen synthase kinase beta (GSK3β) is considered as a promising target for lung cancer treatment and its inhibitor lithium chloride (LiCl) is widely regarded as having potent anti-proliferative and apoptosis-modulating activities. Proliferating cell nuclear antigen (PCNA), as an auxiliary protein for DNA polymerase delta which regulates DNA replication and repair, has been reported to play an important role in regu...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Liu XH, Tang DE, Dai Y, Gao XJ, Liu LX Tags: Neoplasma Source Type: research

The significance of calprotectin, CD147, APOA4 and DJ-1 in non-invasive detection of urinary bladder carcinoma.
Abstract Aim of the study is to define the diagnostic accuracy of selected urinary protein biomarkers in the non-invasive detection of primary and recurrent urothelial carcinoma of the urinary bladder. The urinary levels of calprotectin, CD147, APOA4 and protein deglycase DJ-1 were examined in 255 individuals, out of which there were 60 controls with non-malignant urological disease, 61 patients with a history of urinary bladder cancer with negative cytology and negative cystoscopy and 134 patients with urinary bladder cancer. Urinary concentrations of biomarkers were determined by Enzyme-Linked Immunosorbent Assa...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Soukup V, Capoun O, Pesl M, Vavrova L, Sobotka R, Levova K, Hanus T, Zima T, Kalousova M Tags: Neoplasma Source Type: research

STIL is upregulated in nasopharyngeal carcinoma tissues and promotes nasopharyngeal carcinoma proliferation, migration and invasion.
Abstract Nasopharyngeal carcinoma (NPC) is the most common primary malignancy that originates from the nasopharynx. Some regulatory networks involved in nasopharyngeal carcinoma have been reported, but the relevant genes have not been fully identified. We have used mRNA microarray to identify differential expression genes between NPC tissues and adjacent normal tissues. Then high-content shRNA screening was carried out to screen the genes that may control proliferation in nasopharyngeal carcinoma. Cell proliferation was monitored by MTT assays and Celigo image cytometry in vitro and subcutaneous transplantation mo...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Ouyang Y, Jin YB, Chen XP, Zhang GY, Mao SL, Ling F, Luo W Tags: Neoplasma Source Type: research

Long non-coding RNA HOTAIR promotes cell viability, migration and invasion in thyroid cancer cells by sponging miR-17-5p.
In this study, we mainly investigated the role of HOTAIR in thyroid cancer and potential mechanism. RT-qPCR was conducted to evaluate the expression of Homeobox Transcript Antisense Intergenic RNA (HOTAIR) and miR-17-5p in thyroid cancer tissues and 4 cell lines (TPC-1, FTC-133, B-CPAP and SW579). MTT assay was employed to analyze the cell viability of TPC-1 and FTC-133 cells. The migration and invasion of TPC-1 and FTC-133 cells were detected via Transwell assay. Luciferase reporter assay was used to validate the target of HOTAIR. In vivo tumor formation assay in nude mice was applied to investigate the impact of HOTAIR o...
Source: Neoplasma - October 9, 2019 Category: Cancer & Oncology Authors: Liu X, Liu G, Lu Y, Shi Y Tags: Neoplasma Source Type: research

Comparison of dysregulated long noncoding RNAs in lung adenocarcinoma and spinal metastasis: A genome-wide analysis.
Abstract Long noncoding RNAs (lncRNAs) have been shown to play crucial roles in cancer metastasis, yet the lncRNAs landscape of lung adenocarcinoma has not been completely characterized. The aim of this study was to assess the expression profile and potential function of lncRNA in lung adenocarcinoma and in spinal metastasis (SM). A genome-wide microarray analysis was conducted of lung adenocarcinoma and SM tissue from ten Chinese patients. A total of 3345 differentially expressed lncRNAs were detected. Of those, 761 lncRNAs were upregulated and 2584 were downregulated (fold-change> 2.0, P
Source: Neoplasma - August 4, 2019 Category: Cancer & Oncology Authors: Wang H, Wang S, Liu P, Ma Y, Hu A, Sun C, Dong J Tags: Neoplasma Source Type: research

LncRNA KCNQ1OT1 controls cell proliferation, differentiation and apoptosis by sponging miR-326 to regulate c-Myc expression in acute myeloid leukemia.
Abstract Long noncoding RNAs (lncRNAs) have been reported to play essential roles in development and treatment of acute myeloid leukemia (AML). However, the role of lncRNA potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1 (KCNQ1OT1) in AML progression and its mechanism remain largely unknown. The expressions of KCNQ1OT1, microRNA-326 (miR-326) and c-Myc were measured by quantitative real-time polymerase chain reaction and western blot, respectively. Phorbol myristate acetate (PMA) was used for cell differentiation. Cell proliferation, apoptosis and differentiation were measured by MTT a...
Source: Neoplasma - August 4, 2019 Category: Cancer & Oncology Authors: Cheng P, Lu P, Guan J, Zhou Y, Zou L, Yi X, Cheng H Tags: Neoplasma Source Type: research

Prognostic impact of prognostic nutritional index in advanced (stage IIIB/IV) non-small cell lung cancer patients.
In this study, we collected the clinical data of 315 patients with advanced (stage IIIB/IV) NSCLC who had received chemotherapy or epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) between January 2010 and June 2011. Survival curves were plotted using the Kaplan-Meier method. Multivariate analyses were used to evaluate prognostic significance of PNI in patients with advanced (stage IIIB/IV) NSCLC. In our analysis, we found that PNI (P = 0.001) was significantly associated with OS in patients with advanced (stage IIIB/IV) NSCLC, so is smoking (P
Source: Neoplasma - August 4, 2019 Category: Cancer & Oncology Authors: Li XL, Yao ZH, Wan YY, Mou XY, Ni YH, Sun EL, Zhang D, Lin DJ Tags: Neoplasma Source Type: research

Clinical impact of genomic analysis in children with B-acute lymphoblastic leukemia: A pilot study in Slovakia.
In this study we reviewed 133 B-ALL patients. We found that nearly 40% of them (52 cases) belonged to the B-other ALL group. Eighteen B-other ALL patients were subjected to the analysis using SNP-array. Overall, we identified 126 cytogenomic changes and in 4 patients the SNP array revealed clinically relevant markers of adverse prognosis and high relapse risk. Integrating identified genetic changes into clinical practice can bring improvement of prognosis assessment for children with ALL in Slovakia. PMID: 31390871 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - August 4, 2019 Category: Cancer & Oncology Authors: Vaska A, Makohusova M, Plevova K, Skalicka K, Cermak M, Chovanec F, Fabri O, Svec P, Kolenova A Tags: Neoplasma Source Type: research

NKAP plays an oncogenic function partly through AKT signaling pathway in hepatocellular carcinoma.
Abstract NF-kB activating protein (NKAP) is a highly conserved protein involved in transcriptional repression, immune cell development, maturation, T cell acquisition of functional competency and maintenance of hematopoiesis. Here we first explore the function of NKAP in hepatocellular carcinoma (HCC). We found that NKAP was highly expressed in HCC tissues and associated with a poor patient survival. CCK8 assay showed that NKAP knockdown significantly decreased cell viability of HuH7 and Hep3B HCC cell lines. Cell invasion, tested by transwell assays, was significantly inhibited by NKAP knockdown in HuH7 and Hep3B cells (P
Source: Neoplasma - July 12, 2019 Category: Cancer & Oncology Authors: Song T, Liu JY, Yang JJ Tags: Neoplasma Source Type: research

Sevoflurane inhibits migration and invasion of colorectal cancer cells by regulating microRNA-34a/ADAM10 axis.
In this study, CRC HCT116 and SW480 cells were treated by various concentrations of sevoflurane. MTT assay and Transwell assay were applied to evaluate the cell viability, migration and invasion abilities of CRC cell lines, respectively. Real-time quantitative PCR (RT-qPCR) was used to examine the expression level of miR-34a, and western blot assay was employed to detect the protein level of ADAM10. The target interaction between miR-34a and ADAM10 was verified through bioinformatics analysis, luciferase reporter gene assay system. We found Aberrant inhibitory effects induced by sevoflurane on the cell viability, migration...
Source: Neoplasma - July 12, 2019 Category: Cancer & Oncology Authors: Sun SQ, Ren LJ, Liu J, Wang P, Shan SM Tags: Neoplasma Source Type: research

Prognostic significance of preoperative radiotherapy in stage II and III rectal cancer patients: A Strobe-compliant study of SEER 18 registries database (1988-2011).
This study was aimed to measure the efficacy of preoperative radiation therapy for survival in stage II and III rectal cancer patients. Retrospective cohort study used the database of Surveillance, Epidemiology and End Results program of the National Cancer Institute in the United States from 1988 to 2011. A total of 49439 patients diagnosed with primary rectal cancer who underwent surgery were included. Clinicopathological characteristics and rectal cancer-specific survival between surgery alone group and surgery plus preoperative radiation therapy group were compared. Rectal cancer patients in surgery plus preoperative r...
Source: Neoplasma - July 12, 2019 Category: Cancer & Oncology Authors: Wang R, Zhao D, Liu YJ, Ye C, Qian JR, Dai JN, Liu SY, Liu JY, Li B, Wang MJ, Ping J Tags: Neoplasma Source Type: research

Accelerated atherosclerosis, hyperlipoproteinemia and insulin resistance in long-term survivors of Hodgkin lymphoma during childhood and adolescence.
Abstract Long-term survivors of Hodgkin lymphoma during childhood or adolescence (HL survivors) are at high risk of developing treatment-related late cardiovascular sequelae. In our study we evaluated the presence of modifiable cardiovascular risk factors (hypertension, hyperlipoproteinemia, hyperinsulinemia, obesity), endothelial and inflammatory markers (E-selectin, PAI-1, hs-CRP) and atherosclerotic changes in the common carotid arteries. Assessment was performed in 80 young adult Hodgkin lymphoma long-term survivors at more than 10 years after the potentially cardiovascular toxic anticancer treatment (median a...
Source: Neoplasma - July 12, 2019 Category: Cancer & Oncology Authors: Cepelova M, Kruseova J, Luks A, Capek V, Cepela P, Potockova J, Kraml P Tags: Neoplasma Source Type: research

Association between CA repeat polymorphism in IGF1 gene promoter and colorectal cancer risk in a native Chinese population.
In conclusion, our findings support the possible role of CA repeat polymorphism in CRC risk. PMID: 31305125 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - July 12, 2019 Category: Cancer & Oncology Authors: Chao XL, Wang LL, Liu R, Li Y, Zhou XJ Tags: Neoplasma Source Type: research

Three-dimensional conformal radiotherapy versus intensity modulated radiotherapy with simultaneous integrated boost in the treatment of locally advanced head and neck carcinoma.
Abstract The treatment of locally advanced head and neck cancer (LAHNC) requires a multimodality approach. Radiotherapy with combination of chemotherapy are demonstrated to be effective, however, the treatment intensification leads to increased toxicity at the same time. The implementation of three-dimensional conformal radiotherapy (3D-CRT) allowed to irradiate the treatment volume more precisely with better surrounding healthy tissue sparing. Intensity modulated radiotherapy (IMRT) facilitated higher conformity in dose shaping to target volume. IMRT with simultaneous integrated boost (IMRT-SIB) offered the possi...
Source: Neoplasma - June 19, 2019 Category: Cancer & Oncology Authors: Jirkovska M, Novak T, Malinova B, Lohynska R Tags: Neoplasma Source Type: research

Zoledronic acid modulates human osteosarcoma cells proliferation via GSK-3 β activation.
In this study, we found that the proliferation of MG-63 cells was significantly decreased after treatment with 25, 50, 100, or 200 μM ZOL for 48 and 72 h compared to that of untreated control cells. The expression levels of p-AKT/AKT and p-GSK-3β/GSK-3β in MG-63 cells and U-2 OS cells were inhibited by ZOL in both a dose- and time-dependent manner. Significant decreases in the expression of Cyclin D1, β-Catenin, and c-Myc were observed in the groups that underwent ZOL treatment. Additionally, compared to ZOL (100 μM) treatment alone, cotreatment with ZOL (100 μM) and Li2CO3 (1mM) rescued cell prolif...
Source: Neoplasma - June 17, 2019 Category: Cancer & Oncology Authors: Li S, Li JJ Tags: Neoplasma Source Type: research

Expression pattern of estrogen receptor β and its correlation with multidrug resistance in non-small cell lung cancer.
In conclusion, the results can be used to better understand the expression control of MDR1 and may allow for the establishment of new cancer chemistry strategies that will control P-gp expression in NSCLC. PMID: 31288527 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - June 17, 2019 Category: Cancer & Oncology Authors: Huang L, Liu T, Ji H, Yang S, Sui J, Yang WS, Liang GY, Zhang XM Tags: Neoplasma Source Type: research

FPR2 enhances colorectal cancer progression by promoting EMT process.
In conclusion our study demonstrated that FPR2 can promote CRC cells progression in vitro and in vivo that may relate to promoting EMT. PMID: 31288528 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - June 17, 2019 Category: Cancer & Oncology Authors: Lu J, Zhao J, Jia C, Zhou L, Cai Y, Ni J, Ma J, Zheng M, Lu A Tags: Neoplasma Source Type: research

Long non-coding RNA SNHG1 contributes to cisplatin resistance in non-small cell lung cancer by regulating miR-140-5p/Wnt/ β-catenin pathway.
Long non-coding RNA SNHG1 contributes to cisplatin resistance in non-small cell lung cancer by regulating miR-140-5p/Wnt/β-catenin pathway. Neoplasma. 2019 Jun 17;: Authors: Shi SL, Zhang ZH Abstract Small nucleolar RNA host gene 1 (SNHG1) has been identified to function as an oncogene in a large number of human cancers. Nevertheless, the biologic role and underlying molecular mechanism of SNHG1 on cisplatin (DDP)-resistance of NSCLC still unknown. qRT-PCR assay was performed to assess the expression levels of SNHG1 and miR-140-5p. Western blot analysis was used to determine Wnt1, cyclinD1, c-Myc...
Source: Neoplasma - June 17, 2019 Category: Cancer & Oncology Authors: Shi SL, Zhang ZH Tags: Neoplasma Source Type: research

The combination therapy of HIF1 α inhibitor LW6 and cisplatin plays an effective role on anti-tumor function in A549 cells.
This study revealed that hypoxia-improved cisplatin chemoresistance of NSCLC cells by regulated MDR1 and MRP1 expression via HIF1α/ROS pathway is reversed by LW6, suggesting that LW6 may act as effective sensitizer in chemotherapy for NSCLC. PMID: 31167534 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - June 3, 2019 Category: Cancer & Oncology Authors: Mai L, Luo M, Wu JJ, Yang JH, Hong LY Tags: Neoplasma Source Type: research

MiR-21-5p enhances the progression and paclitaxel resistance in drug-resistant breast cancer cell lines by targeting PDCD4.
Abstract MiR-21-5p has been identified as an oncogene to enhance human tumor progression. Here, we explored the mechanism by which miR-21-5p regulated the progression and paclitaxel (PTX) resistance in drug-resistant breast cancer (BC) cell lines. qRT-PCR assays were used to assess the expression levels of miR-21-5p and PDCD4 mRNA, and western blotting was used to detect PDCD4 protein level in PTX-resistant BC cell lines. Dual-luciferase reporter assay was used to observe the interaction between miR-21-5p and PDCD4 in PTX-resistant BC cell lines. Cell proliferation ability and IC50 values of PTX were measured by C...
Source: Neoplasma - June 3, 2019 Category: Cancer & Oncology Authors: Tao L, Wu YQ, Zhang SP Tags: Neoplasma Source Type: research

Analysis of ceRNA network identifies prognostic circRNA biomarkers in bladder cancer.
This study investigates if these circRNAs can be used as biomarkers for bladder cancer diagnosis. Using bioinformatics method to analysis GEO databases (GSE37815, GSE39093, GSE97239, and GSE92675) for differentially expressed RNAs in bladder cancer and normal bladder tissues were screened from. The related volcanic maps and the interaction network maps of differentially expressed RNAs were drawn, and the mRNA-miRNA and miRNA-circRNA interaction were predicted to establish mRNA-miRNA-circRNA competitive endogenous RNA (ceRNA) network. The differential circRNAs related to prognosis of bladder cancer patients were screened ba...
Source: Neoplasma - June 3, 2019 Category: Cancer & Oncology Authors: Liu L, Wu SQ, Zhu X, Xu R, Ai K, Zhang L, Zhao XK Tags: Neoplasma Source Type: research

The combination therapy of HIF1 α inhibitor LW6 and cisplatin plays an effective role on anti-tumor function in A549 cells.
This study revealed that hypoxia-improved cisplatin chemoresistance of NSCLC cells by regulated MDR1 and MRP1 expression via HIF1α/ROS pathway is reversed by LW6, suggesting that LW6 may act as effective sensitizer in chemotherapy for NSCLC. PMID: 31169018 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - June 3, 2019 Category: Cancer & Oncology Authors: Mai L, Luo M, Wu JJ, Yang JH, Hong LY Tags: Neoplasma Source Type: research

MiR-21-5p enhances the progression and paclitaxel resistance in drug-resistant breast cancer cell lines by targeting PDCD4.
Abstract MiR-21-5p has been identified as an oncogene to enhance human tumor progression. Here, we explored the mechanism by which miR-21-5p regulated the progression and paclitaxel (PTX) resistance in drug-resistant breast cancer (BC) cell lines. qRT-PCR assays were used to assess the expression levels of miR-21-5p and PDCD4 mRNA, and western blotting was used to detect PDCD4 protein level in PTX-resistant BC cell lines. Dual-luciferase reporter assay was used to observe the interaction between miR-21-5p and PDCD4 in PTX-resistant BC cell lines. Cell proliferation ability and IC50 values of PTX were measured by C...
Source: Neoplasma - June 3, 2019 Category: Cancer & Oncology Authors: Tao L, Wu YQ, Zhang SP Tags: Neoplasma Source Type: research

Analysis of ceRNA network identifies prognostic circRNA biomarkers in bladder cancer.
This study investigates if these circRNAs can be used as biomarkers for bladder cancer diagnosis. Using bioinformatics method to analysis GEO databases (GSE37815, GSE39093, GSE97239, and GSE92675) for differentially expressed RNAs in bladder cancer and normal bladder tissues were screened from. The related volcanic maps and the interaction network maps of differentially expressed RNAs were drawn, and the mRNA-miRNA and miRNA-circRNA interaction were predicted to establish mRNA-miRNA-circRNA competitive endogenous RNA (ceRNA) network. The differential circRNAs related to prognosis of bladder cancer patients were screened ba...
Source: Neoplasma - June 3, 2019 Category: Cancer & Oncology Authors: Liu L, Wu SQ, Zhu X, Xu R, Ai K, Zhang L, Zhao XK Tags: Neoplasma Source Type: research

Identification of key pathways and genes changes in pancreatic cancer cells (BXPC-3) after cross-talked with primary pancreatic stellate cells using bioinformatics analysis.
In conclusion, the present study indicated that the identified DEGs promote our understanding of the molecular mechanisms underlying the interaction between pancreatic cancer cells and PSCs and might be used as molecular targets in the future to study the role of tumor microenvironment in the progression of PDAC. PMID: 31167532 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - June 2, 2019 Category: Cancer & Oncology Authors: Tang D, Wu Q, Yuan Z, Xu J, Zhang H, Jin Z, Zhang Q, Xu M, Wang Z, Dai Z, Fang H, Li Z, Lin C, Shi C, Xu M, Sun X, Wang D Tags: Neoplasma Source Type: research

Differential expression of Sonic Hedgehog in lung adenocarcinoma and lung squamous cell carcinoma.
Abstract Overexpression of Sonic hedgehog (Shh) is associated with progression of several cancers. The expression of Shh in non-small cell lung cancer (NSCLC) has been reported with inconsistent results. Lung adenocarcinoma (LAC) and lung squamous cell carcinoma (LSCC) are two major subtypes of NSCLC, which have different genetic genotypes and clinical therapeutic options. The expression of Shh in specimen of patients with NSCLC has yet to be comprehensively determined according to histological subtypes. Shh expression level was determined in 167 NSCLC patients (56 LAC patients and 111 LSCC patients) by immunohist...
Source: Neoplasma - June 2, 2019 Category: Cancer & Oncology Authors: Xu L, Xiong H, Shi W, Zhou F, Zhang M, Hu G, Mei J, Luo S, Chen L Tags: Neoplasma Source Type: research

Identification of key pathways and genes changes in pancreatic cancer cells (BXPC-3) after cross-talked with primary pancreatic stellate cells using bioinformatics analysis.
In conclusion, the present study indicated that the identified DEGs promote our understanding of the molecular mechanisms underlying the interaction between pancreatic cancer cells and PSCs and might be used as molecular targets in the future to study the role of tumor microenvironment in the progression of PDAC. PMID: 31169017 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - June 2, 2019 Category: Cancer & Oncology Authors: Tang D, Wu Q, Yuan Z, Xu J, Zhang H, Jin Z, Zhang Q, Xu M, Wang Z, Dai Z, Fang H, Li Z, Lin C, Shi C, Xu M, Sun X, Wang D Tags: Neoplasma Source Type: research

Receptor interacting protein kinase 3 promotes cisplatin-induced necroptosis in apoptosis-resistant HepG2/DDP cells.
In this study, we provided evidence that cisplatin could induce cell death in HepG2 cells, but not in the apoptosis-resistant HepG2/DDP cells. Ectopic expression of RIP3 promoted cisplatin-induced HepG2/DDP cells death, HMGB1 and LDH release. Moreover, we demonstrated this type of cell death was necroptosis and dependent RIP1-RIP3-MLKL signaling pathway because inhibition of MLKL activity by necrosulfonamide (NSA) or knockdown of RIP1 significantly attenuated cisplatin-induced cell death in HepG2/DDP-RIP3 cells. Finally, we found that ectopic expression of RIP3 sensitized HepG2/DDP cancer cells to cisplatin treatment in vi...
Source: Neoplasma - May 11, 2019 Category: Cancer & Oncology Authors: Zhang B, Cao K, Liu Z, Shan W, Wen Q, Wang R Tags: Neoplasma Source Type: research

Association between p53 protein phosphorylated at serine 20 expression and ovarian carcinoma stem cells phenotype: correlation with clinicopathological parameters of ovarian cancer.
We examined correlation between p53 protein phosphorylated at serine 20 (p-p53(Ser20)) and CD133, SOX2, Notch1 expression in order to reveal p-p53(Ser20) stemness function in ovarian cancer. p-p53(Ser20), CD133, Notch1, SOX2 expression was analyzed on 104 ovarian carcinomas using immunohistochemical staining. The positive correlation between p53 and p-p53(Ser20) (P=0.02), p53 and SOX2 (P=0.02), p-p53(Ser20) and Notch1 (P=0.03), p-p53(Ser20) and CD133 (P=0.01) expression was observed in ovarian carcinomas. The parallel expression of p-p53(Ser20)/CD133, p-p53(Ser20)/Notch1 reflecting coexpression of these proteins in single ...
Source: Neoplasma - May 11, 2019 Category: Cancer & Oncology Authors: Bar J, Grelewski P, Deszcz I, Noga L, Hirnle L, Lis-Nawara A Tags: Neoplasma Source Type: research

MicroRNA-107 inhibits proliferation of prostate cancer cells by targeting cyclin E1.
In conclusion, miR-107 could be a potential tumor suppressor in PCa, and the restoration of miR-107 might provide a new therapeutic option for PCa. PMID: 31088102 [PubMed - in process] (Source: Neoplasma)
Source: Neoplasma - May 11, 2019 Category: Cancer & Oncology Authors: Zhang X, Jin K, Luo JD, Liu B, Xie LP Tags: Neoplasma Source Type: research

Circulating miR-31-5p may be a potential diagnostic biomarker in nasopharyngeal carcinoma.
Abstract Aberrant expression of miR-31-5p has been detected in various cancers and plays a significant role in tumourigenesis. Low miR-31-5p expression was present in nasopharyngeal carcinoma (NPC) tissues and cell lines and acted as a tumour suppressive miRNA. Currently, circulating miRNAs are emerging as novel biomarkers for the early diagnosis of cancers using a non-invasive method. However, circulating miR-31-5p has rarely been reported in NPC. Therefore, the aim of this study was to explore peripheral blood miR-31-5p levels as a noninvasive biomarker and evaluate its clinical value for the early diagnosis of ...
Source: Neoplasma - May 11, 2019 Category: Cancer & Oncology Authors: Yi SJ, Liu P, Chen BL, Ou-Yang L, Xiong WM, Su JP Tags: Neoplasma Source Type: research

The effect of postoperative radiotherapy on the survival of patients with resectable stage III-N2 non-small-cell lung cancer: a systematic review and meta-analysis.
Abstract In the potentially resectable cases of stage III-N2 non-small-cell lung cancer (NSCLC), the optimal postoperative treatment regimen for these patients is uncertain, and post-operative radiation therapy (PORT) with chemotherapy is typically recommended. Our aim was to reassess the data of PORT on overall survival (OS) and disease-free survival (DFS) in stage III-N2 NSCLC, in order to figure out whether PORT might lead to a moderate improvement in local control and survival besides resection and adjuvant chemotherapy. A comprehensive search strategy was performed in EMBASE, PubMed, and Cochrane Library for ...
Source: Neoplasma - May 11, 2019 Category: Cancer & Oncology Authors: Zhang H, Zhang DX, Ju T, Zhou J Tags: Neoplasma Source Type: research

Diagnosis and treatment of juvenile myelomonocytic leukemia in Slovak Republic: novel approaches.
We present 6 patients with JMML, harboring different somatic mutations (PTPN11 and NRAS), with distinct clinical features; 3 of them had been treated with AZA 75mg/m2 i.v. on days 1 to 7 of a 28-day cycle before the HSCT. Response to therapy was evaluated after each cycle in accordance with the International response criteria. One patient had a progression of splenomegaly during the treatment and after three cycles he was urgently transplanted. At present, he is remaining in complete remission 3 years after HSCT. Two patients showed impressive response following the first cycle of the therapy with a regression of splenomeg...
Source: Neoplasma - May 11, 2019 Category: Cancer & Oncology Authors: Fabri O, Horakova J, Bodova I, Svec P, Laluhova Striezencova Z, Bubanska E, Cermak M, Galisova V, Skalicka K, Vaska A, Doczyova D, Panikova A, Sykora T, Adamcakova J, Kolenova A Tags: Neoplasma Source Type: research

TRIM31 promotes proliferation, invasion and migration of glioma cells through Akt signaling pathway.
This study is intended to investigate the role of Tripartite Motif (TRIM) 31 in glioma. Immunohistochemistry and Western blot analysis showed that TRIM31 was overexpressed in high-grade glioma tissues. Univariate survival analysis indicated that high expression of TRIM31 was related to short survival time of glioma patients. Multivariate survival analysis demonstrated that TRIM31 was an independent prognostic factor for glioma patients. In addition, through the experiments on glioma cell lines, we found that after silencing or overexpressing TRIM31 expression, the proliferation, invasion and migration of glioma cells could...
Source: Neoplasma - May 11, 2019 Category: Cancer & Oncology Authors: Shi G, Lv C, Yang Z, Qin T, Sun L, Pan P, Wang D Tags: Neoplasma Source Type: research

The prognostic value of the 8th edition of the American Joint Committee on Cancer (AJCC) staging system in triple-negative breast cancer.
This study aims to compare anatomic staging (AS) with prognostic staging (PS) based on the updated AJCC 8th edition staging manual. A retrospective single-center analysis of 313 triple-negative breast invasive ductal carcinoma patients who received surgery at department of breast surgery in the Fourth Hospital of Hebei Medical University from 2010-01 to 2012-12 was performed. All cases were restaged using the AJCC 8th edition AS and PS system. The 7-year DFS and the 7-year OS rates were 76.30% and 78.27%, respectively. Applying the PS system, 277 (88.5%) patients of the AS groups were up-staged to the PS groups, 31 cases w...
Source: Neoplasma - May 11, 2019 Category: Cancer & Oncology Authors: Li JP, Zhang XM, Zhang YS, Zheng LH, Liu YJ Tags: Neoplasma Source Type: research

The value of detecting pepsinogen and gastrin-17 levels in serum for pre-cancerous lesion screening in gastric cancer.
Abstract The aim of the study was to estimate the value of detecting pepsinogen (PG) I, PGII, and gastrin-17 (G-17) levels in serum for chronic atrophic gastritis (CAG) screening and to determine the clinical applicability of combined measurement of serum G-17, pepsinogens (PGI, PGII) and PGI/PGII ratio (PGR) as a screening test for CAG. The PGI, PGII, and G-17 levels were detected by ELISA in 68 patients with CAG and 86 healthy volunteers who underwent gastroscopy for gastroduodenal diseases at Taizhou Municipal Hospital between January 2016 and December 2016. Concentrations of all measured serum markers were low...
Source: Neoplasma - April 24, 2019 Category: Cancer & Oncology Authors: Yu G, Wang GX, Wang HG, Mo FF, Tang BB Tags: Neoplasma Source Type: research

Cucurbitacin B inhibits cell proliferation and induces cell apoptosis in colorectal cancer by modulating methylation status of BTG3.
Abstract A previous report has revealed that cucurbitacin B (CuB) inhibits cancer cell proliferation and tumorigenesis in non-small cell lung cancer (NSCLC) through epigenetic modifications of several genes. However, whether CuB regulates cell proliferation and apoptosis by altering methylation status of BTG3 in colorectal cancer (CRC) remains unknown. In the present study, the results showed that BTG3 was downregulated in CRC tissues compared with adjacent normal tissues. CuB significantly increased BTG3 levels, induced promoter demethylation, and decreased the levels of DNA methyltransferases (DNMT1, DNMT3a and ...
Source: Neoplasma - April 24, 2019 Category: Cancer & Oncology Authors: Mao D, Liu AH, Wang ZP, Zhang XW, Lu H Tags: Neoplasma Source Type: research

Clinical and prognostic significance of BRAF V600E mutation in non-metastatic cutaneous melanoma patients.
In conclusion, there is no prognostic value of BRAF V600E mutation on overall survival in stage I-III melanoma patients, yet its presence might indicate a decreased risk for development of relapse and/or metastasis in stage III melanoma patients. PMID: 31058533 [PubMed - as supplied by publisher] (Source: Neoplasma)
Source: Neoplasma - April 24, 2019 Category: Cancer & Oncology Authors: Tas F, Erturk K Tags: Neoplasma Source Type: research

Effects of neuroblastoma breakpoint family member 1 (NBPF1) gene on growth and Akt-p53-Cyclin D pathway in cutaneous squamous carcinoma cells.
This study mainly explored the expression and mechanism of NBPF1 in SCC. SCC tissue and adjacent tissues samples were randomly selected. NBPF1 gene was overexpressed in the A431 cell line using plasmid transfection technique. Cell viability was tested by cell counting kit-8 (CCK-8) assay. Flow cytometry was used to determine cell cycle and apoptosis. Western blot and RT-qPCR were respectively performed to determine the expression levels of proteins and mRNAs. The NBPF1 gene was lowly expressed in SCC tissues. The expression level of NBPF1 gene was the lowest in A431 cell line. The cell viability of A431 was reduced after t...
Source: Neoplasma - April 24, 2019 Category: Cancer & Oncology Authors: Gao Y, Zhu H, Mao Q Tags: Neoplasma Source Type: research