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A sensitive NIR mitochondria-targeting fluorescence probe for visualizing viscosity in living cells and mice
Anal Chim Acta. 2022 Oct 23;1231:340443. doi: 10.1016/j.aca.2022.340443. Epub 2022 Sep 27.ABSTRACTMitochondria are the powerhouses in cells, providing the energy needed for cellular activities. However, the abnormalities in the mitochondrial microenvironment (e.g., the increased viscosity) can lead to mitochondrial dysfunctions and diseases. Herein, we develop a series of near-infrared (NIR) fluorescence probes for the detection of viscosity. After screening, probe CQ-4 is selected since it shows a great fluorescence enhancement (89-fold) in the NIR window. Its specific response to viscosity is not influenced by pH, polari...
Source: Analytica Chimica Acta - October 11, 2022 Category: Chemistry Authors: Qian Chen Xiao-Fan Zhang Teng Wang Xiao-Qun Cao Shi-Li Shen Source Type: research

Mitochondria targeted dual-fluorescent probe for bio-imaging viscosity and F - with different fluorescence signals
Bioorg Chem. 2022 Sep 23;129:106169. doi: 10.1016/j.bioorg.2022.106169. Online ahead of print.ABSTRACTThe F- ion and viscosity both affect the physiological state of mitochondria and to the best of our knowledge no fluorescent probe is reported for the dual detection of mitochondrial viscosity and F- ion through different signals. DMAS-Si is weakly red fluorescent due to free intramolecular rotation between dimethylaminophenyl and pyridinium moieties and PET from silyloxy to the pyridinium moiety. In viscous medium (glycerol 90 %), the rotation is restricted and 18-fold increase in red-fluorescence (λem 637 nm) is observe...
Source: Bioorganic Chemistry - September 29, 2022 Category: Chemistry Authors: Sukhvinder Dhiman Rasdeep Kour Satwinderjeet Kaur Prabhpreet Singh Subodh Kumar Source Type: research

Amphotericin B resistance in Leishmania mexicana : Alterations to sterol metabolism and oxidative stress response
by Edubiel A. Alpizar-Sosa, Nur Raihana Binti Ithnin, Wenbin Wei, Andrew W. Pountain, Stefan K. Weidt, Anne M. Donachie, Ryan Ritchie, Emily A. Dickie, Richard J. S. Burchmore, Paul W. Denny, Michael P. Barrett Amphotericin B is increasingly used in treatment of leishmaniasis. Here, fourteen independent lines ofLeishmania mexicana and oneL.infantum line were selected for resistance to either amphotericin B or the related polyene antimicrobial, nystatin. Sterol profiling revealed that, in each resistant line, the predominant wild-type sterol, ergosta-5,7,24-trienol, was replaced by other sterol intermediates. Broadly, two ...
Source: PLoS Neglected Tropical Diseases - September 28, 2022 Category: Tropical Medicine Authors: Edubiel A. Alpizar-Sosa Source Type: research

Resistance profiles to antifungal agents in Candida albicans isolated from human oral cavities: systematic review and meta-analysis
ConclusionsClinicalC. albicans isolates had low antifungal resistance.Clinical relevanceThe presence of concomitant systemic comorbidities appears to be an essential factor that should be considered when evaluating resistance to antifungals for oral isolates. (Source: Clinical Oral Investigations)
Source: Clinical Oral Investigations - September 27, 2022 Category: Dentistry Source Type: research

A new source of bacterial myrosinase isolated from endophytic Bacillus sp. NGB-B10, and its relevance in biological control activity
This study provides original information about a new source of bacterial m yrosinase and affords an optimized method to enhance myrosinase production. (Source: World Journal of Microbiology and Biotechnology)
Source: World Journal of Microbiology and Biotechnology - September 3, 2022 Category: Microbiology Source Type: research

Giardial lipid rafts share virulence factors with secreted vesicles and participate in parasitic infection in mice
Giardia lamblia, a protozoan parasite, is a major cause of waterborne infection, worldwide. While the trophozoite form of this parasite induces pathological symptoms in the gut, the cyst form transmits the infection. Since Giardia is a noninvasive parasite, the actual mechanism by which it causes disease remains elusive. We have previously reported that Giardia assembles cholesterol and GM1 glycosphingolipid-enriched lipid rafts (LRs) that participate in encystation and cyst production. To further delineate the role of LRs in pathogenesis, we isolated LRs from Giardia and subjected them to proteomic analysis. Various cellu...
Source: Frontiers in cellular and infection microbiology - August 23, 2022 Category: Microbiology Source Type: research

FXYD3 facilitates Na + and liquid absorption across human airway epithelia by increasing the transport capacity of the Na/K ATPase
Am J Physiol Cell Physiol. 2022 Aug 22. doi: 10.1152/ajpcell.00047.2022. Online ahead of print.ABSTRACTNa/K ATPase activity is essential for ion transport across epithelia. FXYD3, a ᵯE; subunit of the Na/K ATPase, is expressed in the airway, but its function remains undetermined. Single cell RNA sequencing and immunohistochemistry revealed that FXYD3 localizes within the basolateral membrane of all airway epithelial cells. To study FXYD3 function, we reduced FXYD3 expression using siRNA. After permeabilizing the apical membrane with nystatin, epithelia pretreated with FXYD3-targeting siRNA had lower ouabain-sensitive sho...
Source: Am J Physiol Cell Ph... - August 22, 2022 Category: Cytology Authors: Camilo Cano Portillo Raul Villacreses Andrew L Thurman Alejandro Antonio Pezzulo Joseph Zabner Ian M Thornell Source Type: research

FXYD3 facilitates Na + and liquid absorption across human airway epithelia by increasing the transport capacity of the Na/K ATPase
Am J Physiol Cell Physiol. 2022 Aug 22. doi: 10.1152/ajpcell.00047.2022. Online ahead of print.ABSTRACTNa/K ATPase activity is essential for ion transport across epithelia. FXYD3, a ᵯE; subunit of the Na/K ATPase, is expressed in the airway, but its function remains undetermined. Single cell RNA sequencing and immunohistochemistry revealed that FXYD3 localizes within the basolateral membrane of all airway epithelial cells. To study FXYD3 function, we reduced FXYD3 expression using siRNA. After permeabilizing the apical membrane with nystatin, epithelia pretreated with FXYD3-targeting siRNA had lower ouabain-sensitive sho...
Source: American Journal of Physiology. Cell Physiology - August 22, 2022 Category: Cytology Authors: Camilo Cano Portillo Raul Villacreses Andrew L Thurman Alejandro Antonio Pezzulo Joseph Zabner Ian M Thornell Source Type: research