Antifungal Activity of ZnO Nanoparticles and Nystatin and Downregulation of SAP1-3 Genes Expression in Fluconazole-Resistant Candida albicans Isolates from Vulvovaginal Candidiasis
(Source: Infection and Drug Resistance)
Source: Infection and Drug Resistance - February 12, 2020 Category: Infectious Diseases Tags: Infection and Drug Resistance Source Type: research

Molecules, Vol. 25, Pages 790: Design, Synthesis, and Antimicrobial Activities of 1,2,3-Triazole Glycoside Clickamers
El-Sayed Bacterial resistance remains a significant threat and a leading cause of death worldwide, despite massive attempts to control infections. In an effort to develop biologically active antibacterial and antifungal agents, six novel aryl-substituted-1,2,3-triazoles linked to carbohydrate units were synthesized through the Cu(I)-catalyzed azide-alkyne cycloaddition CuAAC of substituted-arylazides with a selection of alkyne-functionalized sugars. The chemical structures of the new derivatives were verified using different spectroscopic techniques. The novel clicked 1,2,3-triazoles were evaluated for in vitro antiba...
Source: Molecules - February 12, 2020 Category: Chemistry Authors: Tamer El Malah Hany F. Nour Amira A. E. Satti Bahaa A. Hemdan Wael A. El-Sayed Tags: Article Source Type: research

The antimicrobial and antibiofilm properties of allicin against Candida albicans and Staphylococcus aureus – A therapeutic potential for denture stomatitis
The objective of this study is to determine the therapeutic efficacy of allicin against Candida albicans (C. albicans) and Staphylococcus aureus (S. aureus), the common etiological agents for denture stomatitis (DS). The minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentration (MFCs) of allicin were determined by the broth microdilution method followed by checkerboard microdilution method for a synergistic interaction between allicin+nystatin and allicin+CHX. The potential of allicin to eradicate C. albicans and S. aureus biofilms was assessed by treating biof...
Source: The Saudi Dental Journal - February 7, 2020 Category: Dentistry Source Type: research

Molecular modeling study of antibacterial molecules on nylon 6,6 surfaces
Publication date: Available online 6 February 2020Source: Materials Today: ProceedingsAuthor(s): Adam Verner, Jonáš TokarskýAbstractPresent study is focused on the molecular modeling of various compounds serving as antibacterial agents – nystatin (NYS), chlorhexidine (CH), and dodecyltrimethylammonium bromide (DTAB) – on ideal and imperfect, i.e. containing surface defects, (1 0 0) and (0 1 0) surfaces of nylon 6,6 nanofibers. The study was performed in the Materials Studio/Forcite modeling environment using COMPASS force field. After successful validation of modeling strategy, inte...
Source: Materials Today: Proceedings - February 7, 2020 Category: Materials Science Source Type: research

A Holistic Approach to Diagnosing and Treating Dysphagia
We report a 39-year-old Native American female with an almost 20-year history of dysphagia that had increased in the 6  months prior to the initial evaluation. Investigation revealed a number of distinct esophageal disorders including Plummer–Vinson syndrome, gastroesophageal reflux disease with esophagitis, distal esophageal stricture, esophageal intramural pseudo-diverticulosis, and recurrent esophageal Candida infections. Although prolonged therapy with proton pump inhibitors, fluconazole, nystatin, and repeated esophageal balloon dilations relieved her symptoms, her prognosis remains uncertain. (Source: Dige...
Source: Digestive Diseases and Sciences - February 5, 2020 Category: Gastroenterology Source Type: research

Copper IUD increases virulence of non-albicans Candida species isolated from women with vulvovaginal candidiasis.
Abstract Objectives: The aim of the study was to investigate the effect of copper-bearing intrauterine device (IUD) use on the virulence of Candida species causing vulvovaginal candidiasis (VVC). The in vitro ability of Candida species to produce proteinase and phospholipase enzymes was studied, together with their antifungal susceptibility.Methods: Vaginal swabs from women with VVC were cultured and Candida species were identified. Participants comprised 132 women with culture-confirmed VVC, of whom 65 were using a copper-bearing IUD and 67 were not. Candida isolates were tested for their ability to produce ...
Source: The European Journal of Contraception and Reproductive Health Care - January 28, 2020 Category: Reproduction Medicine Authors: Omran EA, Youssef NES, Abdelfattah AH, Esmail SA, Fouad AM Tags: Eur J Contracept Reprod Health Care Source Type: research

Generation of tetramycin B derivative with improved pharmacological property based on pathway engineering.
Abstract Polyene antibiotics, including amphotericin, nystatin, pimaricin, and tetramycin, are important antifungal agents. Increasing the production of polyenes and generation of their improved analogues based on the biosynthetic pathway engineering has aroused wide concern in application researches. Herein, tetramycin and nystatin, both of which share most of acyl-CoA precursors, are produced by Streptomyces hygrospinosus var. beijingensis CGMCC 4.1123. Thus, the intracellular malonyl-CoA is found to be insufficient for PKSs (polyketide synthases) extension of tetramycin by quantitative analysis in this wild-typ...
Source: Applied Microbiology and Biotechnology - January 27, 2020 Category: Microbiology Authors: Sheng Y, Ou Y, Hu X, Deng Z, Bai L, Kang Q Tags: Appl Microbiol Biotechnol Source Type: research

Stimulated Biosynthesis of an C10-Deoxy Heptaene NPP B2 via Regulatory Genes Overexpression in Pseudonocardia autotrophica
In this study, another novel derivative called NPP B2 was developed, which lacks a hydroxyl group at the C10 position by site-specific gene disruption of the P450 hydroxylase NppL. To stimulate the extremely low expression of the NPP B2 biosynthetic pathway genes, the 32-kb NPP-specific regulatory gene cluster was overexpressed via site-specific chromosomal integration. The extra copy of the six NPP-specific regulatory genes led to a significant increase in the NPP B2 yield from 0.19 to 7.67 mg/L, which is the highest level of NPP B2 production ever achieved by the P. autotrophica strain. Subsequent in vitro antifungal act...
Source: Frontiers in Microbiology - January 24, 2020 Category: Microbiology Source Type: research

Glutaryl melatonin niosome gel for topical oral mucositis: anti-inflammatory and anticandidiasis.
Abstract BACKGROUND: Glutaryl melatonin, which is synthesized from melatonin and is a pineal gland-derived neurohormone with anti-inflammatory and anti-oxidant properties, was comparatively investigated for its potential use as a topical anti-inflammatory agent. OBJECTIVE: Glutaryl melatonin, synthesized and screened for in vitro anti-candidiasis and in vitro and in vivo anti-inflammatory activities, was formulated as a niosome gel for topical oral evaluation in 5-fluorouracil-induced oral mucositis in mice. METHOD: In vitro anti-fungal activity in Candida albicans, in vitro anti-inflammatory activity in...
Source: Current Drug Delivery - January 22, 2020 Category: Drugs & Pharmacology Authors: Damrongrungruang T, Panpitakkul P, Somudorn J, Saengchart P, Mahakunakorn P, Uthaiwat P, Daduang J, Panyatip P, Puthongking P, Priprem A Tags: Curr Drug Deliv Source Type: research

Effectiveness and pharmacokinetic evaluation of GEoNIstin ® (oxytetracyline and nystatin) vaginal tablEts for unspecific and mixed vulvovaginal infections (GENIE study)
The GENIE study was performed to evaluate the effectiveness and systemic exposure to oxytetracycline in local treatment of unspecific and mixed vulvovaginal infections characterized by vaginal discharge with Geonistin® vaginal tablets (100 mg oxytetracycline and 100 000 IU nystatin). The total number of subjects enrolled was 189. The treatment had beneficial effects in 100% of the study population. According to the Nugent score, the treatment had a positive effect in 89.2% of participants. The microbiological cure rate was 78.8%. Oxytetracycline concentration levels were from 13.3 to 32.2 ng/mL in 11 out of 15 subjects...
Source: Acta Dermatovenerologica Croatica - January 17, 2020 Category: Dermatology Source Type: research

Enhancement of metabolic flux toward ε-poly-l-lysine biosynthesis by targeted inactivation of concomitant polyene macrolide biosynthesis in Streptomyces albulus
Publication date: Available online 8 January 2020Source: Journal of Bioscience and BioengineeringAuthor(s): Kazuya Yamanaka, Yoshimitsu Hamano, Tadao Oikawaε-Poly-l-lysine (ε-PL) produced as a secondary metabolite of Streptomyces albulus has long been used as a natural food preservative in a number of countries, including Japan, the United States, South Korea, and China. To date, numerous studies employing classical biotechnological approaches have been carried out to improve its productivity. Here we report a modern and rational genetic approach to enhancing metabolic flux toward ε-PL biosynthesis....
Source: Journal of Bioscience and Bioengineering - January 8, 2020 Category: Biomedical Science Source Type: research

In vitro antifungal resistance profile of Candida strains isolated from Saudi women suffering from vulvovaginitis
ConclusionThe emergence of resistantCandida strains necessitates conduction of the antifungal susceptibility test prior to deciding the medication regime. (Source: European Journal of Medical Research)
Source: European Journal of Medical Research - January 4, 2020 Category: Research Source Type: research

Synthesis, Antimicrobial and Antitumor Evaluations of a New Class of Thiazoles Substituted on the Chromene Scaffold.
Abstract BACKGROUND & OBJECTIVE: A new series of thiazoles substituted on the chromene scaffold were prepared by facial approaches starting from (E)-1-(2,3-Dihydrochromen-4-ylidene)thiosemicarbazide derivatives (2a,b). The thiosemicarbazides (2a,b) were reacted with a series of α-halo carbonyl compounds to give the corresponding rhodanine analogues and reacted also with C-acetyl-or Cethoxy- N-hydrazonoyl chlorides to afford the corresponding tri- and tetra-substituted hybrid hydrazinyl thiazole substituted chromenes. METHODS: The newly synthesized compounds were screened for their in vitro antimicro...
Source: Mini Reviews in Medicinal Chemistry - December 28, 2019 Category: Chemistry Authors: Elnaggar DH, Abdel Hafez NA, Rashdan HRM, Abdelwahed NAM, Awad HM, Ali KA Tags: Mini Rev Med Chem Source Type: research

Venlafaxine chloridrate-oral lichenoid reaction: case report
Venlafaxine hydrochloride is a structurally novel antidepressant that inhibits reuptake of 5-hydroxytryptamine and noradrenaline but, unlike the older antidepressants, has few adverse effects. A 57-year-old female patient, a smoker with anxiety, on psychiatric treatment with venlafaxine hydrochloride attended our oral medicine unit complaining of a white spot on the right cheek with 1 year since the onset, which did not regress after the use of nystatin and triamcinolone acetonide. The intraoral clinical examination revealed the presence of white striae and leucoplastic plaque on the buccal mucosa and on the right lateral ...

Exchange of water for sterol underlies sterol egress from a StARkin domain
Previously we identified Lam/GramD1 proteins, a family of endoplasmic reticulum membrane proteins with sterol-binding StARkin domains (Gatta et al. eLife 2015), which are implicated in intracellular sterol homeostasis. Here we show how these proteins exchange sterol molecules with membranes. An aperture at one end of the StARkin domain enables sterol to enter/exit the binding pocket. Strikingly, the wall of the pocket is longitudinally fractured, exposing bound sterol to solvent. Large-scale atomistic molecular dynamics simulations reveal that sterol egress involves widening of the fracture, penetration of water into the c...
Source: eLife - December 4, 2019 Category: Biomedical Science Tags: Biochemistry and Chemical Biology Source Type: research

RAFT-Derived Polymethacrylates as a Superior Treatment for Recurrent Vulvovaginal Candidiasis by Targeting Biotic Biofilms and Persister Cells
ConclusionThis study systematically assessed the clinical potential of RAFT-derived polymethacrylates as an effective treatment for VVC/RVVC in a mouse model. Polymethacrylates effectively killed vaginal epithelium-related C. albicans in vivo by specially targeting biotic biofilms and persister cells. Treatment presented negligible local toxicity. (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - November 7, 2019 Category: Microbiology Source Type: research

Spiroplasma eriocheiris Enters Drosophila Schneider 2 Cells and Relies on Clathrin-Mediated Endocytosis and Macropinocytosis [Bacterial Infections]
Spiroplasma eriocheiris causes great economic losses in the crustacean aquaculture industry. However, the mechanism of S. eriocheiris infecting host cells has been poorly studied. We established a Spiroplasma-infected Drosophila Schneider 2 (S2) cell model and investigated its pathogenic mechanism. First, S. eriocheiris induced S2 cell apoptosis and necrosis, seriously decreased cell viability, and increased the production of intracellular reactive oxygen species. Further research showed that S. eriocheiris can invade S2 cells, and the number of copies of intracellular spiroplasmas is sharply increased by 12 h postinfectio...
Source: Infection and Immunity - October 18, 2019 Category: Infectious Diseases Authors: Wei, P., Ning, M., Yuan, M., Li, X., Shi, H., Gu, W., Wang, W., Meng, Q. Tags: Bacterial Infections Source Type: research

Flavonoids, antioxidant potential and antimicrobial activity of Myrcia rufipila mcvaugh leaves (myrtaceae).
This study shows that M. rufipila, like other Myrcia species, is another source of flavonoids such as desmantin-I and myricitrin which have shown hypoglycemic potential, besides triterpenes and phenolic acids. PMID: 31526144 [PubMed - as supplied by publisher] (Source: Natural Product Research)
Source: Natural Product Research - September 17, 2019 Category: Biochemistry Authors: Cascaes MM, Guilhon GMSP, Zoghbi MDG, Andrade EHA, Santos LS, Kelly R da Silva J, Trovatti Uetanabaro AP, Araújo IS Tags: Nat Prod Res Source Type: research

Synthesis, antimicrobial activity, and determination of the lipophilicity of ((cyclohex-3-enylmethylene)hydrazinyl)thiazole derivatives
AbstractSynthesis and investigation of antimicrobial activity of fifteen novel thiazoles containing cyclohexene moiety are presented. Among the derivatives, compounds3a–3d, 3f, 3n, and3o showed very strong activity against the referenceCandida spp. strains with MIC  = 0.015–3.91 µg/ml. The activity of these compounds is similar and even higher than the activity of nystatin used as positive control. Compounds3d,3f,3n,3o showed the highest activity with very strong effect towards most of yeasts isolated from clinical materials with MIC  = 0.015–7.81 µg/m...
Source: Medicinal Chemistry Research - September 9, 2019 Category: Chemistry Source Type: research

Nystatin complexation with β-cyclodextrin: Spectroscopic evaluation of inclusion by FT-Raman, photoacoustic spectroscopy, and 1H NMR
Publication date: 1 January 2020Source: Materials Chemistry and Physics, Volume 239Author(s): Aloisi Somer, João Ricardo Roik, Mauricio Aparecido Ribeiro, Amanda M. Urban, Amanda Schoeffel, Vanessa M. Urban, Paulo V. Farago, Lidiane Vizioli de Castro, Francielle Sato, Carlos Jacinto, Eliane Campesatto, Magna Suzana Alexandre Moreira, Andressa NovatskiAbstractThe inclusion complex of Nystatin (NYS) with β-cyclodextrin (β-CD) can increase the solubility of the antibiotic in aqueous media. The complexes were obtained by freeze-drying (FD) and spray-drying (SD) methods. The spectroscopic techniques used to ana...
Source: Materials Chemistry and Physics - September 6, 2019 Category: Materials Science Source Type: research

Correlating Genotype and Phenotype in the Asexual Yeast Candida orthopsilosis Implicates ZCF29 in Sensitivity to Caffeine
Candida orthopsilosis is diploid asexual yeast that causes human disease. Most C. orthopsilosis isolates arose from at least four separate hybridizations between related, but not identical, parents. Here, we used population genomics data to correlate genotypic and phenotypic variation in 28 C. orthopsilosis isolates. We used cosine similarity scores to identify 65 variants with potential high-impact (deleterious effects) that correlated with specific phenotypes. Of these, 19 were Single Nucleotide Polymorphisms (SNPs) that changed stop or start codons, or splice sites. One variant resulted in a premature stop codon in both...
Source: G3: Genes Genomes Genetics - September 4, 2019 Category: Genetics & Stem Cells Authors: de San Vicente, K. M., Schröder, M. S., Lombardi, L., Iracane, E., Butler, G. Tags: Investigations Source Type: research

In vitro activity of carvacrol, cinnamaldehyde and thymol combined with antifungals against Malassezia pachydermatis
In conclusion, the results obtained indicate that the phytochemicals tested showed relevant in vitro anti-M. pachydermatis activity. Future in vivo experiments are needed to elucidate the safety and therapeutic potential of these combinations.RésuméMalassezia pachydermatis est un agent opportuniste important de la dermatite et de l'otite chez le chien. M. pachydermatis est généralement traité au moyen de traitements topiques utilisant une combinaison d'agents antifongiques, antimicrobiens et anti-inflammatoires. Nous avons étudié les activités in vitro du carvacrol (C...
Source: Journal of Medical Mycology - August 20, 2019 Category: Biology Source Type: research

The comparison of the effect of curcumin with nystatin on inhibition level of Candida albicans
(Source: International Journal of Nanomedicine)
Source: International Journal of Nanomedicine - August 16, 2019 Category: Nanotechnology Tags: Journal of Experimental Pharmacology Source Type: research

Dual-detection of mitochondrial viscosity and SO2 derivatives with two cross-talk-free emissions employing a single two-photon fluorescent probe
Publication date: 15 October 2019Source: Sensors and Actuators B: Chemical, Volume 297Author(s): Guoshun Fang, Xiuli Yang, Wenjuan Wang, Yan Feng, Wan Zhang, Yinliang Huang, Chuan Sun, Man Chen, Xiangming MengAbstractA versatile two-photon fluorescent probe MCB for dual-detection of viscosity and SO2 derivatives in mitochondria with two cross-talk-free emissions (wavelength difference of 174 nm) has been rationally designed and synthesized. To our delight, probe MCB can not only recognize SO2 derivatives utilizing a blue fluorescence emission at 392 nm with a low detection limit (2 nM) and a fast response time (&tild...
Source: Sensors and Actuators B: Chemical - July 13, 2019 Category: Chemistry Source Type: research

The beneficial effect of Indonesian propolis wax from Tetragonula sp. as a therapy in limited vaginal candidiasis patients
This study investigated the effect of Indonesian propolis wax from Tetragonula sp. as a therapy in limited vaginal candidiasis patients. The subjects were women who came to the Tasik Community Health Centre met the inclusion criteria such as clinical complaint and laboratory evaluation (positive hyphae/pseudohyphae and culture on Sabouraud Dextrose Agar (SDA) medium) from a vaginal swab. Evaluation of anti-candida effect of propolis was determined by clinical remission and the absence of Candida’s growth on SDA medium. Forty subjects were randomly assigned to those receiving treatment by ovule propolis (n = 20) a...
Source: Saudi Journal of Biological Sciences - June 29, 2019 Category: Biology Source Type: research

Synthesis and characterization of TPP/chitosan nanoparticles: Colloidal mechanism of reaction and antifungal effect on C. albicans biofilm formation
Publication date: November 2019Source: Materials Science and Engineering: C, Volume 104Author(s): Fabiola Galbiatti de Carvalho, Taís Chaves Magalhães, Natália Moreira Teixeira, Brenna Louise Cavalcanti Gondim, Hugo Lemes Carlo, Rogério Lacerda dos Santos, Alan Reis de Oliveira, Ângelo Marcio Leite DenadaiAbstractIn the present study chitosan (Chit) nanoparticles were synthetized by the ionic gelation process, using tripolyphosphate (TPP) as crosslinking agent. The TPP/Chit nanoparticle formation was evaluated by titrations, measuring electrical conductivity (k), zeta potential (ZP), hydro...
Source: Materials Science and Engineering: C - June 20, 2019 Category: Materials Science Source Type: research

The beneficial effect of Indonesian propolis wax from Tetragonula sp as a therapy in limited vaginal candidiasis patients
This study investigated the effect of Indonesian propolis wax from Tetragonula sp as a therapy in limited vaginal candidiasis patients. The subjects were women who came to the Tasik Community Health Centre met the inclusion criteria such as clinical complaint and laboratory evaluation (positive hyphae/pseudohyphae and culture on Sabouraud Dextrose Agar (SDA) medium) from a vaginal swab. Evaluation of anti-candida effect of propolis was determined by clinical remission and the absence of Candida’s growth on SDA medium. Forty subjects were randomly assigned to those receiving treatment by ovule propolis (n=20) and...
Source: Saudi Journal of Biological Sciences - June 16, 2019 Category: Biology Source Type: research

Degradation of Nystatin in aqueous medium by coupling UV-C irradiation, H2O2 photolysis, and photo-Fenton processes.
Abstract Oxidative degradation and mineralization of the antifungal drug Nystatin (NYS) was investigated using photochemical advanced oxidation processes UV-C irradiation (280-100 nm), H2O2 photolysis (UV/H2O2), and photo-Fenton (UV/H2O2/Fe3+). The effect of operating parameters such as [H2O2], [Fe3+], and [NYS] initial concentrations on degradation efficiency and mineralization ability of different processes was comparatively examined in order to optimize the processes. Photo-Fenton was found to be the most efficient process attaining complete degradation of 0.02 mM (19.2 mg L-1) NYS at 2 ...
Source: Environmental Science and Pollution Research International - June 12, 2019 Category: Environmental Health Authors: Boucenna A, Oturan N, Chabani M, Bouafia-Chergui S, Oturan MA Tags: Environ Sci Pollut Res Int Source Type: research

Antimicrobial assesment of aroylhydrazone derivatives in vitro.
Abstract Aroylhydrazones 1-13 were screened for antimicrobial and antibiofilm activities in vitro. N'-(2-hydroxy-phenylmethylidene)-3-pyridinecarbohydrazide (2), N'-(5-chloro-2-hydroxyphenyl-methylidene)-3-pyridinecarbohydrazide (10), N'-(3,5-chloro-2-hydroxyphenylmethylidene)-3-pyridinecarbohydrazide (11), and N'-(2-hydroxy-5-nitrophenylmethylidene)-3-pyridinecarbohydrazide (12) showed antibacterial activity against Escherichia coli, with MIC values (in µmol mL-1) of 0.18-0.23, 0.11-0.20, 0.16-0.17 and 0.35-0.37, resp. Compounds 11 and 12, as well as N'-(2-hydroxy-3-methoxyphenylmethylidene)-3-pyridinecarbo...
Source: Acta Pharmaceutica - June 1, 2019 Category: Drugs & Pharmacology Authors: Polović S, Bilić VL, Budimir A, Kontrec D, Galić N, Kosalec I Tags: Acta Pharm Source Type: research

The Antifungal Effects of Two Herbal Essences in Comparison with Nystatin on the Candida Strains Isolated from the Edentulous Patients.
CONCLUSION: The anti-Candida effects of fennel and cumin show promise as alternatives to conventional drugs for the treatment of Candida infections. PMID: 31358715 [PubMed - in process] (Source: J Contemp Dent Pract)
Source: J Contemp Dent Pract - June 1, 2019 Category: Dentistry Authors: Khorram Z, Hakimaneh SM, Naeini A, Rafieinezhad R, Salari AM, Shayegh SS Tags: J Contemp Dent Pract Source Type: research

Recreation of in-host acquired single nucleotide polymorphisms by CRISPR-Cas9 reveals an uncharacterised gene playing a role in Aspergillus fumigatus azole resistance via a non-cyp51A mediated resistance mechanism.
Abstract The human host comprises a range of specific niche environments. In order to successfully persist, pathogens such as Aspergillus fumigatus must adapt to these environments. One key example of in-host adaptation is the development of resistance to azole antifungals. Azole resistance in A. fumigatus is increasingly reported worldwide and the most commonly reported mechanisms are cyp51A mediated. Using a unique series of A. fumigatus isolates, obtained from a patient suffering from persistent and recurrent invasive aspergillosis over 2 years, this study aimed to gain insight into the genetic basis of in-host...
Source: Fungal Genetics and Biology - May 22, 2019 Category: Biology Authors: Ballard E, Weber J, Melchers WJG, Tammireddy S, Whitfield PD, Brakhage AA, Brown AJP, Verweij PE, Warris A Tags: Fungal Genet Biol Source Type: research

Erythrocyte ion content and dehydration modulate maximal Gardos channel activity in KCNN4 V282M/+ Hereditary Xerocytosis (HX) red cells.
Abstract Hereditary Xerocytosis (HX) is caused by missense mutations in either themechanosensitive cation channel, PIEZO1 or the Ca2+-activated K+ channel, KCNN4. All HX-associated KCNN4 mutants studied to date revealed increased current magnitude and red cell dehydration. Baseline KCNN4 activity was increased in HX red cells heterozygous for KCNN4mutant V282M. However, HX red cells maximally stimulated by Ca2+ ionophore A23187 or PMCA Ca2+-ATPase inhibitor orthovanadate displayed paradoxically reduced KCNN4 activity. This reduced Ca2+-stimulated mutant KCNN4 activity in HX red cells was associated with unchanged ...
Source: Am J Physiol Cell Ph... - May 15, 2019 Category: Cytology Authors: Rivera A, Vandorpe DH, Shmukler BE, Andolfo I, Iolascon A, Archer NM, Shabani E, Auerbach M, Hamerschlak N, Morton J, Wohlgemuth JG, Brugnara C, Snyder LM, Alper SL Tags: Am J Physiol Cell Physiol Source Type: research

Peritoneal Dialysis-Associated Peritonitis.
Abstract Peritonitis is a common and severe complication in peritoneal dialysis (PD). Detailed recommendations on the prevention and treatment of PD-associated peritonitis have been published by the International Society for Peritoneal Dialysis (ISPD), but there is a substantial variation in clinical practice among dialysis units. Prophylactic antibiotics administered before PD catheter insertion, colonoscopy, or invasive gynecologic procedures, daily topical application of antibiotic cream or ointment to the catheter exit site, and prompt treatment of exit site or catheter infection are key measures to prevent PD...
Source: Clinical Journal of the American Society of Nephrology : CJASN - May 8, 2019 Category: Urology & Nephrology Authors: Szeto CC, Li PK Tags: Clin J Am Soc Nephrol Source Type: research

Candida on oral cavity of pediatric individuals with ALL and its susceptibility to nystatin and amphotericin B
ABSTRACT Objective: The aim of this study was to evaluate the prevalence of Candida colonizationon oral cavity of pediatric individuals with acute lymphocytic leukemia (ALL) and its susceptibility/resistance to nystatin and amphotericin B. Methods: This was a cross sectional study with observational, descriptive and analytic approach. Saliva was collected from40 individuals diagnosed with ALL and from40 healthy subjects, as a comparative group, matched by age and gender with ALL group. The mean age for both groups were 8 years-old. The isolation and identification of the Candidaspecies were performed using the CHROMagarCan...
Source: RGO - Revista Gaucha de Odontologia - May 6, 2019 Category: Dentistry Source Type: research

Incidence and spectrum of yeast species isolated from the oral cavity of Iranian patients suffering from hematological malignancies.
Authors: Arastehfar A, Daneshnia F, Farahyar S, Fang W, Salimi M, Salehi M, Hagen F, Weihua P, Roudbary M, Boekhout T Abstract Background: Oral candidiasis (OC) has a profound effect on the life quality of immunocompromised patients, such as those undergoing chemotherapy. Objective: Systematic investigation of clinical outcome and microbiological features of yeast isolates recovered from the oral cavity of 150 Iranian patients with hematological malignancies. Design: MALDI-TOF MS, 21-plex PCR, and rDNA sequencing were used for identification. Antifungal susceptibility testing (broth mi...
Source: Journal of Oral Microbiology - May 5, 2019 Category: Microbiology Tags: J Oral Microbiol Source Type: research

Phytofabrication of Selenium Nanoparticles From Emblica officinalis Fruit Extract and Exploring Its Biopotential Applications: Antioxidant, Antimicrobial, and Biocompatibility
In conclusion, potential biological applications of PF-SeNPs were explored by antibacterial, antifungal, antioxidant and biocompatibility assays. Materials and Methods Chemicals and Reagents Sodium selenite (98%), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), Tween 80, methanol, acetate buffer, tetracycline, nystatin, rhodamine 123, caspase-3 kit, Dulbecco’s modified Eagle media (DMEM), Folin-Ciocalteu reagent, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), Dulbecco’s phosphate buffered saline pH 7.4 (DPBS) and fetal bovine serum (FBS) w...
Source: Frontiers in Microbiology - April 30, 2019 Category: Microbiology Source Type: research

BET Bromodomain Inhibitors Suppress Inflammatory Activation of Gingival Fibroblasts and Epithelial Cells From Periodontitis Patients
This study was approved by and carried out in accordance with the recommendations of the Bioethical Committee of the Jagiellonian University in Kraków, Poland (permit numbers 122.6120.337.2016 and KBET/310/B/2012). All subjects gave written informed consent in accordance with the Declaration of Helsinki. Clinical characteristics of patients included in the study are shown in Supplementary Table 1. The epithelial layer was separated enzymatically by treatment with dispase at 4°C overnight (o/n) and subjected to three rounds of digestion with trypsin (BioWest) for 10 min at 37°C. After centrifugation, the obt...
Source: Frontiers in Immunology - April 30, 2019 Category: Allergy & Immunology Source Type: research

Staphylococcal and Streptococcal Superantigens Trigger B7/CD28 Costimulatory Receptor Engagement to Hyperinduce Inflammatory Cytokines
Andrey Popugailo, Ziv Rotfogel, Emmanuelle Supper, Dalia Hillman and Raymond Kaempfer* Department of Biochemistry and Molecular Biology, Institute of Medical Research Israel-Canada, The Hebrew University-Hadassah Medical School, Jerusalem, Israel Staphylococcal and streptococcal superantigens are virulence factors that cause toxic shock by hyperinducing inflammatory cytokines. Effective T-cell activation requires interaction between the principal costimulatory receptor CD28 and its two coligands, B7-1 (CD80) and B7-2 (CD86). To elicit an inflammatory cytokine storm, bacterial superantigens must bind directly into t...
Source: Frontiers in Immunology - April 30, 2019 Category: Allergy & Immunology Source Type: research

Effect of Chemically Transformed Macrocyclic Polyene Antibiotics on Tumor Cells
Comparative analysis of the effects of chemically transformed polyene antibiotics pimaricin, nystatin, lucensomycin, amphotericin B, and levorin on biological objectsin vivo andin vitro revealed the greatest biological activity of original amphotericin B and levorin with its derivatives. The study also examined the effects of alkyl derivatives of amphotericin B and levorin modified in certain parts of the lactone ring on the lipid and biological membranes. It is established that methylated levorin possesses larger biological activity than the original antibiotic. Examination of the effects of alkyl derivatives of levorin a...
Source: Bulletin of Experimental Biology and Medicine - April 24, 2019 Category: Biology Source Type: research

Chemical Profiling and Biological Screening of Some River Nile Derived-Microorganisms
Conclusion: The River Nile could be a new source for production of promising bioactive leading compound where antimicrobial and antiparasitic activities may be correlated. Introduction Nature is an exceptional resource of new biologically active compounds with an astonishing chemical diversity such as that found in plant species, animals, and microorganisms (Butler et al., 2014). Microbial diversity constitutes an infinite pool of novel chemistry, making up a valuable source for innovative biotechnology (Bérdy, 2005; Fenical and Jensen, 2006). The aquatic environment is now becoming more appreciated as a ri...
Source: Frontiers in Microbiology - April 12, 2019 Category: Microbiology Source Type: research

Actinobacteria Isolated From Laminaria ochroleuca: A Source of New Bioactive Compounds
Mariana Girão1,2, Inês Ribeiro1, Tiago Ribeiro1, Isabel C. Azevedo1, Filipe Pereira1, Ralph Urbatzka1, Pedro N. Leão1 and Maria F. Carvalho1* 1Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Porto, Portugal 2Department of Biology, Faculty of Sciences, University of Porto, Porto, Portugal Nature is the major reservoir of biologically active molecules. The urgent need of finding novel molecules for pharmaceutical application is prompting the research of underexplored environments, such as marine ecosystems. Here, we investigated cultivable actinobacteria asso...
Source: Frontiers in Microbiology - April 9, 2019 Category: Microbiology Source Type: research

Susceptibility profile of Candida rugosa (Diutina rugosa) against antifungals and compounds of essential oils
Publication date: Available online 5 April 2019Source: Journal de Mycologie MédicaleAuthor(s): A. Ludwig, F.P.K. de Jesus, V. Dutra, S.L. Cândido, S.H. Alves, J.M. SanturioAbstractCandida rugosa (recently reclassified Diutina rugosa) is an emerging pathogen affecting humans and animals. Candida resistance to existing drugs is an important factor to be monitored, as well as the need of researching alternatives to conventional antifungals. Here, we evaluated the in vitro effects of some antifungals and major components of essential oils by the broth microdilution method (CLSI M27-A3) against fifteen C. rugo...
Source: Journal of Medical Mycology - April 7, 2019 Category: Biology Source Type: research

Evaluation of Miracle Mouthwash plus Hydrocortisone Versus Prednisolone Mouth Rinses as Prophylaxis for Everolimus-Associated Stomatitis: A Randomized Phase II Study.
CONCLUSION: Prophylactic use of steroid-containing oral rinses can prevent/ameliorate mIAS in patients with MBC treated with AIE. MMW + hydrocortisone is an affordable option, as is dexamethasone oral rinse. IMPLICATIONS FOR PRACTICE: This prospective phase-II study showed that two steroid-containing mouthrinses substantially reduced incidences of all-grade and grade ≥2 stomatitis and related oral adverse events (AEs), and the number of everolimus dose-delays and/or dose-reduction in metastatic breast cancer (MBC) patients receiving everolimus treatment plus an aromatase inhibitor. Both oral ri...
Source: The Oncologist - March 4, 2019 Category: Cancer & Oncology Authors: Jones VE, McIntyre KJ, Paul D, Wilks ST, Ondreyco SM, Sedlacek S, Melnyk A, Oommen SP, Wang Y, Peck SR, O'Shaughnessy JA Tags: Oncologist Source Type: research

Therapeutic tools for oral candidiasis: Current and new antifungal drugs.
CONCLUSIONS: Nystatin, miconazole, and fluconazole are very effective for treating oral candidiasis. There are systemic alternatives for treating recalcitrant infections, such as the new triazoles, echinocandins, or lipidic presentations of amphotericin B. PMID: 30818309 [PubMed - in process] (Source: Medicina Oral, Patologia Oral y Cirugia Bucal)
Source: Medicina Oral, Patologia Oral y Cirugia Bucal - March 1, 2019 Category: ENT & OMF Tags: Med Oral Patol Oral Cir Bucal Source Type: research

PRISM study: Comparison of a nystatin-neomycin-polymyxin B combination with miconazole for the empirical treatment of infectious vaginitis.
CONCLUSION: The widespread use of NNP for several decades and its good efficacy and safety profile, as well as the frequent diagnostic uncertainties due to the various pathogens sustain the initiation of this broad-spectrum empirical treatment in infectious vaginitis. PMID: 30792037 [PubMed - as supplied by publisher] (Source: Medecine et Maladies Infectieuses)
Source: Medecine et Maladies Infectieuses - February 24, 2019 Category: Infectious Diseases Tags: Med Mal Infect Source Type: research

Pseudonocardia strain improvement for stimulation of the di-sugar heptaene Nystatin-like Pseudonocardia polyene B1 biosynthesis.
Abstract Pseudonocardia autotrophica was previously identified to produce a toxicity-reduced and solubility-improved disaccharide-containing anti-fungal compound belonging to the tetraene-family, Nystatin-like Pseudonocardia Polyene A1 (NPP A1). Subsequently NPP B1, a novel derivative harboring a heptaene core structure, was produced by a pathway-engineered Pseudonocardia strain through inactivation of the specific enoly reductase gene domain in the NPP biosynthetic gene cluster. Although in vitro and in vivo efficacy and toxicity studies indicate that NPP B1 is a promising lead antifungal compound, further improv...
Source: Journal of Industrial Microbiology and Biotechnology - February 23, 2019 Category: Microbiology Authors: Han CY, Jang JY, Kim HJ, Choi S, Kim ES Tags: J Ind Microbiol Biotechnol Source Type: research

Construction of a Pseudozyma antarctica strain without foreign DNA sequences (self-cloning strain) for high yield production of a biodegradable plastic-degrading enzyme.
Abstract The basidiomycetous yeast Pseudozyma antarctica GB-4(0) esterase (PaE) is a promising candidate for accelerating degradation of used biodegradable plastics (BPs). To increase safety and reduce costs associated with the use of PaE, we constructed a self-cloning strain with high-PaE productivity. A Lys12 gene (PaLYS12)-deleted lysine auxotroph strain GB4-(0)-L1 was obtained from GB-4(0) by ultraviolet mutagenesis and nystatin enrichment. Subsequently, the PaE gene (PaCLE1) expression cassette consisting of GB-4(0)-derived PaCLE1, under the control of a xylose-inducible xylanase promoter with PaLYS12, was ra...
Source: Bioscience, Biotechnology, and Biochemistry - February 3, 2019 Category: Biochemistry Authors: Sameshima-Yamashita Y, Watanabe T, Tanaka T, Tsuboi S, Yarimizu T, Morita T, Koike H, Suzuki K, Kitamoto H Tags: Biosci Biotechnol Biochem Source Type: research

Chemical characterization and antioxidant, cytotoxic, antibacterial, and antifungal properties of ethanolic extract of Allium Saralicum R.M. Fritsch leaves rich in linolenic acid, methyl ester
In conclusion, the findings showed the useful antioxidant, non-cytotoxic, antibacterial, and antifungal effects of A. Saralicum ethanolic extract.Graphical Abstract (Source: Journal of Photochemistry and Photobiology B: Biology)
Source: Journal of Photochemistry and Photobiology B: Biology - January 30, 2019 Category: Speech-Language Pathology Source Type: research

Hydroxyl alkyl ammonium ionic liquid assisted green and one-pot regioselective access to functionalized pyrazolodihydropyridine core and their pharmacological evaluation.
el HM Abstract Herein our team explored a promising synthetic trail to Functionalized pyrazolodihydropyridine core using hydroxyl alkyl ammonium ionic liquid via one-pot fusion of 3-methyl-1-phenyl-1H-pyrazole-5-amine, different heterocyclic aldehydes and 1, 3-Cyclic diones. The aimed compounds were obtained by Domino-Knoevenagel condensation and Michael addition followed by cyclization. The reaction transformation involves the formation of two CC and one CN bond formation. The perspective of the present work is selectively approached to Functionalized pyrazolodihydropyridine core excluding other potential paralle...
Source: Bioorganic Chemistry - January 19, 2019 Category: Chemistry Authors: Patel DM, Sharma MG, Vala RM, Lagunes I, Puerta A, Padrón JM, Rajani DP, Patel HM Tags: Bioorg Chem Source Type: research

The Effectiveness of Nigella Sativa Alcoholic Extract on the Inhibition of Candida Albicans Colonization and Formation of Plaque on Acrylic Denture Plates: an In Vitro Study
In this study, 30 pieces of acrylic resin were contaminated bycandida albicanssuspension. Then, the acrylic pieces were randomly divided into six groups and treated with 0.2, 0.4, 20, and 200 mg/ml ofNigella sativa, 100,000 units of nystatin (positive control), and distilled water (negative control) for 8 hours. At the end of the exposure period of the drugs, the rinse solution from acrylic pieces was cultured in Sabouraud Dextrose Agar and the average of the colonies from each group was compared.  Results: The average number of colonies obtained at concentrations of 0.2, 0.4, 20, and 200 mg/ml ofNigella sativawere 12...
Source: Journal of Dentistry, Shiraz University of Medical Sciences - January 12, 2019 Category: Dentistry Source Type: research