Breeding of Saccharomyces cerevisiae with a High-Throughput Screening Strategy for Improvement of S-Adenosyl-L-Methionine Production
Appl Biochem Biotechnol. 2023 Jul 7. doi: 10.1007/s12010-023-04622-7. Online ahead of print.ABSTRACTS-adenosyl-l-methionine (SAM), a vital physiologically active substance in living organisms, is produced by fermentation over Saccharomyces cerevisiae. The main limitation in SAM production was the low biosynthesis ability of SAM in S. cerevisiae. The aim of this work is to breed an SAM-overproducing mutant through UV mutagenesis coupled with high-throughput selection. Firstly, a high-throughput screening method by rapid identification of positive colonies was conducted. White colonies on YND medium were selected as positive...
Source: Applied Biochemistry and Biotechnology - July 7, 2023 Category: Biochemistry Authors: Zhong-Ce Hu Yun-Chao Tao Jun-Chao Pan Chui-Mu Zheng Yuan-Shan Wang Ya-Ping Xue Zhi-Qiang Liu Yu-Guo Zheng Source Type: research
Breeding of Saccharomyces cerevisiae with a High-Throughput Screening Strategy for Improvement of S-Adenosyl-L-Methionine Production
Appl Biochem Biotechnol. 2023 Jul 7. doi: 10.1007/s12010-023-04622-7. Online ahead of print.ABSTRACTS-adenosyl-l-methionine (SAM), a vital physiologically active substance in living organisms, is produced by fermentation over Saccharomyces cerevisiae. The main limitation in SAM production was the low biosynthesis ability of SAM in S. cerevisiae. The aim of this work is to breed an SAM-overproducing mutant through UV mutagenesis coupled with high-throughput selection. Firstly, a high-throughput screening method by rapid identification of positive colonies was conducted. White colonies on YND medium were selected as positive...
Source: Applied Biochemistry and Biotechnology - July 7, 2023 Category: Biochemistry Authors: Zhong-Ce Hu Yun-Chao Tao Jun-Chao Pan Chui-Mu Zheng Yuan-Shan Wang Ya-Ping Xue Zhi-Qiang Liu Yu-Guo Zheng Source Type: research
Breeding of Saccharomyces cerevisiae with a High-Throughput Screening Strategy for Improvement of S-Adenosyl-L-Methionine Production
Appl Biochem Biotechnol. 2023 Jul 7. doi: 10.1007/s12010-023-04622-7. Online ahead of print.ABSTRACTS-adenosyl-l-methionine (SAM), a vital physiologically active substance in living organisms, is produced by fermentation over Saccharomyces cerevisiae. The main limitation in SAM production was the low biosynthesis ability of SAM in S. cerevisiae. The aim of this work is to breed an SAM-overproducing mutant through UV mutagenesis coupled with high-throughput selection. Firstly, a high-throughput screening method by rapid identification of positive colonies was conducted. White colonies on YND medium were selected as positive...
Source: Applied Biochemistry and Biotechnology - July 7, 2023 Category: Biochemistry Authors: Zhong-Ce Hu Yun-Chao Tao Jun-Chao Pan Chui-Mu Zheng Yuan-Shan Wang Ya-Ping Xue Zhi-Qiang Liu Yu-Guo Zheng Source Type: research
TPE-based fluorescent probe for dual channel imaging of pH/viscosity and selective visualization of cancer cells and tissues
Talanta. 2023 Jun 22;265:124862. doi: 10.1016/j.talanta.2023.124862. Online ahead of print.ABSTRACTThe development of efficient fluorescence-based detection tools with high contrast and accuracy in cancer diagnosis has recently attracted extensive attention. Changes in the microenvironments between cancer and normal cells provide new biomarkers for precise and comprehensive cancer diagnosis. Herein, a dual-organelle-targeted probe with multiple-parameter response is developed to realize cancer detection. We designed a tetraphenylethylene (TPE)-based fluorescent probe TPE-PH-KD connected with quinolinium group for simultane...
Source: Talanta - June 28, 2023 Category: Chemistry Authors: Linlin Yang Pengli Gu Aoxiang Fu Yanbei Xi Shaoli Cui Liguo Ji Lili Li Nana Ma Qingzhi Wang Guangjie He Source Type: research
Reviving the interest in the versatile drug nystatin: a multitude of strategies to increase its potential as an effective and safe antifungal agent
Adv Drug Deliv Rev. 2023 Jun 20:114969. doi: 10.1016/j.addr.2023.114969. Online ahead of print.ABSTRACTNystatin is an antifungal molecule with a remarkable yet squandered versatility. In this review, its mechanism of action is explored, along with its extensive action spectrum and toxicity. A multitude of methodologies to tackle the drug's physical and chemical hurdles are outlined along with some proven-effective strategies to increase its activity and/or decrease its toxicity. A separate detailed section focused on micro and nanotechnology solutions addresses new drug delivery systems made of polymeric, metallic or lipid...
Source: Advanced Drug Delivery Reviews - June 22, 2023 Category: Drugs & Pharmacology Authors: Filipa Sousa Cec ília Nascimento Domingos Ferreira Salette Reis Paulo Costa Source Type: research
Reviving the interest in the versatile drug nystatin: a multitude of strategies to increase its potential as an effective and safe antifungal agent
Adv Drug Deliv Rev. 2023 Jun 20:114969. doi: 10.1016/j.addr.2023.114969. Online ahead of print.ABSTRACTNystatin is an antifungal molecule with a remarkable yet squandered versatility. In this review, its mechanism of action is explored, along with its extensive action spectrum and toxicity. A multitude of methodologies to tackle the drug's physical and chemical hurdles are outlined along with some proven-effective strategies to increase its activity and/or decrease its toxicity. A separate detailed section focused on micro and nanotechnology solutions addresses new drug delivery systems made of polymeric, metallic or lipid...
Source: Advanced Drug Delivery Reviews - June 22, 2023 Category: Drugs & Pharmacology Authors: Filipa Sousa Cec ília Nascimento Domingos Ferreira Salette Reis Paulo Costa Source Type: research
Oral manifestations of psoriasis
A 67-year-old male patient complained of pain and burning in the oral mucosa. On physical examination, erythematous plaques with whitish areas on the bilateral buccal mucosa and angular cheilitis were observed. Well-demarcated erythematous plaques covered by keratotic scales were also observed in the region of the ankle, trunk, and abdomen. Topical use of Nystatin and Miconazole was prescribed, but with no remission of the lesions and symptoms. Patient reported having Psoriasis and Psoriatic Arthritis. (Source: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics)
Source: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics - June 15, 2023 Category: ENT & OMF Authors: Sic ília Rezende OLIVEIRA, Ana Carolina Velasco Pondé de SENA, Thalita Soares TAVARES, Fernanda Aragão FELIX, Denise Vieira TRAVASSOS, Tarcília Aparecida da SILVA Source Type: research
Molecules, Vol. 28, Pages 4777: Synthesis, Characterization, DFT Studies of Novel Cu(II), Zn(II), VO(II), Cr(III), and La(III) Chloro-Substituted Schiff Base Complexes: Aspects of Its Antimicrobial, Antioxidant, Anti-Inflammatory, and Photodegradation of Methylene Blue
a Abou El-ezz
A new chlorobenzylidene imine ligand, (E)-1-((5-chloro-2-hydroxybenzylidene)amino) naphthalen-2-ol (HL), and its [Zn(L)(NO3)(H2O)3], [La(L)(NO3)2(H2O)2], [VO(L)(OC2H5)(H2O)2], [Cu(L)(NO3)(H2O)3], and [Cr(L)(NO3)2(H2O)2], complexes were synthesized and characterized. The characterization involved elemental analysis, FT-IR, UV/Vis, NMR, mass spectra, molar conductance, and magnetic susceptibility measurements. The obtained data confirmed the octahedral geometrical structures of all metal complexes, while the [VO(L)(OC2H5)(H2O)2] complex exhibited a distorted square pyramidal structure. The complexes were fo...
Source: Molecules - June 15, 2023 Category: Chemistry Authors: Laila H. Abdel-Rahman Maram T. Basha Badriah Saad Al-Farhan Walaa Alharbi Mohamed R. Shehata Noura O. Al Zamil Doaa Abou El-ezz Tags: Article Source Type: research
Molecules, Vol. 28, Pages 4304: Chenopodium murale Juice Shows Anti-Fungal Efficacy in Experimental Oral Candidiasis in Immunosuppressed Rats in Relation to Its Chemical Profile
M. ELsayed
Chenopodium murale (Syn. Chenopodiastrum murale) (amaranthaceae) is used in the rural Egypt to treat oral ulcers in newborn children. The current study aimed to discover new natural products suitable for treating candidiasis disease with minimal side effects. Characterization of bioactive compounds by LC-QTOF-HR-MS/MS from Chenopodium murale fresh leaves’ juice (CMJ) was carried out in order to elucidate their potential anti-fungal and immunomodulatory effects in oral candidiasis in immunosuppressed rats. An oral ulcer candidiasis model was created in three stages: (i) immunosuppression by dri...
Source: Molecules - May 24, 2023 Category: Chemistry Authors: Samah A. El-Newary Asmaa S. Abd Elkarim Nayera A. M. Abdelwahed Elsayed A. Omer Abdelbaset M. Elgamal Wael M. ELsayed Tags: Article Source Type: research
Isolation and Identification of Flavanone Derivative Eriodictyol from the Methanol Extract of < em > Afzelia africana < /em > Bark and Its Antimicrobial and Antioxidant Activities
CONCLUSION: The study reports for the first time the identification of eriodictyol from the bark of A. africana which exhibited significant antioxidant and antimicrobial properties.PMID:37200890 | PMC:PMC10188263 | DOI:10.1155/2023/9345047 (Source: Evidence-based Complementary and Alternative Medicine)
Source: Evidence-based Complementary and Alternative Medicine - May 18, 2023 Category: Complementary Medicine Authors: Bright Yaw Vigbedor Clement Osei Akoto David Neglo Source Type: research
