Chlordiazepoxide against signalling, receptor and regulatory proteins of breast cancer: a structure-based in-silico approach
In this study, we included four crucial proteins that perform signalling, receptor, and regulatory action, namely- NUDIX Hydrolases, Dihydrofolate Reductase, HER2/neu Kinase and EGFR and performed multitargeted molecular docking studies against human-approved drugs using HTVS, SP and extra precise algorithms and filtered the poses with MM\GBSA, suggested a benzodiazepine derivative chlordiazepoxide, used as an anxiolytic agent, can be a multitargeted inhibitor with docking and MM\GBSA score ranging from - 4.628 to - 7.877 and - 18.59 to - 135.86 kcal/mol, respectively, and the most interacted residues were 6ARG, 6GLU, 3TRP...
Source: Molecular Medicine - April 17, 2024 Category: Molecular Biology Authors: Ahad Amer Alsaiari Amal F Gharib Maha Mahfouz Bakhuraysah Amani A Alrehaili Shatha M Algethami Hayfa Ali Alsaif Norah Al Harthi Mohammed Ageeli Hakami Source Type: research
Lipase and Protease Production Ability of Multi-drug Resistant Bacteria Worsens the Outcomes of Wound Infections
CONCLUSION: In conclusion, the findings suggest that lipases and proteases produced by bacteria could contribute to drug resistance and act as virulence factors in the development of surgical site infections. Understanding the role of these enzymes may inform strategies for preventing and managing post-surgical wound infections more effectively.PMID:38629357 | DOI:10.2174/0113816128302189240402043330 (Source: Current Pharmaceutical Design)
Source: Current Pharmaceutical Design - April 17, 2024 Category: Drugs & Pharmacology Authors: Attaur Rahman Saiqa Sardar Zeeshan Niaz Asif Khan None Sheheryar Abdulwahed Fahad Alrefaei Muhammad Hamayun Sajid Ali Source Type: research
Molecules, Vol. 29, Pages 1820: Novel and Sensitive Touchdown Polymerase Chain Reaction Assays for the Detection of Goat and Sheep Milk Adulteration with Cow Milk
In this study, a specific qualitative touchdown (TD) PCR method was developed to detect the undeclared addition of cow’s milk in goat and sheep milk based on the discrimination of the peak areas of the melting curves after the modification of bovine-specific primers. The developed methodology has high specificity for the DNA templates of other species, such as buffalos and donkeys, and is able to identify the presence of cow’s milk down to 1%. Repeatability was tested at low bovine concentrations of 5% and 1% and resulted in %RSD values of 1.53–2.04 for the goat–cow a...
Source: Molecules - April 17, 2024 Category: Chemistry Authors: Ariadni Kourkouli Nikolaos Thomaidis Marilena Dasenaki Athina Markou Tags: Article Source Type: research
Factors Associated with Low Volumes of Mother ’s Own Milk at Neonatal Intensive Care Unit Discharge of Very Low Birth Weight Infants—a Cohort Study
Breastfeeding Medicine, Ahead of Print. (Source: Breastfeeding Medicine)
Source: Breastfeeding Medicine - April 17, 2024 Category: Perinatology & Neonatology Authors: Mariana Gonz ález de Oliveira Desir ée de Freitas Valle Volkmer Arthur Pille Jonas Wolf Mar ôla Flores da Cunha Scheeren Source Type: research
Chlordiazepoxide against signalling, receptor and regulatory proteins of breast cancer: a structure-based in-silico approach
In this study, we included four crucial proteins that perform signalling, receptor, and regulatory action, namely- NUDIX Hydrolases, Dihydrofolate Reductase, HER2/neu Kinase and EGFR and performed multitargeted molecular docking studies against human-approved drugs using HTVS, SP and extra precise algorithms and filtered the poses with MM\GBSA, suggested a benzodiazepine derivative chlordiazepoxide, used as an anxiolytic agent, can be a multitargeted inhibitor with docking and MM\GBSA score ranging from − 4.628 to − 7.877 and − 18.59 to − 135.86 kcal/mol, respectively, and the most interacted residues were 6ARG, 6G...
Source: Medical Oncology - April 17, 2024 Category: Cancer & Oncology Source Type: research
Sialic acid-based probiotic intervention in lactating mothers improves the neonatal gut microbiota and immune responses by regulating sialylated milk oligosaccharide synthesis via the gut –breast axis
Volume 16, Issue 1, January-December 2024 . (Source: Gut Microbes)
Source: Gut Microbes - April 17, 2024 Category: Microbiology Authors: Yushuang WangBinqi RuiXiaolei ZeYujia LiuDa YuYinhui LiuZhi LiYu XiXixi NingZengjie LeiJieli YuanLiang LiXuguang ZhangWenzhe LiYanjie DengJingyu YanMing Lia Department of Microecology, College of Basic Medical Science, Dalian Medical University, Dalian, C Source Type: research
Probiotic icecream as a functional food - a review
S. Sarkar Nutrition & Food Science, Vol. ahead-of-print, No. ahead-of-print, pp.- Globally, consumer’s inclination towards functional foods had noticed due to their greater health consciousness coupled with enhanced health-care cost. The fact that probiotics could promote a healthier gut microbiome led projection of probiotic foods as functional foods and had emerged as an important dietary strategy for improved human health. It had established that ice cream was a better carrier for probiotics than fermented milked due to greater stability of probiotics in ice cream matrix. Global demand for ice cream boomed and pr...
Source: Nutrition and Food Science - April 16, 2024 Category: Nutrition Authors: S. Sarkar Source Type: research
MiR-2284b regulation of α-s1 casein synthesis in mammary epithelial cells of dairy goats
Anim Biotechnol. 2024 Nov;35(1):2334725. doi: 10.1080/10495398.2024.2334725. Epub 2024 Apr 16.ABSTRACTThe lactation character of dairy goats is the most important characteristic, and milk protein is an important index to evaluate milk quality. Casein accounts for more than 80% of the total milk protein in goat milk and is the main component of milk protein. Using GMECs (goat mammary epithelial cells) as the research object, the CHECK2 vector of the CSN1S1 gene and the overexpression vector of pcDNA 3.1 were constructed, and the mimics of miR-2284b and the interfering RNA of CSN1S1 were synthesized. Using PCR, RT-qPCR, a du...
Source: Animal Biotechnology - April 16, 2024 Category: Biotechnology Authors: Jinxing Hou Wenfei Li Xiaolong Xu Ao Sun Ganggang Xu Zefang Cheng Haoyuan Zhang Xiaopeng An Source Type: research
MiR-2284b regulation of α-s1 casein synthesis in mammary epithelial cells of dairy goats
Anim Biotechnol. 2024 Nov;35(1):2334725. doi: 10.1080/10495398.2024.2334725. Epub 2024 Apr 16.ABSTRACTThe lactation character of dairy goats is the most important characteristic, and milk protein is an important index to evaluate milk quality. Casein accounts for more than 80% of the total milk protein in goat milk and is the main component of milk protein. Using GMECs (goat mammary epithelial cells) as the research object, the CHECK2 vector of the CSN1S1 gene and the overexpression vector of pcDNA 3.1 were constructed, and the mimics of miR-2284b and the interfering RNA of CSN1S1 were synthesized. Using PCR, RT-qPCR, a du...
Source: Animal Biotechnology - April 16, 2024 Category: Biotechnology Authors: Jinxing Hou Wenfei Li Xiaolong Xu Ao Sun Ganggang Xu Zefang Cheng Haoyuan Zhang Xiaopeng An Source Type: research
Occurrence, antimicrobial susceptibility, and resistance genes of Staphylococcus aureus in milk and milk products in the Arsi highlands of Ethiopia
In Ethiopia, milk production and handling practices often lack proper hygiene measures, leading to the potential contamination of milk and milk products with Staphylococcus aureus (S. aureus), including methicill... (Source: BMC Microbiology)
Source: BMC Microbiology - April 16, 2024 Category: Microbiology Authors: Abiot Deddefo, Gezahegne Mamo, Minda Asfaw, Adem Edao, Adem Hiko, Dereje Fufa, Mohammed Jafer, Melaku Sombo and Kebede Amenu Tags: Research Source Type: research
Oral iron supplementation: new formulations, old questions
Haematologica. 2024 Apr 11. doi: 10.3324/haematol.2024.284967. Online ahead of print.ABSTRACTIron deficiency anemia and pre-anemic iron deficiency are the most frequent pathologies. The first line of treatment involves oral iron supplementation. The simplest, least expensive, and most frequently prescribed drug is ferrous sulfate, while other ferrous salts and ferric complexes with polysaccharides or succinylated milk proteins are also widely used. In recent years, novel iron formulations have been developed, such as the lipophilic iron donor ferric maltol, or nanoparticle encapsulated sucrosomial® iron. Oral iron supplem...
Source: Haematologica - April 15, 2024 Category: Hematology Authors: Kostas Pantopoulos Source Type: research
Necrotizing enterocolitis in a premature infant
We report a case of necrotizing enterocolitis in a 30 weeks old premature infant fed with breast milk. No ischemic etiology was present. Bacteriological and parasitological investigations were found to be negative. Only, a positive latex agglutination test for Adenoviruses was detected in stools collected the day of the bloody diarrhea. Detailed microbiological examinations are emphasized by this unusual finding because the role of pathogens in this disease deserves further understanding.PMID:38620191 | PMC:PMC7141009 | DOI:10.1016/S0399-077X(05)81185-0 (Source: Medecine et Maladies Infectieuses)
Source: Medecine et Maladies Infectieuses - April 15, 2024 Category: Infectious Diseases Authors: R Mettey G Agius C Cessans Source Type: research
Enterovirus Infection in Preterm Quadruplets with Clinical Presentation of Neonatal Late-Onset Sepsis: a Case Report
Z Geburtshilfe Neonatol. 2024 Apr 15. doi: 10.1055/a-2279-6803. Online ahead of print.ABSTRACTThis is the unusual case of concurrent late-onset sepsis caused by enteroviruses in preterm quadruplets in a neonatal ward. While viral infections are an important differential diagnosis of bacterial sepsis in the outpatient setting, the occurrence in a neonatal ward with strict prophylactic hygiene measures is unusual. In this case, transmission of enteroviruses via breast milk appears possible.PMID:38621739 | DOI:10.1055/a-2279-6803 (Source: Zeitschrift fur Geburtshilfe und Neonatologie)
Source: Zeitschrift fur Geburtshilfe und Neonatologie - April 15, 2024 Category: Perinatology & Neonatology Authors: Viktor Seifert Lena B öck Johannes Wirbelauer Source Type: research
Isolation and characterization of novel Staphylococcus aureus bacteriophage Hesat from dairy origin
Appl Microbiol Biotechnol. 2024 Apr 15;108(1):299. doi: 10.1007/s00253-024-13129-y.ABSTRACTA novel temperate phage, named Hesat, was isolated by the incubation of a dairy strain of Staphylococcus aureus belonging to spa-type t127 with either bovine or ovine milk. Hesat represents a new species of temperate phage within the Phietavirus genus of the Azeredovirinae subfamily. Its genome has a length of 43,129 bp and a GC content of 35.11% and contains 75 predicted ORFs, some of which linked to virulence. This includes (i) a pathogenicity island (SaPln2), homologous to the type II toxin-antitoxin system PemK/MazF family toxin;...
Source: Applied Microbiology and Biotechnology - April 15, 2024 Category: Microbiology Authors: Barbara Turchi Claudia Campobasso Arianna Nardinocchi Jeroen Wagemans Beatrice Torracca C édric Lood Graziano Di Giuseppe Paola Nieri Fabrizio Bertelloni Luca Turini Valeria Ruffo Rob Lavigne Mariagrazia Di Luca Source Type: research
