Analysis of spike and accessory 3c genes mutations of less virulent and FIP-associated feline coronaviruses in Beijing, China
Virology. 2023 Oct 30;589:109919. doi: 10.1016/j.virol.2023.109919. Online ahead of print.ABSTRACTMutations in S and 3c genes of feline coronavirus (FCoV) have been associated with the development of feline infectious peritonitis (FIP). In the present study, FCoV S and 3c genes mutations were analyzed in healthy and FIP cats. M1058L mutation was found in 13.64% (3/22) feces from FIP cats, but not in feces from healthy cats (0/39). The intact 3c gene was found in feces from both healthy cats (19/19) and FIP cats (12/12). All parenteral samples from FIP cats carried one or more of the M1058L mutation, S1060A mutation and mut...
Source: Virology - November 8, 2023 Category: Virology Authors: Jingru Zhu Shuqi Deng Danxia Mou Gege Zhang Yingying Fu Wei Huang Yueping Zhang Yanli Lyu Source Type: research
Murine norovirus infection of macrophages induces intrinsic apoptosis as the major form of programmed cell death
Virology. 2023 Oct 31;589:109921. doi: 10.1016/j.virol.2023.109921. Online ahead of print.ABSTRACTHuman norovirus is the leading cause of acute gastroenteritis worldwide, however despite the significance of this pathogen, we have a limited understanding of how noroviruses cause disease, and modulate the innate immune response. Programmed cell death (PCD) is an important part of the innate response to invading pathogens, but little is known about how specific PCD pathways contribute to norovirus replication. Here, we reveal that murine norovirus (MNV) virus-induced PCD in macrophages correlates with the release of infectiou...
Source: Virology - November 8, 2023 Category: Virology Authors: Joshua M Deerain Turgut E Aktepe Alice M Trenerry Gregor Ebert Jennifer L Hyde Katelyn Charry Laura Edgington-Mitchell Banyan Xu Rebecca L Ambrose Soroush T Sarvestani Kate E Lawlor Jaclyn S Pearson Peter A White Jason M Mackenzie Source Type: research
Analysis of spike and accessory 3c genes mutations of less virulent and FIP-associated feline coronaviruses in Beijing, China
Virology. 2023 Oct 30;589:109919. doi: 10.1016/j.virol.2023.109919. Online ahead of print.ABSTRACTMutations in S and 3c genes of feline coronavirus (FCoV) have been associated with the development of feline infectious peritonitis (FIP). In the present study, FCoV S and 3c genes mutations were analyzed in healthy and FIP cats. M1058L mutation was found in 13.64% (3/22) feces from FIP cats, but not in feces from healthy cats (0/39). The intact 3c gene was found in feces from both healthy cats (19/19) and FIP cats (12/12). All parenteral samples from FIP cats carried one or more of the M1058L mutation, S1060A mutation and mut...
Source: Virology - November 8, 2023 Category: Virology Authors: Jingru Zhu Shuqi Deng Danxia Mou Gege Zhang Yingying Fu Wei Huang Yueping Zhang Yanli Lyu Source Type: research
Broad spectrum post-entry inhibitors of coronavirus replication: Cardiotonic steroids and monensin
Virology. 2023 Oct 31;589:109915. doi: 10.1016/j.virol.2023.109915. Online ahead of print.ABSTRACTA small molecule screen identified several cardiotonic steroids (digitoxin and ouabain) and the ionophore monensin as potent inhibitors of HCoV-229E, HCoV-OC43, and SARS-CoV-2 replication with EC50s in the low nM range. Subsequent tests confirmed antiviral activity in primary cell models including human nasal epithelial cells and lung organoids. Addition of digitoxin, ouabain, or monensin strongly reduced viral gene expression as measured by both viral protein and RNA accumulation. Furthermore, the compounds acted post virus e...
Source: Virology - November 6, 2023 Category: Virology Authors: Shahrzad Jahanshahi Hong Ouyang Choudhary Ahmed Ali Zahedi Amiri Subha Dahal Yu-Qian Mao David A J Van Ommen Ramy Malty Wenming Duan Terek Been Javier Hernandez Maria Mangos Jocelyn Nurtanto Mohan Babu Liliana Attisano Walid A Houry Theo J Moraes Alan Coc Source Type: research
Pseudotyped zoonotic thogotoviruses exhibit broad entry range in mammalian cells
This study therefore developed a cell-cell fusion assay and three pseudotyping tools and used them to assess envelope function and cell tropism. Envelope glycoproteins of Dhori (DHOV), Thogoto (THOV), Bourbon, and Sinu viruses were all revealed to exhibit pH-dependent triggering of membrane fusion. Lentivirus vectors were robustly pseudotyped with these glycoproteins while influenza virus vectors showed pseudotyping compatibility, albeit at lower efficiencies. Replication-competent vesicular stomatitis virus expressing DHOV or THOV glycoproteins were also successfully generated. These pseudotyped viruses mediated entry int...
Source: Virology - November 6, 2023 Category: Virology Authors: Thiprampai Thamamongood Juggragarn Jengarn Papon Muangsanit Nalinrat Petpiroon Kanjana Srisutthisamphan Khemphitcha Attasombat Ratjika Wongwanakul Sasitorn Aueviriyavit Sira Laohathai Anan Jongkaewwattana Samaporn Teeravechyan Source Type: research
Broad spectrum post-entry inhibitors of coronavirus replication: Cardiotonic steroids and monensin
Virology. 2023 Oct 31;589:109915. doi: 10.1016/j.virol.2023.109915. Online ahead of print.ABSTRACTA small molecule screen identified several cardiotonic steroids (digitoxin and ouabain) and the ionophore monensin as potent inhibitors of HCoV-229E, HCoV-OC43, and SARS-CoV-2 replication with EC50s in the low nM range. Subsequent tests confirmed antiviral activity in primary cell models including human nasal epithelial cells and lung organoids. Addition of digitoxin, ouabain, or monensin strongly reduced viral gene expression as measured by both viral protein and RNA accumulation. Furthermore, the compounds acted post virus e...
Source: Virology - November 6, 2023 Category: Virology Authors: Shahrzad Jahanshahi Hong Ouyang Choudhary Ahmed Ali Zahedi Amiri Subha Dahal Yu-Qian Mao David A J Van Ommen Ramy Malty Wenming Duan Terek Been Javier Hernandez Maria Mangos Jocelyn Nurtanto Mohan Babu Liliana Attisano Walid A Houry Theo J Moraes Alan Coc Source Type: research
Pseudotyped zoonotic thogotoviruses exhibit broad entry range in mammalian cells
This study therefore developed a cell-cell fusion assay and three pseudotyping tools and used them to assess envelope function and cell tropism. Envelope glycoproteins of Dhori (DHOV), Thogoto (THOV), Bourbon, and Sinu viruses were all revealed to exhibit pH-dependent triggering of membrane fusion. Lentivirus vectors were robustly pseudotyped with these glycoproteins while influenza virus vectors showed pseudotyping compatibility, albeit at lower efficiencies. Replication-competent vesicular stomatitis virus expressing DHOV or THOV glycoproteins were also successfully generated. These pseudotyped viruses mediated entry int...
Source: Virology - November 6, 2023 Category: Virology Authors: Thiprampai Thamamongood Juggragarn Jengarn Papon Muangsanit Nalinrat Petpiroon Kanjana Srisutthisamphan Khemphitcha Attasombat Ratjika Wongwanakul Sasitorn Aueviriyavit Sira Laohathai Anan Jongkaewwattana Samaporn Teeravechyan Source Type: research
Broad spectrum post-entry inhibitors of coronavirus replication: Cardiotonic steroids and monensin
Virology. 2023 Oct 31;589:109915. doi: 10.1016/j.virol.2023.109915. Online ahead of print.ABSTRACTA small molecule screen identified several cardiotonic steroids (digitoxin and ouabain) and the ionophore monensin as potent inhibitors of HCoV-229E, HCoV-OC43, and SARS-CoV-2 replication with EC50s in the low nM range. Subsequent tests confirmed antiviral activity in primary cell models including human nasal epithelial cells and lung organoids. Addition of digitoxin, ouabain, or monensin strongly reduced viral gene expression as measured by both viral protein and RNA accumulation. Furthermore, the compounds acted post virus e...
Source: Virology - November 6, 2023 Category: Virology Authors: Shahrzad Jahanshahi Hong Ouyang Choudhary Ahmed Ali Zahedi Amiri Subha Dahal Yu-Qian Mao David A J Van Ommen Ramy Malty Wenming Duan Terek Been Javier Hernandez Maria Mangos Jocelyn Nurtanto Mohan Babu Liliana Attisano Walid A Houry Theo J Moraes Alan Coc Source Type: research
Pseudotyped zoonotic thogotoviruses exhibit broad entry range in mammalian cells
This study therefore developed a cell-cell fusion assay and three pseudotyping tools and used them to assess envelope function and cell tropism. Envelope glycoproteins of Dhori (DHOV), Thogoto (THOV), Bourbon, and Sinu viruses were all revealed to exhibit pH-dependent triggering of membrane fusion. Lentivirus vectors were robustly pseudotyped with these glycoproteins while influenza virus vectors showed pseudotyping compatibility, albeit at lower efficiencies. Replication-competent vesicular stomatitis virus expressing DHOV or THOV glycoproteins were also successfully generated. These pseudotyped viruses mediated entry int...
Source: Virology - November 6, 2023 Category: Virology Authors: Thiprampai Thamamongood Juggragarn Jengarn Papon Muangsanit Nalinrat Petpiroon Kanjana Srisutthisamphan Khemphitcha Attasombat Ratjika Wongwanakul Sasitorn Aueviriyavit Sira Laohathai Anan Jongkaewwattana Samaporn Teeravechyan Source Type: research
Selection, activity, and nuclease stabilization of cross-neutralizing DNA aptamers targeting HSV-1 and HSV-2
Virology. 2023 Oct 27;589:109916. doi: 10.1016/j.virol.2023.109916. Online ahead of print.ABSTRACTCross-neutralizing aptamers targeting both HSV-1 and HSV-2 were developed by selecting against the ectodomains of glycoprotein D (gD) from both viruses in parallel as well as sequentially using the SELEX method. Since gD facilitates viral invasion, sterically blocking the host-receptor interaction prevents infection. Candidate aptamers were screened, and lead aptamers were identified that exhibited exceptional neutralizing activity against both viruses in vitro. The specificity of the aptamers was confirmed by comparing their ...
Source: Virology - November 5, 2023 Category: Virology Authors: Michael D Schump David I Bernstein Fernando J Bravo Charles P Neff Source Type: research
Considerations in engineering viral vectors for genome editing in plants
Virology. 2023 Oct 30;589:109922. doi: 10.1016/j.virol.2023.109922. Online ahead of print.ABSTRACTPlant viruses have been engineered to express proteins and induce gene silencing for decades. Recently, plant viruses have also been used to deliver components into plant cells for genome editing, a technique called virus-induced genome editing (VIGE). Although more than a dozen plant viruses have been engineered into VIGE vectors and VIGE has been successfully accomplished in some plant species, application of VIGE to crops that are difficult to tissue culture and/or have low regeneration efficiency is still tough. This paper...
Source: Virology - November 4, 2023 Category: Virology Authors: Xiaoyun Wu Ying Zhang Xue Jiang Tingshuai Ma Yating Guo Xiaoxia Wu Yushuang Guo Xiaofei Cheng Source Type: research
Detection and diversity of gastrointestinal viruses in wastewater from Caracas, Venezuela, 2021-2022
Virology. 2023 Oct 23;589:109913. doi: 10.1016/j.virol.2023.109913. Online ahead of print.ABSTRACTGastrointestinal viruses (GIV) are an important cause of childhood morbidity and mortality, particularly in developing countries. Their epidemiological impact in Venezuela during the COVID-19 pandemic remains unclear. GIV can also be detected in domestic sewage. Ninety-one wastewater samples from urban areas of Caracas collected over 12 months and concentrated by polyethylene-glycol-precipitation, were analyzed by multiplex reverse-transcription-PCR for rotavirus/calicivirus/astrovirus and enterovirus/klassevirus/cosavirus, an...
Source: Virology - November 4, 2023 Category: Virology Authors: Alejandra Zamora-Figueroa Rita E Rosales Rixio Fern ández Viviana Ram írez Marjorie Bastardo Alba Far ías Esmeralda Vizzi Source Type: research
Considerations in engineering viral vectors for genome editing in plants
Virology. 2023 Oct 30;589:109922. doi: 10.1016/j.virol.2023.109922. Online ahead of print.ABSTRACTPlant viruses have been engineered to express proteins and induce gene silencing for decades. Recently, plant viruses have also been used to deliver components into plant cells for genome editing, a technique called virus-induced genome editing (VIGE). Although more than a dozen plant viruses have been engineered into VIGE vectors and VIGE has been successfully accomplished in some plant species, application of VIGE to crops that are difficult to tissue culture and/or have low regeneration efficiency is still tough. This paper...
Source: Virology - November 4, 2023 Category: Virology Authors: Xiaoyun Wu Ying Zhang Xue Jiang Tingshuai Ma Yating Guo Xiaoxia Wu Yushuang Guo Xiaofei Cheng Source Type: research
Detection and diversity of gastrointestinal viruses in wastewater from Caracas, Venezuela, 2021-2022
Virology. 2023 Oct 23;589:109913. doi: 10.1016/j.virol.2023.109913. Online ahead of print.ABSTRACTGastrointestinal viruses (GIV) are an important cause of childhood morbidity and mortality, particularly in developing countries. Their epidemiological impact in Venezuela during the COVID-19 pandemic remains unclear. GIV can also be detected in domestic sewage. Ninety-one wastewater samples from urban areas of Caracas collected over 12 months and concentrated by polyethylene-glycol-precipitation, were analyzed by multiplex reverse-transcription-PCR for rotavirus/calicivirus/astrovirus and enterovirus/klassevirus/cosavirus, an...
Source: Virology - November 4, 2023 Category: Virology Authors: Alejandra Zamora-Figueroa Rita E Rosales Rixio Fern ández Viviana Ram írez Marjorie Bastardo Alba Far ías Esmeralda Vizzi Source Type: research
Characterization of the marsupial endogenous retrovirus walb with a focus on satellite DNA formation
Virology. 2023 Oct 23;588:109911. doi: 10.1016/j.virol.2023.109911. Online ahead of print.ABSTRACTThe walbRep megasatellite DNA found in the red-necked wallaby was formed from the walb endogenous retrovirus. Our previous PCR experiments suggested the presence of walb and absence of walbRep in the genome of the tammar wallaby, which diverged from the red-necked wallaby 2-3 Mya. The results failed to exclude the possibility that certain walbRep sequences might have remained undetected owing to variation in the primer-annealing regions; therefore, the aforementioned suggestion was not confirmed. To obtain conclusive evidence,...
Source: Virology - November 2, 2023 Category: Virology Authors: Akihiko Koga Syed Farhan Ahmad Thitipong Panthum Worapong Singchat Kornsorn Srikulnath Source Type: research
