Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2024 Feb;590:109946. doi: 10.1016/j.virol.2023.109946. Epub 2023 Dec 11.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 between lo...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2024 Feb;590:109946. doi: 10.1016/j.virol.2023.109946. Epub 2023 Dec 11.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 between lo...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2024 Feb;590:109946. doi: 10.1016/j.virol.2023.109946. Epub 2023 Dec 11.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 between lo...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2024 Feb;590:109946. doi: 10.1016/j.virol.2023.109946. Epub 2023 Dec 11.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 between lo...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2024 Feb;590:109946. doi: 10.1016/j.virol.2023.109946. Epub 2023 Dec 11.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 between lo...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2023 Dec 11;590:109946. doi: 10.1016/j.virol.2023.109946. Online ahead of print.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 be...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2023 Dec 11;590:109946. doi: 10.1016/j.virol.2023.109946. Online ahead of print.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 be...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2023 Dec 11;590:109946. doi: 10.1016/j.virol.2023.109946. Online ahead of print.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 be...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2023 Dec 11;590:109946. doi: 10.1016/j.virol.2023.109946. Online ahead of print.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 be...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2023 Dec 11;590:109946. doi: 10.1016/j.virol.2023.109946. Online ahead of print.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 be...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Unveiling tetrahydroquinolines as promising BVDV entry inhibitors: Targeting the envelope protein
Virology. 2023 Dec 15;590:109968. doi: 10.1016/j.virol.2023.109968. Online ahead of print.ABSTRACTBovine viral diarrhea virus (BVDV) is known to cause financial losses and decreased productivity in the cattle industry worldwide. Currently, there are no available antiviral treatments for effectively controlling BVDV infections in laboratories or farms. The BVDV envelope protein (E2) mediates receptor recognition on the cell surface and is required for fusion of virus and cell membranes after the endocytic uptake of the virus during the entry process. Therefore, E2 is an attractive target for the development of antiviral str...
Source: Virology - December 23, 2023 Category: Virology Authors: Emilse S Leal Mar ía J Pascual Natalia S Adler Nicol ás Arrupe Fernando Merwaiss Luciana Giordano Daniela Fidalgo Diego Álvarez Mariela Bollini Source Type: research
Avian coronavirus infectious bronchitis virus Beaudette strain NSP9 interacts with STAT1 and inhibits its phosphorylation to facilitate viral replication
In this study, we uncovered a function of NSP9 in immune regulation. First, the host proteins that interacted with NSP9 were screened. The immune-related protein signal transducer and activator of transcription 1 (STAT1) was identified and the interaction between NSP9 and STAT1 was further confirmed. Furthermore, IBV replication was inhibited in STAT1-overexpressing cells but inversely affected in STAT1 knock-down cells. Importantly, NSP9 inhibited STAT1 phosphorylation. Finally, the expression of JAK/STAT pathway downstream genes IRF7 and ISG20 was significantly decreased in NSP9-overexpressing cells. These results showed...
Source: Virology - December 23, 2023 Category: Virology Authors: Ting Xia Shengkui Xu Xueyan Li Wenke Ruan Source Type: research
Unveiling tetrahydroquinolines as promising BVDV entry inhibitors: Targeting the envelope protein
Virology. 2023 Dec 15;590:109968. doi: 10.1016/j.virol.2023.109968. Online ahead of print.ABSTRACTBovine viral diarrhea virus (BVDV) is known to cause financial losses and decreased productivity in the cattle industry worldwide. Currently, there are no available antiviral treatments for effectively controlling BVDV infections in laboratories or farms. The BVDV envelope protein (E2) mediates receptor recognition on the cell surface and is required for fusion of virus and cell membranes after the endocytic uptake of the virus during the entry process. Therefore, E2 is an attractive target for the development of antiviral str...
Source: Virology - December 23, 2023 Category: Virology Authors: Emilse S Leal Mar ía J Pascual Natalia S Adler Nicol ás Arrupe Fernando Merwaiss Luciana Giordano Daniela Fidalgo Diego Álvarez Mariela Bollini Source Type: research
Avian coronavirus infectious bronchitis virus Beaudette strain NSP9 interacts with STAT1 and inhibits its phosphorylation to facilitate viral replication
In this study, we uncovered a function of NSP9 in immune regulation. First, the host proteins that interacted with NSP9 were screened. The immune-related protein signal transducer and activator of transcription 1 (STAT1) was identified and the interaction between NSP9 and STAT1 was further confirmed. Furthermore, IBV replication was inhibited in STAT1-overexpressing cells but inversely affected in STAT1 knock-down cells. Importantly, NSP9 inhibited STAT1 phosphorylation. Finally, the expression of JAK/STAT pathway downstream genes IRF7 and ISG20 was significantly decreased in NSP9-overexpressing cells. These results showed...
Source: Virology - December 23, 2023 Category: Virology Authors: Ting Xia Shengkui Xu Xueyan Li Wenke Ruan Source Type: research
Efficacy and tolerability of an mRNA vaccine expressing gB and pp38 antigens of Marek's disease virus in chickens
Virology. 2023 Dec 16;590:109970. doi: 10.1016/j.virol.2023.109970. Online ahead of print.ABSTRACTMarek's disease is a contagious proliferative disease of chickens caused by an alphaherpesvirus called Marek's disease virus. A bivalent mRNA vaccine encoding MDV's glycoprotein-B and phosphoprotein-38 antigens was synthesized and encapsulated in lipid nanoparticles. Tumor incidence, lesion score, organ weight indices, MDV genome load and cytokine expression were used to evaluate protection and immunostimulatory effects of the tested mRNA vaccine after two challenge trials. Results from the first trial showed decreased tumor i...
Source: Virology - December 22, 2023 Category: Virology Authors: Fatemeh Fazel Ayumi Matsuyama-Kato Mohammadali Alizadeh Nitish Boodhoo Shayan Sharif Source Type: research
