Distribution of human papillomavirus among Vietnamese women with cervical cancer and unusual genetic variability of HPV16
Virology. 2024 Mar 16;594:110058. doi: 10.1016/j.virol.2024.110058. Online ahead of print.ABSTRACTHPV16, with typical mutations that differ in geographical distribution and carcinogenic potency, has implications for cervical cancer screening, clinical diagnosis, and treatment. DNASTAR and MEGA were used to identify HPV16 variants and construct a phylogenetic tree. The most prevalent HPV genotypes were HPV16 (63.9%), HPV18 (26.7%), and other HPV (6.9%). HPV16 alterations were found in all E6, E7, and L1 genes, including 15 missense and 18 synonymous mutations. Missense mutations include R10G, Q14H, D25E, H78Y, L83V (E6); M2...
Source: Virology - March 23, 2024 Category: Virology Authors: Van Nam Le Van Nguyen Bang Le Xuan Son Hoang Van Duyet Le Source Type: research
Molecular characterization and pathogenicity of a novel monopartite geminivirus infecting tobacco in China
In this study, a novel geminivirus isolated from tobacco in Sichuan province of China, named tomato leaf curl Chuxiong virus (TLCCxV), was characterized by small RNA-based deep sequencing. The full-length of TLCCxV genome was determined to be 2744 nucleotides (nt) encoding six open reading frames. Phylogenetic and genome-wide pairwise identity analysis revealed that TLCCxV shared less than 91% identities with reported geminiviruses. A TLCCxV infectious clone was constructed and successfully infected Nicotiana benthamiana, N. tabacum, N. glutinosa, Solanum lycopersicum and Petunia hybrida plants. Furthermore, expression of ...
Source: Virology - March 22, 2024 Category: Virology Authors: Yuan Chen Shiping Guo Lianqiang Jiang Fangfang Yan Kaiqiang Hao Zhiping Wang Mengnan An Zihao Xia Fangfang Li Xueping Zhou Yuanhua Wu Source Type: research
Modulation of autophagy affected tumorigenesis induced by the envelope glycoprotein of JSRV
In conclusion, JSRV, through JSRV Env, takes advantage of the autophagy process, leading to the development of OPA.PMID:38518442 | DOI:10.1016/j.virol.2024.110059 (Source: Virology)
Source: Virology - March 22, 2024 Category: Virology Authors: Liang Zhang Hui Yang Xujie Duan Huiping Li Siriguleng Xu Hui Chen Jinlin Wang Yu Wang Shuying Liu Source Type: research
Increased expression of CD38 on endothelial cells in SARS-CoV-2 infection in cynomolgus macaques
Virology. 2024 Mar 14;594:110052. doi: 10.1016/j.virol.2024.110052. Online ahead of print.ABSTRACTSARS-CoV-2 infection causes activation of endothelial cells (ECs), leading to dysmorphology and dysfunction. To study the pathogenesis of endotheliopathy, the activation of ECs in lungs of cynomolgus macaques after SARS-CoV-2 infection and changes in nicotinamide adenine dinucleotide (NAD) metabolism in ECs were investigated, with a focus on the CD38 molecule, which degrades NAD in inflammatory responses after SARS-CoV-2 infection. Activation of ECs was seen from day 3 after SARS-CoV-2 infection in macaques, with increases of ...
Source: Virology - March 20, 2024 Category: Virology Authors: Cong Thanh Nguyen Misako Nakayama Hirohito Ishigaki Yoshinori Kitagawa Akemi Kakino Marumi Ohno Masashi Shingai Yasuhiko Suzuki Tatsuya Sawamura Hiroshi Kida Yasushi Itoh Source Type: research
Increased expression of CD38 on endothelial cells in SARS-CoV-2 infection in cynomolgus macaques
Virology. 2024 Mar 14;594:110052. doi: 10.1016/j.virol.2024.110052. Online ahead of print.ABSTRACTSARS-CoV-2 infection causes activation of endothelial cells (ECs), leading to dysmorphology and dysfunction. To study the pathogenesis of endotheliopathy, the activation of ECs in lungs of cynomolgus macaques after SARS-CoV-2 infection and changes in nicotinamide adenine dinucleotide (NAD) metabolism in ECs were investigated, with a focus on the CD38 molecule, which degrades NAD in inflammatory responses after SARS-CoV-2 infection. Activation of ECs was seen from day 3 after SARS-CoV-2 infection in macaques, with increases of ...
Source: Virology - March 20, 2024 Category: Virology Authors: Cong Thanh Nguyen Misako Nakayama Hirohito Ishigaki Yoshinori Kitagawa Akemi Kakino Marumi Ohno Masashi Shingai Yasuhiko Suzuki Tatsuya Sawamura Hiroshi Kida Yasushi Itoh Source Type: research
Increased expression of CD38 on endothelial cells in SARS-CoV-2 infection in cynomolgus macaques
Virology. 2024 Mar 14;594:110052. doi: 10.1016/j.virol.2024.110052. Online ahead of print.ABSTRACTSARS-CoV-2 infection causes activation of endothelial cells (ECs), leading to dysmorphology and dysfunction. To study the pathogenesis of endotheliopathy, the activation of ECs in lungs of cynomolgus macaques after SARS-CoV-2 infection and changes in nicotinamide adenine dinucleotide (NAD) metabolism in ECs were investigated, with a focus on the CD38 molecule, which degrades NAD in inflammatory responses after SARS-CoV-2 infection. Activation of ECs was seen from day 3 after SARS-CoV-2 infection in macaques, with increases of ...
Source: Virology - March 20, 2024 Category: Virology Authors: Cong Thanh Nguyen Misako Nakayama Hirohito Ishigaki Yoshinori Kitagawa Akemi Kakino Marumi Ohno Masashi Shingai Yasuhiko Suzuki Tatsuya Sawamura Hiroshi Kida Yasushi Itoh Source Type: research
Natural hyperoside extracted from hawthorn exhibits antiviral activity against porcine epidemic diarrhea virus in vitro and in vivo
Virology. 2024 Mar 11;594:110037. doi: 10.1016/j.virol.2024.110037. Online ahead of print.ABSTRACTPorcine epidemic diarrhea virus (PEDV) causes severe diarrhea and death in piglets, resulting in significant economic losses for the pork industry. There is an urgent need for new treatment strategies. Here, we focused on optimizing the process of purifying natural hyperoside (nHYP) from hawthorn and evaluating its effectiveness against PEDV both in vitro and in vivo. Our findings demonstrated that nHYP with a purity >98% was successfully isolated from hawthorn with an extraction rate of 0.42 mg/g. Furthermore, nHYP exhibit...
Source: Virology - March 18, 2024 Category: Virology Authors: Jun Wang Haibo Sun Mingjun Su Zijian Li Lu Li Feiyu Zhao Yongchen Zhang Wenfei Bai Shiping Yu Xu Yang Shanshan Qi Dan Yang Donghua Guo Chunqiu Li Qinghe Zhu Xiaoxu Xing Dongbo Sun Source Type: research
Natural hyperoside extracted from hawthorn exhibits antiviral activity against porcine epidemic diarrhea virus in vitro and in vivo
Virology. 2024 Mar 11;594:110037. doi: 10.1016/j.virol.2024.110037. Online ahead of print.ABSTRACTPorcine epidemic diarrhea virus (PEDV) causes severe diarrhea and death in piglets, resulting in significant economic losses for the pork industry. There is an urgent need for new treatment strategies. Here, we focused on optimizing the process of purifying natural hyperoside (nHYP) from hawthorn and evaluating its effectiveness against PEDV both in vitro and in vivo. Our findings demonstrated that nHYP with a purity >98% was successfully isolated from hawthorn with an extraction rate of 0.42 mg/g. Furthermore, nHYP exhibit...
Source: Virology - March 18, 2024 Category: Virology Authors: Jun Wang Haibo Sun Mingjun Su Zijian Li Lu Li Feiyu Zhao Yongchen Zhang Wenfei Bai Shiping Yu Xu Yang Shanshan Qi Dan Yang Donghua Guo Chunqiu Li Qinghe Zhu Xiaoxu Xing Dongbo Sun Source Type: research
Human parainfluenza virus type 2 V protein inhibits mitochondrial apoptosis pathway through two ways
Virology. 2024 Mar 11;594:110053. doi: 10.1016/j.virol.2024.110053. Online ahead of print.ABSTRACTParamyxoviruses are reported to block apoptosis for their replication, but the mechanisms remain unclear. Furthermore, regulation of mitochondrial apoptosis by paramyxoviruses has been hardly reported. We investigated whether and how human parainfluenza virus type 2 (hPIV-2) counteracts apoptosis. Infection of recombinant hPIV-2 carrying mutated V protein showed higher caspase 3/7 activity and higher cytochrome c release from mitochondria than wild type hPIV-2 infection. This indicates that V protein controls mitochondrial apo...
Source: Virology - March 16, 2024 Category: Virology Authors: Naoki Saka Machiko Nishio Keisuke Ohta Source Type: research
New perspective of small-molecule antiviral drugs development for RNA viruses
Virology. 2024 Mar 7;594:110042. doi: 10.1016/j.virol.2024.110042. Online ahead of print.ABSTRACTHigh variability and adaptability of RNA viruses allows them to spread between humans and animals, causing large-scale infectious diseases which seriously threat human and animal health and social development. At present, AIDS, viral hepatitis and other viral diseases with high incidence and low cure rate are still spreading around the world. The outbreaks of Ebola, Zika, dengue and in particular of the global pandemic of COVID-19 have presented serious challenges to the global public health system. The development of highly ef...
Source: Virology - March 16, 2024 Category: Virology Authors: Shasha Li Huixia Li Ruiya Lian Jingying Xie Ruofei Feng Source Type: research
Inhibition of PEDV viral entry upon blocking N-glycan elaboration
This study meticulously investigates the impact of O- and N-linked glycans on PEDV proteins and host cell interaction, shedding light on their influence on the virus's invasion process. Utilizing CRISPR-Cas9 technology to inhibit cell surface O- and N-linked glycan synthesis demonstrated no discernible impact on virus infection. However, progeny PEDV strains lacking these glycans exhibited a minor effect of O-linked glycans on virus infection. Conversely, a notable 40% reduction in infectivity was observed when the virus surface lacked N-linked glycans, emphasizing their pivotal role in facilitating virus recognition and b...
Source: Virology - March 16, 2024 Category: Virology Authors: Yong Zhao Tao Tang Wenchang Zhao Weiguang Fu Tao Li Source Type: research
Human parainfluenza virus type 2 V protein inhibits mitochondrial apoptosis pathway through two ways
Virology. 2024 Mar 11;594:110053. doi: 10.1016/j.virol.2024.110053. Online ahead of print.ABSTRACTParamyxoviruses are reported to block apoptosis for their replication, but the mechanisms remain unclear. Furthermore, regulation of mitochondrial apoptosis by paramyxoviruses has been hardly reported. We investigated whether and how human parainfluenza virus type 2 (hPIV-2) counteracts apoptosis. Infection of recombinant hPIV-2 carrying mutated V protein showed higher caspase 3/7 activity and higher cytochrome c release from mitochondria than wild type hPIV-2 infection. This indicates that V protein controls mitochondrial apo...
Source: Virology - March 16, 2024 Category: Virology Authors: Naoki Saka Machiko Nishio Keisuke Ohta Source Type: research
New perspective of small-molecule antiviral drugs development for RNA viruses
Virology. 2024 Mar 7;594:110042. doi: 10.1016/j.virol.2024.110042. Online ahead of print.ABSTRACTHigh variability and adaptability of RNA viruses allows them to spread between humans and animals, causing large-scale infectious diseases which seriously threat human and animal health and social development. At present, AIDS, viral hepatitis and other viral diseases with high incidence and low cure rate are still spreading around the world. The outbreaks of Ebola, Zika, dengue and in particular of the global pandemic of COVID-19 have presented serious challenges to the global public health system. The development of highly ef...
Source: Virology - March 16, 2024 Category: Virology Authors: Shasha Li Huixia Li Ruiya Lian Jingying Xie Ruofei Feng Source Type: research
Inhibition of PEDV viral entry upon blocking N-glycan elaboration
This study meticulously investigates the impact of O- and N-linked glycans on PEDV proteins and host cell interaction, shedding light on their influence on the virus's invasion process. Utilizing CRISPR-Cas9 technology to inhibit cell surface O- and N-linked glycan synthesis demonstrated no discernible impact on virus infection. However, progeny PEDV strains lacking these glycans exhibited a minor effect of O-linked glycans on virus infection. Conversely, a notable 40% reduction in infectivity was observed when the virus surface lacked N-linked glycans, emphasizing their pivotal role in facilitating virus recognition and b...
Source: Virology - March 16, 2024 Category: Virology Authors: Yong Zhao Tao Tang Wenchang Zhao Weiguang Fu Tao Li Source Type: research
Human parainfluenza virus type 2 V protein inhibits mitochondrial apoptosis pathway through two ways
Virology. 2024 Mar 11;594:110053. doi: 10.1016/j.virol.2024.110053. Online ahead of print.ABSTRACTParamyxoviruses are reported to block apoptosis for their replication, but the mechanisms remain unclear. Furthermore, regulation of mitochondrial apoptosis by paramyxoviruses has been hardly reported. We investigated whether and how human parainfluenza virus type 2 (hPIV-2) counteracts apoptosis. Infection of recombinant hPIV-2 carrying mutated V protein showed higher caspase 3/7 activity and higher cytochrome c release from mitochondria than wild type hPIV-2 infection. This indicates that V protein controls mitochondrial apo...
Source: Virology - March 16, 2024 Category: Virology Authors: Naoki Saka Machiko Nishio Keisuke Ohta Source Type: research
