Computational and AI-driven 3D structural analysis of human papillomavirus (HPV) oncoproteins E5, E6, and E7 reveal significant divergence of HPV E5 between low-risk and high-risk genotypes
Virology. 2023 Dec 11;590:109946. doi: 10.1016/j.virol.2023.109946. Online ahead of print.ABSTRACTThere are over 220 identified genotypes of Human papillomavirus (HPV), and the HPV genome encodes 3 major oncogenes, E5, E6, and E7. Conservation and divergence in protein sequence and function between low-risk versus high-risk oncogenic HPV genotypes has not been fully characterized. Here, we used modern computational and structural folding algorithms to perform a comparative analysis of HPV E5, E6, and E7 between multiple low risk and high risk genotypes. We first identified significantly greater sequence divergence in E5 be...
Source: Virology - December 26, 2023 Category: Virology Authors: Riley N Jones Sayuri Miyauchi Souvick Roy Nathalie Boutros Jyoti S Mayadev Loren K Mell Joseph A Califano Aldo Venuti Andrew B Sharabi Source Type: research
Unveiling tetrahydroquinolines as promising BVDV entry inhibitors: Targeting the envelope protein
Virology. 2023 Dec 15;590:109968. doi: 10.1016/j.virol.2023.109968. Online ahead of print.ABSTRACTBovine viral diarrhea virus (BVDV) is known to cause financial losses and decreased productivity in the cattle industry worldwide. Currently, there are no available antiviral treatments for effectively controlling BVDV infections in laboratories or farms. The BVDV envelope protein (E2) mediates receptor recognition on the cell surface and is required for fusion of virus and cell membranes after the endocytic uptake of the virus during the entry process. Therefore, E2 is an attractive target for the development of antiviral str...
Source: Virology - December 23, 2023 Category: Virology Authors: Emilse S Leal Mar ía J Pascual Natalia S Adler Nicol ás Arrupe Fernando Merwaiss Luciana Giordano Daniela Fidalgo Diego Álvarez Mariela Bollini Source Type: research
Avian coronavirus infectious bronchitis virus Beaudette strain NSP9 interacts with STAT1 and inhibits its phosphorylation to facilitate viral replication
In this study, we uncovered a function of NSP9 in immune regulation. First, the host proteins that interacted with NSP9 were screened. The immune-related protein signal transducer and activator of transcription 1 (STAT1) was identified and the interaction between NSP9 and STAT1 was further confirmed. Furthermore, IBV replication was inhibited in STAT1-overexpressing cells but inversely affected in STAT1 knock-down cells. Importantly, NSP9 inhibited STAT1 phosphorylation. Finally, the expression of JAK/STAT pathway downstream genes IRF7 and ISG20 was significantly decreased in NSP9-overexpressing cells. These results showed...
Source: Virology - December 23, 2023 Category: Virology Authors: Ting Xia Shengkui Xu Xueyan Li Wenke Ruan Source Type: research
Unveiling tetrahydroquinolines as promising BVDV entry inhibitors: Targeting the envelope protein
Virology. 2023 Dec 15;590:109968. doi: 10.1016/j.virol.2023.109968. Online ahead of print.ABSTRACTBovine viral diarrhea virus (BVDV) is known to cause financial losses and decreased productivity in the cattle industry worldwide. Currently, there are no available antiviral treatments for effectively controlling BVDV infections in laboratories or farms. The BVDV envelope protein (E2) mediates receptor recognition on the cell surface and is required for fusion of virus and cell membranes after the endocytic uptake of the virus during the entry process. Therefore, E2 is an attractive target for the development of antiviral str...
Source: Virology - December 23, 2023 Category: Virology Authors: Emilse S Leal Mar ía J Pascual Natalia S Adler Nicol ás Arrupe Fernando Merwaiss Luciana Giordano Daniela Fidalgo Diego Álvarez Mariela Bollini Source Type: research
Avian coronavirus infectious bronchitis virus Beaudette strain NSP9 interacts with STAT1 and inhibits its phosphorylation to facilitate viral replication
In this study, we uncovered a function of NSP9 in immune regulation. First, the host proteins that interacted with NSP9 were screened. The immune-related protein signal transducer and activator of transcription 1 (STAT1) was identified and the interaction between NSP9 and STAT1 was further confirmed. Furthermore, IBV replication was inhibited in STAT1-overexpressing cells but inversely affected in STAT1 knock-down cells. Importantly, NSP9 inhibited STAT1 phosphorylation. Finally, the expression of JAK/STAT pathway downstream genes IRF7 and ISG20 was significantly decreased in NSP9-overexpressing cells. These results showed...
Source: Virology - December 23, 2023 Category: Virology Authors: Ting Xia Shengkui Xu Xueyan Li Wenke Ruan Source Type: research
Efficacy and tolerability of an mRNA vaccine expressing gB and pp38 antigens of Marek's disease virus in chickens
Virology. 2023 Dec 16;590:109970. doi: 10.1016/j.virol.2023.109970. Online ahead of print.ABSTRACTMarek's disease is a contagious proliferative disease of chickens caused by an alphaherpesvirus called Marek's disease virus. A bivalent mRNA vaccine encoding MDV's glycoprotein-B and phosphoprotein-38 antigens was synthesized and encapsulated in lipid nanoparticles. Tumor incidence, lesion score, organ weight indices, MDV genome load and cytokine expression were used to evaluate protection and immunostimulatory effects of the tested mRNA vaccine after two challenge trials. Results from the first trial showed decreased tumor i...
Source: Virology - December 22, 2023 Category: Virology Authors: Fatemeh Fazel Ayumi Matsuyama-Kato Mohammadali Alizadeh Nitish Boodhoo Shayan Sharif Source Type: research
Efficacy and tolerability of an mRNA vaccine expressing gB and pp38 antigens of Marek's disease virus in chickens
Virology. 2023 Dec 16;590:109970. doi: 10.1016/j.virol.2023.109970. Online ahead of print.ABSTRACTMarek's disease is a contagious proliferative disease of chickens caused by an alphaherpesvirus called Marek's disease virus. A bivalent mRNA vaccine encoding MDV's glycoprotein-B and phosphoprotein-38 antigens was synthesized and encapsulated in lipid nanoparticles. Tumor incidence, lesion score, organ weight indices, MDV genome load and cytokine expression were used to evaluate protection and immunostimulatory effects of the tested mRNA vaccine after two challenge trials. Results from the first trial showed decreased tumor i...
Source: Virology - December 22, 2023 Category: Virology Authors: Fatemeh Fazel Ayumi Matsuyama-Kato Mohammadali Alizadeh Nitish Boodhoo Shayan Sharif Source Type: research
Polyphenol rich sugarcane extract (PRSE) has potential antiviral activity against influenza A virus in vitro
In conclusion, we show that PRSE displays antiviral activity against a broad range of IAV strains, in vitro.PMID:38118269 | DOI:10.1016/j.virol.2023.109969 (Source: Virology)
Source: Virology - December 20, 2023 Category: Virology Authors: Caolingzhi Tang Julio Carrera Montoya Svenja Fritzlar Matthew Flavel Sarah L Londrigan Jason M Mackenzie Source Type: research
Polyphenol rich sugarcane extract (PRSE) has potential antiviral activity against influenza A virus in vitro
In conclusion, we show that PRSE displays antiviral activity against a broad range of IAV strains, in vitro.PMID:38118269 | DOI:10.1016/j.virol.2023.109969 (Source: Virology)
Source: Virology - December 20, 2023 Category: Virology Authors: Caolingzhi Tang Julio Carrera Montoya Svenja Fritzlar Matthew Flavel Sarah L Londrigan Jason M Mackenzie Source Type: research
Polyphenol rich sugarcane extract (PRSE) has potential antiviral activity against influenza A virus in vitro
In conclusion, we show that PRSE displays antiviral activity against a broad range of IAV strains, in vitro.PMID:38118269 | DOI:10.1016/j.virol.2023.109969 (Source: Virology)
Source: Virology - December 20, 2023 Category: Virology Authors: Caolingzhi Tang Julio Carrera Montoya Svenja Fritzlar Matthew Flavel Sarah L Londrigan Jason M Mackenzie Source Type: research
Foot and mouth disease viruses are recurrently introduced to Israel and spread by extensively reared sheep and cattle: Insights from a whole-genome sequence analysis
Virology. 2023 Nov 24;590:109950. doi: 10.1016/j.virol.2023.109950. Online ahead of print.ABSTRACTDespite routine vaccination, Israel experiences recurrent outbreaks of foot and mouth disease (FMD). We analyzed VP1 coding sequences of viruses isolated during FMD outbreaks from 2001 to 2011 in Israel and neighboring nations. The Israeli strains were aligned with strains from neighboring countries in corresponding years, implying repeated FMD virus incursions. In 2007 a large FMD epidemic, caused by a serotype O virus, occurred in Israel. Bayesian analysis of whole-genome sequences of viruses isolated during this epidemic re...
Source: Virology - December 17, 2023 Category: Virology Authors: Greta Ivanov Eyal Klement Boris Gelman Ehud Elnekave Sharon Karniely Source Type: research
Foot and mouth disease viruses are recurrently introduced to Israel and spread by extensively reared sheep and cattle: Insights from a whole-genome sequence analysis
Virology. 2023 Nov 24;590:109950. doi: 10.1016/j.virol.2023.109950. Online ahead of print.ABSTRACTDespite routine vaccination, Israel experiences recurrent outbreaks of foot and mouth disease (FMD). We analyzed VP1 coding sequences of viruses isolated during FMD outbreaks from 2001 to 2011 in Israel and neighboring nations. The Israeli strains were aligned with strains from neighboring countries in corresponding years, implying repeated FMD virus incursions. In 2007 a large FMD epidemic, caused by a serotype O virus, occurred in Israel. Bayesian analysis of whole-genome sequences of viruses isolated during this epidemic re...
Source: Virology - December 17, 2023 Category: Virology Authors: Greta Ivanov Eyal Klement Boris Gelman Ehud Elnekave Sharon Karniely Source Type: research
Foot and mouth disease viruses are recurrently introduced to Israel and spread by extensively reared sheep and cattle: Insights from a whole-genome sequence analysis
Virology. 2023 Nov 24;590:109950. doi: 10.1016/j.virol.2023.109950. Online ahead of print.ABSTRACTDespite routine vaccination, Israel experiences recurrent outbreaks of foot and mouth disease (FMD). We analyzed VP1 coding sequences of viruses isolated during FMD outbreaks from 2001 to 2011 in Israel and neighboring nations. The Israeli strains were aligned with strains from neighboring countries in corresponding years, implying repeated FMD virus incursions. In 2007 a large FMD epidemic, caused by a serotype O virus, occurred in Israel. Bayesian analysis of whole-genome sequences of viruses isolated during this epidemic re...
Source: Virology - December 17, 2023 Category: Virology Authors: Greta Ivanov Eyal Klement Boris Gelman Ehud Elnekave Sharon Karniely Source Type: research
AMP-activated protein kinase (AMPK) suppresses Ibaraki virus propagation
Virology. 2023 Dec 2;590:109943. doi: 10.1016/j.virol.2023.109943. Online ahead of print.ABSTRACTThe Ibaraki virus (IBAV) causes Ibaraki disease in cattle. Our previous studies have shown that IBAV uses macropinocytosis to enter the host cell and exit from the endosome to the cytosol in response to endosomal acidification. To further explore the mechanism of IBAV infection and replication, we examined the effect of inhibitors of mitochondrial oxidative phosphorylation, carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and antimycin A, on IBAV propagation. These inhibitors significantly suppressed IBAV propagation, with redu...
Source: Virology - December 16, 2023 Category: Virology Authors: Kiichi Ohkubo Shusaku Shibutani Hiroyuki Iwata Source Type: research
Epidermal growth factor receptor-dependent stimulation of differentiation by human papillomavirus type 16 E5
Virology. 2023 Dec 12;590:109952. doi: 10.1016/j.virol.2023.109952. Online ahead of print.ABSTRACTHuman papillomaviruses (HPVs) infect keratinocytes of stratified squamous epithelia, and persistent infection with high-risk HPV types, such as HPV16, may lead to the development of malignancies. HPV evades host immunity in part by linking its gene expression to the host differentiation program, and therefore relies on differentiation to complete its life cycle. Based on previous reports indicating that the HPV16 protein E5 is important in the late stages of the differentiation-dependent life cycle, we found that organotypic c...
Source: Virology - December 16, 2023 Category: Virology Authors: Jessica Trammel Oluwamuyiwa Amusan Allison Hultgren Gaurav Raikhy Jason M Bodily Source Type: research
