Alum/CpG adjuvant promotes immunogenicity of inactivated SARS-CoV-2 Omicron vaccine through enhanced humoral and cellular immunity
Virology. 2024 Mar 7;594:110050. doi: 10.1016/j.virol.2024.110050. Online ahead of print.ABSTRACTThe SARS-CoV-2 Omicron variant, which was classified as a variant of concern (VOC) by the World Health Organization on 26 November 2021, has attracted worldwide attention for its high transmissibility and immune evasion ability. The existing COVID-19 vaccine has been shown to be less effective in preventing Omicron variant infection and symptomatic infection, which brings new challenges to vaccine development and application. Here, we evaluated the immunogenicity and safety of an Omicron variant COVID-19 inactivated vaccine con...
Source: Virology - March 13, 2024 Category: Virology Authors: Xiaotong Zheng Rong Yang Yuxiu Zhao Yadan Zhang Guangying Yuan Weidong Li Zhuangzhuang Xiao Xiaofei Dong Meng Ma Yancen Guo Wei Wang Xue Zhao Hongqiang Yang Shaoting Qiu Zheng Peng Ankang Liu Shouzhi Yu Yuntao Zhang Source Type: research
Distinct tomato yellow leaf curl Chuxiong virus isolated from whiteflies and plants in China and its symptom determinant and suppressor of post-transcriptional gene silencing
Virology. 2024 Mar 5;594:110040. doi: 10.1016/j.virol.2024.110040. Online ahead of print.ABSTRACTA begomovirus isolated from whiteflies (Bemisia tabaci) and tomato, sweet potato in China was found to be representative of a distinct begomovirus species, for which the name tomato yellow leaf curl Chuxiong virus (TYLCCxV) is proposed. The results of genomic identification and sequence comparison showed that TYLCCxV shares the highest complete nucleotide sequence identity (88.3%) with croton yellow vein mosaic virus (CroYVMV), and may have originated from the recombination between synedrella leaf curl virus (SyLCV) and squash ...
Source: Virology - March 12, 2024 Category: Virology Authors: Yan Xie Xianan Liu Chaohu Luo Qianqian Hu Xuan Che Liling Zhao Min Zhao Liqi Wu Ming Ding Source Type: research
Characterization of Sf9 cell clones with differential susceptibilities to Sf-rhabdovirus X < sup > +3.7 < /sup > and Sf-rhabdovirus X < sup > - < /sup > replication
Virology. 2024 Feb 27;594:110038. doi: 10.1016/j.virol.2024.110038. Online ahead of print.ABSTRACTOur laboratory previously discovered a novel rhabdovirus in the Spodoptera frugiperda Sf9 insect cell line that was designated as Sf-rhabdovirus. Using limiting dilution, this cell line was found to be a mixed population of cells infected by Sf-rhabdovirus variants containing either the full length X accessory gene with a 3.7 kb internal duplication (designated as Sf-rhabdovirus X+3.7) or lacking the duplication and part of the X gene (designated as Sf-rhabdovirus X-), and cells that were negative for Sf-rhabdovirus. In this p...
Source: Virology - March 12, 2024 Category: Virology Authors: Hailun Ma Andrea Kennard Nicholas Mattson Arifa S Khan Source Type: research
Transcriptome analysis of the synergistic mechanisms between two strains of potato virus Y in Solanum tuberosum L
In this study, we performed a comparative transcriptomic analysis of potato (Solanum tuberosum L.) leaves. The strains PVYN-Wi-HLJ-BDH-2 and PVYNTN-NW-INM-W-369-12 are henceforth designated as BDH and 369, respectively. In total, 806 differentially expressed genes (DEGs) were detected between the Control and JZ (preinfected with BDH and challenge with 369) treatment. Gene Ontology (GO) analysis showed that the response to external biological stimulation, signal transduction, kinase, immunity, redox pathways were significantly enriched. Among these pathways, we identified numerous differentially expressed metabolites relate...
Source: Virology - March 11, 2024 Category: Virology Authors: Liping Xu Wei Zhang Shangwu Liu Yanling Gao Yuanju Huang Xianzhou Nie Yanju Bai Source Type: research
Transcriptome analysis of the synergistic mechanisms between two strains of potato virus Y in Solanum tuberosum L
In this study, we performed a comparative transcriptomic analysis of potato (Solanum tuberosum L.) leaves. The strains PVYN-Wi-HLJ-BDH-2 and PVYNTN-NW-INM-W-369-12 are henceforth designated as BDH and 369, respectively. In total, 806 differentially expressed genes (DEGs) were detected between the Control and JZ (preinfected with BDH and challenge with 369) treatment. Gene Ontology (GO) analysis showed that the response to external biological stimulation, signal transduction, kinase, immunity, redox pathways were significantly enriched. Among these pathways, we identified numerous differentially expressed metabolites relate...
Source: Virology - March 11, 2024 Category: Virology Authors: Liping Xu Wei Zhang Shangwu Liu Yanling Gao Yuanju Huang Xianzhou Nie Yanju Bai Source Type: research
A comparative analysis of phage classification methods in light of the recent ICTV taxonomic revisions
This study compares six categorization tools and establishes a novel multi-method approach, combining genome similarity and specialized protein analysis. Applying the method to APEC phage P151 showed consistent categorization across platforms. A possible workflow for phage classification is proposed; offering a versatile tool for phage research and development.PMID:38461619 | DOI:10.1016/j.virol.2024.110016 (Source: Virology)
Source: Virology - March 10, 2024 Category: Virology Authors: Xihui Zhu Luqi Tang Zhiwei Wang Feng Xie Wei Zhang Yubao Li Source Type: research
A comparative analysis of phage classification methods in light of the recent ICTV taxonomic revisions
This study compares six categorization tools and establishes a novel multi-method approach, combining genome similarity and specialized protein analysis. Applying the method to APEC phage P151 showed consistent categorization across platforms. A possible workflow for phage classification is proposed; offering a versatile tool for phage research and development.PMID:38461619 | DOI:10.1016/j.virol.2024.110016 (Source: Virology)
Source: Virology - March 10, 2024 Category: Virology Authors: Xihui Zhu Luqi Tang Zhiwei Wang Feng Xie Wei Zhang Yubao Li Source Type: research
Saccharomyces cerevisiae cells that display norovirus P induce both systemic and mucosal neutralizing antibodies
In this study, we constructed a recombinant Saccharomyces cerevisiae (S. cerevisiae) expressing the HuNov P protein (GII. 4) and evaluated its immunogenicity in mice after oral delivery. First, the recombinant S. cerevisiae (EBY100/pYD1-P) efficiently expressed P, as evidenced by western blotting and indirect fluorescent assay. Second, after oral administration, EBY100/pYD1-P, especially the high-dose group (5 × 109 clone formation units), elicited systemic and mucosal immune responses characterized by significant sera IgG, IgA, and mucosal sIgA. More importantly, these antibodies showed a substantial neutralization effec...
Source: Virology - March 9, 2024 Category: Virology Authors: Xin He Nianzhu Jiang Yaoming Li Source Type: research
Saccharomyces cerevisiae cells that display norovirus P induce both systemic and mucosal neutralizing antibodies
In this study, we constructed a recombinant Saccharomyces cerevisiae (S. cerevisiae) expressing the HuNov P protein (GII. 4) and evaluated its immunogenicity in mice after oral delivery. First, the recombinant S. cerevisiae (EBY100/pYD1-P) efficiently expressed P, as evidenced by western blotting and indirect fluorescent assay. Second, after oral administration, EBY100/pYD1-P, especially the high-dose group (5 × 109 clone formation units), elicited systemic and mucosal immune responses characterized by significant sera IgG, IgA, and mucosal sIgA. More importantly, these antibodies showed a substantial neutralization effec...
Source: Virology - March 9, 2024 Category: Virology Authors: Xin He Nianzhu Jiang Yaoming Li Source Type: research
Oncolytic viral therapy for gliomas: Advances in the mechanisms and approaches to delivery
Virology. 2024 Feb 20;593:110033. doi: 10.1016/j.virol.2024.110033. Online ahead of print.ABSTRACTGlioma is a diverse category of tumors originating from glial cells encompasses various subtypes, based on the specific type of glial cells involved. The most aggressive is glioblastoma multiforme (GBM), which stands as the predominant primary malignant tumor within the central nervous system in adults. Despite the application of treatment strategy, the median survival rate for GBM patients still hovers around 15 months. Oncolytic viruses (OVs) are artificially engineered viruses designed to selectively target and induce apopt...
Source: Virology - March 5, 2024 Category: Virology Authors: A Romanishin A Vasilev E Khasanshin A Evtekhov E Pusynin K Rubina V Kakotkin M Agapov E Semina Source Type: research
Oncolytic viral therapy for gliomas: Advances in the mechanisms and approaches to delivery
Virology. 2024 Feb 20;593:110033. doi: 10.1016/j.virol.2024.110033. Online ahead of print.ABSTRACTGlioma is a diverse category of tumors originating from glial cells encompasses various subtypes, based on the specific type of glial cells involved. The most aggressive is glioblastoma multiforme (GBM), which stands as the predominant primary malignant tumor within the central nervous system in adults. Despite the application of treatment strategy, the median survival rate for GBM patients still hovers around 15 months. Oncolytic viruses (OVs) are artificially engineered viruses designed to selectively target and induce apopt...
Source: Virology - March 5, 2024 Category: Virology Authors: A Romanishin A Vasilev E Khasanshin A Evtekhov E Pusynin K Rubina V Kakotkin M Agapov E Semina Source Type: research
Oncolytic viral therapy for gliomas: Advances in the mechanisms and approaches to delivery
Virology. 2024 Feb 20;593:110033. doi: 10.1016/j.virol.2024.110033. Online ahead of print.ABSTRACTGlioma is a diverse category of tumors originating from glial cells encompasses various subtypes, based on the specific type of glial cells involved. The most aggressive is glioblastoma multiforme (GBM), which stands as the predominant primary malignant tumor within the central nervous system in adults. Despite the application of treatment strategy, the median survival rate for GBM patients still hovers around 15 months. Oncolytic viruses (OVs) are artificially engineered viruses designed to selectively target and induce apopt...
Source: Virology - March 5, 2024 Category: Virology Authors: A Romanishin A Vasilev E Khasanshin A Evtekhov E Pusynin K Rubina V Kakotkin M Agapov E Semina Source Type: research
Preliminary report on therapeutic potential of coal-derived carbon quantum dots against SARS-CoV-2 virus
Virology. 2024 Feb 27;593:110036. doi: 10.1016/j.virol.2024.110036. Online ahead of print.ABSTRACTDue to the pandemic of COVID-19 and subsequent emerging of new mutant strains, there has been a worldwide hunt for therapeutic and protective agents for its inhibition. In this short communication, for the first time, we report the coal-derived carbon quantum dot (CQD) for the possible therapeutic application against SARS-CoV-2. The synthesized C1-CQD is observed to be safe towards the normal cell line at highest dose, while effectively inhibiting growth of SARS-CoV2 (>95%) with IC50 value of 5.469 μg/mL. Moreover, C1-CQD ...
Source: Virology - March 3, 2024 Category: Virology Authors: Binoy K Saikia Kallol Roy Rituraj Konwar Source Type: research
Preliminary report on therapeutic potential of coal-derived carbon quantum dots against SARS-CoV-2 virus
Virology. 2024 Feb 27;593:110036. doi: 10.1016/j.virol.2024.110036. Online ahead of print.ABSTRACTDue to the pandemic of COVID-19 and subsequent emerging of new mutant strains, there has been a worldwide hunt for therapeutic and protective agents for its inhibition. In this short communication, for the first time, we report the coal-derived carbon quantum dot (CQD) for the possible therapeutic application against SARS-CoV-2. The synthesized C1-CQD is observed to be safe towards the normal cell line at highest dose, while effectively inhibiting growth of SARS-CoV2 (>95%) with IC50 value of 5.469 μg/mL. Moreover, C1-CQD ...
Source: Virology - March 3, 2024 Category: Virology Authors: Binoy K Saikia Kallol Roy Rituraj Konwar Source Type: research
Preliminary report on therapeutic potential of coal-derived carbon quantum dots against SARS-CoV-2 virus
Virology. 2024 Feb 27;593:110036. doi: 10.1016/j.virol.2024.110036. Online ahead of print.ABSTRACTDue to the pandemic of COVID-19 and subsequent emerging of new mutant strains, there has been a worldwide hunt for therapeutic and protective agents for its inhibition. In this short communication, for the first time, we report the coal-derived carbon quantum dot (CQD) for the possible therapeutic application against SARS-CoV-2. The synthesized C1-CQD is observed to be safe towards the normal cell line at highest dose, while effectively inhibiting growth of SARS-CoV2 (>95%) with IC50 value of 5.469 μg/mL. Moreover, C1-CQD ...
Source: Virology - March 3, 2024 Category: Virology Authors: Binoy K Saikia Kallol Roy Rituraj Konwar Source Type: research
