Development of full-length infectious cDNA clones and host range identification of an echinacea strain of tobacco streak virus
Virology. 2024 Feb 11;593:110013. doi: 10.1016/j.virol.2024.110013. Online ahead of print.ABSTRACTTobacco streak virus induces severe diseases on a wide range of plants and becomes an emerging threat to crop yields. However, the infectious clones of TSV remain to be developed for reverse genetics studies. Here, we obtained the full genome sequence of a TSV-CNB isolate and analyzed the phylogenetic characteristics. Subsequently, we developed the full-length infectious cDNA clones of TSV-CNB driven by 35 S promoter using yeast homologous recombination. Furthermore, the host range of TSV-CNB isolate was determined by Agrobact...
Source: Virology - February 19, 2024 Category: Virology Authors: Qi Liu Menglin Li Xuanyu Dong Chengxiao Zuo Zhangyao Nie Zongying Zhang Cheng-Gui Han Ying Wang Source Type: research
Mucosal vaccines for viral diseases: Status and prospects
Virology. 2024 Feb 15;593:110026. doi: 10.1016/j.virol.2024.110026. Online ahead of print.ABSTRACTVirus-associated infectious diseases are highly detrimental to human health and animal husbandry. Among all countermeasures against infectious diseases, prophylactic vaccines, which developed through traditional or novel approaches, offer potential benefits. More recently, mucosal vaccines attract attention for their extraordinary characteristics compared to conventional parenteral vaccines, particularly for mucosal-related pathogens. Representatively, coronavirus disease 2019 (COVID-19), a respiratory disease caused by severe...
Source: Virology - February 19, 2024 Category: Virology Authors: Bingjie Ma Mengxiao Tao Zhili Li Quanfang Zheng Haigang Wu Peirong Chen Source Type: research
Genome analysis of a novel avian atadenovirus reveals a possible horizontal gene transfer
We report the discovery and characterization of a novel adenovirus, Zoothera dauma adenovirus (ZdAdV), from a wild bird species, Zoothera dauma (Scaly thrush). This new atadenovirus was discovered by metagenomic sequencing without virus cultivation. Analyses of the full genome sequence revealed that this new virus is a distinct member of the genus Atadenovirus and represents a novel species. ZdAdV has a genome of 34,760 bp with 28 predicted genes and 39% GC content. ZdAdV is the first atadenovirus to contain ORF19, a gene previously found only in aviadenoviruses. Phylogenetic analysis of ORF19 suggests that it was acquired...
Source: Virology - February 18, 2024 Category: Virology Authors: Weibo Zheng Xiaopeng Teng Tingshu Jiang Wenli Tang Linlin Jiang Hongwei Zhu Xin Yu Guozhong Chen Jiao Wang Jianlong Zhang Mingjuan Qu Xingxiao Zhang Source Type: research
p53 suppresses the inflammatory response following respiratory syncytial virus infection by inhibiting TLR2
This study aimed to explore the correlation between p53 and Toll-like receptors (TLRs) post RSV infection. p53 levels exhibited a substantial decrease in nasopharyngeal aspirates (NPAs) from infants with RSV infection compared to control group. Manipulating p53 expression had no significant impact on RSV replication or interferon signaling pathway. Suppression of p53 expression led to heightened inflammation following RSV infection in A549 cells or airways of BALB/c mice. while stabilizing p53 expression using Nutlin-3a mitigated the inflammatory response in A549 cells. Additionally, Inhibiting p53 expression significantly...
Source: Virology - February 18, 2024 Category: Virology Authors: Jiao Liu Leiqiong Gao Na Zhou Zhenghong Jiang Siyi Che Yu Deng Na Zang Luo Ren Xiaohong Xie Jun Xie Enmei Liu Source Type: research
Genome analysis of a novel avian atadenovirus reveals a possible horizontal gene transfer
We report the discovery and characterization of a novel adenovirus, Zoothera dauma adenovirus (ZdAdV), from a wild bird species, Zoothera dauma (Scaly thrush). This new atadenovirus was discovered by metagenomic sequencing without virus cultivation. Analyses of the full genome sequence revealed that this new virus is a distinct member of the genus Atadenovirus and represents a novel species. ZdAdV has a genome of 34,760 bp with 28 predicted genes and 39% GC content. ZdAdV is the first atadenovirus to contain ORF19, a gene previously found only in aviadenoviruses. Phylogenetic analysis of ORF19 suggests that it was acquired...
Source: Virology - February 18, 2024 Category: Virology Authors: Weibo Zheng Xiaopeng Teng Tingshu Jiang Wenli Tang Linlin Jiang Hongwei Zhu Xin Yu Guozhong Chen Jiao Wang Jianlong Zhang Mingjuan Qu Xingxiao Zhang Source Type: research
p53 suppresses the inflammatory response following respiratory syncytial virus infection by inhibiting TLR2
This study aimed to explore the correlation between p53 and Toll-like receptors (TLRs) post RSV infection. p53 levels exhibited a substantial decrease in nasopharyngeal aspirates (NPAs) from infants with RSV infection compared to control group. Manipulating p53 expression had no significant impact on RSV replication or interferon signaling pathway. Suppression of p53 expression led to heightened inflammation following RSV infection in A549 cells or airways of BALB/c mice. while stabilizing p53 expression using Nutlin-3a mitigated the inflammatory response in A549 cells. Additionally, Inhibiting p53 expression significantly...
Source: Virology - February 18, 2024 Category: Virology Authors: Jiao Liu Leiqiong Gao Na Zhou Zhenghong Jiang Siyi Che Yu Deng Na Zang Luo Ren Xiaohong Xie Jun Xie Enmei Liu Source Type: research
Preliminary molecular study for DIVA trial of antigenically characterized circulating bovine herpesvirus subtype 1.1 in Egypt
In this study, multiplex real-time PCR targeting gD and gE genes was applied for BoHV-1 screening on 60 clinical samples from cattle with a history of vaccination, in some cases by US2-deleted marker vaccines, that were suffering from severe respiratory symptoms. Conventional PCR targeting the gC and US2 flanking region was done for molecular characterization and identification of the US2-deleted vaccine strain. Six samples were positive for BoHV-1 by both RT-PCR and conventional PCR. Surprisingly, a conventional PCR DIVA trial based on the US2 gene revealed that only one sample that exhibited the US2 gene was a wild virus...
Source: Virology - February 17, 2024 Category: Virology Authors: Basel A Abukhadra Sahar Abd El Rahman Mohamed A Soltan Giuma E Elhafi Samah M Mosad Source Type: research
Meta-transcriptomic identification of groundnut RNA viruses in western Kenya and the novel detection of groundnut as a host for Cauliflower mosaic virus
CONCLUSION: Our findings demonstrate the power of Next Generation Sequencing in the discovery and identification of novel viruses in groundnuts. The detection of the new viruses indicates the complexity of virus diseases in groundnuts and would require more focus in future studies to establish the effect of the viruses as sole or mixed infections on the crop. The detection of PeMoV with potential origin from Malawi indicates the importance of seed certification and cross-boundary seed health testing.PMID:38367474 | DOI:10.1016/j.virol.2024.110011 (Source: Virology)
Source: Virology - February 17, 2024 Category: Virology Authors: Dennis Obonyo George Ouma Rachel Ikawa Damaris A Odeny Source Type: research
Engineering of stable infectious cDNA constructs of a fluorescently tagged tomato chlorosis virus
This study demonstrates the potential of ToCV-GFP as a valuable tool for the visual tracking of infection and movement of criniviruses in living plants.PMID:38364352 | DOI:10.1016/j.virol.2024.110010 (Source: Virology)
Source: Virology - February 16, 2024 Category: Virology Authors: Sun-Jung Kwon Ye-Ji Lee Young-Eun Cho Hee-Seong Byun Jang-Kyun Seo Source Type: research
Engineering of stable infectious cDNA constructs of a fluorescently tagged tomato chlorosis virus
This study demonstrates the potential of ToCV-GFP as a valuable tool for the visual tracking of infection and movement of criniviruses in living plants.PMID:38364352 | DOI:10.1016/j.virol.2024.110010 (Source: Virology)
Source: Virology - February 16, 2024 Category: Virology Authors: Sun-Jung Kwon Ye-Ji Lee Young-Eun Cho Hee-Seong Byun Jang-Kyun Seo Source Type: research
Distribution patterns and functional diversity of DNA viruses determined by ecological niches in huge river ecosystems
Virology. 2024 Feb 11;593:110015. doi: 10.1016/j.virol.2024.110015. Online ahead of print.ABSTRACTWhile the vast number of DNA and RNA viruses participate in biogeochemical cycles in natural systems, little is known about virome in river ecosystems. Here, we analyzed the DNA viral composition and its metabolic potential in the Yangtze River, including freshwater (FW) and freshwater sediments (FWS). A total of 1237 river-derived virus contigs (RVCs) were obtained following de novo assembly from 62 metagenomics. We found that the viral diversity is significantly positively correlated longitudinally. Moreover, FW exhibited a ...
Source: Virology - February 15, 2024 Category: Virology Authors: Kang Zhou Hui Zhou Source Type: research
Distribution patterns and functional diversity of DNA viruses determined by ecological niches in huge river ecosystems
Virology. 2024 Feb 11;593:110015. doi: 10.1016/j.virol.2024.110015. Online ahead of print.ABSTRACTWhile the vast number of DNA and RNA viruses participate in biogeochemical cycles in natural systems, little is known about virome in river ecosystems. Here, we analyzed the DNA viral composition and its metabolic potential in the Yangtze River, including freshwater (FW) and freshwater sediments (FWS). A total of 1237 river-derived virus contigs (RVCs) were obtained following de novo assembly from 62 metagenomics. We found that the viral diversity is significantly positively correlated longitudinally. Moreover, FW exhibited a ...
Source: Virology - February 15, 2024 Category: Virology Authors: Kang Zhou Hui Zhou Source Type: research
Australian terrestrial environments harbour extensive RNA virus diversity
Virology. 2024 Feb 8;593:110007. doi: 10.1016/j.virol.2024.110007. Online ahead of print.ABSTRACTAustralia is home to a diverse range of unique native fauna and flora. To address whether Australian ecosystems also harbour unique viruses, we performed meta-transcriptomic sequencing of 16 farmland and sediment samples taken from the east and west coasts of Australia. We identified 2460 putatively novel RNA viruses across 18 orders, the vast majority of which belonged to the microbe-associated phylum Lenarviricota. In many orders, such as the Nodamuvirales and Ghabrivirales, the novel viruses identified here comprised entirel...
Source: Virology - February 12, 2024 Category: Virology Authors: Sabrina Sadiq Erin Harvey Jonathon C O Mifsud Budiman Minasny Alex B McBratney Liana E Pozza Jackie E Mahar Edward C Holmes Source Type: research
Proprotein convertase cleavage of Ictalurid herpesvirus 1 spike-like protein ORF46 is modulated by N-glycosylation
Virology. 2024 Feb 6;592:110008. doi: 10.1016/j.virol.2024.110008. Online ahead of print.ABSTRACTViral spike proteins undergo a special maturation process that enables host cell receptor recognition, membrane fusion, and viral entry, facilitating effective virus infection. Here, we investigated the protease cleavage features of ORF46, a spike-like protein in Ictalurid herpesvirus 1 (IcHV-1) sharing similarity with spikes of Nidovirales members. We noted that during cleavage, full-length ORF46 is cleaved into ∼55-kDa and ∼100-kDa subunits. Moreover, truncation or site-directed mutagenesis at the recognition sites of pro...
Source: Virology - February 9, 2024 Category: Virology Authors: Fei Yu Jiehua Xu Hongxun Chen Siyang Song Chunlan Nie Kai Hao Zhe Zhao Source Type: research
Isolation and characterization of genotype 4 Eurasian avian-like H1N1 influenza virus in pigs suffering from pneumonia
Virology. 2024 Feb 2;592:110009. doi: 10.1016/j.virol.2024.110009. Online ahead of print.ABSTRACTSwine influenza viruses pose ongoing threat to pork industry throughout the world. In 2023, fattening pigs from a swine farm in Inner Mongolia of China experienced influenza-like symptoms. Co-infection of influenza A virus with Pasteurella multocida was diagnosed in lung tissues of diseased pigs and a genotype 4 (G4) Eurasian avian-like (EA) H1N1 virus was isolated, which was named as A/swine/Neimenggu/0326/2023. We demonstrated the virus preferentially bound human-like SAα2,6Gal receptor. It was noteworthy that the virus poss...
Source: Virology - February 8, 2024 Category: Virology Authors: Jizhe Yang Riguo Lan Haoyu Chang Han Li Haili Yu Qi Tong Jinhua Liu Honglei Sun Source Type: research
