Pseudotyped zoonotic thogotoviruses exhibit broad entry range in mammalian cells

This study therefore developed a cell-cell fusion assay and three pseudotyping tools and used them to assess envelope function and cell tropism. Envelope glycoproteins of Dhori (DHOV), Thogoto (THOV), Bourbon, and Sinu viruses were all revealed to exhibit pH-dependent triggering of membrane fusion. Lentivirus vectors were robustly pseudotyped with these glycoproteins while influenza virus vectors showed pseudotyping compatibility, albeit at lower efficiencies. Replication-competent vesicular stomatitis virus expressing DHOV or THOV glycoproteins were also successfully generated. These pseudotyped viruses mediated entry into a wide range of mammalian cell lines, including human primary cells. The promiscuousness of these viruses suggests the use of a relatively ubiquitous receptor and their entry into numerous mammalian cells emphasize their high potential as veterinary and zoonotic diseases.PMID:37931589 | DOI:10.1016/j.virol.2023.109914

Source: Virology - November 6, 2023 Category: Virology Authors: Thiprampai Thamamongood Juggragarn Jengarn Papon Muangsanit Nalinrat Petpiroon Kanjana Srisutthisamphan Khemphitcha Attasombat Ratjika Wongwanakul Sasitorn Aueviriyavit Sira Laohathai Anan Jongkaewwattana Samaporn Teeravechyan Source Type: research