Dissecting the biophysics and biology of intrinsically disordered proteins
Trends Biochem Sci. 2023 Nov 8:S0968-0004(23)00257-8. doi: 10.1016/j.tibs.2023.10.002. Online ahead of print.ABSTRACTIntrinsically disordered regions (IDRs) within human proteins play critical roles in cellular information processing, including signaling, transcription, stress response, DNA repair, genome organization, and RNA processing. Here, we summarize current challenges in the field and propose cutting-edge approaches to address them in physiology and disease processes, with a focus on cancer.PMID:37949765 | DOI:10.1016/j.tibs.2023.10.002 (Source: Trends in Biochemical Sciences)
Source: Trends in Biochemical Sciences - November 10, 2023 Category: Biochemistry Authors: Priya R Banerjee Alex S Holehouse Richard Kriwacki Paul Robustelli Hao Jiang Alexander I Sobolevsky Jennifer M Hurley Joshua T Mendell Source Type: research
Type III CRISPR-Cas: beyond the Cas10 effector complex
Trends Biochem Sci. 2023 Nov 8:S0968-0004(23)00261-X. doi: 10.1016/j.tibs.2023.10.006. Online ahead of print.ABSTRACTType III CRISPR-Cas loci encode some of the most abundant, yet complex, immune systems of prokaryotes. They are composed of a Cas10 complex that uses an RNA guide to recognize transcripts from bacteriophage and plasmid invaders. Target recognition triggers three activities within this complex: ssDNA degradation, synthesis of cyclic oligoadenylates (cOA) that act as second messengers to activate CARF-domain effectors, and cleavage of target RNA. This review covers recent research in type III CRISPR-Cas system...
Source: Trends in Biochemical Sciences - November 10, 2023 Category: Biochemistry Authors: Gianna Stella Luciano Marraffini Source Type: research
Wrapping up a special series on scientific figure development
Trends Biochem Sci. 2023 Dec;48(12):1007-1008. doi: 10.1016/j.tibs.2023.09.008.NO ABSTRACTPMID:37949051 | DOI:10.1016/j.tibs.2023.09.008 (Source: Trends in Biochemical Sciences)
Source: Trends in Biochemical Sciences - November 10, 2023 Category: Biochemistry Authors: Sannie Culbertson Source Type: research
Turning science into cover art
Trends Biochem Sci. 2023 Dec;48(12):1009-1011. doi: 10.1016/j.tibs.2023.09.006.NO ABSTRACTPMID:37949052 | DOI:10.1016/j.tibs.2023.09.006 (Source: Trends in Biochemical Sciences)
Source: Trends in Biochemical Sciences - November 10, 2023 Category: Biochemistry Authors: Helen Dauben Ivan Matic Rune Thomas Kidmose Bj ørn Panyella Pedersen Tannishtha Saha Michela Di Virgilio Julie Ho Sung Source Type: research
Dissecting the biophysics and biology of intrinsically disordered proteins
Trends Biochem Sci. 2023 Nov 8:S0968-0004(23)00257-8. doi: 10.1016/j.tibs.2023.10.002. Online ahead of print.ABSTRACTIntrinsically disordered regions (IDRs) within human proteins play critical roles in cellular information processing, including signaling, transcription, stress response, DNA repair, genome organization, and RNA processing. Here, we summarize current challenges in the field and propose cutting-edge approaches to address them in physiology and disease processes, with a focus on cancer.PMID:37949765 | DOI:10.1016/j.tibs.2023.10.002 (Source: Trends in Biochemical Sciences)
Source: Trends in Biochemical Sciences - November 10, 2023 Category: Biochemistry Authors: Priya R Banerjee Alex S Holehouse Richard Kriwacki Paul Robustelli Hao Jiang Alexander I Sobolevsky Jennifer M Hurley Joshua T Mendell Source Type: research
Type III CRISPR-Cas: beyond the Cas10 effector complex
Trends Biochem Sci. 2023 Nov 8:S0968-0004(23)00261-X. doi: 10.1016/j.tibs.2023.10.006. Online ahead of print.ABSTRACTType III CRISPR-Cas loci encode some of the most abundant, yet complex, immune systems of prokaryotes. They are composed of a Cas10 complex that uses an RNA guide to recognize transcripts from bacteriophage and plasmid invaders. Target recognition triggers three activities within this complex: ssDNA degradation, synthesis of cyclic oligoadenylates (cOA) that act as second messengers to activate CARF-domain effectors, and cleavage of target RNA. This review covers recent research in type III CRISPR-Cas system...
Source: Trends in Biochemical Sciences - November 10, 2023 Category: Biochemistry Authors: Gianna Stella Luciano Marraffini Source Type: research
UFMylation: a ubiquitin-like modification
Trends Biochem Sci. 2023 Nov 7:S0968-0004(23)00259-1. doi: 10.1016/j.tibs.2023.10.004. Online ahead of print.ABSTRACTPost-translational modifications (PTMs) add a major degree of complexity to the proteome and are essential controllers of protein homeostasis. Amongst the hundreds of PTMs identified, ubiquitin and ubiquitin-like (UBL) modifications are recognized as key regulators of cellular processes through their ability to affect protein-protein interactions, protein stability, and thus the functions of their protein targets. Here, we focus on the most recently identified UBL, ubiquitin-fold modifier 1 (UFM1), and the m...
Source: Trends in Biochemical Sciences - November 9, 2023 Category: Biochemistry Authors: Xingchen Zhou Sayyed J Mahdizadeh Matthieu Le Gallo Leif A Eriksson Eric Chevet Elodie Lafont Source Type: research
UFMylation: a ubiquitin-like modification
Trends Biochem Sci. 2023 Nov 7:S0968-0004(23)00259-1. doi: 10.1016/j.tibs.2023.10.004. Online ahead of print.ABSTRACTPost-translational modifications (PTMs) add a major degree of complexity to the proteome and are essential controllers of protein homeostasis. Amongst the hundreds of PTMs identified, ubiquitin and ubiquitin-like (UBL) modifications are recognized as key regulators of cellular processes through their ability to affect protein-protein interactions, protein stability, and thus the functions of their protein targets. Here, we focus on the most recently identified UBL, ubiquitin-fold modifier 1 (UFM1), and the m...
Source: Trends in Biochemical Sciences - November 9, 2023 Category: Biochemistry Authors: Xingchen Zhou Sayyed J Mahdizadeh Matthieu Le Gallo Leif A Eriksson Eric Chevet Elodie Lafont Source Type: research
Cryo-EM structures of functional and pathological amyloid ribonucleoprotein assemblies
Trends Biochem Sci. 2023 Nov 3:S0968-0004(23)00260-8. doi: 10.1016/j.tibs.2023.10.005. Online ahead of print.ABSTRACTAmyloids are implicated in neurodegenerative and systemic diseases, yet they serve important functional roles in numerous organisms. Heterogeneous nuclear ribonucleoproteins (hnRNPs) represent a large family of RNA-binding proteins (RBPs) that control central events of RNA biogenesis in normal and diseased cellular conditions. Many of these proteins contain prion-like sequences of low complexity, which not only assemble into functional fibrils in response to cellular cues but can also lead to disease when mi...
Source: Trends in Biochemical Sciences - November 5, 2023 Category: Biochemistry Authors: Javier Garcia-Pardo Salvador Ventura Source Type: research
Cryo-EM structures of functional and pathological amyloid ribonucleoprotein assemblies
Trends Biochem Sci. 2023 Nov 3:S0968-0004(23)00260-8. doi: 10.1016/j.tibs.2023.10.005. Online ahead of print.ABSTRACTAmyloids are implicated in neurodegenerative and systemic diseases, yet they serve important functional roles in numerous organisms. Heterogeneous nuclear ribonucleoproteins (hnRNPs) represent a large family of RNA-binding proteins (RBPs) that control central events of RNA biogenesis in normal and diseased cellular conditions. Many of these proteins contain prion-like sequences of low complexity, which not only assemble into functional fibrils in response to cellular cues but can also lead to disease when mi...
Source: Trends in Biochemical Sciences - November 5, 2023 Category: Biochemistry Authors: Javier Garcia-Pardo Salvador Ventura Source Type: research
Cryo-EM structures of functional and pathological amyloid ribonucleoprotein assemblies
Trends Biochem Sci. 2023 Nov 3:S0968-0004(23)00260-8. doi: 10.1016/j.tibs.2023.10.005. Online ahead of print.ABSTRACTAmyloids are implicated in neurodegenerative and systemic diseases, yet they serve important functional roles in numerous organisms. Heterogeneous nuclear ribonucleoproteins (hnRNPs) represent a large family of RNA-binding proteins (RBPs) that control central events of RNA biogenesis in normal and diseased cellular conditions. Many of these proteins contain prion-like sequences of low complexity, which not only assemble into functional fibrils in response to cellular cues but can also lead to disease when mi...
Source: Trends in Biochemical Sciences - November 5, 2023 Category: Biochemistry Authors: Javier Garcia-Pardo Salvador Ventura Source Type: research
Cryo-EM structures of functional and pathological amyloid ribonucleoprotein assemblies
Trends Biochem Sci. 2023 Nov 3:S0968-0004(23)00260-8. doi: 10.1016/j.tibs.2023.10.005. Online ahead of print.ABSTRACTAmyloids are implicated in neurodegenerative and systemic diseases, yet they serve important functional roles in numerous organisms. Heterogeneous nuclear ribonucleoproteins (hnRNPs) represent a large family of RNA-binding proteins (RBPs) that control central events of RNA biogenesis in normal and diseased cellular conditions. Many of these proteins contain prion-like sequences of low complexity, which not only assemble into functional fibrils in response to cellular cues but can also lead to disease when mi...
Source: Trends in Biochemical Sciences - November 5, 2023 Category: Biochemistry Authors: Javier Garcia-Pardo Salvador Ventura Source Type: research
Helicases clear hurdles during plant defense protein translation
Trends Biochem Sci. 2023 Nov 1:S0968-0004(23)00276-1. doi: 10.1016/j.tibs.2023.10.007. Online ahead of print.ABSTRACTPlants undergo translational reprogramming when they are under attack by pathogens. Xiang et al. recently revealed that plant helicases induced by pathogen recognition unwind RNA hairpins upstream of the main open reading frames (mORFs), thus allowing ribosomes to bypass the upstream ORFs (uORFs) and translate downstream defense proteins, a mechanism that is also found in mammals.PMID:37923611 | DOI:10.1016/j.tibs.2023.10.007 (Source: Trends in Biochemical Sciences)
Source: Trends in Biochemical Sciences - November 3, 2023 Category: Biochemistry Authors: Liyuan You Cuilan Shi Daowen Wang Zheng Qing Fu Source Type: research
Mini PROTACs: N-end rule-mediated degradation on the horizon
Trends Biochem Sci. 2023 Nov 2:S0968-0004(23)00256-6. doi: 10.1016/j.tibs.2023.10.001. Online ahead of print.ABSTRACTHeterobifunctional proteolysis-targeting chimeras (PROTACs) offer a promising cancer treatment avenue by efficiently degrading unwanted cellular proteins. A recent study from Zhang et al. demonstrated the successful utilization of the N-end rule in PROTAC design, allowing for a modular degradation rate tailored to the oncogenic driver BCR-ABL.PMID:37923612 | DOI:10.1016/j.tibs.2023.10.001 (Source: Trends in Biochemical Sciences)
Source: Trends in Biochemical Sciences - November 3, 2023 Category: Biochemistry Authors: Yanfen Liu Zhangshun Wang Yong Cang Source Type: research
Cotranslational sorting and processing of newly synthesized proteins in eukaryotes
Trends Biochem Sci. 2023 Nov 1:S0968-0004(23)00258-X. doi: 10.1016/j.tibs.2023.10.003. Online ahead of print.ABSTRACTRibosomes interact with a variety of different protein biogenesis factors that guide newly synthesized proteins to their native 3D shapes and cellular localization. Depending on the type of translated substrate, a distinct set of cotranslational factors must interact with the ribosome in a timely and coordinated manner to ensure proper protein biogenesis. While cytonuclear proteins require cotranslational maturation and folding factors, secretory proteins must be maintained in an unfolded state and processed...
Source: Trends in Biochemical Sciences - November 2, 2023 Category: Biochemistry Authors: Martin Gamerdinger Elke Deuerling Source Type: research
