Intrinsically disordered regions are poised to act as sensors of cellular chemistry
Trends Biochem Sci. 2023 Aug 30:S0968-0004(23)00204-9. doi: 10.1016/j.tibs.2023.08.001. Online ahead of print.ABSTRACTIntrinsically disordered proteins and protein regions (IDRs) are abundant in eukaryotic proteomes and play a wide variety of essential roles. Instead of folding into a stable structure, IDRs exist in an ensemble of interconverting conformations whose structure is biased by sequence-dependent interactions. The absence of a stable 3D structure, combined with high solvent accessibility, means that IDR conformational biases are inherently sensitive to changes in their environment. Here, we argue that IDRs are i...
Source: Trends in Biochemical Sciences - September 1, 2023 Category: Biochemistry Authors: David Moses Garrett M Ginell Alex S Holehouse Shahar Sukenik Source Type: research
Membrane homeostasis beyond fluidity: control of membrane compressibility
Trends Biochem Sci. 2023 Aug 29:S0968-0004(23)00207-4. doi: 10.1016/j.tibs.2023.08.004. Online ahead of print.ABSTRACTBiomembranes are complex materials composed of lipids and proteins that compartmentalize biochemistry. They are actively remodeled in response to physical and metabolic cues, as well as during cell differentiation and stress. The concept of homeoviscous adaptation has become a textbook example of membrane responsiveness. Here, we discuss limitations and common misconceptions revolving around it. By highlighting key moments in the life cycle of a transmembrane protein, we illustrate that membrane thickness a...
Source: Trends in Biochemical Sciences - August 31, 2023 Category: Biochemistry Authors: Mike F Renne Robert Ernst Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Trends Biochem Sci. 2023 Aug 16:S0968-0004(23)00176-7. doi: 10.1016/j.tibs.2023.07.010. Online ahead of print.ABSTRACTCENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromere-associated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognitio...
Source: Trends in Biochemical Sciences - August 18, 2023 Category: Biochemistry Authors: Seyit Kale Ramachandran Boopathi Edwige Belotti Imtiaz Nisar Lone Mohamed Graies Maria Schr öder Maria Petrova Christophe Papin Jan Bednar Iva Ugrinova Ali Hamiche Stefan Dimitrov Source Type: research
Models for human telomere C-strand fill-in by CST-Pol α-primase
Trends Biochem Sci. 2023 Aug 15:S0968-0004(23)00174-3. doi: 10.1016/j.tibs.2023.07.008. Online ahead of print.ABSTRACTTelomere maintenance is essential for the genome integrity of eukaryotes, and this function is underpinned by the two-step telomeric DNA synthesis process: telomere G-overhang extension by telomerase and complementary strand fill-in by DNA polymerase alpha-primase (polα-primase). Compared to the telomerase step, the telomere C-strand fill-in mechanism is less understood. Recent studies have provided new insights into how telomeric single-stranded DNA-binding protein CTC1-STN1-TEN1 (CST) and polα-primase c...
Source: Trends in Biochemical Sciences - August 16, 2023 Category: Biochemistry Authors: Qixiang He Ci Ji Lim Source Type: research
Models for human telomere C-strand fill-in by CST-Pol α-primase
Trends Biochem Sci. 2023 Aug 15:S0968-0004(23)00174-3. doi: 10.1016/j.tibs.2023.07.008. Online ahead of print.ABSTRACTTelomere maintenance is essential for the genome integrity of eukaryotes, and this function is underpinned by the two-step telomeric DNA synthesis process: telomere G-overhang extension by telomerase and complementary strand fill-in by DNA polymerase alpha-primase (polα-primase). Compared to the telomerase step, the telomere C-strand fill-in mechanism is less understood. Recent studies have provided new insights into how telomeric single-stranded DNA-binding protein CTC1-STN1-TEN1 (CST) and polα-primase c...
Source: Trends in Biochemical Sciences - August 16, 2023 Category: Biochemistry Authors: Qixiang He Ci Ji Lim Source Type: research
Models for human telomere C-strand fill-in by CST-Pol α-primase
Trends Biochem Sci. 2023 Aug 15:S0968-0004(23)00174-3. doi: 10.1016/j.tibs.2023.07.008. Online ahead of print.ABSTRACTTelomere maintenance is essential for the genome integrity of eukaryotes, and this function is underpinned by the two-step telomeric DNA synthesis process: telomere G-overhang extension by telomerase and complementary strand fill-in by DNA polymerase alpha-primase (polα-primase). Compared to the telomerase step, the telomere C-strand fill-in mechanism is less understood. Recent studies have provided new insights into how telomeric single-stranded DNA-binding protein CTC1-STN1-TEN1 (CST) and polα-primase c...
Source: Trends in Biochemical Sciences - August 16, 2023 Category: Biochemistry Authors: Qixiang He Ci Ji Lim Source Type: research
