The optimal standard protocols for whole-genome sequencing of antibiotic-resistant pathogenic bacteria using third-generation sequencing platforms
ConclusionDNA extraction, sequencing platform, and assembly methods can all have an impact on the results of bacterial whole-genome sequencing. Our findings could be a practical protocol for researchers who use third-generation sequencing to perform bacterial whole-genome sequencing by consistently resolving small plasmids carrying antibiotic-resistance genes. (Source: Molecular and Cellular Toxicology)
Source: Molecular and Cellular Toxicology - August 13, 2021 Category: Cytology Source Type: research
Biochemical and physiological responses of the water flea Moina macrocopa to microplastics: a multigenerational study
ConclusionOur results suggest that the detrimental effects of prolonged exposure to polystyrene microbeads would be concentration dependent and harmful for the maintenance of water flea populations by induction of oxidative stress and failure in antioxidant defense. (Source: Molecular and Cellular Toxicology)
Source: Molecular and Cellular Toxicology - August 11, 2021 Category: Cytology Source Type: research
Down-regulation of PTTG1 suppresses PDGF-BB-induced proliferation, migration and extracellular matrix production of airway smooth muscle cells (ASMCs) by regulating PI3K/AKT/mTOR signaling pathway
ConclusionsPTTG1 could serve as a promising molecular target for the treatment of asthma. (Source: Molecular and Cellular Toxicology)
Source: Molecular and Cellular Toxicology - July 27, 2021 Category: Cytology Source Type: research
Naringenin alleviates bone cancer pain in rats via down-regulating spinal P2X7R /PI3K/AKT signaling: involving suppression in spinal inflammation
ConclusionNAR alleviated BCP through inhibiting inflammatory cytokines and down-regulating P2X7R/PI3K/AKT signaling in the spinal cord of rats. These findings revealed that NAR, as an effective agent against BCP, may provide an effective approach in the management of bone cancer patients.Graphic abstract (Source: Molecular and Cellular Toxicology)
Source: Molecular and Cellular Toxicology - July 19, 2021 Category: Cytology Source Type: research
Gold nanoparticles: uptake in human mast cells and effect on cell viability, inflammatory mediators, and proliferation
ConclusionInternalization of AuNPs into HMC-1 cells was demonstrated in an in vitro model, without showing cytotoxic effects or induction of pro-inflammatory mediators at any concentration tested. (Source: Molecular and Cellular Toxicology)
Source: Molecular and Cellular Toxicology - July 14, 2021 Category: Cytology Source Type: research
