Endometrial microRNAs and their aberrant expression patterns
AbstractMicroRNAs (miRNAs) are a class of small noncoding RNAs that regulate gene expression. They play fundamental roles in several biological processes, including cell differentiation and proliferation, embryo development, organ development, and organ metabolism. Besides regulating the physiological processes, miRNAs regulate various pathological conditions such as tumors, metastases, metabolic diseases, and osteoporosis. Although several studies have been performed on miRNAs, only few studies have described the miRNA expression and functions in human repr oductive tract tissues. During menstruation, the human endometr...
Source: Medical Molecular Morphology - April 28, 2020 Category: Molecular Biology Source Type: research
Chronic stress-induced elevation of IL-1 β in the saliva and submandibular glands of mice
We examined the effect of chronic stress on three major inflammatory cytokines Interleukin (IL)-1 β, IL-6, and tumor necrosis factor (TNF)-α in the saliva and salivary glands of chronically stressed mice. Six-week-old C57BL/6 J mice were randomly divided into a control and a stress group. The mice in stress group were exposed to 4 h of stress daily for 10 days and subsequently saliva, as we ll as the submandibular glands, were collected from both groups. The expression levels of cytokines in the saliva were examined by enzyme-linked immunosorbent assay. The submandibular glands were subjected to histopathological and ...
Source: Medical Molecular Morphology - April 5, 2020 Category: Molecular Biology Source Type: research
Hesperetin alleviated glucocorticoid-induced inhibition of osteogenic differentiation of BMSCs through regulating the ERK signaling pathway
In conclusion, our study demonstrated that hesperetin alleviated glucocorticoid-induced inhibition of osteogenic differentiation through ERK signal pathway in BMSCs. It may be a potential therapeutic agent for protecting against glucocorticoid-induced osteoporosis. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - April 5, 2020 Category: Molecular Biology Source Type: research
Expression of HIF-2a in clear-cell renal cell carcinoma independently predicts overall survival
AbstractThe purpose of this study is to evaluate the expression and the prognostic role of main factors, involved in the hypoxia pathway, in patients with clear-cell renal cell carcinoma (ccRCC). Immunohistochemical expression of Hypoxia inducible factors (HIF) HIF-1a, HIF-2a, prolyl hydroxylases PHD1, PHD2, PHD3, and factor inhibiting HIF (FIH) was assessed on a tissue microarray, containing tumour and corresponding normal kidney tissue from 66 patients underwent surgery for ccRCC. Expression levels were evaluated in relation to T stage, Fuhrman grade, cancer-specific, and overall survival (OS). Cytoplasmatic expression o...
Source: Medical Molecular Morphology - March 25, 2020 Category: Molecular Biology Source Type: research
Biochemical and morphological characterization of SEPT1 in mouse brain
In this study, we produced a specific antibody against mouse SEPT1 and carried out biochemical and morphological characterization of SEPT1. When the expressio n profile of SEPT1 during mouse brain development was analyzed by western blotting, we found that SEPT1 expression began to increase after birth and the increase continued until postnatal day 22. Subcellular fractionation of mouse brain and subsequent western blot analysis revealed the distribution of SEPT1 in synaptic fractions. Immunofluorescent analyses showed the localization of SEPT1 at synapses in primary cultured mouse hippocampal neurons. We also found the di...
Source: Medical Molecular Morphology - March 6, 2020 Category: Molecular Biology Source Type: research
ATM kinase regulates tumor immunoreactions in lymphocyte-predominant breast cancer through modulation of NKG2D ligand and TNF cytokines on tumor cells
AbstractTo explore impact of Ataxia telangiectasia mutated (ATM) kinase on immunoreactions in lymphocyte-predominant breast cancer (LPBC), particularly its role in triple negative breast cancer (TNBC), 194 cases of LPBC were identified with pertinent clinical information retrieved. The expressions of ATM, activated ATM (P-ATM), Fas ligand (FASL), tumor necrosis factor-related apoptosis-induced ligand (TRAIL), major histocompatibility complex class I chain-related protein A (MICA), CD8, and Forkhead box P3 (FOXP3) were assessed by immunohistochemically. We found that ATM expressed on tumor cells was correlated with upregula...
Source: Medical Molecular Morphology - February 16, 2020 Category: Molecular Biology Source Type: research
Sequential pattern of sublayer formation in the paleocortex and neocortex
AbstractThe piriform cortex (paleocortex) is the olfactory cortex or the primary cortex for the sense of smell. It receives the olfactory input from the mitral and tufted cells of the olfactory bulb and is involved in the processing of information pertaining to odors. The piriform cortex and the adjoining neocortex have different cytoarchitectures; while the former has a three-layered structure, the latter has a six-layered structure. The regulatory mechanisms underlying the building of the six-layered neocortex are well established; in contrast, less is known about of the regulatory mechanisms responsible for structure fo...
Source: Medical Molecular Morphology - January 29, 2020 Category: Molecular Biology Source Type: research
Ultrastructural changes in the choriocapillaris of N -methyl- N -nitrosourea-induced retinal degeneration in C57BL/6 mice
AbstractN-methyl-N-nitrosourea (MNU) is known to cause apoptosis of photoreceptor cells and changes in retinal pigment epithelium (RPE). However, the changes in choriocapillaris, which nourishes photoreceptor cells by diffusing tissue fluid through RPE, have not been reported in detail. Therefore, we studied the ultrastructural transformation in and around the choriocapillaris to characterize the interdependence between choriocapillaris and surrounding tissue components in a mouse model. Seven-week-old male C57BL/6 mice were given a single intraperitoneal injection of MNU (60 mg/kg of body weight). Perfusion-fixed eyebal...
Source: Medical Molecular Morphology - January 29, 2020 Category: Molecular Biology Source Type: research
The expression of MYC is strongly dependent on the circular PVT1 expression in pure Gleason pattern 4 of prostatic cancer
AbstractPVT1 is a long-noncoding RNA and is highly expressed in various cancers including prostate cancers with stabilizing MYC protein. To characterize the objective biological features of the different morphological components such as Gleason patterns (GP) in prostate cancer, biopsy specimens containing only single pure GP (GP3, GP4, GP5) are used to analyze the relationship betweenPVT1 expression and MYC protein expression. The expressions ofPVT1 andMYC were analyzed by quantitative PCR and the labeling index (LI) of MYC protein by immunohistochemical staining.PVT1,MYC, and MYC protein were highly expressed in GP 4, and...
Source: Medical Molecular Morphology - January 12, 2020 Category: Molecular Biology Source Type: research
Histological evaluation of the acetabular labrum after bipolar hip hemiarthroplasty: a case report
We report herein the microstructure of the acetabular labrum obtained from a patient with stem loosening but without bipolar cup migration who had undergone hemiarthroplasty for femoral neck fracture 18 years ago. We used light and scanning electron microscopy to investigate the influence of bipolar cup on acetabular labrum in vivo. Deparaffinized blocks were treated with 2 N NaOH to digest the cell matrix, allowing the collagen fibers, constituting the acetabular labrum, to be observed under sc anning electron microscopy. Although chondrocyte atrophy was seen, the basic structure was not different from the normal tissu...
Source: Medical Molecular Morphology - January 12, 2020 Category: Molecular Biology Source Type: research
Desmoplastic small round cell tumor showing solid proliferation with limited desmoplasia and confusing immunohistochemical findings: an autopsy report
AbstractWe herein report a variant case of desmoplastic small round cell tumor (DSRCT) showing limited desmoplasia and confusing immunohistochemical findings. A 26-year-old male was referred for multiple abdominal masses. Laparoscopic biopsy showed only the solid proliferation of small round cells, and he was initially diagnosed with small cell carcinoma. At autopsy, the tumor spread diffusely throughout the abdominal and pelvic cavities. Although the tumor was composed of a predominantly solid pattern of small round cells, multiple samples revealed a fibrous stroma in limited areas only. While immunohistochemistry showed ...
Source: Medical Molecular Morphology - January 5, 2020 Category: Molecular Biology Source Type: research
Clinicopathologic analysis of gastric mucosa-associated lymphoid tissue lymphoma with or without c-Met expression
AbstractExtranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT) lymphoma of the stomach is mainly associated withHelicobacter pylori infection, andH. pylori eradication therapy is often effective. However, 20 –30% of the cases of MALT lymphoma are resistant to the eradication therapy, and translocation of theAPI2-MALT1 gene is often found in these cases. Most cases without translocation ofAPI2-MALT1 are localized to the stomach, whereas some cases with this translocation are a more advanced stage of MALT lymphoma that spreads to other organs. The c-Met receptor is a prognostic factor involved in inf...
Source: Medical Molecular Morphology - January 1, 2020 Category: Molecular Biology Source Type: research
Peroxisome proliferator-activated receptor ɑ (PPARɑ)–cytochrome P450 epoxygenases-soluble epoxide hydrolase axis in ER + PR + HER2− breast cancer
AbstractFibrates belong to a group of ligands of peroxisome proliferator-activated receptor alpha (PPAR α), which play a role in the regulation of CYP epoxygenases and soluble epoxide hydrolase (sEH), key enzymes in the metabolism of biologically highly active epoxyeicosatrienoic acids (EETs). We demonstrated that low doses of fibrates stimulate proliferation of the MCF7 cell line, while high doses s uppress it. The increase in cell proliferation was accompanied by an increase in CYP epoxygenases and decrease in sEH levels. The overall level of PPARα remained same after low-dose fibrate stimulation; however, there was a ...
Source: Medical Molecular Morphology - December 9, 2019 Category: Molecular Biology Source Type: research
Scanning electoronmicroscopical analysis of the loose bodies of synovial chondoromatosis in temporomandibular joint
AbstractThe aim of this study is to reveal the morphological property about the loose bodies (LBs) of temporomandibular joint (TMJ) by scanning electron microscope (SEM). We obtained specimens from two female cases of released loose body by surgical operation. These specimens were fixed by soaking in a mixture of 5% glutaraldehyde or 4% formaldehyde for one week. They were cut into half pieces. These specimens were observed at an accelerating voltage of 3 kV under a SEM (JSM-5500, JEOL, Tokyo). In the electron microscopic findings, it seems to be separated into two different parts as inside part and outside part. On the ...
Source: Medical Molecular Morphology - December 8, 2019 Category: Molecular Biology Source Type: research
Interleukin-24 is a novel diagnostic biomarker for the severity of acute kidney injury
In this study, we monitored interleukin (IL)-24 expressed in kidneys with severe AKI that progresses to atrophic kidney in a mouse model of ischemia –reperfusion injury (IRI). Therefore, we evaluated IL-24 as a potential biomarker not only for early diagnosis of AKI, but also for predicting progression to chronic kidney disease (CKD). Serum IL-24 was detected earlier than the elevation of serum creatinine levels and urinary IL-24 was detected as early as neutrophil gelatinase associated lipocalin (NGAL) in severe AKI (60 min of IRI). In addition, serum and urine IL-24 levels tended to increase in relation to ischemia du...
Source: Medical Molecular Morphology - December 3, 2019 Category: Molecular Biology Source Type: research
