Mucinous adenocarcinoma, gastric type of the uterine cervix: clinical features and HER2 amplification
The objectives of this study were to identify the clinicopathological characteristics of GAS and to evaluate HER2 expression in GAS. We retrospectively reviewed 322 cervical cancer cases diagnosed at the Kyoto University Hospital from 2010 to 2016. The incidence, clinical factors including age, stage, and lymph node status, tumor markers, immunoreactive expression of MUC6, HIK1083, and HER2, andHER2 amplification were evaluated. Of the 322 cases of cervical cancer, 13 cases of the adenocarcinoma cases were diagnosed as GAS. Watery discharge, lower abdominal pain, CA19-9 elevation, and lymph node metastasis were frequently ...
Source: Medical Molecular Morphology - July 10, 2018 Category: Molecular Biology Source Type: research
Positive correlation between the density of macrophages and T-cells in undifferentiated sarcoma
In this study, we evaluated tumor-associated macrophages (TAMs) and CD8-positive cytotoxic T lymphocytes (CTLs) in 28 cases of US. Iba1, CD163, and CD204 were used as markers for TAMs. The density of CTLs was positively correlated with the density of TAMs. However, a negative correlation was seen between the density of CTLs and the percentage of CD204-positive TAMs. We found no significant association between the density of Iba1-/CD204-/CD8-positive cells and clinicopathological factors. No significant correlation between immune cell infiltration and clinical outcome was observed. Although we found no significant associati...
Source: Medical Molecular Morphology - July 6, 2018 Category: Molecular Biology Source Type: research
Combination of APD668, a G protein-coupled receptor 119 agonist with linagliptin, a DPPIV inhibitor, prevents progression of steatohepatitis in a murine model of non-alcoholic steatohepatitis with diabetes
In this study, we explored the effect of APD668, a GPR119 agonist alone or in combination with linagliptin, a DPPIV inhibitor, on the progression of steatohepatitis in a murine model of NASH with diabetes. A novel NASH model with diabetes was generated by administration of streptozotocin injection to neonatal C57BL/6 mice (2 –3 days old) combined with a high-fat diet feeding from the age of 4 weeks. The plasma biochemical parameters, oxidative stress, inflammation and histopathological changes were assessed. APD668 alone showed reduction in plasma glucose (− 39%,P < 0.05) and triglyceride level (− 26%)...
Source: Medical Molecular Morphology - June 29, 2018 Category: Molecular Biology Source Type: research
Immunoreactivity patterns of tight junction proteins are useful for differential diagnosis of human salivary gland tumors
In this study, we examined the immunoreactivity of claudin (CLDN)-1, -4, and -7, and JAM-A in salivary gland tumors (SGTs) by histological types and cell types to estimate their usefulness as differential diagnostic markers. Immunoreactivity of CLDN1 was higher in ductal epithelium cells of SGTs than in non-tumor tissues. Conversely, immunoreactivity of CLDN1 was significantly decreased in basal/myoepithelium cells of SGTs compared with that in non-tumor tissues. There was no significant difference between the immunoreactivity of CLDN1 in benign tumors and that in malignant tumors. Immunoreactivity of CLDN4, CLDN7, and JAM...
Source: Medical Molecular Morphology - June 28, 2018 Category: Molecular Biology Source Type: research
Physical exercise alters hepatic morphology of low-density lipoprotein receptor knockout ovariectomized mice
AbstractTo explore the effects of physical exercise on the liver of animals in menopause, we analyzed the histomorphometric parameters of the hepatic tissue in ovariectomized and dyslipidemic female mice. The animals were distributed in six groups (n = 5): sedentary control (SC), sedentary ovariectomized control (SOC), trained ovariectomized control (TOC), sedentary LDL knockout (LDL-S), sedentary ovariectomized LDL knockout (LDL-SO), and trained ovariectomized LDL knockout (LDL-TO). At the end of the experiment, the liver and the visceral adipose tissue (VAT) of animals were removed for morphometric and stereological ...
Source: Medical Molecular Morphology - June 22, 2018 Category: Molecular Biology Source Type: research
Overexpression of folate receptor alpha is an independent prognostic factor for outcomes of pancreatic cancer patients
AbstractPancreatic cancer has a poor prognosis; hence, novel prognostic markers and effective therapeutic targets should be identified. We aimed to evaluate folate receptor alpha (FR- α) expression in pancreatic cancer and examine its association with clinicopathological features. We utilized tissue samples from 100 primary pancreatic cancer patients who underwent surgery. FR-α was expressed in 37 of 100 cases (37%). The FR-α-positive group (median, 18.8 months) had a signific antly poorer prognosis than the FR-α-negative group [median 21.3 months; HR 1.89 (1.12–3.12);P = 0.017]. These groups were not significant...
Source: Medical Molecular Morphology - June 20, 2018 Category: Molecular Biology Source Type: research
Validation and application of a novel APC antibody in western blotting, immunoprecipitation, and immunohistochemistry
In this study, we generated an antibody against the c-terminal domain of human APC, designated APC-C antibody, and evaluated its specificity and application in various immunological meth ods. Our data indicate that this novel APC-C antibody is a specific and versatile antibody that can be used in western blotting, immunoprecipitation, immunocytochemistry, and immunohistochemistry. Widespread use of this APC antibody will help enhance our understanding of APC’s function in both nor mal and cancer cell biology. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - June 19, 2018 Category: Molecular Biology Source Type: research
