Utility of human epidermal growth factor 2 heterogeneity as a prognostic factor in triple-negative breast cancer
AbstractIn some cases of human epidermal growth factor 2 (HER2)-negative breast cancer, including triple-negative breast cancer, HER2 expression is sporadically and strongly upregulated, a condition known asHER2 heterogeneity. We investigated the clinicopathological features of patients withHER2 heterogeneity in triple-negative breast cancers treated with neoadjuvant chemotherapy. Thirty-nine patients with triple-negative breast cancer who had undergone preoperative chemotherapy participated in this study. To assess forHER2 heterogeneity, we used dual in situ hybridization slides. We evaluated the association betweenHER2 h...
Source: Medical Molecular Morphology - April 15, 2024 Category: Molecular Biology Source Type: research
P4HA2 involved in SLUG-associated EMT predicts poor prognosis of patients with KRAS-positive colorectal cancer
In conclusions, our data highlight that both SLUG and P4HA2 expression may serve as potentially important poor prognostic biomarkers in CRC and targeting these molecules may be providing a novel therapeutic strategy. In KRAS mutation group, high P4HA2 expression is the only independent prognostic factor for tumor recurrence, so it can be suggested to be a novel target for therapy. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - March 24, 2024 Category: Molecular Biology Source Type: research
Alpha-fetoprotein producing endometrioid carcinoma arising in an adenomyoma of the uterus
In conclusion, this case underscores the diagnostic challenges posed by AFP-ECs arising from adenomyomas and emphasizes the need for refining the nomenclature and classification of AFP-producing neoplasms within the endometrium. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - March 11, 2024 Category: Molecular Biology Source Type: research
Clinical and molecular characteristics of Jordanian oropharyngeal cancer patients according to P16 expression: a retrospective study and a report of a novel biomarker
AbstractThe purpose of this study was to assess the clinicopathological features of oropharyngeal cancer patients in Jordan based on their HPV status. Sixty-nine biopsies from two hospitals were included. Tissue microarrays were prepared from formalin-fixed paraffin-embedded (FFPE) specimens and  stained with antibodies for CDKN2A/P16, EGFR, PI3K, PTEN, AKT, pS473AKT, PS2mTOR, and TIMAP. The cohort was divided according to P16 expression. Chi-square test and survival analyses were employed to evaluate the variations among the study variables and determine the prognostic factors, respective ly. P16 expression was found in ...
Source: Medical Molecular Morphology - March 9, 2024 Category: Molecular Biology Source Type: research
Genetic and histological analysis intraplacental choriocarcinoma: a case report
We report on single case of intraplacental choriocarcinoma (IC) coexisting with feto-maternal hemorrhage from our hospital, a rare malignant tumor that occurs in the chorionic villous trophoblast. To investigate genetic and epigenetic changes to the carcinogenesis of IC, we employed cancer gene panel analysis and whole methylation analysis from a recent case of IC. By Short Tandem Repeats analysis, we confirmed that the tumor of present IC was derived from concurrent normal chorionic villous trophoblast cells. No mutation was found in 145 cancer-related genes. Meanwhile, amplification inMDM2 gene was observed. Furthermore,...
Source: Medical Molecular Morphology - February 29, 2024 Category: Molecular Biology Source Type: research
Morphological analysis for two types of viral particles in vacuoles of SARS-CoV-2-infected cells
In this study, we analyzed the morphological structure of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in human cells. We identified the two types of viral particles present within the vacuoles of infected cells. Using transmission electron microscopy, we observed that SARS-CoV-2 particles exhibited both low- and high-electron-density structures, which was further confirmed through three-dimensional reconstruction using electron tomography. The budding of these particles was exclusively observed within these vacuoles. Intriguingly, viral particles with low-electron-density structures were confined to vacuol...
Source: Medical Molecular Morphology - February 23, 2024 Category: Molecular Biology Source Type: research
Suppressed distribution of protein A on the surface of Staphylococcus aureus as a morphological characteristic of erythromycin-resistant strain
AbstractTo identify a new morphological phenotype of erythromycin (EM)-resistantStaphylococcus aureus (S. aureus) were isolated in vitro from EM-sensitive parent strain, and the distribution of staphylococcus specific protein A (SpA) on the surface of these strains was examined morphologically by using applied immunoelectron microscopy. The isolated EM-resistant strains had thickened cell walls, and the distribution of SpA on the surfaces of these strains was demonstrated to be lower than that of the parent strain. The SpA suppression was confirmed by enzyme-linked immunosorbent assay (ELISA) using fixed EM-resistant cells...
Source: Medical Molecular Morphology - February 22, 2024 Category: Molecular Biology Source Type: research
Characteristics of peripheral blood mononuclear cells and potential related molecular mechanisms in patients with autoimmune hepatitis: a single-cell RNA sequencing analysis
This study was designed to analyze peripheral blood mononuclear cell (PBMC) characteristics in AIH through single-cell RNA sequencing (scRNA-seq) and explore potential AIH-related molecular mechanisms. We generated 3690 and 3511 single-cell transcriptomes of PBMCs pooled from 4 healthy controls (HCs) and 4 AIH patients, respectively, by scRNA-seq. These pooled PBMC transcriptomes were used for cell cluster identification and differentially expressed gene (DEG) identification. GO functional enrichment analysis was performed on the DEGs to determine the most active AIH immune cell biological functions. Although the PCA-based...
Source: Medical Molecular Morphology - February 10, 2024 Category: Molecular Biology Source Type: research
IL-32 production from lung adenocarcinoma cells is potentially involved in immunosuppressive microenvironment
In conclusion, IL-32 potentially induced by inflammatory conditions and anticancer therapy and contribute to immune escape of cancer cells via development the immunosuppressive microenvironment. IL-32 might be a target molecule for anti-cancer therapy. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - February 6, 2024 Category: Molecular Biology Source Type: research
Usage of nivolumab and ipilimumab for recurrent or advanced malignant vaginal melanoma: a two-case series
AbstractImmune checkpoint inhibitors help treat malignant melanoma, but show limited use in treating malignant vaginal melanoma, an aggressive, rare gynecological malignancy. We identified two patients treated with ipilimumab and nivolumab for vaginal melanoma; both were immunonegative for programmed cell death-ligand 1 and wild-typeBRAF. Case 1, a 56-year-old female who underwent radical surgery for stage 1 malignant vaginal melanoma, experienced recurrence 15 Â months postoperatively. She briefly responded to ipilimumab and nivolumab combination therapy before showing disease progression. Tumor shrinkage occurred with ni...
Source: Medical Molecular Morphology - January 30, 2024 Category: Molecular Biology Source Type: research
Transmission electron microscopic study of the surface layer of surgical resected disc specimens in human temporomandibular joint
In this study, we investigated specific and characteristic findings of the surface layer of surgical resected disc specimens in human temporomandibular joint osteoarthritis cases by transmission electron microscopy (TEM).Specimens were surgically removed from the TMJ of 5 cases (4 female patients: 5 cases) clinically osteoarthritis. Following findings were observed by TEM. Images were photographed on a JEM1400-Flash Electron microscope (JEOL, Japan) equipped with an EM-14661FLASH high-sensitivity digital complementary metal –oxide–semiconductor camera.Following findings were observed by TEM.The surface is covered with ...
Source: Medical Molecular Morphology - December 9, 2023 Category: Molecular Biology Source Type: research
Comment to a patient with SLC40A1-HC successfully treated using red blood cell apheresis
(Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - November 27, 2023 Category: Molecular Biology Source Type: research
Ultrastructural changes of vascular smooth muscle cells and resistance to vasospasm treatment in femoral arteries of an arteriosclerotic rat model
This study revealed the development of vasospasm in the femoral arteries of the arteriosclerotic rats and, a conventional vasodilator did not releas e the vasospasm. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - November 22, 2023 Category: Molecular Biology Source Type: research
Overexpression of SerpinB9 in non-seminomatous germ cell tumors
AbstractSerpinb9 is an inhibitor of granzyme B and is potentially involved in the immune escape of tumor cells. In the present study, bioinformatics analysis using open databases suggested that SerpinB9 is overexpressed in testicular embryonal carcinoma. Immunohistological analysis was performed on 28 cases of testicular germ cell tumors to investigate the relationship between SerpinB9 expression in testicular germ cell tumors and the tumor immune environment. SerpinB9 was significantly upregulated in the non-seminoma group and inversely correlated with the number of tumor-infiltrating CD8-positive cells. In addition, yolk...
Source: Medical Molecular Morphology - November 22, 2023 Category: Molecular Biology Source Type: research
Δ3-tubulin impairs mitotic spindle morphology and increases nuclear size in pancreatic cancer cells
In this study, we investigated the impacts of forced expression of Δ3-tubulin on the structure of spindle bodies and cell division in a pancreatic cancer cell line, PANC-1. Overexpression of HA-tagged Δ3-tubulin impaired the morphology and orientation of spindle bodies during cell division in PANC-1 cells. In particular, spindle bending was most significantly increased. Expression of EGFP-tagged Δ3-tubulin driven by the endogenous promoter of humanTUBA1B also deformed and misoriented spindle bodies. Spindle bending and condensation defects were significantly observed by EGFP- Δ3-tubulin expression. Furthermore, EGFP-Î...
Source: Medical Molecular Morphology - November 6, 2023 Category: Molecular Biology Source Type: research
