The expression of MYC is strongly dependent on the circular PVT1 expression in pure Gleason pattern 4 of prostatic cancer
AbstractPVT1 is a long-noncoding RNA and is highly expressed in various cancers including prostate cancers with stabilizing MYC protein. To characterize the objective biological features of the different morphological components such as Gleason patterns (GP) in prostate cancer, biopsy specimens containing only single pure GP (GP3, GP4, GP5) are used to analyze the relationship betweenPVT1 expression and MYC protein expression. The expressions ofPVT1 andMYC were analyzed by quantitative PCR and the labeling index (LI) of MYC protein by immunohistochemical staining.PVT1,MYC, and MYC protein were highly expressed in GP 4, and...
Source: Medical Molecular Morphology - January 13, 2020 Category: Molecular Biology Source Type: research

Histological evaluation of the acetabular labrum after bipolar hip hemiarthroplasty: a case report
We report herein the microstructure of the acetabular labrum obtained from a patient with stem loosening but without bipolar cup migration who had undergone hemiarthroplasty for femoral neck fracture 18  years ago. We used light and scanning electron microscopy to investigate the influence of bipolar cup on acetabular labrum in vivo. Deparaffinized blocks were treated with 2 N NaOH to digest the cell matrix, allowing the collagen fibers, constituting the acetabular labrum, to be observed under sc anning electron microscopy. Although chondrocyte atrophy was seen, the basic structure was not different from the norm...
Source: Medical Molecular Morphology - January 13, 2020 Category: Molecular Biology Source Type: research

Desmoplastic small round cell tumor showing solid proliferation with limited desmoplasia and confusing immunohistochemical findings: an autopsy report
AbstractWe herein report a variant case of desmoplastic small round cell tumor (DSRCT) showing limited desmoplasia and confusing immunohistochemical findings. A 26-year-old male was referred for multiple abdominal masses. Laparoscopic biopsy showed only the solid proliferation of small round cells, and he was initially diagnosed with small cell carcinoma. At autopsy, the tumor spread diffusely throughout the abdominal and pelvic cavities. Although the tumor was composed of a predominantly solid pattern of small round cells, multiple samples revealed a fibrous stroma in limited areas only. While immunohistochemistry showed ...
Source: Medical Molecular Morphology - January 6, 2020 Category: Molecular Biology Source Type: research

Clinicopathologic analysis of gastric mucosa-associated lymphoid tissue lymphoma with or without c-Met expression
AbstractExtranodal marginal zone lymphoma of mucosa-associated lymphoid tissue (MALT) lymphoma of the stomach is mainly associated withHelicobacter pylori infection, andH. pylori eradication therapy is often effective. However, 20 –30% of the cases of MALT lymphoma are resistant to the eradication therapy, and translocation of theAPI2-MALT1 gene is often found in these cases. Most cases without translocation ofAPI2-MALT1 are localized to the stomach, whereas some cases with this translocation are a more advanced stage of MALT lymphoma that spreads to other organs. The c-Met receptor is a prognostic factor involved in...
Source: Medical Molecular Morphology - January 2, 2020 Category: Molecular Biology Source Type: research

Peroxisome proliferator-activated receptor ɑ (PPARɑ)–cytochrome P450 epoxygenases-soluble epoxide hydrolase axis in ER + PR + HER2− breast cancer
AbstractFibrates belong to a group of ligands of peroxisome proliferator-activated receptor alpha (PPAR α), which play a role in the regulation of CYP epoxygenases and soluble epoxide hydrolase (sEH), key enzymes in the metabolism of biologically highly active epoxyeicosatrienoic acids (EETs). We demonstrated that low doses of fibrates stimulate proliferation of the MCF7 cell line, while high doses s uppress it. The increase in cell proliferation was accompanied by an increase in CYP epoxygenases and decrease in sEH levels. The overall level of PPARα remained same after low-dose fibrate stimulation; however, th...
Source: Medical Molecular Morphology - December 10, 2019 Category: Molecular Biology Source Type: research

Scanning electoronmicroscopical analysis of the loose bodies of synovial chondoromatosis in temporomandibular joint
AbstractThe aim of this study is to reveal the morphological property about the loose bodies (LBs) of temporomandibular joint (TMJ) by scanning electron microscope (SEM). We obtained specimens from two female cases of released loose body by surgical operation. These specimens were fixed by soaking in a mixture of 5% glutaraldehyde or 4% formaldehyde for one week. They were cut into half pieces. These specimens were observed at an accelerating voltage of 3  kV under a SEM (JSM-5500, JEOL, Tokyo). In the electron microscopic findings, it seems to be separated into two different parts as inside part and outside part. On ...
Source: Medical Molecular Morphology - December 9, 2019 Category: Molecular Biology Source Type: research

Interleukin-24 is a novel diagnostic biomarker for the severity of acute kidney injury
In this study, we monitored interleukin (IL)-24 expressed in kidneys with severe AKI that progresses to atrophic kidney in a mouse model of ischemia –reperfusion injury (IRI). Therefore, we evaluated IL-24 as a potential biomarker not only for early diagnosis of AKI, but also for predicting progression to chronic kidney disease (CKD). Serum IL-24 was detected earlier than the elevation of serum creatinine levels and urinary IL-24 was detected as early as neutrophil gelatinase associated lipocalin (NGAL) in severe AKI (60 min of IRI). In addition, serum and urine IL-24 levels tended to increase in relation to isc...
Source: Medical Molecular Morphology - December 4, 2019 Category: Molecular Biology Source Type: research

Histone H3.3 mutation in giant cell tumor of bone: an update in pathology
AbstractGiant cell tumor of bone (GCTB) is a locally aggressive bone tumor that frequently shows local recurrence and occasionally shows malignant transformation to high-grade sarcoma. Histologically, conventional GCTB is composed mainly of three types of cells: mononuclear neoplastic cells with an osteoblastic precursor phenotype, mononuclear histiocytic cells, and osteoclast-like multinucleated giant cells. These cells interact with each other via the RANKL-RANK axis and other mechanisms for tumor formation. The vast majority of GCTBs were recently revealed to harborH3F3A p.G34W mutation, and a minor subset haveH3F3A p.G...
Source: Medical Molecular Morphology - November 20, 2019 Category: Molecular Biology Source Type: research

Membranous overexpression of S100A10 is associated with a high-grade cellular status of breast carcinoma
AbstractS100A10 promotes tumor invasion in various cancers. Although genetic studies on S100A10 in breast carcinoma (BC) have been used for molecular biological classification, immunohistochemical studies are lacking. We aimed to identify the correlation between S100A10 expression in BC and various pathological parameters, including morphological features to determine histological grade (HG). Immunostained serial paraffin-embedded tissue sections from 176 cases of resected BC or normal mammary ducts (controls) were assessed for the membrane expression of S100A10. Of the 176 cases, 125 conventional infiltrating ductal carci...
Source: Medical Molecular Morphology - November 15, 2019 Category: Molecular Biology Source Type: research

Primary mediastinal large B cell lymphoma with coexisting aberrations of C-MYC and BCL-2: a case report and literature review
AbstractPrimary mediastinal large B-cell lymphoma (PMLBCL) is an aggressive lymphoma characteristic with distinct clinical, morphologic, and immunophenotypic features. The rearrangements of C-MYC, BCL2 and/or BCL6 which are common in diffuse large B-cell lymphoma (DLBCL) are typically absent in PLMBCL. Here we proved a novel case of PMLBCL with concurrent rearrangements of C-MYC and BCL2. Histology showed typical pathomorphological features of PLMBCL. Fluorescent in situ hybridization (FISH) studies showed rearrangements of C-MYC and copy number variation (CNV) of BCL2 gene. It is not well known on the clinical significanc...
Source: Medical Molecular Morphology - November 14, 2019 Category: Molecular Biology Source Type: research

Mechanism of atopic cataract caused by eosinophil granule major basic protein
AbstractAtopic cataracts develop under the ages of 40  years, after which visual acuity rapidly declines. However, the mechanism underlying the development of atopic cataracts is not yet clear. We focused on the eosinophil granule major basic protein (MBP), which was detected in the aqueous humor of atopic cataracts previously, and which was cytotoxic . Specifically, we investigated its origin in this fluid and its effects on lens epithelial cells (LECs). MBP immunostaining was positive in atopic cataract-derived LECs, but negative in age-related cataract-derived LECs.MBP mRNA was not detected in either type of catara...
Source: Medical Molecular Morphology - October 8, 2019 Category: Molecular Biology Source Type: research

An analysis of monocytes and dendritic cells differentiated from human peripheral blood monocyte-derived induced pluripotent stem cells
AbstractDendritic cell-based immunotherapy, which uses a patient's own immune cells, can be used for cancer treatment and allergy control, such as autoimmune disease and rejection associated with transplantation. However, these treatments create a burden on patients due to repeated blood collection. We used cell biological analysis of monocytes with few mutations obtained from minimal blood collection for genome recombination. Next, we established human peripheral blood monocyte-derived induced pluripotent stem cells (iPSCs) using a commercial vector and standard culture method. We found that when established iPSCs were in...
Source: Medical Molecular Morphology - October 4, 2019 Category: Molecular Biology Source Type: research

Ultrastructural characterization of cells in the tibial stump of ruptured human anterior cruciate ligament, their changes and significance with duration of injury
AbstractFibroblasts and myofibroblasts have been known to be present in both ruptured and intact human anterior cruciate ligament (ACL), and although their relevant histology and immunochemistry have been studied in the past, ultrastructural features of these cells are largely lacking. Therefore, we aim to characterise the ultrastructural details of these cells with the help of transmission electron microscopy (TEM) and to study the changes and their significance with duration of injury. Samples from 60 ruptured human ACL undergoing surgery were obtained and categorised according to duration of injury and observed under TE...
Source: Medical Molecular Morphology - September 27, 2019 Category: Molecular Biology Source Type: research

Glucocorticoid-induced leucine zipper protects noise-induced apoptosis in cochlear cells by inhibiting endoplasmic reticulum stress in rats
AbstractEndoplasmic reticulum (ER) stress-mediated apoptosis has been reported to be involved in the noise-induced hearing loss (NIHL). Glucocorticoid-induced leucine zipper (GILZ) protein has been reported to have different regulatory effects on apoptosis according to cell types. However, whether GILZ regulates apoptosis in cochlear cells is unclear. Our study aimed to investigate the mechanism by which GILZ protected ER stress-mediated cochlear apoptosis induced by noise exposure. In our trials, forty-eight male Spraque –Dawley rats were randomized into the noise, OE-GILZ-rLV + noise (ON), shRNA-GILZ-...
Source: Medical Molecular Morphology - September 4, 2019 Category: Molecular Biology Source Type: research

Expression of fatty-acid-binding protein 5 in intrahepatic and extrahepatic cholangiocarcinoma: the possibility of different energy metabolisms in anatomical location
AbstractThe biliary tract cancer (BTC) covers a range of carcinomas, including intrahepatic cholangiocarcinoma (ICC), cholangiolocellular carcinoma (CoCC), perihilar cholangiocarcinoma (perihilar CC), extrahepatic cholangiocarcinoma (ECC), and gallbladder cancer (GBC), defined according to the anatomical location. These adenocarcinomas mostly comprise biliary epithelial cell-derived malignant cells. In addition to anatomical differences, there are morphological and biological differences in BTC starting from embryonic development of the tissues extending to physiological differences. Fatty acid-binding proteins (FABPs) are...
Source: Medical Molecular Morphology - August 20, 2019 Category: Molecular Biology Source Type: research

Stage-dependent differential gene expression profiles of cranial neural crest-like cells derived from mouse-induced pluripotent stem cells
AbstractCranial neural crest cells are multipotent cells that migrate into the pharyngeal arches of the vertebrate embryo and differentiate into various craniofacial organ derivatives. Therefore, migrating cranial neural crest cells are considered one of the most attractive candidate cell sources in regenerative medicine. We generated cranial neural crest like cell (cNCCs) using mouse-induced pluripotent stem cells cultured in neural crest-inducing medium for 14  days. Subsequently, we conducted RNA sequencing experiments to analyze gene expression profiles of cNCCs at different time points after induction. cNCCs expr...
Source: Medical Molecular Morphology - July 11, 2019 Category: Molecular Biology Source Type: research

Idiopathic copper toxicosis: is abnormal copper metabolism a primary cause of this disease?
AbstractIdiopathic copper toxicosis (ICT) is characterized by marked copper deposition, Mallory-Denk body (MDB) formation and severe hepatic injury. Although the characteristics are apparently different from Wilson disease, large amounts of copper accumulate in the liver of the patients. We extensively treated a patient with ICT to reduce the body copper, however, the patient needed liver transplantation. Previous liver biopsy revealed high copper content. But extirpated liver contained an extremely small amount of copper, although MDBs and severe inflammation remained. These phenomena suggest abnormal copper metabolism is...
Source: Medical Molecular Morphology - June 3, 2019 Category: Molecular Biology Source Type: research

Three-dimensional architecture of the acetabular labrum in the human hip joint
AbstractThe acetabular labrum is frequently damaged with advancing age. As collagen fibers are the main sources of strength, knowledge of their ultrastructure is important to determine the cause of age-induced changes. We aimed to investigate the ultrastructure of collagen fibers constituting the acetabular labrum using scanning electron microscopy (SEM). Acetabular labrum samples obtained during total hip arthroplasty were studied. The samples were specially prepared to observe the steric construction of collagen fibrils constituting the acetabular labrum under light microscopy followed by SEM. The acetabular labrum was m...
Source: Medical Molecular Morphology - May 29, 2019 Category: Molecular Biology Source Type: research

Multiplex PCR analysis of apocrine lesions shows frequent PI3K –AKT pathway mutations in both benign and malignant apocrine breast tumors
AbstractPathological diagnosis of intraductal apocrine lesions can be challenging, because even benign apocrine lesions often show atypical cytology, and immunohistochemistry is of little assistance. A new diagnostic method for apocrine lesions is desirable. The mutations present in apocrine lesions have not been well studied. We performed a MassARRAY multiplex polymerase chain reaction (PCR) study of benign and malignant apocrine lesions, which included 152 mutations of 18 genes. We found that four of 11 benign lesions showedAKT1 orPIK3CA mutations, one of four noninvasive apocrine carcinomas showed aFBX4 mutation, two of...
Source: Medical Molecular Morphology - May 28, 2019 Category: Molecular Biology Source Type: research

Molecular association of FtsZ with the intrabacterial nanotransportation system for urease in Helicobacter pylori
In this study, we demonstrated by immunoelectron microscopy that urease along the route ofibNoTS localizes closely with the FtsZ filament in the bacterium. Supporting this, we found by enzyme immunoassay and co-immunoprecipitation analysis that urease interacted with FtsZ. These findings indicate that urease along the route ofibNoTS is closely associated with the FtsZ filament. Since these phenomena were not observed inibNoTS for CagA, the route ofibNoTS for CagA is different from that ofibNoTS for urease. We propose that the route ofibNoTS for urease is associated with the FtsZ filament inH. pylori. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - May 27, 2019 Category: Molecular Biology Source Type: research

Ultrastructure and three-dimensional architecture of the anterior cruciate ligament in the knee joints of young and old monkeys
We examined the ultrastructure of the anterior cruciate ligament and assessed age-related changes by comparing the ligaments of young and old monkeys. Ultrathin sections of the anterior cruciate ligament were observed by transmission electron microscopy. The three-dimensional architecture of collagen fibers in the ligament was examined by scanning electron microscopy after tissue specimens were treated with 2  N NaOH to digest the extracellular matrix. At the surface layer of the cruciate ligament in young monkeys, fusiform-shaped fibroblasts actively produced collagen fibrils. The ligament consisted of parallel bundl...
Source: Medical Molecular Morphology - May 18, 2019 Category: Molecular Biology Source Type: research

Assessment of ApoC1, LuzP6, C12orf75 and OCC-1 in cystic glioblastoma using MALDI –TOF mass spectrometry, immunohistochemistry and qRT-PCR
AbstractMass spectrometric analysis of glioblastoma cyst fluids has disclosed a protein peak withm/z 6424 –6433. Among the proteins, potentially generating this peak are ApoC1 and LuzP6. To further elucidate protein expression of glioblastoma cells, we analyzed MALDI–TOF results of cyst fluid, performed immunohistochemistry and mRNA analysis. MALDI–TOF protein extraction from 24 glioblastoma cyst fluids was performed with a weak cation exchange. 50 glioblastoma samples were stained with two custom-made antibodies against LuzP6 and commercial antibodies against ApoC1, C12orf75 and OCC-1 and analyzed. For m...
Source: Medical Molecular Morphology - April 20, 2019 Category: Molecular Biology Source Type: research

A novel splicing mutation of PTCH1 in a Chinese family with nevoid basal cell carcinoma syndrome
AbstractNevoid basal cell carcinoma syndrome (NBCCS) is a rare autosomal dominant disease characterized by the development of multiple jaw keratocysts and basal cell carcinomas (BCC) and accompanied by diverse phenotypes. The establishment of diagnosis lies on the identification of a heterozygous germline pathogenic variant in the patched homolog 1 gene (PTCH1).PTCH1 has alternative splicing and selective initial coding exon, leading to three types of encoding proteins (PTCHL, PTCHM and PTCHS). The expression of each protein in NBCCS remains ambiguous, especially the importance of the first two exons in translation. Here, ...
Source: Medical Molecular Morphology - April 17, 2019 Category: Molecular Biology Source Type: research

Assessment of CD133-positive extracellular membrane vesicles in pancreatic cancer ascites and beyond
(Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - April 5, 2019 Category: Molecular Biology Source Type: research

Heparin prevents oxidative stress-induced apoptosis in human decidualized endometrial stromal cells
AbstractClinical trials have shown that administering heparin during the luteal phase has beneficial effects on implantation and live birth rates. Heparin exerts direct effects on decidual human endometrial stromal cells (HESCs), which are independent of its anticoagulant effect. However, the accurate effects of heparin on the decidualization process remain unidentified. Here, we demonstrate that HESCs become dramatically resistant to oxidative stress upon decidualization, and we hypothesize a possible direct action of heparin on the decidualization of HESCs, which would lead to improved implantation. To test this hypothes...
Source: Medical Molecular Morphology - March 16, 2019 Category: Molecular Biology Source Type: research

Cavin-1 is linked to lipid droplet formation in human hepatic stellate cells
(Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - March 15, 2019 Category: Molecular Biology Source Type: research

Glycosylation of ascites-derived exosomal CD133: a potential prognostic biomarker in patients with advanced pancreatic cancer
This study analyzed the expression of the CSC marker CD133 in the ascites-derived exosomes obtained from patients with unresectable pancreatic cancer. In addition, inverse correlation of CD133 expression with prognosis was examined. Of the 133 consecutive patients, 19 patients were enrolled in the study. Exosomes derived from the malignant ascites demonstrated higher density and wider variation in size than those from non-malignant ascites. Western blot revealed enhanced expression of CD133 in exosomes obtained from patients with pancreatic cancer compared to those obtained from patients with gastric cancer or liver cirrho...
Source: Medical Molecular Morphology - February 25, 2019 Category: Molecular Biology Source Type: research

Relationship between genetic alterations and clinicopathological characteristics of papillary thyroid carcinoma
AbstractPapillary thyroid carcinoma (PTC) is characterized by proliferation of follicular cells with distinctive nuclear features such as ground glass appearance, nuclear groove and pseudoinclusion. From the proliferation pattern, PTC is divided into several histological subtypes; conventional histology is classified as papillary type, and there are also follicular and solid variants. PTC is heterogeneous in genetic alterations. PTC with BRAF mutation presents a histology of conventional PTC, and follows an aggressive clinical course. Most cases of PTC with RAS mutation show a follicular variant, and prognosis is favorable...
Source: Medical Molecular Morphology - February 20, 2019 Category: Molecular Biology Source Type: research

Heme oxygenase-1 prevents glucocorticoid and hypoxia-induced apoptosis and necrosis of osteocyte-like cells
The objective of this study was to evaluate the effect of HO-1 on osteocyte death caused by glucocorticoids and hypoxia. We confirmed that hemin induced HO-1 expression in MLO-Y4 mouse osteocytes. MLO-Y4 was cultured with dexamethasone (Dex) under hypoxia (DH group). Furthermore, these cells were cultured with hemin (DH-h group) or hemin and zinc protoporphyrin IX (an HO-1 inhibitor) (DH-h-PP group). The rates of apoptosis and necrosis of these groups were analyzed by flow cytometry and compared with cells cultured under normal condition. Both apoptosis and necrosis increased in the DH group. Hemin administration significa...
Source: Medical Molecular Morphology - January 31, 2019 Category: Molecular Biology Source Type: research

The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression
In this study, the therapeutic effects of RSV on hepatic oxidative stress (OS), inflammation, and lipid metabolism-related gene expression of obese mice induced by a high-fat diet (HFD) were investigated. Male C57BL/6 mice were fed a HFD for 8 weeks to induce obesity-related NAFLD model. And then, NAFLD mice were treated with daily RSV oral gavage at the dose of 100  mg/kg body weight for an additional 4 weeks. HFD-induced the elevation of serum total cholesterol, high-density lipoprotein cholesterol, glucose, insulin, aspartate aminotransferase and alanine aminotransferase levels, and homeostasis model assessment of ...
Source: Medical Molecular Morphology - January 23, 2019 Category: Molecular Biology Source Type: research

High sulfite oxidase expression could predict postoperative biochemical recurrence in patients with prostate cancer
This study was conducted using Tissue Micro Array specimens obtained from 97 patients who underwent radical prostatectomy at our hospital between 2007 and 2011. SUOX staining was used to evaluate cytoplasmic SUOX expression. In the high-expression group, the early biochemical recurrence was significantly more frequent than in the low-expression group (p = 0.0008). In multivariate analysis, high SUOX expression was found to serve as an independent prognostic factor of biochemical recurrence (hazard ratio = 2.33, 95% confidence interval = 1.32–4.15,p = 0.0037). In...
Source: Medical Molecular Morphology - January 10, 2019 Category: Molecular Biology Source Type: research

Knockdown of long non-coding RNA PCAT1 in glioma stem cells promotes radiation sensitivity
This study aimed to investigate the function of glioma stem cells (GSCs) and the role of PCAT1. This study dissociated the differences between GSCs and glioma cells in terms of apoptosis rate and γH2AX positive cells levels after radiation. Microarray was carried out to detect that expressed PCAT1, and it was testified by RT-qPCR. After transfection, GSCs were used to investigate the influence of PCAT1 on radiation sensitivity. Sphere-formation capability was first examined. Cell apoptosis rate after radiation of 0 Gy or 6 Gy was analyzed by flow cytometry, and the level of γH2AX positive cells after ...
Source: Medical Molecular Morphology - December 18, 2018 Category: Molecular Biology Source Type: research

Fgfr1 conditional-knockout in neural crest cells induces heterotopic chondrogenesis and osteogenesis in mouse frontal bones
AbstractMost facial bones, including frontal bones, are derived from neural crest cells through intramembranous ossification. Fibroblast growth factor receptor 1 (Fgfr1) plays a pivotal role in craniofacial bone development, and loss of Fgfr1 leads to cleft palate and facial cleft defects in newborn mice. However, the potential role of theFgfr1 gene in neural crest cell-mediated craniofacial development remains unclear. To investigate the role ofFgfr1 in neural crest cells, we analyzedWnt1-Cre;Fgfr1flox/flox mice. Our results show that specific knockout ofFgfr1 in neural crest cells induced heterotopic chondrogenesis and o...
Source: Medical Molecular Morphology - November 29, 2018 Category: Molecular Biology Source Type: research

Interactions between mitochondria and endoplasmic reticulum in demyelinated axons
In this study, we investigated the three-dimensional ultrastructural changes in membrane juxtapositions between mitochondria and endoplasmic reticulum in axons of a chronic progressive demyelination mouse model caused by extra copies of proteolipid protein (PLP4e). In the optic nerve of PLP4e mice, most axons were ensheathed by myelin by age 1 month, but were demyelinated by age 5 months. At age 1 month, mitochondria in PLP4e mice were slightly larger than those in wild-type mice, while the size and frequency of juxtaposition were similar. At age 5 months, the sizes of mitochondria and size of juxtaposition in PLP4e mice w...
Source: Medical Molecular Morphology - November 17, 2018 Category: Molecular Biology Source Type: research

Comparison of the characteristics of mesenchymal stem-like cells derived by integration-free induced pluripotent stem cells in different single-cell culture media under feeder-free conditions
AbstractGenerating mesenchymal stem-like cells (MSLCs) from induced pluripotent stem cells (iPSCs) can be a practical method for obtaining the sufficient cells for autologous tissue engineering. Single-cell culturing in specific medium and non-feeder cells is an alternative and promising strategy to overcome problems of embryo culture; however, little is known about how different culture media affect the proliferation and differentiation of MSLCs. We first derived MSLCs from iPSCs with non-integrating episomal plasmid vectors (hereafter 409B2 cells) using three different cell culture media, including single-cell culture me...
Source: Medical Molecular Morphology - November 16, 2018 Category: Molecular Biology Source Type: research

Oral lymphoid lesions: a 47-year clinicopathological study in a Brazilian population
AbstractWe performed an epidemiological, clinical and histopathological analysis of oral lymphoid lesions (OLLs) during a 47-year period. Data regarding patient age, sex, duration, location, symptomatology, type of growth, implantation, staining, presence of ulceration and bleeding of all cases were compiled from the clinical data. For the histopathological analyses, all slides stained by H/E were reassessed. During the analyzed period, 14,565 patients with oral and maxillofacial lesions were diagnosed, with 45 cases diagnosed as OLLs. The most prevalent location was the tongue. Females were more affected, and the mean age...
Source: Medical Molecular Morphology - October 31, 2018 Category: Molecular Biology Source Type: research

Phosphorylated HER3 and FITC-labeled trastuzumab immunohistochemistry in patients with HER2-positive breast cancer treated with adjuvant trastuzumab
AbstractThe development of trastuzumab has significantly improved the prognosis of HER2-positive breast cancer. However, disease recurs in some patients with HER2-positive breast cancer. A new strategy for treating HER2-positive breast cancer is necessary. Although several studies have reported that HER3 is a prognostic factor for HER2-positive breast cancers, phosphorylated HER3 (pHER3) has not been well studied. There has been no survival analysis including immunohistochemistry with trastuzumab as the primary antibody. We analyzed immunohistochemistry using anti-pHER3 antibody and FITC-labeled trastuzumab (FITC-tra). Of ...
Source: Medical Molecular Morphology - October 13, 2018 Category: Molecular Biology Source Type: research

Enhanced expression of nidogen 1 around the nest of basal cell carcinoma compared with that around squamous cell carcinoma
AbstractBasal cell carcinoma (BCC) is a malignant skin tumor originating from cells of the epidermal basal layer and adnexal epithelium, especially in sun-exposed areas. Unlike squamous cell carcinoma (SCC), BCC has a propensity to grow only locally possibly due to differences in the surrounding microenvironment including the basement membrane (BM) and stroma. To investigate the components constituting the BM and surrounding connective tissue in BCC and SCC, we analyzed the expression of BM proteins, nidogen 1 (NID1) and type IV collagen (COL4). We compared the immunohistochemical expressions of NID1 and COL4 among tumor s...
Source: Medical Molecular Morphology - October 1, 2018 Category: Molecular Biology Source Type: research

Comparing protein and mRNA expressions of the human epidermal growth factor receptor family in estrogen receptor-positive breast cancer
In conclusion, IHC evaluation may be reliable and representable for mRNA. Hence, this study established a marked correlation between the mRNA expressions of HER family members in patients with ER-positive breast cancer. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - September 27, 2018 Category: Molecular Biology Source Type: research

Morphological characterization of pulmonary microvascular disease in bronchopulmonary dysplasia caused by hyperoxia in newborn mice
ConclusionOur results suggest that the abnormal morphology of capillary ECs and thick BABs correlates with pulmonary artery remodeling and RVH. These ultrastructural changes might represent possible mechanisms of secondary pulmonary hypertension in BPD. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research

Serum levels of a cell death biomarker predict the development of cirrhosis-related conditions in primary biliary cholangitis
AbstractNon-invasive predictors for the development of cirrhosis-related conditions are needed for patients with primary biliary cholangitis (PBC). We investigated the association between cytokeratin-18 fragments (M30 and M65) and liver histology, treatment response and the development of cirrhosis-related conditions in patients with PBC. We retrospectively reviewed the clinical data of 111 individuals with biopsy-proven PBC. Serum M30 and M65 levels were measured using stored sera. M30 were significantly decreased after treatment, but there was no significant change in the M65 levels. M65 was significantly higher in non-r...
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research

Novel Rest functions revealed by conditional gene ablation
AbstractRest is a regulator of neuronal development and has been suggested to function in maintaining the pluripotent state of embryonic stem cells (ESCs); however, this remains controversial. SinceRest null mice show embryonic lethality, we herein generated conditionalRest knockout (CKO) models to investigate Rest functions in more detail. Our results revealed that Rest was not necessary for maintaining the pluripotency of ESCs and instead promoted primitive endoderm differentiation. In contrast to the repressive role of Rest in vitro, including ESCs, neural stem cells, and fibroblasts, on the expression of target neural ...
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research

Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney
AbstractAlthough an association between periodontitis and chronic kidney disease (CKD) has been suggested, the mechanism involved remains unclear. Herein, we examined the global gene expression profile in a mouse model that showed no acute inflammation in the kidney following stimulation with lipopolysaccharides (LPS) derived fromPorphyromonas gingivalis (PG-LPS). The mice were injected with PG-LPS at a concentration of 5  mg/kg intraperitoneally, every 3 days, for 1 month. Microarray analysis was used to identify 10 genes with the highest expression levels in the kidney stimulated with PG-LPS. Among them, t...
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research

Characteristics of pericytes in diethylstilbestrol (DES)-induced pituitary prolactinoma in rats
This study is the first to clarify fine structural changes of pericytes in rat prolactinomas and improves our understanding of the function of pericytes under pathological conditions. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research

Morphological and molecular characterization of the senile osteoporosis in senescence-accelerated mouse prone 6 (SAMP6)
AbstractAlthough the understanding of the complex pathogenesis for osteoporosis is appreciable, the underlying mechanism is not yet fully elucidated. There is a great need to further characterize the available animal models in osteoporosis research. The senescence-accelerated mouse prone 6 (SAMP6) mice have been developed as the spontaneous experimental model for senile osteoporosis. Here, we provide a comprehensive overview of current research regarding the bone morphological and molecular alterations and the possible mechanisms involved in these changes. There were significant decrease in trabecular bone mass at the axia...
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research

Protective role of heme oxygenase-1 in fatty liver ischemia –reperfusion injury
AbstractIschemia –reperfusion (IR) injury is a kind of injury resulting from the restoration of the blood supply after blood vessel closure during liver transplantation and is the main cause of graft failure. The pathophysiological mechanisms of hepatic IR include a variety of oxidative stress responses. Hepatic I R is characterized by ischemia and hypoxia inducing oxidative stress, immune response and apoptosis. Fat-denatured livers are also used as donors due to the lack of liver donors. Fatty liver is less tolerant to IR than normal liver. Heme oxygenase (HO) is an enzyme that breaks down hemoglobin to bil irubin,...
Source: Medical Molecular Morphology - August 31, 2018 Category: Molecular Biology Source Type: research

Evaluation of immunohistochemical expression of survivin and its correlation with −31G/C gene polymorphism in colorectal cancer
AbstractColorectal cancer (CRC) placed among the most common neoplasm. Survivin is a member of the inhibitor apoptosis gene family. This gene could be associated with aggressive behavior in numerous types of cancers. The aim of the present study was to evaluate the immunohistochemical expression of survivin gene and its correlation with −31G/C polymorphism in CRC patients. This case–control study was performed on 90 cases: 30 adenocarcinoma, 30 adenomatous polyp, and 30 normal colon. Immunohistochemical expression of survivin evaluated on formalin-fixed paraffin-embedded tissue and −31G/C polymorphism was...
Source: Medical Molecular Morphology - August 20, 2018 Category: Molecular Biology Source Type: research