Enhanced expression of nidogen 1 around the nest of basal cell carcinoma compared with that around squamous cell carcinoma
AbstractBasal cell carcinoma (BCC) is a malignant skin tumor originating from cells of the epidermal basal layer and adnexal epithelium, especially in sun-exposed areas. Unlike squamous cell carcinoma (SCC), BCC has a propensity to grow only locally possibly due to differences in the surrounding microenvironment including the basement membrane (BM) and stroma. To investigate the components constituting the BM and surrounding connective tissue in BCC and SCC, we analyzed the expression of BM proteins, nidogen 1 (NID1) and type IV collagen (COL4). We compared the immunohistochemical expressions of NID1 and COL4 among tumor s...
Source: Medical Molecular Morphology - October 1, 2018 Category: Molecular Biology Source Type: research
Comparing protein and mRNA expressions of the human epidermal growth factor receptor family in estrogen receptor-positive breast cancer
In conclusion, IHC evaluation may be reliable and representable for mRNA. Hence, this study established a marked correlation between the mRNA expressions of HER family members in patients with ER-positive breast cancer. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - September 27, 2018 Category: Molecular Biology Source Type: research
Morphological characterization of pulmonary microvascular disease in bronchopulmonary dysplasia caused by hyperoxia in newborn mice
ConclusionOur results suggest that the abnormal morphology of capillary ECs and thick BABs correlates with pulmonary artery remodeling and RVH. These ultrastructural changes might represent possible mechanisms of secondary pulmonary hypertension in BPD. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research
Serum levels of a cell death biomarker predict the development of cirrhosis-related conditions in primary biliary cholangitis
AbstractNon-invasive predictors for the development of cirrhosis-related conditions are needed for patients with primary biliary cholangitis (PBC). We investigated the association between cytokeratin-18 fragments (M30 and M65) and liver histology, treatment response and the development of cirrhosis-related conditions in patients with PBC. We retrospectively reviewed the clinical data of 111 individuals with biopsy-proven PBC. Serum M30 and M65 levels were measured using stored sera. M30 were significantly decreased after treatment, but there was no significant change in the M65 levels. M65 was significantly higher in non-r...
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research
Novel Rest functions revealed by conditional gene ablation
AbstractRest is a regulator of neuronal development and has been suggested to function in maintaining the pluripotent state of embryonic stem cells (ESCs); however, this remains controversial. SinceRest null mice show embryonic lethality, we herein generated conditionalRest knockout (CKO) models to investigate Rest functions in more detail. Our results revealed that Rest was not necessary for maintaining the pluripotency of ESCs and instead promoted primitive endoderm differentiation. In contrast to the repressive role of Rest in vitro, including ESCs, neural stem cells, and fibroblasts, on the expression of target neural ...
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research
Effect of systemic administration of lipopolysaccharides derived from Porphyromonas gingivalis on gene expression in mice kidney
In conclusion, the responses noted in the kidney may have arisen mainly from the endothelial cells. Moreover, upregulation of the expression levels ofSaa3, Ticam2, Reg3b, Ocxt2a, andXcr1 may be associated with the pathogenesis of CKD. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research
Characteristics of pericytes in diethylstilbestrol (DES)-induced pituitary prolactinoma in rats
This study is the first to clarify fine structural changes of pericytes in rat prolactinomas and improves our understanding of the function of pericytes under pathological conditions. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research
Morphological and molecular characterization of the senile osteoporosis in senescence-accelerated mouse prone 6 (SAMP6)
AbstractAlthough the understanding of the complex pathogenesis for osteoporosis is appreciable, the underlying mechanism is not yet fully elucidated. There is a great need to further characterize the available animal models in osteoporosis research. The senescence-accelerated mouse prone 6 (SAMP6) mice have been developed as the spontaneous experimental model for senile osteoporosis. Here, we provide a comprehensive overview of current research regarding the bone morphological and molecular alterations and the possible mechanisms involved in these changes. There were significant decrease in trabecular bone mass at the axia...
Source: Medical Molecular Morphology - September 1, 2018 Category: Molecular Biology Source Type: research
Protective role of heme oxygenase-1 in fatty liver ischemia –reperfusion injury
AbstractIschemia –reperfusion (IR) injury is a kind of injury resulting from the restoration of the blood supply after blood vessel closure during liver transplantation and is the main cause of graft failure. The pathophysiological mechanisms of hepatic IR include a variety of oxidative stress responses. Hepatic I R is characterized by ischemia and hypoxia inducing oxidative stress, immune response and apoptosis. Fat-denatured livers are also used as donors due to the lack of liver donors. Fatty liver is less tolerant to IR than normal liver. Heme oxygenase (HO) is an enzyme that breaks down hemoglobin to bil irubin, fer...
Source: Medical Molecular Morphology - August 31, 2018 Category: Molecular Biology Source Type: research
Evaluation of immunohistochemical expression of survivin and its correlation with −31G/C gene polymorphism in colorectal cancer
AbstractColorectal cancer (CRC) placed among the most common neoplasm. Survivin is a member of the inhibitor apoptosis gene family. This gene could be associated with aggressive behavior in numerous types of cancers. The aim of the present study was to evaluate the immunohistochemical expression of survivin gene and its correlation with −31G/C polymorphism in CRC patients. This case–control study was performed on 90 cases: 30 adenocarcinoma, 30 adenomatous polyp, and 30 normal colon. Immunohistochemical expression of survivin evaluated on formalin-fixed paraffin-embedded tissue and −31G/C polymorphism was analyzed by...
Source: Medical Molecular Morphology - August 20, 2018 Category: Molecular Biology Source Type: research
FGF-2 combined with bilayer artificial dermis composed of collagen matrix prompts generation of fat pad in subcutis of mice
This study has identified a promising method to enhance adipogenic effects in the murine subcutis, representing a potential technique for soft tissue reconstruction. (Source: Medical Molecular Morphology)
Source: Medical Molecular Morphology - July 18, 2018 Category: Molecular Biology Source Type: research
