The expression of MYC is strongly dependent on the circular PVT1 expression in pure Gleason pattern 4 of prostatic cancer

AbstractPVT1 is a long-noncoding RNA and is highly expressed in various cancers including prostate cancers with stabilizing MYC protein. To characterize the objective biological features of the different morphological components such as Gleason patterns (GP) in prostate cancer, biopsy specimens containing only single pure GP (GP3, GP4, GP5) are used to analyze the relationship betweenPVT1 expression and MYC protein expression. The expressions ofPVT1 andMYC were analyzed by quantitative PCR and the labeling index (LI) of MYC protein by immunohistochemical staining.PVT1,MYC, and MYC protein were highly expressed in GP 4, and interestingly the expression betweenPVT1 and MYC LI significantly correlated only in GP 4. In vitro experiments, the expression of MYC protein was slightly reduced by small interfering RNA againstPVT1, while strongly reduced against specifically circularPVT1, splicing variants derived from thePVT1. Taken together, the expression characteristics ofPVT1,MYC, and MYC protein differed depending on the GP. In particular, circularPVT1 might be strongly involved in the stabilization of MYC protein in GP4 and suggest different biological features.
Source: Medical Molecular Morphology - Category: Molecular Biology Source Type: research