Chronic stress-induced elevation of IL-1 β in the saliva and submandibular glands of mice

We examined the effect of chronic stress on three major inflammatory cytokines Interleukin (IL)-1 β, IL-6, and tumor necrosis factor (TNF)-α in the saliva and salivary glands of chronically stressed mice. Six-week-old C57BL/6 J mice were randomly divided into a control and a stress group. The mice in stress group were exposed to 4 h of stress daily for 10 days and subsequently saliva, as we ll as the submandibular glands, were collected from both groups. The expression levels of cytokines in the saliva were examined by enzyme-linked immunosorbent assay. The submandibular glands were subjected to histopathological and mRNA expression analyses. IL-1β was significantly elevated in saliva of the chronic stressed mice. Furthermore, the mRNA expression levels of bothIL-1 β andIL-6 were significantly elevated in the submandibular gland of chronic stressed mice. IL-1 β may be a potential salivary biomarker in response to chronic stress in mice.
Source: Medical Molecular Morphology - Category: Molecular Biology Source Type: research