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Photoacoustic microscopy based on transparent piezoelectric ultrasound transducers
Journal of Innovative Optical Health Sciences, Ahead of Print. Photoacoustic microscopy (PAM), due to its deep penetration depth and high contrast, is playing an increasingly important role in biomedical imaging. PAM imaging systems equipped with conventional ultrasound transducers have demonstrated excellent imaging performance. However, these opaque ultrasonic transducers bring some constraints to the further development and application of PAM, such as complex optical path, bulky size, and difficult to integrate with other modalities. To overcome these problems, ultrasonic transducers with high optical transparency have ...
Source: Journal of Innovative Optical Health Sciences - February 11, 2023 Category: Biomedical Science Authors: Hangbing Peng Zhongwen Cheng Lvming Zeng Xuanrong Ji Source Type: research

Fluorescence imaging analysis of the glioma microenvironment
Journal of Innovative Optical Health Sciences, Ahead of Print. Glioma is the most malignant brain cancer. The neurons, macrophages, T cells and other immune cells constitute the glioma immunosuppressive microenvironment. The accurate spatial distribution of these cells in the glioma microenvironment and its relationship with glioma metastasis is unknown. We constructed a mouse glioma cell line stably expressing the large Stokes-shifted yellow fluorescent protein and applied it to the multicolor immunofluorescence imaging. The imaging data revealed that the neurons were sparsely distributed in the glioma core and the number...
Source: Journal of Innovative Optical Health Sciences - February 6, 2023 Category: Biomedical Science Authors: Xuwen Peng Yuzhou Chen Yuke Wang Shuhong Qi Source Type: research

A graphical user interface (GUI) for model-based radiation-induced acoustic computed tomography
Journal of Innovative Optical Health Sciences, Ahead of Print. Radiation-induced acoustic computed tomography (RACT) is an evolving biomedical imaging modality that aims to reconstruct the radiation energy deposition in tissues. Traditional back-projection (BP) reconstructions carry noisy and limited-view artifacts. Model-based algorithms have been demonstrated to overcome the drawbacks of BPs. However, model-based algorithms are relatively more complex to develop and computationally demanding. Furthermore, while a plethora of novel algorithms has been developed over the past decade, most of these algorithms are either not...
Source: Journal of Innovative Optical Health Sciences - February 6, 2023 Category: Biomedical Science Authors: Michelle Simon Prabodh Kumar Pandey Leshan Sun Liangzhong Xiang Source Type: research

Observing single cells in whole organs with optical imaging
Journal of Innovative Optical Health Sciences, Ahead of Print. Cells are the basic unit of human organs that are not fully understood. The revolutionary advancements of optical imaging allowed us to observe single cells in whole organs, revealing the complicated composition of cells with spatial information. Therefore, in this review, we revisit the principles of optical contrast related to those biomolecules and the optical techniques that transform optical contrast into detectable optical signals. Then, we describe optical imaging to achieve three-dimensional spatial discrimination for biological tissues. Due to the milk...
Source: Journal of Innovative Optical Health Sciences - February 4, 2023 Category: Biomedical Science Authors: Xiaoquan Yang Tao Jiang Lirui Liu Xiaojun Zhao Ximiao Yu Minjun Yang Guangcai Liu Qingming Luo Source Type: research

Introduction to the special issue on celebrating the 15th anniversary of JIOHS and the 70th anniversary of HUST
Journal of Innovative Optical Health Sciences, Ahead of Print. (Source: Journal of Innovative Optical Health Sciences)
Source: Journal of Innovative Optical Health Sciences - February 3, 2023 Category: Biomedical Science Authors: Qingming Luo Valery V. Tuchin Lihong Wang Source Type: research

Effect of laser acupuncture combined with electrical stimulation on recovery from exercise fatigue in mice
Journal of Innovative Optical Health Sciences, Ahead of Print. In sports events, the rapid recovery after high-intensity training or sport competition performance is very important for athletes ’ performance and health. The aim of this study is to evaluate the effect of laser acupuncture and electrical stimulation on the recovery from exercise fatigue, using mice with swimming fatigue as experimental model and the electromyography (EMG) and the Raman spectroscopy of blood as evaluation i ndicators. Root mean square (RMS) and mean power frequency (MPF) of EMG were analyzed after laser acupuncture and electrical stimulatio...
Source: Journal of Innovative Optical Health Sciences - January 25, 2023 Category: Biomedical Science Authors: Shupeng Liu Yudan Xia Zehai Hou Haotian Hong Na Chen Taihao Li Chunlei Shan Heng Zhang Source Type: research

A digital microfluidic single-cell manipulation system optimized by extending-depth-of-field device
Journal of Innovative Optical Health Sciences, Ahead of Print. Microfluidic systems have been widely utilized in high-throughput biology analysis, but the difficulties in liquid manipulation and cell cultivation limit its application. This work has developed a new digital microfluidic (DMF) system for on-demand droplet control. By adopting an extending-depth-of-field (EDoF) phase modulator to the optical system, the entire depth of the microfluidic channel can be covered in one image without any refocusing process, ensuring that 95% of the particles in the droplet are captured within three shots together with shaking proce...
Source: Journal of Innovative Optical Health Sciences - January 25, 2023 Category: Biomedical Science Authors: Qiushu Chen Qi Meng Yuzhe Liu Xiangan Long Yawei Kong Longfang Yao Liwen Chen Chuanyong Wu Kaiqin Chu Lan Mi Jiong Ma Source Type: research

Light field microscopy in biological imaging
Journal of Innovative Optical Health Sciences, Ahead of Print. Light field microscopy (LFM), featured for high three-dimensional imaging speed and low phototoxicity, has emerged as a technique of choice for instantaneous volumetric imaging. In contrast with other scanning-based three-dimensional (3D) imaging approaches, LFM enables to encode 3D spatial information in a snapshot manner, permitting high-speed 3D imaging that is only limited by the frame rate of the camera. In this review, we first introduce the fundamental theory of LFM and current corresponding advanced approaches. Then, we summarize various applications of...
Source: Journal of Innovative Optical Health Sciences - January 20, 2023 Category: Biomedical Science Authors: Chengqiang Yi Lanxin Zhu Dongyu Li Peng Fei Source Type: research

Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy
Journal of Innovative Optical Health Sciences, Ahead of Print. The cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery, which begins before the onset of the preterm labor process. Therefore, it is important to resolve the changes of collagen fibers during cervical remodeling for the prevention of preterm labor. Herein, we assessed the spatial organization of collagen fibers in a three-dimensional (3D) context within cervical tissues of mice on day 3, 9, 12, 15 and 18 of gestation. We found that the 3D directional variance, ...
Source: Journal of Innovative Optical Health Sciences - January 18, 2023 Category: Biomedical Science Authors: Lingxi Zhou Rushan Jiang Jia Meng Shuhao Qian Shenyi Jiang Chuncheng Wang Chen Yang Zhihua Ding Zheyue Shu Zhiyi Liu Source Type: research

Using optical coherence tomography images to evaluate fungal growth in reline resins
Journal of Innovative Optical Health Sciences, Ahead of Print. As changes in hard or soft oral tissues normally have a microbiological component, it is important to develop diagnostic techniques that support clinical evaluation, without destroying microbiological formation. The optical coherence tomography (OCT) represents an alternative to analyze tissues and microorganisms without the need for processing. This imaging technique could be defined as a fast, real-time, in situ, and non-destructive method. Thus, this study proposed the use of the OCT to visualize biofilm by Candida albicans in reline resins for removable pro...
Source: Journal of Innovative Optical Health Sciences - January 14, 2023 Category: Biomedical Science Authors: Mayra M. Aquino Caio B. S. Maior Nath ália A. E. Lins Cl áudia C. B. O. Mota Patricia L. A. Nascimento Anderson S. L. Gomes Source Type: research