Investigation of optical reflectance from different animal vertebra along the fixation trajectory of pedicle screw in frequency domain
Journal of Innovative Optical Health Sciences, Ahead of Print. Accurate placement of pedicle screw (PS) is crucial in spinal surgery. Developing new real-time intra-operative monitoring and navigation methods is an important direction of clinical application research. In this paper, we studied the spectrum along the fixation trajectory of PS in frequency domain to tackle the accuracy problem. Fresh porcine vertebrae, bovine vertebrae and ovine vertebrae were measured with the near-infrared spectrum (NIR) device to obtain the reflected spectrum from the vertebrae. Along the fixation trajectory of PS, average energy from dif...
Source: Journal of Innovative Optical Health Sciences - December 12, 2019 Category: Biomedical Science Authors: Yangyang Liu Huan Zhang Ying Tong Zhiyu Qian Weitao Li Source Type: research

Review of methods to improve the performance of linear array-based photoacoustic tomography
Journal of Innovative Optical Health Sciences, Ahead of Print. As a hybrid imaging modality that combines optical excitation with acoustic detection, photoacoustic tomography (PAT) has become one of the fastest growing biomedical imaging modalities. Among various types of transducer arrays used in a PAT system configuration, the linear array is the most commonly utilized due to its convenience and low-cost. Although linear array-based PAT has been quickly developed within the recent decade, there are still two major challenges that impair the overall performance of the PAT imaging system. The first challenge is that the th...
Source: Journal of Innovative Optical Health Sciences - December 6, 2019 Category: Biomedical Science Authors: Yuehang Wang Ye Zhan Melinda Tiao Jun Xia Source Type: research

Author index Volume 12 (2019)
Journal of Innovative Optical Health Sciences,Volume 12, Issue 06, November 2019. (Source: Journal of Innovative Optical Health Sciences)
Source: Journal of Innovative Optical Health Sciences - November 21, 2019 Category: Biomedical Science Source Type: research

Three-dimensional imaging of spatio-temporal dynamics of small blood capillary network in the cortex based on optical coherence tomography: A review
Journal of Innovative Optical Health Sciences, Ahead of Print. Optical coherence tomography angiography (OCTA) has emerged as an advanced in vivo imaging modality, which is widely used for the clinic ophthalmology and neuroscience research in the rodent brain cortex among others. Based on the high numerical aperture (NA) probing lens and the motion-corrected algorithms, a high-resolution imaging technique called OCT micro-angiography is applied to resolve the small blood capillary vessels ranging from 5[math][math]m to 10[math][math]m in diameter. As OCT-based techniques are recently evolving further from the structural im...
Source: Journal of Innovative Optical Health Sciences - November 12, 2019 Category: Biomedical Science Authors: Fangjian Xing Jang-Hoon Lee Collin Polucha Jonghwan Lee Source Type: research

Simplifying the detection of optical distortions by machine learning
Journal of Innovative Optical Health Sciences, Ahead of Print. Adaptive optics has been widely used in biological science to recover high-resolution optical image deep into the tissue, where optical distortion detection with high speed and accuracy is strongly required. Here, we introduce convolutional neural networks, one of the most popular machine learning models, into Shack –Hartmann wavefront sensor (SHWS) to simplify optical distortion detection processes. Without image segmentation or centroid positioning algorithm, the trained network could estimate up to 36th Zernike mode coefficients directly from a full SH...
Source: Journal of Innovative Optical Health Sciences - October 18, 2019 Category: Biomedical Science Authors: Shuwen Hu Lejia Hu Biwei Zhang Wei Gong Ke Si Source Type: research

Discrimination of cervical cancer cells via cognition-based features
Journal of Innovative Optical Health Sciences, Ahead of Print. Computer-assisted cervical screening is an effective method to save the doctors ’ workload and improve their work efficiency. Usually, the correct classification of cervical cells depends on the nuclear segmentation effect and the extraction of nuclear features. However, the precise nucleus segmentation remains a huge challenge, especially for densely distributed nucleus. Mor eover, previous cellular classification methods are mostly based on morphological features of nucleus size or color. Those individual features can make accurate classification for se...
Source: Journal of Innovative Optical Health Sciences - October 7, 2019 Category: Biomedical Science Authors: Yue Liu Jiabo Ma Xu Li Xiuli Liu Gong Rao Jing Tian Jingya Yu Shenghua Cheng Shaoqun Zeng Li Chen Junbo Hu Source Type: research

Multimodal intravascular imaging technology for characterization of atherosclerosis
Journal of Innovative Optical Health Sciences, Ahead of Print. Early detection of vulnerable plaques is the critical step in the prevention of acute coronary events. Morphology, composition, and mechanical property of a coronary artery have been demonstrated to be the key characteristics for the identification of vulnerable plaques. Several intravascular multimodal imaging technologies providing co-registered simultaneous images have been developed and applied in clinical studies to improve the characterization of atherosclerosis. In this paper, the authors review the present system and probe designs of representative intr...
Source: Journal of Innovative Optical Health Sciences - October 7, 2019 Category: Biomedical Science Authors: Yan Li Jason Chen Zhongping Chen Source Type: research

Super-resolution microscopy based on parallel detection
Journal of Innovative Optical Health Sciences, Ahead of Print. Image scanning microscopy based on pixel reassignment can improve the confocal resolution limit without losing the image signal-to-noise ratio (SNR) greatly [C.  J. R. Sheppard, “Super-resolution in confocal imaging,” Optik 80(2) 53–54 (1988). C. B. Müller, E. Jörg, “Image scanning microscopy, “Phys. Rev. Lett. 104(19) 198101 (2010). C. J. R. Sheppard, S. B. Mehta, R. Heintzmann, “Superresolution by image scanning microscopy using p ixel reassignment,” Opt...
Source: Journal of Innovative Optical Health Sciences - October 2, 2019 Category: Biomedical Science Authors: Zhimin Zhang Shaocong Liu Liang Xu Yubing Han Cuifang Kuang Yong Liu Xiang Hao Hongqin Yang Xu Liu Source Type: research

Recent advancement of chemical imaging in pharmaceutical quality control: From final product testing to industrial utilization
Journal of Innovative Optical Health Sciences, Ahead of Print. Chemical imaging (CI) possesses a strong ability of pharmaceutical analysis. Its great strength relies on the integration of traditional spectroscopy (one dimension) and imaging technique (two dimensions) to generate three-dimensional data hypercubes. Data pre-processing or processing methods are proposed to analyze vast data matrixes and thereby realizing different research objectives. In this review paper, various pharmaceutical applications of quality control over the past few years are summed up in two groups of final product test and industrial utilization...
Source: Journal of Innovative Optical Health Sciences - September 30, 2019 Category: Biomedical Science Authors: Luming Liu Haibin Qu Source Type: research

Transformational change in the field of diffuse optics: From going bananas to going nuts
Journal of Innovative Optical Health Sciences, Ahead of Print. The concept of region of sensitivity is central to the field of diffuse optics and is closely related to the Jacobian matrix used to solve the inverse problem in imaging. It is well known that, in diffuse reflectance, the region of sensitivity associated with a given source –detector pair is shaped as a banana, and features maximal sensitivity to the portions of the sample that are closest to the source and the detector. We have recently introduced a dual-slope (DS) method based on a special arrangement of two sources and two detectors, which results in d...
Source: Journal of Innovative Optical Health Sciences - September 30, 2019 Category: Biomedical Science Authors: Sergio Fantini Giles Blaney Angelo Sassaroli Source Type: research

Introduction to the Special Issue on Fluorescence Lifetime Imaging Microscopy (FLIM)
Journal of Innovative Optical Health Sciences,Volume 12, Issue 05, September 2019. (Source: Journal of Innovative Optical Health Sciences)
Source: Journal of Innovative Optical Health Sciences - September 17, 2019 Category: Biomedical Science Authors: Junle Qu Liwei Liu Source Type: research

Application of functional near-infrared spectroscopy in the healthcare industry: A review
This study comprehensively reviews brain-impaired patients: The studies involving patient populations and the diseases discussed in more than 10 works are included. Eleven diseases examined in this paper include autism spectrum disorder, attention-deficit hyperactivity disorder, epilepsy, depressive disorders, anxiety and panic disorder, schizophrenia, mild cognitive impairment, Alzheimer ’s disease, Parkinson’s disease, stroke, and traumatic brain injury. For each disease, the tasks used for examination, fNIRS variables, and significant findings on the impairment are discussed. The channel configurations and t...
Source: Journal of Innovative Optical Health Sciences - September 12, 2019 Category: Biomedical Science Authors: Keum-Shik Hong M. Atif Yaqub Source Type: research

Overexpression of Myocardin-Related Transcription Factor-A Attenuated Middle Cerebral Artery Occlusion/Reperfusion-Induced Apoptosis Via the Mcl-1/Cyt C/Cleaved Caspase 3 Pathway
Journal of Innovative Optical Health Sciences, Ahead of Print. To investigate the effect of Myocardin-related transcription factor A (MRTF-A) on apoptosis induced by ischemic/reperfusion (I/R), middle cerebral artery occlusion /reperfusion (MCAO/R) in rats were applied to mimic I/R. The neurological deficit score, cerebral infarct size, cortical neuron apoptosis and cleaved caspase 3 level were evaluated to determine the effect and the level of apoptosis by TTC straining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) straining, Western blot and immunofluorescence staining. The myeloid cell leukemia-1...
Source: Journal of Innovative Optical Health Sciences - August 26, 2019 Category: Biomedical Science Authors: Zhi-Jun Yu Jing Du Wei Zhang Shu-Qi Zhao Wei Dong Shi-Qiang Xu Ling Hu Zhen-Li Min Qiong Yuan Chunxiang Zhang Xia-Min Hu Source Type: research

Classifiers fusion for improved vessel recognition with application in quantification of generalized arteriolar narrowing
Journal of Innovative Optical Health Sciences, Ahead of Print. This paper attempts to estimate diagnostically relevant measure, i.e., Arteriovenous Ratio with an improved retinal vessel classification using feature ranking strategies and multiple classifiers decision-combination scheme. The features exploited for retinal vessel characterization are based on statistical measures of histogram, different filter responses of images and local gradient information. The feature selection process is based on two feature ranking approaches (Pearson Correlation Coefficient technique and Relief-F method) to rank the features followed...
Source: Journal of Innovative Optical Health Sciences - August 21, 2019 Category: Biomedical Science Authors: Xiaoxia Yin Samra Irshad Yanchun Zhang Source Type: research

Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues
Journal of Innovative Optical Health Sciences, Ahead of Print. The endogenous fluorophores such as reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) in cells and tissues can be imaged by fluorescence lifetime imaging microscopy (FLIM) to show the tissue morphology features, as well as the biomolecular changes in microenvironment. The two important coenzymes in cellular metabolism, NAD(P)H and FAD, can be used to monitor the cellular metabolic status. This work proposed a novel method to study the uterine metabolism at the adjacent site of healthy cervix. It was found that...
Source: Journal of Innovative Optical Health Sciences - August 5, 2019 Category: Biomedical Science Authors: Maojia Huang Zixiao Zhang Xinyi Wang Yonghui Xie Yiyan Fei Jiong Ma Jing Wang Li Chen Lan Mi Yulan Wang Source Type: research

Active wavefront shaping for controlling and improving multimode fiber sensor
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. Wavefront shaping (WFS) techniques have been used as a powerful tool to control light propagation in complex media, including multimode fibers. In this paper, we propose a new application of WFS for multimode fiber-based sensors. The use of a single multimode fiber alone, without any special fabrication, as a sensor based on the light intensity variations is not an easy task. The twist effect on multimode fiber is used as an example herein. Experimental results show that light intensity through the multimode fiber shows no direct relationship wit...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Tianting Zhong Zhipeng Yu Huanhao Li Zihao Li Haohong Li Puxiang Lai Source Type: research

Detection and localization of the hemoglobin and collagen distribution of the uterine cervix
In this study, we developed a colposcopy combining cross-polarized image and image processing algorithm with an efficient analytical model to map the distribution of blood and collagen in the uterine. For this system ’s proof of concept, we captured and processed the case of cervical ectopy and Nabothian cyst. The results show that the distribution of blood and collagen maps matched with anatomical and physiological when compared with Lugol’s iodine images. This technology has some advantages, such as low co st, real time, and can replace the use of acetic acid or Lugol’s iodine in the future. (Source: Jo...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Tran Van Tien Nguyen Ngoc Quynh Le Huynh Duc Phan Ngoc Khuong Cat Huynh Quang Linh Source Type: research

Observation of the early blood vessels of cutaneous malignant melanoma using Swept Source Optical Coherence Tomography Angiography (SS-OCTA)
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. Melanoma, characterized by high mortality, rapid development and accompanied with angiogenesis is the most typical malignant tumor in skin cancer. Hence, the detection of blood vessels is of much significance. The early vascular network has small scale. If we remove the tumor early and biopsy it, it will increase the spread of the cancer cells and infection and bleeding. In this case, we presented a new angiography method. A high-resolution OCT system for noninvasive angiographic imaging of early skin melanoma  — Swept Source Optical C...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Jingxuan Liu Jinyu Fan Quan Wang Wen He Caihua Dong Minxuan Sun Guohua Shi Source Type: research

A thorough study on genetic algorithms in feedback-based wavefront shaping
This study is beneficial in improving the performance of wavefront shaping systems with GAs. (Source: Journal of Innovative Optical Health Sciences)
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Daixuan Wu Jiawei Luo Zhaohui Li Yuecheng Shen Source Type: research

Multidither coherent optical adaptive technique for deep tissue two-photon microscopy
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. Two-photon microscopy normally suffers from the scattering of the tissue in biological imaging. Multidither coherent optical adaptive technique (COAT) can correct the scattered wavefront in parallel. However, the determination of the corrective phases may not be completely accurate using conventional method, which undermines the performance of this technique. In this paper, we theoretically demonstrate a method that can obtain more accurate corrective phases by determining the phase values from the square root of the fluorescence signal. A numeri...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Biwei Zhang Wei Gong Chenxue Wu Lejia Hu Xinpei Zhu Ke Si Source Type: research

A comparative study of optimization algorithms for wavefront shaping
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. By manipulating the phase map of a wavefront of light using a spatial light modulator, the scattered light can be sharply focused on a specific target. Several iterative optimization algorithms for obtaining the optimum phase map have been explored. However, there has not been a comparative study on the performance of these algorithms. In this paper, six optimization algorithms for wavefront shaping including continuous sequential, partitioning algorithm, transmission matrix estimation method, particle swarm optimization, genetic algorithm (GA), ...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Zahra Fayyaz Nafiseh Mohammadian M. Reza Rahimi Tabar Rayyan Manwar Kamran Avanaki Source Type: research

Seeing through dynamics scattering media: Suppressing diffused reflection based on decorrelation time difference
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. We observed a phenomenon that different scattering components have different decorrelation time. Based on decorrelation time difference, we proposed a method to image an object hidden behind a turbid medium in a reflection mode. In order to suppress the big disturbance caused by reflection and back scattering, two frames of speckles are recorded in sequence, and their difference is used for image reconstruction. Our method is immune to both medium motions and object movements. (Source: Journal of Innovative Optical Health Sciences)
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Honglin Liu Xin Wang Jingjing Gao Tingyi Yu Shensheng Han Source Type: research

Fast optical wavefront engineering for controlling light propagation in dynamic turbid media
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. While propagating inside the strongly scattering biological tissue, photons lose their incident directions beyond one transport mean free path (TMFP, [math]1 millimeter (mm)), which makes it challenging to achieve optical focusing or clear imaging deep inside tissue. By manipulating many degrees of the incident optical wavefront, the latest optical wavefront engineering (WFE) technology compensates the wavefront distortions caused by the scattering media and thus is toward breaking this physical limit, bringing bright perspective to many applicat...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Meiyun Xia Deyu Li Ling Wang Daifa Wang Source Type: research

Artificial intelligence-assisted light control and computational imaging through scattering media
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. Coherent optical control within or through scattering media via wavefront shaping has seen broad applications since its invention around 2007. Wavefront shaping is aimed at overcoming the strong scattering, featured by random interference, namely speckle patterns. This randomness occurs due to the refractive index inhomogeneity in complex media like biological tissue or the modal dispersion in multimode fiber, yet this randomness is actually deterministic and potentially can be time reversal or precompensated. Various wavefront shaping approaches...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Shengfu Cheng Huanhao Li Yunqi Luo Yuanjin Zheng Puxiang Lai Source Type: research

Exploiting the point spread function for optical imaging through a scattering medium based on deconvolution method
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. Visual perception of humans penetrating turbid medium is hampered by scattering. Various techniques have been prompted recently to recover optical imaging through turbid materials. Among them, speckle correlation based on deconvolution is one of the most attractive methods taking advantage of high imaging quality, robustness, ease-of-use, and ease-of-integration. By exploiting the point spread function (PSF) of the scattering system, large Field-of-View, extended Depth-of-Field, noninvasiveness and spectral resoluation are now available as succes...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Hexiang He Xiangsheng Xie Yikun Liu Haowen Liang Jianying Zhou Source Type: research

Overcoming the penetration depth limit in optical microscopy: Adaptive optics and wavefront shaping
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. Despite the unique advantages of optical microscopy for molecular specific high resolution imaging of living structure in both space and time, current applications are mostly limited to research settings. This is due to the aberrations and multiple scattering that is induced by the inhomogeneous refractive boundaries that are inherent to biological systems. However, recent developments in adaptive optics and wavefront shaping have shown that high resolution optical imaging is not fundamentally limited only to the observation of single cells, but ...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Cheolwoo Ahn Byungjae Hwang Kibum Nam Hyungwon Jin Taeseong Woo Jung-Hoon Park Source Type: research

Editorial: Introduction to the special issue on high-resolution optical focusing and imaging within or through thick scattering media
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. (Source: Journal of Innovative Optical Health Sciences)
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Puxiang Lai YongKeun Park Source Type: research

Active wavefront shaping for controlling and improving multimode fiber sensor
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. Wavefront shaping (WFS) techniques have been used as a powerful tool to control light propagation in complex media, including multimode fibers. In this paper, we propose a new application of WFS for multimode fiber-based sensors. The use of a single multimode fiber alone, without any special fabrication, as a sensor based on the light intensity variations is not an easy task. The twist effect on multimode fiber is used as an example herein. Experimental results show that light intensity through the multimode fiber shows no direct relationship wit...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Tianting Zhong Zhipeng Yu Huanhao Li Zihao Li Haohong Li Puxiang Lai Source Type: research

Fast optical wavefront engineering for controlling light propagation in dynamic turbid media
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. While propagating inside the strongly scattering biological tissue, photons lose their incident directions beyond one transport mean free path (TMFP, [math]1 millimeter (mm)), which makes it challenging to achieve optical focusing or clear imaging deep inside tissue. By manipulating many degrees of the incident optical wavefront, the latest optical wavefront engineering (WFE) technology compensates the wavefront distortions caused by the scattering media and thus is toward breaking this physical limit, bringing bright perspective to many applicat...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Meiyun Xia Deyu Li Ling Wang Daifa Wang Source Type: research

Artificial intelligence-assisted light control and computational imaging through scattering media
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. Coherent optical control within or through scattering media via wavefront shaping has seen broad applications since its invention around 2007. Wavefront shaping is aimed at overcoming the strong scattering, featured by random interference, namely speckle patterns. This randomness occurs due to the refractive index inhomogeneity in complex media like biological tissue or the modal dispersion in multimode fiber, yet this randomness is actually deterministic and potentially can be time reversal or precompensated. Various wavefront shaping approaches...
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Shengfu Cheng Huanhao Li Yunqi Luo Yuanjin Zheng Puxiang Lai Source Type: research

Editorial: Introduction to the special issue on high-resolution optical focusing and imaging within or through thick scattering media
Journal of Innovative Optical Health Sciences,Volume 12, Issue 04, July 2019. (Source: Journal of Innovative Optical Health Sciences)
Source: Journal of Innovative Optical Health Sciences - August 1, 2019 Category: Biomedical Science Authors: Puxiang Lai YongKeun Park Source Type: research

Automated segmentation of intraretinal cystoid macular edema based on gaussian mixture model
Journal of Innovative Optical Health Sciences, Ahead of Print. We introduce a method based on Gaussian mixture model (GMM) clustering and level-set to automatically detect intraretina fluid on diabetic retinopathy (DR) from spectral domain optical coherence tomography (SD-OCT) images in this paper. First, each B-scan is segmented using GMM clustering. The original clustering results are refined using location and thickness information. Then, the spatial information among every consecutive five B-scans is used to search potential fluid. Finally, the improved level-set method is used to obtain the accurate boundaries. The hi...
Source: Journal of Innovative Optical Health Sciences - July 25, 2019 Category: Biomedical Science Authors: Jinghong Wu Sijie Niu Qiang Chen Wen Fan Songtao Yuan Dengwang Li Source Type: research

Implementation and application of FRET –FLIM technology
Journal of Innovative Optical Health Sciences, Ahead of Print. With the development of the new detection methods and the function of fluorescent molecule, researchers hope to further explore the internal mechanisms of organisms, monitor changes in the intracellular microenvironment, and dynamic processes of molecular interactions in cells. Fluorescence resonance energy transfer (FRET) describes the energy transfer process between donor fluorescent molecules and acceptor fluorescent molecules. It is an important means to detect protein –protein interactions and protein conformation changes in cells. Fluorescence lifet...
Source: Journal of Innovative Optical Health Sciences - July 25, 2019 Category: Biomedical Science Authors: Shiqi Wang Binlin Shen Sheng Ren Yihua Zhao Silu Zhang Junle Qu Liwei Liu Source Type: research

Automated segmentation of intraretinal cystoid macular edema based on gaussian mixture model
Journal of Innovative Optical Health Sciences, Ahead of Print. We introduce a method based on Gaussian mixture model (GMM) clustering and level-set to automatically detect intraretina fluid on diabetic retinopathy (DR) from spectral domain optical coherence tomography (SD-OCT) images in this paper. First, each B-scan is segmented using GMM clustering. The original clustering results are refined using location and thickness information. Then, the spatial information among every consecutive five B-scans is used to search potential fluid. Finally, the improved level-set method is used to obtain the accurate boundaries. The hi...
Source: Journal of Innovative Optical Health Sciences - July 25, 2019 Category: Biomedical Science Authors: Jinghong Wu Sijie Niu Qiang Chen Wen Fan Songtao Yuan Dengwang Li Source Type: research

Brief review on learning-based methods for optical tomography
Journal of Innovative Optical Health Sciences, Ahead of Print. Learning-based methods have been proved to perform well in a variety of areas in the biomedical field, such as biomedical image segmentation, and histopathological image analysis. Deep learning, as the most recently presented approach of learning-based methods, has attracted more and more attention. For instance, massive researches of deep learning methods for image reconstructions of computed tomography (CT) and magnetic resonance imaging (MRI) have been reported, indicating the great potential of deep learning for inverse problems. Optical technology-related ...
Source: Journal of Innovative Optical Health Sciences - July 22, 2019 Category: Biomedical Science Authors: Lin Zhang Guanglei Zhang Source Type: research

Aggregation-induced emission luminogen for in vivo three-photon fluorescence lifetime microscopic imaging
Journal of Innovative Optical Health Sciences, Ahead of Print. Compared with visible light, near-infrared (NIR) light has deeper penetration in biological tissues. Three-photon fluorescence microscopy (3PFM) can effectively utilize the NIR excitation to obtain high-contrast images in the deep tissue. However, the weak three-photon fluorescence signals may be not well presented in the traditional fluorescence intensity imaging mode. Fluorescence lifetime of certain probes is insensitive to the intensity of the excitation laser. Moreover, fluorescence lifetime imaging microscopy (FLIM) can detect weak signals by utilizing ti...
Source: Journal of Innovative Optical Health Sciences - July 10, 2019 Category: Biomedical Science Authors: Huwei Ni Zicong Xu Dongyu Li Ming Chen Ben Zhong Tang Jun Qian Source Type: research

Two-photon excitation fluorescence lifetime imaging microscopy: A promising diagnostic tool for digestive tract tumors
Journal of Innovative Optical Health Sciences, Ahead of Print. Digestive tract tumors account for 15% and 19.3% of the cancer incidence and deaths, respectively. Early detection of digestive tract tumors is crucial to the reduction of global cancer burden. Two-photon excitation fluorescence lifetime imaging microscopy (TP-FLIM) allows non-invasive, label-free, three-dimensional, high-resolution imaging of living tissues with not only histological but also biochemical characterization ability in both qualitative and quantitative way. Benefiting from these advantages, this technology is promising for clinical diagnosis of di...
Source: Journal of Innovative Optical Health Sciences - July 10, 2019 Category: Biomedical Science Authors: Hui Li Jia Yu Rongli Zhang Xi Li Wei Zheng Source Type: research

Aggregation-induced emission luminogen for in vivo three-photon fluorescence lifetime microscopic imaging
Journal of Innovative Optical Health Sciences, Ahead of Print. Compared with visible light, near-infrared (NIR) light has deeper penetration in biological tissues. Three-photon fluorescence microscopy (3PFM) can effectively utilize the NIR excitation to obtain high-contrast images in the deep tissue. However, the weak three-photon fluorescence signals may be not well presented in the traditional fluorescence intensity imaging mode. Fluorescence lifetime of certain probes is insensitive to the intensity of the excitation laser. Moreover, fluorescence lifetime imaging microscopy (FLIM) can detect weak signals by utilizing ti...
Source: Journal of Innovative Optical Health Sciences - July 10, 2019 Category: Biomedical Science Authors: Huwei Ni Zicong Xu Dongyu Li Ming Chen Ben Zhong Tang Jun Qian Source Type: research

Recent advances in fluorescence-based in vivo flow cytometry
Journal of Innovative Optical Health Sciences, Ahead of Print. The fluorescence-based in vivo flow cytometry (IVFC) is an emerging tool to monitor circulating cells in vivo. As a noninvasive and real-time diagnostic technology, the fluorescence-based IVFC allows long-term monitoring of circulating cells without changing their native biological environment. It has been applied for various biological applications (e.g., monitoring circulating tumor cells). In this work, we will review our recent works on fluorescence-based IVFC. The operation principle and typical biological applications will be introduced. In addition, the ...
Source: Journal of Innovative Optical Health Sciences - July 2, 2019 Category: Biomedical Science Authors: Kai Pang Bobo Gu Feng Liu Mingli Dong Lianqing Zhu Xunbin Wei Source Type: research

Real-time detection of LMP1/LMP1 interaction in M[math]CD-induced apoptosis of nasopharyngeal carcinoma cells using FRET method
In this study, we revealed that M[math]CD induced apoptosis in two kinds of NPC cells including CNE1 cells, a LMP1-negative nasopharyngeal squamous carcinoma cell line, and CNE1-LMP1 cells, a LMP1-positive nasopharyngeal squamous carcinoma cell line. Furthermore, the impact of M[math]CD on LMP1 was investigated by fluorescence resonance energy transfer (FRET) method in NPC cells. Synchronized tempo-spatial and spectral detection of LMP1/LMP1 interaction were performed using fluorescence microscope and spectrograph. FRET efficiency indicated that LMP1/LMP1 interaction gradually enhanced after M[math]CD treatment. MTT assays...
Source: Journal of Innovative Optical Health Sciences - June 27, 2019 Category: Biomedical Science Authors: Zhiwei Wu Qing Ye Yimei Huang Yating Lin Mengmeng Zheng Xiaoyan Wang Shusen Xie Juqiang Lin Source Type: research

Exploiting the point spread function for optical imaging through a scattering medium based on deconvolution method
Journal of Innovative Optical Health Sciences, Ahead of Print. Visual perception of humans penetrating turbid medium is hampered by scattering. Various techniques have been prompted recently to recover optical imaging through turbid materials. Among them, speckle correlation based on deconvolution is one of the most attractive methods taking advantage of high imaging quality, robustness, ease-of-use, and ease-of-integration. By exploiting the point spread function (PSF) of the scattering system, large Field-of-View, extended Depth-of-Field, noninvasiveness and spectral resoluation are now available as successful solutions ...
Source: Journal of Innovative Optical Health Sciences - June 19, 2019 Category: Biomedical Science Authors: Hexiang He Xiangsheng Xie Yikun Liu Haowen Liang Jianying Zhou Source Type: research

Equidistant combination wavelength screening and step-by-step phase-out method for the near-infrared spectroscopic analysis of serum urea nitrogen
Journal of Innovative Optical Health Sciences, Ahead of Print. We applied near-infrared (NIR) spectroscopy with chemometrics for the rapid and reagent-free analysis of serum urea nitrogen (SUN). The modeling is based on the average effect of multiple sample partitions to achieve parameter selection with stability. A multiparameter optimization platform with Norris derivative filter –partial least squares (Norris-PLS) was developed to select the most suitable mode [math], [math], [math]. Using equidistant combination PLS (EC-PLS) with four parameters (initial wavelength [math], number of wavelengths [math], number of ...
Source: Journal of Innovative Optical Health Sciences - June 14, 2019 Category: Biomedical Science Authors: Yihui Yang Fenfen Lei Jing Zhang Lijun Yao Jiemei Chen Tao Pan Source Type: research

Fluorescence lifetime imaging microscopy and its applications in skin cancer diagnosis
Journal of Innovative Optical Health Sciences, Ahead of Print. Fluorescence lifetime (FLT) of fluorophores is sensitive to the changes in their surrounding microenvironment, and hence it can quantitatively reveal the physiological characterization of the tissue under investigation. Fluorescence lifetime imaging microscopy (FLIM) provides not only morphological but also functional information of the tissue by producing spatially resolved image of fluorophore lifetime, which can be used as a signature of disorder and/or malignancy in diseased tissues. In this paper, we begin by introducing the basic principle and common dete...
Source: Journal of Innovative Optical Health Sciences - June 14, 2019 Category: Biomedical Science Authors: Lixin Liu Qianqian Yang Meiling Zhang Zhaoqing Wu Ping Xue Source Type: research

Equidistant combination wavelength screening and step-by-step phase-out method for the near-infrared spectroscopic analysis of serum urea nitrogen
Journal of Innovative Optical Health Sciences, Ahead of Print. We applied near-infrared (NIR) spectroscopy with chemometrics for the rapid and reagent-free analysis of serum urea nitrogen (SUN). The modeling is based on the average effect of multiple sample partitions to achieve parameter selection with stability. A multiparameter optimization platform with Norris derivative filter –partial least squares (Norris-PLS) was developed to select the most suitable mode [math], [math], [math]. Using equidistant combination PLS (EC-PLS) with four parameters (initial wavelength [math], number of wavelengths [math], number of ...
Source: Journal of Innovative Optical Health Sciences - June 14, 2019 Category: Biomedical Science Authors: Yihui Yang Fenfen Lei Jing Zhang Lijun Yao Jiemei Chen Tao Pan Source Type: research