Three-dimensional cell culture can be regulated by vibration: low-frequency vibration increases the size of olfactory ensheathing cell spheroids
Olfactory ensheathing cell (OEC) transplantation is emerging as a promising therapy for spinal cord injuries. However, outcomes are inconsistent, and the method needs improvement. Currently, cells are injected... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - May 16, 2019 Category: Biotechnology Authors: Lachlan J. Beckingham, Michael Todorovic, Johana Tello Velasquez, Marie-Laure Vial, Mo Chen, Jenny A. K. Ekberg and James A. St John Tags: Research Source Type: research
Efficient reduction of synthetic mRNA induced immune activation by simultaneous delivery of B18R encoding mRNA
The application of synthetic modified messenger RNA (mRNA) is a promising approach for the treatment of a variety of diseases and vaccination. In the past few years, different modifications of synthetic mRNA w... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - May 9, 2019 Category: Biotechnology Authors: Tatjana Michel, Sonia Golombek, Heidrun Steinle, Ludmilla Hann, Ana Velic, Boris Macek, Stefanie Krajewski, Christian Schlensak, Hans Peter Wendel and Meltem Avci-Adali Tags: Research Source Type: research
Electrical energy storage with engineered biological systems
The availability of renewable energy technologies is increasing dramatically across the globe thanks to their growing maturity. However, large scale electrical energy storage and retrieval will almost certainl... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - May 3, 2019 Category: Biotechnology Authors: Farshid Salimijazi, Erika Parra and Buz Barstow Tags: Review Source Type: research
Genetic circuits to engineer tissues with alternative functions
Persistent and complex problems arising with respect to human physiology and pathology have led to intense investigation into therapies and tools that permit more targeted outcomes and biomimetic responses to ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - May 3, 2019 Category: Biotechnology Authors: C. P. Healy and T. L. Deans Tags: Review Source Type: research
Publisher Correction to: delivery systems of CRISPR/Cas9-based cancer gene therapy
In the original version of this article [1], published on 18 December 2018, there was 1 incorrect author name. (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - May 2, 2019 Category: Biotechnology Authors: Alessio Biagioni, Anna Laurenzana, Francesca Margheri, Anastasia Chill à, Gabriella Fibbi and Mario Del Rosso Tags: Publisher Correction Source Type: research
Molecular transport through primary human small intestinal monolayers by culture on a collagen scaffold with a gradient of chemical cross-linking
The luminal surface of the small intestine is composed of a monolayer of cells overlying a lamina propria comprised of extracellular matrix (ECM) proteins. The ECM provides a porous substrate critical for nutrien... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - April 27, 2019 Category: Biotechnology Authors: Jennifer E. Speer, Dulan B. Gunasekara, Yuli Wang, John K. Fallon, Peter J. Attayek, Philip C. Smith, Christopher E. Sims and Nancy L. Allbritton Tags: Research Source Type: research
krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells
CRISPR/Cas9 system is a powerful tool for knocking out genes in cells. However, genes essential for cell survival cannot be directly knocked out. Traditionally, generation of conditional knockout cells require... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - April 24, 2019 Category: Biotechnology Authors: Bei Wang, Zishi Wang, Daqi Wang, Baolong Zhang, Sang-Ging Ong, Mingqing Li, Wenqiang Yu and Yongming Wang Tags: Research Source Type: research
Quantitative study of H protein lipoylation of the glycine cleavage system and a strategy to increase its activity by co-expression of LplA
Glycine cleavage system (GCS) plays a key role in one-carbon (C1) metabolism related to the biosynthesis of a number of key intermediates with significance in both biomedicine and biotechnology. Despite extens... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - April 23, 2019 Category: Biotechnology Authors: Xinyi Zhang, Mei Li, Yingying Xu, Jie Ren and An-Ping Zeng Tags: Research Source Type: research
Tools to reverse-engineer multicellular systems: case studies using the fruit fly
Reverse-engineering how complex multicellular systems develop and function is a grand challenge for systems bioengineers. This challenge has motivated the creation of a suite of bioengineering tools to develop... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - April 23, 2019 Category: Biotechnology Authors: Qinfeng Wu, Nilay Kumar, Vijay Velagala and Jeremiah J. Zartman Tags: Review Source Type: research
Novel biomanufacturing platform for large-scale and high-quality human T cells production
The objective of this study was ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - April 23, 2019 Category: Biotechnology Authors: Jianfa Ou, Yingnan Si, Yawen Tang, Grace E. Salzer, Yun Lu, Seulhee Kim, Hongwei Qin, Lufang Zhou and Xiaoguang Liu Tags: Research Source Type: research
A simple approach for rapid and cost-effective quantification of extracellular vesicles using a fluorescence polarization technique
Extracellular vesicles (EVs) are membrane-bound phospholipid vesicles actively secreted by all cells. As they carry specific markers expressed by their parental cells, EVs are utilized to identify specific cel... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - April 16, 2019 Category: Biotechnology Authors: Kalishwaralal Kalimuthu, Woo Young Kwon and Ki Soo Park Tags: Research Source Type: research
Systematic analysis of the effects of different nitrogen source and ICDH knockout on glycolate synthesis in Escherichia coli
Glycolate is an important α-hydroxy carboxylic acid widely used in industrial and consumer applications. The production of glycolate from glucose in Escherichia coli is generally carried out by glycolysis and gly... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - April 4, 2019 Category: Biotechnology Authors: Kangjia Zhu, Guohui Li, Ren Wei, Yin Mao, Yunying Zhao, Aiyong He, Zhonghu Bai and Yu Deng Tags: Research Source Type: research
Three-dimensional microengineered models of human cardiac diseases
In vitro three-dimensional (3D) microengineered tissue models have been the recent focus of pathophysiological studies, particularly in the field of cardiovascular research. These models, as classified by 3D b... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - April 3, 2019 Category: Biotechnology Authors: Jaimeson Veldhuizen, Raymond Q. Migrino and Mehdi Nikkhah Tags: Review Source Type: research
Microvascular bioengineering: a focus on pericytes
Capillaries within the microcirculation are essential for oxygen delivery and nutrient/waste exchange, among other critical functions. Microvascular bioengineering approaches have sought to recapitulate many k... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - March 29, 2019 Category: Biotechnology Authors: Huaning Zhao and John C. Chappell Tags: Review Source Type: research
Analysis of the stability and affinity of BlaR-CTD protein to β-lactam antibiotics based on docking and mutagenesis studies
Owing to the thermal instability and low affinity of BlaR-CTD to some β-lactams, the receptor assay based on BlaR-CTD is limited in the detection of abundant variety of drugs and the result is often unstable. ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - March 29, 2019 Category: Biotechnology Authors: Jianan Ning, Saeed Ahmed, Guyue Cheng, Ting Chen, Yulian Wang, Dapeng Peng and Zonghui Yuan Tags: Research Source Type: research
