A novel approach for T7 bacteriophage genome integration of exogenous DNA
The comparatively small genome, well elucidated functional genomics and rapid life cycle confer T7 bacteriophage with great advantages for bio-application. Genetic manipulation of T7 genome plays a key role in... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - January 16, 2020 Category: Biotechnology Authors: Ying Liu, Hongxing Huang, Hua Wang and Yan Zhang Tags: Research Source Type: research

SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding
Aptamers, single-stranded DNAs or RNAs, can be selected from a library containing random sequences using a method called Systematic Evolution of Ligands by EXponential Enrichment (SELEX). In SELEX, monitoring ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - January 13, 2020 Category: Biotechnology Authors: Qingxiu Liu, Wei Zhang, Siying Chen, Zhenjing Zhuang, Yi Zhang, Lingli Jiang and Jun Sheng LIN Tags: Research Source Type: research

Super-antibiofilm effect of N2 plasma treated buffer (NPB) against plant pathogenic bacterium
Controlling of biofilms formation in numerous pathogenic bacteria is one of the most difficult tasks in the control of bacterial diseases. Plasma has attracted extensive attention due to their potential applic... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - December 4, 2019 Category: Biotechnology Authors: Hyemi Seo, Jisoo Hong, Taeyeol Kim, Won ll Choi, Daekyung Sung and Eunpyo Moon Tags: Research Source Type: research

CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli
Synthetic biology heavily depends on rapid and simple techniques for DNA engineering, such as Ligase Cycling Reaction (LCR), Gibson assembly and Golden Gate assembly, all of which allow for fast, multi-fragmen... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 28, 2019 Category: Biotechnology Authors: Stefano Vecchione and Georg Fritz Tags: Methodology Source Type: research

Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
This paper introduces a new technique combining microdialysis with patch clamp to detect the changes in ATP (adenosine triphosphate) concentration in acupoints during acupuncture. The microdialysis probe was i... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 28, 2019 Category: Biotechnology Authors: Yong Wu, Meng Huang, Ying Xia and Guanghong Ding Tags: Research Source Type: research

Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
Transcription factor-based biosensors are useful tools for the detection of metabolites and industrially valuable molecules, and present many potential applications in biotechnology and biomedicine. However, t... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 27, 2019 Category: Biotechnology Authors: Leopoldo F. M. Machado, Andrew Currin and Neil Dixon Tags: Research Source Type: research

High capacity DNA data storage with variable-length Oligonucleotides using repeat accumulate code and hybrid mapping
With the inherent high density and durable preservation, DNA has been recently recognized as a distinguished medium to store enormous data over millennia. To overcome the limitations existing in a recently rep... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 21, 2019 Category: Biotechnology Authors: Yixin Wang, Md Noor-A-Rahim, Jingyun Zhang, Erry Gunawan, Yong Liang Guan and Chueh Loo Poh Tags: Research Source Type: research

HMEJ-mediated efficient site-specific gene integration in chicken cells
The production of transgenic chicken cells holds great promise for several diverse areas, including developmental biology and biomedical research. To this end, site-specific gene integration has been an attrac... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 21, 2019 Category: Biotechnology Authors: Long Xie, Juanjuan Sun, Lifen Mo, Tianpeng Xu, Qaisar Shahzad, Dongyang Chen, Wenhao Yang, Yuying Liao and Yangqing Lu Tags: Research Source Type: research

Identification of urban particulate matter-induced disruption of human respiratory mucosa integrity using whole transcriptome analysis and organ-on-a chip
Exposure to air particulate matter (PM) is associated with various diseases in the human respiratory system. To date, most in vitro studies showing cellular responses to PM have been performed in cell culture ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 15, 2019 Category: Biotechnology Authors: Junhyoung Byun, Boa Song, Kyungwoo Lee, Byoungjae Kim, Hae Won Hwang, Myung-Ryul Ok, Hojeong Jeon, Kijeong Lee, Seung-Kuk Baek, Sang-Heon Kim, Seung Ja Oh and Tae Hoon Kim Tags: Research Source Type: research

Antigen-labeled mesoporous silica-coated Au-core Pt-shell nanostructure: a novel nanoprobe for highly efficient virus diagnosis
As an emerging research area of artificial enzymes, nanozyme, the catalytic nanomaterials with enzyme-like characteristics, have attracted enormous attention in research. Here, a nanozyme probe has been realiz... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 14, 2019 Category: Biotechnology Authors: Aiyun Li, Lin Long, Fengshou Liu, Jianbo Liu, Xiaochun Wu and Yinglu Ji Tags: Research Source Type: research

An overview of advanced biocompatible and biomimetic materials for creation of replacement structures in the musculoskeletal systems: focusing on cartilage tissue engineering
Tissue engineering, as an interdisciplinary approach, is seeking to create tissues with optimal performance for clinical applications. Various factors, including cells, biomaterials, cell or tissue culture con... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 13, 2019 Category: Biotechnology Authors: Azizeh Rahmani Del Bakhshayesh, Nahideh Asadi, Alireza Alihemmati, Hamid Tayefi Nasrabadi, Azadeh Montaseri, Soodabeh Davaran, Sepideh Saghati, Abolfazl Akbarzadeh and Ali Abedelahi Tags: Review Source Type: research

Effects of endogenous inflammation signals elicited by nerve growth factor, interferon- γ, and interleukin-4 on peripheral nerve regeneration
Large gap healing is a difficult issue in the recovery of peripheral nerve injury. The present study provides in vivo trials of silicone rubber chambers filled with collagen containing IFN- γ or IL-4 to bridge ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 13, 2019 Category: Biotechnology Authors: Chien-Fu Liao, Chung-Chia Chen, Yu-Wen Lu, Chun-Hsu Yao, Jia-Horng Lin, Tzong-Der Way, Tse-Yen Yang and Yueh-Sheng Chen Tags: Research Source Type: research

Electrospun nanofibers for the fabrication of engineered vascular grafts
Attention has recently increased in the application of electrospun fibers because of their putative capability to create nanoscale platforms toward tissue engineering. To some extent, electrospun fibers are ap... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 11, 2019 Category: Biotechnology Authors: Sonia Fathi Karkan, Soodabeh Davaran, Reza Rahbarghazi, Roya Salehi and Abolfazl Akbarzadeh Tags: Review Source Type: research

Correction to: Computational analysis of viable parameter regions in models of synthetic biological systems
The original version of this article [1] unfortunately included a typographical error to author Žiga Pušnik’s name. (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 11, 2019 Category: Biotechnology Authors: Žiga Pušnik, Miha Mraz, Nikolaj Zimic and Miha Moškon Tags: Correction Source Type: research

Evaluation of human primary intestinal monolayers for drug metabolizing capabilities
The intestinal epithelium is a major site of drug metabolism in the human body, possessing enterocytes that house brush border enzymes and phase I and II drug metabolizing enzymes (DMEs). The enterocytes are s... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 4, 2019 Category: Biotechnology Authors: Jennifer E. Speer, Yuli Wang, John K. Fallon, Philip C. Smith and Nancy L. Allbritton Tags: Research Source Type: research

Unique N-glycosylation of a recombinant exo-inulinase from Kluyveromyces cicerisporus and its effect on enzymatic activity and thermostability
Inulinase can hydrolyze polyfructan into high-fructose syrups and fructoligosaccharides, which are widely used in food, the medical industry and the biorefinery of Jerusalem artichoke. In the present study, a rec... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - October 29, 2019 Category: Biotechnology Authors: Junyan Ma, Qian Li, Haidong Tan, Hao Jiang, Kuikui Li, Lihua Zhang, Quan Shi and Heng Yin Tags: Research Source Type: research

3D-tips: user-friendly mesh barrier pipette tips for 3D-spheroid culture
Three dimensional (3D) cell cultures have been an area of increasing interest and relevance across several research fields including drug discovery, developmental biology and stem cell-based therapies. However... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - October 28, 2019 Category: Biotechnology Authors: Mo Chen, Marie-Laure Vial and James A. St John Tags: Research Source Type: research

A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering
The main issue in cutaneous regeneration is to develop engineered scaffolds based on natural extracellular matrix to promote dynamics of skin progenitor cells and accelerate differentiation into mature keratin... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - October 26, 2019 Category: Biotechnology Authors: Leila Mohammadzadeh, Reza Rahbarghazi, Roya Salehi and Mehrdad Mahkam Tags: Research Source Type: research

Process development for pandemic influenza VLP vaccine production using a baculovirus expression system
Influenza viruses cause hundreds of thousands of respiratory diseases worldwide each year, and vaccination is considered the most effective approach for preventing influenza annual epidemics or pandemics. Sinc... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - October 23, 2019 Category: Biotechnology Authors: Chia-Chun Lai, Yu-Chieh Cheng, Pin-Wen Chen, Ting-Hui Lin, Tsai-Teng Tzeng, Chia-Chun Lu, Min-Shi Lee and Alan Yung-Chih Hu Tags: Research Source Type: research

Recombinant baculovirus expressing the FrC-OVA protein induces protective antitumor immunity in an EG7-OVA mouse model
The baculovirus (BV) Autographa californica multiple nuclear polyhedrosis virus has been used in numerous protein expression systems because of its ability to infect insect cells and serves as a useful vaccinatio... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - October 22, 2019 Category: Biotechnology Authors: Keigo Kondou, Tomoyuki Suzuki, Myint Oo Chang and Hiroshi Takaku Tags: Research Source Type: research

De Novo Isolation & Affinity Maturation of yeast-displayed Virion-binding human fibronectin domains by flow cytometric screening against Virions
The promise of biopharmaceuticals comprising one or more binding domains motivates the development of novel methods for de novo isolation and affinity maturation of virion-binding domains. Identifying avenues ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - October 16, 2019 Category: Biotechnology Authors: Pete Heinzelman, Alyssa Low, Rudo Simeon, Gus A. Wright and Zhilei Chen Tags: Research Source Type: research

Computational analysis of viable parameter regions in models of synthetic biological systems
Gene regulatory networks with different topological and/or dynamical properties might exhibit similar behavior. System that is less perceptive for the perturbations of its internal and external factors should ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - September 18, 2019 Category: Biotechnology Authors: žiga Pušnik, Miha Mraz, Nikolaj Zimic and Miha Moškon Tags: Methodology Source Type: research

Strategies for the expansion of human induced pluripotent stem cells as aggregates in single-use Vertical-Wheel ™ bioreactors
Since their inception, human induced pluripotent stem cells (hiPSCs) have held much promise for pharmacological applications and cell-based therapies. However, their potential can only be realised if large num... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - September 14, 2019 Category: Biotechnology Authors: Diogo E. S. Nogueira, Carlos A. V. Rodrigues, Marta S. Carvalho, Cl áudia C. Miranda, Yas Hashimura, Sunghoon Jung, Brian Lee and Joaquim M. S. Cabral Tags: Research Source Type: research

Jamming state transition and collective cell migration
Jamming state transition has been used in literature to describe migrating-to-resting cell state transition during collective cell migration without proper rheological confirmation. Yield stress often has been... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - September 5, 2019 Category: Biotechnology Authors: Ivana Pajic-Lijakovic and Milan Milivojevic Tags: Research Source Type: research

Marimo machines: oscillators, biosensors and actuators
The green algae balls (Aegagropila linnaei), known as Marimo, are large spherical colonies of live photosynthetic filaments, formed by rolling water currents in freshwater lakes. Photosynthesis therein produces g... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - September 3, 2019 Category: Biotechnology Authors: Neil Phillips, Thomas C. Draper, Richard Mayne and Andrew Adamatzky Tags: Research Source Type: research

Exosomes derived from microRNA-138-5p-overexpressing bone marrow-derived mesenchymal stem cells confer neuroprotection to astrocytes following ischemic stroke via inhibition of LCN2
MicroRNAs (miRNAs) are implicated in the progression of ischemic stroke (IS) and bone marrow-derived mesenchymal stem cells (BMSCs)-derived exosomes play a role in IS therapy. Herein we hypothesized that the B... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - August 28, 2019 Category: Biotechnology Authors: Yiming Deng, Duanduan Chen, Feng Gao, Hong Lv, Guojun Zhang, Xuan Sun, Lian Liu, Dapeng Mo, Ning Ma, Ligang Song, Xiaochuan Huo, Tianyi Yan, Jingbo Zhang and Zhongrong Miao Tags: Research Source Type: research

Using the promoters of MerR family proteins as “rheostats” to engineer whole-cell heavy metal biosensors with adjustable sensitivity
Whole cell biosensors provide a simple method for the detection of heavy metals. However, previous designs of them rely primarily on simulation of heavy metal resistance systems of bacteria. (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - August 20, 2019 Category: Biotechnology Authors: Mingzhang Guo, Ruoxi Du, Zixin Xie, Xiaoyun He, Kunlun Huang, Yunbo Luo and Wentao Xu Tags: Research Source Type: research

Targeting CYP4A attenuates hepatic steatosis in a novel multicellular organotypic liver model
Non-alcoholic fatty liver disease (NAFLD) begins as simple hepatic steatosis, but further progress to chronic liver diseases results in severe liver damage and hepatic failure. However, therapeutic options are... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - August 8, 2019 Category: Biotechnology Authors: Jae-Sung Ryu, Minji Lee, Seon Ju Mun, Sin-Hyoung Hong, Ho-Joon Lee, Hyo-Suk Ahn, Kyung-Sook Chung, Gun-Hwa Kim and Myung Jin Son Tags: Research Source Type: research

The LINC complex, mechanotransduction, and mesenchymal stem cell function and fate
Mesenchymal stem cells (MSCs) show tremendous promise as a cell source for tissue engineering and regenerative medicine, and are understood to be mechanosensitive to external mechanical environments. In recent... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - August 7, 2019 Category: Biotechnology Authors: Tasneem Bouzid, Eunju Kim, Brandon D. Riehl, Amir Monemian Esfahani, Jordan Rosenbohm, Ruiguo Yang, Bin Duan and Jung Yul Lim Tags: Review Source Type: research

Discovery and engineering of an endophytic Pseudomonas strain from Taxus chinensis for efficient production of zeaxanthin diglucoside
Endophytic microorganisms are a rich source of bioactive natural products. They are considered as promising biofertilizers and biocontrol agents due to their growth-promoting interactions with the host plants ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - August 1, 2019 Category: Biotechnology Authors: Ozkan Fidan and Jixun Zhan Tags: Research Source Type: research

Neuromodulation of metabolic functions: from pharmaceuticals to bioelectronics to biocircuits
Neuromodulation of central and peripheral neural circuitry brings together neurobiologists and neural engineers to develop advanced neural interfaces to decode and recapitulate the information encoded in the n... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - August 1, 2019 Category: Biotechnology Authors: Benjamin J. Seicol, Sebastian Bejarano, Nicholas Behnke and Liang Guo Tags: Review Source Type: research

Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase
Creating designer molecules using a combination of select domains from polyketide synthases and/or nonribosomal peptide synthetases (NRPS) continues to be a synthetic goal. However, an incomplete understanding... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - July 31, 2019 Category: Biotechnology Authors: Fuchao Xu, Russell Butler, Kyle May, Megi Rexhepaj, Dayu Yu, Jiachen Zi, Yi Chen, Yonghong Liang, Jia Zeng, Joan Hevel and Jixun Zhan Tags: Research Source Type: research

Performance evaluation of a hybrid sequencing batch reactor under saline and hyper saline conditions
Significant rise in concentration of saline wastewater entering the treatment plants has been resulting in many problems in the biological treatment processes. On the other hand, the specific conditions of phy... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - July 29, 2019 Category: Biotechnology Authors: Mostafa Tizghadam Ghazani and Alireza Taghdisian Tags: Research Source Type: research

The engineering principles of combining a transcriptional incoherent feedforward loop with negative feedback
Regulation of gene expression is of paramount importance in all living systems. In the past two decades, it has been discovered that certain motifs, such as the feedforward motif, are overrepresented in gene r... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - July 10, 2019 Category: Biotechnology Authors: Gregory T. Reeves Tags: Research Source Type: research

Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates
An adapted strategy from the conventional 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) crosslinking method was developed to form a covalently coupled phosphoramidated single stranded DNA ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - July 10, 2019 Category: Biotechnology Authors: Malithi P. Wickramathilaka and Bernard Y. Tao Tags: Research Source Type: research

A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities
Silk glands are used by silkworms to spin silk fibers for making their cocoons. These have recently been regarded as bioreactor hosts for the cost-effective production of other valuable exogenous proteins and ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - July 5, 2019 Category: Biotechnology Authors: Sheng Xu, Feng Wang, Yuancheng Wang, Riyuan Wang, Kai Hou, Chi Tian, Yanting Ji, Qianqian Yang, Ping Zhao and Qingyou Xia Tags: Research Source Type: research

Long non-coding RNA PVT1 knockdown suppresses fibroblast-like synoviocyte inflammation and induces apoptosis in rheumatoid arthritis through demethylation of sirt6
As a type of chronic autoimmune joint disease, rheumatoid arthritis (RA) is a disorder, characterized by a variety of physical symptoms as well as RA fibroblast-like synoviocyte (RA-FLS) proliferation. More re... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - July 2, 2019 Category: Biotechnology Authors: Chun-Wang Zhang, Xia Wu, Dan Liu, Wei Zhou, Wei Tan, Yu-Xuan Fang, Yu Zhang, Yan-Qing Liu and Guo-Qing Li Tags: Research Source Type: research

Cardiac tissue engineering: state-of-the-art methods and outlook
The purpose of this review is to assess the state-of-the-art fabrication methods, advances in genome editing, and the use of machine learning to shape the prospective growth in cardiac tissue engineering. Thos... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - June 28, 2019 Category: Biotechnology Authors: Anh H. Nguyen, Paul Marsh, Lauren Schmiess-Heine, Peter J. Burke, Abraham Lee, Juhyun Lee and Hung Cao Tags: Review Source Type: research

Intestinal probiotics E. coli Nissle 1917 as a targeted vehicle for delivery of p53 and Tum-5 to solid tumors for cancer therapy
Traditional cancer therapies, such as surgery treatment, radiotherapy, and chemotherapy, often fail to completely eliminate tumor cells in an anaerobic microenvironment of tumor regions. (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - June 28, 2019 Category: Biotechnology Authors: Lian He, Huijun Yang, Jianli Tang, Zhudong Liu, Yiyan Chen, Binghua Lu, Haocheng He, Sijia Tang, Yunjun Sun, Fei Liu, Xuezhi Ding, Youming Zhang, Shengbiao Hu and Liqiu Xia Tags: Research Source Type: research

Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells
The production of recombinant proteins in mammalian cell lines is one of the most important areas in biopharmaceutical industry. Viral transcriptional promoters are widely used to express recombinant proteins ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - June 28, 2019 Category: Biotechnology Authors: Roberto A Z úñiga, Matías Gutiérrez-González, Norberto Collazo, Pablo Hérnan Sotelo, Carolina H Ribeiro, Claudia Altamirano, Carmen Lorenzo, Juan Carlos Aguillón and María Carmen Molina Tags: Research Source Type: research

Construction of an immunotoxin via site-specific conjugation of anti-Her2 IgG and engineered Pseudomonas exotoxin A
Immunotoxins consisting of a toxin from bacteria or plants and a targeting module have been developed as potent anti-cancer therapeutics. The majority of them, especially those in preclinical or clinical testi... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - June 21, 2019 Category: Biotechnology Authors: Byeong Sung Lee, Yumi Lee, Jisoo Park, Bo Seok Jeong, Migyeong Jo, Sang Taek Jung and Tae Hyeon Yoo Tags: Research Source Type: research

Functional cellulose-based hydrogels as extracellular matrices for tissue engineering
Cellulose-based hydrogels are immensely important for tissue engineering. In this review, we attempt to document the source, nature, and application of cellulose-based hydrogels as an extracellular matrix for ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - June 20, 2019 Category: Biotechnology Authors: Sayan Deb Dutta, Dinesh K. Patel and Ki-Taek Lim Tags: Review Source Type: research

Engineered mosaic protein polymers; a simple route to multifunctional biomaterials
Engineered living materials (ELMs) are an exciting new frontier, where living organisms create highly functional materials. In particular, protein ELMs have the advantage that their properties can be manipulat... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - June 18, 2019 Category: Biotechnology Authors: Daniel T. Peters, Helen Waller, Mark A. Birch and Jeremy H. Lakey Tags: Research Source Type: research

Rise of the killer plants: investigating the antimicrobial activity of Australian plants to enhance biofilter-mediated pathogen removal
Biofilters are soil-plant based passive stormwater treatment systems which demonstrate promising, although inconsistent, removal of faecal microorganisms. Antimicrobial-producing plants represent a safe, inexp... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - June 6, 2019 Category: Biotechnology Authors: P. Galbraith, R. Henry and D. T. McCarthy Tags: Research Source Type: research

Contribution of nonconsensus base pairs within ArsR binding sequences toward ArsR-DNA binding and arsenic-mediated transcriptional induction
A transcriptional reporter is the key component in bacterial biosensors which are employed to monitor the induction or repression of a reporter gene corresponding to environmental change. Interaction of a tran... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - June 6, 2019 Category: Biotechnology Authors: Xingjuan Chen, Xin Jiang, Cuijuan Tie, Jinnon Yoo, Yan Wang, Meiying Xu, Guoping Sun, Jun Guo and Xianqiang Li Tags: Research Source Type: research

Label-free and washing-free alkaline phosphatase assay using a personal glucose meter
We herein describe a personal glucose meter (PGM)-based method for a label-free and washing-free determination of alkaline phosphatase (ALP) activity, which relies on the cascade enzymatic reactions promoted b... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - June 4, 2019 Category: Biotechnology Authors: Jun Ki Ahn, Hyo Yong Kim, Chang Yeol Lee, Ki Soo Park and Hyun Gyu Park Tags: Letter to the Editor Source Type: research

Expression profile analysis of long non-coding RNA in skeletal muscle of osteoporosis by microarray and bioinformatics
Osteoporosis (OP) is a condition featured by bone mass loss and bone tissue microarchitectural alterations due to impaired tissue homeostasis favoring excessive bone resorption versus deposition. The trigger o... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - May 31, 2019 Category: Biotechnology Authors: Shaojin Liu, Hongxing Huang, Shuang Chai, Hewei Wei, Jiachun Huang and Lei Wan Tags: Research Source Type: research

Transplantation of CRISPRa system engineered IL10-overexpressing bone marrow-derived mesenchymal stem cells for the treatment of myocardial infarction in diabetic mice
Myocardial infarction (MI) is a common cause of mortality in people. Mesenchymal stem cell (MSC) has been shown to exert therapeutic potential to treat myocardial infarction (MI). However, in patients with dia... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - May 30, 2019 Category: Biotechnology Authors: Xin Meng, Minjuan Zheng, Ming Yu, Wei Bai, Lei Zuo, Xin Bu, Yi Liu, Linying Xia, Jing Hu, Liwen Liu and Jianping Li Tags: Research Source Type: research

Mixture theory modeling for characterizing solute transport in breast tumor tissues
Tumor numerical models have been used to quantify solute transport with a single capillary embedded in an infinite tumor expanse, but measurements from different mammalian tumors suggest that a tissue containi... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - May 29, 2019 Category: Biotechnology Authors: Sreyashi Chakraborty, Alican Ozkan, Marissa Nichole Rylander, Wendy A. Woodward and Pavlos Vlachos Tags: Research Source Type: research

Long-term continuous treatment of non-sterile real hospital wastewater by Trametes versicolor
Hospital wastewater is commonly polluted with high loads of pharmaceutically active compounds, which pass through wastewater treatment plants (WWTPs) and end up in water bodies, posing ecological and health ri... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - May 29, 2019 Category: Biotechnology Authors: Josep Anton Mir-Tutusaus, Eloi Parlad é, Marta Villagrasa, Damià Barceló, Sara Rodríguez-Mozaz, Maira Martínez-Alonso, Núria Gaju, Montserrat Sarrà and Glòria Caminal Tags: Research Source Type: research