Design and testing of a centrifugal fluidic device for populating microarrays of spheroid cancer cell cultures
In current cancer spheroid culturing methods, the transfer and histological processing of specimens grown in 96-well plates is a time consuming process. A centrifugal fluidic device was developed and tested fo... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - March 12, 2020 Category: Biotechnology Authors: Warren Weisler, Samuel Miller, Shaphan Jernigan, Gregory Buckner and Matthew Bryant Tags: Research Source Type: research
Heparinized chitosan stabilizes the bioactivity of BMP-2 and potentiates the osteogenic efficacy of demineralized bone matrix
Demineralized bone matrix (DBM), an allograft bone processed to better expose osteoinductive factors such as bone morphogenetic proteins (BMPs), is increasingly used for clinical bone repair. However, more ext... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - March 6, 2020 Category: Biotechnology Authors: Soyon Kim, Jiabing Fan, Chung-Sung Lee, Chen Chen, Ksenia Bubukina and Min Lee Tags: Research Source Type: research
Biodegradation of penicillin G from industrial bacteria residue by immobilized cells of Paracoccus sp. KDSPL-02 through continuous expanded bed adsorption bioreactor
An efficient biodegradation-strengthening approach was developed to improve penicillin G degradation from industrial bacterial residue in an expanded bed adsorption bioreactor (EBAB) is reported in this paper. (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - February 22, 2020 Category: Biotechnology Authors: Peng Wang, Chen Shen, Xiaochun Wang, Shouxin Liu, Luwei Li and Jinfeng Guo Tags: Research Source Type: research
Superoxide dismutase reduces monosodium glutamate-induced injury in an organotypic whole hemisphere brain slice model of excitotoxicity
Knowledge of glutamate excitotoxicity has increased substantially over the past few decades, with multiple proposed pathways involved in inflicting damage. We sought to develop a monosodium glutamate (MSG) exp... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - February 4, 2020 Category: Biotechnology Authors: Rick Liao, Thomas R. Wood and Elizabeth Nance Tags: Research Source Type: research
Signature mRNA markers in extracellular vesicles for the accurate diagnosis of colorectal cancer
With the increasing incidence of colorectal cancer (CRC), its accurate diagnosis is critical and in high demand. However, conventional methods are not ideal due to invasiveness and low accuracy. Herein, we aim... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - February 4, 2020 Category: Biotechnology Authors: Byung Seok Cha, Ki Soo Park and Jun Seok Park Tags: Research Source Type: research
A novel approach for T7 bacteriophage genome integration of exogenous DNA
The comparatively small genome, well elucidated functional genomics and rapid life cycle confer T7 bacteriophage with great advantages for bio-application. Genetic manipulation of T7 genome plays a key role in... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - January 16, 2020 Category: Biotechnology Authors: Ying Liu, Hongxing Huang, Hua Wang and Yan Zhang Tags: Research Source Type: research
SELEX tool: a novel and convenient gel-based diffusion method for monitoring of aptamer-target binding
Aptamers, single-stranded DNAs or RNAs, can be selected from a library containing random sequences using a method called Systematic Evolution of Ligands by EXponential Enrichment (SELEX). In SELEX, monitoring ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - January 13, 2020 Category: Biotechnology Authors: Qingxiu Liu, Wei Zhang, Siying Chen, Zhenjing Zhuang, Yi Zhang, Lingli Jiang and Jun Sheng LIN Tags: Research Source Type: research
Super-antibiofilm effect of N2 plasma treated buffer (NPB) against plant pathogenic bacterium
Controlling of biofilms formation in numerous pathogenic bacteria is one of the most difficult tasks in the control of bacterial diseases. Plasma has attracted extensive attention due to their potential applic... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - December 4, 2019 Category: Biotechnology Authors: Hyemi Seo, Jisoo Hong, Taeyeol Kim, Won ll Choi, Daekyung Sung and Eunpyo Moon Tags: Research Source Type: research
CRIMoClo plasmids for modular assembly and orthogonal chromosomal integration of synthetic circuits in Escherichia coli
Synthetic biology heavily depends on rapid and simple techniques for DNA engineering, such as Ligase Cycling Reaction (LCR), Gibson assembly and Golden Gate assembly, all of which allow for fast, multi-fragmen... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 28, 2019 Category: Biotechnology Authors: Stefano Vecchione and Georg Fritz Tags: Methodology Source Type: research
Real-time analysis of ATP concentration in acupoints during acupuncture: a new technique combining microdialysis with patch clamp
This paper introduces a new technique combining microdialysis with patch clamp to detect the changes in ATP (adenosine triphosphate) concentration in acupoints during acupuncture. The microdialysis probe was i... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 28, 2019 Category: Biotechnology Authors: Yong Wu, Meng Huang, Ying Xia and Guanghong Ding Tags: Research Source Type: research
Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes
Transcription factor-based biosensors are useful tools for the detection of metabolites and industrially valuable molecules, and present many potential applications in biotechnology and biomedicine. However, t... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 27, 2019 Category: Biotechnology Authors: Leopoldo F. M. Machado, Andrew Currin and Neil Dixon Tags: Research Source Type: research
High capacity DNA data storage with variable-length Oligonucleotides using repeat accumulate code and hybrid mapping
With the inherent high density and durable preservation, DNA has been recently recognized as a distinguished medium to store enormous data over millennia. To overcome the limitations existing in a recently rep... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 21, 2019 Category: Biotechnology Authors: Yixin Wang, Md Noor-A-Rahim, Jingyun Zhang, Erry Gunawan, Yong Liang Guan and Chueh Loo Poh Tags: Research Source Type: research
HMEJ-mediated efficient site-specific gene integration in chicken cells
The production of transgenic chicken cells holds great promise for several diverse areas, including developmental biology and biomedical research. To this end, site-specific gene integration has been an attrac... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 21, 2019 Category: Biotechnology Authors: Long Xie, Juanjuan Sun, Lifen Mo, Tianpeng Xu, Qaisar Shahzad, Dongyang Chen, Wenhao Yang, Yuying Liao and Yangqing Lu Tags: Research Source Type: research
Identification of urban particulate matter-induced disruption of human respiratory mucosa integrity using whole transcriptome analysis and organ-on-a chip
Exposure to air particulate matter (PM) is associated with various diseases in the human respiratory system. To date, most in vitro studies showing cellular responses to PM have been performed in cell culture ... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 15, 2019 Category: Biotechnology Authors: Junhyoung Byun, Boa Song, Kyungwoo Lee, Byoungjae Kim, Hae Won Hwang, Myung-Ryul Ok, Hojeong Jeon, Kijeong Lee, Seung-Kuk Baek, Sang-Heon Kim, Seung Ja Oh and Tae Hoon Kim Tags: Research Source Type: research
Antigen-labeled mesoporous silica-coated Au-core Pt-shell nanostructure: a novel nanoprobe for highly efficient virus diagnosis
As an emerging research area of artificial enzymes, nanozyme, the catalytic nanomaterials with enzyme-like characteristics, have attracted enormous attention in research. Here, a nanozyme probe has been realiz... (Source: Journal of Biological Engineering)
Source: Journal of Biological Engineering - November 14, 2019 Category: Biotechnology Authors: Aiyun Li, Lin Long, Fengshou Liu, Jianbo Liu, Xiaochun Wu and Yinglu Ji Tags: Research Source Type: research
