Posttranslational modifications of keratins and their associated proteins as therapeutic targets in keratin diseases
Curr Opin Cell Biol. 2023 Nov 3;85:102264. doi: 10.1016/j.ceb.2023.102264. Online ahead of print.ABSTRACTThe keratin cytoskeleton protects epithelia against mechanical, nonmechanical, and physical stresses, and participates in multiple signaling pathways that regulate cell integrity and resilience. Keratin gene mutations cause multiple rare monoallelic epithelial diseases termed keratinopathies, including the skin diseases Epidermolysis Bullosa Simplex (EBS) and Pachyonychia Congenita (PC), with limited available therapies. The disease-related keratin mutations trigger posttranslational modifications (PTMs) in keratins and...
Source: Current Opinion in Cell Biology - November 5, 2023 Category: Cytology Authors: Pei Li Katrin Rietscher Henriette Jopp Thomas M Magin M Bishr Omary Source Type: research
Posttranslational modifications of keratins and their associated proteins as therapeutic targets in keratin diseases
Curr Opin Cell Biol. 2023 Nov 3;85:102264. doi: 10.1016/j.ceb.2023.102264. Online ahead of print.ABSTRACTThe keratin cytoskeleton protects epithelia against mechanical, nonmechanical, and physical stresses, and participates in multiple signaling pathways that regulate cell integrity and resilience. Keratin gene mutations cause multiple rare monoallelic epithelial diseases termed keratinopathies, including the skin diseases Epidermolysis Bullosa Simplex (EBS) and Pachyonychia Congenita (PC), with limited available therapies. The disease-related keratin mutations trigger posttranslational modifications (PTMs) in keratins and...
Source: Current Opinion in Cell Biology - November 5, 2023 Category: Cytology Authors: Pei Li Katrin Rietscher Henriette Jopp Thomas M Magin M Bishr Omary Source Type: research
Novel roles for cooperating collagen receptor families in fibrotic niches
Curr Opin Cell Biol. 2023 Oct 31;85:102273. doi: 10.1016/j.ceb.2023.102273. Online ahead of print.ABSTRACTRecent data indicate that integrin and non-integrin collagen receptors cooperate in the fibrosis-specific microenvironment (i.e., the fibrotic niche). In certain tumor types, DDR1 can regulate the interaction with collagen III to regulate dormancy and metastasis, whereas in other tumor types, DDR1 can be shed and used to reorganize collagen. DDR1 expressed on tumor cells, together with DDR2 and α11β1 integrin expressed on cancer-associated fibroblasts, can increase tumor tissue stiffness. Integrin α1β1 and α2β1 a...
Source: Current Opinion in Cell Biology - November 2, 2023 Category: Cytology Authors: C édric Zeltz Marion Kusche-Gullberg Ritva Heljasvaara Donald Gullberg Source Type: research
Plasticity of cytoplasmic intermediate filament architecture determines cellular functions
Curr Opin Cell Biol. 2023 Oct 31;85:102270. doi: 10.1016/j.ceb.2023.102270. Online ahead of print.ABSTRACTCytoplasmic intermediate filaments endow cells with mechanical stability. They are subject to changes in morphology and composition if needed. This remodeling encompasses entire cells but can also be restricted to specific intracellular regions. Intermediate filaments thereby support spatially and temporally defined cell type-specific functions. This review focuses on recent advances in our understanding of how intermediate filament dynamics affect the underlying regulatory pathways. We will elaborate on the role of in...
Source: Current Opinion in Cell Biology - November 2, 2023 Category: Cytology Authors: Nicole Schwarz Rudolf E Leube Source Type: research
Novel roles for cooperating collagen receptor families in fibrotic niches
Curr Opin Cell Biol. 2023 Oct 31;85:102273. doi: 10.1016/j.ceb.2023.102273. Online ahead of print.ABSTRACTRecent data indicate that integrin and non-integrin collagen receptors cooperate in the fibrosis-specific microenvironment (i.e., the fibrotic niche). In certain tumor types, DDR1 can regulate the interaction with collagen III to regulate dormancy and metastasis, whereas in other tumor types, DDR1 can be shed and used to reorganize collagen. DDR1 expressed on tumor cells, together with DDR2 and α11β1 integrin expressed on cancer-associated fibroblasts, can increase tumor tissue stiffness. Integrin α1β1 and α2β1 a...
Source: Current Opinion in Cell Biology - November 2, 2023 Category: Cytology Authors: C édric Zeltz Marion Kusche-Gullberg Ritva Heljasvaara Donald Gullberg Source Type: research
Plasticity of cytoplasmic intermediate filament architecture determines cellular functions
Curr Opin Cell Biol. 2023 Oct 31;85:102270. doi: 10.1016/j.ceb.2023.102270. Online ahead of print.ABSTRACTCytoplasmic intermediate filaments endow cells with mechanical stability. They are subject to changes in morphology and composition if needed. This remodeling encompasses entire cells but can also be restricted to specific intracellular regions. Intermediate filaments thereby support spatially and temporally defined cell type-specific functions. This review focuses on recent advances in our understanding of how intermediate filament dynamics affect the underlying regulatory pathways. We will elaborate on the role of in...
Source: Current Opinion in Cell Biology - November 2, 2023 Category: Cytology Authors: Nicole Schwarz Rudolf E Leube Source Type: research
Novel roles for cooperating collagen receptor families in fibrotic niches
Curr Opin Cell Biol. 2023 Oct 31;85:102273. doi: 10.1016/j.ceb.2023.102273. Online ahead of print.ABSTRACTRecent data indicate that integrin and non-integrin collagen receptors cooperate in the fibrosis-specific microenvironment (i.e., the fibrotic niche). In certain tumor types, DDR1 can regulate the interaction with collagen III to regulate dormancy and metastasis, whereas in other tumor types, DDR1 can be shed and used to reorganize collagen. DDR1 expressed on tumor cells, together with DDR2 and α11β1 integrin expressed on cancer-associated fibroblasts, can increase tumor tissue stiffness. Integrin α1β1 and α2β1 a...
Source: Current Opinion in Cell Biology - November 2, 2023 Category: Cytology Authors: C édric Zeltz Marion Kusche-Gullberg Ritva Heljasvaara Donald Gullberg Source Type: research
Plasticity of cytoplasmic intermediate filament architecture determines cellular functions
Curr Opin Cell Biol. 2023 Oct 31;85:102270. doi: 10.1016/j.ceb.2023.102270. Online ahead of print.ABSTRACTCytoplasmic intermediate filaments endow cells with mechanical stability. They are subject to changes in morphology and composition if needed. This remodeling encompasses entire cells but can also be restricted to specific intracellular regions. Intermediate filaments thereby support spatially and temporally defined cell type-specific functions. This review focuses on recent advances in our understanding of how intermediate filament dynamics affect the underlying regulatory pathways. We will elaborate on the role of in...
Source: Current Opinion in Cell Biology - November 2, 2023 Category: Cytology Authors: Nicole Schwarz Rudolf E Leube Source Type: research
Novel roles for cooperating collagen receptor families in fibrotic niches
Curr Opin Cell Biol. 2023 Oct 31;85:102273. doi: 10.1016/j.ceb.2023.102273. Online ahead of print.ABSTRACTRecent data indicate that integrin and non-integrin collagen receptors cooperate in the fibrosis-specific microenvironment (i.e., the fibrotic niche). In certain tumor types, DDR1 can regulate the interaction with collagen III to regulate dormancy and metastasis, whereas in other tumor types, DDR1 can be shed and used to reorganize collagen. DDR1 expressed on tumor cells, together with DDR2 and α11β1 integrin expressed on cancer-associated fibroblasts, can increase tumor tissue stiffness. Integrin α1β1 and α2β1 a...
Source: Current Opinion in Cell Biology - November 2, 2023 Category: Cytology Authors: C édric Zeltz Marion Kusche-Gullberg Ritva Heljasvaara Donald Gullberg Source Type: research
Plasticity of cytoplasmic intermediate filament architecture determines cellular functions
Curr Opin Cell Biol. 2023 Oct 31;85:102270. doi: 10.1016/j.ceb.2023.102270. Online ahead of print.ABSTRACTCytoplasmic intermediate filaments endow cells with mechanical stability. They are subject to changes in morphology and composition if needed. This remodeling encompasses entire cells but can also be restricted to specific intracellular regions. Intermediate filaments thereby support spatially and temporally defined cell type-specific functions. This review focuses on recent advances in our understanding of how intermediate filament dynamics affect the underlying regulatory pathways. We will elaborate on the role of in...
Source: Current Opinion in Cell Biology - November 2, 2023 Category: Cytology Authors: Nicole Schwarz Rudolf E Leube Source Type: research
Live-cell imaging in the deep learning era
Curr Opin Cell Biol. 2023 Oct 26;85:102271. doi: 10.1016/j.ceb.2023.102271. Online ahead of print.ABSTRACTLive imaging is a powerful tool, enabling scientists to observe living organisms in real time. In particular, when combined with fluorescence microscopy, live imaging allows the monitoring of cellular components with high sensitivity and specificity. Yet, due to critical challenges (i.e., drift, phototoxicity, dataset size), implementing live imaging and analyzing the resulting datasets is rarely straightforward. Over the past years, the development of bioimage analysis tools, including deep learning, is changing how w...
Source: Current Opinion in Cell Biology - October 28, 2023 Category: Cytology Authors: Joanna W Pylv änäinen Estibaliz G ómez-de-Mariscal Ricardo Henriques Guillaume Jacquemet Source Type: research
Macromolecular crowding: Sensing without a sensor
Curr Opin Cell Biol. 2023 Oct 26;85:102269. doi: 10.1016/j.ceb.2023.102269. Online ahead of print.ABSTRACTAll living cells are crowded with macromolecules. Crowding can directly modulate biochemical reactions to various degrees depending on the sizes, shapes, and binding affinities of the reactants. Here, we explore the possibility that cells can sense and adapt to changes in crowding through the widespread modulation of biochemical reactions without the need for a dedicated sensor. Additionally, we explore phase separation as a general physicochemical response to changes in crowding, and a mechanism to both transduce info...
Source: Current Opinion in Cell Biology - October 28, 2023 Category: Cytology Authors: Liam J Holt Morgan Delarue Source Type: research
Live-cell imaging in the deep learning era
Curr Opin Cell Biol. 2023 Oct 26;85:102271. doi: 10.1016/j.ceb.2023.102271. Online ahead of print.ABSTRACTLive imaging is a powerful tool, enabling scientists to observe living organisms in real time. In particular, when combined with fluorescence microscopy, live imaging allows the monitoring of cellular components with high sensitivity and specificity. Yet, due to critical challenges (i.e., drift, phototoxicity, dataset size), implementing live imaging and analyzing the resulting datasets is rarely straightforward. Over the past years, the development of bioimage analysis tools, including deep learning, is changing how w...
Source: Current Opinion in Cell Biology - October 28, 2023 Category: Cytology Authors: Joanna W Pylv änäinen Estibaliz G ómez-de-Mariscal Ricardo Henriques Guillaume Jacquemet Source Type: research
Macromolecular crowding: Sensing without a sensor
Curr Opin Cell Biol. 2023 Oct 26;85:102269. doi: 10.1016/j.ceb.2023.102269. Online ahead of print.ABSTRACTAll living cells are crowded with macromolecules. Crowding can directly modulate biochemical reactions to various degrees depending on the sizes, shapes, and binding affinities of the reactants. Here, we explore the possibility that cells can sense and adapt to changes in crowding through the widespread modulation of biochemical reactions without the need for a dedicated sensor. Additionally, we explore phase separation as a general physicochemical response to changes in crowding, and a mechanism to both transduce info...
Source: Current Opinion in Cell Biology - October 28, 2023 Category: Cytology Authors: Liam J Holt Morgan Delarue Source Type: research
Live-cell imaging in the deep learning era
Curr Opin Cell Biol. 2023 Oct 26;85:102271. doi: 10.1016/j.ceb.2023.102271. Online ahead of print.ABSTRACTLive imaging is a powerful tool, enabling scientists to observe living organisms in real time. In particular, when combined with fluorescence microscopy, live imaging allows the monitoring of cellular components with high sensitivity and specificity. Yet, due to critical challenges (i.e., drift, phototoxicity, dataset size), implementing live imaging and analyzing the resulting datasets is rarely straightforward. Over the past years, the development of bioimage analysis tools, including deep learning, is changing how w...
Source: Current Opinion in Cell Biology - October 28, 2023 Category: Cytology Authors: Joanna W Pylv änäinen Estibaliz G ómez-de-Mariscal Ricardo Henriques Guillaume Jacquemet Source Type: research
