Cytoplasmic expression of UTP23 promotes colorectal cancer progression
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):239-245. doi: 10.14715/cmb/2024.70.1.33.ABSTRACTUTP23 (UTP23 small subunit processome component) plays a pivotal role in the intricate processing and maturation of the small subunit of ribosomes within the nucleolus. In cases of nucleolar stress, such as those observed in certain tumor cells, the aberrant nucleolar organization and structure can lead to the translocation of nucleolar proteins into the nucleus or cytoplasm, consequently impacting the physiological processes of the tumor cells through non-ribosome-related functions. Our investigation revealed altered localiza...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Fang E Lili Qian Jinlong Tang Zhaowei Tong Source Type: research
Ginsenoside Rg3 exhibits anti-catabolic and anti-apoptotic effects in IL-1 β treated human disc nucleus pulposus cells and in a rat model of disc degeneration by inactivating the MAPK pathway
This study aimed to investigate the regulatory functions of ginsenoside Rg3 in IDD. We established the IDD cell model via inducing NPCs with IL-1β. The rat model of IDD was established by fibrous ring puncture method. Cell apoptotic capability was assessed through TUNEL assay. The levels of catabolic proteases MMPs and ADAMTSs were tested by western blot and RT-qPCR. IL-1β induction notably promoted the apoptosis of NPCs, while ginsenoside Rg3 treatment reversed the promoting function of IL-1β. Furthermore, we found that MMP2, MMP3, Adamts4, and Adamts5 levels were increased in IL-1β-induced NPCs, while ginsenoside Rg3...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jie Chen Bin Zhang Liwei Wu Jiabin Xu Source Type: research
Inhibition of RXRA-mediated PLA2G2A improves delirium in COPD mice by regulating endoplasmic reticulum stress pathway and inhibiting cell apoptosis
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):226-232. doi: 10.14715/cmb/2024.70.1.31.ABSTRACTDelirium is a common psychiatric complication of chronic obstructive pulmonary disease (COPD). The relief of delirium is considered one of the beneficial ways to treat COPD. However, there are currently no specific drugs that alleviate delirium in COPD patients. Our research aimed to elucidate the specific mechanisms underlying delirium in COPD mice, while also seeking more effective therapeutic targets. In our study, bioinformatics analysis and qRT PCR were used to identify key factors in the development of delirium in COPD a...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Teng Zhang Guodong Wang Source Type: research
Dihydrotestosterone reduces neuroinflammation in spinal cord injury through NF- κB and MAPK pathway
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):213-218. doi: 10.14715/cmb/2024.70.1.29.ABSTRACTNeuroinflammation induced by microglia following spinal cord injury (SCI) leads to secondary neurologic injury. Androgens including testosterone and dihydrotestosterone (DHT) show as endogenous neuroprotective factors against multiple neurologic diseases, while their therapeutic role in SCI-induced neuroinflammation and underlying mechanism remains elusive. In the study, we aimed to investigate the role of DHT against microglia-induced neuroinflammation in SCI and evaluate its protective treatment. BV2 cells were activated by ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jiarui Wei Tao Li Shengyuan Lin Bin Zhang Xing Li Source Type: research
A novel lncRNA TCONS_00071187 upregulated by activated GSK3 β promotes high glucose-induced mesangial cell proliferation in the diabetic nephropathy
In conclusion, GSK3β promoted mesangial cell proliferation by upregulating TCONS_00071187, which led to enhanced ROS production under high glucose conditions in the cell model of DN. This study revealed the role of GSK3β medicated lncRNAs in the development of DN.PMID:38372092 | DOI:10.14715/cmb/2024.70.1.30 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Zhaoshou Yang Xiaoxiao Sun Xiaofeng Deng Wengong Jiang Zhihao Zhang HaiBo Liu Mengdan Zhong Yanfeng Xie Yongdui Ruan Hongmei Lu Source Type: research
Hypo-osmolality activates Wnt/ β-catenin mediated by AQP1 in nucleus pulposus cells degeneration
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):194-199. doi: 10.14715/cmb/2024.70.1.26.ABSTRACTThe goals of this study were to investigate whether Wnt/β-catenin signaling plays a role in hypo-osmolality-related degeneration of nucleus pulposus (NP) cells, and if so, to define the mechanism underlying AQP1 in this effect. Human NP cells were cultured under hypo-osmotic (300/350/400 mOsm) and iso-osmotic (450 mOsm) conditions. The cell viability, AQP1, the expression of Wnt/β-catenin signaling, collagen II/I, and MMP3/9 were evaluated. To determine the effects of the Wnt/β-catenin signaling, we used the inhibitor and t...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Xiao Zhang Zheng Lv Chengjian Wei Source Type: research
Up-regulation of lncRNA WT1-AS ameliorates A β-stimulated neuronal injury through modulation of miR-186-5p/CCND2 axis in Alzheimer's disease
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):200-206. doi: 10.14715/cmb/2024.70.1.27.ABSTRACTAs a common neurodegenerative disorder, Alzheimer's disease (AD) seriously threatens human life. Long non-coding RNAs (lncRNAs) exhibit essential functions in AD development. Nevertheless, the detailed effects and possible mechanisms of lncRNA Wilms tumor 1 Antisense RNA (WT1-AS) in AD are largely unknown. In our studies, a total of 30 serum samples from AD patients were collected, and WT1-AS expressions were detected through qRT-PCR analysis. Additionally, an in vitro AD model was constructed by treating Aβ1-42 in human neur...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Yun Tang Xiaojiu Zhang Shihui Wang Lu Liu Qing Wang Yufeng Liu Yadong Yu Qijin Zhai Source Type: research
Rosmarinic acid attenuates TNF- α induced cardiomyocyte injury via regulating miR-344a-3p
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):207-212. doi: 10.14715/cmb/2024.70.1.28.ABSTRACTTo investigate whether rosmarinic acid protects cardiomyocytes from inflammatory damage through miRNAs, high-throughput sequencing and bioinformatics analysis were performed to identify TNF-α-induced inflammatory damage in cardiomyocytes and miRNAs differentially expressed in TNF-α-induced inflammatory injury in cardiomyocytes, and the bioinformatics analysis shown that the expression levels of 10 miRNAs were significantly up-regulated, and the expression levels of 6 miRNAs were significantly down-regulated. Among them, the ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jun Zhang Yingqiang Du Feili Li Source Type: research
Comprehensive molecular analyses of a 7-m6A-related lncRNAs signature for prognosis, tumor immunity and therapeutic effect in patients with hepatocellular carcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):186-193. doi: 10.14715/cmb/2024.70.1.25.ABSTRACTHepatocellular carcinoma is the most common form of liver tumor. m6A modification and noncoding RNA show indispensable roles in HCC. We sought to establish and verify an appropriate m6A-related long noncoding RNA prognostic tool for predicting hepatocellular carcinoma progression. We extracted the RNA expression levels and the clinicopathologic data from GTEx and TCGA databases. Multivariate Cox regression analysis and receiver operating characteristic curves were performed to test the model's predictive ability. We further bu...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Xiaomin Wu Yi Wang Leilei Tao Yi Zhou Xiaojing Zhang Li Wang Yingchun Zhang Jun Jin Jun Wang Jinbiao Chen Xiaomin Zhang Xiankai Wang Jixian Yu Source Type: research
Exosomal circMACF1 drives PI3K/AKT/mTOR-mediated autophagy suppression in laryngeal squamous cell carcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):179-185. doi: 10.14715/cmb/2024.70.1.24.ABSTRACTLaryngeal squamous cell carcinoma (LSCC) is a common malignant tumor. The regulatory functions of circular RNAs (circRNAs) in cancers have been broadly reported. The hsa_circ_0011773 (circMACF1) is reported to be overexpressed in LSCC tissues, while its biological function in LSCC remains unclear. CircMACF1 expression in LSCC tissues and cells was assessed via RT-qPCR. Exosomes extracted from cells were identified by TEM and NTA. Autophagy-related proteins were tested by western blot. Confocal microscope was employed for analy...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Yingnan Zhu Chuanxin Duan Yunzhi Gui Danxia Chen Xi Su Source Type: research
The role of mitochondria in metastasis development
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):171-178. doi: 10.14715/cmb/2024.70.1.23.ABSTRACTMetastasis is a hallmark of cancer and is responsible for the largest number of cancer-related deaths. However, it remains poorly understood. Recently, evidence has accumulated pointing to the role of mitochondria in the metastatic spread of cancer cells. Mitochondria are dynamic organelles that have significant metabolic activity and are considered signaling centers with biosynthetic, bioenergetic, and signaling functions that control key biological pathways. Also, data were presented that mitochondria can influence all proce...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Alexander V Blagov Anton Y Postnov Victoriya A Khotina Vasily N Sukhorukov Nikolay K Sadykhov Alexander N Orekhov Source Type: research
Investigation of the effect of oligo-xylulose on the intestinal flora under pressure overload in mice based on 16S rRNA gene sequencing
This study aimed to investigate the effects of oligo-xylulose (XOS) on the gut microbiota of mice with heart failure induced by pressure overload. A chronic heart failure mouse model was constructed by pressure overload, and XOS were administered in their diet. The gut microbiota was analyzed using 16S rRNA gene sequencing, and the effects of XOS on the microbiota composition were evaluated. . XOS supplementation improved the balance of intestinal microbiota in mice under pressure overload, increasing the abundance of beneficial bacteria, such as Bifidobacterium and Lactobacillus, while decreasing the abundance of harmful ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jianfeng Long Xiaoke Qi Zhenjie Tang Kang Zhou Source Type: research
An age classification model based on DNA methylation biomarkers of aging in human peripheral blood using random forest and artificial neural network
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):155-163. doi: 10.14715/cmb/2024.70.1.21.ABSTRACTRecent epigenetic studies have revealed a strong association between DNA methylation and aging and lifespan, which changes (increases or decreases) with age. Based on these, the construction of age prediction models associated with DNA methylation levels can be used to infer biological ages closer to the functional state of the organism. We downloaded methylation data from the Gene Expression Omnibus (GEO) public database for normal peripheral blood samples from people of different ages. We grouped the samples according to age...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Ye Li Yukun Yang Jie He Jinxiu Hu Xiangqing Zhu Chuan Tian Mengdie Chen Xiaojuan Zhao Li Ye Hang Pan Xinghua Pan Source Type: research
MiR-27a-3p exacerbates cell migration and invasion in right-sided/left-sided colorectal cancer by targeting TGFBR2/TCF7L2
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):148-154. doi: 10.14715/cmb/2024.70.1.20.ABSTRACTLeft-sided colorectal cancer (LSCC) and right-sided colorectal cancer (RSCC) belong to colorectal cancer happening at different positions, which exhibit different pathogenesis. MicroRNA (miRNA)s are widely known regulators in diverse carcinomas. This research aims to identify a differentially expressed miRNA that simultaneously regulates genes associated with LSCC and RSCC and reveal their regulatory relation in cell migration and invasion. Bioinformatics analyses were conducted to uncover the dysregulated functional genes in ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Lei Bi Yuntao Zhou Yong Zhang Xin Zhang Source Type: research
18s rDNA characterization and morphological investigation of the medicinal leech Hirudo medicinalis from Felaw Pond
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):143-147. doi: 10.14715/cmb/2024.70.1.19.ABSTRACTHirudinea leeches are obligate parasites on a variety of vertebrates and have recently gained attention for their medicinal purposes. The present study aimed to improve the presence of Hirudo medicinalis in Kurdistan and Iraq (especially because it is regarded as a native species in this region). A total of 23 leech specimens were collected from Felaw Pond during January-July 2023. The collected specimens were investigated morphologically and their species were confirmed according to their partial sequence of 18s rDNA. Primers...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Samir Jawdat Bilal Source Type: research
