A novel lncRNA TCONS_00071187 upregulated by activated GSK3 β promotes high glucose-induced mesangial cell proliferation in the diabetic nephropathy
In conclusion, GSK3β promoted mesangial cell proliferation by upregulating TCONS_00071187, which led to enhanced ROS production under high glucose conditions in the cell model of DN. This study revealed the role of GSK3β medicated lncRNAs in the development of DN.PMID:38372092 | DOI:10.14715/cmb/2024.70.1.30 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Zhaoshou Yang Xiaoxiao Sun Xiaofeng Deng Wengong Jiang Zhihao Zhang HaiBo Liu Mengdan Zhong Yanfeng Xie Yongdui Ruan Hongmei Lu Source Type: research

Hypo-osmolality activates Wnt/ β-catenin mediated by AQP1 in nucleus pulposus cells degeneration
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):194-199. doi: 10.14715/cmb/2024.70.1.26.ABSTRACTThe goals of this study were to investigate whether Wnt/β-catenin signaling plays a role in hypo-osmolality-related degeneration of nucleus pulposus (NP) cells, and if so, to define the mechanism underlying AQP1 in this effect. Human NP cells were cultured under hypo-osmotic (300/350/400 mOsm) and iso-osmotic (450 mOsm) conditions. The cell viability, AQP1, the expression of Wnt/β-catenin signaling, collagen II/I, and MMP3/9 were evaluated. To determine the effects of the Wnt/β-catenin signaling, we used the inhibitor and t...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Xiao Zhang Zheng Lv Chengjian Wei Source Type: research

Up-regulation of lncRNA WT1-AS ameliorates A β-stimulated neuronal injury through modulation of miR-186-5p/CCND2 axis in Alzheimer's disease
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):200-206. doi: 10.14715/cmb/2024.70.1.27.ABSTRACTAs a common neurodegenerative disorder, Alzheimer's disease (AD) seriously threatens human life. Long non-coding RNAs (lncRNAs) exhibit essential functions in AD development. Nevertheless, the detailed effects and possible mechanisms of lncRNA Wilms tumor 1 Antisense RNA (WT1-AS) in AD are largely unknown. In our studies, a total of 30 serum samples from AD patients were collected, and WT1-AS expressions were detected through qRT-PCR analysis. Additionally, an in vitro AD model was constructed by treating Aβ1-42 in human neur...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Yun Tang Xiaojiu Zhang Shihui Wang Lu Liu Qing Wang Yufeng Liu Yadong Yu Qijin Zhai Source Type: research

Rosmarinic acid attenuates TNF- α induced cardiomyocyte injury via regulating miR-344a-3p
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):207-212. doi: 10.14715/cmb/2024.70.1.28.ABSTRACTTo investigate whether rosmarinic acid protects cardiomyocytes from inflammatory damage through miRNAs, high-throughput sequencing and bioinformatics analysis were performed to identify TNF-α-induced inflammatory damage in cardiomyocytes and miRNAs differentially expressed in TNF-α-induced inflammatory injury in cardiomyocytes, and the bioinformatics analysis shown that the expression levels of 10 miRNAs were significantly up-regulated, and the expression levels of 6 miRNAs were significantly down-regulated. Among them, the ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jun Zhang Yingqiang Du Feili Li Source Type: research

Comprehensive molecular analyses of a 7-m6A-related lncRNAs signature for prognosis, tumor immunity and therapeutic effect in patients with hepatocellular carcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):186-193. doi: 10.14715/cmb/2024.70.1.25.ABSTRACTHepatocellular carcinoma is the most common form of liver tumor. m6A modification and noncoding RNA show indispensable roles in HCC. We sought to establish and verify an appropriate m6A-related long noncoding RNA prognostic tool for predicting hepatocellular carcinoma progression. We extracted the RNA expression levels and the clinicopathologic data from GTEx and TCGA databases. Multivariate Cox regression analysis and receiver operating characteristic curves were performed to test the model's predictive ability. We further bu...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Xiaomin Wu Yi Wang Leilei Tao Yi Zhou Xiaojing Zhang Li Wang Yingchun Zhang Jun Jin Jun Wang Jinbiao Chen Xiaomin Zhang Xiankai Wang Jixian Yu Source Type: research

Exosomal circMACF1 drives PI3K/AKT/mTOR-mediated autophagy suppression in laryngeal squamous cell carcinoma
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):179-185. doi: 10.14715/cmb/2024.70.1.24.ABSTRACTLaryngeal squamous cell carcinoma (LSCC) is a common malignant tumor. The regulatory functions of circular RNAs (circRNAs) in cancers have been broadly reported. The hsa_circ_0011773 (circMACF1) is reported to be overexpressed in LSCC tissues, while its biological function in LSCC remains unclear. CircMACF1 expression in LSCC tissues and cells was assessed via RT-qPCR. Exosomes extracted from cells were identified by TEM and NTA. Autophagy-related proteins were tested by western blot. Confocal microscope was employed for analy...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Yingnan Zhu Chuanxin Duan Yunzhi Gui Danxia Chen Xi Su Source Type: research

The role of mitochondria in metastasis development
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):171-178. doi: 10.14715/cmb/2024.70.1.23.ABSTRACTMetastasis is a hallmark of cancer and is responsible for the largest number of cancer-related deaths. However, it remains poorly understood. Recently, evidence has accumulated pointing to the role of mitochondria in the metastatic spread of cancer cells. Mitochondria are dynamic organelles that have significant metabolic activity and are considered signaling centers with biosynthetic, bioenergetic, and signaling functions that control key biological pathways. Also, data were presented that mitochondria can influence all proce...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Alexander V Blagov Anton Y Postnov Victoriya A Khotina Vasily N Sukhorukov Nikolay K Sadykhov Alexander N Orekhov Source Type: research

Investigation of the effect of oligo-xylulose on the intestinal flora under pressure overload in mice based on 16S rRNA gene sequencing
This study aimed to investigate the effects of oligo-xylulose (XOS) on the gut microbiota of mice with heart failure induced by pressure overload. A chronic heart failure mouse model was constructed by pressure overload, and XOS were administered in their diet. The gut microbiota was analyzed using 16S rRNA gene sequencing, and the effects of XOS on the microbiota composition were evaluated. . XOS supplementation improved the balance of intestinal microbiota in mice under pressure overload, increasing the abundance of beneficial bacteria, such as Bifidobacterium and Lactobacillus, while decreasing the abundance of harmful ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Jianfeng Long Xiaoke Qi Zhenjie Tang Kang Zhou Source Type: research

An age classification model based on DNA methylation biomarkers of aging in human peripheral blood using random forest and artificial neural network
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):155-163. doi: 10.14715/cmb/2024.70.1.21.ABSTRACTRecent epigenetic studies have revealed a strong association between DNA methylation and aging and lifespan, which changes (increases or decreases) with age. Based on these, the construction of age prediction models associated with DNA methylation levels can be used to infer biological ages closer to the functional state of the organism. We downloaded methylation data from the Gene Expression Omnibus (GEO) public database for normal peripheral blood samples from people of different ages. We grouped the samples according to age...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Ye Li Yukun Yang Jie He Jinxiu Hu Xiangqing Zhu Chuan Tian Mengdie Chen Xiaojuan Zhao Li Ye Hang Pan Xinghua Pan Source Type: research

MiR-27a-3p exacerbates cell migration and invasion in right-sided/left-sided colorectal cancer by targeting TGFBR2/TCF7L2
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):148-154. doi: 10.14715/cmb/2024.70.1.20.ABSTRACTLeft-sided colorectal cancer (LSCC) and right-sided colorectal cancer (RSCC) belong to colorectal cancer happening at different positions, which exhibit different pathogenesis. MicroRNA (miRNA)s are widely known regulators in diverse carcinomas. This research aims to identify a differentially expressed miRNA that simultaneously regulates genes associated with LSCC and RSCC and reveal their regulatory relation in cell migration and invasion. Bioinformatics analyses were conducted to uncover the dysregulated functional genes in ...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Lei Bi Yuntao Zhou Yong Zhang Xin Zhang Source Type: research

18s rDNA characterization and morphological investigation of the medicinal leech Hirudo medicinalis from Felaw Pond
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):143-147. doi: 10.14715/cmb/2024.70.1.19.ABSTRACTHirudinea leeches are obligate parasites on a variety of vertebrates and have recently gained attention for their medicinal purposes. The present study aimed to improve the presence of Hirudo medicinalis in Kurdistan and Iraq (especially because it is regarded as a native species in this region). A total of 23 leech specimens were collected from Felaw Pond during January-July 2023. The collected specimens were investigated morphologically and their species were confirmed according to their partial sequence of 18s rDNA. Primers...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Samir Jawdat Bilal Source Type: research

Effects of the lncRNA MALAT1 gene region rs664589 site mutation on acute myocardial infarction in Chinese Han
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):119-127. doi: 10.14715/cmb/2024.70.1.16.ABSTRACTWe aimed to study the association between the non-coding region of the lncRNA MALAT1 gene, the non-coding region rs664589 C>G variant, and the risk of acute myocardial infarction (AMI) in the Chinese Han population. 165 NSTEMI and 135 STEMI patients were enrolled in the study. An additional 150 healthy individuals were enrolled as the controls. All subjects were analyzed for the MALAT1 rs664589 locus genotype. The receiver operating curve (ROC) was used to determine the effect of MALAT1 rs664589 single nucleotide polymorphi...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Huamin Yu Sijia Tu Chaofeng Shen Xinghua Bai Jindong Sun Mingjuan Shi Cairong Li Hong Yuan Source Type: research

Effect of silencing SSB1 gene on the expression of NBS1 in irradiated rat submandibular gland cells
In this study, we irradiated rat submandibular gland (SMG) cells, which were either infected with a rAdE5-SSB1-1p2-shRNA recombinant adenovirus to silence SSB or a control virus, to explore the effect of IR on the expression NBS1 in the absence of SSB. Our results showed that the SSB1 mRNA transcripts and protein expression of SSB1 and NBS1 initially increased and decreased later with increased doses. The relative expression reached the highest levels when the SMG cells were irradiated with 2Gy of IR. Silencing the SSB1 gene suppressed the expression of both SSB1 and NBS1 regardless of irradiation. The expression of NBS1 d...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Linjing Gao Qiuli Lv Yude Huang Yiyang Fan Lixiang Zhao Yanfei Zhao Xian Wang Dongqin Mo Haoyu Lu Daiyou Wang Source Type: research

Identification and validation of a novel 9-gene signature of non-specific classification to predict prognosis in glioma patients
In conclusion, our study provides a robust 9-gene signature and a predictive nomogram for evaluating the prognosis of glioma patients, offering valuable insights into personalized treatment strategies.PMID:38372105 | DOI:10.14715/cmb/2024.70.1.18 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Guangzhao Li Xiaowang Niu Xiang Li Bin Lin Fei Yang Zhong Wang Source Type: research

Clinical significance of miR-200a in systemic lupus erythematosus and renal damage in children
Cell Mol Biol (Noisy-le-grand). 2024 Jan 31;70(1):94-98. doi: 10.14715/cmb/2024.70.1.13.ABSTRACTThe purpose was to analyze the clinical significance of miR-200a in children with initially diagnosed SLE and renal damage. Children with initially diagnosed SLE (n=100) and healthy children (n=50) undergoing physical examinations during the same period were recruited. Disease activity of SLE children was determined based on SLEDAI (systemic lupus erythematosus disease activity index), and they were divided to SLEDAI≤9 group and SLEDAI>9 group, respectively. Moreover, SLE children were divided to LN group and non-LN group b...
Source: Cellular and Molecular Biology - February 19, 2024 Category: Molecular Biology Authors: Haiyan Zhang Xiaoqing Zhou Qinghu Li Min Zheng Source Type: research